Rroxscaffold_4G00328450

B3 domain-containing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000156
Physical Location & Seq
Forward (+)
61473335 .. 61476291
2957 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00328450.1

Sequence Viewer

Length: 1410 bp
ATGGGTTCTCTTGGCCGGAAAATCTTTTGTGCTATAACTCCCCATTTTTTCAAGATTATTCTGGAGGACACTTCTAGAGAACACAAACTGAAAATTCCAAAGAAATTTATGATAAAATATGGTGAAGATCTCTCAAATTCGGTATGTCTTAAGCTGCCAAGTGGTTCTGAGTGGGAAGTAGAATTGACAAGATGCAATGGTAAGGCTTGGTTTGAAAGGGGTTGGCCAGAGTTCTCTAGATTCTGCTCTCTAAACTACGGCACCTTCCTAGTTTTTCGATATGATGGGAATTCTCACTTTGAAGTTTGTATATTTGATACGAGTGCCACAGAGATTGATTATCCCTTGACAATACCCAAGATTGAAGAAGCTGATGAAGATGATTTATCTATTGACATCTCGGAGGACTTACCTCGCTGCCCAAAAAGTAGGGAGAATTCTTCATCACTCTGTCCTCGGAAGAAAATGAGGACAAGTTTGAGCGGTAAACTAGACATGATTTCTGAAAATGGGAACACTCAACCTGATGACATGGTCGTGTTAGAGAAGCCAATCACAAACAAAGATTCACATTGCTCCAAAGCAGAAATAAAAAGGGAAACTGATTTTGCCACCAAAACTGATGGTGGATGCTTTTCTAGGCCTAAACGATTTCCAAAGCAAACATGCCATGAGAATCTTGGGAGGATGAAGCCATTGACTACAACTGAGAAAGCTATAGCTCTTCAAAGGGCTAATGCTTTCAAATCTGATAAACCTTCTTTCAAGATGGCTATGCAGCCCTCTCATATCCATGGCGGTACTATTAGTTTGCCATATGAATTTGCAAAGAGACATCTTATCAAGCTGCCTGCTGGTATTGCCATCCTTAGAGTATCAGATGGAAGAACTTGGTCTGTGATGTTCAAGTATGATCATAAAAACTCAAAAGCTCGATTATGGAGAAGTGGTTGGTCATCTTTTGTGAGGGACAATAATTTGAAAGTTGGGGATGTGTGTGTCTTTGTCCTTATTGACTGCAATAAACTTTCCTTTGAAGTTTTCTTTTTCCCCACCATAGAAGCTACAAATTGCCGCTTCTCACCAGGCTATGGCAGAGGAGCAATTGTTCAAGTCGAAGAAAAGAAAAGACCCATAGTTGAAGCCGAATCCAACATGAATTGTGAAATTGTTGAGAACAAGATGCTGAAGATTTATGAGCCAGTCACTCAAAAAGCCTTCTTCATCTTTGAGGGCTTCAAGAGTCTTGAAGCTACCAATAAATTTGTCTCAAAGAATCCCTTCTTCTTAGTCTCTCTGCGGTCAATCCAGAGAGTGAAGAAGAATGTGAATGTACCAGCTAGTTTTTTCAAGAGTTTCATGAAGAGGAAGAAACAAACCGTGAAGCTTCAGGTTCAGGATAGATCATAG

Protein Analysis

469

Amino Acids

53.77

Weight (kDa)

9.31

Isoelectric Point (pI)

37.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 17 - 107 7.1e-16 B3 DNA binding domain
B3 PF02362 255 - 347 4.5e-18 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000156)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G49475 AT3G18960 AT3G18960 AT3G18960 AT4G01580
fragaria_vesca FvH4_1g14650 FvH4_1g14650 FvH4_1g14650 FvH4_1g14650 FvH4_1g14660 FvH4_1g14660 FvH4_1g14660 FvH4_1g14660 FvH4_1g14670 FvH4_1g14680 FvH4_1g14680 FvH4_1g14680 FvH4_1g14680 FvH4_1g14680 FvH4_1g14680 FvH4_1g14690 FvH4_1g14700 FvH4_1g29250 FvH4_6g29400 FvH4_6g38681 FvH4_6g38690 FvH4_6g38700 FvH4_6g38700 FvH4_6g38700 FvH4_6g38700 FvH4_6g38700 FvH4_6g38700 FvH4_6g38700 FvH4_7g02010
malus_domestica MD00G1020600.v1.1 MD00G1022000.v1.1 MD00G1022100.v1.1 MD02G1159700.v1.1 MD02G1159800.v1.1 MD02G1159900.v1.1 MD02G1160000.v1.1 MD02G1160300.v1.1 MD15G1274100.v1.1 MD15G1274200.v1.1 MD15G1274400.v1.1 MD15G1302000.v1.1
prunus_persica Prupe.1G579400_v2.0.a1 Prupe.1G579400_v2.0.a1 Prupe.1G579400_v2.0.a1 Prupe.1G579400_v2.0.a1 Prupe.3G083400_v2.0.a1 Prupe.6G229600_v2.0.a1 Prupe.7G039600_v2.0.a1 Prupe.7G044500_v2.0.a1 Prupe.7G044800_v2.0.a1 Prupe.7G143100_v2.0.a1 Prupe.7G143500_v2.0.a1 Prupe.7G143600_v2.0.a1 Prupe.7G143700_v2.0.a1 Prupe.7G143800_v2.0.a1 Prupe.7G143900_v2.0.a1 Prupe.7G143900_v2.0.a1 Prupe.7G143900_v2.0.a1 Prupe.7G143900_v2.0.a1 Prupe.8G072800_v2.0.a1 Prupe.8G072800_v2.0.a1
pyrus_communis pycom02g12620 pycom02g12630 pycom15g23750 pycom15g26490 pycom17g21160
rosa_chinensis RchiOBHm_Chr2g0103421 RchiOBHm_Chr2g0103431 RchiOBHm_Chr2g0103441 RchiOBHm_Chr2g0103491 RchiOBHm_Chr2g0103501 RchiOBHm_Chr2g0103521 RchiOBHm_Chr2g0103541 RchiOBHm_Chr2g0103551 RchiOBHm_Chr2g0103561 RchiOBHm_Chr2g0134821 RchiOBHm_Chr2g0152161 RchiOBHm_Chr2g0152181 RchiOBHm_Chr7g0227261 RchiOBHm_Chr7g0227351 RchiOBHm_Chr7g0227431
rosa_laevigata RLG00000001649 RLG00000001653 RLG00000001659 RLG00000017235 RLG00000017236 RLG00000017240 RLG00000017241 RLG00000017242 RLG00000017243 RLG00000017244 RLG00000017349 RLG00000019424 RLG00000020614 RLG00000020615
rosa_multiflora Rmu_co8247875.1_g000001 Rmu_co8429551.1_g000001 Rmu_sc0000173.1_g000009 Rmu_sc0000718.1_g000004 Rmu_sc0001530.1_g000001 Rmu_sc0001530.1_g000002 Rmu_sc0001530.1_g000004 Rmu_sc0001530.1_g000005 Rmu_sc0001852.1_g000023 Rmu_sc0001852.1_g000024 Rmu_sc0002434.1_g000001 Rmu_sc0002434.1_g000002 Rmu_sc0003043.1_g000016 Rmu_sc0003153.1_g000005 Rmu_sc0011039.1_g000001 Rmu_sc0031822.1_g000001
rosa_roxburghii Rroxscaffold_2G00095860 Rroxscaffold_2G00095870 Rroxscaffold_2G00109720 Rroxscaffold_2G00139530 Rroxscaffold_2G00139550 Rroxscaffold_2G00139570 Rroxscaffold_2G00139580 Rroxscaffold_2G00139630 Rroxscaffold_2G00139640 Rroxscaffold_4G00328430 Rroxscaffold_4G00328440 Rroxscaffold_4G00328450
rosa_rugosa Rorug02G0114900 Rorug02G0115000 Rorug02G0115000 Rorug02G0115200 Rorug02G0115300 Rorug02G0115400 Rorug02G0115500 Rorug02G0115600 Rorug02G0115600 Rorug02G0115600 Rorug02G0115700 Rorug02G0320300 Rorug02G0431300 Rorug02G0431300 Rorug06G0028200 Rorug07G0241300
rosa_samantha Rh2AG163200 Rh2AG163300 Rh2AG163700 Rh2AG163800 Rh2AG163900 Rh2AG164000 Rh2AG373400 Rh2AG492600 Rh2BG170300 Rh2BG170400 Rh2BG170800 Rh2BG170900 Rh2BG171000 Rh2BG171100 Rh2BG171200 Rh2BG377800 Rh2BG505000 Rh2BG505100 Rh2BG505200 Rh2CG170300 Rh2CG170700 Rh2CG479000 Rh2DG169100 Rh2DG169200 Rh2DG169300 Rh2DG516400 Rh7AG391200 Rh7BG376200 Rh7CG410200 Rh7CG411500 Rh7CG412000 Rh7CG412700 Rh7DG388800
rosa_wichuraiana Rw2G012830 Rw2G012840 Rw2G012870 Rw2G012880 Rw2G012890 Rw2G012900 Rw2G030310 Rw2G040470 Rw2G040480 Rw7G032720 Rw7G032730 Rw7G032750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 260
AccB7I CCANNNNNTGG 1 cut(s) 1091
AccBSI CCGCTC 1 cut(s) 483
AciI CCGC 4 cut(s) 483, 798, 1075, 1300
AcoI YGGCCR 2 cut(s) 13, 224
AcsI RAATTY 7 cut(s) 93, 104, 136, 289, 436, 821, 1262
AcuI CTGAAG 2 cut(s) 1208, 1373
AfaI GTAC 2 cut(s) 802, 1335
AfiI CCNNNNNNNGG 1 cut(s) 1091
AflII CTTAAG 1 cut(s) 149
AjnI CCWGG 1 cut(s) 1084
AloI GAACNNNNNNTCC 2 cut(s) 1092, 1124
Alw26I GTCTC 3 cut(s) 826, 1273, 1297
AoxI GGCC 3 cut(s) 13, 224, 641
ApeKI GCWGC 4 cut(s) 154, 417, 778, 847
ApoI RAATTY 7 cut(s) 93, 104, 136, 289, 436, 821, 1262
Asp700I GAANNNNTTC 1 cut(s) 1280
AsuHPI GGTGA 2 cut(s) 134, 1074
BalI TGGCCA 1 cut(s) 226
BanI GGYRCC 1 cut(s) 260
BbvI GCAGC 4 cut(s) 141, 404, 790, 834
BccI CCATC 5 cut(s) 278, 617, 763, 872, 875
BceAI ACGGC 1 cut(s) 274
BciT130I CCWGG 1 cut(s) 1086
BclI TGATCA 1 cut(s) 913
BcoDI GTCTC 3 cut(s) 826, 1273, 1297
BfaI CTAG 6 cut(s) 75, 237, 269, 491, 639, 1341
BfmI CTRYAG 1 cut(s) 717
BfrI CTTAAG 1 cut(s) 149
BglII AGATCT 1 cut(s) 127
BisI GCNGC 5 cut(s) 155, 418, 779, 848, 1075
BlsI GCNGC 5 cut(s) 156, 419, 780, 849, 1076
Bme1390I CCNGG 1 cut(s) 1086
BmiI GGNNCC 1 cut(s) 262
BmrFI CCNGG 1 cut(s) 1086
BmsI GCATC 3 cut(s) 182, 620, 1173
BpmI CTGGAG 1 cut(s) 83
BsaJI CCNNGG 2 cut(s) 455, 793
BsaXI ACNNNNNCTCC 4 cut(s) 934, 964, 1092, 1122
Bsc4I CCNNNNNNNGG 1 cut(s) 1091
Bse1I ACTGG 1 cut(s) 1202
Bse3DI GCAATG 2 cut(s) 202, 571
BseBI CCWGG 1 cut(s) 1086
BseDI CCNNGG 2 cut(s) 455, 793
BseGI GGATG 4 cut(s) 635, 693, 864, 997
BseLI CCNNNNNNNGG 1 cut(s) 1091
BseMI GCAATG 2 cut(s) 202, 571
BseMII CTCAG 2 cut(s) 159, 699
BseNI ACTGG 1 cut(s) 1202
BseRI GAGGAG 1 cut(s) 1113
BseXI GCAGC 4 cut(s) 141, 404, 790, 834
BshFI GGCC 3 cut(s) 15, 226, 643
BshNI GGYRCC 1 cut(s) 260
BsiSI CCGG 1 cut(s) 16
BslFI GGGAC 1 cut(s) 983
BslI CCNNNNNNNGG 1 cut(s) 1091
BsmAI GTCTC 3 cut(s) 826, 1273, 1297
BsmFI GGGAC 1 cut(s) 983
BsnI GGCC 3 cut(s) 15, 226, 643
Bsp143I GATC 3 cut(s) 127, 913, 1403
Bsp19I CCATGG 1 cut(s) 793
BspACI CCGC 4 cut(s) 483, 798, 1075, 1300
BspANI GGCC 3 cut(s) 15, 226, 643
BspCNI CTCAG 2 cut(s) 160, 700
BspHI TCATGA 1 cut(s) 1359
BspLI GGNNCC 1 cut(s) 262
BspQI GCTCTTC 1 cut(s) 729
BspT107I GGYRCC 1 cut(s) 260
BspTI CTTAAG 1 cut(s) 149
BsrBI CCGCTC 1 cut(s) 483
BsrDI GCAATG 2 cut(s) 202, 571
BsrI ACTGG 1 cut(s) 1202
BssECI CCNNGG 2 cut(s) 455, 793
BssMI GATC 3 cut(s) 127, 913, 1403
BssT1I CCWWGG 1 cut(s) 793
Bst2UI CCWGG 1 cut(s) 1086
Bst4CI ACNGT 1 cut(s) 1381
Bst6I CTCTTC 2 cut(s) 729, 1358
BstAFI CTTAAG 1 cut(s) 149
BstC8I GCNNGC 1 cut(s) 852
BstDEI CTNAG 4 cut(s) 168, 708, 869, 1288
BstDSI CCRYGG 1 cut(s) 793
BstF5I GGATG 4 cut(s) 635, 693, 864, 997
BstKTI GATC 3 cut(s) 130, 916, 1406
BstMAI GTCTC 3 cut(s) 826, 1273, 1297
BstMBI GATC 3 cut(s) 127, 913, 1403
BstMWI GCNNNNNNNGC 1 cut(s) 860
BstNI CCWGG 1 cut(s) 1086
BstNSI RCATGY 1 cut(s) 669
BstSCI CCNGG 1 cut(s) 1084
BstSFI CTRYAG 1 cut(s) 717
BstV1I GCAGC 4 cut(s) 141, 404, 790, 834
BstX2I RGATCY 1 cut(s) 127
BstYI RGATCY 1 cut(s) 127
BsuRI GGCC 3 cut(s) 15, 226, 643
BtgI CCRYGG 1 cut(s) 793
BtsCI GGATG 4 cut(s) 635, 693, 864, 997
Cac8I GCNNGC 1 cut(s) 852
CciI TCATGA 1 cut(s) 1359
Csp6I GTAC 2 cut(s) 801, 1334
CspCI CAANNNNNGTGG 2 cut(s) 316, 351
CviAII CATG 7 cut(s) 496, 532, 666, 671, 794, 1156, 1360
CviQI GTAC 2 cut(s) 801, 1334
DdeI CTNAG 4 cut(s) 168, 708, 869, 1288
DpnI GATC 3 cut(s) 129, 915, 1405
DpnII GATC 3 cut(s) 127, 913, 1403
EaeI YGGCCR 2 cut(s) 13, 224
Eam1104I CTCTTC 2 cut(s) 729, 1358
EarI CTCTTC 2 cut(s) 729, 1358
Eco130I CCWWGG 1 cut(s) 793
Eco147I AGGCCT 1 cut(s) 643
Eco57I CTGAAG 2 cut(s) 1208, 1373
EcoRI GAATTC 2 cut(s) 289, 436
EcoRII CCWGG 1 cut(s) 1084
EcoT14I CCWWGG 1 cut(s) 793
ErhI CCWWGG 1 cut(s) 793
FaeI CATG 7 cut(s) 499, 535, 669, 674, 797, 1159, 1363
FalI AAGNNNNNCTT 2 cut(s) 1265, 1297
FaqI GGGAC 1 cut(s) 983
FatI CATG 7 cut(s) 495, 531, 665, 670, 793, 1155, 1359
FauNDI CATATG 1 cut(s) 817
FbaI TGATCA 1 cut(s) 913
Fnu4HI GCNGC 5 cut(s) 155, 418, 779, 848, 1075
FokI GGATG 4 cut(s) 642, 700, 851, 1004
Fsp4HI GCNGC 5 cut(s) 155, 418, 779, 848, 1075
FspBI CTAG 6 cut(s) 75, 237, 269, 491, 639, 1341
GluI GCNGC 5 cut(s) 155, 418, 779, 848, 1075
GsuI CTGGAG 1 cut(s) 83
HaeIII GGCC 3 cut(s) 15, 226, 643
HapII CCGG 1 cut(s) 16
Hin1II CATG 7 cut(s) 499, 535, 669, 674, 797, 1159, 1363
HindIII AAGCTT 1 cut(s) 1385
HinfI GANTC 6 cut(s) 240, 566, 676, 1148, 1243, 1276
HpaII CCGG 1 cut(s) 16
HphI GGTGA 2 cut(s) 134, 1074
Hpy166II GTNNAC 1 cut(s) 488
Hpy188I TCNGA 6 cut(s) 169, 403, 459, 505, 751, 880
Hpy8I GTNNAC 1 cut(s) 488
HpyAV CCTTC 4 cut(s) 274, 768, 1228, 1291
HpyCH4III ACNGT 1 cut(s) 1381
HpyCH4V TGCA 4 cut(s) 195, 778, 827, 1020
HpyF10VI GCNNNNNNNGC 1 cut(s) 860
HpyF3I CTNAG 4 cut(s) 168, 708, 869, 1288
Hsp92II CATG 7 cut(s) 499, 535, 669, 674, 797, 1159, 1363
Ksp22I TGATCA 1 cut(s) 913
Kzo9I GATC 3 cut(s) 127, 913, 1403
LguI GCTCTTC 1 cut(s) 729
LmnI GCTCC 2 cut(s) 581, 1100
Lsp1109I GCAGC 4 cut(s) 141, 404, 790, 834
LweI GCATC 3 cut(s) 182, 620, 1173
MaeI CTAG 6 cut(s) 75, 237, 269, 491, 639, 1341
MaeIII GTNAC 1 cut(s) 1204
MalI GATC 3 cut(s) 129, 915, 1405
MbiI CCGCTC 1 cut(s) 483
MboI GATC 3 cut(s) 127, 913, 1403
MfeI CAATTG 1 cut(s) 1104
MflI RGATCY 1 cut(s) 127
MlsI TGGCCA 1 cut(s) 226
MluNI TGGCCA 1 cut(s) 226
MlyI GAGTC 1 cut(s) 1252
MmeI TCCRAC 1 cut(s) 1176
Mox20I TGGCCA 1 cut(s) 226
MroXI GAANNNNTTC 1 cut(s) 1280
MscI TGGCCA 1 cut(s) 226
MseI TTAA 1 cut(s) 150
MslI CAYNNNNRTG 2 cut(s) 536, 792
Msp20I TGGCCA 1 cut(s) 226
MspCI CTTAAG 1 cut(s) 149
MspI CCGG 1 cut(s) 16
MspR9I CCNGG 1 cut(s) 1086
MunI CAATTG 1 cut(s) 1104
MvaI CCWGG 1 cut(s) 1086
MwoI GCNNNNNNNGC 1 cut(s) 860
NcoI CCATGG 1 cut(s) 793
NdeI CATATG 1 cut(s) 817
NdeII GATC 3 cut(s) 127, 913, 1403
NlaIII CATG 7 cut(s) 499, 535, 669, 674, 797, 1159, 1363
NlaIV GGNNCC 1 cut(s) 262
NmuCI GTSAC 1 cut(s) 1204
NspI RCATGY 1 cut(s) 669
PagI TCATGA 1 cut(s) 1359
PceI AGGCCT 1 cut(s) 643
PciSI GCTCTTC 1 cut(s) 729
PdmI GAANNNNTTC 1 cut(s) 1280
PfeI GAWTC 5 cut(s) 240, 566, 676, 1148, 1276
PflFI GACNNNGTC 1 cut(s) 533
PflMI CCANNNNNTGG 1 cut(s) 1091
PkrI GCNGC 5 cut(s) 156, 419, 780, 849, 1076
PleI GAGTC 1 cut(s) 1251
PpsI GAGTC 1 cut(s) 1251
Psp6I CCWGG 1 cut(s) 1084
PspGI CCWGG 1 cut(s) 1084
PspN4I GGNNCC 1 cut(s) 262
PsuI RGATCY 1 cut(s) 127
PsyI GACNNNGTC 1 cut(s) 533
RsaI GTAC 2 cut(s) 802, 1335
RsaNI GTAC 2 cut(s) 801, 1334
RseI CAYNNNNRTG 2 cut(s) 536, 792
SapI GCTCTTC 1 cut(s) 729
SaqAI TTAA 1 cut(s) 150
SatI GCNGC 5 cut(s) 155, 418, 779, 848, 1075
Sau3AI GATC 3 cut(s) 127, 913, 1403
SchI GAGTC 1 cut(s) 1252
ScrFI CCNGG 1 cut(s) 1086
SfaNI GCATC 3 cut(s) 182, 620, 1173
SfcI CTRYAG 1 cut(s) 717
SmiMI CAYNNNNRTG 2 cut(s) 536, 792
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
SseBI AGGCCT 1 cut(s) 643
SsiI CCGC 4 cut(s) 483, 798, 1075, 1300
SspMI CTAG 6 cut(s) 75, 237, 269, 491, 639, 1341
StuI AGGCCT 1 cut(s) 643
StyD4I CCNGG 1 cut(s) 1084
StyI CCWWGG 1 cut(s) 793
TaaI ACNGT 1 cut(s) 1381
TaqI TCGA 3 cut(s) 277, 934, 1116
TauI GCSGC 1 cut(s) 1077
TfiI GAWTC 5 cut(s) 240, 566, 676, 1148, 1276
Tru1I TTAA 1 cut(s) 150
Tru9I TTAA 1 cut(s) 150
TseFI GTSAC 1 cut(s) 1204
TseI GCWGC 4 cut(s) 154, 417, 778, 847
Tsp45I GTSAC 1 cut(s) 1204
TspDTI ATGAA 8 cut(s) 390, 432, 704, 834, 1172, 1213, 1348, 1376
Tth111I GACNNNGTC 1 cut(s) 533
Van91I CCANNNNNTGG 1 cut(s) 1091
Vha464I CTTAAG 1 cut(s) 149
XapI RAATTY 7 cut(s) 93, 104, 136, 289, 436, 821, 1262
XbaI TCTAGA 2 cut(s) 74, 236
XceI RCATGY 1 cut(s) 669
XcmI CCANNNNNNNNNTGG 1 cut(s) 677
XmnI GAANNNNTTC 1 cut(s) 1280
XspI CTAG 6 cut(s) 75, 237, 269, 491, 639, 1341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.