Rroxscaffold_2G00095870

B3 domain-containing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000156
Physical Location & Seq
Reverse (-)
17219879 .. 17223556
3678 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00095870.1

Sequence Viewer

Length: 1419 bp
ATGGCTTCTCTTCGCCCGCTGAGATTTTGTTCTACCACTCCCCATTTTTTCAAGATCATTCTGGAGGACACTTCTCGACGCAAACTTAAAATTCCGAAGAAATTTGTGATGAAATATGGTAAAGATCTGTCAAATTCAGTATTTCTTAAGCTCCCAAGTGGTTCTGAATGGGAAGTAGAATTAACAAGATGCAAAGGTAAGGTTTTGTTTGAGAAGGGTTGGCCAGAGTTTTCTATGTTTTGTTCTCTAGACTATGGGAGCTTCGTGCTTTTTCAATACGAAGGGAATTCTCATTTTGATGTTTGCACATTTGATATGAGTGCCACGGAGATTGATTATCCCATAACAATGCCCAAGATTGAAGAAGCTGATGAAGATGATGGCTTATCTGTTGAAATCTTGGAGGGTTTTCAACCTAGTCCTAAAACAAGAAGGAAATGTCCATTAGCATGTCCTCCTTACAAGAAAATGAGAACAAGTTTGAGCGGCAAAGCAGACCTGATTTCTGAAATTGAGAACACTCAACCTTGTGACATGTTCGAGAAGCCAATCACAAACAAAGAGTCACATCGCAATAAAGTAGAAGTGGAAAATCTGGAAAATAAAGGTGTAGATGATATGTCGGCAGAGAAAGATGGCAGAGTCCCTGGAGGCTTATCTGGCACTCAAAGGTTCCTAAAGAAAACATCTCACAAGGACATCAAGCTTTTATTAGGTGTTTTCTTTTTCCGCACTTCAGAAGCTACAACTTGGTTCTCGTCTAATGGGGAAAATGTCTTTCTGACTGAAAGAGATGGTGGAAGCTCTTCTAGTTCTCAAACAATCCTAAAGCGAACACATGAGGTTGTTGCAAGGGGGAAGCCATTGACTGCAACTGAGAAAGCTAATGCCTTCAAATCTGATAAACCTTCTTTTAGGATTGCCATGCATCCTTATTCTATCCATCATAATTATGTGTATTTGCCAAATGAATTTACTAAGACATATCTTACAAAGCTGTCTTCTGGCGGTTGGTCACCTTTTGTGAGGGACAATAATTTGAAAGTCGGTGATGTGTGTATCTTTACACTGATTAACTGCACTGAATTAGTATTTGATGTTGTCTTTTTCCCCACTGAAGAGTCTGCAGAGAGCCCCTTGTCAACAGGTCATGTCAGACGAGTAACTGTTCAAGTCAGAGAAATGAAACGCCCCCTAGTTAAAGTTGAATCCGAATGCAGCATGAATTGTAAAATTGGCAAGAACAAAATGTCAAATATTAGTGGGCAGGTTACTCAAAGGCCTTCTTCATCTTTGAAGCTGCAAGCAAGTTTTCATCAAAGAATCCCTTCTTCAAAGTCACCCTGGGGTCAGGCCATACTGTGCATGTTCCAGCCAACTTTGCTAGGAATTTCATCAAACGGGACAAGCAAACAGTGA

Protein Analysis

472

Amino Acids

53.25

Weight (kDa)

8.96

Isoelectric Point (pI)

50.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 16 - 102 1.2e-13 B3 DNA binding domain
B3 PF02362 335 - 367 1.5e-06 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000156)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G49475 AT3G18960 AT3G18960 AT3G18960 AT4G01580
fragaria_vesca FvH4_1g14650 FvH4_1g14650 FvH4_1g14650 FvH4_1g14650 FvH4_1g14660 FvH4_1g14660 FvH4_1g14660 FvH4_1g14660 FvH4_1g14670 FvH4_1g14680 FvH4_1g14680 FvH4_1g14680 FvH4_1g14680 FvH4_1g14680 FvH4_1g14680 FvH4_1g14690 FvH4_1g14700 FvH4_1g29250 FvH4_6g29400 FvH4_6g38681 FvH4_6g38690 FvH4_6g38700 FvH4_6g38700 FvH4_6g38700 FvH4_6g38700 FvH4_6g38700 FvH4_6g38700 FvH4_6g38700 FvH4_7g02010
malus_domestica MD00G1020600.v1.1 MD00G1022000.v1.1 MD00G1022100.v1.1 MD02G1159700.v1.1 MD02G1159800.v1.1 MD02G1159900.v1.1 MD02G1160000.v1.1 MD02G1160300.v1.1 MD15G1274100.v1.1 MD15G1274200.v1.1 MD15G1274400.v1.1 MD15G1302000.v1.1
prunus_persica Prupe.1G579400_v2.0.a1 Prupe.1G579400_v2.0.a1 Prupe.1G579400_v2.0.a1 Prupe.1G579400_v2.0.a1 Prupe.3G083400_v2.0.a1 Prupe.6G229600_v2.0.a1 Prupe.7G039600_v2.0.a1 Prupe.7G044500_v2.0.a1 Prupe.7G044800_v2.0.a1 Prupe.7G143100_v2.0.a1 Prupe.7G143500_v2.0.a1 Prupe.7G143600_v2.0.a1 Prupe.7G143700_v2.0.a1 Prupe.7G143800_v2.0.a1 Prupe.7G143900_v2.0.a1 Prupe.7G143900_v2.0.a1 Prupe.7G143900_v2.0.a1 Prupe.7G143900_v2.0.a1 Prupe.8G072800_v2.0.a1 Prupe.8G072800_v2.0.a1
pyrus_communis pycom02g12620 pycom02g12630 pycom15g23750 pycom15g26490 pycom17g21160
rosa_chinensis RchiOBHm_Chr2g0103421 RchiOBHm_Chr2g0103431 RchiOBHm_Chr2g0103441 RchiOBHm_Chr2g0103491 RchiOBHm_Chr2g0103501 RchiOBHm_Chr2g0103521 RchiOBHm_Chr2g0103541 RchiOBHm_Chr2g0103551 RchiOBHm_Chr2g0103561 RchiOBHm_Chr2g0134821 RchiOBHm_Chr2g0152161 RchiOBHm_Chr2g0152181 RchiOBHm_Chr7g0227261 RchiOBHm_Chr7g0227351 RchiOBHm_Chr7g0227431
rosa_laevigata RLG00000001649 RLG00000001653 RLG00000001659 RLG00000017235 RLG00000017236 RLG00000017240 RLG00000017241 RLG00000017242 RLG00000017243 RLG00000017244 RLG00000017349 RLG00000019424 RLG00000020614 RLG00000020615
rosa_multiflora Rmu_co8247875.1_g000001 Rmu_co8429551.1_g000001 Rmu_sc0000173.1_g000009 Rmu_sc0000718.1_g000004 Rmu_sc0001530.1_g000001 Rmu_sc0001530.1_g000002 Rmu_sc0001530.1_g000004 Rmu_sc0001530.1_g000005 Rmu_sc0001852.1_g000023 Rmu_sc0001852.1_g000024 Rmu_sc0002434.1_g000001 Rmu_sc0002434.1_g000002 Rmu_sc0003043.1_g000016 Rmu_sc0003153.1_g000005 Rmu_sc0011039.1_g000001 Rmu_sc0031822.1_g000001
rosa_roxburghii Rroxscaffold_2G00095860 Rroxscaffold_2G00095870 Rroxscaffold_2G00109720 Rroxscaffold_2G00139530 Rroxscaffold_2G00139550 Rroxscaffold_2G00139570 Rroxscaffold_2G00139580 Rroxscaffold_2G00139630 Rroxscaffold_2G00139640 Rroxscaffold_4G00328430 Rroxscaffold_4G00328440 Rroxscaffold_4G00328450
rosa_rugosa Rorug02G0114900 Rorug02G0115000 Rorug02G0115000 Rorug02G0115200 Rorug02G0115300 Rorug02G0115400 Rorug02G0115500 Rorug02G0115600 Rorug02G0115600 Rorug02G0115600 Rorug02G0115700 Rorug02G0320300 Rorug02G0431300 Rorug02G0431300 Rorug06G0028200 Rorug07G0241300
rosa_samantha Rh2AG163200 Rh2AG163300 Rh2AG163700 Rh2AG163800 Rh2AG163900 Rh2AG164000 Rh2AG373400 Rh2AG492600 Rh2BG170300 Rh2BG170400 Rh2BG170800 Rh2BG170900 Rh2BG171000 Rh2BG171100 Rh2BG171200 Rh2BG377800 Rh2BG505000 Rh2BG505100 Rh2BG505200 Rh2CG170300 Rh2CG170700 Rh2CG479000 Rh2DG169100 Rh2DG169200 Rh2DG169300 Rh2DG516400 Rh7AG391200 Rh7BG376200 Rh7CG410200 Rh7CG411500 Rh7CG412000 Rh7CG412700 Rh7DG388800
rosa_wichuraiana Rw2G012830 Rw2G012840 Rw2G012870 Rw2G012880 Rw2G012890 Rw2G012900 Rw2G030310 Rw2G040470 Rw2G040480 Rw7G032720 Rw7G032730 Rw7G032750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1258
AccBSI CCGCTC 1 cut(s) 486
AciI CCGC 4 cut(s) 17, 486, 730, 1008
AcoI YGGCCR 1 cut(s) 221
AcsI RAATTY 6 cut(s) 90, 101, 133, 286, 971, 1389
AcuI CTGAAG 2 cut(s) 720, 1137
AflII CTTAAG 1 cut(s) 146
AflIII ACRYGT 1 cut(s) 534
AjnI CCWGG 2 cut(s) 646, 1343
AluBI AGCT 9 cut(s) 151, 261, 368, 706, 743, 804, 884, 997, 1300
AluI AGCT 9 cut(s) 151, 261, 368, 706, 743, 804, 884, 997, 1300
AoxI GGCC 3 cut(s) 221, 1280, 1353
ApeKI GCWGC 2 cut(s) 1218, 1300
ApoI RAATTY 6 cut(s) 90, 101, 133, 286, 971, 1389
ArsI GACNNNNNNTTYG 2 cut(s) 548, 580
Asp700I GAANNNNTTC 2 cut(s) 805, 1327
AsuHPI GGTGA 3 cut(s) 1008, 1061, 1332
BalI TGGCCA 1 cut(s) 223
BanII GRGCYC 1 cut(s) 1136
BbsI GAAGAC 1 cut(s) 993
BbvI GCAGC 2 cut(s) 1230, 1287
BccI CCATC 4 cut(s) 374, 629, 788, 951
BciT130I CCWGG 2 cut(s) 648, 1345
BfaI CTAG 5 cut(s) 248, 417, 810, 1196, 1385
BfmI CTRYAG 1 cut(s) 1125
BfrI CTTAAG 1 cut(s) 146
BfuAI ACCTGC 1 cut(s) 1258
BglII AGATCT 1 cut(s) 124
BisI GCNGC 3 cut(s) 487, 1219, 1301
BlsI GCNGC 3 cut(s) 488, 1220, 1302
Bme1390I CCNGG 2 cut(s) 648, 1345
BmiI GGNNCC 1 cut(s) 674
BmrFI CCNGG 2 cut(s) 648, 1345
BmsI GCATC 2 cut(s) 179, 937
BpiI GAAGAC 1 cut(s) 993
BpmI CTGGAG 2 cut(s) 83, 669
BsaJI CCNNGG 4 cut(s) 324, 646, 1343, 1344
BseBI CCWGG 2 cut(s) 648, 1345
BseDI CCNNGG 4 cut(s) 324, 646, 1343, 1344
BseGI GGATG 1 cut(s) 928
BseMII CTCAG 2 cut(s) 11, 867
BseXI GCAGC 2 cut(s) 1230, 1287
BsgI GTGCAG 1 cut(s) 1063
BshFI GGCC 3 cut(s) 223, 1282, 1355
BslFI GGGAC 2 cut(s) 629, 1043
BsmFI GGGAC 2 cut(s) 629, 1043
BsmI GAATGC 1 cut(s) 1220
BsnI GGCC 3 cut(s) 223, 1282, 1355
Bsp1286I GDGCHC 1 cut(s) 1136
Bsp143I GATC 2 cut(s) 54, 124
BspACI CCGC 4 cut(s) 17, 486, 730, 1008
BspANI GGCC 3 cut(s) 223, 1282, 1355
BspCNI CTCAG 2 cut(s) 12, 868
BspLI GGNNCC 1 cut(s) 674
BspMAI CTGCAG 1 cut(s) 1129
BspMI ACCTGC 1 cut(s) 1258
BspQI GCTCTTC 1 cut(s) 811
BspTI CTTAAG 1 cut(s) 146
BsrBI CCGCTC 1 cut(s) 486
BssECI CCNNGG 4 cut(s) 324, 646, 1343, 1344
BssMI GATC 2 cut(s) 54, 124
Bst2UI CCWGG 2 cut(s) 648, 1345
Bst4CI ACNGT 3 cut(s) 1168, 1362, 1416
Bst6I CTCTTC 3 cut(s) 15, 811, 1113
BstAFI CTTAAG 1 cut(s) 146
BstC8I GCNNGC 2 cut(s) 17, 1305
BstDEI CTNAG 3 cut(s) 20, 876, 978
BstDSI CCRYGG 1 cut(s) 324
BstEII GGTNACC 1 cut(s) 1014
BstF5I GGATG 1 cut(s) 928
BstKTI GATC 2 cut(s) 57, 127
BstMBI GATC 2 cut(s) 54, 124
BstMWI GCNNNNNNNGC 2 cut(s) 660, 1381
BstNI CCWGG 2 cut(s) 648, 1345
BstNSI RCATGY 3 cut(s) 453, 538, 1369
BstPI GGTNACC 1 cut(s) 1014
BstSCI CCNGG 2 cut(s) 646, 1343
BstSFI CTRYAG 1 cut(s) 1125
BstV1I GCAGC 2 cut(s) 1230, 1287
BstV2I GAAGAC 1 cut(s) 993
BstX2I RGATCY 1 cut(s) 124
BstYI RGATCY 1 cut(s) 124
BsuRI GGCC 3 cut(s) 223, 1282, 1355
BtgI CCRYGG 1 cut(s) 324
BtgZI GCGATG 1 cut(s) 554
BtsCI GGATG 1 cut(s) 928
BtsIMutI CAGTG 3 cut(s) 1067, 1080, 1113
BveI ACCTGC 1 cut(s) 1258
Cac8I GCNNGC 2 cut(s) 17, 1305
CseI GACGC 1 cut(s) 87
CspCI CAANNNNNGTGG 2 cut(s) 313, 348
CviAII CATG 7 cut(s) 450, 535, 839, 925, 1151, 1222, 1366
DdeI CTNAG 3 cut(s) 20, 876, 978
DpnI GATC 2 cut(s) 56, 126
DpnII GATC 2 cut(s) 54, 124
EaeI YGGCCR 1 cut(s) 221
Eam1104I CTCTTC 3 cut(s) 15, 811, 1113
EarI CTCTTC 3 cut(s) 15, 811, 1113
Eco147I AGGCCT 1 cut(s) 1282
Eco24I GRGCYC 1 cut(s) 1136
Eco57I CTGAAG 2 cut(s) 720, 1137
Eco91I GGTNACC 1 cut(s) 1014
EcoO65I GGTNACC 1 cut(s) 1014
EcoRI GAATTC 1 cut(s) 286
EcoRII CCWGG 2 cut(s) 646, 1343
EcoT22I ATGCAT 1 cut(s) 930
EcoT38I GRGCYC 1 cut(s) 1136
FaeI CATG 7 cut(s) 453, 538, 842, 928, 1154, 1225, 1369
FalI AAGNNNNNCTT 4 cut(s) 1270, 1302, 1312, 1344
FaqI GGGAC 2 cut(s) 629, 1043
FatI CATG 7 cut(s) 449, 534, 838, 924, 1150, 1221, 1365
FauI CCCGC 1 cut(s) 24
Fnu4HI GCNGC 3 cut(s) 487, 1219, 1301
FokI GGATG 1 cut(s) 915
FriOI GRGCYC 1 cut(s) 1136
Fsp4HI GCNGC 3 cut(s) 487, 1219, 1301
FspBI CTAG 5 cut(s) 248, 417, 810, 1196, 1385
GluI GCNGC 3 cut(s) 487, 1219, 1301
GsuI CTGGAG 2 cut(s) 83, 669
HaeIII GGCC 3 cut(s) 223, 1282, 1355
HgaI GACGC 1 cut(s) 87
Hin1II CATG 7 cut(s) 453, 538, 842, 928, 1154, 1225, 1369
HincII GTYRAC 1 cut(s) 1143
HindII GTYRAC 1 cut(s) 1143
HindIII AAGCTT 1 cut(s) 704
HinfI GANTC 5 cut(s) 563, 642, 1121, 1208, 1323
HphI GGTGA 3 cut(s) 1008, 1061, 1332
Hpy166II GTNNAC 1 cut(s) 1143
Hpy188I TCNGA 9 cut(s) 96, 166, 508, 739, 783, 901, 1157, 1178, 1213
Hpy188III TCNNGA 6 cut(s) 52, 62, 75, 248, 541, 596
Hpy8I GTNNAC 1 cut(s) 1143
Hpy99I CGWCG 1 cut(s) 81
HpyAV CCTTC 7 cut(s) 208, 275, 426, 901, 918, 1293, 1338
HpyCH4III ACNGT 3 cut(s) 1168, 1362, 1416
HpyF10VI GCNNNNNNNGC 2 cut(s) 660, 1381
HpyF3I CTNAG 3 cut(s) 20, 876, 978
Hsp92II CATG 7 cut(s) 453, 538, 842, 928, 1154, 1225, 1369
Kzo9I GATC 2 cut(s) 54, 124
LguI GCTCTTC 1 cut(s) 811
LmnI GCTCC 2 cut(s) 156, 258
Lsp1109I GCAGC 2 cut(s) 1230, 1287
LweI GCATC 2 cut(s) 179, 937
MaeI CTAG 5 cut(s) 248, 417, 810, 1196, 1385
MaeIII GTNAC 6 cut(s) 530, 564, 1014, 1162, 1270, 1338
MalI GATC 2 cut(s) 56, 126
MbiI CCGCTC 1 cut(s) 486
MboI GATC 2 cut(s) 54, 124
MboII GAAGA 8 cut(s) 109, 374, 386, 798, 993, 1130, 1278, 1323
MflI RGATCY 1 cut(s) 124
MhlI GDGCHC 1 cut(s) 1136
MlsI TGGCCA 1 cut(s) 223
MluNI TGGCCA 1 cut(s) 223
MlyI GAGTC 3 cut(s) 572, 651, 1130
MnlI CCTC 6 cut(s) 58, 397, 465, 644, 835, 1020
Mox20I TGGCCA 1 cut(s) 223
Mph1103I ATGCAT 1 cut(s) 930
MroXI GAANNNNTTC 2 cut(s) 805, 1327
MscI TGGCCA 1 cut(s) 223
MseI TTAA 5 cut(s) 87, 147, 182, 1074, 1200
MslI CAYNNNNRTG 4 cut(s) 297, 347, 448, 951
Msp20I TGGCCA 1 cut(s) 223
MspA1I CMGCKG 1 cut(s) 19
MspCI CTTAAG 1 cut(s) 146
MspR9I CCNGG 2 cut(s) 648, 1345
Mva1269I GAATGC 1 cut(s) 1220
MvaI CCWGG 2 cut(s) 648, 1345
MwoI GCNNNNNNNGC 2 cut(s) 660, 1381
NdeII GATC 2 cut(s) 54, 124
NlaIII CATG 7 cut(s) 453, 538, 842, 928, 1154, 1225, 1369
NlaIV GGNNCC 1 cut(s) 674
NmuCI GTSAC 4 cut(s) 530, 564, 1014, 1338
NsiI ATGCAT 1 cut(s) 930
NspI RCATGY 3 cut(s) 453, 538, 1369
PasI CCCWGGG 1 cut(s) 1344
PceI AGGCCT 1 cut(s) 1282
PciI ACATGT 1 cut(s) 534
PciSI GCTCTTC 1 cut(s) 811
PctI GAATGC 1 cut(s) 1220
PdmI GAANNNNTTC 2 cut(s) 805, 1327
PfeI GAWTC 2 cut(s) 1208, 1323
PkrI GCNGC 3 cut(s) 488, 1220, 1302
PleI GAGTC 3 cut(s) 571, 650, 1129
PpsI GAGTC 3 cut(s) 571, 650, 1129
PscI ACATGT 1 cut(s) 534
Psp6I CCWGG 2 cut(s) 646, 1343
PspEI GGTNACC 1 cut(s) 1014
PspGI CCWGG 2 cut(s) 646, 1343
PspN4I GGNNCC 1 cut(s) 674
PstI CTGCAG 1 cut(s) 1129
PsuI RGATCY 1 cut(s) 124
RseI CAYNNNNRTG 4 cut(s) 297, 347, 448, 951
SapI GCTCTTC 1 cut(s) 811
SaqAI TTAA 5 cut(s) 87, 147, 182, 1074, 1200
SatI GCNGC 3 cut(s) 487, 1219, 1301
Sau3AI GATC 2 cut(s) 54, 124
SchI GAGTC 3 cut(s) 572, 651, 1130
ScrFI CCNGG 2 cut(s) 648, 1345
SduI GDGCHC 1 cut(s) 1136
SfaNI GCATC 2 cut(s) 179, 937
SfcI CTRYAG 1 cut(s) 1125
SmiMI CAYNNNNRTG 4 cut(s) 297, 347, 448, 951
SmlI CTYRAG 1 cut(s) 146
SmoI CTYRAG 1 cut(s) 146
SseBI AGGCCT 1 cut(s) 1282
SsiI CCGC 4 cut(s) 17, 486, 730, 1008
SspI AATATT 1 cut(s) 1258
SspMI CTAG 5 cut(s) 248, 417, 810, 1196, 1385
StuI AGGCCT 1 cut(s) 1282
StyD4I CCNGG 2 cut(s) 646, 1343
TaaI ACNGT 3 cut(s) 1168, 1362, 1416
TaqI TCGA 2 cut(s) 76, 540
TauI GCSGC 1 cut(s) 489
TfiI GAWTC 2 cut(s) 1208, 1323
Tru1I TTAA 5 cut(s) 87, 147, 182, 1074, 1200
Tru9I TTAA 5 cut(s) 87, 147, 182, 1074, 1200
TscAI CASTG 3 cut(s) 1074, 1087, 1120
TseFI GTSAC 4 cut(s) 530, 564, 1014, 1338
TseI GCWGC 2 cut(s) 1218, 1300
Tsp45I GTSAC 4 cut(s) 530, 564, 1014, 1338
TspDTI ATGAA 8 cut(s) 125, 387, 984, 1199, 1238, 1278, 1304, 1383
TspGWI ACGGA 1 cut(s) 341
TspRI CASTG 3 cut(s) 1074, 1087, 1120
Vha464I CTTAAG 1 cut(s) 146
XapI RAATTY 6 cut(s) 90, 101, 133, 286, 971, 1389
XbaI TCTAGA 1 cut(s) 247
XceI RCATGY 3 cut(s) 453, 538, 1369
XmnI GAANNNNTTC 2 cut(s) 805, 1327
XspI CTAG 5 cut(s) 248, 417, 810, 1196, 1385
Zsp2I ATGCAT 1 cut(s) 930
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.