B3 domain-containing
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 2 cut(s) | 489, 586 |
| Acc36I | ACCTGC | 1 cut(s) | 306 |
| AccIII | TCCGGA | 2 cut(s) | 130, 792 |
| AciI | CCGC | 4 cut(s) | 742, 1236, 1453, 1645 |
| AcoI | YGGCCR | 1 cut(s) | 257 |
| AcsI | RAATTY | 9 cut(s) | 23, 38, 169, 539, 920, 1154, 1256, 1553, 1619 |
| AcuI | CTGAAG | 1 cut(s) | 1457 |
| AfaI | GTAC | 3 cut(s) | 227, 532, 1204 |
| AflIII | ACRYGT | 1 cut(s) | 1228 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 142 |
| AjnI | CCWGG | 2 cut(s) | 814, 838 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 28, 60 |
| AluBI | AGCT | 5 cut(s) | 900, 1013, 1174, 1328, 1340 |
| AluI | AGCT | 5 cut(s) | 900, 1013, 1174, 1328, 1340 |
| Alw26I | GTCTC | 6 cut(s) | 105, 467, 572, 924, 1173, 1285 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1013, 1040 |
| Ama87I | CYCGRG | 1 cut(s) | 1274 |
| Aor13HI | TCCGGA | 2 cut(s) | 130, 792 |
| AoxI | GGCC | 4 cut(s) | 257, 1271, 1454, 1496 |
| ApeKI | GCWGC | 4 cut(s) | 811, 1013, 1239, 1417 |
| ApoI | RAATTY | 9 cut(s) | 23, 38, 169, 539, 920, 1154, 1256, 1553, 1619 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 520, 646, 1195 |
| AspS9I | GGNCC | 3 cut(s) | 588, 1120, 1272 |
| AsuC2I | CCSGG | 1 cut(s) | 20 |
| AsuHPI | GGTGA | 3 cut(s) | 245, 1352, 1595 |
| AvaI | CYCGRG | 1 cut(s) | 1274 |
| AvaII | GGWCC | 2 cut(s) | 588, 1120 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 217, 250 |
| BalI | TGGCCA | 1 cut(s) | 259 |
| BbvI | GCAGC | 4 cut(s) | 823, 1000, 1226, 1404 |
| BccI | CCATC | 5 cut(s) | 1079, 1138, 1475, 1478, 1488 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1073, 1107 |
| BciT130I | CCWGG | 2 cut(s) | 816, 840 |
| BciVI | GTATCC | 1 cut(s) | 384 |
| BcnI | CCSGG | 1 cut(s) | 20 |
| BcoDI | GTCTC | 6 cut(s) | 105, 467, 572, 924, 1173, 1285 |
| BfaI | CTAG | 6 cut(s) | 95, 108, 327, 651, 1200, 1458 |
| BfuAI | ACCTGC | 1 cut(s) | 306 |
| BfuI | GTATCC | 1 cut(s) | 384 |
| BglII | AGATCT | 1 cut(s) | 160 |
| BisI | GCNGC | 6 cut(s) | 812, 1014, 1237, 1240, 1418, 1454 |
| BlsI | GCNGC | 6 cut(s) | 813, 1015, 1238, 1241, 1419, 1455 |
| BmcAI | AGTACT | 1 cut(s) | 1204 |
| Bme1390I | CCNGG | 3 cut(s) | 20, 816, 840 |
| Bme18I | GGWCC | 2 cut(s) | 588, 1120 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 142 |
| BmeT110I | CYCGRG | 1 cut(s) | 1274 |
| BmgT120I | GGNCC | 3 cut(s) | 588, 1120, 1272 |
| BmiI | GGNNCC | 2 cut(s) | 46, 589 |
| BmrFI | CCNGG | 3 cut(s) | 20, 816, 840 |
| BmrI | ACTGGG | 2 cut(s) | 131, 617 |
| BmsI | GCATC | 4 cut(s) | 1063, 1081, 1453, 1576 |
| BmuI | ACTGGG | 2 cut(s) | 131, 617 |
| BpuEI | CTTGAG | 1 cut(s) | 103 |
| BpuMI | CCSGG | 1 cut(s) | 20 |
| BsaI | GGTCTC | 1 cut(s) | 1285 |
| BsaJI | CCNNGG | 2 cut(s) | 1275, 1399 |
| BsaWI | WCCGGW | 3 cut(s) | 33, 130, 792 |
| Bse118I | RCCGGY | 1 cut(s) | 1580 |
| Bse1I | ACTGG | 4 cut(s) | 126, 623, 913, 1298 |
| BseAI | TCCGGA | 2 cut(s) | 130, 792 |
| BseBI | CCWGG | 2 cut(s) | 816, 840 |
| BseDI | CCNNGG | 2 cut(s) | 1275, 1399 |
| BseGI | GGATG | 4 cut(s) | 738, 958, 1294, 1467 |
| BseMII | CTCAG | 4 cut(s) | 562, 774, 917, 1184 |
| BseNI | ACTGG | 4 cut(s) | 126, 623, 913, 1298 |
| BseRI | GAGGAG | 1 cut(s) | 1347 |
| BseXI | GCAGC | 4 cut(s) | 823, 1000, 1226, 1404 |
| BseYI | CCCAGC | 1 cut(s) | 48 |
| BshFI | GGCC | 4 cut(s) | 259, 1273, 1456, 1498 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1274 |
| BsiSI | CCGG | 5 cut(s) | 19, 34, 131, 793, 1581 |
| BsmAI | GTCTC | 6 cut(s) | 105, 467, 572, 924, 1173, 1285 |
| BsmBI | CGTCTC | 1 cut(s) | 1173 |
| BsnI | GGCC | 4 cut(s) | 259, 1273, 1456, 1498 |
| Bso31I | GGTCTC | 1 cut(s) | 1285 |
| BsoBI | CYCGRG | 1 cut(s) | 1274 |
| Bsp13I | TCCGGA | 2 cut(s) | 130, 792 |
| Bsp143I | GATC | 2 cut(s) | 160, 366 |
| BspACI | CCGC | 4 cut(s) | 742, 1236, 1453, 1645 |
| BspANI | GGCC | 4 cut(s) | 259, 1273, 1456, 1498 |
| BspCNI | CTCAG | 4 cut(s) | 561, 775, 918, 1183 |
| BspEI | TCCGGA | 2 cut(s) | 130, 792 |
| BspLI | GGNNCC | 2 cut(s) | 46, 589 |
| BspMI | ACCTGC | 1 cut(s) | 306 |
| BspTNI | GGTCTC | 1 cut(s) | 1285 |
| BsrFI | RCCGGY | 1 cut(s) | 1580 |
| BsrI | ACTGG | 4 cut(s) | 126, 623, 913, 1298 |
| BssAI | RCCGGY | 1 cut(s) | 1580 |
| BssECI | CCNNGG | 2 cut(s) | 1275, 1399 |
| BssMI | GATC | 2 cut(s) | 160, 366 |
| Bst2UI | CCWGG | 2 cut(s) | 816, 840 |
| Bst4CI | ACNGT | 5 cut(s) | 453, 520, 862, 1167, 1426 |
| Bst6I | CTCTTC | 2 cut(s) | 886, 1312 |
| BstDEI | CTNAG | 7 cut(s) | 14, 458, 548, 657, 783, 926, 1170 |
| BstDSI | CCRYGG | 1 cut(s) | 1399 |
| BstF5I | GGATG | 4 cut(s) | 738, 958, 1294, 1467 |
| BstKTI | GATC | 2 cut(s) | 163, 369 |
| BstMAI | GTCTC | 6 cut(s) | 105, 467, 572, 924, 1173, 1285 |
| BstMBI | GATC | 2 cut(s) | 160, 366 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 651, 1586 |
| BstNI | CCWGG | 2 cut(s) | 816, 840 |
| BstNSI | RCATGY | 1 cut(s) | 1232 |
| BstSCI | CCNGG | 3 cut(s) | 18, 814, 838 |
| BstV1I | GCAGC | 4 cut(s) | 823, 1000, 1226, 1404 |
| BstX2I | RGATCY | 1 cut(s) | 160 |
| BstYI | RGATCY | 1 cut(s) | 160 |
| BsuI | GTATCC | 1 cut(s) | 384 |
| BsuRI | GGCC | 4 cut(s) | 259, 1273, 1456, 1498 |
| BtgI | CCRYGG | 1 cut(s) | 1399 |
| BtsCI | GGATG | 4 cut(s) | 738, 958, 1294, 1467 |
| BtsI | GCAGTG | 1 cut(s) | 1532 |
| BtsIMutI | CAGTG | 5 cut(s) | 449, 630, 906, 1038, 1532 |
| BveI | ACCTGC | 1 cut(s) | 306 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1013, 1040 |
| Cfr10I | RCCGGY | 1 cut(s) | 1580 |
| Cfr13I | GGNCC | 3 cut(s) | 588, 1120, 1272 |
| Csp6I | GTAC | 3 cut(s) | 226, 531, 1203 |
| CviAII | CATG | 8 cut(s) | 192, 413, 505, 584, 967, 1222, 1229, 1436 |
| CviQI | GTAC | 3 cut(s) | 226, 531, 1203 |
| DdeI | CTNAG | 7 cut(s) | 14, 458, 548, 657, 783, 926, 1170 |
| DpnI | GATC | 2 cut(s) | 162, 368 |
| DpnII | GATC | 2 cut(s) | 160, 366 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 489, 586 |
| DriI | GACNNNNNGTC | 1 cut(s) | 142 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 489, 586 |
| EaeI | YGGCCR | 1 cut(s) | 257 |
| Eam1104I | CTCTTC | 2 cut(s) | 886, 1312 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 142 |
| EarI | CTCTTC | 2 cut(s) | 886, 1312 |
| EciI | GGCGGA | 1 cut(s) | 731 |
| Eco31I | GGTCTC | 1 cut(s) | 1285 |
| Eco47I | GGWCC | 2 cut(s) | 588, 1120 |
| Eco57I | CTGAAG | 1 cut(s) | 1457 |
| Eco88I | CYCGRG | 1 cut(s) | 1274 |
| EcoRII | CCWGG | 2 cut(s) | 814, 838 |
| Esp3I | CGTCTC | 1 cut(s) | 1173 |
| FaeI | CATG | 8 cut(s) | 195, 416, 508, 587, 970, 1225, 1232, 1439 |
| FatI | CATG | 8 cut(s) | 191, 412, 504, 583, 966, 1221, 1228, 1435 |
| Fnu4HI | GCNGC | 6 cut(s) | 812, 1014, 1237, 1240, 1418, 1454 |
| FokI | GGATG | 4 cut(s) | 725, 965, 1281, 1454 |
| Fsp4HI | GCNGC | 6 cut(s) | 812, 1014, 1237, 1240, 1418, 1454 |
| FspBI | CTAG | 6 cut(s) | 95, 108, 327, 651, 1200, 1458 |
| GluI | GCNGC | 6 cut(s) | 812, 1014, 1237, 1240, 1418, 1454 |
| GsaI | CCCAGC | 1 cut(s) | 52 |
| HaeIII | GGCC | 4 cut(s) | 259, 1273, 1456, 1498 |
| HapII | CCGG | 5 cut(s) | 19, 34, 131, 793, 1581 |
| Hin1II | CATG | 8 cut(s) | 195, 416, 508, 587, 970, 1225, 1232, 1439 |
| HinfI | GANTC | 6 cut(s) | 127, 419, 663, 894, 1103, 1291 |
| HpaII | CCGG | 5 cut(s) | 19, 34, 131, 793, 1581 |
| HphI | GGTGA | 3 cut(s) | 245, 1352, 1595 |
| Hpy188I | TCNGA | 9 cut(s) | 202, 311, 731, 784, 927, 1036, 1316, 1335, 1483 |
| Hpy188III | TCNNGA | 9 cut(s) | 82, 95, 108, 131, 268, 327, 480, 597, 793 |
| HpyAV | CCTTC | 5 cut(s) | 244, 311, 789, 1179, 1481 |
| HpyCH4III | ACNGT | 5 cut(s) | 453, 520, 862, 1167, 1426 |
| HpyCH4V | TGCA | 9 cut(s) | 536, 866, 1016, 1023, 1094, 1160, 1373, 1537, 1561 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 651, 1586 |
| HpyF3I | CTNAG | 7 cut(s) | 14, 458, 548, 657, 783, 926, 1170 |
| Hsp92II | CATG | 8 cut(s) | 195, 416, 508, 587, 970, 1225, 1232, 1439 |
| Kpn2I | TCCGGA | 2 cut(s) | 130, 792 |
| Kzo9I | GATC | 2 cut(s) | 160, 366 |
| LmnI | GCTCC | 2 cut(s) | 1247, 1487 |
| Lsp1109I | GCAGC | 4 cut(s) | 823, 1000, 1226, 1404 |
| LweI | GCATC | 4 cut(s) | 1063, 1081, 1453, 1576 |
| MaeI | CTAG | 6 cut(s) | 95, 108, 327, 651, 1200, 1458 |
| MaeIII | GTNAC | 3 cut(s) | 175, 1003, 1057 |
| MalI | GATC | 2 cut(s) | 162, 368 |
| MboI | GATC | 2 cut(s) | 160, 366 |
| MboII | GAAGA | 4 cut(s) | 410, 612, 903, 1329 |
| MfeI | CAATTG | 1 cut(s) | 360 |
| MflI | RGATCY | 1 cut(s) | 160 |
| MlsI | TGGCCA | 1 cut(s) | 259 |
| MluNI | TGGCCA | 1 cut(s) | 259 |
| MlyI | GAGTC | 1 cut(s) | 903 |
| MmeI | TCCRAC | 1 cut(s) | 893 |
| Mox20I | TGGCCA | 1 cut(s) | 259 |
| MroI | TCCGGA | 2 cut(s) | 130, 792 |
| MroXI | GAANNNNTTC | 3 cut(s) | 520, 646, 1195 |
| MscI | TGGCCA | 1 cut(s) | 259 |
| MseI | TTAA | 4 cut(s) | 117, 381, 1068, 1449 |
| Msp20I | TGGCCA | 1 cut(s) | 259 |
| MspI | CCGG | 5 cut(s) | 19, 34, 131, 793, 1581 |
| MspR9I | CCNGG | 3 cut(s) | 20, 816, 840 |
| MunI | CAATTG | 1 cut(s) | 360 |
| MvaI | CCWGG | 2 cut(s) | 816, 840 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 651, 1586 |
| NciI | CCSGG | 1 cut(s) | 20 |
| NdeII | GATC | 2 cut(s) | 160, 366 |
| NlaIII | CATG | 8 cut(s) | 195, 416, 508, 587, 970, 1225, 1232, 1439 |
| NlaIV | GGNNCC | 2 cut(s) | 46, 589 |
| NmuCI | GTSAC | 2 cut(s) | 1003, 1057 |
| NspI | RCATGY | 1 cut(s) | 1232 |
| PciI | ACATGT | 1 cut(s) | 1228 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 375 |
| PdmI | GAANNNNTTC | 3 cut(s) | 520, 646, 1195 |
| PfeI | GAWTC | 5 cut(s) | 127, 419, 663, 1103, 1291 |
| PkrI | GCNGC | 6 cut(s) | 813, 1015, 1238, 1241, 1419, 1455 |
| PleI | GAGTC | 1 cut(s) | 902 |
| PpsI | GAGTC | 1 cut(s) | 902 |
| PscI | ACATGT | 1 cut(s) | 1228 |
| Psp6I | CCWGG | 2 cut(s) | 814, 838 |
| PspFI | CCCAGC | 1 cut(s) | 48 |
| PspGI | CCWGG | 2 cut(s) | 814, 838 |
| PspN4I | GGNNCC | 2 cut(s) | 46, 589 |
| PspPI | GGNCC | 3 cut(s) | 588, 1120, 1272 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1013, 1040 |
| PsuI | RGATCY | 1 cut(s) | 160 |
| RsaI | GTAC | 3 cut(s) | 227, 532, 1204 |
| RsaNI | GTAC | 3 cut(s) | 226, 531, 1203 |
| SaqAI | TTAA | 4 cut(s) | 117, 381, 1068, 1449 |
| SatI | GCNGC | 6 cut(s) | 812, 1014, 1237, 1240, 1418, 1454 |
| Sau3AI | GATC | 2 cut(s) | 160, 366 |
| Sau96I | GGNCC | 3 cut(s) | 588, 1120, 1272 |
| ScaI | AGTACT | 1 cut(s) | 1204 |
| SchI | GAGTC | 1 cut(s) | 903 |
| ScrFI | CCNGG | 3 cut(s) | 20, 816, 840 |
| SfaNI | GCATC | 4 cut(s) | 1063, 1081, 1453, 1576 |
| SinI | GGWCC | 2 cut(s) | 588, 1120 |
| SmlI | CTYRAG | 1 cut(s) | 82 |
| SmoI | CTYRAG | 1 cut(s) | 82 |
| SsiI | CCGC | 4 cut(s) | 742, 1236, 1453, 1645 |
| SspI | AATATT | 1 cut(s) | 1116 |
| SspMI | CTAG | 6 cut(s) | 95, 108, 327, 651, 1200, 1458 |
| StyD4I | CCNGG | 3 cut(s) | 18, 814, 838 |
| TaaI | ACNGT | 5 cut(s) | 453, 520, 862, 1167, 1426 |
| TaqI | TCGA | 3 cut(s) | 269, 369, 481 |
| TatI | WGTACW | 1 cut(s) | 1202 |
| TauI | GCSGC | 2 cut(s) | 1239, 1456 |
| TfiI | GAWTC | 5 cut(s) | 127, 419, 663, 1103, 1291 |
| Tru1I | TTAA | 4 cut(s) | 117, 381, 1068, 1449 |
| Tru9I | TTAA | 4 cut(s) | 117, 381, 1068, 1449 |
| TscAI | CASTG | 5 cut(s) | 456, 630, 913, 1045, 1539 |
| TseFI | GTSAC | 2 cut(s) | 1003, 1057 |
| TseI | GCWGC | 4 cut(s) | 811, 1013, 1239, 1417 |
| Tsp45I | GTSAC | 2 cut(s) | 1003, 1057 |
| TspGWI | ACGGA | 1 cut(s) | 1388 |
| TspRI | CASTG | 5 cut(s) | 456, 630, 913, 1045, 1539 |
| VpaK11BI | GGWCC | 2 cut(s) | 588, 1120 |
| XapI | RAATTY | 9 cut(s) | 23, 38, 169, 539, 920, 1154, 1256, 1553, 1619 |
| XbaI | TCTAGA | 3 cut(s) | 94, 107, 326 |
| XceI | RCATGY | 1 cut(s) | 1232 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1114 |
| XmnI | GAANNNNTTC | 3 cut(s) | 520, 646, 1195 |
| XspI | CTAG | 6 cut(s) | 95, 108, 327, 651, 1200, 1458 |
| ZrmI | AGTACT | 1 cut(s) | 1204 |