B3 domain-containing
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 711 |
| AccB1I | GGYRCC | 1 cut(s) | 257 |
| AciI | CCGC | 1 cut(s) | 192 |
| AclWI | GGATC | 2 cut(s) | 145, 951 |
| AcsI | RAATTY | 9 cut(s) | 90, 101, 133, 236, 286, 293, 502, 814, 842 |
| AcuI | CTGAAG | 1 cut(s) | 764 |
| AfaI | GTAC | 1 cut(s) | 726 |
| AflII | CTTAAG | 1 cut(s) | 146 |
| AgsI | TTSAA | 9 cut(s) | 49, 395, 489, 509, 641, 658, 721, 814, 834 |
| AhlI | ACTAGT | 1 cut(s) | 317 |
| AjnI | CCWGG | 2 cut(s) | 297, 399 |
| AluBI | AGCT | 7 cut(s) | 617, 635, 647, 769, 778, 839, 913 |
| AluI | AGCT | 7 cut(s) | 617, 635, 647, 769, 778, 839, 913 |
| Alw21I | GWGCWC | 1 cut(s) | 915 |
| Alw26I | GTCTC | 1 cut(s) | 69 |
| AlwI | GGATC | 2 cut(s) | 145, 951 |
| AoxI | GGCC | 3 cut(s) | 14, 221, 800 |
| ApoI | RAATTY | 9 cut(s) | 90, 101, 133, 236, 286, 293, 502, 814, 842 |
| AseI | ATTAAT | 1 cut(s) | 369 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 484, 504 |
| AsuHPI | GGTGA | 1 cut(s) | 676 |
| AsuII | TTCGAA | 1 cut(s) | 571 |
| BanI | GGYRCC | 1 cut(s) | 257 |
| BanII | GRGCYC | 1 cut(s) | 915 |
| Bbv12I | GWGCWC | 1 cut(s) | 915 |
| BccI | CCATC | 4 cut(s) | 350, 460, 849, 884 |
| BciT130I | CCWGG | 2 cut(s) | 299, 401 |
| BcoDI | GTCTC | 1 cut(s) | 69 |
| BcuI | ACTAGT | 1 cut(s) | 317 |
| BfaI | CTAG | 4 cut(s) | 72, 248, 318, 555 |
| BfmI | CTRYAG | 1 cut(s) | 630 |
| BfrI | CTTAAG | 1 cut(s) | 146 |
| BglII | AGATCT | 1 cut(s) | 794 |
| BisI | GCNGC | 1 cut(s) | 193 |
| BlsI | GCNGC | 1 cut(s) | 194 |
| Bme1390I | CCNGG | 2 cut(s) | 299, 401 |
| BmiI | GGNNCC | 1 cut(s) | 259 |
| BmrFI | CCNGG | 2 cut(s) | 299, 401 |
| BmsI | GCATC | 1 cut(s) | 591 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 111, 143 |
| BpmI | CTGGAG | 1 cut(s) | 80 |
| Bpu14I | TTCGAA | 1 cut(s) | 571 |
| Bse1I | ACTGG | 1 cut(s) | 732 |
| BseBI | CCWGG | 2 cut(s) | 299, 401 |
| BseGI | GGATG | 4 cut(s) | 342, 606, 691, 860 |
| BseNI | ACTGG | 1 cut(s) | 732 |
| BshFI | GGCC | 3 cut(s) | 16, 223, 802 |
| BshNI | GGYRCC | 1 cut(s) | 257 |
| BsiHKAI | GWGCWC | 1 cut(s) | 915 |
| BsmAI | GTCTC | 1 cut(s) | 69 |
| BsmI | GAATGC | 2 cut(s) | 227, 604 |
| BsnI | GGCC | 3 cut(s) | 16, 223, 802 |
| Bsp119I | TTCGAA | 1 cut(s) | 571 |
| Bsp1286I | GDGCHC | 1 cut(s) | 915 |
| Bsp143I | GATC | 4 cut(s) | 51, 150, 794, 956 |
| BspACI | CCGC | 1 cut(s) | 192 |
| BspANI | GGCC | 3 cut(s) | 16, 223, 802 |
| BspLI | GGNNCC | 1 cut(s) | 259 |
| BspPI | GGATC | 2 cut(s) | 145, 951 |
| BspT104I | TTCGAA | 1 cut(s) | 571 |
| BspT107I | GGYRCC | 1 cut(s) | 257 |
| BspTI | CTTAAG | 1 cut(s) | 146 |
| BsrI | ACTGG | 1 cut(s) | 732 |
| BssMI | GATC | 4 cut(s) | 51, 150, 794, 956 |
| Bst2UI | CCWGG | 2 cut(s) | 299, 401 |
| BstAFI | CTTAAG | 1 cut(s) | 146 |
| BstBI | TTCGAA | 1 cut(s) | 571 |
| BstC8I | GCNNGC | 3 cut(s) | 221, 225, 861 |
| BstF5I | GGATG | 4 cut(s) | 342, 606, 691, 860 |
| BstKTI | GATC | 4 cut(s) | 54, 153, 797, 959 |
| BstMAI | GTCTC | 1 cut(s) | 69 |
| BstMBI | GATC | 4 cut(s) | 51, 150, 794, 956 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 555, 775 |
| BstNI | CCWGG | 2 cut(s) | 299, 401 |
| BstNSI | RCATGY | 1 cut(s) | 863 |
| BstSCI | CCNGG | 2 cut(s) | 297, 399 |
| BstSFI | CTRYAG | 1 cut(s) | 630 |
| BstX2I | RGATCY | 3 cut(s) | 150, 794, 956 |
| BstYI | RGATCY | 3 cut(s) | 150, 794, 956 |
| BsuRI | GGCC | 3 cut(s) | 16, 223, 802 |
| BtsCI | GGATG | 4 cut(s) | 342, 606, 691, 860 |
| Cac8I | GCNNGC | 3 cut(s) | 221, 225, 861 |
| CseI | GACGC | 1 cut(s) | 193 |
| Csp6I | GTAC | 1 cut(s) | 725 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 313, 348 |
| CviAII | CATG | 1 cut(s) | 860 |
| CviQI | GTAC | 1 cut(s) | 725 |
| DpnI | GATC | 4 cut(s) | 53, 152, 796, 958 |
| DpnII | GATC | 4 cut(s) | 51, 150, 794, 956 |
| Ecl136II | GAGCTC | 1 cut(s) | 913 |
| Eco24I | GRGCYC | 1 cut(s) | 915 |
| Eco53kI | GAGCTC | 1 cut(s) | 913 |
| Eco57I | CTGAAG | 1 cut(s) | 764 |
| EcoICRI | GAGCTC | 1 cut(s) | 913 |
| EcoRII | CCWGG | 2 cut(s) | 297, 399 |
| EcoT22I | ATGCAT | 2 cut(s) | 606, 865 |
| EcoT38I | GRGCYC | 1 cut(s) | 915 |
| FaeI | CATG | 1 cut(s) | 863 |
| FatI | CATG | 1 cut(s) | 859 |
| Fnu4HI | GCNGC | 1 cut(s) | 193 |
| FokI | GGATG | 4 cut(s) | 329, 613, 678, 867 |
| FriOI | GRGCYC | 1 cut(s) | 915 |
| Fsp4HI | GCNGC | 1 cut(s) | 193 |
| FspBI | CTAG | 4 cut(s) | 72, 248, 318, 555 |
| GluI | GCNGC | 1 cut(s) | 193 |
| GsuI | CTGGAG | 1 cut(s) | 80 |
| HaeIII | GGCC | 3 cut(s) | 16, 223, 802 |
| HgaI | GACGC | 1 cut(s) | 193 |
| Hin1II | CATG | 1 cut(s) | 863 |
| HincII | GTYRAC | 1 cut(s) | 612 |
| HindII | GTYRAC | 1 cut(s) | 612 |
| HindIII | AAGCTT | 1 cut(s) | 776 |
| HinfI | GANTC | 1 cut(s) | 356 |
| HphI | GGTGA | 1 cut(s) | 676 |
| Hpy166II | GTNNAC | 2 cut(s) | 612, 946 |
| Hpy188I | TCNGA | 3 cut(s) | 361, 530, 664 |
| Hpy188III | TCNNGA | 6 cut(s) | 49, 59, 72, 109, 638, 742 |
| Hpy8I | GTNNAC | 2 cut(s) | 612, 946 |
| HpyAV | CCTTC | 3 cut(s) | 208, 271, 275 |
| HpyCH4IV | ACGT | 2 cut(s) | 20, 930 |
| HpyCH4V | TGCA | 5 cut(s) | 227, 604, 623, 863, 903 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 555, 775 |
| HpySE526I | ACGT | 2 cut(s) | 20, 930 |
| Hsp92II | CATG | 1 cut(s) | 863 |
| Kzo9I | GATC | 4 cut(s) | 51, 150, 794, 956 |
| LweI | GCATC | 1 cut(s) | 591 |
| MaeI | CTAG | 4 cut(s) | 72, 248, 318, 555 |
| MaeII | ACGT | 2 cut(s) | 20, 930 |
| MaeIII | GTNAC | 2 cut(s) | 181, 682 |
| MalI | GATC | 4 cut(s) | 53, 152, 796, 958 |
| MboI | GATC | 4 cut(s) | 51, 150, 794, 956 |
| MboII | GAAGA | 4 cut(s) | 365, 389, 486, 489 |
| MfeI | CAATTG | 1 cut(s) | 877 |
| MflI | RGATCY | 3 cut(s) | 150, 794, 956 |
| MhlI | GDGCHC | 1 cut(s) | 915 |
| MmeI | TCCRAC | 1 cut(s) | 894 |
| MnlI | CCTC | 3 cut(s) | 474, 539, 591 |
| Mph1103I | ATGCAT | 2 cut(s) | 606, 865 |
| MroXI | GAANNNNTTC | 2 cut(s) | 484, 504 |
| MseI | TTAA | 3 cut(s) | 87, 147, 369 |
| MslI | CAYNNNNRTG | 1 cut(s) | 920 |
| MspCI | CTTAAG | 1 cut(s) | 146 |
| MspR9I | CCNGG | 2 cut(s) | 299, 401 |
| MunI | CAATTG | 1 cut(s) | 877 |
| Mva1269I | GAATGC | 2 cut(s) | 227, 604 |
| MvaI | CCWGG | 2 cut(s) | 299, 401 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 555, 775 |
| NdeII | GATC | 4 cut(s) | 51, 150, 794, 956 |
| NlaIII | CATG | 1 cut(s) | 863 |
| NlaIV | GGNNCC | 1 cut(s) | 259 |
| NmuCI | GTSAC | 2 cut(s) | 181, 682 |
| NsiI | ATGCAT | 2 cut(s) | 606, 865 |
| NspI | RCATGY | 1 cut(s) | 863 |
| NspV | TTCGAA | 1 cut(s) | 571 |
| PaeI | GCATGC | 1 cut(s) | 863 |
| PctI | GAATGC | 2 cut(s) | 227, 604 |
| PdmI | GAANNNNTTC | 2 cut(s) | 484, 504 |
| PfeI | GAWTC | 1 cut(s) | 356 |
| PfoI | TCCNGGA | 1 cut(s) | 399 |
| PkrI | GCNGC | 1 cut(s) | 194 |
| PshBI | ATTAAT | 1 cut(s) | 369 |
| PsiI | TTATAA | 1 cut(s) | 711 |
| Psp124BI | GAGCTC | 1 cut(s) | 915 |
| Psp6I | CCWGG | 2 cut(s) | 297, 399 |
| PspGI | CCWGG | 2 cut(s) | 297, 399 |
| PspN4I | GGNNCC | 1 cut(s) | 259 |
| PsuI | RGATCY | 3 cut(s) | 150, 794, 956 |
| RsaI | GTAC | 1 cut(s) | 726 |
| RsaNI | GTAC | 1 cut(s) | 725 |
| RseI | CAYNNNNRTG | 1 cut(s) | 920 |
| SacI | GAGCTC | 1 cut(s) | 915 |
| SaqAI | TTAA | 3 cut(s) | 87, 147, 369 |
| SatI | GCNGC | 1 cut(s) | 193 |
| Sau3AI | GATC | 4 cut(s) | 51, 150, 794, 956 |
| ScrFI | CCNGG | 2 cut(s) | 299, 401 |
| SduI | GDGCHC | 1 cut(s) | 915 |
| SfaNI | GCATC | 1 cut(s) | 591 |
| SfcI | CTRYAG | 1 cut(s) | 630 |
| SfuI | TTCGAA | 1 cut(s) | 571 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 920 |
| SmlI | CTYRAG | 1 cut(s) | 146 |
| SmoI | CTYRAG | 1 cut(s) | 146 |
| SpeI | ACTAGT | 1 cut(s) | 317 |
| SphI | GCATGC | 1 cut(s) | 863 |
| SsiI | CCGC | 1 cut(s) | 192 |
| SspMI | CTAG | 4 cut(s) | 72, 248, 318, 555 |
| SstI | GAGCTC | 1 cut(s) | 915 |
| StyD4I | CCNGG | 2 cut(s) | 297, 399 |
| TaiI | ACGT | 2 cut(s) | 23, 933 |
| TaqI | TCGA | 1 cut(s) | 571 |
| TauI | GCSGC | 1 cut(s) | 195 |
| TfiI | GAWTC | 1 cut(s) | 356 |
| Tru1I | TTAA | 3 cut(s) | 87, 147, 369 |
| Tru9I | TTAA | 3 cut(s) | 87, 147, 369 |
| TseFI | GTSAC | 2 cut(s) | 181, 682 |
| Tsp45I | GTSAC | 2 cut(s) | 181, 682 |
| TspDTI | ATGAA | 3 cut(s) | 294, 680, 938 |
| TspGWI | ACGGA | 2 cut(s) | 684, 949 |
| Vha464I | CTTAAG | 1 cut(s) | 146 |
| VspI | ATTAAT | 1 cut(s) | 369 |
| XapI | RAATTY | 9 cut(s) | 90, 101, 133, 236, 286, 293, 502, 814, 842 |
| XbaI | TCTAGA | 1 cut(s) | 71 |
| XceI | RCATGY | 1 cut(s) | 863 |
| XmnI | GAANNNNTTC | 2 cut(s) | 484, 504 |
| XspI | CTAG | 4 cut(s) | 72, 248, 318, 555 |
| Zsp2I | ATGCAT | 2 cut(s) | 606, 865 |