MD07G1028000.v1.1

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000035
Physical Location & Seq
Reverse (-)
2336488 .. 2339520
3033 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1028000.v1.1.491

Sequence Viewer

Length: 2922 bp
ATGGCGGGGGCAATTGTGGGCGGCGCTTTTCTCTCTTCCTTTCTCAACGTCTTGTTTGATAGGCTGGCCTCCCGTCCCGTCGTCGACTTCTTCCGTGGACAGAAGATCAACAGCGGACTGCTCAATAAGTTGAAGATTAAGCTGCTTTCAGTTAATTCGGTGCTCGATGATGCGGAGGAGAAGCAATTCAAAAACCCAGCTGTGAGGGAGTGGCTCGACGAACTCAAAGATGCTCTGTATGCTGCAGACGACCTGCTTGATGAGATCAAGGCTGAAGCTCTGCGGCGAAAGCTCGAAGGCGATGAATCCAGCAGCAGCAAAAGTCAGGTACGGATCTCCAGTTCCGATGAATTTGACAAAACAATGGAACCCAAGATTGTAAAAATGCTTGAGAGGTTAGAATTTATTGTAAATGAGAAAGATGTGCTTGGTTTGAAAGAGGGTGTTCCAAATCAGCGATCACAATCCAGATTATCTACCACTTCTTTGGTGGAAGAGGACGGTGTGTACGGTAGGGATGAGGACAAGGAGGCCATTGTGAAGCTATTGCTATCCGATGATGCGAATGGCAGTAAGTTGAGTGTGATTCCGGTTGTGGGAATGGGTGGCATCGGAAAGACCACCCTTGCGCAGCTCGTATACAACGATGCTAGAGTGAAGCAACATTTTGACTTGCAAGCATGGGTTTATGTTTCAGAAGAATTTGATGTTGTTAGAATAACTCAAGGAGGCTTTGACAATTATATTCACTGGGATGCGTTGCGGTGTTCTTTTGAGTCTGTCGCACACGGAAGTAAGATCATTGTGACTACACGTAACCAACGTGTTGCATCAATGATGGGTACTGTCACAACTTATCATCTAAACCACATATCTGAAAAAGATTGTGGGTTGTTATTTTCCAAGCATGCCTTCGGCAGGATAAACTCAGCTGTGCATCCAACTCTAGAAGAAATCAGTAGCGGAATAATTAAGAAGTGTAAAGGTCTTCCTTTAGCTGCAAAGTCTCTTGGGGGTTTGTTGTGCTGTAAACAAAGTTACAAGGAGTGGGATGAAATATTGAAGAGTGAAATATGGGACTTGCAAGATGATACCATTCTTCCAGCTTTGAGATTAAGTTATCATCATCTTCCTTCTCATCTAAAACGATGTTTTTCCTATTGTTCGATTTTCCCCAAAGGTTACAAATTTGGAAAATCAGAATTAGTTTTGTTGTGGATGGCTGAAGGTTTTTTGTCACCCCAAAGAAAGAAAATGATGGAAGACGTTGGAGGGGAGTACTTTGATGATTTAATATCGAGGTCATTTTTTCAGCATTCAGATGATGAGTCTGATGACCAATCACTTTTTACCATGCACGACCTTATCAATGATTTAGCAAAGTTTGTATCAAGAGAATTTTGTTTTAGGTTGGATGATAGTGACTCATTCAATAATGTTTCAAGCAAGACTCGTCATCTCTCATACACTCTGAATAACCCCTTCAAAAGACTTGGTGTAGGTGTTCATGCGACTGCAAAGGAGGAGATTTCTGGTTTTGATAAATTTGAAGCTTTATGCAAAGCCAAGTATTTGCGCACCTTCCTAGCAATACAACCTAAACCATTATCGAGAATTTTCTGTTTCAACAAGGTACCTCATGATCTCTTACAAACTGTGTATTGTTTGAGAGTGCTCTCTTTATCTCATTGTGACAACAAGGAGTTGCCTGATTGGATTGGCAATTTAAAACACCTAAGGTATATAGACTTGTCATACGGTGGATTTGAAAAGTTACCTGAAACAATATGTACTTTGCATAATCTACAAACGTTGCTCTTGTTCAATTGTTATGAACTTGCTCAGTTGCCAATCAACTTGGGGAGACTCATCAACTTGCGCCATCTTGATGTTAGAAGCACTAATCTAAAAGAGATGCCACCACATATGGATAAGTTGAAGGATCTCCACACATTGAGTGACTTTGTTGTTGGCAAACAAACTGCACCTAGAATTGTAGTGTTGAAAGAGCTTCAGATAGTAGGTACACTTGCTATTTCAGGGCTTCATAATATTGTGAATTATGAGGATGCTTTTGTAGCCAATATGAGAAATAAGCACCTTGACGGACTAGCTTTGACATGGGGAGCTGAGACCGATGATTCACAAAAAGATAGAGAGGTGCTTAATAATCTCCATCCTCATACACACCTGAAAGCCCTCAGTCTTAAGTTTTATGGGGGTACAAGATTTCCTGATTGGTTAGGAGATCATTCTTTTTCTAATTTGGTTTCTCTTCAACTCGAAGATTGTAAACATTGTTGCTCCTTGCCACCACTGGGTCAGCTACCCTCCCTCGTCACGCTTTATGTTTGTGGGTTAAATGAAGTGGAGACCATAGGGCCTGAGTTTTATGGTAATGGTGTTTCTGTTGTTATGCCATTTAGATCTCTATCAGTTTTATTCTTCAAAGATATGTTGGGATGGCAAGAGTGGTCTCATTTCGGAAGTAGCCAAGAAGGTGGAGCTTTTCCTCATCTTTGTCAGCTCTATCTGACAAATTGTCCCAAGCTAATAGAGAAATTACCTGAGTTTCTTCCATCCTTGATTACACTTGAGTTAAGGAGATGCGAGCAACTTCTGGGTGCACTTCCAAGAACTCGGGCCATTGTGGAGATTCCTTCTGTGAAAGACAACCTTTGTCTTGAAGAGAAAACTAGCGGTACTAACTCATCTCTTACTCCATTTCCTTGTGACGGTCTGGCTGATGCTTTAAGAGTTCTTAAAATAAAAAATTGTTGGAACTTATCTCCACTAAATCACTGCTATGCATTCCTTCAAACATTGAAGATAAAGAGTAGCTGTGATTCAACCAAGTCTATCCCGCTGGACTACTTCCCAAAGCCCTATAATCCCGTCCCTCAGTTTCTTGAGAATATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

974

Amino Acids

109.88

Weight (kDa)

6.3

Isoelectric Point (pI)

40.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 10 - 104 6e-20 Rx N-terminal domain
NB-ARC PF00931 173 - 241 5.1e-13 NB-ARC domain
WHD_DRP PF23559 390 - 460 9.9e-28 Disease resistance protein Winged helix domain
LRR_14 PF23598 550 - 845 3.8e-27 Leucine-rich repeat region
LRR_Zer-1 PF25013 574 - 652 6.1e-06 Zer-1-like, Leucine-rich repeats
LRR_R13L1-DRL21 PF25019 667 - 785 5.1e-33 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000035)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14470 AT3G14470
fragaria_vesca FvH4_1g01220 FvH4_1g01230 FvH4_3g24551 FvH4_3g24552 FvH4_4g29310 FvH4_4g29930 FvH4_4g29930 FvH4_4g29930 FvH4_4g29930 FvH4_4g29930 FvH4_4g29930 FvH4_4g29930 FvH4_4g29930 FvH4_4g29930 FvH4_6g39860 FvH4_6g39861 FvH4_7g11380
malus_domestica MD00G1072900.v1.1 MD00G1073100.v1.1 MD00G1120600.v1.1 MD00G1120900.v1.1 MD00G1121000.v1.1 MD00G1121200.v1.1 MD00G1152300.v1.1 MD00G1203600.v1.1 MD00G1203700.v1.1 MD02G1250900.v1.1 MD02G1251900.v1.1 MD02G1252800.v1.1 MD02G1277100.v1.1 MD02G1277200.v1.1 MD02G1277400.v1.1 MD02G1286100.v1.1 MD02G1286200.v1.1 MD02G1286900.v1.1 MD03G1006200.v1.1 MD03G1015300.v1.1 MD03G1015500.v1.1 MD03G1015900.v1.1 MD03G1016000.v1.1 MD03G1024900.v1.1 MD03G1025000.v1.1 MD03G1025500.v1.1 MD03G1026000.v1.1 MD03G1026500.v1.1 MD03G1031800.v1.1 MD03G1072200.v1.1 MD07G1028000.v1.1 MD07G1028300.v1.1 MD07G1029400.v1.1 MD07G1029500.v1.1 MD07G1040300.v1.1 MD07G1044500.v1.1 MD07G1045100.v1.1 MD07G1045500.v1.1 MD07G1050700.v1.1 MD07G1050900.v1.1 MD07G1055800.v1.1 MD07G1056200.v1.1 MD07G1056600.v1.1 MD07G1057100.v1.1 MD07G1057200.v1.1 MD08G1056500.v1.1 MD08G1056600.v1.1 MD08G1056700.v1.1 MD08G1056800.v1.1 MD08G1128800.v1.1 MD08G1129500.v1.1 MD08G1130000.v1.1 MD11G1066700.v1.1 MD11G1066800.v1.1 MD11G1066900.v1.1 MD11G1067000.v1.1 MD11G1067500.v1.1 MD11G1069800.v1.1 MD11G1070400.v1.1 MD11G1071100.v1.1 MD11G1071200.v1.1 MD11G1077200.v1.1 MD11G1077700.v1.1 MD11G1077900.v1.1 MD11G1078100.v1.1 MD11G1078300.v1.1 MD11G1078500.v1.1 MD11G1135200.v1.1 MD11G1135400.v1.1 MD11G1152900.v1.1 MD11G1153000.v1.1 MD17G1056800.v1.1
prunus_persica Prupe.2G037800_v2.0.a1 Prupe.2G037800_v2.0.a1 Prupe.2G037800_v2.0.a1 Prupe.2G037800_v2.0.a1 Prupe.2G040500_v2.0.a1 Prupe.2G040700_v2.0.a1 Prupe.2G043000_v2.0.a1 Prupe.2G043100_v2.0.a1 Prupe.2G043200_v2.0.a1 Prupe.2G046000_v2.0.a1 Prupe.2G046300_v2.0.a1 Prupe.2G046400_v2.0.a1 Prupe.2G046400_v2.0.a1 Prupe.2G046700_v2.0.a1 Prupe.2G046900_v2.0.a1 Prupe.2G051100_v2.0.a1 Prupe.2G052200_v2.0.a1 Prupe.2G053600_v2.0.a1 Prupe.2G053600_v2.0.a1 Prupe.2G053600_v2.0.a1 Prupe.2G053600_v2.0.a1 Prupe.2G053700_v2.0.a1 Prupe.2G053800_v2.0.a1 Prupe.2G053900_v2.0.a1 Prupe.2G054000_v2.0.a1 Prupe.2G054100_v2.0.a1 Prupe.2G054200_v2.0.a1 Prupe.2G054300_v2.0.a1 Prupe.2G054300_v2.0.a1 Prupe.2G054300_v2.0.a1 Prupe.2G054300_v2.0.a1 Prupe.2G054500_v2.0.a1 Prupe.2G054700_v2.0.a1 Prupe.4G286000_v2.0.a1 Prupe.4G286000_v2.0.a1 Prupe.4G286000_v2.0.a1 Prupe.6G129800_v2.0.a1 Prupe.6G130000_v2.0.a1 Prupe.6G130000_v2.0.a1
pyrus_communis pycom02g23740 pycom02g23750 pycom02g24320 pycom03g00620 pycom03g01280 pycom03g01320 pycom03g01350 pycom03g01360 pycom03g01370 pycom03g01380 pycom03g01390 pycom03g02160 pycom03g02170 pycom03g05850 pycom03g09810 pycom07g02120 pycom07g02790 pycom07g02800 pycom07g03210 pycom07g03660 pycom07g03670 pycom07g04350 pycom08g10780 pycom08g10840 pycom08g10910 pycom11g05650 pycom11g05670 pycom11g05800 pycom11g05880 pycom11g05890 pycom11g05940 pycom11g06490 pycom11g06500 pycom11g06520 pycom11g06560 pycom11g06570 pycom11g11230 pycom11g13650 pycom17g05700
rosa_chinensis RchiOBHm_Chr1g0318341 RchiOBHm_Chr1g0318901 RchiOBHm_Chr1g0318931 RchiOBHm_Chr1g0327301 RchiOBHm_Chr1g0328491 RchiOBHm_Chr1g0336761 RchiOBHm_Chr1g0336831 RchiOBHm_Chr1g0340721 RchiOBHm_Chr1g0342461 RchiOBHm_Chr1g0342471 RchiOBHm_Chr1g0342481 RchiOBHm_Chr1g0346131 RchiOBHm_Chr1g0346191 RchiOBHm_Chr1g0346211 RchiOBHm_Chr1g0346241 RchiOBHm_Chr1g0346261 RchiOBHm_Chr1g0346281 RchiOBHm_Chr1g0346461 RchiOBHm_Chr1g0346471 RchiOBHm_Chr1g0346481 RchiOBHm_Chr1g0346501 RchiOBHm_Chr1g0347691 RchiOBHm_Chr1g0347701 RchiOBHm_Chr1g0347751 RchiOBHm_Chr1g0347791 RchiOBHm_Chr1g0347971 RchiOBHm_Chr1g0349661 RchiOBHm_Chr1g0349741 RchiOBHm_Chr1g0357561 RchiOBHm_Chr3g0492601 RchiOBHm_Chr5g0039181 RchiOBHm_Chr5g0039231 RchiOBHm_Chr5g0039241 RchiOBHm_Chr5g0039251 RchiOBHm_Chr5g0039531 RchiOBHm_Chr5g0044001 RchiOBHm_Chr5g0044571 RchiOBHm_Chr5g0044611 RchiOBHm_Chr5g0044631 RchiOBHm_Chr5g0044641 RchiOBHm_Chr5g0044651 RchiOBHm_Chr5g0044661 RchiOBHm_Chr5g0044681 RchiOBHm_Chr5g0045221 RchiOBHm_Chr5g0045901 RchiOBHm_Chr5g0045991 RchiOBHm_Chr5g0046001 RchiOBHm_Chr5g0046011
rosa_laevigata RLG00000028715 RLG00000028718 RLG00000029034 RLG00000029926 RLG00000030516 RLG00000034285 RLG00000034288
rosa_multiflora Rmu_co7987576.1_g000001 Rmu_co8028038.1_g000001 Rmu_co8104858.1_g000001 Rmu_co8185828.1_g000001 Rmu_co8227651.1_g000001 Rmu_co8235061.1_g000001 Rmu_co8246845.1_g000001 Rmu_co8254315.1_g000001 Rmu_co8284899.1_g000001 Rmu_co8401461.1_g000001 Rmu_co8475625.1_g000001 Rmu_co8502681.1_g000001 Rmu_sc0000250.1_g000005 Rmu_sc0000492.1_g000001 Rmu_sc0000533.1_g000004 Rmu_sc0000795.1_g000114 Rmu_sc0000831.1_g000039 Rmu_sc0000907.1_g000014 Rmu_sc0000907.1_g000034 Rmu_sc0001035.1_g000007 Rmu_sc0001035.1_g000011 Rmu_sc0001035.1_g000012 Rmu_sc0001099.1_g000003 Rmu_sc0001157.1_g000008 Rmu_sc0001212.1_g000016 Rmu_sc0001212.1_g000017 Rmu_sc0001304.1_g000027 Rmu_sc0001304.1_g000033 Rmu_sc0001773.1_g000019 Rmu_sc0001836.1_g000069 Rmu_sc0001856.1_g000004 Rmu_sc0002286.1_g000003 Rmu_sc0002286.1_g000004 Rmu_sc0002286.1_g000005 Rmu_sc0002286.1_g000007 Rmu_sc0002286.1_g000015 Rmu_sc0002495.1_g000025 Rmu_sc0002545.1_g000008 Rmu_sc0002545.1_g000009 Rmu_sc0002662.1_g000009 Rmu_sc0003363.1_g000019 Rmu_sc0003363.1_g000022 Rmu_sc0003600.1_g000057 Rmu_sc0003809.1_g000036 Rmu_sc0003809.1_g000037 Rmu_sc0005008.1_g000026 Rmu_sc0005008.1_g000028 Rmu_sc0005028.1_g000001 Rmu_sc0005144.1_g000022 Rmu_sc0005167.1_g000004 Rmu_sc0005626.1_g000005 Rmu_sc0005626.1_g000018 Rmu_sc0006228.1_g000011 Rmu_sc0006609.1_g000007 Rmu_sc0006609.1_g000009 Rmu_sc0006609.1_g000015 Rmu_sc0006609.1_g000016 Rmu_sc0006609.1_g000017 Rmu_sc0006609.1_g000018 Rmu_sc0007060.1_g000003 Rmu_sc0007318.1_g000011 Rmu_sc0007992.1_g000002 Rmu_sc0009172.1_g000001 Rmu_sc0010151.1_g000010 Rmu_sc0010151.1_g000011 Rmu_sc0011778.1_g000001 Rmu_sc0011778.1_g000005 Rmu_sc0011778.1_g000009 Rmu_sc0012293.1_g000001 Rmu_sc0012293.1_g000002 Rmu_sc0016606.1_g000001 Rmu_sc0016836.1_g000001 Rmu_sc0021084.1_g000008 Rmu_sc0021449.1_g000001 Rmu_sc0026523.1_g000001 Rmu_sc0027133.1_g000003 Rmu_sc0038674.1_g000001 Rmu_ssc0000255.1_g000053 Rmu_ssc0000255.1_g000063 Rmu_ssc0000255.1_g000064 Rmu_ssc0000263.1_g000004 Rmu_ssc0000400.1_g000027
rosa_roxburghii Rroxscaffold_1G00035290 Rroxscaffold_1G00036350 Rroxscaffold_1G00036360 Rroxscaffold_1G00036400 Rroxscaffold_1G00036460 Rroxscaffold_1G00037000 Rroxscaffold_1G00040440 Rroxscaffold_1G00041890 Rroxscaffold_1G00041920 Rroxscaffold_4G00307460 Rroxscaffold_4G00307500 Rroxscaffold_4G00308500 Rroxscaffold_4G00308510 Rroxscaffold_4G00308770 Rroxscaffold_4G00313020 Rroxscaffold_4G00321580 Rroxscaffold_4G00323030 Rroxscaffold_4G00326850 Rroxscaffold_4G00328770 Rroxscaffold_4G00328780 Rroxscaffold_4G00329310 Rroxscaffold_5G00352110
rosa_rugosa Rorug01G0020700 Rorug01G0023000 Rorug01G0023100 Rorug01G0023200 Rorug01G0023300 Rorug01G0023400 Rorug01G0024500 Rorug01G0025100 Rorug01G0025200 Rorug01G0025400 Rorug01G0025400 Rorug01G0064700 Rorug01G0065300 Rorug01G0127600 Rorug01G0128000 Rorug01G0148000.1 Rorug01G0150500.1 Rorug01G0161600.1 Rorug01G0161800.1 Rorug01G0163600.1 Rorug01G0178300 Rorug01G0178500 Rorug01G0178700 Rorug01G0179900 Rorug01G0180200 Rorug01G0180300 Rorug01G0180400 Rorug01G0189000 Rorug01G0189100 Rorug01G0190500 Rorug01G0204800 Rorug05G0177200 Rorug05G0210300 Rorug05G0210300 Rorug05G0210300 Rorug05G0215400 Rorug05G0215400 Rorug05G0215400 Rorug05G0215400 Rorug05G0215500 Rorug05G0215600 Rorug05G0215800 Rorug05G0215900 Rorug05G0215900 Rorug05G0216000 Rorug05G0216200 Rorug05G0216300 Rorug05G0221900 Rorug07G0276700
rosa_samantha Rh1BG028400 Rh1BG033500 Rh1BG065100 Rh1BG073100 Rh1BG073200 Rh1BG117000 Rh1BG164000 Rh1BG164200 Rh1BG172100 Rh1BG173900 Rh1BG174100 Rh1BG188400 Rh1CG036000 Rh1CG036500 Rh1CG089900 Rh1CG140200 Rh1CG164700 Rh1CG182600 Rh1CG182900 Rh1CG183400 Rh1CG191300 Rh1CG192000 Rh1CG207700 Rh1DG033800 Rh1DG033900 Rh1DG096000 Rh5AG299900 Rh5AG304500 Rh5AG307100 Rh5AG307500 Rh5CG301600 Rh5CG329800 Rh5CG333800 Rh5DG311400 Rh5DG316300 Rh5DG316400 Rh5DG316700 Rh5DG316800 Rh5DG316900 Rh5DG326100 Rh7AG431900 Rh7CG451000
rosa_wichuraiana Rw0G007840 Rw0G009850 Rw0G013000 Rw0G015090 Rw0G021190 Rw1G002610 Rw1G004320 Rw1G006520 Rw1G007250 Rw1G012020 Rw1G013830 Rw1G014770 Rw1G016300 Rw1G016600 Rw1G016650 Rw1G017290 Rw1G017420 Rw1G017630 Rw1G017650 Rw1G017660 Rw1G017690 Rw1G017700 Rw1G018960 Rw5G024660 Rw5G024700 Rw5G024720 Rw5G024740 Rw5G024980 Rw5G027710 Rw5G027720 Rw5G027730 Rw5G027740 Rw5G027750 Rw5G028130 Rw5G028740 Rw5G028750 Rw5G028790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 630, 1577
Acc36I ACCTGC 1 cut(s) 261
Acc65I GGTACC 1 cut(s) 1633
AccB1I GGYRCC 1 cut(s) 1633
AccI GTMKAC 2 cut(s) 84, 639
AciI CCGC 9 cut(s) 5, 21, 114, 173, 283, 763, 963, 2700, 2864
AclI AACGTT 1 cut(s) 1811
AclWI GGATC 2 cut(s) 341, 1950
AcsI RAATTY 7 cut(s) 350, 401, 701, 1187, 1397, 1544, 1614
AcuI CTGAAG 3 cut(s) 294, 1245, 1997
AfaI GTAC 9 cut(s) 330, 509, 844, 1280, 1635, 1792, 2026, 2224, 2704
AfiI CCNNNNNNNGG 4 cut(s) 596, 918, 2318, 2735
AflII CTTAAG 1 cut(s) 2207
AflIII ACRYGT 2 cut(s) 812, 823
AhdI GACNNNNNGTC 1 cut(s) 2541
AjuI GAANNNNNNNTTGG 2 cut(s) 896, 928
AloI GAACNNNNNNTCC 2 cut(s) 325, 357
Alw21I GWGCWC 3 cut(s) 165, 1677, 2629
Alw26I GTCTC 5 cut(s) 1011, 1858, 2126, 2366, 2481
Alw44I GTGCAC 1 cut(s) 2625
AlwI GGATC 2 cut(s) 341, 1950
Ama87I CYCGRG 1 cut(s) 2640
AoxI GGCC 4 cut(s) 66, 531, 2381, 2643
ApaLI GTGCAC 1 cut(s) 2625
ApeKI GCWGC 6 cut(s) 142, 242, 312, 315, 631, 998
ApoI RAATTY 7 cut(s) 350, 401, 701, 1187, 1397, 1544, 1614
Asp700I GAANNNNTTC 1 cut(s) 185
Asp718I GGTACC 1 cut(s) 1633
AspLEI GCGC 4 cut(s) 26, 631, 1578, 1881
AspS9I GGNCC 2 cut(s) 2381, 2643
AsuHPI GGTGA 1 cut(s) 1230
AvaI CYCGRG 1 cut(s) 2640
AxyI CCTNAGG 1 cut(s) 1736
BaeGI GKGCMC 1 cut(s) 2629
BanI GGYRCC 1 cut(s) 1633
BbsI GAAGAC 2 cut(s) 980, 1269
Bbv12I GWGCWC 3 cut(s) 165, 1677, 2629
BbvI GCAGC 6 cut(s) 129, 229, 324, 327, 643, 985
BccI CCATC 7 cut(s) 832, 1213, 1252, 1890, 2184, 2457, 2587
BcoDI GTCTC 5 cut(s) 1011, 1858, 2126, 2366, 2481
BfaI CTAG 6 cut(s) 651, 947, 1586, 1989, 2111, 2697
BfmI CTRYAG 1 cut(s) 243
BfoI RGCGCY 1 cut(s) 27
BfrI CTTAAG 1 cut(s) 2207
BfuAI ACCTGC 1 cut(s) 261
BglII AGATCT 1 cut(s) 2426
BisI GCNGC 8 cut(s) 22, 143, 243, 284, 313, 316, 632, 999
BlsI GCNGC 8 cut(s) 23, 144, 244, 285, 314, 317, 633, 1000
BmcAI AGTACT 1 cut(s) 1280
BmeRI GACNNNNNGTC 1 cut(s) 2541
BmeT110I CYCGRG 1 cut(s) 2640
BmgT120I GGNCC 2 cut(s) 2381, 2643
BmiI GGNNCC 2 cut(s) 369, 1635
BmrI ACTGGG 2 cut(s) 760, 2327
BmuI ACTGGG 2 cut(s) 760, 2327
BpiI GAAGAC 2 cut(s) 980, 1269
BpmI CTGGAG 1 cut(s) 322
BpuEI CTTGAG 3 cut(s) 410, 708, 2615
BsaAI YACGTR 1 cut(s) 815
BsaBI GATNNNNATC 2 cut(s) 463, 2431
BsaI GGTCTC 3 cut(s) 2126, 2366, 2481
BsaJI CCNNGG 1 cut(s) 94
BsaWI WCCGGW 1 cut(s) 589
Bsc4I CCNNNNNNNGG 4 cut(s) 596, 918, 2318, 2735
Bse1I ACTGG 3 cut(s) 339, 755, 2322
Bse21I CCTNAGG 1 cut(s) 1736
Bse8I GATNNNNATC 2 cut(s) 463, 2431
BseDI CCNNGG 1 cut(s) 94
BseJI GATNNNNATC 2 cut(s) 463, 2431
BseLI CCNNNNNNNGG 4 cut(s) 596, 918, 2318, 2735
BseMII CTCAG 7 cut(s) 942, 1856, 2121, 2215, 2376, 2559, 2915
BseNI ACTGG 3 cut(s) 339, 755, 2322
BseRI GAGGAG 2 cut(s) 191, 1538
BseSI GKGCMC 1 cut(s) 2629
BseXI GCAGC 6 cut(s) 129, 229, 324, 327, 643, 985
BseYI CCCAGC 1 cut(s) 196
BsgI GTGCAG 1 cut(s) 1968
BshFI GGCC 4 cut(s) 68, 533, 2383, 2645
BshNI GGYRCC 1 cut(s) 1633
BsiHKAI GWGCWC 3 cut(s) 165, 1677, 2629
BsiHKCI CYCGRG 1 cut(s) 2640
BsiSI CCGG 1 cut(s) 590
BslFI GGGAC 4 cut(s) 60, 1091, 2529, 2882
BslI CCNNNNNNNGG 4 cut(s) 596, 918, 2318, 2735
BsmAI GTCTC 5 cut(s) 1011, 1858, 2126, 2366, 2481
BsmFI GGGAC 4 cut(s) 60, 1091, 2529, 2882
BsmI GAATGC 2 cut(s) 1315, 2810
BsnI GGCC 4 cut(s) 68, 533, 2383, 2645
Bso31I GGTCTC 3 cut(s) 2126, 2366, 2481
BsoBI CYCGRG 1 cut(s) 2640
Bsp1286I GDGCHC 3 cut(s) 165, 1677, 2629
Bsp143I GATC 9 cut(s) 105, 264, 333, 458, 798, 1642, 1942, 2248, 2426
BspACI CCGC 9 cut(s) 5, 21, 114, 173, 283, 763, 963, 2700, 2864
BspANI GGCC 4 cut(s) 68, 533, 2383, 2645
BspCNI CTCAG 7 cut(s) 941, 1855, 2122, 2214, 2377, 2560, 2914
BspHI TCATGA 1 cut(s) 1639
BspLI GGNNCC 2 cut(s) 369, 1635
BspMAI CTGCAG 1 cut(s) 247
BspMI ACCTGC 1 cut(s) 261
BspPI GGATC 2 cut(s) 341, 1950
BspT107I GGYRCC 1 cut(s) 1633
BspTI CTTAAG 1 cut(s) 2207
BspTNI GGTCTC 3 cut(s) 2126, 2366, 2481
BsrI ACTGG 3 cut(s) 339, 755, 2322
BssECI CCNNGG 1 cut(s) 94
BssMI GATC 9 cut(s) 105, 264, 333, 458, 798, 1642, 1942, 2248, 2426
BssNAI GTATAC 1 cut(s) 640
Bst1107I GTATAC 1 cut(s) 640
Bst4CI ACNGT 6 cut(s) 503, 512, 847, 1657, 1760, 2738
Bst6I CTCTTC 5 cut(s) 40, 489, 1058, 2280, 2682
BstAFI CTTAAG 1 cut(s) 2207
BstBAI YACGTR 1 cut(s) 815
BstC8I GCNNGC 4 cut(s) 66, 678, 909, 2612
BstDEI CTNAG 8 cut(s) 928, 1736, 1842, 2130, 2201, 2385, 2568, 2901
BstDSI CCRYGG 1 cut(s) 94
BstENI CCTNNNNNAGG 1 cut(s) 916
BstH2I RGCGCY 1 cut(s) 27
BstHHI GCGC 4 cut(s) 26, 631, 1578, 1881
BstKTI GATC 9 cut(s) 108, 267, 336, 461, 801, 1645, 1945, 2251, 2429
BstMAI GTCTC 5 cut(s) 1011, 1858, 2126, 2366, 2481
BstMBI GATC 9 cut(s) 105, 264, 333, 458, 798, 1642, 1942, 2248, 2426
BstMWI GCNNNNNNNGC 3 cut(s) 239, 289, 2078
BstNSI RCATGY 1 cut(s) 911
BstSFI CTRYAG 1 cut(s) 243
BstSLI GKGCMC 1 cut(s) 2629
BstV1I GCAGC 6 cut(s) 129, 229, 324, 327, 643, 985
BstV2I GAAGAC 2 cut(s) 980, 1269
BstX2I RGATCY 3 cut(s) 333, 1942, 2426
BstXI CCANNNNNNTGG 2 cut(s) 487, 2501
BstYI RGATCY 3 cut(s) 333, 1942, 2426
BstZ17I GTATAC 1 cut(s) 640
Bsu36I CCTNAGG 1 cut(s) 1736
BsuRI GGCC 4 cut(s) 68, 533, 2383, 2645
BtgI CCRYGG 1 cut(s) 94
BtgZI GCGATG 1 cut(s) 315
BtsI GCAGTG 1 cut(s) 2800
BtsIMutI CAGTG 3 cut(s) 748, 2315, 2800
BveI ACCTGC 1 cut(s) 261
Cac8I GCNNGC 4 cut(s) 66, 678, 909, 2612
CciI TCATGA 1 cut(s) 1639
CfoI GCGC 4 cut(s) 26, 631, 1578, 1881
Cfr13I GGNCC 2 cut(s) 2381, 2643
Csp6I GTAC 9 cut(s) 329, 508, 843, 1279, 1634, 1791, 2025, 2223, 2703
CviAII CATG 6 cut(s) 681, 908, 1354, 1508, 1640, 2121
CviQI GTAC 9 cut(s) 329, 508, 843, 1279, 1634, 1791, 2025, 2223, 2703
DdeI CTNAG 8 cut(s) 928, 1736, 1842, 2130, 2201, 2385, 2568, 2901
DpnI GATC 9 cut(s) 107, 266, 335, 460, 800, 1644, 1944, 2250, 2428
DpnII GATC 9 cut(s) 105, 264, 333, 458, 798, 1642, 1942, 2248, 2426
DraI TTTAAA 1 cut(s) 1728
DriI GACNNNNNGTC 1 cut(s) 2541
Eam1104I CTCTTC 5 cut(s) 40, 489, 1058, 2280, 2682
Eam1105I GACNNNNNGTC 1 cut(s) 2541
EarI CTCTTC 5 cut(s) 40, 489, 1058, 2280, 2682
Eco31I GGTCTC 3 cut(s) 2126, 2366, 2481
Eco57I CTGAAG 3 cut(s) 294, 1245, 1997
Eco81I CCTNAGG 1 cut(s) 1736
Eco88I CYCGRG 1 cut(s) 2640
EcoNI CCTNNNNNAGG 1 cut(s) 916
EcoO109I RGGNCCY 1 cut(s) 2381
EcoT22I ATGCAT 1 cut(s) 2812
FaeI CATG 6 cut(s) 684, 911, 1357, 1511, 1643, 2124
FalI AAGNNNNNCTT 6 cut(s) 411, 443, 896, 928, 2661, 2693
FaqI GGGAC 4 cut(s) 60, 1091, 2529, 2882
FatI CATG 6 cut(s) 680, 907, 1353, 1507, 1639, 2120
FauI CCCGC 1 cut(s) 2871
FauNDI CATATG 1 cut(s) 1926
FblI GTMKAC 2 cut(s) 84, 639
Fnu4HI GCNGC 8 cut(s) 22, 143, 243, 284, 313, 316, 632, 999
Fsp4HI GCNGC 8 cut(s) 22, 143, 243, 284, 313, 316, 632, 999
FspBI CTAG 6 cut(s) 651, 947, 1586, 1989, 2111, 2697
FspI TGCGCA 2 cut(s) 630, 1577
GlaI GCGC 4 cut(s) 25, 630, 1577, 1880
GluI GCNGC 8 cut(s) 22, 143, 243, 284, 313, 316, 632, 999
GsaI CCCAGC 1 cut(s) 200
GsuI CTGGAG 1 cut(s) 322
HaeII RGCGCY 1 cut(s) 27
HaeIII GGCC 4 cut(s) 68, 533, 2383, 2645
HapII CCGG 1 cut(s) 590
HhaI GCGC 4 cut(s) 26, 631, 1578, 1881
Hin1II CATG 6 cut(s) 684, 911, 1357, 1511, 1643, 2124
Hin6I GCGC 4 cut(s) 24, 629, 1576, 1879
HinP1I GCGC 4 cut(s) 24, 629, 1576, 1879
HincII GTYRAC 1 cut(s) 85
HindII GTYRAC 1 cut(s) 85
HindIII AAGCTT 1 cut(s) 1551
HpaII CCGG 1 cut(s) 590
HphI GGTGA 1 cut(s) 1230
Hpy166II GTNNAC 8 cut(s) 85, 98, 508, 640, 1031, 2027, 2294, 2627
Hpy188III TCNNGA 9 cut(s) 468, 947, 1392, 1611, 1640, 1886, 2234, 2684, 2909
Hpy8I GTNNAC 8 cut(s) 85, 98, 508, 640, 1031, 2027, 2294, 2627
Hpy99I CGWCG 3 cut(s) 83, 86, 221
HpyCH4III ACNGT 6 cut(s) 503, 512, 847, 1657, 1760, 2738
HpyCH4IV ACGT 5 cut(s) 48, 814, 823, 1266, 1811
HpyF10VI GCNNNNNNNGC 3 cut(s) 239, 289, 2078
HpyF3I CTNAG 8 cut(s) 928, 1736, 1842, 2130, 2201, 2385, 2568, 2901
HpySE526I ACGT 5 cut(s) 48, 814, 823, 1266, 1811
Hsp92II CATG 6 cut(s) 684, 911, 1357, 1511, 1643, 2124
HspAI GCGC 4 cut(s) 24, 629, 1576, 1879
KpnI GGTACC 1 cut(s) 1637
Kzo9I GATC 9 cut(s) 105, 264, 333, 458, 798, 1642, 1942, 2248, 2426
LmnI GCTCC 3 cut(s) 2126, 2309, 2504
Lsp1109I GCAGC 6 cut(s) 129, 229, 324, 327, 643, 985
MaeI CTAG 6 cut(s) 651, 947, 1586, 1989, 2111, 2697
MaeII ACGT 5 cut(s) 48, 814, 823, 1266, 1811
MalI GATC 9 cut(s) 107, 266, 335, 460, 800, 1644, 1944, 2250, 2428
MboI GATC 9 cut(s) 105, 264, 333, 458, 798, 1642, 1942, 2248, 2426
MfeI CAATTG 2 cut(s) 12, 1825
MflI RGATCY 3 cut(s) 333, 1942, 2426
MhlI GDGCHC 3 cut(s) 165, 1677, 2629
MlyI GAGTC 5 cut(s) 785, 1337, 1418, 1444, 1860
MmeI TCCRAC 4 cut(s) 965, 1249, 1392, 2759
Mph1103I ATGCAT 1 cut(s) 2812
MroXI GAANNNNTTC 1 cut(s) 185
MslI CAYNNNNRTG 4 cut(s) 753, 1320, 1887, 2805
MspA1I CMGCKG 4 cut(s) 114, 200, 932, 2866
MspCI CTTAAG 1 cut(s) 2207
MspI CCGG 1 cut(s) 590
MunI CAATTG 2 cut(s) 12, 1825
Mva1269I GAATGC 2 cut(s) 1315, 2810
MwoI GCNNNNNNNGC 3 cut(s) 239, 289, 2078
NdeI CATATG 1 cut(s) 1926
NdeII GATC 9 cut(s) 105, 264, 333, 458, 798, 1642, 1942, 2248, 2426
NlaIII CATG 6 cut(s) 684, 911, 1357, 1511, 1643, 2124
NlaIV GGNNCC 2 cut(s) 369, 1635
NmuCI GTSAC 8 cut(s) 805, 847, 1236, 1421, 1691, 1958, 2338, 2732
NsbI TGCGCA 2 cut(s) 630, 1577
NsiI ATGCAT 1 cut(s) 2812
NspI RCATGY 1 cut(s) 911
PaeI GCATGC 1 cut(s) 911
PagI TCATGA 1 cut(s) 1639
PcsI WCGNNNNNNNCGW 2 cut(s) 642, 820
PctI GAATGC 2 cut(s) 1315, 2810
PdmI GAANNNNTTC 1 cut(s) 185
PfeI GAWTC 5 cut(s) 305, 586, 2141, 2656, 2846
PkrI GCNGC 8 cut(s) 23, 144, 244, 285, 314, 317, 633, 1000
PleI GAGTC 5 cut(s) 784, 1336, 1418, 1444, 1860
PpsI GAGTC 5 cut(s) 784, 1336, 1418, 1444, 1860
Ppu21I YACGTR 1 cut(s) 815
Psp1406I AACGTT 1 cut(s) 1811
PspFI CCCAGC 1 cut(s) 196
PspN4I GGNNCC 2 cut(s) 369, 1635
PspPI GGNCC 2 cut(s) 2381, 2643
PstI CTGCAG 1 cut(s) 247
PsuI RGATCY 3 cut(s) 333, 1942, 2426
PvuII CAGCTG 2 cut(s) 200, 932
RsaI GTAC 9 cut(s) 330, 509, 844, 1280, 1635, 1792, 2026, 2224, 2704
RsaNI GTAC 9 cut(s) 329, 508, 843, 1279, 1634, 1791, 2025, 2223, 2703
RseI CAYNNNNRTG 4 cut(s) 753, 1320, 1887, 2805
SalI GTCGAC 1 cut(s) 83
SatI GCNGC 8 cut(s) 22, 143, 243, 284, 313, 316, 632, 999
Sau3AI GATC 9 cut(s) 105, 264, 333, 458, 798, 1642, 1942, 2248, 2426
Sau96I GGNCC 2 cut(s) 2381, 2643
ScaI AGTACT 1 cut(s) 1280
SchI GAGTC 5 cut(s) 785, 1337, 1418, 1444, 1860
SduI GDGCHC 3 cut(s) 165, 1677, 2629
SfcI CTRYAG 1 cut(s) 243
SmiMI CAYNNNNRTG 4 cut(s) 753, 1320, 1887, 2805
SmlI CTYRAG 5 cut(s) 389, 723, 2207, 2594, 2909
SmoI CTYRAG 5 cut(s) 389, 723, 2207, 2594, 2909
SphI GCATGC 1 cut(s) 911
SsiI CCGC 9 cut(s) 5, 21, 114, 173, 283, 763, 963, 2700, 2864
SspI AATATT 3 cut(s) 1059, 2053, 2917
SspMI CTAG 6 cut(s) 651, 947, 1586, 1989, 2111, 2697
TaaI ACNGT 6 cut(s) 503, 512, 847, 1657, 1760, 2738
TaiI ACGT 5 cut(s) 51, 817, 826, 1269, 1814
TaqI TCGA 8 cut(s) 84, 165, 216, 294, 1166, 1298, 1610, 2283
TaqII GACCGA 1 cut(s) 2150
TatI WGTACW 2 cut(s) 1278, 1790
TauI GCSGC 2 cut(s) 24, 286
TfiI GAWTC 5 cut(s) 305, 586, 2141, 2656, 2846
TscAI CASTG 3 cut(s) 755, 2322, 2807
TseFI GTSAC 8 cut(s) 805, 847, 1236, 1421, 1691, 1958, 2338, 2732
TseI GCWGC 6 cut(s) 142, 242, 312, 315, 631, 998
Tsp45I GTSAC 8 cut(s) 805, 847, 1236, 1421, 1691, 1958, 2338, 2732
TspDTI ATGAA 7 cut(s) 318, 363, 1068, 1496, 1848, 2036, 2379
TspGWI ACGGA 4 cut(s) 83, 346, 804, 2121
TspRI CASTG 3 cut(s) 755, 2322, 2807
Vha464I CTTAAG 1 cut(s) 2207
VneI GTGCAC 1 cut(s) 2625
XagI CCTNNNNNAGG 1 cut(s) 916
XapI RAATTY 7 cut(s) 350, 401, 701, 1187, 1397, 1544, 1614
XbaI TCTAGA 1 cut(s) 946
XceI RCATGY 1 cut(s) 911
XcmI CCANNNNNNNNNTGG 1 cut(s) 487
XmiI GTMKAC 2 cut(s) 84, 639
XmnI GAANNNNTTC 1 cut(s) 185
XspI CTAG 6 cut(s) 651, 947, 1586, 1989, 2111, 2697
ZrmI AGTACT 1 cut(s) 1280
Zsp2I ATGCAT 1 cut(s) 2812
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.