Rorug04G0034400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000267
Physical Location & Seq
Forward (+)
5096301 .. 5099941
3641 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0034400.1

Sequence Viewer

Length: 2343 bp
ATGGAAGGGAAGGCCATGTCCTTGAAAAGTTTGCAATGGGAGGCATCATGGGGGATAAGAGCTCAGCCTCATATTGACTTCATCAACACAGTCCGGGCCCTTCCCATCGATAAGATTTCAGCTATATGGGAGATGGGTTTTGGTTCACTTCAGAGTATAGGATGCCCTCAACTACATGACCAGGTATGGAAAATGATGGTCGACGGCCTTGAACCTCTGAATGAAACAGTGGTTATACATGGAAAGACTTTCAGCATACATGCATCTGATTTTGAGCGTATTATGGGAGTCAGAGACGGGGGACTGGATGTAAGTTTTAGTGGAGATAGTAACACAGCGGTTGTTGCGCATGTCAAACAAGAGTTAGCTGGTTTAGATGGGAACATAACATTATCCTCGTTGAAGGAGGTTGTTCTTCGAAGGGTGGATGTGGACCAAATATTTAGAGTGGCCTACGTATTGTACGCAATGTCGGCCTTATTGTGCCCAACCAATAGTGAAGGGGTCGACTACATATTGCTTATGGCCATGATAACAAGCAGTTCGATAGGGGATCTTAATTGGGCCACATATTGCTTCAGCAAGTTGATGGATGGTGTACTCACAAGGTCGCGCAACGACTATGCCATTGGCGGTGCATGTTTCTTGTTTCTCCAACTGTTTTACTTTGAAGCCGTTGGGACAAGTTTGGGATGGGTTGACAAGACAATCACACCAGTCATATACTGGGGAGTTGACAAGGCCGCAAATTTGGTGCAGTTCGTTAAGAATAATGGAGGCGTTACCTCAGAAAATAACAGAGTCTGGGCACATGAGATAGGTGACACTTGGATGAAAAAGAATGTATTGGAGAACTATGAAGGTGGGATAACAGTGGAGGAAACAGTTTTTGCACAGGTGGAGGAGATGTGGTCAGAGATAGAAAGTGTGAAAAATGATGTGCACTATATGAAGGAAAACGTAGCTCTTATTGCAAAATTGGCTGAAATGATTGAAGGAGTTCGAACTGAGATTTTGTCAGCTAGGAAGGAAACCGCGACCGCTGCTGCCGCAGAGGTATTCAAACAATACAAACCCCAATTAGATCATATGCTTGGTGTGAGACCTAAAGTCATGGAGAGAGCAATAACAGAGGCAAGGAGTAACAGCGCACACTTCAATGGGGGAAAGAATTCTAAATCTAAAGGGGATAATTCACAGGATTCAAGTTGCATGATCTTATATTGCAATAATGTCAATGCACAGACAATACATAATGATCAGGTCAAGGTGAACCGGAACAATGGAAAAGAACTTCAACCAACTCTATCTGATTGTTCAGTGAGCCCCATTGAGGGTCCAAAGGCTATGCGTAGGAGCAGTAAGGAGAAAAATAAAAAGATACCAACTGTGGCACCTAGGCACATTGTTCGTGCACTATTTAAGAAGAGTGCAATTCCGGGTCGAAAGAAGTCACATTTGAAGGGGTGGGGGAAGGGGACTATTGATGCCGGCCCATTCCGTGTCAATCGGGGTACTTCTAAGGACGACATGGATCTTTTGAAGTTTATATTTATGGATATGGAGCAGAATGATTTGGACTATCGTGCCGTAGTTGCGAGTGTCGAAGGAATGCACGTCACAAGGACTGATCTTCAATGCCTTGGTCCAAGGGAGCTTGTCTGCAGCCTTGTTATAAATATATTTGTGGAGTACCTTAACGAAATAGGAAAGGAGGATTGGTATGTCCCAACATACTTCGGGAGAAACTCTATAAAAATGGGAGACAACATGTCAACTCAAGAATGGATTCAACAGACTAAAAGACTAAGTAGGCTTGACAGGTTCATTGGCCAATTTGCCAAATGCAGAAGGATTTTTATCCCAATGCACGAGCCAGAAATACAAGAGAAGGATGCACATTGGTATTTGTTGGTGGTGCATGTCAGAGATAGGTCTGCAGAGATCCTTGATAGTGCTCCAAATCCTTCCAAAACAATATCCCGACAAAGAGATGCACGGGCAGCGTTGGACATGCTGGATCACATTTATGGAGACGCTATGTCGAGACATGCAAATCGGTTGTTTGCCTTCGGAACCCTTCCCTTCAAGGCTGATGGAGCTGGACCTCGACAGAAGAATGATACAGACTGCGGTGTTTTTGTCATTAGGAATATGCAGCGATATGGTATCGATTGGGCTGCTACGTACAATTCGGATATTGAGCGTGCAAGTTTGGTGGTCGAGTGTCTAAGGCATCCGAAGAACGAGGTGGGAGACTTGCATGTGACAGTGGACAGATGCTTAGCTCGCATCTCTGGAAGTAGAGAAGTAAGAGAAACTGCAGTGTTGCCGACCCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

780

Amino Acids

87.59

Weight (kDa)

7.05

Isoelectric Point (pI)

40.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C48 PF02902 590 - 722 1.7e-13 Ulp1 protease family, C-terminal catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000267)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19660 FvH4_1g30321 FvH4_4g07610 FvH4_5g00140 FvH4_6g23340 FvH4_6g29890
rosa_chinensis RchiOBHm_Chr1g0319561 RchiOBHm_Chr1g0320181 RchiOBHm_Chr1g0330531 RchiOBHm_Chr1g0339901 RchiOBHm_Chr1g0341281 RchiOBHm_Chr1g0346381 RchiOBHm_Chr1g0351211 RchiOBHm_Chr1g0358041 RchiOBHm_Chr1g0367541 RchiOBHm_Chr2g0119361 RchiOBHm_Chr2g0120321 RchiOBHm_Chr2g0140171 RchiOBHm_Chr2g0150711 RchiOBHm_Chr2g0151251 RchiOBHm_Chr2g0160901 RchiOBHm_Chr3g0447381 RchiOBHm_Chr3g0459951 RchiOBHm_Chr3g0465101 RchiOBHm_Chr4g0392281 RchiOBHm_Chr4g0405571 RchiOBHm_Chr4g0412841 RchiOBHm_Chr4g0413331 RchiOBHm_Chr4g0422711 RchiOBHm_Chr4g0422751 RchiOBHm_Chr4g0423141 RchiOBHm_Chr4g0423271 RchiOBHm_Chr4g0424711 RchiOBHm_Chr4g0427731 RchiOBHm_Chr4g0442201 RchiOBHm_Chr5g0026201 RchiOBHm_Chr5g0050261 RchiOBHm_Chr6g0277431 RchiOBHm_Chr6g0279711 RchiOBHm_Chr6g0280101 RchiOBHm_Chr6g0280951 RchiOBHm_Chr6g0295101 RchiOBHm_Chr6g0312921 RchiOBHm_Chr7g0201181 RchiOBHm_Chr7g0204591 RchiOBHm_Chr7g0204721 RchiOBHm_Chr7g0204801 RchiOBHm_Chr7g0208411 RchiOBHm_Chr7g0213041 RchiOBHm_Chr7g0215471
rosa_laevigata RLG00000004867 RLG00000018361 RLG00000036656
rosa_multiflora Rmu_sc0000376.1_g000034 Rmu_sc0001576.1_g000002 Rmu_sc0001971.1_g000008 Rmu_sc0002105.1_g000002 Rmu_sc0002310.1_g000018 Rmu_sc0005120.1_g000007 Rmu_sc0005665.1_g000017 Rmu_sc0005715.1_g000010 Rmu_sc0009785.1_g000031 Rmu_sc0010202.1_g000013 Rmu_sc0040921.1_g000004
rosa_roxburghii Rroxscaffold_1G00003120 Rroxscaffold_1G00026660 Rroxscaffold_2G00098060 Rroxscaffold_2G00111130 Rroxscaffold_4G00279230 Rroxscaffold_4G00295650 Rroxscaffold_5G00361140 Rroxscaffold_5G00374320 Rroxscaffold_6G00406950
rosa_rugosa Rorug01G0102500 Rorug01G0165700 Rorug01G0165700 Rorug01G0195800 Rorug01G0195800 Rorug01G0217200 Rorug01G0335400 Rorug02G0029300 Rorug02G0036800 Rorug02G0073300 Rorug02G0279100 Rorug02G0326300 Rorug02G0338400 Rorug02G0371300.1 Rorug02G0421700 Rorug02G0458000 Rorug03G0105100 Rorug03G0105700 Rorug03G0220900 Rorug03G0272600 Rorug03G0322700 Rorug03G0336800 Rorug03G0356900 Rorug03G0364200.1 Rorug04G0028300 Rorug04G0032600 Rorug04G0034400 Rorug04G0203900 Rorug05G0192000 Rorug05G0192000 Rorug05G0313700 Rorug05G0391500 Rorug05G0403500.1 Rorug06G0032700 Rorug06G0174700 Rorug06G0409900 Rorug06G0458800 Rorug07G0114100 Rorug07G0137600 Rorug07G0163900 Rorug07G0244900 Rorug07G0274700 Rorug07G0310400
rosa_samantha Rh1AG070100 Rh1AG070300 Rh1AG070600 Rh1AG070900 Rh1BG181700 Rh3DG183400 Rh4CG044400 Rh4CG163300 Rh5BG000300 Rh6AG036500 Rh7AG328500 Rh7BG204000 Rh7CG345900
rosa_wichuraiana Rw2G022850 Rw6G002520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1676
Acc16I TGCGCA 1 cut(s) 348
AccB1I GGYRCC 1 cut(s) 1393
AccI GTMKAC 2 cut(s) 201, 507
AccII CGCG 2 cut(s) 613, 1037
AciI CCGC 7 cut(s) 338, 633, 744, 1035, 1041, 1050, 2133
AclWI GGATC 4 cut(s) 561, 1542, 1939, 2028
AcoI YGGCCR 2 cut(s) 525, 1831
AcsI RAATTY 2 cut(s) 748, 1171
AcuI CTGAAG 2 cut(s) 134, 562
AfaI GTAC 5 cut(s) 464, 600, 1516, 1694, 2189
AfiI CCNNNNNNNGG 2 cut(s) 1333, 1334
AflIII ACRYGT 1 cut(s) 1770
AhdI GACNNNNNGTC 3 cut(s) 1109, 1771, 2041
AjiI CACGTC 1 cut(s) 1618
AjnI CCWGG 1 cut(s) 180
AjuI GAANNNNNNNTTGG 2 cut(s) 1702, 1734
AluBI AGCT 8 cut(s) 62, 122, 368, 965, 1022, 1657, 2102, 2288
AluI AGCT 8 cut(s) 62, 122, 368, 965, 1022, 1657, 2102, 2288
Alw21I GWGCWC 4 cut(s) 64, 945, 1417, 1960
Alw26I GTCTC 6 cut(s) 288, 1096, 1758, 2028, 2041, 2250
Alw44I GTGCAC 2 cut(s) 941, 1413
AlwI GGATC 4 cut(s) 561, 1542, 1939, 2028
AlwNI CAGNNNCTG 1 cut(s) 804
ApaI GGGCCC 1 cut(s) 100
ApaLI GTGCAC 2 cut(s) 941, 1413
ApeKI GCWGC 6 cut(s) 1043, 1046, 1665, 2003, 2158, 2180
ApoI RAATTY 2 cut(s) 748, 1171
Asp700I GAANNNNTTC 3 cut(s) 999, 1171, 1788
AspA2I CCTAGG 1 cut(s) 1397
AspLEI GCGC 3 cut(s) 349, 615, 1151
AspS9I GGNCC 8 cut(s) 96, 97, 433, 564, 1337, 1493, 1646, 2105
AsuC2I CCSGG 2 cut(s) 95, 1440
AsuHPI GGTGA 2 cut(s) 833, 1282
AsuII TTCGAA 2 cut(s) 418, 1003
AvaII GGWCC 4 cut(s) 433, 1337, 1646, 2105
AvrII CCTAGG 1 cut(s) 1397
BaeGI GKGCMC 5 cut(s) 100, 488, 811, 945, 1417
BalI TGGCCA 2 cut(s) 527, 1833
BanI GGYRCC 1 cut(s) 1393
BanII GRGCYC 3 cut(s) 64, 100, 1328
BauI CACGAG 1 cut(s) 1871
Bbv12I GWGCWC 4 cut(s) 64, 945, 1417, 1960
BbvI GCAGC 6 cut(s) 1030, 1033, 1677, 2015, 2167, 2170
BccI CCATC 8 cut(s) 113, 127, 190, 371, 583, 587, 687, 2090
BceAI ACGGC 3 cut(s) 220, 659, 1574
BcgI CGANNNNNNTGC 4 cut(s) 1391, 1425, 2162, 2196
BciT130I CCWGG 1 cut(s) 182
BclI TGATCA 1 cut(s) 1258
BcnI CCSGG 2 cut(s) 95, 1440
BcoDI GTCTC 6 cut(s) 288, 1096, 1758, 2028, 2041, 2250
BfaI CTAG 2 cut(s) 1023, 1398
BfmI CTRYAG 3 cut(s) 1663, 1938, 2322
BisI GCNGC 8 cut(s) 744, 1044, 1047, 1050, 1666, 2004, 2159, 2181
BlnI CCTAGG 1 cut(s) 1397
BlpI GCTNAGC 2 cut(s) 63, 2284
BlsI GCNGC 8 cut(s) 745, 1045, 1048, 1051, 1667, 2005, 2160, 2182
Bme1390I CCNGG 3 cut(s) 95, 182, 1440
Bme18I GGWCC 4 cut(s) 433, 1337, 1646, 2105
BmeRI GACNNNNNGTC 3 cut(s) 1109, 1771, 2041
BmgBI CACGTC 1 cut(s) 1618
BmgT120I GGNCC 8 cut(s) 96, 97, 433, 564, 1337, 1493, 1646, 2105
BmiI GGNNCC 4 cut(s) 98, 1338, 1395, 2077
BmrFI CCNGG 3 cut(s) 95, 182, 1440
BmrI ACTGGG 1 cut(s) 736
BmsI GCATC 9 cut(s) 53, 152, 272, 1477, 1885, 1984, 2245, 2270, 2301
BmuI ACTGGG 1 cut(s) 736
Bpu1102I GCTNAGC 2 cut(s) 63, 2284
Bpu14I TTCGAA 2 cut(s) 418, 1003
BpuEI CTTGAG 1 cut(s) 1764
BpuMI CCSGG 2 cut(s) 95, 1440
Bsa29I ATCGAT 2 cut(s) 108, 2172
BsaAI YACGTR 2 cut(s) 457, 2187
BsaBI GATNNNNATC 1 cut(s) 1859
BsaI GGTCTC 1 cut(s) 1096
BsaJI CCNNGG 3 cut(s) 1397, 1642, 1649
BsaWI WCCGGW 1 cut(s) 1275
BsaXI ACNNNNNCTCC 6 cut(s) 893, 896, 923, 926, 1706, 1736
Bsc4I CCNNNNNNNGG 2 cut(s) 1333, 1334
Bse118I RCCGGY 1 cut(s) 1490
Bse1I ACTGG 3 cut(s) 309, 716, 731
Bse3DI GCAATG 2 cut(s) 41, 474
Bse8I GATNNNNATC 1 cut(s) 1859
BseBI CCWGG 1 cut(s) 182
BseCI ATCGAT 2 cut(s) 108, 2172
BseDI CCNNGG 3 cut(s) 1397, 1642, 1649
BseGI GGATG 8 cut(s) 167, 313, 433, 598, 698, 837, 1900, 2236
BseJI GATNNNNATC 1 cut(s) 1859
BseLI CCNNNNNNNGG 2 cut(s) 1333, 1334
BseMI GCAATG 2 cut(s) 41, 474
BseMII CTCAG 3 cut(s) 77, 801, 999
BseNI ACTGG 3 cut(s) 309, 716, 731
BseRI GAGGAG 1 cut(s) 917
BseSI GKGCMC 5 cut(s) 100, 488, 811, 945, 1417
BseXI GCAGC 6 cut(s) 1030, 1033, 1677, 2015, 2167, 2170
BsgI GTGCAG 1 cut(s) 776
Bsh1236I CGCG 2 cut(s) 613, 1037
Bsh1285I CGRYCG 1 cut(s) 1041
BshNI GGYRCC 1 cut(s) 1393
BshVI ATCGAT 2 cut(s) 108, 2172
BsiEI CGRYCG 1 cut(s) 1041
BsiHKAI GWGCWC 4 cut(s) 64, 945, 1417, 1960
BsiSI CCGG 4 cut(s) 94, 1276, 1439, 1491
BslFI GGGAC 4 cut(s) 315, 694, 1492, 1712
BslI CCNNNNNNNGG 2 cut(s) 1333, 1334
BsmAI GTCTC 6 cut(s) 288, 1096, 1758, 2028, 2041, 2250
BsmBI CGTCTC 2 cut(s) 288, 2028
BsmFI GGGAC 4 cut(s) 315, 694, 1492, 1712
BsmI GAATGC 1 cut(s) 1617
Bso31I GGTCTC 1 cut(s) 1096
Bsp119I TTCGAA 2 cut(s) 418, 1003
Bsp120I GGGCCC 1 cut(s) 96
Bsp1286I GDGCHC 8 cut(s) 64, 100, 488, 811, 945, 1328, 1417, 1960
Bsp143I GATC 8 cut(s) 553, 1084, 1215, 1258, 1534, 1630, 1944, 2020
Bsp1720I GCTNAGC 2 cut(s) 63, 2284
BspACI CCGC 7 cut(s) 338, 633, 744, 1035, 1041, 1050, 2133
BspCNI CTCAG 3 cut(s) 76, 800, 1000
BspDI ATCGAT 2 cut(s) 108, 2172
BspFNI CGCG 2 cut(s) 613, 1037
BspLI GGNNCC 4 cut(s) 98, 1338, 1395, 2077
BspMAI CTGCAG 3 cut(s) 1667, 1942, 2326
BspPI GGATC 4 cut(s) 561, 1542, 1939, 2028
BspT104I TTCGAA 2 cut(s) 418, 1003
BspT107I GGYRCC 1 cut(s) 1393
BspTNI GGTCTC 1 cut(s) 1096
BsrDI GCAATG 2 cut(s) 41, 474
BsrFI RCCGGY 1 cut(s) 1490
BsrI ACTGG 3 cut(s) 309, 716, 731
BssAI RCCGGY 1 cut(s) 1490
BssECI CCNNGG 3 cut(s) 1397, 1642, 1649
BssMI GATC 8 cut(s) 553, 1084, 1215, 1258, 1534, 1630, 1944, 2020
BssSI CACGAG 1 cut(s) 1871
BssT1I CCWWGG 3 cut(s) 1397, 1642, 1649
Bst2BI CACGAG 1 cut(s) 1871
Bst2UI CCWGG 1 cut(s) 182
Bst4CI ACNGT 7 cut(s) 91, 229, 660, 874, 886, 1390, 2272
Bst6I CTCTTC 1 cut(s) 1421
BstBAI YACGTR 2 cut(s) 457, 2187
BstBI TTCGAA 2 cut(s) 418, 1003
BstC8I GCNNGC 3 cut(s) 1492, 2208, 2290
BstDEI CTNAG 7 cut(s) 63, 787, 1008, 1521, 1808, 2231, 2284
BstF5I GGATG 8 cut(s) 167, 313, 433, 598, 698, 837, 1900, 2236
BstFNI CGCG 2 cut(s) 613, 1037
BstHHI GCGC 3 cut(s) 349, 615, 1151
BstKTI GATC 8 cut(s) 556, 1087, 1218, 1261, 1537, 1633, 1947, 2023
BstMAI GTCTC 6 cut(s) 288, 1096, 1758, 2028, 2041, 2250
BstMBI GATC 8 cut(s) 553, 1084, 1215, 1258, 1534, 1630, 1944, 2020
BstMCI CGRYCG 1 cut(s) 1041
BstNI CCWGG 1 cut(s) 182
BstNSI RCATGY 8 cut(s) 263, 353, 642, 1774, 1925, 2017, 2054, 2267
BstSCI CCNGG 3 cut(s) 93, 180, 1438
BstSFI CTRYAG 3 cut(s) 1663, 1938, 2322
BstSLI GKGCMC 5 cut(s) 100, 488, 811, 945, 1417
BstSNI TACGTA 2 cut(s) 457, 2187
BstUI CGCG 2 cut(s) 613, 1037
BstV1I GCAGC 6 cut(s) 1030, 1033, 1677, 2015, 2167, 2170
BstX2I RGATCY 3 cut(s) 553, 1534, 1944
BstYI RGATCY 3 cut(s) 553, 1534, 1944
Bsu15I ATCGAT 2 cut(s) 108, 2172
BsuTUI ATCGAT 2 cut(s) 108, 2172
BtrI CACGTC 1 cut(s) 1618
BtsCI GGATG 8 cut(s) 167, 313, 433, 598, 698, 837, 1900, 2236
BtsI GCAGTG 1 cut(s) 2331
BtsIMutI CAGTG 5 cut(s) 234, 879, 1326, 2277, 2331
Cac8I GCNNGC 3 cut(s) 1492, 2208, 2290
CaiI CAGNNNCTG 1 cut(s) 804
CfoI GCGC 3 cut(s) 349, 615, 1151
Cfr10I RCCGGY 1 cut(s) 1490
Cfr13I GGNCC 8 cut(s) 96, 97, 433, 564, 1337, 1493, 1646, 2105
ClaI ATCGAT 2 cut(s) 108, 2172
CseI GACGC 1 cut(s) 2045
CsiI ACCWGGT 1 cut(s) 180
Csp6I GTAC 5 cut(s) 463, 599, 1515, 1693, 2188
CspCI CAANNNNNGTGG 2 cut(s) 2199, 2234
CviQI GTAC 5 cut(s) 463, 599, 1515, 1693, 2188
DdeI CTNAG 7 cut(s) 63, 787, 1008, 1521, 1808, 2231, 2284
DpnI GATC 8 cut(s) 555, 1086, 1217, 1260, 1536, 1632, 1946, 2022
DpnII GATC 8 cut(s) 553, 1084, 1215, 1258, 1534, 1630, 1944, 2020
DriI GACNNNNNGTC 3 cut(s) 1109, 1771, 2041
EaeI YGGCCR 2 cut(s) 525, 1831
Eam1104I CTCTTC 1 cut(s) 1421
Eam1105I GACNNNNNGTC 3 cut(s) 1109, 1771, 2041
EarI CTCTTC 1 cut(s) 1421
Ecl136II GAGCTC 1 cut(s) 62
Eco105I TACGTA 2 cut(s) 457, 2187
Eco130I CCWWGG 3 cut(s) 1397, 1642, 1649
Eco24I GRGCYC 3 cut(s) 64, 100, 1328
Eco31I GGTCTC 1 cut(s) 1096
Eco47I GGWCC 4 cut(s) 433, 1337, 1646, 2105
Eco53kI GAGCTC 1 cut(s) 62
Eco57I CTGAAG 2 cut(s) 134, 562
EcoICRI GAGCTC 1 cut(s) 62
EcoO109I RGGNCCY 1 cut(s) 97
EcoRI GAATTC 1 cut(s) 1171
EcoRII CCWGG 1 cut(s) 180
EcoT14I CCWWGG 3 cut(s) 1397, 1642, 1649
EcoT22I ATGCAT 1 cut(s) 265
EcoT38I GRGCYC 3 cut(s) 64, 100, 1328
ErhI CCWWGG 3 cut(s) 1397, 1642, 1649
Esp3I CGTCTC 2 cut(s) 288, 2028
FaqI GGGAC 4 cut(s) 315, 694, 1492, 1712
FauNDI CATATG 1 cut(s) 1089
FbaI TGATCA 1 cut(s) 1258
FblI GTMKAC 2 cut(s) 201, 507
Fnu4HI GCNGC 8 cut(s) 744, 1044, 1047, 1050, 1666, 2004, 2159, 2181
FokI GGATG 8 cut(s) 174, 320, 440, 605, 705, 844, 1907, 2223
FriOI GRGCYC 3 cut(s) 64, 100, 1328
Fsp4HI GCNGC 8 cut(s) 744, 1044, 1047, 1050, 1666, 2004, 2159, 2181
FspBI CTAG 2 cut(s) 1023, 1398
FspI TGCGCA 1 cut(s) 348
GlaI GCGC 3 cut(s) 348, 614, 1150
GluI GCNGC 8 cut(s) 744, 1044, 1047, 1050, 1666, 2004, 2159, 2181
HapII CCGG 4 cut(s) 94, 1276, 1439, 1491
HgaI GACGC 1 cut(s) 2045
HhaI GCGC 3 cut(s) 349, 615, 1151
Hin6I GCGC 3 cut(s) 347, 613, 1149
HinP1I GCGC 3 cut(s) 347, 613, 1149
HincII GTYRAC 5 cut(s) 202, 508, 700, 736, 1776
HindII GTYRAC 5 cut(s) 202, 508, 700, 736, 1776
HinfI GANTC 4 cut(s) 288, 801, 1202, 1789
HpaII CCGG 4 cut(s) 94, 1276, 1439, 1491
HphI GGTGA 2 cut(s) 833, 1282
Hpy188III TCNNGA 5 cut(s) 1741, 1781, 1983, 2046, 2298
Hpy99I CGWCG 1 cut(s) 206
HpyCH4III ACNGT 7 cut(s) 91, 229, 660, 874, 886, 1390, 2272
HpyCH4IV ACGT 4 cut(s) 456, 960, 1617, 2186
HpyF3I CTNAG 7 cut(s) 63, 787, 1008, 1521, 1808, 2231, 2284
HpySE526I ACGT 4 cut(s) 456, 960, 1617, 2186
HspAI GCGC 3 cut(s) 347, 613, 1149
KroI GCCGGC 1 cut(s) 1490
KroNI GCCGGC 1 cut(s) 1492
Ksp22I TGATCA 1 cut(s) 1258
Kzo9I GATC 8 cut(s) 553, 1084, 1215, 1258, 1534, 1630, 1944, 2020
LmnI GCTCC 5 cut(s) 1356, 1564, 1654, 1963, 2099
Lsp1109I GCAGC 6 cut(s) 1030, 1033, 1677, 2015, 2167, 2170
LweI GCATC 9 cut(s) 53, 152, 272, 1477, 1885, 1984, 2245, 2270, 2301
MabI ACCWGGT 1 cut(s) 180
MaeI CTAG 2 cut(s) 1023, 1398
MaeII ACGT 4 cut(s) 456, 960, 1617, 2186
MaeIII GTNAC 7 cut(s) 329, 781, 821, 1142, 1452, 1618, 2266
MalI GATC 8 cut(s) 555, 1086, 1217, 1260, 1536, 1632, 1946, 2022
MboI GATC 8 cut(s) 553, 1084, 1215, 1258, 1534, 1630, 1944, 2020
MboII GAAGA 5 cut(s) 407, 1438, 1625, 2128, 2254
MflI RGATCY 3 cut(s) 553, 1534, 1944
MhlI GDGCHC 8 cut(s) 64, 100, 488, 811, 945, 1328, 1417, 1960
MlsI TGGCCA 2 cut(s) 527, 1833
MluCI AATT 9 cut(s) 559, 748, 977, 1079, 1171, 1192, 1434, 1835, 2191
MluNI TGGCCA 2 cut(s) 527, 1833
MlyI GAGTC 2 cut(s) 297, 810
MmeI TCCRAC 2 cut(s) 679, 1989
Mox20I TGGCCA 2 cut(s) 527, 1833
Mph1103I ATGCAT 1 cut(s) 265
MroNI GCCGGC 1 cut(s) 1490
MroXI GAANNNNTTC 3 cut(s) 999, 1171, 1788
MscI TGGCCA 2 cut(s) 527, 1833
MseI TTAA 5 cut(s) 558, 765, 1422, 1698, 2341
MslI CAYNNNNRTG 3 cut(s) 830, 1158, 2028
Msp20I TGGCCA 2 cut(s) 527, 1833
MspA1I CMGCKG 2 cut(s) 338, 1043
MspI CCGG 4 cut(s) 94, 1276, 1439, 1491
MspR9I CCNGG 3 cut(s) 95, 182, 1440
Mva1269I GAATGC 1 cut(s) 1617
MvaI CCWGG 1 cut(s) 182
MvnI CGCG 2 cut(s) 613, 1037
NaeI GCCGGC 1 cut(s) 1492
NciI CCSGG 2 cut(s) 95, 1440
NdeI CATATG 1 cut(s) 1089
NdeII GATC 8 cut(s) 553, 1084, 1215, 1258, 1534, 1630, 1944, 2020
NgoMIV GCCGGC 1 cut(s) 1490
NlaIV GGNNCC 4 cut(s) 98, 1338, 1395, 2077
NmuCI GTSAC 4 cut(s) 821, 1452, 1618, 2266
NsbI TGCGCA 1 cut(s) 348
NsiI ATGCAT 1 cut(s) 265
NspI RCATGY 8 cut(s) 263, 353, 642, 1774, 1925, 2017, 2054, 2267
NspV TTCGAA 2 cut(s) 418, 1003
PciI ACATGT 1 cut(s) 1770
PctI GAATGC 1 cut(s) 1617
PdiI GCCGGC 1 cut(s) 1492
PdmI GAANNNNTTC 3 cut(s) 999, 1171, 1788
PfeI GAWTC 2 cut(s) 1202, 1789
PkrI GCNGC 8 cut(s) 745, 1045, 1048, 1051, 1667, 2005, 2160, 2182
PleI GAGTC 2 cut(s) 296, 809
PpsI GAGTC 2 cut(s) 296, 809
Ppu21I YACGTR 2 cut(s) 457, 2187
PscI ACATGT 1 cut(s) 1770
PsiI TTATAA 1 cut(s) 1676
Psp124BI GAGCTC 1 cut(s) 64
Psp6I CCWGG 1 cut(s) 180
PspGI CCWGG 1 cut(s) 180
PspN4I GGNNCC 4 cut(s) 98, 1338, 1395, 2077
PspOMI GGGCCC 1 cut(s) 96
PspPI GGNCC 8 cut(s) 96, 97, 433, 564, 1337, 1493, 1646, 2105
PstI CTGCAG 3 cut(s) 1667, 1942, 2326
PstNI CAGNNNCTG 1 cut(s) 804
PsuI RGATCY 3 cut(s) 553, 1534, 1944
RsaI GTAC 5 cut(s) 464, 600, 1516, 1694, 2189
RsaNI GTAC 5 cut(s) 463, 599, 1515, 1693, 2188
RseI CAYNNNNRTG 3 cut(s) 830, 1158, 2028
SacI GAGCTC 1 cut(s) 64
SalI GTCGAC 2 cut(s) 200, 506
SaqAI TTAA 5 cut(s) 558, 765, 1422, 1698, 2341
SatI GCNGC 8 cut(s) 744, 1044, 1047, 1050, 1666, 2004, 2159, 2181
Sau3AI GATC 8 cut(s) 553, 1084, 1215, 1258, 1534, 1630, 1944, 2020
Sau96I GGNCC 8 cut(s) 96, 97, 433, 564, 1337, 1493, 1646, 2105
SchI GAGTC 2 cut(s) 297, 810
ScrFI CCNGG 3 cut(s) 95, 182, 1440
SduI GDGCHC 8 cut(s) 64, 100, 488, 811, 945, 1328, 1417, 1960
SexAI ACCWGGT 1 cut(s) 180
SfaNI GCATC 9 cut(s) 53, 152, 272, 1477, 1885, 1984, 2245, 2270, 2301
SfcI CTRYAG 3 cut(s) 1663, 1938, 2322
SfuI TTCGAA 2 cut(s) 418, 1003
SinI GGWCC 4 cut(s) 433, 1337, 1646, 2105
SmiMI CAYNNNNRTG 3 cut(s) 830, 1158, 2028
SmlI CTYRAG 1 cut(s) 1779
SmoI CTYRAG 1 cut(s) 1779
SnaBI TACGTA 2 cut(s) 457, 2187
Sse9I AATT 9 cut(s) 559, 748, 977, 1079, 1171, 1192, 1434, 1835, 2191
SsiI CCGC 7 cut(s) 338, 633, 744, 1035, 1041, 1050, 2133
SspI AATATT 1 cut(s) 441
SspMI CTAG 2 cut(s) 1023, 1398
SstI GAGCTC 1 cut(s) 64
StyD4I CCNGG 3 cut(s) 93, 180, 1438
StyI CCWWGG 3 cut(s) 1397, 1642, 1649
TaaI ACNGT 7 cut(s) 91, 229, 660, 874, 886, 1390, 2272
TaiI ACGT 4 cut(s) 459, 963, 1620, 2189
TasI AATT 9 cut(s) 559, 748, 977, 1079, 1171, 1192, 1434, 1835, 2191
TatI WGTACW 1 cut(s) 598
TauI GCSGC 2 cut(s) 746, 1052
TfiI GAWTC 2 cut(s) 1202, 1789
Tru1I TTAA 5 cut(s) 558, 765, 1422, 1698, 2341
Tru9I TTAA 5 cut(s) 558, 765, 1422, 1698, 2341
TscAI CASTG 5 cut(s) 234, 879, 1326, 2277, 2331
TseFI GTSAC 4 cut(s) 821, 1452, 1618, 2266
TseI GCWGC 6 cut(s) 1043, 1046, 1665, 2003, 2158, 2180
Tsp45I GTSAC 4 cut(s) 821, 1452, 1618, 2266
TspDTI ATGAA 6 cut(s) 70, 237, 848, 873, 965, 1816
TspGWI ACGGA 1 cut(s) 1490
TspRI CASTG 5 cut(s) 234, 879, 1326, 2277, 2331
VneI GTGCAC 2 cut(s) 941, 1413
VpaK11BI GGWCC 4 cut(s) 433, 1337, 1646, 2105
XapI RAATTY 2 cut(s) 748, 1171
XceI RCATGY 8 cut(s) 263, 353, 642, 1774, 1925, 2017, 2054, 2267
XcmI CCANNNNNNNNNTGG 1 cut(s) 723
XmaJI CCTAGG 1 cut(s) 1397
XmiI GTMKAC 2 cut(s) 201, 507
XmnI GAANNNNTTC 3 cut(s) 999, 1171, 1788
XspI CTAG 2 cut(s) 1023, 1398
Zsp2I ATGCAT 1 cut(s) 265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.