B3 domain-containing
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 252 |
| AciI | CCGC | 4 cut(s) | 250, 252, 804, 1570 |
| AclWI | GGATC | 1 cut(s) | 681 |
| AcoI | YGGCCR | 2 cut(s) | 14, 770 |
| AcuI | CTGAAG | 2 cut(s) | 1949, 1979 |
| AfaI | GTAC | 3 cut(s) | 1039, 1911, 2118 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 155, 1640 |
| AflII | CTTAAG | 1 cut(s) | 143 |
| AhlI | ACTAGT | 1 cut(s) | 479 |
| AjnI | CCWGG | 1 cut(s) | 1633 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1105, 1137 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 457, 489 |
| Alw21I | GWGCWC | 2 cut(s) | 1075, 2097 |
| Alw26I | GTCTC | 7 cut(s) | 111, 320, 414, 1018, 1366, 1909, 2070 |
| AlwI | GGATC | 1 cut(s) | 681 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 329 |
| Ama87I | CYCGRG | 1 cut(s) | 1033 |
| AoxI | GGCC | 5 cut(s) | 14, 446, 770, 1787, 1985 |
| ApeKI | GCWGC | 4 cut(s) | 607, 1387, 1916, 2042 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1429 |
| AspS9I | GGNCC | 2 cut(s) | 473, 1567 |
| AsuC2I | CCSGG | 1 cut(s) | 705 |
| AsuHPI | GGTGA | 3 cut(s) | 426, 680, 2008 |
| AsuII | TTCGAA | 3 cut(s) | 294, 846, 1587 |
| AvaI | CYCGRG | 1 cut(s) | 1033 |
| AvaII | GGWCC | 2 cut(s) | 473, 1567 |
| BaeGI | GKGCMC | 1 cut(s) | 903 |
| BalI | TGGCCA | 1 cut(s) | 772 |
| BanII | GRGCYC | 1 cut(s) | 2097 |
| BauI | CACGAG | 1 cut(s) | 1235 |
| Bbv12I | GWGCWC | 2 cut(s) | 1075, 2097 |
| BbvI | GCAGC | 4 cut(s) | 619, 1374, 1903, 2029 |
| BccI | CCATC | 6 cut(s) | 347, 439, 445, 524, 599, 1363 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1822, 1856 |
| BciT130I | CCWGG | 1 cut(s) | 1635 |
| BclI | TGATCA | 1 cut(s) | 1231 |
| BcnI | CCSGG | 1 cut(s) | 705 |
| BcoDI | GTCTC | 7 cut(s) | 111, 320, 414, 1018, 1366, 1909, 2070 |
| BcuI | ACTAGT | 1 cut(s) | 479 |
| BfaI | CTAG | 9 cut(s) | 72, 245, 444, 480, 519, 783, 815, 1041, 1734 |
| BfmI | CTRYAG | 1 cut(s) | 1254 |
| BfrI | CTTAAG | 1 cut(s) | 143 |
| BglII | AGATCT | 1 cut(s) | 1308 |
| BisI | GCNGC | 4 cut(s) | 608, 1388, 1917, 2043 |
| BlsI | GCNGC | 4 cut(s) | 609, 1389, 1918, 2044 |
| BmcAI | AGTACT | 1 cut(s) | 1039 |
| Bme1390I | CCNGG | 2 cut(s) | 705, 1635 |
| Bme18I | GGWCC | 2 cut(s) | 473, 1567 |
| BmeT110I | CYCGRG | 1 cut(s) | 1033 |
| BmgT120I | GGNCC | 2 cut(s) | 473, 1567 |
| BmrFI | CCNGG | 2 cut(s) | 705, 1635 |
| BmsI | GCATC | 3 cut(s) | 84, 358, 728 |
| BoxI | GACNNNNGTC | 1 cut(s) | 252 |
| BpmI | CTGGAG | 1 cut(s) | 1209 |
| Bpu14I | TTCGAA | 3 cut(s) | 294, 846, 1587 |
| BpuEI | CTTGAG | 1 cut(s) | 575 |
| BpuMI | CCSGG | 1 cut(s) | 705 |
| BsaI | GGTCTC | 1 cut(s) | 1018 |
| BsaJI | CCNNGG | 6 cut(s) | 250, 277, 564, 873, 1086, 1634 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 155, 1640 |
| Bse118I | RCCGGY | 1 cut(s) | 1912 |
| Bse3DI | GCAATG | 2 cut(s) | 1126, 1398 |
| BseBI | CCWGG | 1 cut(s) | 1635 |
| BseDI | CCNNGG | 6 cut(s) | 250, 277, 564, 873, 1086, 1634 |
| BseGI | GGATG | 2 cut(s) | 67, 1549 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 155, 1640 |
| BseMI | GCAATG | 2 cut(s) | 1126, 1398 |
| BseMII | CTCAG | 1 cut(s) | 1277 |
| BseRI | GAGGAG | 2 cut(s) | 1251, 1662 |
| BseSI | GKGCMC | 1 cut(s) | 903 |
| BseXI | GCAGC | 4 cut(s) | 619, 1374, 1903, 2029 |
| BseYI | CCCAGC | 1 cut(s) | 2037 |
| Bsh1236I | CGCG | 1 cut(s) | 252 |
| BshFI | GGCC | 5 cut(s) | 16, 448, 772, 1789, 1987 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1075, 2097 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1033 |
| BsiSI | CCGG | 2 cut(s) | 705, 1913 |
| BslFI | GGGAC | 2 cut(s) | 1535, 1829 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 155, 1640 |
| BsmAI | GTCTC | 7 cut(s) | 111, 320, 414, 1018, 1366, 1909, 2070 |
| BsmBI | CGTCTC | 1 cut(s) | 2070 |
| BsmFI | GGGAC | 2 cut(s) | 1535, 1829 |
| BsmI | GAATGC | 1 cut(s) | 1277 |
| BsnI | GGCC | 5 cut(s) | 16, 448, 772, 1789, 1987 |
| Bso31I | GGTCTC | 1 cut(s) | 1018 |
| BsoBI | CYCGRG | 1 cut(s) | 1033 |
| Bsp119I | TTCGAA | 3 cut(s) | 294, 846, 1587 |
| Bsp1286I | GDGCHC | 3 cut(s) | 903, 1075, 2097 |
| Bsp143I | GATC | 6 cut(s) | 673, 1043, 1231, 1308, 1979, 2103 |
| Bsp19I | CCATGG | 1 cut(s) | 1086 |
| BspACI | CCGC | 4 cut(s) | 250, 252, 804, 1570 |
| BspANI | GGCC | 5 cut(s) | 16, 448, 772, 1789, 1987 |
| BspCNI | CTCAG | 1 cut(s) | 1276 |
| BspFNI | CGCG | 1 cut(s) | 252 |
| BspHI | TCATGA | 1 cut(s) | 594 |
| BspPI | GGATC | 1 cut(s) | 681 |
| BspQI | GCTCTTC | 1 cut(s) | 1201 |
| BspT104I | TTCGAA | 3 cut(s) | 294, 846, 1587 |
| BspTI | CTTAAG | 1 cut(s) | 143 |
| BspTNI | GGTCTC | 1 cut(s) | 1018 |
| BsrDI | GCAATG | 2 cut(s) | 1126, 1398 |
| BsrFI | RCCGGY | 1 cut(s) | 1912 |
| BssAI | RCCGGY | 1 cut(s) | 1912 |
| BssECI | CCNNGG | 6 cut(s) | 250, 277, 564, 873, 1086, 1634 |
| BssMI | GATC | 6 cut(s) | 673, 1043, 1231, 1308, 1979, 2103 |
| BssSI | CACGAG | 1 cut(s) | 1235 |
| BssT1I | CCWWGG | 3 cut(s) | 277, 564, 1086 |
| Bst2BI | CACGAG | 1 cut(s) | 1235 |
| Bst2UI | CCWGG | 1 cut(s) | 1635 |
| Bst4CI | ACNGT | 3 cut(s) | 688, 898, 1228 |
| Bst6I | CTCTTC | 1 cut(s) | 1201 |
| BstAFI | CTTAAG | 1 cut(s) | 143 |
| BstBI | TTCGAA | 3 cut(s) | 294, 846, 1587 |
| BstC8I | GCNNGC | 3 cut(s) | 1392, 1396, 1831 |
| BstDEI | CTNAG | 2 cut(s) | 1263, 1864 |
| BstDSI | CCRYGG | 3 cut(s) | 250, 873, 1086 |
| BstF5I | GGATG | 2 cut(s) | 67, 1549 |
| BstFNI | CGCG | 1 cut(s) | 252 |
| BstKTI | GATC | 6 cut(s) | 676, 1046, 1234, 1311, 1982, 2106 |
| BstMAI | GTCTC | 7 cut(s) | 111, 320, 414, 1018, 1366, 1909, 2070 |
| BstMBI | GATC | 6 cut(s) | 673, 1043, 1231, 1308, 1979, 2103 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 145, 1200, 1400, 2048 |
| BstNI | CCWGG | 1 cut(s) | 1635 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 252 |
| BstSCI | CCNGG | 2 cut(s) | 703, 1633 |
| BstSFI | CTRYAG | 1 cut(s) | 1254 |
| BstSLI | GKGCMC | 1 cut(s) | 903 |
| BstUI | CGCG | 1 cut(s) | 252 |
| BstV1I | GCAGC | 4 cut(s) | 619, 1374, 1903, 2029 |
| BstX2I | RGATCY | 2 cut(s) | 673, 1308 |
| BstYI | RGATCY | 2 cut(s) | 673, 1308 |
| BsuRI | GGCC | 5 cut(s) | 16, 448, 772, 1789, 1987 |
| BtgI | CCRYGG | 3 cut(s) | 250, 873, 1086 |
| BtsCI | GGATG | 2 cut(s) | 67, 1549 |
| BtsIMutI | CAGTG | 1 cut(s) | 141 |
| Cac8I | GCNNGC | 3 cut(s) | 1392, 1396, 1831 |
| CaiI | CAGNNNCTG | 1 cut(s) | 329 |
| CciI | TCATGA | 1 cut(s) | 594 |
| Cfr10I | RCCGGY | 1 cut(s) | 1912 |
| Cfr13I | GGNCC | 2 cut(s) | 473, 1567 |
| Cfr42I | CCGCGG | 1 cut(s) | 253 |
| CseI | GACGC | 1 cut(s) | 128 |
| Csp6I | GTAC | 3 cut(s) | 1038, 1910, 2117 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 862, 897 |
| CviAII | CATG | 6 cut(s) | 595, 604, 665, 1087, 1711, 2098 |
| CviQI | GTAC | 3 cut(s) | 1038, 1910, 2117 |
| DdeI | CTNAG | 2 cut(s) | 1263, 1864 |
| DpnI | GATC | 6 cut(s) | 675, 1045, 1233, 1310, 1981, 2105 |
| DpnII | GATC | 6 cut(s) | 673, 1043, 1231, 1308, 1979, 2103 |
| EaeI | YGGCCR | 2 cut(s) | 14, 770 |
| Eam1104I | CTCTTC | 1 cut(s) | 1201 |
| EarI | CTCTTC | 1 cut(s) | 1201 |
| Ecl136II | GAGCTC | 1 cut(s) | 2095 |
| Eco130I | CCWWGG | 3 cut(s) | 277, 564, 1086 |
| Eco147I | AGGCCT | 1 cut(s) | 448 |
| Eco24I | GRGCYC | 1 cut(s) | 2097 |
| Eco31I | GGTCTC | 1 cut(s) | 1018 |
| Eco47I | GGWCC | 2 cut(s) | 473, 1567 |
| Eco53kI | GAGCTC | 1 cut(s) | 2095 |
| Eco57I | CTGAAG | 2 cut(s) | 1949, 1979 |
| Eco88I | CYCGRG | 1 cut(s) | 1033 |
| EcoICRI | GAGCTC | 1 cut(s) | 2095 |
| EcoRI | GAATTC | 3 cut(s) | 283, 835, 2014 |
| EcoRII | CCWGG | 1 cut(s) | 1633 |
| EcoT14I | CCWWGG | 3 cut(s) | 277, 564, 1086 |
| EcoT38I | GRGCYC | 1 cut(s) | 2097 |
| ErhI | CCWWGG | 3 cut(s) | 277, 564, 1086 |
| Esp3I | CGTCTC | 1 cut(s) | 2070 |
| FaeI | CATG | 6 cut(s) | 598, 607, 668, 1090, 1714, 2101 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1581, 1613 |
| FaqI | GGGAC | 2 cut(s) | 1535, 1829 |
| FatI | CATG | 6 cut(s) | 594, 603, 664, 1086, 1710, 2097 |
| FbaI | TGATCA | 1 cut(s) | 1231 |
| Fnu4HI | GCNGC | 4 cut(s) | 608, 1388, 1917, 2043 |
| FokI | GGATG | 2 cut(s) | 74, 1556 |
| FriOI | GRGCYC | 1 cut(s) | 2097 |
| Fsp4HI | GCNGC | 4 cut(s) | 608, 1388, 1917, 2043 |
| FspBI | CTAG | 9 cut(s) | 72, 245, 444, 480, 519, 783, 815, 1041, 1734 |
| GluI | GCNGC | 4 cut(s) | 608, 1388, 1917, 2043 |
| GsaI | CCCAGC | 1 cut(s) | 2041 |
| GsuI | CTGGAG | 1 cut(s) | 1209 |
| HaeIII | GGCC | 5 cut(s) | 16, 448, 772, 1789, 1987 |
| HapII | CCGG | 2 cut(s) | 705, 1913 |
| HgaI | GACGC | 1 cut(s) | 128 |
| Hin1II | CATG | 6 cut(s) | 598, 607, 668, 1090, 1714, 2101 |
| HincII | GTYRAC | 2 cut(s) | 352, 364 |
| HindII | GTYRAC | 2 cut(s) | 352, 364 |
| HindIII | AAGCTT | 1 cut(s) | 1961 |
| HinfI | GANTC | 9 cut(s) | 353, 1082, 1121, 1212, 1697, 1808, 1852, 1948, 1999 |
| HpaII | CCGG | 2 cut(s) | 705, 1913 |
| HphI | GGTGA | 3 cut(s) | 426, 680, 2008 |
| Hpy166II | GTNNAC | 4 cut(s) | 352, 364, 1772, 2128 |
| Hpy8I | GTNNAC | 4 cut(s) | 352, 364, 1772, 2128 |
| HpyAV | CCTTC | 8 cut(s) | 459, 597, 824, 993, 1151, 1418, 1634, 1800 |
| HpyCH4III | ACNGT | 3 cut(s) | 688, 898, 1228 |
| HpyCH4IV | ACGT | 3 cut(s) | 290, 1605, 2079 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 145, 1200, 1400, 2048 |
| HpyF3I | CTNAG | 2 cut(s) | 1263, 1864 |
| HpySE526I | ACGT | 3 cut(s) | 290, 1605, 2079 |
| Hsp92II | CATG | 6 cut(s) | 598, 607, 668, 1090, 1714, 2101 |
| Ksp22I | TGATCA | 1 cut(s) | 1231 |
| KspI | CCGCGG | 1 cut(s) | 253 |
| Kzo9I | GATC | 6 cut(s) | 673, 1043, 1231, 1308, 1979, 2103 |
| LguI | GCTCTTC | 1 cut(s) | 1201 |
| LmnI | GCTCC | 3 cut(s) | 1264, 1618, 1649 |
| Lsp1109I | GCAGC | 4 cut(s) | 619, 1374, 1903, 2029 |
| LweI | GCATC | 3 cut(s) | 84, 358, 728 |
| MaeI | CTAG | 9 cut(s) | 72, 245, 444, 480, 519, 783, 815, 1041, 1734 |
| MaeII | ACGT | 3 cut(s) | 290, 1605, 2079 |
| MaeIII | GTNAC | 7 cut(s) | 178, 254, 806, 1706, 1777, 1867, 1878 |
| MalI | GATC | 6 cut(s) | 675, 1045, 1233, 1310, 1981, 2105 |
| MboI | GATC | 6 cut(s) | 673, 1043, 1231, 1308, 1979, 2103 |
| MboII | GAAGA | 8 cut(s) | 220, 468, 518, 926, 1188, 1437, 1679, 1785 |
| MfeI | CAATTG | 2 cut(s) | 1490, 1746 |
| MflI | RGATCY | 2 cut(s) | 673, 1308 |
| MhlI | GDGCHC | 3 cut(s) | 903, 1075, 2097 |
| MlsI | TGGCCA | 1 cut(s) | 772 |
| MluNI | TGGCCA | 1 cut(s) | 772 |
| MlyI | GAGTC | 1 cut(s) | 347 |
| MmeI | TCCRAC | 1 cut(s) | 500 |
| Mox20I | TGGCCA | 1 cut(s) | 772 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1429 |
| MscI | TGGCCA | 1 cut(s) | 772 |
| Msp20I | TGGCCA | 1 cut(s) | 772 |
| MspA1I | CMGCKG | 2 cut(s) | 252, 1187 |
| MspCI | CTTAAG | 1 cut(s) | 143 |
| MspI | CCGG | 2 cut(s) | 705, 1913 |
| MspR9I | CCNGG | 2 cut(s) | 705, 1635 |
| MunI | CAATTG | 2 cut(s) | 1490, 1746 |
| Mva1269I | GAATGC | 1 cut(s) | 1277 |
| MvaI | CCWGG | 1 cut(s) | 1635 |
| MvnI | CGCG | 1 cut(s) | 252 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 145, 1200, 1400, 2048 |
| NciI | CCSGG | 1 cut(s) | 705 |
| NcoI | CCATGG | 1 cut(s) | 1086 |
| NdeII | GATC | 6 cut(s) | 673, 1043, 1231, 1308, 1979, 2103 |
| NlaIII | CATG | 6 cut(s) | 598, 607, 668, 1090, 1714, 2101 |
| NmuCI | GTSAC | 5 cut(s) | 178, 254, 1706, 1867, 1878 |
| NspV | TTCGAA | 3 cut(s) | 294, 846, 1587 |
| PaeR7I | CTCGAG | 1 cut(s) | 1033 |
| PagI | TCATGA | 1 cut(s) | 594 |
| PceI | AGGCCT | 1 cut(s) | 448 |
| PciSI | GCTCTTC | 1 cut(s) | 1201 |
| PctI | GAATGC | 1 cut(s) | 1277 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1429 |
| PfeI | GAWTC | 8 cut(s) | 1082, 1121, 1212, 1697, 1808, 1852, 1948, 1999 |
| PkrI | GCNGC | 4 cut(s) | 609, 1389, 1918, 2044 |
| PleI | GAGTC | 1 cut(s) | 347 |
| PpsI | GAGTC | 1 cut(s) | 347 |
| PshAI | GACNNNNGTC | 1 cut(s) | 252 |
| Psp124BI | GAGCTC | 1 cut(s) | 2097 |
| Psp6I | CCWGG | 1 cut(s) | 1633 |
| PspFI | CCCAGC | 1 cut(s) | 2037 |
| PspGI | CCWGG | 1 cut(s) | 1633 |
| PspPI | GGNCC | 2 cut(s) | 473, 1567 |
| PspXI | VCTCGAGB | 1 cut(s) | 1033 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 2109, 2141 |
| PstNI | CAGNNNCTG | 1 cut(s) | 329 |
| PsuI | RGATCY | 2 cut(s) | 673, 1308 |
| PvuII | CAGCTG | 1 cut(s) | 1187 |
| RsaI | GTAC | 3 cut(s) | 1039, 1911, 2118 |
| RsaNI | GTAC | 3 cut(s) | 1038, 1910, 2117 |
| SacI | GAGCTC | 1 cut(s) | 2097 |
| SacII | CCGCGG | 1 cut(s) | 253 |
| SapI | GCTCTTC | 1 cut(s) | 1201 |
| SatI | GCNGC | 4 cut(s) | 608, 1388, 1917, 2043 |
| Sau3AI | GATC | 6 cut(s) | 673, 1043, 1231, 1308, 1979, 2103 |
| Sau96I | GGNCC | 2 cut(s) | 473, 1567 |
| ScaI | AGTACT | 1 cut(s) | 1039 |
| SchI | GAGTC | 1 cut(s) | 347 |
| ScrFI | CCNGG | 2 cut(s) | 705, 1635 |
| SduI | GDGCHC | 3 cut(s) | 903, 1075, 2097 |
| SfaNI | GCATC | 3 cut(s) | 84, 358, 728 |
| SfcI | CTRYAG | 1 cut(s) | 1254 |
| Sfr274I | CTCGAG | 1 cut(s) | 1033 |
| Sfr303I | CCGCGG | 1 cut(s) | 253 |
| SfuI | TTCGAA | 3 cut(s) | 294, 846, 1587 |
| SgrBI | CCGCGG | 1 cut(s) | 253 |
| SinI | GGWCC | 2 cut(s) | 473, 1567 |
| SlaI | CTCGAG | 1 cut(s) | 1033 |
| SmlI | CTYRAG | 3 cut(s) | 143, 554, 1033 |
| SmoI | CTYRAG | 3 cut(s) | 143, 554, 1033 |
| SpeI | ACTAGT | 1 cut(s) | 479 |
| SseBI | AGGCCT | 1 cut(s) | 448 |
| SsiI | CCGC | 4 cut(s) | 250, 252, 804, 1570 |
| SspMI | CTAG | 9 cut(s) | 72, 245, 444, 480, 519, 783, 815, 1041, 1734 |
| SstI | GAGCTC | 1 cut(s) | 2097 |
| StuI | AGGCCT | 1 cut(s) | 448 |
| StyD4I | CCNGG | 2 cut(s) | 703, 1633 |
| StyI | CCWWGG | 3 cut(s) | 277, 564, 1086 |
| TaaI | ACNGT | 3 cut(s) | 688, 898, 1228 |
| TaiI | ACGT | 3 cut(s) | 293, 1608, 2082 |
| TaqI | TCGA | 7 cut(s) | 294, 823, 846, 1034, 1418, 1587, 2091 |
| TatI | WGTACW | 2 cut(s) | 1037, 2116 |
| TfiI | GAWTC | 8 cut(s) | 1082, 1121, 1212, 1697, 1808, 1852, 1948, 1999 |
| TscAI | CASTG | 1 cut(s) | 141 |
| TseFI | GTSAC | 5 cut(s) | 178, 254, 1706, 1867, 1878 |
| TseI | GCWGC | 4 cut(s) | 607, 1387, 1916, 2042 |
| Tsp45I | GTSAC | 5 cut(s) | 178, 254, 1706, 1867, 1878 |
| TspGWI | ACGGA | 1 cut(s) | 890 |
| TspRI | CASTG | 1 cut(s) | 141 |
| Vha464I | CTTAAG | 1 cut(s) | 143 |
| VpaK11BI | GGWCC | 2 cut(s) | 473, 1567 |
| XbaI | TCTAGA | 1 cut(s) | 244 |
| XhoI | CTCGAG | 1 cut(s) | 1033 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1429 |
| XspI | CTAG | 9 cut(s) | 72, 245, 444, 480, 519, 783, 815, 1041, 1734 |
| ZrmI | AGTACT | 1 cut(s) | 1039 |