Rorug05G0443200

F-box kelch-repeat protein At3g23880-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000068
Physical Location & Seq
Reverse (-)
61487295 .. 61491036
3742 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0443200.1

Sequence Viewer

Length: 2502 bp
ATGATGGGTCATAGAAAATTTGATCTGTGCATCTGGGTTTTGTCAATCTTGAGTTTGGGTTGCTGCTGTAGTGGGTTTACTCCTGTAGACAATTACTTGATCGATTGTGGTTCACTGGCCAATACTTCAGTGGGTAACCGTGTTTATCTAGCTGATAAGTTGGCTTCAAATTTCCTTTCAACCCCAAAAGATGTTCTTGCCAATAGTCCATTGAAATCAATCACTTCCTTTGATGATTCTCCGCTGTATCAAACAGCTAGAATCTTTACTGGGTCTTCAAAATACACTTTTTCAATAAGCCTGACTGGTAGGCACTGGATCCGCCTCTATTTCTATCCATTTGTTTCGGGTAGTTATGATCTGAGCAAGGCCAAATTCTCTGTTTCCACCCAAAACTATGGCCTGCTTAGTGATTTCAGCGTTCAGAACTCTTCTGTGATGAAAGAGTTTTCGGTGAATGTGACTTCGGGTAGCCTTGTTGTCACTTTCACCCCTTCTAGTGGTTCATTTGCATTCTTGAATGCCATTGAAGTTGTTTCAGTCCCCGACGCACTCATTACCGATGATGCCACCACTACCACTCCTGGTAAATTCCAGGGTTTGATGAGTCAGGCTCTGGAGACAACTTGGAGGGTGAATATGGGTGGACCATTGGTGTCTTTCGAGAATGATACCCTGTGGCGAACTTGGGTTCCTGATCAAGATTATCTAGTGAGCAAGAACTTGGCCAAAGATTTCTCAAATGTTGCAGCTGTTGAATATGCCGATGGTTTGGCAACTGCAGATAGTGCTCCCAGAACGGTTTACGGTACTCTCACAGAGATGAACTCAGCTGGTGATCCCAACACCAATTACAATGTGACCTGGGAGTTCAAAGTGGATTCAGGATTTCAATACCTTGTTCGCTTTCACTTTTGCGACATAGTAAGTAAAGGTCTAAATCAGCTTTACTTCAATGTGTATTTTGACTCTTGGATTGTTGCTCAAGATCTTGATCTGAGTACTATAGCAGTTAACAAGTTAGCTACACCATATTACTCGGATTATGTTACAACATCAGTTGTCAGCGATACAATTCGTATTAGTATTGGTCCTTCCTCTGTGAATAATGCTTATCCCAATGCCATTCTAAATGGGCTTGAGATTATGAAAATGAACAACTCAGATGGCCACCTTGGTGGAGCAAGCGGTGCAGTTAATTCAAATTCAAGTTCAAAAAGTAATGTCGGAGTTATAGTGGGTGCCACTGTTGGATCGCTTATTGCAGTGGTGTTGGCTGCTATTCTCTTTCTTCTGTGCAGAAGAAGGAAGGATAAGGCAAATCAGGGTCATTCTAAGACATGGGCTCCTTTCTCCATGAATGGAACCAATTCTCACACCATGGGAAGCAAGTACTCATACGGAACAACTGCTAGTGCTGCTTCAAACTACCAGTATCGCTTTCCTTTTGGGGTAGTTCAAGAGGCTACAAACAACTTTGACGAGAGTTGGGTTATCGGAATTGGTGGTTTTGGGAAGGTTTACAAAGGAGAATTTAATGATGGAACTAAAGTAGCTGTCAAGAGGGGAAACCCACGGTCTCAACAGGGGCTTGCAGAGTTCAGGACCGAAATTGAGATGCTTTCTCAATTCCGTCATCGCCATCTTGTGTCATTGATTGGGTATTGTGATGACAAGAGTGAGATGATTCTGATATACGAATATATGGAGAATGGGACCTTGAAGAGTCATCTCTATGGCTCTGGTCTACCCAGCTTAAGCTGGAAGGCAAGGCTTGAAATATGCATTGGATCAGCTAGAGGACTTCACTACCTTCACACTGGTGATGCAAAAGCAGTTATTCATCGCGATGTGAAGTCTGCGAATATCTTGCTTGATGAGAACCTCATGGCCAAGGTTGCTGATTTTGGGCTTTCCAAGACTGGTCCAGAACTTGATCAGACCCATGTGAGTACTGCAGTGAAAGGGAGTTTCGGATACCTTGACCCTGAATATTTTAGAAGGCAACAACTGACAGAGAAATCAGATGTGTATTCATTCGGGGTGGTTTTGTTTGAGGTTCTTTGTGCAAGACCTGTCATAGATCCATCCCTGCCCAGGGAAATGGTGAACTTGGCTGAGTGGGCGATGAAATGGCAGAAGAAAGGGCAGTTGGAGCAAATCATAGATCCTACACTTGCGGGAAAAATACGACCAGATTCTCTCAGAAAATTTGCAGAAACCGCGGAGAAATGCTTGGCTGACTATGGTGTTGACAGGCCCTCAATGGGAGATGTCTTGTGGAATCTGGAGTATGCCCTTCAACTTCAAGAGGCAGTAATTCAAGATCCTGAAGACAACAGTACCAATATGATCGGCGAGCTATCTCCACAAGTCAACAATTTCAATCACATTGACACAACTGGTTCTCCTGGACAGTTTGAAGTCTCAAGTGTGGATGATCTTTCAGGTGTTTCCATGAGTAGAGTATTCTCACAGCTGGTCAAGTCTGAGGGTAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

833

Amino Acids

91.63

Weight (kDa)

5.63

Isoelectric Point (pI)

27.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 34 - 384 1.2e-26 Malectin-like domain
Malectin PF11721 210 - 364 3.6e-06 Malectin domain
PK_Tyr_Ser-Thr PF07714 498 - 692 8.8e-47 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 498 - 756 2.6e-42 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000068)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g08281 FvH4_1g22270 FvH4_1g22290 FvH4_1g26450 FvH4_3g16521 FvH4_3g16840 FvH4_3g16850 FvH4_3g16850 FvH4_3g16860 FvH4_3g16860 FvH4_3g18860 FvH4_3g18860 FvH4_3g18860 FvH4_3g18860 FvH4_3g18860 FvH4_3g18860 FvH4_3g18860 FvH4_3g18860 FvH4_3g18860 FvH4_3g18860 FvH4_3g18870 FvH4_3g18890 FvH4_3g18890 FvH4_3g18890 FvH4_3g18910 FvH4_3g18910 FvH4_3g18910 FvH4_3g18910 FvH4_3g18911 FvH4_3g18920 FvH4_3g18920 FvH4_3g18920 FvH4_3g18950 FvH4_3g18950 FvH4_3g18960 FvH4_3g18960 FvH4_3g18960 FvH4_3g18960 FvH4_3g18960 FvH4_3g18960 FvH4_3g18970 FvH4_3g18971 FvH4_3g18972 FvH4_3g22371 FvH4_3g33911 FvH4_3g39320 FvH4_3g39330 FvH4_3g41891 FvH4_3g41900 FvH4_3g41900 FvH4_3g41900 FvH4_3g41900 FvH4_3g42380 FvH4_3g42380 FvH4_3g42380 FvH4_3g42390 FvH4_5g11821 FvH4_5g11823 FvH4_5g11830 FvH4_5g15943 FvH4_5g15980 FvH4_5g29450 FvH4_5g35130 FvH4_6g02391 FvH4_6g25310 FvH4_6g32080 FvH4_6g32080 FvH4_6g32080 FvH4_6g32080 FvH4_7g06030 FvH4_7g06030 FvH4_7g11650 FvH4_7g11650 FvH4_7g11650 FvH4_7g11650 FvH4_7g11650 FvH4_7g11650 FvH4_7g11670 FvH4_7g11690 FvH4_7g11770
malus_domestica MD03G1027200.v1.1 MD03G1027800.v1.1 MD03G1027900.v1.1 MD03G1028000.v1.1 MD03G1028100.v1.1 MD03G1028200.v1.1 MD03G1028300.v1.1 MD03G1028400.v1.1 MD05G1165700.v1.1 MD05G1206600.v1.1 MD09G1091100.v1.1 MD11G1031100.v1.1 MD11G1031400.v1.1 MD11G1031700.v1.1 MD12G1183200.v1.1 MD13G1129500.v1.1 MD15G1381400.v1.1 MD16G1129700.v1.1
prunus_persica Prupe.1G172200_v2.0.a1 Prupe.1G172300_v2.0.a1 Prupe.2G255600_v2.0.a1 Prupe.4G150700_v2.0.a1 Prupe.4G150900_v2.0.a1 Prupe.4G151000_v2.0.a1 Prupe.4G151100_v2.0.a1 Prupe.4G156000_v2.0.a1 Prupe.4G190200_v2.0.a1 Prupe.4G210300_v2.0.a1 Prupe.4G210300_v2.0.a1 Prupe.4G210300_v2.0.a1 Prupe.4G210300_v2.0.a1 Prupe.4G210300_v2.0.a1 Prupe.4G210300_v2.0.a1 Prupe.4G221400_v2.0.a1 Prupe.4G221500_v2.0.a1 Prupe.5G081800_v2.0.a1 Prupe.5G081900_v2.0.a1 Prupe.6G096300_v2.0.a1 Prupe.6G096300_v2.0.a1 Prupe.6G287700_v2.0.a1 Prupe.6G294100_v2.0.a1 Prupe.8G003300_v2.0.a1 Prupe.8G042400_v2.0.a1 Prupe.8G042400_v2.0.a1 Prupe.8G042400_v2.0.a1 Prupe.8G042400_v2.0.a1 Prupe.8G042400_v2.0.a1 Prupe.8G211600_v2.0.a1 Prupe.8G211700_v2.0.a1 Prupe.8G212800_v2.0.a1 Prupe.8G212900_v2.0.a1
pyrus_communis pycom03g02220 pycom03g02240 pycom03g02250 pycom03g02280 pycom03g02290 pycom03g02300 pycom04g15010 pycom05g19280 pycom08g08850 pycom09g01650 pycom09g01680 pycom11g02670 pycom11g23890 pycom13g11270 pycom13g25200 pycom16g10990
rosa_chinensis RchiOBHm_Chr1g0348511 RchiOBHm_Chr1g0350191 RchiOBHm_Chr2g0116421 RchiOBHm_Chr2g0116451 RchiOBHm_Chr2g0159381 RchiOBHm_Chr3g0482231 RchiOBHm_Chr3g0482251 RchiOBHm_Chr5g0003271 RchiOBHm_Chr5g0028051 RchiOBHm_Chr5g0030021 RchiOBHm_Chr5g0030031 RchiOBHm_Chr5g0031731 RchiOBHm_Chr5g0031741 RchiOBHm_Chr5g0031751 RchiOBHm_Chr5g0031761 RchiOBHm_Chr5g0031771 RchiOBHm_Chr5g0031801 RchiOBHm_Chr5g0039041 RchiOBHm_Chr5g0057531 RchiOBHm_Chr5g0061121 RchiOBHm_Chr5g0067481 RchiOBHm_Chr5g0067551 RchiOBHm_Chr5g0070761 RchiOBHm_Chr5g0075091 RchiOBHm_Chr5g0075101 RchiOBHm_Chr5g0075881 RchiOBHm_Chr6g0284591 RchiOBHm_Chr6g0284651
rosa_laevigata RLG00000004885 RLG00000012734 RLG00000018267 RLG00000018268 RLG00000018269 RLG00000018272 RLG00000021158 RLG00000021159 RLG00000023360 RLG00000025795 RLG00000028499 RLG00000033066 RLG00000033210 RLG00000033336 RLG00000033339 RLG00000033886 RLG00000034355 RLG00000036157 RLG00000036529
rosa_multiflora Rmu_co8023544.1_g000001 Rmu_co8070216.1_g000001 Rmu_co8115708.1_g000001 Rmu_co8182078.1_g000001 Rmu_co8269411.1_g000001 Rmu_sc0000533.1_g000049 Rmu_sc0001366.1_g000029 Rmu_sc0001366.1_g000030 Rmu_sc0001647.1_g000003 Rmu_sc0002121.1_g000005 Rmu_sc0002253.1_g000042 Rmu_sc0002652.1_g000020 Rmu_sc0002703.1_g000001 Rmu_sc0002944.1_g000029 Rmu_sc0002944.1_g000030 Rmu_sc0003368.1_g000004 Rmu_sc0003368.1_g000011 Rmu_sc0003819.1_g000014 Rmu_sc0004191.1_g000062 Rmu_sc0004191.1_g000064 Rmu_sc0004221.1_g000007 Rmu_sc0004800.1_g000015 Rmu_sc0005047.1_g000003 Rmu_sc0005047.1_g000004 Rmu_sc0005719.1_g000011 Rmu_sc0006847.1_g000006 Rmu_sc0006847.1_g000007 Rmu_sc0006847.1_g000009 Rmu_sc0006847.1_g000012 Rmu_sc0006847.1_g000014 Rmu_sc0006847.1_g000021 Rmu_sc0007753.1_g000009 Rmu_sc0009432.1_g000001 Rmu_sc0009958.1_g000006 Rmu_sc0010426.1_g000008 Rmu_sc0010618.1_g000001 Rmu_sc0010618.1_g000002 Rmu_sc0011187.1_g000003 Rmu_sc0015338.1_g000001 Rmu_sc0015338.1_g000008 Rmu_sc0015338.1_g000010 Rmu_sc0016526.1_g000004 Rmu_sc0017252.1_g000002 Rmu_sc0030815.1_g000002 Rmu_sc0040072.1_g000001 Rmu_sc0042974.1_g000001 Rmu_sc0042975.1_g000001 Rmu_ssc0000217.1_g000006 Rmu_ssc0000357.1_g000027
rosa_roxburghii Rroxscaffold_1G00005800 Rroxscaffold_1G00006520 Rroxscaffold_1G00010390 Rroxscaffold_1G00010420 Rroxscaffold_1G00013590 Rroxscaffold_1G00019550 Rroxscaffold_1G00042090 Rroxscaffold_1G00048480 Rroxscaffold_1G00049110 Rroxscaffold_1G00049120 Rroxscaffold_1G00049990 Rroxscaffold_1G00050980 Rroxscaffold_1G00051570 Rroxscaffold_1G00051610 Rroxscaffold_2G00090440 Rroxscaffold_2G00101710 Rroxscaffold_2G00127180 Rroxscaffold_2G00127200 Rroxscaffold_2G00147200 Rroxscaffold_3G00236860 Rroxscaffold_4G00305010 Rroxscaffold_4G00305020 Rroxscaffold_6G00399460 Rroxscaffold_6G00399470 Rroxscaffold_6G00399940 Rroxscaffold_6G00399950 Rroxscaffold_7G00210650
rosa_rugosa Rorug01G0209300 Rorug01G0209400 Rorug01G0209500 Rorug01G0209600 Rorug01G0209700 Rorug01G0209800 Rorug02G0043500 Rorug02G0206500 Rorug02G0206700 Rorug03G0195800 Rorug03G0195800 Rorug04G0105900 Rorug05G0103800 Rorug05G0111500 Rorug05G0176100 Rorug05G0178200 Rorug05G0337600 Rorug05G0337700 Rorug05G0385700 Rorug05G0436300 Rorug05G0438300 Rorug05G0438400 Rorug05G0438500 Rorug05G0438600 Rorug05G0438700 Rorug05G0443100.1 Rorug05G0443200 Rorug06G0164600
rosa_samantha Rh2CG525500 Rh2CG526300 Rh5AG399000 Rh5CG410800 Rh5CG437000 Rh5CG538800 Rh5DG197300 Rh5DG197600 Rh5DG226300 Rh5DG226400 Rh5DG226500 Rh5DG494400 Rh5DG494500 Rh5DG527500 Rh5DG534000 Rh6BG079300 Rh6CG186400
rosa_wichuraiana Rw0G017740 Rw1G019240 Rw1G019340 Rw2G020600 Rw3G022120 Rw3G022140 Rw5G018000 Rw5G018020 Rw5G018460 Rw5G020200 Rw5G020210 Rw5G020230 Rw5G024530 Rw5G037640 Rw5G039720 Rw5G043170 Rw5G045780 Rw5G046420 Rw6G023850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1241
AccI GTMKAC 2 cut(s) 87, 1747
AccII CGCG 2 cut(s) 1848, 2225
AciI CCGC 6 cut(s) 242, 322, 1188, 2180, 2223, 2225
AclWI GGATC 8 cut(s) 313, 326, 833, 1261, 1798, 2078, 2162, 2321
AcoI YGGCCR 4 cut(s) 117, 726, 1168, 1890
AcsI RAATTY 7 cut(s) 17, 169, 374, 590, 1204, 1532, 2210
AcuI CTGAAG 2 cut(s) 111, 2352
AfaI GTAC 5 cut(s) 811, 1003, 1394, 1954, 2344
AfiI CCNNNNNNNGG 4 cut(s) 500, 2097, 2098, 2267
AflII CTTAAG 1 cut(s) 1756
AjnI CCWGG 5 cut(s) 583, 594, 863, 2096, 2410
AjuI GAANNNNNNNTTGG 4 cut(s) 1770, 1802, 2135, 2167
AleI CACNNNNGTG 2 cut(s) 1176, 1821
AloI GAACNNNNNNTCC 2 cut(s) 676, 708
Alw21I GWGCWC 1 cut(s) 793
Alw26I GTCTC 3 cut(s) 614, 1584, 2431
AlwI GGATC 8 cut(s) 313, 326, 833, 1261, 1798, 2078, 2162, 2321
AlwNI CAGNNNCTG 1 cut(s) 616
AoxI GGCC 7 cut(s) 117, 369, 400, 726, 1168, 1890, 2258
ApeKI GCWGC 4 cut(s) 63, 749, 1277, 1418
ApoI RAATTY 7 cut(s) 17, 169, 374, 590, 1204, 1532, 2210
Asp700I GAANNNNTTC 1 cut(s) 1369
AspS9I GGNCC 6 cut(s) 647, 1091, 1605, 1716, 1925, 2259
AsuHPI GGTGA 6 cut(s) 466, 481, 646, 848, 1835, 2119
AvaII GGWCC 5 cut(s) 647, 1091, 1605, 1716, 1925
BaeI ACNNNNGTAYC 2 cut(s) 2326, 2359
BalI TGGCCA 4 cut(s) 119, 728, 1170, 1892
BamHI GGATCC 1 cut(s) 318
BanI GGYRCC 1 cut(s) 1241
BanII GRGCYC 1 cut(s) 1348
BarI GAAGNNNNNNTAC 2 cut(s) 1078, 1110
BbsI GAAGAC 2 cut(s) 267, 2340
Bbv12I GWGCWC 1 cut(s) 793
BbvI GCAGC 4 cut(s) 50, 761, 1264, 1405
BccI CCATC 5 cut(s) 761, 1160, 1535, 1650, 2095
BcgI CGANNNNNNTGC 2 cut(s) 1852, 1886
BciT130I CCWGG 5 cut(s) 585, 596, 865, 2098, 2412
BciVI GTATCC 1 cut(s) 1970
BclI TGATCA 2 cut(s) 697, 1936
BcoDI GTCTC 3 cut(s) 614, 1584, 2431
BfaI CTAG 6 cut(s) 149, 258, 498, 710, 1413, 1797
BfmI CTRYAG 5 cut(s) 67, 84, 780, 1005, 1956
BfrI CTTAAG 1 cut(s) 1756
BfuI GTATCC 1 cut(s) 1970
BglII AGATCT 1 cut(s) 988
BisI GCNGC 4 cut(s) 64, 750, 1278, 1419
BlsI GCNGC 4 cut(s) 65, 751, 1279, 1420
BmcAI AGTACT 3 cut(s) 1003, 1394, 1954
Bme1390I CCNGG 5 cut(s) 585, 596, 865, 2098, 2412
Bme18I GGWCC 5 cut(s) 647, 1091, 1605, 1716, 1925
BmgT120I GGNCC 6 cut(s) 647, 1091, 1605, 1716, 1925, 2259
BmiI GGNNCC 6 cut(s) 320, 693, 1243, 1347, 1366, 1717
BmrFI CCNGG 5 cut(s) 585, 596, 865, 2098, 2412
BmrI ACTGGG 1 cut(s) 279
BmsI GCATC 4 cut(s) 39, 556, 1608, 1816
BmuI ACTGGG 1 cut(s) 279
BpiI GAAGAC 2 cut(s) 267, 2340
BplI GAGNNNNNCTC 4 cut(s) 598, 630, 812, 844
BpmI CTGGAG 2 cut(s) 638, 2309
BpuEI CTTGAG 4 cut(s) 70, 969, 1160, 2413
Bsa29I ATCGAT 1 cut(s) 102
BsaBI GATNNNNATC 1 cut(s) 993
BsaI GGTCTC 1 cut(s) 1584
BsaJI CCNNGG 9 cut(s) 595, 864, 1174, 1380, 1574, 1893, 2096, 2097, 2223
BsaXI ACNNNNNCTCC 8 cut(s) 565, 595, 1221, 1251, 1330, 1360, 2392, 2422
Bsc4I CCNNNNNNNGG 4 cut(s) 500, 2097, 2098, 2267
Bse1I ACTGG 8 cut(s) 120, 274, 310, 320, 1432, 1825, 1927, 2407
Bse8I GATNNNNATC 1 cut(s) 993
BseBI CCWGG 5 cut(s) 585, 596, 865, 2098, 2412
BseCI ATCGAT 1 cut(s) 102
BseDI CCNNGG 9 cut(s) 595, 864, 1174, 1380, 1574, 1893, 2096, 2097, 2223
BseGI GGATG 2 cut(s) 2087, 2443
BseJI GATNNNNATC 1 cut(s) 993
BseLI CCNNNNNNNGG 4 cut(s) 500, 2097, 2098, 2267
BseMII CTCAG 7 cut(s) 353, 843, 989, 1176, 2109, 2218, 2481
BseNI ACTGG 8 cut(s) 120, 274, 310, 320, 1432, 1825, 1927, 2407
BseXI GCAGC 4 cut(s) 50, 761, 1264, 1405
BseYI CCCAGC 1 cut(s) 1751
BsgI GTGCAG 2 cut(s) 1212, 1318
Bsh1236I CGCG 2 cut(s) 1848, 2225
BshFI GGCC 7 cut(s) 119, 371, 402, 728, 1170, 1892, 2260
BshNI GGYRCC 1 cut(s) 1241
BshVI ATCGAT 1 cut(s) 102
BsiHKAI GWGCWC 1 cut(s) 793
BslFI GGGAC 2 cut(s) 527, 1729
BslI CCNNNNNNNGG 4 cut(s) 500, 2097, 2098, 2267
BsmAI GTCTC 3 cut(s) 614, 1584, 2431
BsmFI GGGAC 2 cut(s) 527, 1729
BsmI GAATGC 2 cut(s) 512, 526
BsnI GGCC 7 cut(s) 119, 371, 402, 728, 1170, 1892, 2260
Bso31I GGTCTC 1 cut(s) 1584
Bsp1286I GDGCHC 2 cut(s) 793, 1348
Bsp19I CCATGG 1 cut(s) 1380
Bsp68I TCGCGA 1 cut(s) 1848
BspACI CCGC 6 cut(s) 242, 322, 1188, 2180, 2223, 2225
BspANI GGCC 7 cut(s) 119, 371, 402, 728, 1170, 1892, 2260
BspCNI CTCAG 7 cut(s) 354, 842, 990, 1175, 2110, 2217, 2482
BspDI ATCGAT 1 cut(s) 102
BspFNI CGCG 2 cut(s) 1848, 2225
BspLI GGNNCC 6 cut(s) 320, 693, 1243, 1347, 1366, 1717
BspMAI CTGCAG 2 cut(s) 784, 1960
BspPI GGATC 8 cut(s) 313, 326, 833, 1261, 1798, 2078, 2162, 2321
BspT107I GGYRCC 1 cut(s) 1241
BspTI CTTAAG 1 cut(s) 1756
BspTNI GGTCTC 1 cut(s) 1584
BsrI ACTGG 8 cut(s) 120, 274, 310, 320, 1432, 1825, 1927, 2407
BssECI CCNNGG 9 cut(s) 595, 864, 1174, 1380, 1574, 1893, 2096, 2097, 2223
BssT1I CCWWGG 3 cut(s) 1174, 1380, 1893
Bst2UI CCWGG 5 cut(s) 585, 596, 865, 2098, 2412
Bst4CI ACNGT 7 cut(s) 140, 802, 809, 1249, 1578, 2342, 2418
Bst6I CTCTTC 2 cut(s) 436, 1718
BstAFI CTTAAG 1 cut(s) 1756
BstAPI GCANNNNNTGC 2 cut(s) 788, 1190
BstC8I GCNNGC 4 cut(s) 404, 1186, 1593, 2360
BstDEI CTNAG 9 cut(s) 362, 407, 829, 998, 1162, 1335, 2118, 2204, 2490
BstDSI CCRYGG 3 cut(s) 1380, 1574, 2223
BstEII GGTNACC 1 cut(s) 134
BstF5I GGATG 2 cut(s) 2087, 2443
BstFNI CGCG 2 cut(s) 1848, 2225
BstMAI GTCTC 3 cut(s) 614, 1584, 2431
BstMWI GCNNNNNNNGC 7 cut(s) 788, 1190, 1418, 1898, 2123, 2155, 2222
BstNI CCWGG 5 cut(s) 585, 596, 865, 2098, 2412
BstPI GGTNACC 1 cut(s) 134
BstSCI CCNGG 5 cut(s) 583, 594, 863, 2096, 2410
BstSFI CTRYAG 5 cut(s) 67, 84, 780, 1005, 1956
BstUI CGCG 2 cut(s) 1848, 2225
BstV1I GCAGC 4 cut(s) 50, 761, 1264, 1405
BstV2I GAAGAC 2 cut(s) 267, 2340
BstX2I RGATCY 5 cut(s) 318, 988, 2083, 2167, 2326
BstXI CCANNNNNNTGG 3 cut(s) 398, 1178, 2104
BstYI RGATCY 5 cut(s) 318, 988, 2083, 2167, 2326
Bsu15I ATCGAT 1 cut(s) 102
BsuI GTATCC 1 cut(s) 1970
BsuRI GGCC 7 cut(s) 119, 371, 402, 728, 1170, 1892, 2260
BsuTUI ATCGAT 1 cut(s) 102
BtgI CCRYGG 3 cut(s) 1380, 1574, 2223
BtgZI GCGATG 4 cut(s) 1622, 1829, 1863, 2141
BtsCI GGATG 2 cut(s) 2087, 2443
BtsI GCAGTG 2 cut(s) 1272, 1965
BtsIMutI CAGTG 7 cut(s) 113, 135, 313, 1245, 1272, 1818, 1965
BtuMI TCGCGA 1 cut(s) 1848
Cac8I GCNNGC 4 cut(s) 404, 1186, 1593, 2360
CaiI CAGNNNCTG 1 cut(s) 616
Cfr13I GGNCC 6 cut(s) 647, 1091, 1605, 1716, 1925, 2259
Cfr42I CCGCGG 1 cut(s) 2226
ClaI ATCGAT 1 cut(s) 102
CseI GACGC 1 cut(s) 557
Csp6I GTAC 5 cut(s) 810, 1002, 1393, 1953, 2343
CviAII CATG 6 cut(s) 1341, 1357, 1381, 1888, 1946, 2458
CviQI GTAC 5 cut(s) 810, 1002, 1393, 1953, 2343
DdeI CTNAG 9 cut(s) 362, 407, 829, 998, 1162, 1335, 2118, 2204, 2490
EaeI YGGCCR 4 cut(s) 117, 726, 1168, 1890
Eam1104I CTCTTC 2 cut(s) 436, 1718
EarI CTCTTC 2 cut(s) 436, 1718
EciI GGCGGA 1 cut(s) 311
Eco130I CCWWGG 3 cut(s) 1174, 1380, 1893
Eco24I GRGCYC 1 cut(s) 1348
Eco31I GGTCTC 1 cut(s) 1584
Eco47I GGWCC 5 cut(s) 647, 1091, 1605, 1716, 1925
Eco57I CTGAAG 2 cut(s) 111, 2352
Eco91I GGTNACC 1 cut(s) 134
EcoO109I RGGNCCY 2 cut(s) 1716, 2259
EcoO65I GGTNACC 1 cut(s) 134
EcoRII CCWGG 5 cut(s) 583, 594, 863, 2096, 2410
EcoT14I CCWWGG 3 cut(s) 1174, 1380, 1893
EcoT22I ATGCAT 1 cut(s) 1787
EcoT38I GRGCYC 1 cut(s) 1348
ErhI CCWWGG 3 cut(s) 1174, 1380, 1893
FaeI CATG 6 cut(s) 1344, 1360, 1384, 1891, 1949, 2461
FalI AAGNNNNNCTT 2 cut(s) 180, 212
FaqI GGGAC 2 cut(s) 527, 1729
FatI CATG 6 cut(s) 1340, 1356, 1380, 1887, 1945, 2457
FauI CCCGC 1 cut(s) 2173
FbaI TGATCA 2 cut(s) 697, 1936
FblI GTMKAC 2 cut(s) 87, 1747
Fnu4HI GCNGC 4 cut(s) 64, 750, 1278, 1419
FokI GGATG 2 cut(s) 2074, 2450
FriOI GRGCYC 1 cut(s) 1348
Fsp4HI GCNGC 4 cut(s) 64, 750, 1278, 1419
FspBI CTAG 6 cut(s) 149, 258, 498, 710, 1413, 1797
GluI GCNGC 4 cut(s) 64, 750, 1278, 1419
GsaI CCCAGC 1 cut(s) 1755
GsuI CTGGAG 2 cut(s) 638, 2309
HaeIII GGCC 7 cut(s) 119, 371, 402, 728, 1170, 1892, 2260
HgaI GACGC 1 cut(s) 557
Hin1II CATG 6 cut(s) 1344, 1360, 1384, 1891, 1949, 2461
HincII GTYRAC 3 cut(s) 1015, 2254, 2377
HindII GTYRAC 3 cut(s) 1015, 2254, 2377
HinfI GANTC 9 cut(s) 236, 261, 607, 881, 968, 1687, 1726, 2198, 2284
HpaI GTTAAC 1 cut(s) 1015
HphI GGTGA 6 cut(s) 466, 481, 646, 848, 1835, 2119
Hpy99I CGWCG 1 cut(s) 551
HpyAV CCTTC 9 cut(s) 504, 1104, 1299, 1303, 1510, 1759, 1823, 1995, 2309
HpyCH4III ACNGT 7 cut(s) 140, 802, 809, 1249, 1578, 2342, 2418
HpyF10VI GCNNNNNNNGC 7 cut(s) 788, 1190, 1418, 1898, 2123, 2155, 2222
HpyF3I CTNAG 9 cut(s) 362, 407, 829, 998, 1162, 1335, 2118, 2204, 2490
Hsp92II CATG 6 cut(s) 1344, 1360, 1384, 1891, 1949, 2461
Ksp22I TGATCA 2 cut(s) 697, 1936
KspAI GTTAAC 1 cut(s) 1015
KspI CCGCGG 1 cut(s) 2226
LmnI GCTCC 4 cut(s) 796, 1181, 1351, 2155
Lsp1109I GCAGC 4 cut(s) 50, 761, 1264, 1405
LweI GCATC 4 cut(s) 39, 556, 1608, 1816
MaeI CTAG 6 cut(s) 149, 258, 498, 710, 1413, 1797
MaeIII GTNAC 5 cut(s) 134, 460, 481, 859, 1048
MboII GAAGA 7 cut(s) 267, 423, 1283, 1314, 1735, 2152, 2345
MflI RGATCY 5 cut(s) 318, 988, 2083, 2167, 2326
MhlI GDGCHC 2 cut(s) 793, 1348
MlsI TGGCCA 4 cut(s) 119, 728, 1170, 1892
MluNI TGGCCA 4 cut(s) 119, 728, 1170, 1892
MlyI GAGTC 3 cut(s) 616, 962, 1735
MmeI TCCRAC 3 cut(s) 1207, 1231, 2133
Mox20I TGGCCA 4 cut(s) 119, 728, 1170, 1892
Mph1103I ATGCAT 1 cut(s) 1787
MroXI GAANNNNTTC 1 cut(s) 1369
MscI TGGCCA 4 cut(s) 119, 728, 1170, 1892
MseI TTAA 4 cut(s) 1014, 1197, 1536, 1757
MslI CAYNNNNRTG 5 cut(s) 821, 1176, 1734, 1821, 1848
Msp20I TGGCCA 4 cut(s) 119, 728, 1170, 1892
MspA1I CMGCKG 5 cut(s) 244, 752, 833, 2225, 2479
MspCI CTTAAG 1 cut(s) 1756
MspR9I CCNGG 5 cut(s) 585, 596, 865, 2098, 2412
Mva1269I GAATGC 2 cut(s) 512, 526
MvaI CCWGG 5 cut(s) 585, 596, 865, 2098, 2412
MvnI CGCG 2 cut(s) 1848, 2225
MwoI GCNNNNNNNGC 7 cut(s) 788, 1190, 1418, 1898, 2123, 2155, 2222
NcoI CCATGG 1 cut(s) 1380
NlaIII CATG 6 cut(s) 1344, 1360, 1384, 1891, 1949, 2461
NlaIV GGNNCC 6 cut(s) 320, 693, 1243, 1347, 1366, 1717
NmuCI GTSAC 3 cut(s) 460, 481, 859
NruI TCGCGA 1 cut(s) 1848
NsiI ATGCAT 1 cut(s) 1787
OliI CACNNNNGTG 2 cut(s) 1176, 1821
PasI CCCWGGG 1 cut(s) 2097
PctI GAATGC 2 cut(s) 512, 526
PdmI GAANNNNTTC 1 cut(s) 1369
PfeI GAWTC 6 cut(s) 236, 261, 881, 1687, 2198, 2284
PfoI TCCNGGA 1 cut(s) 2410
PkrI GCNGC 4 cut(s) 65, 751, 1279, 1420
PleI GAGTC 3 cut(s) 615, 962, 1734
PpsI GAGTC 3 cut(s) 615, 962, 1734
PpuMI RGGWCCY 1 cut(s) 1716
Psp5II RGGWCCY 1 cut(s) 1716
Psp6I CCWGG 5 cut(s) 583, 594, 863, 2096, 2410
PspEI GGTNACC 1 cut(s) 134
PspFI CCCAGC 1 cut(s) 1751
PspGI CCWGG 5 cut(s) 583, 594, 863, 2096, 2410
PspN4I GGNNCC 6 cut(s) 320, 693, 1243, 1347, 1366, 1717
PspPI GGNCC 6 cut(s) 647, 1091, 1605, 1716, 1925, 2259
PspPPI RGGWCCY 1 cut(s) 1716
PstI CTGCAG 2 cut(s) 784, 1960
PstNI CAGNNNCTG 1 cut(s) 616
PsuI RGATCY 5 cut(s) 318, 988, 2083, 2167, 2326
PvuII CAGCTG 3 cut(s) 752, 833, 2479
RruI TCGCGA 1 cut(s) 1848
RsaI GTAC 5 cut(s) 811, 1003, 1394, 1954, 2344
RsaNI GTAC 5 cut(s) 810, 1002, 1393, 1953, 2343
RseI CAYNNNNRTG 5 cut(s) 821, 1176, 1734, 1821, 1848
SacII CCGCGG 1 cut(s) 2226
SaqAI TTAA 4 cut(s) 1014, 1197, 1536, 1757
SatI GCNGC 4 cut(s) 64, 750, 1278, 1419
Sau96I GGNCC 6 cut(s) 647, 1091, 1605, 1716, 1925, 2259
ScaI AGTACT 3 cut(s) 1003, 1394, 1954
SchI GAGTC 3 cut(s) 616, 962, 1735
ScrFI CCNGG 5 cut(s) 585, 596, 865, 2098, 2412
SduI GDGCHC 2 cut(s) 793, 1348
SfaNI GCATC 4 cut(s) 39, 556, 1608, 1816
SfcI CTRYAG 5 cut(s) 67, 84, 780, 1005, 1956
Sfr303I CCGCGG 1 cut(s) 2226
SgrBI CCGCGG 1 cut(s) 2226
SinI GGWCC 5 cut(s) 647, 1091, 1605, 1716, 1925
SmiMI CAYNNNNRTG 5 cut(s) 821, 1176, 1734, 1821, 1848
SmlI CTYRAG 5 cut(s) 49, 984, 1139, 1756, 2428
SmoI CTYRAG 5 cut(s) 49, 984, 1139, 1756, 2428
SsiI CCGC 6 cut(s) 242, 322, 1188, 2180, 2223, 2225
SspI AATATT 1 cut(s) 1994
SspMI CTAG 6 cut(s) 149, 258, 498, 710, 1413, 1797
StyD4I CCNGG 5 cut(s) 583, 594, 863, 2096, 2410
StyI CCWWGG 3 cut(s) 1174, 1380, 1893
TaaI ACNGT 7 cut(s) 140, 802, 809, 1249, 1578, 2342, 2418
TaqI TCGA 2 cut(s) 102, 663
TaqII GACCGA 1 cut(s) 1622
TatI WGTACW 3 cut(s) 1001, 1392, 1952
TfiI GAWTC 6 cut(s) 236, 261, 881, 1687, 2198, 2284
Tru1I TTAA 4 cut(s) 1014, 1197, 1536, 1757
Tru9I TTAA 4 cut(s) 1014, 1197, 1536, 1757
TscAI CASTG 7 cut(s) 120, 135, 320, 1252, 1272, 1825, 1965
TseFI GTSAC 3 cut(s) 460, 481, 859
TseI GCWGC 4 cut(s) 63, 749, 1277, 1418
Tsp45I GTSAC 3 cut(s) 460, 481, 859
TspDTI ATGAA 9 cut(s) 455, 495, 839, 1163, 1169, 1373, 1832, 2025, 2144
TspGWI ACGGA 2 cut(s) 1416, 1622
TspRI CASTG 7 cut(s) 120, 135, 320, 1252, 1272, 1825, 1965
Vha464I CTTAAG 1 cut(s) 1756
VpaK11BI GGWCC 5 cut(s) 647, 1091, 1605, 1716, 1925
XapI RAATTY 7 cut(s) 17, 169, 374, 590, 1204, 1532, 2210
XcmI CCANNNNNNNNNTGG 1 cut(s) 127
XmiI GTMKAC 2 cut(s) 87, 1747
XmnI GAANNNNTTC 1 cut(s) 1369
XspI CTAG 6 cut(s) 149, 258, 498, 710, 1413, 1797
ZrmI AGTACT 3 cut(s) 1003, 1394, 1954
Zsp2I ATGCAT 1 cut(s) 1787
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.