FvH4_5g08280

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000058
Physical Location & Seq
Forward (+)
4800483 .. 4803959
3477 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g08280.t1

Sequence Viewer

Length: 2643 bp
ATGTTGATGCTACGGGTACTCTTTCTTTCTGGAGTTCTGTTACTTCACTGCTCATGCTTGCTTCACCTTGCCATTTCTTCTTCAAACCTTACTGATCAATCAACTCTCCTTGCCATCCAATCCAAACTCACTTTCGACCCTACCAATACTGTCTTGGGTGGTAACTGGACCACTGAGACAAGTTTCTGTAATTGGTTTGGGGTTTCTTGTAGTAAACGAAGGCAAAGAGTCACAGCCTTGAAACTTCCCTATATGGGTCTCCAAGGCACCATTTCCCCTCATATTGGCAACCTCTCCTTCCTAGTCCTTCTTGATCTACAAAATAATAGCTTCACAGGGCCCCTGCCACATGAAATCAGTCGCTTACACCGTCTGAGAACACTTGTAGTCCAAGTTAACAAGTTGGAAGGAATTATTCCTCCAGCTTTGCAGCACTGTCAGAAGCTTGAACTCCTATCACTTTCATATAACAGGCTAACTGGTCAGATACCTAAAGAGTTGGGTTTCTTACCCAAGCTCCGGGGCCTATATCTTGGTGTGAACAATTTTGATGCTGGTAATATTCCAGTATTTCTGGGCAACATTTCAACATTGCAAGCTTTGAGTCTACCGAACGCTGGTCTCACAGGTTCATTCCCTCACAGTCTACTGAACCTATCTTCTCTTGTTACTATATCTCTGTATGAAAATAACATTTCTGGAAGTCTTCATGATATGGATATCTGCCATTATTGGCCTAATATTGAAATCCTTCAATTTGCTGACAACAAATTCAGTGGTAAGCTCCCTTCCAGAATTGACCGATGCAGAGAGATTGTGGTCCTATCTCTCTCAAATAATAGGCACATCGGAAGTATTCCCCAAGAAATTGGCGCCTTAGAAAATCTTGAAGAGCTTCTTCTTGAATTTAATAGCCTAACTGGTTTTATACCTCCCACTATAGGTAACTTGTCGAACTTGGAGAAATTTTTTGTCCAAGGTAATAACCTTACAGGAAGTATTCCGAAGGATTTGGGGCGCCTTTCTAATCTGCAAGGACTCGGTTTGGCAAACAATAGCCTAACCGGTGAAATACCAATTGATATTTACAACATTTCCTCCTTAAAGATTTTCACAGCATCATTCAATTCCCTTTCTGGAACTTTTCCTTCATCAACTAGAGTTTTACTTCCTAATATTGATGGTCTCTATCTTTCTAGCAATCAAATTACTGGAAATATCCCAGCATACTTTTCAAATTTTACCAAACTTCGTTTGCTTGATCTTAGCCAAAATTTACTTTATGGAGCAATACCCATGAGTCTTGGTACCATACAGAATCTCCAGATCCTTAATTTGGGCAGAAATCACTTAACTGGGGAACCTGGAGTTCTTGAACTTAAATTCCTTTCTTCTATATTCAATAGCAGTTCACTGAGGGTTTTGGCTTTGGATGACAATCCTCTCAATGGCACCATGCCTGATTCTTTTAGAAATCAACCTTATTCCCTCCAGAACATTTATGCCCTAAGGTGCCAAATCAAAGGTCACATACCATCATCAATTGGTTTTTTGAAAAACTTGACCTCCCTTGTTTTGTTGAACAACAATATGAGTGGAAATCTTCCATCATCAATTGGGGGATTGGAGATGATGCATAGATTGTATCTCGATAGTAACAATATTGAAGGGGTCATTCCAGGTGAACTTTGTCTTTTGAGGAATCTAGGAGAGCTATTACTATCAAATAACAATTTCTCAGGAGCGATCCCAAATTGTATAGCAAACCTCAGAGGCCTTCAAAAAGTCCTCCTGAATTCCAATAGACTGACGTCAACCATACCAATGAGTCTATGGAGCCTTGAAAATCTCCTGTTCCTGGATTTGTCATCCAATTCCCTTAGTGGAGACTTACCTTCAAGCATGAGAAAGACTGCAGTTCTTCAAATGATCGATTTATCCTCGAATCAAATCAGTGGAACAATTCCAACTGTTATTGGAGAATTTCAAAGCTTGAGCTCTTTAAATTTGTCAAACAACATGTTTGTAGGATCTGTTCCCCAAAGTTTTGGTGATCTAAAAGGACTGGAACTTTTGGACCTATCATACAACACCTTGTCTGGCACGATACCAAAGCCTCTAGAAATGCTCAAATATCTCAAGTATTTGAACTTGTCTTATAACAAGCTCTCGGGAGAGATTCCATCTGAGGGGCCTTTTGCCAACTTCAGTGCACAATCATTCTTGGGGAATAGGGAACTCTGTCAGAGATCACCAAAATTTGGAGTCTCACCTTGCAGGAATCCTAAGTATGGTTCGGAAGGGAAAGTGTCTGCTAAGGGTGACATTTATAGTTTGGGAATTATGTTGTTTGAGATTTTCACAAGAAGGAAACCCACAGATGAACTTTTTGGGGGGGAACTGACTATGAGGCAATGGATAAATGCATCGATTCCAGAAAGGGTGATTGAAGTTCTGGACATTGGTTTGTTGTGCATAGAAGATGGAAGAGATGTCAATTTCCTGGAGAGCATCGTTTTAGGTATCATGGAGTTAGGTTTGGAGTGTACCTTAGAATCACCAGAAGAACGGGTCAACATAAAAGAAGTAGTGAACAAGCTGAACAAAATCAAATTAACACTTCTTCATGACAGACAGGGGTAG

Protein Analysis

881

Amino Acids

96.81

Weight (kDa)

6.72

Isoelectric Point (pI)

44.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 80 - 179 3.1e-09 Leucine-rich repeat region
LRR_8 PF13855 124 - 182 2.9e-08 Leucine rich repeat
LRR_8 PF13855 272 - 330 3.3e-06 Leucine rich repeat
LRR_14 PF23598 275 - 375 3.1e-06 Leucine-rich repeat region
LRR_14 PF23598 335 - 526 7.4e-06 Leucine-rich repeat region
LRR_14 PF23598 513 - 620 1e-05 Leucine-rich repeat region
LRR_8 PF13855 591 - 651 1.2e-06 Leucine rich repeat
LRR_14 PF23598 610 - 722 1.3e-07 Leucine-rich repeat region
LRR_8 PF13855 664 - 723 2.7e-09 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000058)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g13810 FvH4_3g07490 FvH4_3g07510 FvH4_3g07541 FvH4_3g10100 FvH4_3g14951 FvH4_3g22681 FvH4_3g37722 FvH4_5g08260 FvH4_5g08280 FvH4_7g09050 FvH4_7g09050
malus_domestica MD00G1192900.v1.1 MD00G1214300.v1.1 MD02G1291600.v1.1 MD02G1291800.v1.1 MD03G1138500.v1.1 MD03G1180000.v1.1 MD03G1180600.v1.1 MD03G1180700.v1.1 MD03G1180800.v1.1 MD03G1180900.v1.1 MD03G1182000.v1.1 MD03G1182800.v1.1 MD03G1280900.v1.1 MD05G1297700.v1.1 MD05G1298100.v1.1 MD05G1298400.v1.1 MD07G1034700.v1.1 MD07G1034900.v1.1 MD07G1035000.v1.1 MD07G1035900.v1.1 MD07G1168700.v1.1 MD07G1235700.v1.1 MD07G1235900.v1.1 MD07G1236200.v1.1 MD07G1236500.v1.1 MD07G1236800.v1.1 MD10G1212700.v1.1 MD10G1277400.v1.1 MD10G1277600.v1.1 MD11G1180300.v1.1 MD13G1193600.v1.1
prunus_persica Prupe.1G074000_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G044400_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.2G048900_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G057800_v2.0.a1 Prupe.4G063200_v2.0.a1 Prupe.4G063300_v2.0.a1 Prupe.4G063400_v2.0.a1 Prupe.4G063600_v2.0.a1 Prupe.4G064200_v2.0.a1 Prupe.4G064400_v2.0.a1 Prupe.4G064500_v2.0.a1 Prupe.4G064600_v2.0.a1 Prupe.4G076800_v2.0.a1 Prupe.6G160800_v2.0.a1 Prupe.6G160900_v2.0.a1 Prupe.6G294300_v2.0.a1 Prupe.7G141600_v2.0.a1
pyrus_communis pycom03g06490 pycom03g13610 pycom03g13640 pycom03g13660 pycom05g27580 pycom05g27600 pycom05g27620 pycom07g02450 pycom10g23160
rosa_chinensis RchiOBHm_Chr1g0336221 RchiOBHm_Chr2g0113611 RchiOBHm_Chr2g0113631 RchiOBHm_Chr2g0113661 RchiOBHm_Chr2g0113761 RchiOBHm_Chr2g0113861 RchiOBHm_Chr2g0113951 RchiOBHm_Chr2g0114021 RchiOBHm_Chr2g0114121 RchiOBHm_Chr2g0114161 RchiOBHm_Chr2g0114221 RchiOBHm_Chr2g0114281 RchiOBHm_Chr2g0114381 RchiOBHm_Chr2g0115761 RchiOBHm_Chr4g0387401 RchiOBHm_Chr4g0402601 RchiOBHm_Chr4g0403091 RchiOBHm_Chr4g0403141 RchiOBHm_Chr4g0403171 RchiOBHm_Chr4g0403191 RchiOBHm_Chr4g0403231 RchiOBHm_Chr4g0403281 RchiOBHm_Chr4g0403311 RchiOBHm_Chr4g0404191 RchiOBHm_Chr4g0404211 RchiOBHm_Chr4g0404231 RchiOBHm_Chr4g0404261 RchiOBHm_Chr4g0404341 RchiOBHm_Chr4g0404451 RchiOBHm_Chr4g0404511 RchiOBHm_Chr4g0404521 RchiOBHm_Chr4g0404541 RchiOBHm_Chr5g0011141 RchiOBHm_Chr5g0011171 RchiOBHm_Chr5g0011191 RchiOBHm_Chr5g0011261 RchiOBHm_Chr5g0011271 RchiOBHm_Chr5g0011291 RchiOBHm_Chr5g0011331 RchiOBHm_Chr5g0011691 RchiOBHm_Chr5g0014361 RchiOBHm_Chr5g0016021 RchiOBHm_Chr5g0040131 RchiOBHm_Chr5g0040431 RchiOBHm_Chr5g0040461 RchiOBHm_Chr5g0040481 RchiOBHm_Chr5g0042071 RchiOBHm_Chr5g0042121 RchiOBHm_Chr5g0059161 RchiOBHm_Chr6g0251871 RchiOBHm_Chr6g0277701 RchiOBHm_Chr6g0277761 RchiOBHm_Chr7g0213281
rosa_laevigata RLG00000001438 RLG00000002815 RLG00000008984 RLG00000008985 RLG00000008988 RLG00000008989 RLG00000008991 RLG00000008997 RLG00000008998 RLG00000009000 RLG00000009002 RLG00000009021 RLG00000009024 RLG00000009025 RLG00000009026 RLG00000013277 RLG00000013282 RLG00000017615 RLG00000018077 RLG00000018079 RLG00000018081 RLG00000018087 RLG00000018089 RLG00000018092 RLG00000018096 RLG00000031799 RLG00000031800 RLG00000031812 RLG00000031817 RLG00000031823 RLG00000031824 RLG00000031825 RLG00000031826 RLG00000031853 RLG00000031856 RLG00000032101 RLG00000032235 RLG00000033958 RLG00000033978 RLG00000033983
rosa_multiflora Rmu_co8051036.1_g000001 Rmu_co8262527.1_g000001 Rmu_sc0000225.1_g000018 Rmu_sc0000299.1_g000020 Rmu_sc0000437.1_g000001 Rmu_sc0000694.1_g000007 Rmu_sc0000881.1_g000004 Rmu_sc0000898.1_g000063 Rmu_sc0000903.1_g000008 Rmu_sc0000929.1_g000012 Rmu_sc0000929.1_g000019 Rmu_sc0000929.1_g000035 Rmu_sc0000929.1_g000036 Rmu_sc0001023.1_g000049 Rmu_sc0001699.1_g000009 Rmu_sc0001715.1_g000036 Rmu_sc0001895.1_g000003 Rmu_sc0002908.1_g000023 Rmu_sc0002990.1_g000008 Rmu_sc0002990.1_g000014 Rmu_sc0003151.1_g000001 Rmu_sc0003242.1_g000015 Rmu_sc0003242.1_g000025 Rmu_sc0003242.1_g000030 Rmu_sc0003539.1_g000012 Rmu_sc0004283.1_g000002 Rmu_sc0004283.1_g000009 Rmu_sc0004283.1_g000019 Rmu_sc0004283.1_g000032 Rmu_sc0004283.1_g000033 Rmu_sc0004283.1_g000046 Rmu_sc0004283.1_g000065 Rmu_sc0004516.1_g000003 Rmu_sc0005187.1_g000002 Rmu_sc0007010.1_g000007 Rmu_sc0007102.1_g000003 Rmu_sc0007109.1_g000006 Rmu_sc0007309.1_g000001 Rmu_sc0007415.1_g000036 Rmu_sc0007415.1_g000037 Rmu_sc0007415.1_g000053 Rmu_sc0009151.1_g000002 Rmu_sc0009639.1_g000006 Rmu_sc0009639.1_g000007 Rmu_sc0009975.1_g000001 Rmu_sc0009975.1_g000009 Rmu_sc0011398.1_g000002 Rmu_sc0011941.1_g000002 Rmu_sc0011941.1_g000006 Rmu_sc0011941.1_g000023 Rmu_sc0011941.1_g000024 Rmu_sc0021845.1_g000001 Rmu_sc0042747.1_g000002 Rmu_ssc0000303.1_g000010 Rmu_ssc0000400.1_g000092
rosa_roxburghii Rroxscaffold_1G00040190 Rroxscaffold_1G00040920 Rroxscaffold_1G00041140 Rroxscaffold_1G00060620 Rroxscaffold_1G00062230 Rroxscaffold_1G00065090 Rroxscaffold_1G00065120 Rroxscaffold_1G00065130 Rroxscaffold_1G00065220 Rroxscaffold_2G00128280 Rroxscaffold_2G00129610 Rroxscaffold_2G00130000 Rroxscaffold_2G00130020 Rroxscaffold_2G00130030 Rroxscaffold_2G00130080 Rroxscaffold_2G00130100 Rroxscaffold_3G00246100 Rroxscaffold_5G00347320 Rroxscaffold_5G00347770 Rroxscaffold_5G00347790 Rroxscaffold_5G00347810 Rroxscaffold_7G00191040
rosa_rugosa Rorug02G0188200 Rorug02G0188400 Rorug02G0188600 Rorug02G0188600 Rorug02G0188900 Rorug02G0189000 Rorug02G0189000 Rorug02G0189400 Rorug02G0190000 Rorug02G0190100 Rorug04G0037000 Rorug04G0040600 Rorug04G0040700 Rorug04G0453900 Rorug04G0454000 Rorug04G0455300 Rorug04G0455500 Rorug04G0455600 Rorug04G0456400 Rorug04G0456500 Rorug04G0456500 Rorug04G0456500 Rorug04G0458500 Rorug05G0017500 Rorug05G0030700 Rorug05G0030700 Rorug05G0183800 Rorug05G0185200 Rorug05G0198700 Rorug05G0584300 Rorug06G0110500 Rorug07G0141000 Rorug07G0213100
rosa_samantha Rh1DG042100 Rh1DG069600 Rh2AG242900 Rh2AG243800 Rh2AG244400 Rh2AG244700 Rh2AG245000 Rh2AG245200 Rh2AG245500 Rh2AG245700 Rh2AG245800 Rh2AG246000 Rh2AG246300 Rh2AG246600 Rh2AG246700 Rh2AG246900 Rh2AG247100 Rh4BG110700 Rh4CG124800 Rh4CG125200 Rh4DG106800 Rh4DG110400 Rh4DG110700 Rh4DG110900 Rh5CG096000 Rh5CG309200 Rh5DG082300 Rh5DG082900 Rh5DG083000 Rh5DG085700 Rh5DG284600 Rh5DG298400 Rh6AG015900 Rh6AG217100 Rh6CG227200 Rh6DG217700 Rh6DG218500 Rh7BG268200 Rh7DG280500
rosa_wichuraiana Rw0G000200 Rw0G001440 Rw0G005670 Rw0G009590 Rw0G011250 Rw0G014650 Rw0G017420 Rw0G017500 Rw0G017870 Rw0G019090 Rw0G019490 Rw1G005510 Rw2G018990 Rw2G019060 Rw2G019110 Rw2G019120 Rw2G019160 Rw2G019200 Rw2G019430 Rw2G029540 Rw3G025270 Rw4G008870 Rw4G009440 Rw4G009450 Rw4G009470 Rw4G009490 Rw4G009530 Rw4G009540 Rw4G009570 Rw5G007640 Rw5G007650 Rw5G010730 Rw5G025450 Rw5G025460 Rw5G032390 Rw5G032420 Rw6G001520 Rw6G015380 Rw6G018950 Rw7G023670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2160
AatII GACGTC 1 cut(s) 1814
Acc65I GGTACC 1 cut(s) 1307
AccB1I GGYRCC 6 cut(s) 266, 872, 1017, 1307, 1451, 1512
AccB7I CCANNNNNTGG 1 cut(s) 2261
AccI GTMKAC 2 cut(s) 607, 646
AclWI GGATC 3 cut(s) 1321, 1741, 2038
AcuI CTGAAG 1 cut(s) 2191
AcyI GRCGYC 3 cut(s) 873, 1018, 1811
AfaI GTAC 3 cut(s) 18, 1309, 2548
AflIII ACRYGT 1 cut(s) 2019
AgeI ACCGGT 1 cut(s) 1064
AjnI CCWGG 4 cut(s) 1363, 1678, 1857, 2502
AjuI GAANNNNNNNTTGG 2 cut(s) 116, 148
AloI GAACNNNNNNTCC 2 cut(s) 1368, 1400
Alw21I GWGCWC 2 cut(s) 2000, 2215
Alw26I GTCTC 6 cut(s) 170, 263, 626, 1190, 1881, 2272
Alw44I GTGCAC 1 cut(s) 2211
AlwI GGATC 3 cut(s) 1321, 1741, 2038
Ama87I CYCGRG 1 cut(s) 2170
AoxI GGCC 5 cut(s) 338, 523, 734, 1774, 2192
ApaI GGGCCC 1 cut(s) 342
ApaLI GTGCAC 1 cut(s) 2211
ApeKI GCWGC 1 cut(s) 430
ArsI GACNNNNNNTTYG 2 cut(s) 1510, 1542
AsiGI ACCGGT 1 cut(s) 1064
Asp700I GAANNNNTTC 2 cut(s) 750, 894
Asp718I GGTACC 1 cut(s) 1307
AspLEI GCGC 2 cut(s) 875, 1020
AspS9I GGNCC 7 cut(s) 168, 338, 339, 523, 820, 2077, 2192
AsuC2I CCSGG 1 cut(s) 521
AsuHPI GGTGA 9 cut(s) 56, 1079, 1694, 2063, 2244, 2262, 2333, 2455, 2550
AvaI CYCGRG 1 cut(s) 2170
AvaII GGWCC 3 cut(s) 168, 820, 2077
AxyI CCTNAGG 1 cut(s) 1508
BaeGI GKGCMC 2 cut(s) 342, 2215
BanI GGYRCC 6 cut(s) 266, 872, 1017, 1307, 1451, 1512
BanII GRGCYC 2 cut(s) 342, 2000
BarI GAAGNNNNNNTAC 2 cut(s) 772, 804
BbsI GAAGAC 1 cut(s) 698
Bbv12I GWGCWC 2 cut(s) 2000, 2215
BbvI GCAGC 1 cut(s) 442
BccI CCATC 6 cut(s) 122, 1175, 1543, 1615, 2191, 2477
BciT130I CCWGG 4 cut(s) 1365, 1680, 1859, 2504
BclI TGATCA 1 cut(s) 94
BcnI CCSGG 1 cut(s) 521
BcoDI GTCTC 6 cut(s) 170, 263, 626, 1190, 1881, 2272
BfaI CTAG 5 cut(s) 302, 1158, 1197, 1706, 2120
BfmI CTRYAG 2 cut(s) 939, 1914
BfoI RGCGCY 2 cut(s) 876, 1021
BisI GCNGC 1 cut(s) 431
BlsI GCNGC 1 cut(s) 432
Bme1390I CCNGG 5 cut(s) 521, 1365, 1680, 1859, 2504
Bme18I GGWCC 3 cut(s) 168, 820, 2077
BmeT110I CYCGRG 1 cut(s) 2170
BmgT120I GGNCC 7 cut(s) 168, 338, 339, 523, 820, 2077, 2192
BmrFI CCNGG 5 cut(s) 521, 1365, 1680, 1859, 2504
BmrI ACTGGG 1 cut(s) 1365
BmsI GCATC 6 cut(s) 541, 794, 1127, 1623, 2435, 2520
BmuI ACTGGG 1 cut(s) 1365
BoxI GACNNNNGTC 1 cut(s) 1810
BpiI GAAGAC 1 cut(s) 698
BpmI CTGGAG 6 cut(s) 51, 405, 1307, 1386, 1475, 2525
Bpu10I CCTNAGC 1 cut(s) 2316
BpuEI CTTGAG 2 cut(s) 2014, 2123
BpuMI CCSGG 1 cut(s) 521
Bsa29I ATCGAT 2 cut(s) 1932, 2429
BsaBI GATNNNNATC 1 cut(s) 1437
BsaHI GRCGYC 3 cut(s) 873, 1018, 1811
BsaI GGTCTC 3 cut(s) 263, 626, 1190
BsaJI CCNNGG 3 cut(s) 262, 520, 976
BsaWI WCCGGW 1 cut(s) 1064
BsaXI ACNNNNNCTCC 8 cut(s) 501, 531, 1082, 1112, 1305, 1335, 1550, 1580
Bse118I RCCGGY 1 cut(s) 1064
Bse1I ACTGG 7 cut(s) 170, 484, 566, 925, 1216, 1360, 2070
Bse21I CCTNAGG 1 cut(s) 1508
Bse3DI GCAATG 2 cut(s) 590, 2420
Bse8I GATNNNNATC 1 cut(s) 1437
BseBI CCWGG 4 cut(s) 1365, 1680, 1859, 2504
BseCI ATCGAT 2 cut(s) 1932, 2429
BseDI CCNNGG 3 cut(s) 262, 520, 976
BseGI GGATG 3 cut(s) 114, 1438, 1868
BseJI GATNNNNATC 1 cut(s) 1437
BseMI GCAATG 2 cut(s) 590, 2420
BseMII CTCAG 6 cut(s) 165, 365, 1406, 1752, 1783, 2178
BseNI ACTGG 7 cut(s) 170, 484, 566, 925, 1216, 1360, 2070
BseSI GKGCMC 2 cut(s) 342, 2215
BseXI GCAGC 1 cut(s) 442
BseYI CCCAGC 1 cut(s) 1222
BshFI GGCC 5 cut(s) 340, 525, 736, 1776, 2194
BshNI GGYRCC 6 cut(s) 266, 872, 1017, 1307, 1451, 1512
BshTI ACCGGT 1 cut(s) 1064
BshVI ATCGAT 2 cut(s) 1932, 2429
BsiHKAI GWGCWC 2 cut(s) 2000, 2215
BsiHKCI CYCGRG 1 cut(s) 2170
BsiSI CCGG 2 cut(s) 520, 1065
BsmAI GTCTC 6 cut(s) 170, 263, 626, 1190, 1881, 2272
BsnI GGCC 5 cut(s) 340, 525, 736, 1776, 2194
Bso31I GGTCTC 3 cut(s) 263, 626, 1190
BsoBI CYCGRG 1 cut(s) 2170
Bsp120I GGGCCC 1 cut(s) 338
Bsp1286I GDGCHC 3 cut(s) 342, 2000, 2215
Bsp143I GATC 9 cut(s) 94, 313, 1261, 1326, 1746, 1929, 2030, 2053, 2249
BspANI GGCC 5 cut(s) 340, 525, 736, 1776, 2194
BspCNI CTCAG 6 cut(s) 166, 366, 1407, 1751, 1782, 2179
BspDI ATCGAT 2 cut(s) 1932, 2429
BspHI TCATGA 2 cut(s) 709, 2626
BspMAI CTGCAG 1 cut(s) 1918
BspPI GGATC 3 cut(s) 1321, 1741, 2038
BspQI GCTCTTC 1 cut(s) 885
BspT107I GGYRCC 6 cut(s) 266, 872, 1017, 1307, 1451, 1512
BspTNI GGTCTC 3 cut(s) 263, 626, 1190
BsrDI GCAATG 2 cut(s) 590, 2420
BsrFI RCCGGY 1 cut(s) 1064
BsrI ACTGG 7 cut(s) 170, 484, 566, 925, 1216, 1360, 2070
BssAI RCCGGY 1 cut(s) 1064
BssECI CCNNGG 3 cut(s) 262, 520, 976
BssMI GATC 9 cut(s) 94, 313, 1261, 1326, 1746, 1929, 2030, 2053, 2249
BssNI GRCGYC 3 cut(s) 873, 1018, 1811
BssT1I CCWWGG 2 cut(s) 262, 976
Bst2UI CCWGG 4 cut(s) 1365, 1680, 1859, 2504
Bst4CI ACNGT 5 cut(s) 151, 371, 437, 644, 1972
Bst6I CTCTTC 2 cut(s) 885, 2482
BstACI GRCGYC 3 cut(s) 873, 1018, 1811
BstC8I GCNNGC 2 cut(s) 59, 597
BstF5I GGATG 3 cut(s) 114, 1438, 1868
BstH2I RGCGCY 2 cut(s) 876, 1021
BstHHI GCGC 2 cut(s) 875, 1020
BstKTI GATC 9 cut(s) 97, 316, 1264, 1329, 1749, 1932, 2033, 2056, 2252
BstMAI GTCTC 6 cut(s) 170, 263, 626, 1190, 1881, 2272
BstMBI GATC 9 cut(s) 94, 313, 1261, 1326, 1746, 1929, 2030, 2053, 2249
BstNI CCWGG 4 cut(s) 1365, 1680, 1859, 2504
BstNSI RCATGY 1 cut(s) 2023
BstPAI GACNNNNGTC 1 cut(s) 1810
BstSCI CCNGG 5 cut(s) 519, 1363, 1678, 1857, 2502
BstSFI CTRYAG 2 cut(s) 939, 1914
BstSLI GKGCMC 2 cut(s) 342, 2215
BstV1I GCAGC 1 cut(s) 442
BstV2I GAAGAC 1 cut(s) 698
BstX2I RGATCY 2 cut(s) 1326, 2030
BstXI CCANNNNNNTGG 2 cut(s) 869, 2048
BstYI RGATCY 2 cut(s) 1326, 2030
Bsu15I ATCGAT 2 cut(s) 1932, 2429
Bsu36I CCTNAGG 1 cut(s) 1508
BsuRI GGCC 5 cut(s) 340, 525, 736, 1776, 2194
BsuTUI ATCGAT 2 cut(s) 1932, 2429
BtsCI GGATG 3 cut(s) 114, 1438, 1868
BtsI GCAGTG 1 cut(s) 46
BtsIMutI CAGTG 7 cut(s) 46, 171, 433, 781, 1412, 1960, 2215
Cac8I GCNNGC 2 cut(s) 59, 597
CciI TCATGA 2 cut(s) 709, 2626
CfoI GCGC 2 cut(s) 875, 1020
Cfr10I RCCGGY 1 cut(s) 1064
Cfr13I GGNCC 7 cut(s) 168, 338, 339, 523, 820, 2077, 2192
ClaI ATCGAT 2 cut(s) 1932, 2429
Csp6I GTAC 3 cut(s) 17, 1308, 2547
CspAI ACCGGT 1 cut(s) 1064
CspCI CAANNNNNGTGG 4 cut(s) 757, 792, 1576, 1611
CviAII CATG 9 cut(s) 54, 350, 710, 1297, 1456, 1903, 2020, 2527, 2627
CviQI GTAC 3 cut(s) 17, 1308, 2547
DinI GGCGCC 2 cut(s) 874, 1019
DpnI GATC 9 cut(s) 96, 315, 1263, 1328, 1748, 1931, 2032, 2055, 2251
DpnII GATC 9 cut(s) 94, 313, 1261, 1326, 1746, 1929, 2030, 2053, 2249
DraI TTTAAA 1 cut(s) 2004
Eam1104I CTCTTC 2 cut(s) 885, 2482
EarI CTCTTC 2 cut(s) 885, 2482
Ecl136II GAGCTC 1 cut(s) 1998
Eco130I CCWWGG 2 cut(s) 262, 976
Eco147I AGGCCT 1 cut(s) 1776
Eco24I GRGCYC 2 cut(s) 342, 2000
Eco31I GGTCTC 3 cut(s) 263, 626, 1190
Eco32I GATATC 1 cut(s) 721
Eco47I GGWCC 3 cut(s) 168, 820, 2077
Eco53kI GAGCTC 1 cut(s) 1998
Eco57I CTGAAG 1 cut(s) 2191
Eco81I CCTNAGG 1 cut(s) 1508
Eco88I CYCGRG 1 cut(s) 2170
EcoICRI GAGCTC 1 cut(s) 1998
EcoO109I RGGNCCY 4 cut(s) 338, 339, 523, 2192
EcoRI GAATTC 1 cut(s) 1795
EcoRII CCWGG 4 cut(s) 1363, 1678, 1857, 2502
EcoRV GATATC 1 cut(s) 721
EcoT14I CCWWGG 2 cut(s) 262, 976
EcoT22I ATGCAT 2 cut(s) 1638, 2428
EcoT38I GRGCYC 2 cut(s) 342, 2000
EgeI GGCGCC 2 cut(s) 874, 1019
EheI GGCGCC 2 cut(s) 874, 1019
ErhI CCWWGG 2 cut(s) 262, 976
FaeI CATG 9 cut(s) 57, 353, 713, 1300, 1459, 1906, 2023, 2530, 2630
FalI AAGNNNNNCTT 2 cut(s) 882, 914
FatI CATG 9 cut(s) 53, 349, 709, 1296, 1455, 1902, 2019, 2526, 2626
FbaI TGATCA 1 cut(s) 94
FblI GTMKAC 2 cut(s) 607, 646
Fnu4HI GCNGC 1 cut(s) 431
FokI GGATG 3 cut(s) 101, 1445, 1855
FriOI GRGCYC 2 cut(s) 342, 2000
Fsp4HI GCNGC 1 cut(s) 431
FspBI CTAG 5 cut(s) 302, 1158, 1197, 1706, 2120
GlaI GCGC 2 cut(s) 874, 1019
GluI GCNGC 1 cut(s) 431
GsaI CCCAGC 1 cut(s) 1226
GsuI CTGGAG 6 cut(s) 51, 405, 1307, 1386, 1475, 2525
HaeII RGCGCY 2 cut(s) 876, 1021
HaeIII GGCC 5 cut(s) 340, 525, 736, 1776, 2194
HapII CCGG 2 cut(s) 520, 1065
HhaI GCGC 2 cut(s) 875, 1020
Hin1I GRCGYC 3 cut(s) 873, 1018, 1811
Hin1II CATG 9 cut(s) 57, 353, 713, 1300, 1459, 1906, 2023, 2530, 2630
Hin6I GCGC 2 cut(s) 873, 1018
HinP1I GCGC 2 cut(s) 873, 1018
HincII GTYRAC 3 cut(s) 397, 1815, 2575
HindII GTYRAC 3 cut(s) 397, 1815, 2575
HindIII AAGCTT 3 cut(s) 443, 597, 1990
HpaI GTTAAC 1 cut(s) 397
HpaII CCGG 2 cut(s) 520, 1065
HphI GGTGA 9 cut(s) 56, 1079, 1694, 2063, 2244, 2262, 2333, 2455, 2550
Hpy188I TCNGA 9 cut(s) 375, 441, 486, 851, 1005, 1772, 2188, 2247, 2299
HpyCH4III ACNGT 5 cut(s) 151, 371, 437, 644, 1972
HpyCH4IV ACGT 1 cut(s) 1811
HpySE526I ACGT 1 cut(s) 1811
Hsp92I GRCGYC 3 cut(s) 873, 1018, 1811
Hsp92II CATG 9 cut(s) 57, 353, 713, 1300, 1459, 1906, 2023, 2530, 2630
HspAI GCGC 2 cut(s) 873, 1018
KasI GGCGCC 2 cut(s) 872, 1017
KpnI GGTACC 1 cut(s) 1311
Ksp22I TGATCA 1 cut(s) 94
KspAI GTTAAC 1 cut(s) 397
Kzo9I GATC 9 cut(s) 94, 313, 1261, 1326, 1746, 1929, 2030, 2053, 2249
LguI GCTCTTC 1 cut(s) 885
LmnI GCTCC 5 cut(s) 522, 789, 1286, 1742, 1836
Lsp1109I GCAGC 1 cut(s) 442
LweI GCATC 6 cut(s) 541, 794, 1127, 1623, 2435, 2520
MaeI CTAG 5 cut(s) 302, 1158, 1197, 1706, 2120
MaeII ACGT 1 cut(s) 1811
MaeIII GTNAC 8 cut(s) 39, 161, 229, 667, 944, 1526, 1655, 2321
MalI GATC 9 cut(s) 96, 315, 1263, 1328, 1748, 1931, 2032, 2055, 2251
MboI GATC 9 cut(s) 94, 313, 1261, 1326, 1746, 1929, 2030, 2053, 2249
MfeI CAATTG 3 cut(s) 1077, 1542, 1614
MflI RGATCY 2 cut(s) 1326, 2030
MhlI GDGCHC 3 cut(s) 342, 2000, 2215
Mly113I GGCGCC 2 cut(s) 873, 1018
MlyI GAGTC 6 cut(s) 237, 613, 1032, 1309, 1837, 2274
MmeI TCCRAC 2 cut(s) 384, 1991
Mph1103I ATGCAT 2 cut(s) 1638, 2428
MroXI GAANNNNTTC 2 cut(s) 750, 894
MseI TTAA 8 cut(s) 396, 909, 1103, 1332, 1352, 1380, 2003, 2615
MslI CAYNNNNRTG 1 cut(s) 1823
MspI CCGG 2 cut(s) 520, 1065
MspR9I CCNGG 5 cut(s) 521, 1365, 1680, 1859, 2504
MunI CAATTG 3 cut(s) 1077, 1542, 1614
MvaI CCWGG 4 cut(s) 1365, 1680, 1859, 2504
NarI GGCGCC 2 cut(s) 873, 1018
NciI CCSGG 1 cut(s) 521
NdeII GATC 9 cut(s) 94, 313, 1261, 1326, 1746, 1929, 2030, 2053, 2249
NlaIII CATG 9 cut(s) 57, 353, 713, 1300, 1459, 1906, 2023, 2530, 2630
NmuCI GTSAC 3 cut(s) 229, 1526, 2321
NsiI ATGCAT 2 cut(s) 1638, 2428
NspI RCATGY 1 cut(s) 2023
PagI TCATGA 2 cut(s) 709, 2626
PceI AGGCCT 1 cut(s) 1776
PciI ACATGT 1 cut(s) 2019
PciSI GCTCTTC 1 cut(s) 885
PcsI WCGNNNNNNNCGW 1 cut(s) 367
PdmI GAANNNNTTC 2 cut(s) 750, 894
PfeI GAWTC 8 cut(s) 1318, 1463, 1702, 1945, 2179, 2281, 2431, 2555
PflMI CCANNNNNTGG 1 cut(s) 2261
PfoI TCCNGGA 2 cut(s) 1857, 2502
PinAI ACCGGT 1 cut(s) 1064
PkrI GCNGC 1 cut(s) 432
PleI GAGTC 6 cut(s) 236, 612, 1032, 1308, 1836, 2273
PluTI GGCGCC 2 cut(s) 876, 1021
PpsI GAGTC 6 cut(s) 236, 612, 1032, 1308, 1836, 2273
PscI ACATGT 1 cut(s) 2019
PshAI GACNNNNGTC 1 cut(s) 1810
PsiI TTATAA 1 cut(s) 2160
Psp124BI GAGCTC 1 cut(s) 2000
Psp6I CCWGG 4 cut(s) 1363, 1678, 1857, 2502
PspFI CCCAGC 1 cut(s) 1222
PspGI CCWGG 4 cut(s) 1363, 1678, 1857, 2502
PspOMI GGGCCC 1 cut(s) 338
PspPI GGNCC 7 cut(s) 168, 338, 339, 523, 820, 2077, 2192
PsrI GAACNNNNNNTAC 2 cut(s) 2019, 2051
PstI CTGCAG 1 cut(s) 1918
PsuI RGATCY 2 cut(s) 1326, 2030
RsaI GTAC 3 cut(s) 18, 1309, 2548
RsaNI GTAC 3 cut(s) 17, 1308, 2547
RseI CAYNNNNRTG 1 cut(s) 1823
SacI GAGCTC 1 cut(s) 2000
SapI GCTCTTC 1 cut(s) 885
SaqAI TTAA 8 cut(s) 396, 909, 1103, 1332, 1352, 1380, 2003, 2615
SatI GCNGC 1 cut(s) 431
Sau3AI GATC 9 cut(s) 94, 313, 1261, 1326, 1746, 1929, 2030, 2053, 2249
Sau96I GGNCC 7 cut(s) 168, 338, 339, 523, 820, 2077, 2192
SchI GAGTC 6 cut(s) 237, 613, 1032, 1309, 1837, 2274
ScrFI CCNGG 5 cut(s) 521, 1365, 1680, 1859, 2504
SduI GDGCHC 3 cut(s) 342, 2000, 2215
SfaNI GCATC 6 cut(s) 541, 794, 1127, 1623, 2435, 2520
SfcI CTRYAG 2 cut(s) 939, 1914
SfoI GGCGCC 2 cut(s) 874, 1019
SinI GGWCC 3 cut(s) 168, 820, 2077
SmiMI CAYNNNNRTG 1 cut(s) 1823
SmlI CTYRAG 2 cut(s) 1993, 2138
SmoI CTYRAG 2 cut(s) 1993, 2138
SseBI AGGCCT 1 cut(s) 1776
SspDI GGCGCC 2 cut(s) 872, 1017
SspI AATATT 4 cut(s) 562, 742, 1177, 1663
SspMI CTAG 5 cut(s) 302, 1158, 1197, 1706, 2120
SstI GAGCTC 1 cut(s) 2000
StuI AGGCCT 1 cut(s) 1776
StyD4I CCNGG 5 cut(s) 519, 1363, 1678, 1857, 2502
StyI CCWWGG 2 cut(s) 262, 976
TaaI ACNGT 5 cut(s) 151, 371, 437, 644, 1972
TaiI ACGT 1 cut(s) 1814
TaqI TCGA 6 cut(s) 135, 953, 1650, 1932, 1943, 2429
TaqII GACCGA 1 cut(s) 816
TfiI GAWTC 8 cut(s) 1318, 1463, 1702, 1945, 2179, 2281, 2431, 2555
Tru1I TTAA 8 cut(s) 396, 909, 1103, 1332, 1352, 1380, 2003, 2615
Tru9I TTAA 8 cut(s) 396, 909, 1103, 1332, 1352, 1380, 2003, 2615
TscAI CASTG 7 cut(s) 53, 178, 440, 781, 1419, 1960, 2215
TseFI GTSAC 3 cut(s) 229, 1526, 2321
TseI GCWGC 1 cut(s) 430
Tsp45I GTSAC 3 cut(s) 229, 1526, 2321
TspDTI ATGAA 8 cut(s) 366, 453, 621, 698, 699, 1140, 2397, 2615
TspRI CASTG 7 cut(s) 53, 178, 440, 781, 1419, 1960, 2215
Van91I CCANNNNNTGG 1 cut(s) 2261
VneI GTGCAC 1 cut(s) 2211
VpaK11BI GGWCC 3 cut(s) 168, 820, 2077
XbaI TCTAGA 1 cut(s) 2119
XceI RCATGY 1 cut(s) 2023
XcmI CCANNNNNNNNNTGG 2 cut(s) 151, 1830
XmiI GTMKAC 2 cut(s) 607, 646
XmnI GAANNNNTTC 2 cut(s) 750, 894
XspI CTAG 5 cut(s) 302, 1158, 1197, 1706, 2120
ZraI GACGTC 1 cut(s) 1812
Zsp2I ATGCAT 2 cut(s) 1638, 2428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.