Rroxscaffold_1G00065090

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000058
Physical Location & Seq
Forward (+)
86848351 .. 86851992
3642 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00065090.1

Sequence Viewer

Length: 2793 bp
ATGGCAGCAGCACTAACCAACAGTAATCTCACTACAGATGAGTCTGCTCTTCTTGCTCTCAAAGCTCATATCACCAGTGATCCTCATAACATGATCTCCACCAACTGGTCAACCACCACCCCTGTTTGCAACTGGGTTGGTGTTACTTGTGGTGCACGCCACTTTAGAGTGGCTGTCTTGAACCTCTCGTATATGGGACTCACGAGCACCATTCCTCCGGAGCTCGGTTACCTATCGTTCCTTGTTGCACTAAGCCTGAGAAATAACAGTTTTCATGGTACCTTGCCTCTGGAATTGGGTCGTTTGCGCCGATTGAAGCTCATCAGTTTCAGATTCAACAATTTTATGGGAGCCATTCCGTCATGGTTTGGGTCCTTATCCAAACTTGAAACCTTCGATTTGTATGGTAATCGATTTTCAGGCTCCATACCTGCTGCCATCTTCAACTTAACAGCTCTCCAAGTAATCAACCTGAGCTATAACCAGCTATCAGAAATTCCAAACGAGATTGGAGCCTTGAATCAACTGGAGAGGTTGTTTGTGCAGTTCAATGCCTTAGAAGGGCATGTTCCTCTCGCTGTCTTCAACATGTCTTCTTTGATTACTGTGACTTTCTATAGAAACAGTTTGAATGGTAGTCTTCGAGAGAATATATATCAACATCTTCCGAGCATTCAACAGTTGGATTTGGCTCAGAACCAGATTGATGGTCCAATTCCATCCAATATTGGCAACTTAACCCAGATAAGGATGATTGACCTTGGCTGGAACTATTTGACAGGGACTATACCACATGAGATTGGCCATCTTCCAAGTTTAGAGAGATTGAGCCTCGGAATTAATAATCTCAGTGGTCTCATCCCATCCTTGATCTTCAATATTTCCACAATAACATTGATAGATCTTCACGACAATCAGCTCTCAGGCACCCTTCCAGCAAACATAGGCCTTGGGCTTCCAAACCTCCAATTTTTCTTCATAGGAGGAAATAAGCTCAGTCGAGTACTCCCTAAATCCATCTCCAGTGCTTCTCAGCTCATAAGGCTAGAGCTTGGCCAAAACTTATTTTCCGGCTTTATTCCTAGTGAGCTCTGTGCCTTAGCAAACCTTTATCGGCTTGCCTTACACCAGAATAATTTGACCATTGATACTTCTATTCCAGAAGTAAATTTTTTCTCTTGTTTGGCAAATCTTAAAGATCTGAGCACATTAGCCTTGTCACACAATCCTTTAAATGCCAAGCTTTCGGTTTCCTTTAGTAATCTCTCAGCATCACTTGAATTCATTTATTTAGGTGACTGCAACATGAGAGGTAAAATCCCCAATGATATAGGCAACTCTTGCAACTTAACACTCTTAGACCTTGCGGAAAATAAACTGAGTGGACCAGTTCCAACTTCAATGGGAAGATTACAGAATGTCCAAATTTTGGATGTGCATGGAAATAAATTGCAAGGACACATTCTGGATGAACTTTGTCAACTGATCCATCTAAATGAGTTAAGTCTGTATGGGAATCAACTTACTGGTGCCATACCTGCCTGCTTGGGTGATCTAAATGCAGCTCTAAGAGGTCTATTCTTACAGTCCAATTTGTTAACATCCACAATACCATCTACCTTGTGGGGGCTTCAAGATATCTTGCACTTAAACCTTTCATCCAACTCTCTGGTTGGGCCTCTCTCAGAAGGTTTTGGCAATTTGAAAGTTGTCATCGATGTAGATTTATCAAACAACCAACTATCTGGCAGCTTACCAAGCAGTATTCAGAGTCTTCAAAATTTGGTCAATCTGTCCTTGGCAAGTAATAATTTAGAAGGCCCTATTCCTAGTTCCTTTGGAAACTTGCTAAGCTTGGAACGCTTGGATTTATCCCAGAACAATCTATCTGGAATGATTCCAAAGTCTCTAGAAGCACTCTCATATCTCAAGTATCTGAACTTGTCTTTCAACAAACTCCAAGGAGAAATTCCAACAGGCGGACCTTTCACAAACTTGTCTGCTCAATCGTTTGTTTCAAACCGTGGATTCTGTGGTGTATCCCGATTTCATGTTCAACCATGCAAAAGGAAAAGTGGTACATCTATCTTGAAATATGTTATTCCAGGGATCTTTTTTGGCTCAGTTTGTAAAGGAAAATTTTCAGATGGGGTAGATGTGGCCATAAAGGTTTTTAATTTAGAAACAGAAGGGGCTTTCACAAGTTTTGATGTTGAATGTGACATGCTAAGCAATATTCGTCACCGAAATCTTATCAAAGTCATCAGTTGCTGCAATCAAAATGATTTCAAAGCTCTTATCCTGAATTACATGCCTAATCGGAACCTGGAGAAGTGGTTATATTCACAGAACTATTCTTTGAACATGCTGCAGAGGTTGGATATAATGATAAATGTTGCATCAGCTTTGGAGTATCTTCATCACGATTATGAAACACCTATTGTCCATTGTGACTTGAAGCCCAGCAACATATTACTAGATGATGATATGGTTGCACATGTTGCTGACTTTGGCATAGCAAAACTATTAGGCGGAGGTGATTCTATGACTCAAATGATGACGCTAGCGACAATTGGGTATATGGCTCCAGATGCAATAGTTGAAGTTGTTGATACTAATTTACTTGGGACACAGAAAGATCCTAAATTTGTGAGCTATAATGTTTGCTTCTCATCCATTATGATATTAGGTGTTGCTTGTTGTGTAGAGTCACCAGAAGAGAGGATAAATATGCAAGAGGCTGTAGCCACGATGAACAAAATCAAGACCGAGTATGTGAAGGAGGTGTGGTGA

Protein Analysis

930

Amino Acids

101.98

Weight (kDa)

5.85

Isoelectric Point (pI)

33.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 12 - 50 1.2e-10 Leucine rich repeat N-terminal domain
LRR_14 PF23598 60 - 135 1e-05 Leucine-rich repeat region
LRR_8 PF13855 104 - 163 8.5e-08 Leucine rich repeat
LRR_8 PF13855 144 - 186 6.8e-06 Leucine rich repeat
LRR_14 PF23598 540 - 653 2.5e-08 Leucine-rich repeat region
LRR_8 PF13855 594 - 653 3e-09 Leucine rich repeat
Pkinase PF00069 706 - 866 1.1e-28 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 706 - 897 5.4e-27 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000058)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g13810 FvH4_3g07490 FvH4_3g07510 FvH4_3g07541 FvH4_3g10100 FvH4_3g14951 FvH4_3g22681 FvH4_3g37722 FvH4_5g08260 FvH4_5g08280 FvH4_7g09050 FvH4_7g09050
malus_domestica MD00G1192900.v1.1 MD00G1214300.v1.1 MD02G1291600.v1.1 MD02G1291800.v1.1 MD03G1138500.v1.1 MD03G1180000.v1.1 MD03G1180600.v1.1 MD03G1180700.v1.1 MD03G1180800.v1.1 MD03G1180900.v1.1 MD03G1182000.v1.1 MD03G1182800.v1.1 MD03G1280900.v1.1 MD05G1297700.v1.1 MD05G1298100.v1.1 MD05G1298400.v1.1 MD07G1034700.v1.1 MD07G1034900.v1.1 MD07G1035000.v1.1 MD07G1035900.v1.1 MD07G1168700.v1.1 MD07G1235700.v1.1 MD07G1235900.v1.1 MD07G1236200.v1.1 MD07G1236500.v1.1 MD07G1236800.v1.1 MD10G1212700.v1.1 MD10G1277400.v1.1 MD10G1277600.v1.1 MD11G1180300.v1.1 MD13G1193600.v1.1
prunus_persica Prupe.1G074000_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G063200_v2.0.a1 Prupe.4G063300_v2.0.a1 Prupe.4G063400_v2.0.a1 Prupe.4G063600_v2.0.a1 Prupe.4G064200_v2.0.a1 Prupe.4G064400_v2.0.a1 Prupe.4G064500_v2.0.a1 Prupe.4G064600_v2.0.a1 Prupe.4G076800_v2.0.a1 Prupe.6G160800_v2.0.a1 Prupe.6G160900_v2.0.a1 Prupe.6G294300_v2.0.a1 Prupe.7G141600_v2.0.a1
pyrus_communis pycom03g06490 pycom03g13610 pycom03g13640 pycom03g13660 pycom05g27580 pycom05g27600 pycom05g27620 pycom07g02450 pycom10g23160
rosa_chinensis RchiOBHm_Chr1g0336221 RchiOBHm_Chr2g0113611 RchiOBHm_Chr2g0113631 RchiOBHm_Chr2g0113661 RchiOBHm_Chr2g0113761 RchiOBHm_Chr2g0113861 RchiOBHm_Chr2g0113951 RchiOBHm_Chr2g0114021 RchiOBHm_Chr2g0114121 RchiOBHm_Chr2g0114161 RchiOBHm_Chr2g0114221 RchiOBHm_Chr2g0114281 RchiOBHm_Chr2g0114381 RchiOBHm_Chr2g0115761 RchiOBHm_Chr4g0387401 RchiOBHm_Chr4g0402601 RchiOBHm_Chr4g0403091 RchiOBHm_Chr4g0403141 RchiOBHm_Chr4g0403171 RchiOBHm_Chr4g0403191 RchiOBHm_Chr4g0403231 RchiOBHm_Chr4g0403281 RchiOBHm_Chr4g0403311 RchiOBHm_Chr4g0404191 RchiOBHm_Chr4g0404211 RchiOBHm_Chr4g0404231 RchiOBHm_Chr4g0404261 RchiOBHm_Chr4g0404341 RchiOBHm_Chr4g0404451 RchiOBHm_Chr4g0404511 RchiOBHm_Chr4g0404521 RchiOBHm_Chr4g0404541 RchiOBHm_Chr5g0011141 RchiOBHm_Chr5g0011171 RchiOBHm_Chr5g0011191 RchiOBHm_Chr5g0011261 RchiOBHm_Chr5g0011271 RchiOBHm_Chr5g0011291 RchiOBHm_Chr5g0011331 RchiOBHm_Chr5g0011691 RchiOBHm_Chr5g0014361 RchiOBHm_Chr5g0016021 RchiOBHm_Chr5g0040131 RchiOBHm_Chr5g0040431 RchiOBHm_Chr5g0040461 RchiOBHm_Chr5g0040481 RchiOBHm_Chr5g0042071 RchiOBHm_Chr5g0042121 RchiOBHm_Chr5g0059161 RchiOBHm_Chr6g0251871 RchiOBHm_Chr6g0277701 RchiOBHm_Chr6g0277761 RchiOBHm_Chr7g0213281
rosa_laevigata RLG00000001438 RLG00000002815 RLG00000008984 RLG00000008985 RLG00000008988 RLG00000008989 RLG00000008991 RLG00000008997 RLG00000008998 RLG00000009000 RLG00000009002 RLG00000009021 RLG00000009024 RLG00000009025 RLG00000009026 RLG00000013277 RLG00000013282 RLG00000017615 RLG00000018077 RLG00000018079 RLG00000018081 RLG00000018087 RLG00000018089 RLG00000018092 RLG00000018096 RLG00000031799 RLG00000031800 RLG00000031812 RLG00000031817 RLG00000031823 RLG00000031824 RLG00000031825 RLG00000031826 RLG00000031853 RLG00000031856 RLG00000032101 RLG00000032235 RLG00000033958 RLG00000033978 RLG00000033983
rosa_multiflora Rmu_co8051036.1_g000001 Rmu_co8262527.1_g000001 Rmu_sc0000225.1_g000018 Rmu_sc0000299.1_g000020 Rmu_sc0000437.1_g000001 Rmu_sc0000694.1_g000007 Rmu_sc0000881.1_g000004 Rmu_sc0000898.1_g000063 Rmu_sc0000903.1_g000008 Rmu_sc0000929.1_g000012 Rmu_sc0000929.1_g000019 Rmu_sc0000929.1_g000035 Rmu_sc0000929.1_g000036 Rmu_sc0001023.1_g000049 Rmu_sc0001699.1_g000009 Rmu_sc0001715.1_g000036 Rmu_sc0001895.1_g000003 Rmu_sc0002908.1_g000023 Rmu_sc0002990.1_g000008 Rmu_sc0002990.1_g000014 Rmu_sc0003151.1_g000001 Rmu_sc0003242.1_g000015 Rmu_sc0003242.1_g000025 Rmu_sc0003242.1_g000030 Rmu_sc0003539.1_g000012 Rmu_sc0004283.1_g000002 Rmu_sc0004283.1_g000009 Rmu_sc0004283.1_g000019 Rmu_sc0004283.1_g000032 Rmu_sc0004283.1_g000033 Rmu_sc0004283.1_g000046 Rmu_sc0004283.1_g000065 Rmu_sc0004516.1_g000003 Rmu_sc0005187.1_g000002 Rmu_sc0007010.1_g000007 Rmu_sc0007102.1_g000003 Rmu_sc0007109.1_g000006 Rmu_sc0007309.1_g000001 Rmu_sc0007415.1_g000036 Rmu_sc0007415.1_g000037 Rmu_sc0007415.1_g000053 Rmu_sc0009151.1_g000002 Rmu_sc0009639.1_g000006 Rmu_sc0009639.1_g000007 Rmu_sc0009975.1_g000001 Rmu_sc0009975.1_g000009 Rmu_sc0011398.1_g000002 Rmu_sc0011941.1_g000002 Rmu_sc0011941.1_g000006 Rmu_sc0011941.1_g000023 Rmu_sc0011941.1_g000024 Rmu_sc0021845.1_g000001 Rmu_sc0042747.1_g000002 Rmu_ssc0000303.1_g000010 Rmu_ssc0000400.1_g000092
rosa_roxburghii Rroxscaffold_1G00040190 Rroxscaffold_1G00040920 Rroxscaffold_1G00041140 Rroxscaffold_1G00060620 Rroxscaffold_1G00062230 Rroxscaffold_1G00065090 Rroxscaffold_1G00065120 Rroxscaffold_1G00065130 Rroxscaffold_1G00065220 Rroxscaffold_2G00128280 Rroxscaffold_2G00129610 Rroxscaffold_2G00130000 Rroxscaffold_2G00130020 Rroxscaffold_2G00130030 Rroxscaffold_2G00130080 Rroxscaffold_2G00130100 Rroxscaffold_3G00246100 Rroxscaffold_5G00347320 Rroxscaffold_5G00347770 Rroxscaffold_5G00347790 Rroxscaffold_5G00347810 Rroxscaffold_7G00191040
rosa_rugosa Rorug02G0188200 Rorug02G0188400 Rorug02G0188600 Rorug02G0188600 Rorug02G0188900 Rorug02G0189000 Rorug02G0189000 Rorug02G0189400 Rorug02G0190000 Rorug02G0190100 Rorug04G0037000 Rorug04G0040600 Rorug04G0040700 Rorug04G0453900 Rorug04G0454000 Rorug04G0455300 Rorug04G0455500 Rorug04G0455600 Rorug04G0456400 Rorug04G0456500 Rorug04G0456500 Rorug04G0456500 Rorug04G0458500 Rorug05G0017500 Rorug05G0030700 Rorug05G0030700 Rorug05G0183800 Rorug05G0185200 Rorug05G0198700 Rorug05G0584300 Rorug06G0110500 Rorug07G0141000 Rorug07G0213100
rosa_samantha Rh1DG042100 Rh1DG069600 Rh2AG242900 Rh2AG243800 Rh2AG244400 Rh2AG244700 Rh2AG245000 Rh2AG245200 Rh2AG245500 Rh2AG245700 Rh2AG245800 Rh2AG246000 Rh2AG246300 Rh2AG246600 Rh2AG246700 Rh2AG246900 Rh2AG247100 Rh4BG110700 Rh4CG124800 Rh4CG125200 Rh4DG106800 Rh4DG110400 Rh4DG110700 Rh4DG110900 Rh5CG096000 Rh5CG309200 Rh5DG082300 Rh5DG082900 Rh5DG083000 Rh5DG085700 Rh5DG284600 Rh5DG298400 Rh6AG015900 Rh6AG217100 Rh6CG227200 Rh6DG217700 Rh6DG218500 Rh7BG268200 Rh7DG280500
rosa_wichuraiana Rw0G000200 Rw0G001440 Rw0G005670 Rw0G009590 Rw0G011250 Rw0G014650 Rw0G017420 Rw0G017500 Rw0G017870 Rw0G019090 Rw0G019490 Rw1G005510 Rw2G018990 Rw2G019060 Rw2G019110 Rw2G019120 Rw2G019160 Rw2G019200 Rw2G019430 Rw2G029540 Rw3G025270 Rw4G008870 Rw4G009440 Rw4G009450 Rw4G009470 Rw4G009490 Rw4G009530 Rw4G009540 Rw4G009570 Rw5G007640 Rw5G007650 Rw5G010730 Rw5G025450 Rw5G025460 Rw5G032390 Rw5G032420 Rw6G001520 Rw6G015380 Rw6G018950 Rw7G023670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 439, 1546
Acc65I GGTACC 1 cut(s) 278
AccB1I GGYRCC 3 cut(s) 278, 926, 1529
AccB7I CCANNNNNTGG 2 cut(s) 105, 1429
AccIII TCCGGA 1 cut(s) 217
AciI CCGC 3 cut(s) 1367, 1980, 2532
AclWI GGATC 4 cut(s) 74, 1480, 2117, 2633
AcoI YGGCCR 3 cut(s) 802, 1054, 2160
AcsI RAATTY 8 cut(s) 495, 1168, 1280, 1425, 1780, 1968, 2138, 2645
AfaI GTAC 3 cut(s) 280, 1005, 2080
AfiI CCNNNNNNNGG 7 cut(s) 105, 224, 561, 747, 1429, 1626, 1979
AflIII ACRYGT 2 cut(s) 588, 2497
AjnI CCWGG 2 cut(s) 2104, 2325
AjuI GAANNNNNNNTTGG 2 cut(s) 1953, 1985
Alw21I GWGCWC 5 cut(s) 157, 209, 225, 1092, 1208
Alw26I GTCTC 2 cut(s) 860, 1911
Alw44I GTGCAC 1 cut(s) 153
AlwI GGATC 4 cut(s) 74, 1480, 2117, 2633
AlwNI CAGNNNCTG 1 cut(s) 2271
Aor13HI TCCGGA 1 cut(s) 217
AoxI GGCC 6 cut(s) 802, 946, 1054, 1676, 1819, 2160
ApaLI GTGCAC 1 cut(s) 153
ApeKI GCWGC 7 cut(s) 5, 8, 434, 1562, 1749, 2271, 2368
ApoI RAATTY 8 cut(s) 495, 1168, 1280, 1425, 1780, 1968, 2138, 2645
AseI ATTAAT 1 cut(s) 840
Asp700I GAANNNNTTC 1 cut(s) 2140
Asp718I GGTACC 1 cut(s) 278
AspLEI GCGC 1 cut(s) 309
AspS9I GGNCC 6 cut(s) 372, 710, 1385, 1676, 1820, 1982
AsuHPI GGTGA 6 cut(s) 64, 1307, 1562, 2234, 2549, 2703
AsuNHI GCTAGC 1 cut(s) 2563
AvaII GGWCC 4 cut(s) 372, 710, 1385, 1982
BaeGI GKGCMC 1 cut(s) 157
BalI TGGCCA 3 cut(s) 804, 1056, 2162
BanI GGYRCC 3 cut(s) 278, 926, 1529
BanII GRGCYC 2 cut(s) 225, 1092
BauI CACGAG 1 cut(s) 202
BbsI GAAGAC 4 cut(s) 574, 585, 632, 1766
Bbv12I GWGCWC 5 cut(s) 157, 209, 225, 1092, 1208
BbvI GCAGC 7 cut(s) 17, 20, 421, 1574, 1761, 2258, 2355
BccI CCATC 9 cut(s) 446, 701, 727, 813, 871, 1025, 1497, 1621, 2141
BciT130I CCWGG 2 cut(s) 2106, 2327
BciVI GTATCC 1 cut(s) 2050
BcoDI GTCTC 2 cut(s) 860, 1911
BfaI CTAG 6 cut(s) 1046, 1083, 1830, 1910, 2477, 2564
BfmI CTRYAG 4 cut(s) 33, 616, 2369, 2742
BfuAI ACCTGC 2 cut(s) 439, 1546
BfuI GTATCC 1 cut(s) 2050
BglII AGATCT 2 cut(s) 901, 1198
BisI GCNGC 7 cut(s) 6, 9, 435, 1563, 1750, 2272, 2369
BlpI GCTNAGC 2 cut(s) 1850, 2228
BlsI GCNGC 7 cut(s) 7, 10, 436, 1564, 1751, 2273, 2370
BmcAI AGTACT 1 cut(s) 1005
Bme1390I CCNGG 2 cut(s) 2106, 2327
Bme18I GGWCC 4 cut(s) 372, 710, 1385, 1982
BmgT120I GGNCC 6 cut(s) 372, 710, 1385, 1676, 1820, 1982
BmiI GGNNCC 9 cut(s) 280, 352, 373, 424, 514, 928, 1531, 2324, 2586
BmrFI CCNGG 2 cut(s) 2106, 2327
BmrI ACTGGG 1 cut(s) 142
BmsI GCATC 3 cut(s) 1280, 2408, 2581
BmtI GCTAGC 1 cut(s) 2567
BmuI ACTGGG 1 cut(s) 142
BpiI GAAGAC 4 cut(s) 574, 585, 632, 1766
BpmI CTGGAG 4 cut(s) 548, 1006, 2348, 2571
Bpu10I CCTNAGC 2 cut(s) 473, 1099
Bpu1102I GCTNAGC 2 cut(s) 1850, 2228
BpuEI CTTGAG 1 cut(s) 1913
Bsa29I ATCGAT 2 cut(s) 412, 1716
BsaI GGTCTC 1 cut(s) 860
BsaJI CCNNGG 7 cut(s) 760, 832, 949, 1797, 1960, 2023, 2105
BsaWI WCCGGW 1 cut(s) 217
BsaXI ACNNNNNCTCC 9 cut(s) 80, 110, 199, 229, 521, 551, 2321, 2351, 2774
Bsc4I CCNNNNNNNGG 7 cut(s) 105, 224, 561, 747, 1429, 1626, 1979
Bse1I ACTGG 7 cut(s) 75, 110, 137, 531, 1023, 1388, 1531
BseAI TCCGGA 1 cut(s) 217
BseBI CCWGG 2 cut(s) 2106, 2327
BseCI ATCGAT 2 cut(s) 412, 1716
BseDI CCNNGG 7 cut(s) 760, 832, 949, 1797, 1960, 2023, 2105
BseGI GGATG 9 cut(s) 719, 756, 858, 863, 1438, 1474, 1601, 1658, 2672
BseLI CCNNNNNNNGG 7 cut(s) 105, 224, 561, 747, 1429, 1626, 1979
BseNI ACTGG 7 cut(s) 75, 110, 137, 531, 1023, 1388, 1531
BseSI GKGCMC 1 cut(s) 157
BseXI GCAGC 7 cut(s) 17, 20, 421, 1574, 1761, 2258, 2355
BseYI CCCAGC 1 cut(s) 2462
BsgI GTGCAG 1 cut(s) 563
BshFI GGCC 6 cut(s) 804, 948, 1056, 1678, 1821, 2162
BshNI GGYRCC 3 cut(s) 278, 926, 1529
BshVI ATCGAT 2 cut(s) 412, 1716
BsiHKAI GWGCWC 5 cut(s) 157, 209, 225, 1092, 1208
BsiSI CCGG 2 cut(s) 218, 1071
BslFI GGGAC 3 cut(s) 210, 796, 2641
BslI CCNNNNNNNGG 7 cut(s) 105, 224, 561, 747, 1429, 1626, 1979
BsmAI GTCTC 2 cut(s) 860, 1911
BsmFI GGGAC 3 cut(s) 210, 796, 2641
BsmI GAATGC 1 cut(s) 672
BsnI GGCC 6 cut(s) 804, 948, 1056, 1678, 1821, 2162
Bso31I GGTCTC 1 cut(s) 860
Bsp1286I GDGCHC 5 cut(s) 157, 209, 225, 1092, 1208
Bsp13I TCCGGA 1 cut(s) 217
Bsp143I GATC 9 cut(s) 79, 93, 870, 901, 1198, 1485, 1552, 2109, 2638
Bsp1720I GCTNAGC 2 cut(s) 1850, 2228
BspACI CCGC 3 cut(s) 1367, 1980, 2532
BspANI GGCC 6 cut(s) 804, 948, 1056, 1678, 1821, 2162
BspDI ATCGAT 2 cut(s) 412, 1716
BspEI TCCGGA 1 cut(s) 217
BspLI GGNNCC 9 cut(s) 280, 352, 373, 424, 514, 928, 1531, 2324, 2586
BspMAI CTGCAG 1 cut(s) 2373
BspMI ACCTGC 2 cut(s) 439, 1546
BspOI GCTAGC 1 cut(s) 2567
BspPI GGATC 4 cut(s) 74, 1480, 2117, 2633
BspQI GCTCTTC 1 cut(s) 54
BspT107I GGYRCC 3 cut(s) 278, 926, 1529
BspTNI GGTCTC 1 cut(s) 860
BsrI ACTGG 7 cut(s) 75, 110, 137, 531, 1023, 1388, 1531
BssECI CCNNGG 7 cut(s) 760, 832, 949, 1797, 1960, 2023, 2105
BssMI GATC 9 cut(s) 79, 93, 870, 901, 1198, 1485, 1552, 2109, 2638
BssSI CACGAG 1 cut(s) 202
BssT1I CCWWGG 4 cut(s) 760, 949, 1797, 1960
Bst2BI CACGAG 1 cut(s) 202
Bst2UI CCWGG 2 cut(s) 2106, 2327
Bst4CI ACNGT 7 cut(s) 23, 269, 607, 626, 681, 1587, 2024
Bst6I CTCTTC 2 cut(s) 54, 2712
BstAPI GCANNNNNTGC 2 cut(s) 1341, 2501
BstC8I GCNNGC 4 cut(s) 157, 1119, 1543, 2565
BstDSI CCRYGG 1 cut(s) 2023
BstEII GGTNACC 1 cut(s) 227
BstF5I GGATG 9 cut(s) 719, 756, 858, 863, 1438, 1474, 1601, 1658, 2672
BstHHI GCGC 1 cut(s) 309
BstKTI GATC 9 cut(s) 82, 96, 873, 904, 1201, 1488, 1555, 2112, 2641
BstMAI GTCTC 2 cut(s) 860, 1911
BstMBI GATC 9 cut(s) 79, 93, 870, 901, 1198, 1485, 1552, 2109, 2638
BstMWI GCNNNNNNNGC 9 cut(s) 53, 62, 1042, 1341, 1538, 1758, 1860, 2501, 2591
BstNI CCWGG 2 cut(s) 2106, 2327
BstNSI RCATGY 6 cut(s) 569, 592, 2227, 2314, 2368, 2501
BstPI GGTNACC 1 cut(s) 227
BstSCI CCNGG 2 cut(s) 2104, 2325
BstSFI CTRYAG 4 cut(s) 33, 616, 2369, 2742
BstSLI GKGCMC 1 cut(s) 157
BstV1I GCAGC 7 cut(s) 17, 20, 421, 1574, 1761, 2258, 2355
BstV2I GAAGAC 4 cut(s) 574, 585, 632, 1766
BstX2I RGATCY 4 cut(s) 901, 1198, 2109, 2638
BstXI CCANNNNNNTGG 3 cut(s) 707, 1669, 1745
BstYI RGATCY 4 cut(s) 901, 1198, 2109, 2638
Bsu15I ATCGAT 2 cut(s) 412, 1716
BsuI GTATCC 1 cut(s) 2050
BsuRI GGCC 6 cut(s) 804, 948, 1056, 1678, 1821, 2162
BsuTUI ATCGAT 2 cut(s) 412, 1716
BtgI CCRYGG 1 cut(s) 2023
BtsCI GGATG 9 cut(s) 719, 756, 858, 863, 1438, 1474, 1601, 1658, 2672
BtsIMutI CAGTG 3 cut(s) 82, 856, 1030
BveI ACCTGC 2 cut(s) 439, 1546
Cac8I GCNNGC 4 cut(s) 157, 1119, 1543, 2565
CaiI CAGNNNCTG 1 cut(s) 2271
CfoI GCGC 1 cut(s) 309
Cfr13I GGNCC 6 cut(s) 372, 710, 1385, 1676, 1820, 1982
ClaI ATCGAT 2 cut(s) 412, 1716
CseI GACGC 1 cut(s) 2569
Csp6I GTAC 3 cut(s) 279, 1004, 2079
CviQI GTAC 3 cut(s) 279, 1004, 2079
DpnI GATC 9 cut(s) 81, 95, 872, 903, 1200, 1487, 1554, 2111, 2640
DpnII GATC 9 cut(s) 79, 93, 870, 901, 1198, 1485, 1552, 2109, 2638
DraI TTTAAA 1 cut(s) 1233
EaeI YGGCCR 3 cut(s) 802, 1054, 2160
Eam1104I CTCTTC 2 cut(s) 54, 2712
EarI CTCTTC 2 cut(s) 54, 2712
EciI GGCGGA 2 cut(s) 1995, 2547
Ecl136II GAGCTC 2 cut(s) 223, 1090
Eco130I CCWWGG 4 cut(s) 760, 949, 1797, 1960
Eco147I AGGCCT 1 cut(s) 948
Eco24I GRGCYC 2 cut(s) 225, 1092
Eco31I GGTCTC 1 cut(s) 860
Eco32I GATATC 1 cut(s) 1639
Eco47I GGWCC 4 cut(s) 372, 710, 1385, 1982
Eco53kI GAGCTC 2 cut(s) 223, 1090
Eco91I GGTNACC 1 cut(s) 227
EcoICRI GAGCTC 2 cut(s) 223, 1090
EcoO109I RGGNCCY 2 cut(s) 372, 1820
EcoO65I GGTNACC 1 cut(s) 227
EcoRI GAATTC 1 cut(s) 1280
EcoRII CCWGG 2 cut(s) 2104, 2325
EcoRV GATATC 1 cut(s) 1639
EcoT14I CCWWGG 4 cut(s) 760, 949, 1797, 1960
EcoT38I GRGCYC 2 cut(s) 225, 1092
ErhI CCWWGG 4 cut(s) 760, 949, 1797, 1960
FaqI GGGAC 3 cut(s) 210, 796, 2641
Fnu4HI GCNGC 7 cut(s) 6, 9, 435, 1563, 1750, 2272, 2369
FokI GGATG 9 cut(s) 706, 763, 845, 850, 1445, 1481, 1588, 1645, 2659
FriOI GRGCYC 2 cut(s) 225, 1092
Fsp4HI GCNGC 7 cut(s) 6, 9, 435, 1563, 1750, 2272, 2369
FspBI CTAG 6 cut(s) 1046, 1083, 1830, 1910, 2477, 2564
GlaI GCGC 1 cut(s) 308
GluI GCNGC 7 cut(s) 6, 9, 435, 1563, 1750, 2272, 2369
GsaI CCCAGC 1 cut(s) 2466
GsuI CTGGAG 4 cut(s) 548, 1006, 2348, 2571
HaeIII GGCC 6 cut(s) 804, 948, 1056, 1678, 1821, 2162
HapII CCGG 2 cut(s) 218, 1071
HgaI GACGC 1 cut(s) 2569
HhaI GCGC 1 cut(s) 309
Hin6I GCGC 1 cut(s) 307
HinP1I GCGC 1 cut(s) 307
HincII GTYRAC 3 cut(s) 111, 1481, 1599
HindII GTYRAC 3 cut(s) 111, 1481, 1599
HindIII AAGCTT 2 cut(s) 1241, 1852
HpaI GTTAAC 1 cut(s) 1599
HpaII CCGG 2 cut(s) 218, 1071
HphI GGTGA 6 cut(s) 64, 1307, 1562, 2234, 2549, 2703
Hpy166II GTNNAC 5 cut(s) 111, 155, 1385, 1481, 1599
Hpy8I GTNNAC 5 cut(s) 111, 155, 1385, 1481, 1599
HpyAV CCTTC 7 cut(s) 403, 554, 941, 1682, 1811, 2183, 2773
HpyCH4III ACNGT 7 cut(s) 23, 269, 607, 626, 681, 1587, 2024
HpyF10VI GCNNNNNNNGC 9 cut(s) 53, 62, 1042, 1341, 1538, 1758, 1860, 2501, 2591
HspAI GCGC 1 cut(s) 307
Kpn2I TCCGGA 1 cut(s) 217
KpnI GGTACC 1 cut(s) 282
KspAI GTTAAC 1 cut(s) 1599
Kzo9I GATC 9 cut(s) 79, 93, 870, 901, 1198, 1485, 1552, 2109, 2638
LguI GCTCTTC 1 cut(s) 54
LmnI GCTCC 5 cut(s) 220, 350, 428, 512, 2590
Lsp1109I GCAGC 7 cut(s) 17, 20, 421, 1574, 1761, 2258, 2355
LweI GCATC 3 cut(s) 1280, 2408, 2581
MaeI CTAG 6 cut(s) 1046, 1083, 1830, 1910, 2477, 2564
MaeIII GTNAC 9 cut(s) 142, 227, 607, 1218, 1295, 2219, 2240, 2450, 2709
MalI GATC 9 cut(s) 81, 95, 872, 903, 1200, 1487, 1554, 2111, 2640
MboI GATC 9 cut(s) 79, 93, 870, 901, 1198, 1485, 1552, 2109, 2638
MfeI CAATTG 1 cut(s) 2571
MflI RGATCY 4 cut(s) 901, 1198, 2109, 2638
MhlI GDGCHC 5 cut(s) 157, 209, 225, 1092, 1208
MlsI TGGCCA 3 cut(s) 804, 1056, 2162
MluNI TGGCCA 3 cut(s) 804, 1056, 2162
MlyI GAGTC 5 cut(s) 50, 192, 1780, 2542, 2717
MmeI TCCRAC 5 cut(s) 663, 1418, 1686, 1997, 2358
Mox20I TGGCCA 3 cut(s) 804, 1056, 2162
MroI TCCGGA 1 cut(s) 217
MroXI GAANNNNTTC 1 cut(s) 2140
MscI TGGCCA 3 cut(s) 804, 1056, 2162
MslI CAYNNNNRTG 2 cut(s) 1494, 2427
Msp20I TGGCCA 3 cut(s) 804, 1056, 2162
MspI CCGG 2 cut(s) 218, 1071
MspR9I CCNGG 2 cut(s) 2106, 2327
MunI CAATTG 1 cut(s) 2571
Mva1269I GAATGC 1 cut(s) 672
MvaI CCWGG 2 cut(s) 2106, 2327
MwoI GCNNNNNNNGC 9 cut(s) 53, 62, 1042, 1341, 1538, 1758, 1860, 2501, 2591
NdeII GATC 9 cut(s) 79, 93, 870, 901, 1198, 1485, 1552, 2109, 2638
NheI GCTAGC 1 cut(s) 2563
NlaIV GGNNCC 9 cut(s) 280, 352, 373, 424, 514, 928, 1531, 2324, 2586
NmuCI GTSAC 7 cut(s) 607, 1218, 1295, 2219, 2240, 2450, 2709
NspI RCATGY 6 cut(s) 569, 592, 2227, 2314, 2368, 2501
PceI AGGCCT 1 cut(s) 948
PciI ACATGT 2 cut(s) 588, 2497
PciSI GCTCTTC 1 cut(s) 54
PcsI WCGNNNNNNNCGW 1 cut(s) 307
PctI GAATGC 1 cut(s) 672
PdmI GAANNNNTTC 1 cut(s) 2140
PfeI GAWTC 6 cut(s) 333, 520, 1516, 1897, 2028, 2540
PflMI CCANNNNNTGG 2 cut(s) 105, 1429
PkrI GCNGC 7 cut(s) 7, 10, 436, 1564, 1751, 2273, 2370
PleI GAGTC 5 cut(s) 49, 192, 1779, 2542, 2716
PpsI GAGTC 5 cut(s) 49, 192, 1779, 2542, 2716
PpuMI RGGWCCY 1 cut(s) 372
PscI ACATGT 2 cut(s) 588, 2497
PshBI ATTAAT 1 cut(s) 840
Psp124BI GAGCTC 2 cut(s) 225, 1092
Psp5II RGGWCCY 1 cut(s) 372
Psp6I CCWGG 2 cut(s) 2104, 2325
PspEI GGTNACC 1 cut(s) 227
PspFI CCCAGC 1 cut(s) 2462
PspGI CCWGG 2 cut(s) 2104, 2325
PspN4I GGNNCC 9 cut(s) 280, 352, 373, 424, 514, 928, 1531, 2324, 2586
PspPI GGNCC 6 cut(s) 372, 710, 1385, 1676, 1820, 1982
PspPPI RGGWCCY 1 cut(s) 372
PstI CTGCAG 1 cut(s) 2373
PstNI CAGNNNCTG 1 cut(s) 2271
PsuI RGATCY 4 cut(s) 901, 1198, 2109, 2638
RsaI GTAC 3 cut(s) 280, 1005, 2080
RsaNI GTAC 3 cut(s) 279, 1004, 2079
RseI CAYNNNNRTG 2 cut(s) 1494, 2427
SacI GAGCTC 2 cut(s) 225, 1092
SapI GCTCTTC 1 cut(s) 54
SatI GCNGC 7 cut(s) 6, 9, 435, 1563, 1750, 2272, 2369
Sau3AI GATC 9 cut(s) 79, 93, 870, 901, 1198, 1485, 1552, 2109, 2638
Sau96I GGNCC 6 cut(s) 372, 710, 1385, 1676, 1820, 1982
ScaI AGTACT 1 cut(s) 1005
SchI GAGTC 5 cut(s) 50, 192, 1780, 2542, 2717
ScrFI CCNGG 2 cut(s) 2106, 2327
SduI GDGCHC 5 cut(s) 157, 209, 225, 1092, 1208
SfaNI GCATC 3 cut(s) 1280, 2408, 2581
SfcI CTRYAG 4 cut(s) 33, 616, 2369, 2742
SinI GGWCC 4 cut(s) 372, 710, 1385, 1982
SmiMI CAYNNNNRTG 2 cut(s) 1494, 2427
SmlI CTYRAG 1 cut(s) 1928
SmoI CTYRAG 1 cut(s) 1928
SseBI AGGCCT 1 cut(s) 948
SsiI CCGC 3 cut(s) 1367, 1980, 2532
SspI AATATT 3 cut(s) 727, 880, 2236
SspMI CTAG 6 cut(s) 1046, 1083, 1830, 1910, 2477, 2564
SstI GAGCTC 2 cut(s) 225, 1092
StuI AGGCCT 1 cut(s) 948
StyD4I CCNGG 2 cut(s) 2104, 2325
StyI CCWWGG 4 cut(s) 760, 949, 1797, 1960
TaaI ACNGT 7 cut(s) 23, 269, 607, 626, 681, 1587, 2024
TaqI TCGA 5 cut(s) 396, 412, 643, 1000, 1716
TaqII GACCGA 1 cut(s) 2783
TatI WGTACW 1 cut(s) 1003
TfiI GAWTC 6 cut(s) 333, 520, 1516, 1897, 2028, 2540
TscAI CASTG 3 cut(s) 82, 856, 1030
TseFI GTSAC 7 cut(s) 607, 1218, 1295, 2219, 2240, 2450, 2709
TseI GCWGC 7 cut(s) 5, 8, 434, 1562, 1749, 2271, 2368
Tsp45I GTSAC 7 cut(s) 607, 1218, 1295, 2219, 2240, 2450, 2709
TspDTI ATGAA 9 cut(s) 263, 967, 1273, 1485, 1647, 2039, 2408, 2445, 2768
TspGWI ACGGA 1 cut(s) 348
TspRI CASTG 3 cut(s) 82, 856, 1030
Van91I CCANNNNNTGG 2 cut(s) 105, 1429
VneI GTGCAC 1 cut(s) 153
VpaK11BI GGWCC 4 cut(s) 372, 710, 1385, 1982
VspI ATTAAT 1 cut(s) 840
XapI RAATTY 8 cut(s) 495, 1168, 1280, 1425, 1780, 1968, 2138, 2645
XbaI TCTAGA 1 cut(s) 1909
XceI RCATGY 6 cut(s) 569, 592, 2227, 2314, 2368, 2501
XcmI CCANNNNNNNNNTGG 1 cut(s) 1620
XmnI GAANNNNTTC 1 cut(s) 2140
XspI CTAG 6 cut(s) 1046, 1083, 1830, 1910, 2477, 2564
ZrmI AGTACT 1 cut(s) 1005
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.