AT1G49475

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0000156
Physical Location & Seq
Forward (+)
18312883 .. 18314099
1217 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G49475.1

Sequence Viewer

Length: 573 bp
ATGAGGAATATGCATACTAACCGGAGATCACCGGGTCCAATCACGTCGGCAGCAACTCAACGCCGGCTAAAACCGGAGCCAGAACCGACGGTGAAGAAATTCATCAAGATTATTCTCCTCTCAAGAATCATAGAAAAAATGATGAAAGTTCCGGCTAGATTTGTGAGGTTTGGTCCTAAACTCACTGACAACGTGACTCTCCAGACTCCTGTTGGTTTCAAGAGATCAATTCGGATTAAAAGAATCGGTGATGAGGTTTGGTTCGAGAAAGGTTGGAGTGAGTTTGCAGAGGCTCACTCTTTAAGTGATGGTCACTTTCTGTTTTTTCATTACGAAGGGGATTCTTGTTTCCGTGTTGTGATCTTTGATGTGTCTGCTTCTGAGATTGAATATCCATTGGATGATACTGATGATAATAGAGAAGAAGTTATGGATGATGATGAACAAGGGTTTACTGGTTTTGAAAGTAGTGATGATGATGGTGAAGTTGTTGATATGGATGAATTGTTGAAGAAGAAGAAGAAGAAACCAAGAGTCAACATTAAGTCTGAGAATGTCATAATACTTGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

190

Amino Acids

22.0

Weight (kDa)

5.69

Isoelectric Point (pI)

53.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 43 - 123 1.9e-13 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000156)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G49475 AT3G18960 AT3G18960 AT3G18960 AT4G01580
fragaria_vesca FvH4_1g14650 FvH4_1g14650 FvH4_1g14650 FvH4_1g14650 FvH4_1g14660 FvH4_1g14660 FvH4_1g14660 FvH4_1g14660 FvH4_1g14670 FvH4_1g14680 FvH4_1g14680 FvH4_1g14680 FvH4_1g14680 FvH4_1g14680 FvH4_1g14680 FvH4_1g14690 FvH4_1g14700 FvH4_1g29250 FvH4_6g29400 FvH4_6g38681 FvH4_6g38690 FvH4_6g38700 FvH4_6g38700 FvH4_6g38700 FvH4_6g38700 FvH4_6g38700 FvH4_6g38700 FvH4_6g38700 FvH4_7g02010
malus_domestica MD00G1020600.v1.1 MD00G1022000.v1.1 MD00G1022100.v1.1 MD02G1159700.v1.1 MD02G1159800.v1.1 MD02G1159900.v1.1 MD02G1160000.v1.1 MD02G1160300.v1.1 MD15G1274100.v1.1 MD15G1274200.v1.1 MD15G1274400.v1.1 MD15G1302000.v1.1
prunus_persica Prupe.1G579400_v2.0.a1 Prupe.1G579400_v2.0.a1 Prupe.1G579400_v2.0.a1 Prupe.1G579400_v2.0.a1 Prupe.3G083400_v2.0.a1 Prupe.6G229600_v2.0.a1 Prupe.7G039600_v2.0.a1 Prupe.7G044500_v2.0.a1 Prupe.7G044800_v2.0.a1 Prupe.7G143100_v2.0.a1 Prupe.7G143500_v2.0.a1 Prupe.7G143600_v2.0.a1 Prupe.7G143700_v2.0.a1 Prupe.7G143800_v2.0.a1 Prupe.7G143900_v2.0.a1 Prupe.7G143900_v2.0.a1 Prupe.7G143900_v2.0.a1 Prupe.7G143900_v2.0.a1 Prupe.8G072800_v2.0.a1 Prupe.8G072800_v2.0.a1
pyrus_communis pycom02g12620 pycom02g12630 pycom15g23750 pycom15g26490 pycom17g21160
rosa_chinensis RchiOBHm_Chr2g0103421 RchiOBHm_Chr2g0103431 RchiOBHm_Chr2g0103441 RchiOBHm_Chr2g0103491 RchiOBHm_Chr2g0103501 RchiOBHm_Chr2g0103521 RchiOBHm_Chr2g0103541 RchiOBHm_Chr2g0103551 RchiOBHm_Chr2g0103561 RchiOBHm_Chr2g0134821 RchiOBHm_Chr2g0152161 RchiOBHm_Chr2g0152181 RchiOBHm_Chr7g0227261 RchiOBHm_Chr7g0227351 RchiOBHm_Chr7g0227431
rosa_laevigata RLG00000001649 RLG00000001653 RLG00000001659 RLG00000017235 RLG00000017236 RLG00000017240 RLG00000017241 RLG00000017242 RLG00000017243 RLG00000017244 RLG00000017349 RLG00000019424 RLG00000020614 RLG00000020615
rosa_multiflora Rmu_co8247875.1_g000001 Rmu_co8429551.1_g000001 Rmu_sc0000173.1_g000009 Rmu_sc0000718.1_g000004 Rmu_sc0001530.1_g000001 Rmu_sc0001530.1_g000002 Rmu_sc0001530.1_g000004 Rmu_sc0001530.1_g000005 Rmu_sc0001852.1_g000023 Rmu_sc0001852.1_g000024 Rmu_sc0002434.1_g000001 Rmu_sc0002434.1_g000002 Rmu_sc0003043.1_g000016 Rmu_sc0003153.1_g000005 Rmu_sc0011039.1_g000001 Rmu_sc0031822.1_g000001
rosa_roxburghii Rroxscaffold_2G00095860 Rroxscaffold_2G00095870 Rroxscaffold_2G00109720 Rroxscaffold_2G00139530 Rroxscaffold_2G00139550 Rroxscaffold_2G00139570 Rroxscaffold_2G00139580 Rroxscaffold_2G00139630 Rroxscaffold_2G00139640 Rroxscaffold_4G00328430 Rroxscaffold_4G00328440 Rroxscaffold_4G00328450
rosa_rugosa Rorug02G0114900 Rorug02G0115000 Rorug02G0115000 Rorug02G0115200 Rorug02G0115300 Rorug02G0115400 Rorug02G0115500 Rorug02G0115600 Rorug02G0115600 Rorug02G0115600 Rorug02G0115700 Rorug02G0320300 Rorug02G0431300 Rorug02G0431300 Rorug06G0028200 Rorug07G0241300
rosa_samantha Rh2AG163200 Rh2AG163300 Rh2AG163700 Rh2AG163800 Rh2AG163900 Rh2AG164000 Rh2AG373400 Rh2AG492600 Rh2BG170300 Rh2BG170400 Rh2BG170800 Rh2BG170900 Rh2BG171000 Rh2BG171100 Rh2BG171200 Rh2BG377800 Rh2BG505000 Rh2BG505100 Rh2BG505200 Rh2CG170300 Rh2CG170700 Rh2CG479000 Rh2DG169100 Rh2DG169200 Rh2DG169300 Rh2DG516400 Rh7AG391200 Rh7BG376200 Rh7CG410200 Rh7CG411500 Rh7CG412000 Rh7CG412700 Rh7DG388800
rosa_wichuraiana Rw2G012830 Rw2G012840 Rw2G012870 Rw2G012880 Rw2G012890 Rw2G012900 Rw2G030310 Rw2G040470 Rw2G040480 Rw7G032720 Rw7G032730 Rw7G032750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 98
AgsI TTSAA 4 cut(s) 220, 389, 464, 511
AjiI CACGTC 1 cut(s) 45
ApeKI GCWGC 1 cut(s) 50
ApoI RAATTY 1 cut(s) 98
Asp700I GAANNNNTTC 1 cut(s) 98
AspS9I GGNCC 2 cut(s) 35, 173
AsuC2I CCSGG 1 cut(s) 33
AsuHPI GGTGA 4 cut(s) 21, 103, 260, 494
AvaII GGWCC 2 cut(s) 35, 173
BbvI GCAGC 1 cut(s) 62
BccI CCATC 2 cut(s) 302, 473
BcnI CCSGG 1 cut(s) 33
BfaI CTAG 2 cut(s) 156, 571
BisI GCNGC 1 cut(s) 51
BlsI GCNGC 1 cut(s) 52
Bme1390I CCNGG 1 cut(s) 33
Bme18I GGWCC 2 cut(s) 35, 173
BmgBI CACGTC 1 cut(s) 45
BmgT120I GGNCC 2 cut(s) 35, 173
BmiI GGNNCC 2 cut(s) 36, 78
BmrFI CCNGG 1 cut(s) 33
BplI GAGNNNNNCTC 2 cut(s) 281, 313
BpmI CTGGAG 1 cut(s) 185
BpuEI CTTGAG 1 cut(s) 106
BpuMI CCSGG 1 cut(s) 33
BsaWI WCCGGW 2 cut(s) 21, 73
Bse118I RCCGGY 1 cut(s) 63
Bse1I ACTGG 1 cut(s) 460
BseGI GGATG 3 cut(s) 406, 439, 505
BseMII CTCAG 2 cut(s) 372, 540
BseNI ACTGG 1 cut(s) 460
BseRI GAGGAG 1 cut(s) 107
BseXI GCAGC 1 cut(s) 62
BsiSI CCGG 5 cut(s) 22, 32, 64, 74, 152
Bsp143I GATC 3 cut(s) 26, 224, 360
BspCNI CTCAG 2 cut(s) 373, 541
BspLI GGNNCC 2 cut(s) 36, 78
BsrFI RCCGGY 1 cut(s) 63
BsrI ACTGG 1 cut(s) 460
BssAI RCCGGY 1 cut(s) 63
BssMI GATC 3 cut(s) 26, 224, 360
Bst4CI ACNGT 1 cut(s) 91
BstC8I GCNNGC 1 cut(s) 65
BstDEI CTNAG 2 cut(s) 381, 549
BstF5I GGATG 3 cut(s) 406, 439, 505
BstKTI GATC 3 cut(s) 29, 227, 363
BstMBI GATC 3 cut(s) 26, 224, 360
BstSCI CCNGG 1 cut(s) 31
BstV1I GCAGC 1 cut(s) 62
BtrI CACGTC 1 cut(s) 45
BtsCI GGATG 3 cut(s) 406, 439, 505
BtsIMutI CAGTG 1 cut(s) 183
Cac8I GCNNGC 1 cut(s) 65
Cfr10I RCCGGY 1 cut(s) 63
Cfr13I GGNCC 2 cut(s) 35, 173
CviJI RGCY 4 cut(s) 67, 79, 155, 293
CviKI_1 RGCY 4 cut(s) 67, 79, 155, 293
DdeI CTNAG 2 cut(s) 381, 549
DpnI GATC 3 cut(s) 28, 226, 362
DpnII GATC 3 cut(s) 26, 224, 360
Eco47I GGWCC 2 cut(s) 35, 173
EcoT22I ATGCAT 1 cut(s) 15
FaiI YATR 6 cut(s) 11, 15, 131, 431, 497, 560
Fnu4HI GCNGC 1 cut(s) 51
FokI GGATG 3 cut(s) 413, 446, 512
Fsp4HI GCNGC 1 cut(s) 51
FspBI CTAG 2 cut(s) 156, 571
GluI GCNGC 1 cut(s) 51
GsuI CTGGAG 1 cut(s) 185
HapII CCGG 5 cut(s) 22, 32, 64, 74, 152
HincII GTYRAC 1 cut(s) 538
HindII GTYRAC 1 cut(s) 538
HinfI GANTC 6 cut(s) 126, 196, 205, 243, 341, 534
HpaII CCGG 5 cut(s) 22, 32, 64, 74, 152
HphI GGTGA 4 cut(s) 21, 103, 260, 494
Hpy166II GTNNAC 2 cut(s) 453, 538
Hpy188I TCNGA 3 cut(s) 234, 382, 550
Hpy188III TCNNGA 5 cut(s) 106, 123, 202, 220, 265
Hpy8I GTNNAC 2 cut(s) 453, 538
Hpy99I CGWCG 2 cut(s) 49, 91
HpyAV CCTTC 1 cut(s) 329
HpyCH4III ACNGT 1 cut(s) 91
HpyCH4IV ACGT 2 cut(s) 44, 192
HpyCH4V TGCA 2 cut(s) 13, 287
HpyF3I CTNAG 2 cut(s) 381, 549
HpySE526I ACGT 2 cut(s) 44, 192
KroI GCCGGC 1 cut(s) 63
KroNI GCCGGC 1 cut(s) 65
Kzo9I GATC 3 cut(s) 26, 224, 360
LmnI GCTCC 1 cut(s) 76
LpnPI CCDG 9 cut(s) 35, 45, 77, 87, 93, 165, 215, 222, 441
Lsp1109I GCAGC 1 cut(s) 62
MaeI CTAG 2 cut(s) 156, 571
MaeII ACGT 2 cut(s) 44, 192
MaeIII GTNAC 2 cut(s) 193, 311
MalI GATC 3 cut(s) 28, 226, 362
MboI GATC 3 cut(s) 26, 224, 360
MboII GAAGA 7 cut(s) 106, 434, 523, 526, 529, 532, 535
MluCI AATT 3 cut(s) 98, 228, 503
MlyI GAGTC 3 cut(s) 190, 199, 543
MmeI TCCRAC 1 cut(s) 254
MnlI CCTC 4 cut(s) 128, 159, 247, 283
Mph1103I ATGCAT 1 cut(s) 15
MroNI GCCGGC 1 cut(s) 63
MroXI GAANNNNTTC 1 cut(s) 98
MseI TTAA 3 cut(s) 237, 302, 543
MspI CCGG 5 cut(s) 22, 32, 64, 74, 152
MspR9I CCNGG 1 cut(s) 33
NaeI GCCGGC 1 cut(s) 65
NciI CCSGG 1 cut(s) 33
NdeII GATC 3 cut(s) 26, 224, 360
NgoMIV GCCGGC 1 cut(s) 63
NlaIV GGNNCC 2 cut(s) 36, 78
NmuCI GTSAC 2 cut(s) 193, 311
NsiI ATGCAT 1 cut(s) 15
PdiI GCCGGC 1 cut(s) 65
PdmI GAANNNNTTC 1 cut(s) 98
PfeI GAWTC 3 cut(s) 126, 243, 341
PkrI GCNGC 1 cut(s) 52
PleI GAGTC 3 cut(s) 190, 199, 542
PpsI GAGTC 3 cut(s) 190, 199, 542
PspN4I GGNNCC 2 cut(s) 36, 78
PspPI GGNCC 2 cut(s) 35, 173
SaqAI TTAA 3 cut(s) 237, 302, 543
SatI GCNGC 1 cut(s) 51
Sau3AI GATC 3 cut(s) 26, 224, 360
Sau96I GGNCC 2 cut(s) 35, 173
SchI GAGTC 3 cut(s) 190, 199, 543
ScrFI CCNGG 1 cut(s) 33
SetI ASST 5 cut(s) 47, 170, 195, 258, 274
SinI GGWCC 2 cut(s) 35, 173
SmlI CTYRAG 1 cut(s) 121
SmoI CTYRAG 1 cut(s) 121
Sse9I AATT 3 cut(s) 98, 228, 503
SspMI CTAG 2 cut(s) 156, 571
StyD4I CCNGG 1 cut(s) 31
TaaI ACNGT 1 cut(s) 91
TaiI ACGT 2 cut(s) 47, 195
TaqI TCGA 1 cut(s) 264
TasI AATT 3 cut(s) 98, 228, 503
TfiI GAWTC 3 cut(s) 126, 243, 341
Tru1I TTAA 3 cut(s) 237, 302, 543
Tru9I TTAA 3 cut(s) 237, 302, 543
TscAI CASTG 1 cut(s) 190
TseFI GTSAC 2 cut(s) 193, 311
TseI GCWGC 1 cut(s) 50
Tsp45I GTSAC 2 cut(s) 193, 311
TspDTI ATGAA 5 cut(s) 91, 158, 317, 456, 516
TspGWI ACGGA 1 cut(s) 341
TspRI CASTG 1 cut(s) 190
VpaK11BI GGWCC 2 cut(s) 35, 173
XapI RAATTY 1 cut(s) 98
XcmI CCANNNNNNNNNTGG 1 cut(s) 209
XmnI GAANNNNTTC 1 cut(s) 98
XspI CTAG 2 cut(s) 156, 571
Zsp2I ATGCAT 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.