Belongs to the disease resistance NB-LRR family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 539 |
| Acc36I | ACCTGC | 1 cut(s) | 2935 |
| AccB1I | GGYRCC | 1 cut(s) | 766 |
| AccB7I | CCANNNNNTGG | 3 cut(s) | 2405, 2701, 2824 |
| AccII | CGCG | 1 cut(s) | 93 |
| AccIII | TCCGGA | 2 cut(s) | 308, 2320 |
| AciI | CCGC | 8 cut(s) | 91, 93, 318, 321, 1819, 2492, 3472, 3676 |
| AclI | AACGTT | 1 cut(s) | 2623 |
| AclWI | GGATC | 2 cut(s) | 1160, 1836 |
| AcoI | YGGCCR | 1 cut(s) | 3012 |
| AcuI | CTGAAG | 7 cut(s) | 26, 247, 291, 2588, 2852, 3281, 3660 |
| AcyI | GRCGYC | 1 cut(s) | 767 |
| AfaI | GTAC | 9 cut(s) | 656, 1462, 1653, 1772, 1873, 2186, 2311, 2561, 3302 |
| AhlI | ACTAGT | 2 cut(s) | 1193, 1948 |
| AjnI | CCWGG | 2 cut(s) | 2823, 2836 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1056, 1088 |
| Alw21I | GWGCWC | 3 cut(s) | 162, 2094, 3144 |
| Alw26I | GTCTC | 7 cut(s) | 746, 988, 1151, 1522, 1558, 2264, 2328 |
| AlwI | GGATC | 2 cut(s) | 1160, 1836 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 2701, 3615 |
| Aor13HI | TCCGGA | 2 cut(s) | 308, 2320 |
| AoxI | GGCC | 2 cut(s) | 2706, 3012 |
| ArsI | GACNNNNNNTTYG | 6 cut(s) | 745, 777, 1307, 1339, 1567, 1599 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 899 |
| AspLEI | GCGC | 1 cut(s) | 769 |
| AspS9I | GGNCC | 3 cut(s) | 247, 1342, 3726 |
| AsuHPI | GGTGA | 3 cut(s) | 2495, 2585, 3137 |
| AvaII | GGWCC | 3 cut(s) | 247, 1342, 3726 |
| AxyI | CCTNAGG | 1 cut(s) | 2913 |
| BaeGI | GKGCMC | 1 cut(s) | 974 |
| BalI | TGGCCA | 1 cut(s) | 3014 |
| BanI | GGYRCC | 1 cut(s) | 766 |
| BanII | GRGCYC | 4 cut(s) | 384, 2094, 2725, 3144 |
| BauI | CACGAG | 1 cut(s) | 650 |
| BbsI | GAAGAC | 3 cut(s) | 1526, 1951, 2858 |
| Bbv12I | GWGCWC | 3 cut(s) | 162, 2094, 3144 |
| BceAI | ACGGC | 1 cut(s) | 2488 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 938, 972 |
| BciT130I | CCWGG | 2 cut(s) | 2825, 2838 |
| BciVI | GTATCC | 1 cut(s) | 671 |
| BclI | TGATCA | 1 cut(s) | 963 |
| BcoDI | GTCTC | 7 cut(s) | 746, 988, 1151, 1522, 1558, 2264, 2328 |
| BcuI | ACTAGT | 2 cut(s) | 1193, 1948 |
| BfmI | CTRYAG | 2 cut(s) | 1359, 2027 |
| BfoI | RGCGCY | 1 cut(s) | 770 |
| BfuAI | ACCTGC | 1 cut(s) | 2935 |
| BfuI | GTATCC | 1 cut(s) | 671 |
| BglII | AGATCT | 1 cut(s) | 1363 |
| BlpI | GCTNAGC | 1 cut(s) | 645 |
| BmcAI | AGTACT | 1 cut(s) | 3302 |
| Bme1390I | CCNGG | 2 cut(s) | 2825, 2838 |
| Bme18I | GGWCC | 3 cut(s) | 247, 1342, 3726 |
| BmgT120I | GGNCC | 3 cut(s) | 247, 1342, 3726 |
| BmiI | GGNNCC | 3 cut(s) | 381, 768, 2724 |
| BmrFI | CCNGG | 2 cut(s) | 2825, 2838 |
| BmrI | ACTGGG | 1 cut(s) | 3223 |
| BmsI | GCATC | 7 cut(s) | 157, 229, 1822, 1986, 2057, 2804, 3681 |
| BmuI | ACTGGG | 1 cut(s) | 3223 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1974 |
| BpiI | GAAGAC | 3 cut(s) | 1526, 1951, 2858 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 12, 44 |
| BpmI | CTGGAG | 4 cut(s) | 1542, 2078, 2846, 3611 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 645 |
| BpuEI | CTTGAG | 2 cut(s) | 674, 3350 |
| BsaHI | GRCGYC | 1 cut(s) | 767 |
| BsaI | GGTCTC | 2 cut(s) | 1151, 2328 |
| BsaJI | CCNNGG | 5 cut(s) | 91, 171, 243, 1733, 2717 |
| BsaWI | WCCGGW | 2 cut(s) | 308, 2320 |
| Bse1I | ACTGG | 5 cut(s) | 1070, 1569, 2801, 3229, 3628 |
| Bse21I | CCTNAGG | 1 cut(s) | 2913 |
| Bse3DI | GCAATG | 1 cut(s) | 3422 |
| BseAI | TCCGGA | 2 cut(s) | 308, 2320 |
| BseBI | CCWGG | 2 cut(s) | 2825, 2838 |
| BseDI | CCNNGG | 5 cut(s) | 91, 171, 243, 1733, 2717 |
| BseMI | GCAATG | 1 cut(s) | 3422 |
| BseNI | ACTGG | 5 cut(s) | 1070, 1569, 2801, 3229, 3628 |
| BseRI | GAGGAG | 1 cut(s) | 518 |
| BseSI | GKGCMC | 1 cut(s) | 974 |
| BsgI | GTGCAG | 3 cut(s) | 1587, 2777, 3503 |
| Bsh1236I | CGCG | 1 cut(s) | 93 |
| BshFI | GGCC | 2 cut(s) | 2708, 3014 |
| BshNI | GGYRCC | 1 cut(s) | 766 |
| BsiHKAI | GWGCWC | 3 cut(s) | 162, 2094, 3144 |
| BsiSI | CCGG | 2 cut(s) | 309, 2321 |
| BsiWI | CGTACG | 1 cut(s) | 1651 |
| BslFI | GGGAC | 2 cut(s) | 896, 2613 |
| BsmAI | GTCTC | 7 cut(s) | 746, 988, 1151, 1522, 1558, 2264, 2328 |
| BsmFI | GGGAC | 2 cut(s) | 896, 2613 |
| BsmI | GAATGC | 1 cut(s) | 364 |
| BsnI | GGCC | 2 cut(s) | 2708, 3014 |
| Bso31I | GGTCTC | 2 cut(s) | 1151, 2328 |
| Bsp1286I | GDGCHC | 6 cut(s) | 162, 384, 974, 2094, 2725, 3144 |
| Bsp13I | TCCGGA | 2 cut(s) | 308, 2320 |
| Bsp1407I | TGTACA | 1 cut(s) | 654 |
| Bsp143I | GATC | 8 cut(s) | 784, 963, 1154, 1165, 1363, 1426, 1723, 1828 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 645 |
| BspACI | CCGC | 8 cut(s) | 91, 93, 318, 321, 1819, 2492, 3472, 3676 |
| BspANI | GGCC | 2 cut(s) | 2708, 3014 |
| BspEI | TCCGGA | 2 cut(s) | 308, 2320 |
| BspFNI | CGCG | 1 cut(s) | 93 |
| BspLI | GGNNCC | 3 cut(s) | 381, 768, 2724 |
| BspMAI | CTGCAG | 1 cut(s) | 1363 |
| BspMI | ACCTGC | 1 cut(s) | 2935 |
| BspPI | GGATC | 2 cut(s) | 1160, 1836 |
| BspT107I | GGYRCC | 1 cut(s) | 766 |
| BspTNI | GGTCTC | 2 cut(s) | 1151, 2328 |
| BsrDI | GCAATG | 1 cut(s) | 3422 |
| BsrGI | TGTACA | 1 cut(s) | 654 |
| BsrI | ACTGG | 5 cut(s) | 1070, 1569, 2801, 3229, 3628 |
| BssECI | CCNNGG | 5 cut(s) | 91, 171, 243, 1733, 2717 |
| BssMI | GATC | 8 cut(s) | 784, 963, 1154, 1165, 1363, 1426, 1723, 1828 |
| BssNI | GRCGYC | 1 cut(s) | 767 |
| BssSI | CACGAG | 1 cut(s) | 650 |
| BssT1I | CCWWGG | 2 cut(s) | 1733, 2717 |
| Bst2BI | CACGAG | 1 cut(s) | 650 |
| Bst2UI | CCWGG | 2 cut(s) | 2825, 2838 |
| Bst6I | CTCTTC | 4 cut(s) | 1385, 1407, 3300, 3636 |
| BstACI | GRCGYC | 1 cut(s) | 767 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 1037, 1127 |
| BstAUI | TGTACA | 1 cut(s) | 654 |
| BstC8I | GCNNGC | 8 cut(s) | 7, 486, 1038, 1119, 1239, 1748, 2696, 3474 |
| BstDSI | CCRYGG | 1 cut(s) | 91 |
| BstEII | GGTNACC | 3 cut(s) | 1714, 1854, 2646 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 3201 |
| BstFNI | CGCG | 1 cut(s) | 93 |
| BstH2I | RGCGCY | 1 cut(s) | 770 |
| BstHHI | GCGC | 1 cut(s) | 769 |
| BstKTI | GATC | 8 cut(s) | 787, 966, 1157, 1168, 1366, 1429, 1726, 1831 |
| BstMAI | GTCTC | 7 cut(s) | 746, 988, 1151, 1522, 1558, 2264, 2328 |
| BstMBI | GATC | 8 cut(s) | 784, 963, 1154, 1165, 1363, 1426, 1723, 1828 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 217, 1037, 1127, 1450, 1819, 2162, 2219, 3421 |
| BstNI | CCWGG | 2 cut(s) | 2825, 2838 |
| BstNSI | RCATGY | 1 cut(s) | 1040 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1974 |
| BstPI | GGTNACC | 3 cut(s) | 1714, 1854, 2646 |
| BstSCI | CCNGG | 2 cut(s) | 2823, 2836 |
| BstSFI | CTRYAG | 2 cut(s) | 1359, 2027 |
| BstSLI | GKGCMC | 1 cut(s) | 974 |
| BstUI | CGCG | 1 cut(s) | 93 |
| BstV2I | GAAGAC | 3 cut(s) | 1526, 1951, 2858 |
| BstX2I | RGATCY | 3 cut(s) | 1165, 1363, 1828 |
| BstYI | RGATCY | 3 cut(s) | 1165, 1363, 1828 |
| Bsu36I | CCTNAGG | 1 cut(s) | 2913 |
| BsuI | GTATCC | 1 cut(s) | 671 |
| BsuRI | GGCC | 2 cut(s) | 2708, 3014 |
| BtgI | CCRYGG | 1 cut(s) | 91 |
| BtsI | GCAGTG | 3 cut(s) | 1289, 1839, 3491 |
| BveI | ACCTGC | 1 cut(s) | 2935 |
| Cac8I | GCNNGC | 8 cut(s) | 7, 486, 1038, 1119, 1239, 1748, 2696, 3474 |
| CaiI | CAGNNNCTG | 2 cut(s) | 2701, 3615 |
| CfoI | GCGC | 1 cut(s) | 769 |
| Cfr13I | GGNCC | 3 cut(s) | 247, 1342, 3726 |
| Cfr42I | CCGCGG | 1 cut(s) | 94 |
| CseI | GACGC | 2 cut(s) | 1701, 1949 |
| CsiI | ACCWGGT | 1 cut(s) | 2836 |
| Csp6I | GTAC | 9 cut(s) | 655, 1461, 1652, 1771, 1872, 2185, 2310, 2560, 3301 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 2044, 2079, 2566, 2601 |
| CviQI | GTAC | 9 cut(s) | 655, 1461, 1652, 1771, 1872, 2185, 2310, 2560, 3301 |
| DinI | GGCGCC | 1 cut(s) | 768 |
| DpnI | GATC | 8 cut(s) | 786, 965, 1156, 1167, 1365, 1428, 1725, 1830 |
| DpnII | GATC | 8 cut(s) | 784, 963, 1154, 1165, 1363, 1426, 1723, 1828 |
| DraI | TTTAAA | 1 cut(s) | 2509 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 539 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 539 |
| EaeI | YGGCCR | 1 cut(s) | 3012 |
| Eam1104I | CTCTTC | 4 cut(s) | 1385, 1407, 3300, 3636 |
| EarI | CTCTTC | 4 cut(s) | 1385, 1407, 3300, 3636 |
| EciI | GGCGGA | 1 cut(s) | 336 |
| Ecl136II | GAGCTC | 2 cut(s) | 2092, 3142 |
| Eco130I | CCWWGG | 2 cut(s) | 1733, 2717 |
| Eco24I | GRGCYC | 4 cut(s) | 384, 2094, 2725, 3144 |
| Eco31I | GGTCTC | 2 cut(s) | 1151, 2328 |
| Eco47I | GGWCC | 3 cut(s) | 247, 1342, 3726 |
| Eco53kI | GAGCTC | 2 cut(s) | 2092, 3142 |
| Eco57I | CTGAAG | 7 cut(s) | 26, 247, 291, 2588, 2852, 3281, 3660 |
| Eco81I | CCTNAGG | 1 cut(s) | 2913 |
| Eco91I | GGTNACC | 3 cut(s) | 1714, 1854, 2646 |
| EcoICRI | GAGCTC | 2 cut(s) | 2092, 3142 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 3201 |
| EcoO109I | RGGNCCY | 1 cut(s) | 247 |
| EcoO65I | GGTNACC | 3 cut(s) | 1714, 1854, 2646 |
| EcoRI | GAATTC | 1 cut(s) | 324 |
| EcoRII | CCWGG | 2 cut(s) | 2823, 2836 |
| EcoT14I | CCWWGG | 2 cut(s) | 1733, 2717 |
| EcoT22I | ATGCAT | 2 cut(s) | 364, 1488 |
| EcoT38I | GRGCYC | 4 cut(s) | 384, 2094, 2725, 3144 |
| EgeI | GGCGCC | 1 cut(s) | 768 |
| EheI | GGCGCC | 1 cut(s) | 768 |
| ErhI | CCWWGG | 2 cut(s) | 1733, 2717 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 195, 227, 665, 697 |
| FaqI | GGGAC | 2 cut(s) | 896, 2613 |
| FauI | CCCGC | 1 cut(s) | 3465 |
| FauNDI | CATATG | 1 cut(s) | 3017 |
| FbaI | TGATCA | 1 cut(s) | 963 |
| FriOI | GRGCYC | 4 cut(s) | 384, 2094, 2725, 3144 |
| GlaI | GCGC | 1 cut(s) | 768 |
| GsuI | CTGGAG | 4 cut(s) | 1542, 2078, 2846, 3611 |
| HaeII | RGCGCY | 1 cut(s) | 770 |
| HaeIII | GGCC | 2 cut(s) | 2708, 3014 |
| HapII | CCGG | 2 cut(s) | 309, 2321 |
| HgaI | GACGC | 2 cut(s) | 1701, 1949 |
| HhaI | GCGC | 1 cut(s) | 769 |
| Hin1I | GRCGYC | 1 cut(s) | 767 |
| Hin6I | GCGC | 1 cut(s) | 767 |
| HinP1I | GCGC | 1 cut(s) | 767 |
| HincII | GTYRAC | 3 cut(s) | 82, 1954, 2983 |
| HindII | GTYRAC | 3 cut(s) | 82, 1954, 2983 |
| HindIII | AAGCTT | 3 cut(s) | 273, 2429, 3007 |
| HpaII | CCGG | 2 cut(s) | 309, 2321 |
| HphI | GGTGA | 3 cut(s) | 2495, 2585, 3137 |
| Hpy166II | GTNNAC | 7 cut(s) | 82, 655, 1954, 2983, 3128, 3371, 3542 |
| Hpy8I | GTNNAC | 7 cut(s) | 82, 655, 1954, 2983, 3128, 3371, 3542 |
| HpyCH4IV | ACGT | 2 cut(s) | 576, 2623 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 217, 1037, 1127, 1450, 1819, 2162, 2219, 3421 |
| HpySE526I | ACGT | 2 cut(s) | 576, 2623 |
| Hsp92I | GRCGYC | 1 cut(s) | 767 |
| HspAI | GCGC | 1 cut(s) | 767 |
| KasI | GGCGCC | 1 cut(s) | 766 |
| Kpn2I | TCCGGA | 2 cut(s) | 308, 2320 |
| Ksp22I | TGATCA | 1 cut(s) | 963 |
| KspI | CCGCGG | 1 cut(s) | 94 |
| Kzo9I | GATC | 8 cut(s) | 784, 963, 1154, 1165, 1363, 1426, 1723, 1828 |
| LmnI | GCTCC | 7 cut(s) | 20, 379, 2097, 2608, 2728, 3090, 3630 |
| LweI | GCATC | 7 cut(s) | 157, 229, 1822, 1986, 2057, 2804, 3681 |
| MabI | ACCWGGT | 1 cut(s) | 2836 |
| MaeII | ACGT | 2 cut(s) | 576, 2623 |
| MaeIII | GTNAC | 9 cut(s) | 934, 1714, 1854, 2137, 2412, 2573, 2646, 3238, 3400 |
| MalI | GATC | 8 cut(s) | 786, 965, 1156, 1167, 1365, 1428, 1725, 1830 |
| MboI | GATC | 8 cut(s) | 784, 963, 1154, 1165, 1363, 1426, 1723, 1828 |
| MfeI | CAATTG | 2 cut(s) | 998, 1883 |
| MflI | RGATCY | 3 cut(s) | 1165, 1363, 1828 |
| MhlI | GDGCHC | 6 cut(s) | 162, 384, 974, 2094, 2725, 3144 |
| MlsI | TGGCCA | 1 cut(s) | 3014 |
| MluNI | TGGCCA | 1 cut(s) | 3014 |
| Mly113I | GGCGCC | 1 cut(s) | 767 |
| MlyI | GAGTC | 5 cut(s) | 515, 1064, 1570, 2837, 3050 |
| MmeI | TCCRAC | 3 cut(s) | 357, 1521, 2427 |
| Mox20I | TGGCCA | 1 cut(s) | 3014 |
| Mph1103I | ATGCAT | 2 cut(s) | 364, 1488 |
| MroI | TCCGGA | 2 cut(s) | 308, 2320 |
| MroXI | GAANNNNTTC | 1 cut(s) | 899 |
| MscI | TGGCCA | 1 cut(s) | 3014 |
| MslI | CAYNNNNRTG | 4 cut(s) | 700, 1903, 2223, 2991 |
| Msp20I | TGGCCA | 1 cut(s) | 3014 |
| MspA1I | CMGCKG | 3 cut(s) | 93, 318, 2701 |
| MspI | CCGG | 2 cut(s) | 309, 2321 |
| MspR9I | CCNGG | 2 cut(s) | 2825, 2838 |
| MunI | CAATTG | 2 cut(s) | 998, 1883 |
| Mva1269I | GAATGC | 1 cut(s) | 364 |
| MvaI | CCWGG | 2 cut(s) | 2825, 2838 |
| MvnI | CGCG | 1 cut(s) | 93 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 217, 1037, 1127, 1450, 1819, 2162, 2219, 3421 |
| NarI | GGCGCC | 1 cut(s) | 767 |
| NdeI | CATATG | 1 cut(s) | 3017 |
| NdeII | GATC | 8 cut(s) | 784, 963, 1154, 1165, 1363, 1426, 1723, 1828 |
| NlaIV | GGNNCC | 3 cut(s) | 381, 768, 2724 |
| NmeAIII | GCCGAG | 2 cut(s) | 196, 268 |
| NmuCI | GTSAC | 3 cut(s) | 934, 2573, 3400 |
| NsiI | ATGCAT | 2 cut(s) | 364, 1488 |
| NspI | RCATGY | 1 cut(s) | 1040 |
| PaeI | GCATGC | 1 cut(s) | 1040 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1747 |
| PctI | GAATGC | 1 cut(s) | 364 |
| PdmI | GAANNNNTTC | 1 cut(s) | 899 |
| PfeI | GAWTC | 8 cut(s) | 299, 305, 601, 913, 1208, 1793, 2228, 3032 |
| Pfl23II | CGTACG | 1 cut(s) | 1651 |
| PflFI | GACNNNGTC | 1 cut(s) | 1900 |
| PflMI | CCANNNNNTGG | 3 cut(s) | 2405, 2701, 2824 |
| PfoI | TCCNGGA | 1 cut(s) | 2823 |
| PleI | GAGTC | 5 cut(s) | 514, 1063, 1570, 2836, 3049 |
| PluTI | GGCGCC | 1 cut(s) | 770 |
| PpsI | GAGTC | 5 cut(s) | 514, 1063, 1570, 2836, 3049 |
| PpuMI | RGGWCCY | 1 cut(s) | 247 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1974 |
| Psp124BI | GAGCTC | 2 cut(s) | 2094, 3144 |
| Psp1406I | AACGTT | 1 cut(s) | 2623 |
| Psp5II | RGGWCCY | 1 cut(s) | 247 |
| Psp6I | CCWGG | 2 cut(s) | 2823, 2836 |
| PspEI | GGTNACC | 3 cut(s) | 1714, 1854, 2646 |
| PspGI | CCWGG | 2 cut(s) | 2823, 2836 |
| PspLI | CGTACG | 1 cut(s) | 1651 |
| PspN4I | GGNNCC | 3 cut(s) | 381, 768, 2724 |
| PspPI | GGNCC | 3 cut(s) | 247, 1342, 3726 |
| PspPPI | RGGWCCY | 1 cut(s) | 247 |
| PstI | CTGCAG | 1 cut(s) | 1363 |
| PstNI | CAGNNNCTG | 2 cut(s) | 2701, 3615 |
| PsuI | RGATCY | 3 cut(s) | 1165, 1363, 1828 |
| PsyI | GACNNNGTC | 1 cut(s) | 1900 |
| PvuII | CAGCTG | 1 cut(s) | 2701 |
| RsaI | GTAC | 9 cut(s) | 656, 1462, 1653, 1772, 1873, 2186, 2311, 2561, 3302 |
| RsaNI | GTAC | 9 cut(s) | 655, 1461, 1652, 1771, 1872, 2185, 2310, 2560, 3301 |
| RseI | CAYNNNNRTG | 4 cut(s) | 700, 1903, 2223, 2991 |
| SacI | GAGCTC | 2 cut(s) | 2094, 3144 |
| SacII | CCGCGG | 1 cut(s) | 94 |
| Sau3AI | GATC | 8 cut(s) | 784, 963, 1154, 1165, 1363, 1426, 1723, 1828 |
| Sau96I | GGNCC | 3 cut(s) | 247, 1342, 3726 |
| ScaI | AGTACT | 1 cut(s) | 3302 |
| SchI | GAGTC | 5 cut(s) | 515, 1064, 1570, 2837, 3050 |
| ScrFI | CCNGG | 2 cut(s) | 2825, 2838 |
| SduI | GDGCHC | 6 cut(s) | 162, 384, 974, 2094, 2725, 3144 |
| SexAI | ACCWGGT | 1 cut(s) | 2836 |
| SfaNI | GCATC | 7 cut(s) | 157, 229, 1822, 1986, 2057, 2804, 3681 |
| SfcI | CTRYAG | 2 cut(s) | 1359, 2027 |
| SfoI | GGCGCC | 1 cut(s) | 768 |
| Sfr303I | CCGCGG | 1 cut(s) | 94 |
| SgrBI | CCGCGG | 1 cut(s) | 94 |
| SinI | GGWCC | 3 cut(s) | 247, 1342, 3726 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 700, 1903, 2223, 2991 |
| SmlI | CTYRAG | 2 cut(s) | 689, 3329 |
| SmoI | CTYRAG | 2 cut(s) | 689, 3329 |
| SpeI | ACTAGT | 2 cut(s) | 1193, 1948 |
| SphI | GCATGC | 1 cut(s) | 1040 |
| SsiI | CCGC | 8 cut(s) | 91, 93, 318, 321, 1819, 2492, 3472, 3676 |
| SspDI | GGCGCC | 1 cut(s) | 766 |
| SspI | AATATT | 2 cut(s) | 1305, 2300 |
| SstI | GAGCTC | 2 cut(s) | 2094, 3144 |
| StyD4I | CCNGG | 2 cut(s) | 2823, 2836 |
| StyI | CCWWGG | 2 cut(s) | 1733, 2717 |
| TaiI | ACGT | 2 cut(s) | 579, 2626 |
| TaqI | TCGA | 2 cut(s) | 54, 750 |
| TatI | WGTACW | 4 cut(s) | 654, 1460, 1770, 3300 |
| TauI | GCSGC | 3 cut(s) | 96, 321, 1822 |
| TfiI | GAWTC | 8 cut(s) | 299, 305, 601, 913, 1208, 1793, 2228, 3032 |
| TseFI | GTSAC | 3 cut(s) | 934, 2573, 3400 |
| Tsp45I | GTSAC | 3 cut(s) | 934, 2573, 3400 |
| TspGWI | ACGGA | 2 cut(s) | 145, 2723 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1900 |
| Van91I | CCANNNNNTGG | 3 cut(s) | 2405, 2701, 2824 |
| VpaK11BI | GGWCC | 3 cut(s) | 247, 1342, 3726 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 3201 |
| XbaI | TCTAGA | 2 cut(s) | 847, 1595 |
| XceI | RCATGY | 1 cut(s) | 1040 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 613, 2102 |
| XmnI | GAANNNNTTC | 1 cut(s) | 899 |
| ZrmI | AGTACT | 1 cut(s) | 3302 |
| Zsp2I | ATGCAT | 2 cut(s) | 364, 1488 |