B3 domain-containing
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 904 |
| AciI | CCGC | 2 cut(s) | 4, 268 |
| AcsI | RAATTY | 6 cut(s) | 125, 509, 590, 755, 1046, 1120 |
| AcuI | CTGAAG | 4 cut(s) | 258, 650, 933, 1076 |
| AfaI | GTAC | 1 cut(s) | 1245 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 496, 528 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 777, 809 |
| AluBI | AGCT | 7 cut(s) | 152, 281, 339, 422, 535, 644, 1222 |
| AluI | AGCT | 7 cut(s) | 152, 281, 339, 422, 535, 644, 1222 |
| Alw21I | GWGCWC | 1 cut(s) | 1224 |
| AoxI | GGCC | 4 cut(s) | 5, 926, 1021, 1112 |
| ApeKI | GCWGC | 4 cut(s) | 70, 466, 535, 1169 |
| ApoI | RAATTY | 6 cut(s) | 125, 509, 590, 755, 1046, 1120 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 340, 995 |
| AspS9I | GGNCC | 2 cut(s) | 6, 863 |
| AsuHPI | GGTGA | 1 cut(s) | 686 |
| AvaII | GGWCC | 1 cut(s) | 863 |
| BanII | GRGCYC | 2 cut(s) | 622, 1224 |
| Bbv12I | GWGCWC | 1 cut(s) | 1224 |
| BbvI | GCAGC | 4 cut(s) | 82, 478, 522, 1156 |
| BccI | CCATC | 5 cut(s) | 98, 323, 560, 563, 769 |
| BceAI | ACGGC | 1 cut(s) | 1123 |
| BclI | TGATCA | 1 cut(s) | 601 |
| BfaI | CTAG | 3 cut(s) | 345, 1053, 1131 |
| BfmI | CTRYAG | 1 cut(s) | 819 |
| BfuAI | ACCTGC | 1 cut(s) | 904 |
| BisI | GCNGC | 5 cut(s) | 5, 71, 467, 536, 1170 |
| BlsI | GCNGC | 5 cut(s) | 6, 72, 468, 537, 1171 |
| Bme18I | GGWCC | 1 cut(s) | 863 |
| BmgT120I | GGNCC | 2 cut(s) | 6, 863 |
| BmiI | GGNNCC | 1 cut(s) | 865 |
| BmrI | ACTGGG | 1 cut(s) | 1034 |
| BmsI | GCATC | 2 cut(s) | 714, 1077 |
| BmuI | ACTGGG | 1 cut(s) | 1034 |
| BpuEI | CTTGAG | 1 cut(s) | 598 |
| BsaBI | GATNNNNATC | 1 cut(s) | 957 |
| BsaJI | CCNNGG | 1 cut(s) | 9 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 777, 807 |
| Bse1I | ACTGG | 1 cut(s) | 1040 |
| Bse3DI | GCAATG | 1 cut(s) | 546 |
| Bse8I | GATNNNNATC | 1 cut(s) | 957 |
| BseDI | CCNNGG | 1 cut(s) | 9 |
| BseGI | GGATG | 5 cut(s) | 109, 177, 396, 552, 574 |
| BseJI | GATNNNNATC | 1 cut(s) | 957 |
| BseMI | GCAATG | 1 cut(s) | 546 |
| BseMII | CTCAG | 1 cut(s) | 405 |
| BseNI | ACTGG | 1 cut(s) | 1040 |
| BseRI | GAGGAG | 1 cut(s) | 798 |
| BseXI | GCAGC | 4 cut(s) | 82, 478, 522, 1156 |
| BsgI | GTGCAG | 1 cut(s) | 932 |
| BshFI | GGCC | 4 cut(s) | 7, 928, 1023, 1114 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1224 |
| BslFI | GGGAC | 3 cut(s) | 668, 849, 1022 |
| BsmFI | GGGAC | 3 cut(s) | 668, 849, 1022 |
| BsmI | GAATGC | 1 cut(s) | 845 |
| BsnI | GGCC | 4 cut(s) | 7, 928, 1023, 1114 |
| Bsp1286I | GDGCHC | 2 cut(s) | 622, 1224 |
| Bsp143I | GATC | 1 cut(s) | 601 |
| BspACI | CCGC | 2 cut(s) | 4, 268 |
| BspANI | GGCC | 4 cut(s) | 7, 928, 1023, 1114 |
| BspCNI | CTCAG | 1 cut(s) | 406 |
| BspHI | TCATGA | 1 cut(s) | 376 |
| BspLI | GGNNCC | 1 cut(s) | 865 |
| BspMAI | CTGCAG | 1 cut(s) | 823 |
| BspMI | ACCTGC | 1 cut(s) | 904 |
| BsrDI | GCAATG | 1 cut(s) | 546 |
| BsrI | ACTGG | 1 cut(s) | 1040 |
| BssECI | CCNNGG | 1 cut(s) | 9 |
| BssMI | GATC | 1 cut(s) | 601 |
| Bst4CI | ACNGT | 1 cut(s) | 1084 |
| Bst6I | CTCTTC | 1 cut(s) | 478 |
| BstC8I | GCNNGC | 2 cut(s) | 540, 544 |
| BstDEI | CTNAG | 2 cut(s) | 414, 1273 |
| BstF5I | GGATG | 5 cut(s) | 109, 177, 396, 552, 574 |
| BstKTI | GATC | 1 cut(s) | 604 |
| BstMBI | GATC | 1 cut(s) | 601 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 548, 1175 |
| BstNSI | RCATGY | 1 cut(s) | 1037 |
| BstSFI | CTRYAG | 1 cut(s) | 819 |
| BstV1I | GCAGC | 4 cut(s) | 82, 478, 522, 1156 |
| BsuRI | GGCC | 4 cut(s) | 7, 928, 1023, 1114 |
| BtgZI | GCGATG | 2 cut(s) | 222, 747 |
| BtsCI | GGATG | 5 cut(s) | 109, 177, 396, 552, 574 |
| BtsIMutI | CAGTG | 1 cut(s) | 1089 |
| BveI | ACCTGC | 1 cut(s) | 904 |
| Cac8I | GCNNGC | 2 cut(s) | 540, 544 |
| CciI | TCATGA | 1 cut(s) | 376 |
| Cfr13I | GGNCC | 2 cut(s) | 6, 863 |
| Csp6I | GTAC | 1 cut(s) | 1244 |
| CviAII | CATG | 8 cut(s) | 114, 164, 377, 463, 605, 776, 847, 1034 |
| CviQI | GTAC | 1 cut(s) | 1244 |
| DdeI | CTNAG | 2 cut(s) | 414, 1273 |
| DpnI | GATC | 1 cut(s) | 603 |
| DpnII | GATC | 1 cut(s) | 601 |
| Eam1104I | CTCTTC | 1 cut(s) | 478 |
| EarI | CTCTTC | 1 cut(s) | 478 |
| Ecl136II | GAGCTC | 1 cut(s) | 1222 |
| Eco147I | AGGCCT | 1 cut(s) | 928 |
| Eco24I | GRGCYC | 2 cut(s) | 622, 1224 |
| Eco32I | GATATC | 1 cut(s) | 524 |
| Eco47I | GGWCC | 1 cut(s) | 863 |
| Eco53kI | GAGCTC | 1 cut(s) | 1222 |
| Eco57I | CTGAAG | 4 cut(s) | 258, 650, 933, 1076 |
| EcoICRI | GAGCTC | 1 cut(s) | 1222 |
| EcoRI | GAATTC | 1 cut(s) | 125 |
| EcoRV | GATATC | 1 cut(s) | 524 |
| EcoT38I | GRGCYC | 2 cut(s) | 622, 1224 |
| FaeI | CATG | 8 cut(s) | 117, 167, 380, 466, 608, 779, 850, 1037 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 916, 948, 980, 1012 |
| FaqI | GGGAC | 3 cut(s) | 668, 849, 1022 |
| FatI | CATG | 8 cut(s) | 113, 163, 376, 462, 604, 775, 846, 1033 |
| FbaI | TGATCA | 1 cut(s) | 601 |
| Fnu4HI | GCNGC | 5 cut(s) | 5, 71, 467, 536, 1170 |
| FokI | GGATG | 5 cut(s) | 116, 184, 403, 539, 581 |
| FriOI | GRGCYC | 2 cut(s) | 622, 1224 |
| Fsp4HI | GCNGC | 5 cut(s) | 5, 71, 467, 536, 1170 |
| FspBI | CTAG | 3 cut(s) | 345, 1053, 1131 |
| GluI | GCNGC | 5 cut(s) | 5, 71, 467, 536, 1170 |
| HaeIII | GGCC | 4 cut(s) | 7, 928, 1023, 1114 |
| Hin1II | CATG | 8 cut(s) | 117, 167, 380, 466, 608, 779, 850, 1037 |
| HindIII | AAGCTT | 1 cut(s) | 337 |
| HinfI | GANTC | 4 cut(s) | 156, 357, 624, 991 |
| HphI | GGTGA | 1 cut(s) | 686 |
| Hpy166II | GTNNAC | 1 cut(s) | 1255 |
| Hpy188I | TCNGA | 6 cut(s) | 13, 277, 318, 439, 568, 952 |
| Hpy188III | TCNNGA | 5 cut(s) | 226, 377, 628, 878, 962 |
| Hpy8I | GTNNAC | 1 cut(s) | 1255 |
| HpyCH4III | ACNGT | 1 cut(s) | 1084 |
| HpyCH4IV | ACGT | 1 cut(s) | 176 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 548, 1175 |
| HpyF3I | CTNAG | 2 cut(s) | 414, 1273 |
| HpySE526I | ACGT | 1 cut(s) | 176 |
| Hsp92II | CATG | 8 cut(s) | 117, 167, 380, 466, 608, 779, 850, 1037 |
| Ksp22I | TGATCA | 1 cut(s) | 601 |
| Kzo9I | GATC | 1 cut(s) | 601 |
| Lsp1109I | GCAGC | 4 cut(s) | 82, 478, 522, 1156 |
| LweI | GCATC | 2 cut(s) | 714, 1077 |
| MaeI | CTAG | 3 cut(s) | 345, 1053, 1131 |
| MaeII | ACGT | 1 cut(s) | 176 |
| MaeIII | GTNAC | 2 cut(s) | 80, 916 |
| MalI | GATC | 1 cut(s) | 603 |
| MboI | GATC | 1 cut(s) | 601 |
| MfeI | CAATTG | 1 cut(s) | 401 |
| MhlI | GDGCHC | 2 cut(s) | 622, 1224 |
| MlyI | GAGTC | 1 cut(s) | 618 |
| MmeI | TCCRAC | 1 cut(s) | 341 |
| MnlI | CCTC | 9 cut(s) | 19, 373, 481, 645, 776, 779, 877, 936, 994 |
| MroXI | GAANNNNTTC | 2 cut(s) | 340, 995 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1229 |
| MunI | CAATTG | 1 cut(s) | 401 |
| Mva1269I | GAATGC | 1 cut(s) | 845 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 548, 1175 |
| NdeII | GATC | 1 cut(s) | 601 |
| NlaIII | CATG | 8 cut(s) | 117, 167, 380, 466, 608, 779, 850, 1037 |
| NlaIV | GGNNCC | 1 cut(s) | 865 |
| NspI | RCATGY | 1 cut(s) | 1037 |
| PagI | TCATGA | 1 cut(s) | 376 |
| PceI | AGGCCT | 1 cut(s) | 928 |
| PctI | GAATGC | 1 cut(s) | 845 |
| PdmI | GAANNNNTTC | 2 cut(s) | 340, 995 |
| PfeI | GAWTC | 3 cut(s) | 156, 357, 991 |
| PkrI | GCNGC | 5 cut(s) | 6, 72, 468, 537, 1171 |
| PleI | GAGTC | 1 cut(s) | 618 |
| PpsI | GAGTC | 1 cut(s) | 618 |
| Psp124BI | GAGCTC | 1 cut(s) | 1224 |
| PspN4I | GGNNCC | 1 cut(s) | 865 |
| PspPI | GGNCC | 2 cut(s) | 6, 863 |
| PsrI | GAACNNNNNNTAC | 4 cut(s) | 1126, 1158, 1236, 1268 |
| PstI | CTGCAG | 1 cut(s) | 823 |
| RsaI | GTAC | 1 cut(s) | 1245 |
| RsaNI | GTAC | 1 cut(s) | 1244 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1229 |
| SacI | GAGCTC | 1 cut(s) | 1224 |
| SatI | GCNGC | 5 cut(s) | 5, 71, 467, 536, 1170 |
| Sau3AI | GATC | 1 cut(s) | 601 |
| Sau96I | GGNCC | 2 cut(s) | 6, 863 |
| SchI | GAGTC | 1 cut(s) | 618 |
| SduI | GDGCHC | 2 cut(s) | 622, 1224 |
| SfaNI | GCATC | 2 cut(s) | 714, 1077 |
| SfcI | CTRYAG | 1 cut(s) | 819 |
| SinI | GGWCC | 1 cut(s) | 863 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1229 |
| SmlI | CTYRAG | 1 cut(s) | 613 |
| SmoI | CTYRAG | 1 cut(s) | 613 |
| SseBI | AGGCCT | 1 cut(s) | 928 |
| SsiI | CCGC | 2 cut(s) | 4, 268 |
| SspMI | CTAG | 3 cut(s) | 345, 1053, 1131 |
| SstI | GAGCTC | 1 cut(s) | 1224 |
| StuI | AGGCCT | 1 cut(s) | 928 |
| TaaI | ACNGT | 1 cut(s) | 1084 |
| TaiI | ACGT | 1 cut(s) | 179 |
| TaqI | TCGA | 4 cut(s) | 154, 622, 708, 986 |
| TatI | WGTACW | 1 cut(s) | 1243 |
| TauI | GCSGC | 1 cut(s) | 7 |
| TfiI | GAWTC | 3 cut(s) | 156, 357, 991 |
| TscAI | CASTG | 1 cut(s) | 1089 |
| TseI | GCWGC | 4 cut(s) | 70, 466, 535, 1169 |
| TspDTI | ATGAA | 6 cut(s) | 410, 522, 545, 924, 1051, 1247 |
| TspRI | CASTG | 1 cut(s) | 1089 |
| VpaK11BI | GGWCC | 1 cut(s) | 863 |
| XapI | RAATTY | 6 cut(s) | 125, 509, 590, 755, 1046, 1120 |
| XceI | RCATGY | 1 cut(s) | 1037 |
| XmnI | GAANNNNTTC | 2 cut(s) | 340, 995 |
| XspI | CTAG | 3 cut(s) | 345, 1053, 1131 |