AT3G18960

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0000156
Physical Location & Seq
Forward (+)
6542456 .. 6543676
1221 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G18960.2

Sequence Viewer

Length: 630 bp
ATGGTTACAACCCAAAACACGAAGGCCAGAGTCACCTCCGTCTCTCACCGCCGATCACAGCAAGATCCTGAGTCGCCGGTGAAGAAGTTCTTCAAGTTGGTCTTACCTTCAACGATGAAGGATAAGATGATGAAGATTCCGCCAAGATTCGTGAAGCTACAAGGGTCTAAACTCTCAGAGGTTGTTACATTAGAGACTCCTGCGGGTTTCAAGCGTTCCATTAAGCTCAAACGGATCGGTGAAGAGATTTGGTTCCATGAGGGTTGGAGTGAGTTTGCAGAGGCTCATTCCATCGAGGAAGGCCACTTTCTCTTGTTCGAATACAAGGAGAATTCGAGTTTCCGTGTAATAATCTTCAATGTTTCGGCTTGCGAGACCAAATATCCACTGGATGCAGTTCATATCATTGACAGCGACGATGATATAATCGATATTACAGGCAAAGAGTTTGTTGGAACTCAAGGTACAAGGAAGAATAATCAATCTGTTAATGGTGGTGATACAGAGCACAAATCCAAGAAGAGATCAAGAGACATCGAACTTGACAAGATTCTACACGATCTTGATGTGATGAATCCAATGCATGTGCTTAAAGAAGAAGAAGAAGACAAGAGAGTCTTCAGGGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

209

Amino Acids

24.09

Weight (kDa)

7.11

Isoelectric Point (pI)

55.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 30 - 120 4.5e-17 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000156)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G49475 AT3G18960 AT3G18960 AT3G18960 AT4G01580
fragaria_vesca FvH4_1g14650 FvH4_1g14650 FvH4_1g14650 FvH4_1g14650 FvH4_1g14660 FvH4_1g14660 FvH4_1g14660 FvH4_1g14660 FvH4_1g14670 FvH4_1g14680 FvH4_1g14680 FvH4_1g14680 FvH4_1g14680 FvH4_1g14680 FvH4_1g14680 FvH4_1g14690 FvH4_1g14700 FvH4_1g29250 FvH4_6g29400 FvH4_6g38681 FvH4_6g38690 FvH4_6g38700 FvH4_6g38700 FvH4_6g38700 FvH4_6g38700 FvH4_6g38700 FvH4_6g38700 FvH4_6g38700 FvH4_7g02010
malus_domestica MD00G1020600.v1.1 MD00G1022000.v1.1 MD00G1022100.v1.1 MD02G1159700.v1.1 MD02G1159800.v1.1 MD02G1159900.v1.1 MD02G1160000.v1.1 MD02G1160300.v1.1 MD15G1274100.v1.1 MD15G1274200.v1.1 MD15G1274400.v1.1 MD15G1302000.v1.1
prunus_persica Prupe.1G579400_v2.0.a1 Prupe.1G579400_v2.0.a1 Prupe.1G579400_v2.0.a1 Prupe.1G579400_v2.0.a1 Prupe.3G083400_v2.0.a1 Prupe.6G229600_v2.0.a1 Prupe.7G039600_v2.0.a1 Prupe.7G044500_v2.0.a1 Prupe.7G044800_v2.0.a1 Prupe.7G143100_v2.0.a1 Prupe.7G143500_v2.0.a1 Prupe.7G143600_v2.0.a1 Prupe.7G143700_v2.0.a1 Prupe.7G143800_v2.0.a1 Prupe.7G143900_v2.0.a1 Prupe.7G143900_v2.0.a1 Prupe.7G143900_v2.0.a1 Prupe.7G143900_v2.0.a1 Prupe.8G072800_v2.0.a1 Prupe.8G072800_v2.0.a1
pyrus_communis pycom02g12620 pycom02g12630 pycom15g23750 pycom15g26490 pycom17g21160
rosa_chinensis RchiOBHm_Chr2g0103421 RchiOBHm_Chr2g0103431 RchiOBHm_Chr2g0103441 RchiOBHm_Chr2g0103491 RchiOBHm_Chr2g0103501 RchiOBHm_Chr2g0103521 RchiOBHm_Chr2g0103541 RchiOBHm_Chr2g0103551 RchiOBHm_Chr2g0103561 RchiOBHm_Chr2g0134821 RchiOBHm_Chr2g0152161 RchiOBHm_Chr2g0152181 RchiOBHm_Chr7g0227261 RchiOBHm_Chr7g0227351 RchiOBHm_Chr7g0227431
rosa_laevigata RLG00000001649 RLG00000001653 RLG00000001659 RLG00000017235 RLG00000017236 RLG00000017240 RLG00000017241 RLG00000017242 RLG00000017243 RLG00000017244 RLG00000017349 RLG00000019424 RLG00000020614 RLG00000020615
rosa_multiflora Rmu_co8247875.1_g000001 Rmu_co8429551.1_g000001 Rmu_sc0000173.1_g000009 Rmu_sc0000718.1_g000004 Rmu_sc0001530.1_g000001 Rmu_sc0001530.1_g000002 Rmu_sc0001530.1_g000004 Rmu_sc0001530.1_g000005 Rmu_sc0001852.1_g000023 Rmu_sc0001852.1_g000024 Rmu_sc0002434.1_g000001 Rmu_sc0002434.1_g000002 Rmu_sc0003043.1_g000016 Rmu_sc0003153.1_g000005 Rmu_sc0011039.1_g000001 Rmu_sc0031822.1_g000001
rosa_roxburghii Rroxscaffold_2G00095860 Rroxscaffold_2G00095870 Rroxscaffold_2G00109720 Rroxscaffold_2G00139530 Rroxscaffold_2G00139550 Rroxscaffold_2G00139570 Rroxscaffold_2G00139580 Rroxscaffold_2G00139630 Rroxscaffold_2G00139640 Rroxscaffold_4G00328430 Rroxscaffold_4G00328440 Rroxscaffold_4G00328450
rosa_rugosa Rorug02G0114900 Rorug02G0115000 Rorug02G0115000 Rorug02G0115200 Rorug02G0115300 Rorug02G0115400 Rorug02G0115500 Rorug02G0115600 Rorug02G0115600 Rorug02G0115600 Rorug02G0115700 Rorug02G0320300 Rorug02G0431300 Rorug02G0431300 Rorug06G0028200 Rorug07G0241300
rosa_samantha Rh2AG163200 Rh2AG163300 Rh2AG163700 Rh2AG163800 Rh2AG163900 Rh2AG164000 Rh2AG373400 Rh2AG492600 Rh2BG170300 Rh2BG170400 Rh2BG170800 Rh2BG170900 Rh2BG171000 Rh2BG171100 Rh2BG171200 Rh2BG377800 Rh2BG505000 Rh2BG505100 Rh2BG505200 Rh2CG170300 Rh2CG170700 Rh2CG479000 Rh2DG169100 Rh2DG169200 Rh2DG169300 Rh2DG516400 Rh7AG391200 Rh7BG376200 Rh7CG410200 Rh7CG411500 Rh7CG412000 Rh7CG412700 Rh7DG388800
rosa_wichuraiana Rw2G012830 Rw2G012840 Rw2G012870 Rw2G012880 Rw2G012890 Rw2G012900 Rw2G030310 Rw2G040470 Rw2G040480 Rw7G032720 Rw7G032730 Rw7G032750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 614
AciI CCGC 3 cut(s) 49, 140, 203
AclWI GGATC 2 cut(s) 59, 242
AcsI RAATTY 1 cut(s) 331
AcuI CTGAAG 1 cut(s) 604
AfaI GTAC 1 cut(s) 466
AgsI TTSAA 4 cut(s) 94, 111, 211, 358
AluBI AGCT 2 cut(s) 157, 226
AluI AGCT 2 cut(s) 157, 226
Alw21I GWGCWC 1 cut(s) 510
Alw26I GTCTC 4 cut(s) 46, 188, 368, 525
AlwI GGATC 2 cut(s) 59, 242
AoxI GGCC 2 cut(s) 24, 301
ApoI RAATTY 1 cut(s) 331
Asp700I GAANNNNTTC 2 cut(s) 86, 89
AsuHPI GGTGA 5 cut(s) 25, 38, 91, 251, 509
AsuII TTCGAA 1 cut(s) 318
BbsI GAAGAC 2 cut(s) 610, 612
Bbv12I GWGCWC 1 cut(s) 510
BccI CCATC 1 cut(s) 299
BcoDI GTCTC 4 cut(s) 46, 188, 368, 525
BmiI GGNNCC 1 cut(s) 254
BmsI GCATC 1 cut(s) 382
BpiI GAAGAC 2 cut(s) 610, 612
Bpu14I TTCGAA 1 cut(s) 318
BpuEI CTTGAG 1 cut(s) 444
Bsa29I ATCGAT 1 cut(s) 429
BsaI GGTCTC 1 cut(s) 368
Bse118I RCCGGY 1 cut(s) 76
Bse1I ACTGG 1 cut(s) 393
BseCI ATCGAT 1 cut(s) 429
BseGI GGATG 1 cut(s) 397
BseMII CTCAG 2 cut(s) 60, 189
BseNI ACTGG 1 cut(s) 393
BshFI GGCC 2 cut(s) 26, 303
BshVI ATCGAT 1 cut(s) 429
BsiHKAI GWGCWC 1 cut(s) 510
BsiSI CCGG 1 cut(s) 77
BsmAI GTCTC 4 cut(s) 46, 188, 368, 525
BsmBI CGTCTC 1 cut(s) 46
BsnI GGCC 2 cut(s) 26, 303
Bso31I GGTCTC 1 cut(s) 368
Bsp119I TTCGAA 1 cut(s) 318
Bsp1286I GDGCHC 1 cut(s) 510
Bsp143I GATC 5 cut(s) 53, 64, 234, 524, 559
BspACI CCGC 3 cut(s) 49, 140, 203
BspANI GGCC 2 cut(s) 26, 303
BspCNI CTCAG 2 cut(s) 61, 188
BspDI ATCGAT 1 cut(s) 429
BspLI GGNNCC 1 cut(s) 254
BspPI GGATC 2 cut(s) 59, 242
BspT104I TTCGAA 1 cut(s) 318
BspTNI GGTCTC 1 cut(s) 368
BsrFI RCCGGY 1 cut(s) 76
BsrI ACTGG 1 cut(s) 393
BssAI RCCGGY 1 cut(s) 76
BssMI GATC 5 cut(s) 53, 64, 234, 524, 559
Bst6I CTCTTC 2 cut(s) 237, 515
BstBI TTCGAA 1 cut(s) 318
BstC8I GCNNGC 1 cut(s) 370
BstDEI CTNAG 2 cut(s) 69, 175
BstF5I GGATG 1 cut(s) 397
BstKTI GATC 5 cut(s) 56, 67, 237, 527, 562
BstMAI GTCTC 4 cut(s) 46, 188, 368, 525
BstMBI GATC 5 cut(s) 53, 64, 234, 524, 559
BstNSI RCATGY 1 cut(s) 587
BstV2I GAAGAC 2 cut(s) 610, 612
BstX2I RGATCY 1 cut(s) 64
BstYI RGATCY 1 cut(s) 64
Bsu15I ATCGAT 1 cut(s) 429
BsuRI GGCC 2 cut(s) 26, 303
BsuTUI ATCGAT 1 cut(s) 429
BtsCI GGATG 1 cut(s) 397
BtsIMutI CAGTG 1 cut(s) 386
Cac8I GCNNGC 1 cut(s) 370
Cfr10I RCCGGY 1 cut(s) 76
ClaI ATCGAT 1 cut(s) 429
Csp6I GTAC 1 cut(s) 465
CviAII CATG 2 cut(s) 257, 584
CviJI RGCY 6 cut(s) 26, 157, 226, 284, 303, 368
CviKI_1 RGCY 6 cut(s) 26, 157, 226, 284, 303, 368
CviQI GTAC 1 cut(s) 465
DdeI CTNAG 2 cut(s) 69, 175
DpnI GATC 5 cut(s) 55, 66, 236, 526, 561
DpnII GATC 5 cut(s) 53, 64, 234, 524, 559
DrdI GACNNNNNNGTC 1 cut(s) 614
DseDI GACNNNNNNGTC 1 cut(s) 614
Eam1104I CTCTTC 2 cut(s) 237, 515
EarI CTCTTC 2 cut(s) 237, 515
EciI GGCGGA 1 cut(s) 129
Eco31I GGTCTC 1 cut(s) 368
Eco57I CTGAAG 1 cut(s) 604
EcoRI GAATTC 1 cut(s) 331
EcoT22I ATGCAT 1 cut(s) 585
Esp3I CGTCTC 1 cut(s) 46
FaeI CATG 2 cut(s) 260, 587
FaiI YATR 4 cut(s) 258, 402, 425, 585
FalI AAGNNNNNCTT 5 cut(s) 74, 106, 86, 118, 602
FatI CATG 2 cut(s) 256, 583
FauI CCCGC 1 cut(s) 196
FokI GGATG 1 cut(s) 404
HaeIII GGCC 2 cut(s) 26, 303
HapII CCGG 1 cut(s) 77
Hin1II CATG 2 cut(s) 260, 587
HinfI GANTC 8 cut(s) 30, 71, 136, 147, 196, 550, 574, 615
HpaII CCGG 1 cut(s) 77
HphI GGTGA 5 cut(s) 25, 38, 91, 251, 509
Hpy188I TCNGA 1 cut(s) 178
Hpy188III TCNNGA 4 cut(s) 68, 151, 528, 563
Hpy99I CGWCG 1 cut(s) 419
HpyAV CCTTC 4 cut(s) 16, 112, 117, 293
HpyCH4V TGCA 3 cut(s) 278, 395, 583
HpyF3I CTNAG 2 cut(s) 69, 175
Hsp92II CATG 2 cut(s) 260, 587
Kzo9I GATC 5 cut(s) 53, 64, 234, 524, 559
LpnPI CCDG 7 cut(s) 40, 81, 90, 213, 374, 423, 607
LweI GCATC 1 cut(s) 382
MaeIII GTNAC 3 cut(s) 4, 31, 184
MalI GATC 5 cut(s) 55, 66, 236, 526, 561
MboI GATC 5 cut(s) 53, 64, 234, 524, 559
MflI RGATCY 1 cut(s) 64
MhlI GDGCHC 1 cut(s) 510
MluCI AATT 1 cut(s) 331
MlyI GAGTC 4 cut(s) 39, 80, 190, 624
MmeI TCCRAC 2 cut(s) 245, 433
MnlI CCTC 5 cut(s) 46, 172, 253, 274, 289
Mph1103I ATGCAT 1 cut(s) 585
MroXI GAANNNNTTC 2 cut(s) 86, 89
MseI TTAA 3 cut(s) 222, 489, 591
MspI CCGG 1 cut(s) 77
NdeII GATC 5 cut(s) 53, 64, 234, 524, 559
NlaIII CATG 2 cut(s) 260, 587
NlaIV GGNNCC 1 cut(s) 254
NmuCI GTSAC 1 cut(s) 31
NsiI ATGCAT 1 cut(s) 585
NspI RCATGY 1 cut(s) 587
NspV TTCGAA 1 cut(s) 318
PdmI GAANNNNTTC 2 cut(s) 86, 89
PfeI GAWTC 4 cut(s) 136, 147, 550, 574
PleI GAGTC 4 cut(s) 38, 79, 190, 623
PpsI GAGTC 4 cut(s) 38, 79, 190, 623
PspN4I GGNNCC 1 cut(s) 254
PsrI GAACNNNNNNTAC 2 cut(s) 448, 480
PsuI RGATCY 1 cut(s) 64
RsaI GTAC 1 cut(s) 466
RsaNI GTAC 1 cut(s) 465
SaqAI TTAA 3 cut(s) 222, 489, 591
Sau3AI GATC 5 cut(s) 53, 64, 234, 524, 559
SchI GAGTC 4 cut(s) 39, 80, 190, 624
SduI GDGCHC 1 cut(s) 510
SetI ASST 6 cut(s) 38, 109, 159, 183, 228, 466
SfaNI GCATC 1 cut(s) 382
SfuI TTCGAA 1 cut(s) 318
SgrAI CRCCGGYG 1 cut(s) 76
SmlI CTYRAG 1 cut(s) 459
SmoI CTYRAG 1 cut(s) 459
Sse9I AATT 1 cut(s) 331
SsiI CCGC 3 cut(s) 49, 140, 203
TaqI TCGA 5 cut(s) 294, 318, 335, 429, 537
TasI AATT 1 cut(s) 331
TfiI GAWTC 4 cut(s) 136, 147, 550, 574
Tru1I TTAA 3 cut(s) 222, 489, 591
Tru9I TTAA 3 cut(s) 222, 489, 591
TscAI CASTG 1 cut(s) 393
TseFI GTSAC 1 cut(s) 31
Tsp45I GTSAC 1 cut(s) 31
TspDTI ATGAA 4 cut(s) 131, 146, 389, 587
TspGWI ACGGA 3 cut(s) 28, 247, 332
TspRI CASTG 1 cut(s) 393
XapI RAATTY 1 cut(s) 331
XceI RCATGY 1 cut(s) 587
XcmI CCANNNNNNNNNTGG 1 cut(s) 385
XmnI GAANNNNTTC 2 cut(s) 86, 89
Zsp2I ATGCAT 1 cut(s) 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.