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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 2 cut(s) | 127, 147 |
| AgsI | TTSAA | 2 cut(s) | 127, 133 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 132, 164 |
| AoxI | GGCC | 1 cut(s) | 88 |
| ApoI | RAATTY | 2 cut(s) | 127, 147 |
| AspS9I | GGNCC | 1 cut(s) | 88 |
| BfaI | CTAG | 1 cut(s) | 75 |
| BmgT120I | GGNCC | 1 cut(s) | 88 |
| BmsI | GCATC | 1 cut(s) | 125 |
| BshFI | GGCC | 1 cut(s) | 90 |
| BsnI | GGCC | 1 cut(s) | 90 |
| BspANI | GGCC | 1 cut(s) | 90 |
| BsuRI | GGCC | 1 cut(s) | 90 |
| BtsIMutI | CAGTG | 1 cut(s) | 152 |
| Cfr13I | GGNCC | 1 cut(s) | 88 |
| CviJI | RGCY | 3 cut(s) | 23, 49, 90 |
| CviKI_1 | RGCY | 3 cut(s) | 23, 49, 90 |
| FspBI | CTAG | 1 cut(s) | 75 |
| HaeIII | GGCC | 1 cut(s) | 90 |
| HinfI | GANTC | 1 cut(s) | 100 |
| Hpy188I | TCNGA | 2 cut(s) | 99, 164 |
| Hpy188III | TCNNGA | 2 cut(s) | 75, 104 |
| LpnPI | CCDG | 2 cut(s) | 28, 66 |
| LweI | GCATC | 1 cut(s) | 125 |
| MaeI | CTAG | 1 cut(s) | 75 |
| MboII | GAAGA | 1 cut(s) | 90 |
| MfeI | CAATTG | 1 cut(s) | 122 |
| MluCI | AATT | 6 cut(s) | 36, 65, 122, 127, 141, 147 |
| MnlI | CCTC | 1 cut(s) | 39 |
| MseI | TTAA | 1 cut(s) | 144 |
| MunI | CAATTG | 1 cut(s) | 122 |
| PfeI | GAWTC | 1 cut(s) | 100 |
| PspPI | GGNCC | 1 cut(s) | 88 |
| SaqAI | TTAA | 1 cut(s) | 144 |
| Sau96I | GGNCC | 1 cut(s) | 88 |
| SetI | ASST | 1 cut(s) | 55 |
| SfaNI | GCATC | 1 cut(s) | 125 |
| SgeI | CNNG | 5 cut(s) | 31, 55, 65, 87, 116 |
| Sse9I | AATT | 6 cut(s) | 36, 65, 122, 127, 141, 147 |
| SspMI | CTAG | 1 cut(s) | 75 |
| TasI | AATT | 6 cut(s) | 36, 65, 122, 127, 141, 147 |
| TfiI | GAWTC | 1 cut(s) | 100 |
| Tru1I | TTAA | 1 cut(s) | 144 |
| Tru9I | TTAA | 1 cut(s) | 144 |
| TscAI | CASTG | 1 cut(s) | 159 |
| TspRI | CASTG | 1 cut(s) | 159 |
| XapI | RAATTY | 2 cut(s) | 127, 147 |
| XbaI | TCTAGA | 1 cut(s) | 74 |
| XspI | CTAG | 1 cut(s) | 75 |