disease resistance
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 392 |
| AccI | GTMKAC | 3 cut(s) | 50, 624, 1657 |
| AciI | CCGC | 5 cut(s) | 418, 957, 1640, 1739, 1775 |
| AclWI | GGATC | 2 cut(s) | 99, 2235 |
| AcoI | YGGCCR | 3 cut(s) | 419, 588, 2166 |
| AcsI | RAATTY | 7 cut(s) | 686, 1886, 1904, 1957, 2082, 2801, 2843 |
| AcuI | CTGAAG | 5 cut(s) | 762, 1608, 1713, 2006, 2324 |
| AfaI | GTAC | 2 cut(s) | 447, 1243 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 568, 597, 893, 1362, 1546, 1803, 2482, 2569, 2787 |
| AflII | CTTAAG | 2 cut(s) | 1207, 1978 |
| AgeI | ACCGGT | 1 cut(s) | 1042 |
| AhdI | GACNNNNNGTC | 2 cut(s) | 1640, 1739 |
| AjnI | CCWGG | 2 cut(s) | 1707, 2262 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 504, 536, 2147, 2179 |
| Alw21I | GWGCWC | 1 cut(s) | 1137 |
| Alw26I | GTCTC | 9 cut(s) | 198, 460, 684, 1382, 1517, 1697, 1812, 2110, 2349 |
| AlwI | GGATC | 2 cut(s) | 99, 2235 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 100, 186 |
| Ama87I | CYCGRG | 3 cut(s) | 67, 1322, 2624 |
| AoxI | GGCC | 6 cut(s) | 360, 419, 588, 986, 2166, 2511 |
| ApaI | GGGCCC | 1 cut(s) | 364 |
| ApeKI | GCWGC | 5 cut(s) | 793, 950, 1610, 2319, 2422 |
| ApoI | RAATTY | 7 cut(s) | 686, 1886, 1904, 1957, 2082, 2801, 2843 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 228, 260 |
| AsiGI | ACCGGT | 1 cut(s) | 1042 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 475, 1062, 2613 |
| AspS9I | GGNCC | 4 cut(s) | 360, 361, 849, 1741 |
| AsuC2I | CCSGG | 2 cut(s) | 262, 888 |
| AsuHPI | GGTGA | 2 cut(s) | 902, 2368 |
| AvaI | CYCGRG | 3 cut(s) | 67, 1322, 2624 |
| AvaII | GGWCC | 2 cut(s) | 849, 1741 |
| AxyI | CCTNAGG | 1 cut(s) | 1802 |
| BaeGI | GKGCMC | 1 cut(s) | 364 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 2777, 2810 |
| BalI | TGGCCA | 1 cut(s) | 2168 |
| BanII | GRGCYC | 1 cut(s) | 364 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 927, 959 |
| BbsI | GAAGAC | 3 cut(s) | 522, 608, 1738 |
| Bbv12I | GWGCWC | 1 cut(s) | 1137 |
| BbvCI | CCTCAGC | 1 cut(s) | 6 |
| BbvI | GCAGC | 5 cut(s) | 780, 937, 1622, 2331, 2409 |
| BccI | CCATC | 6 cut(s) | 636, 2020, 2098, 2343, 2738, 2789 |
| BceAI | ACGGC | 2 cut(s) | 1524, 2383 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 173, 207 |
| BciT130I | CCWGG | 2 cut(s) | 1709, 2264 |
| BciVI | GTATCC | 1 cut(s) | 1005 |
| BcnI | CCSGG | 2 cut(s) | 262, 888 |
| BcoDI | GTCTC | 9 cut(s) | 198, 460, 684, 1382, 1517, 1697, 1812, 2110, 2349 |
| BfaI | CTAG | 8 cut(s) | 81, 564, 1148, 1152, 1529, 2048, 2316, 2531 |
| BfmI | CTRYAG | 3 cut(s) | 951, 2507, 2698 |
| BfrI | CTTAAG | 2 cut(s) | 1207, 1978 |
| BfuI | GTATCC | 1 cut(s) | 1005 |
| BisI | GCNGC | 6 cut(s) | 419, 794, 951, 1611, 2320, 2423 |
| BlsI | GCNGC | 6 cut(s) | 420, 795, 952, 1612, 2321, 2424 |
| Bme1390I | CCNGG | 4 cut(s) | 262, 888, 1709, 2264 |
| Bme18I | GGWCC | 2 cut(s) | 849, 1741 |
| BmeRI | GACNNNNNGTC | 2 cut(s) | 1640, 1739 |
| BmeT110I | CYCGRG | 3 cut(s) | 67, 1322, 2624 |
| BmgT120I | GGNCC | 4 cut(s) | 360, 361, 849, 1741 |
| BmiI | GGNNCC | 5 cut(s) | 362, 851, 2277, 2450, 2683 |
| BmrFI | CCNGG | 4 cut(s) | 262, 888, 1709, 2264 |
| BmrI | ACTGGG | 2 cut(s) | 196, 345 |
| BmuI | ACTGGG | 2 cut(s) | 196, 345 |
| BpiI | GAAGAC | 3 cut(s) | 522, 608, 1738 |
| BplI | GAGNNNNNCTC | 6 cut(s) | 690, 722, 957, 989, 2392, 2424 |
| BpmI | CTGGAG | 2 cut(s) | 2318, 2743 |
| Bpu10I | CCTNAGC | 1 cut(s) | 6 |
| BpuEI | CTTGAG | 4 cut(s) | 666, 1682, 1990, 2753 |
| BpuMI | CCSGG | 2 cut(s) | 262, 888 |
| Bsa29I | ATCGAT | 1 cut(s) | 381 |
| BsaAI | YACGTR | 1 cut(s) | 131 |
| BsaI | GGTCTC | 3 cut(s) | 1382, 1697, 1812 |
| BsaJI | CCNNGG | 4 cut(s) | 1323, 1707, 2490, 2604 |
| BsaWI | WCCGGW | 1 cut(s) | 1042 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 568, 597, 893, 1362, 1546, 1803, 2482, 2569, 2787 |
| Bse118I | RCCGGY | 2 cut(s) | 590, 1042 |
| Bse1I | ACTGG | 4 cut(s) | 191, 340, 1340, 1495 |
| Bse21I | CCTNAGG | 1 cut(s) | 1802 |
| Bse3DI | GCAATG | 1 cut(s) | 2377 |
| BseBI | CCWGG | 2 cut(s) | 1709, 2264 |
| BseCI | ATCGAT | 1 cut(s) | 381 |
| BseDI | CCNNGG | 4 cut(s) | 1323, 1707, 2490, 2604 |
| BseGI | GGATG | 6 cut(s) | 109, 285, 420, 1198, 2272, 2796 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 568, 597, 893, 1362, 1546, 1803, 2482, 2569, 2787 |
| BseMI | GCAATG | 1 cut(s) | 2377 |
| BseMII | CTCAG | 3 cut(s) | 1816, 1853, 2142 |
| BseNI | ACTGG | 4 cut(s) | 191, 340, 1340, 1495 |
| BseRI | GAGGAG | 2 cut(s) | 564, 2841 |
| BseSI | GKGCMC | 1 cut(s) | 364 |
| BseX3I | CGGCCG | 1 cut(s) | 419 |
| BseXI | GCAGC | 5 cut(s) | 780, 937, 1622, 2331, 2409 |
| BseYI | CCCAGC | 1 cut(s) | 1602 |
| Bsh1285I | CGRYCG | 1 cut(s) | 422 |
| BshFI | GGCC | 6 cut(s) | 362, 421, 590, 988, 2168, 2513 |
| BshTI | ACCGGT | 1 cut(s) | 1042 |
| BshVI | ATCGAT | 1 cut(s) | 381 |
| BsiEI | CGRYCG | 1 cut(s) | 422 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1137 |
| BsiHKCI | CYCGRG | 3 cut(s) | 67, 1322, 2624 |
| BsiSI | CCGG | 5 cut(s) | 262, 422, 591, 888, 1043 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 568, 597, 893, 1362, 1546, 1803, 2482, 2569, 2787 |
| BsmAI | GTCTC | 9 cut(s) | 198, 460, 684, 1382, 1517, 1697, 1812, 2110, 2349 |
| BsmBI | CGTCTC | 3 cut(s) | 198, 460, 1517 |
| BsmI | GAATGC | 1 cut(s) | 778 |
| BsnI | GGCC | 6 cut(s) | 362, 421, 590, 988, 2168, 2513 |
| Bso31I | GGTCTC | 3 cut(s) | 1382, 1697, 1812 |
| BsoBI | CYCGRG | 3 cut(s) | 67, 1322, 2624 |
| Bsp120I | GGGCCC | 1 cut(s) | 360 |
| Bsp1286I | GDGCHC | 2 cut(s) | 364, 1137 |
| Bsp143I | GATC | 5 cut(s) | 91, 378, 2227, 2245, 2461 |
| BspACI | CCGC | 5 cut(s) | 418, 957, 1640, 1739, 1775 |
| BspANI | GGCC | 6 cut(s) | 362, 421, 590, 988, 2168, 2513 |
| BspCNI | CTCAG | 3 cut(s) | 1815, 1854, 2143 |
| BspDI | ATCGAT | 1 cut(s) | 381 |
| BspHI | TCATGA | 3 cut(s) | 1762, 2307, 2797 |
| BspLI | GGNNCC | 5 cut(s) | 362, 851, 2277, 2450, 2683 |
| BspMAI | CTGCAG | 1 cut(s) | 955 |
| BspPI | GGATC | 2 cut(s) | 99, 2235 |
| BspTI | CTTAAG | 2 cut(s) | 1207, 1978 |
| BspTNI | GGTCTC | 3 cut(s) | 1382, 1697, 1812 |
| BsrDI | GCAATG | 1 cut(s) | 2377 |
| BsrFI | RCCGGY | 2 cut(s) | 590, 1042 |
| BsrI | ACTGG | 4 cut(s) | 191, 340, 1340, 1495 |
| BssAI | RCCGGY | 2 cut(s) | 590, 1042 |
| BssECI | CCNNGG | 4 cut(s) | 1323, 1707, 2490, 2604 |
| BssMI | GATC | 5 cut(s) | 91, 378, 2227, 2245, 2461 |
| BssNAI | GTATAC | 1 cut(s) | 1658 |
| BssT1I | CCWWGG | 2 cut(s) | 2490, 2604 |
| Bst1107I | GTATAC | 1 cut(s) | 1658 |
| Bst2UI | CCWGG | 2 cut(s) | 1709, 2264 |
| Bst4CI | ACNGT | 8 cut(s) | 610, 1017, 1165, 1183, 1994, 2007, 2671, 2702 |
| Bst6I | CTCTTC | 1 cut(s) | 748 |
| BstAFI | CTTAAG | 2 cut(s) | 1207, 1978 |
| BstBAI | YACGTR | 1 cut(s) | 131 |
| BstC8I | GCNNGC | 6 cut(s) | 307, 955, 1137, 1608, 1677, 2515 |
| BstDEI | CTNAG | 6 cut(s) | 6, 1802, 1862, 2151, 2249, 2564 |
| BstEII | GGTNACC | 2 cut(s) | 2007, 2485 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 1801, 2480 |
| BstF5I | GGATG | 6 cut(s) | 109, 285, 420, 1198, 2272, 2796 |
| BstKTI | GATC | 5 cut(s) | 94, 381, 2230, 2248, 2464 |
| BstMAI | GTCTC | 9 cut(s) | 198, 460, 684, 1382, 1517, 1697, 1812, 2110, 2349 |
| BstMBI | GATC | 5 cut(s) | 91, 378, 2227, 2245, 2461 |
| BstMCI | CGRYCG | 1 cut(s) | 422 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 947, 2536 |
| BstNI | CCWGG | 2 cut(s) | 1709, 2264 |
| BstNSI | RCATGY | 4 cut(s) | 950, 1679, 1796, 2855 |
| BstPI | GGTNACC | 2 cut(s) | 2007, 2485 |
| BstSCI | CCNGG | 4 cut(s) | 260, 886, 1707, 2262 |
| BstSFI | CTRYAG | 3 cut(s) | 951, 2507, 2698 |
| BstSLI | GKGCMC | 1 cut(s) | 364 |
| BstSNI | TACGTA | 1 cut(s) | 131 |
| BstV1I | GCAGC | 5 cut(s) | 780, 937, 1622, 2331, 2409 |
| BstV2I | GAAGAC | 3 cut(s) | 522, 608, 1738 |
| BstX2I | RGATCY | 1 cut(s) | 2227 |
| BstYI | RGATCY | 1 cut(s) | 2227 |
| BstZ17I | GTATAC | 1 cut(s) | 1658 |
| BstZI | CGGCCG | 1 cut(s) | 419 |
| Bsu15I | ATCGAT | 1 cut(s) | 381 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1802 |
| BsuI | GTATCC | 1 cut(s) | 1005 |
| BsuRI | GGCC | 6 cut(s) | 362, 421, 590, 988, 2168, 2513 |
| BsuTUI | ATCGAT | 1 cut(s) | 381 |
| BtsCI | GGATG | 6 cut(s) | 109, 285, 420, 1198, 2272, 2796 |
| BtsIMutI | CAGTG | 3 cut(s) | 1347, 1999, 2676 |
| Cac8I | GCNNGC | 6 cut(s) | 307, 955, 1137, 1608, 1677, 2515 |
| CaiI | CAGNNNCTG | 2 cut(s) | 100, 186 |
| CciI | TCATGA | 3 cut(s) | 1762, 2307, 2797 |
| Cfr10I | RCCGGY | 2 cut(s) | 590, 1042 |
| Cfr13I | GGNCC | 4 cut(s) | 360, 361, 849, 1741 |
| ClaI | ATCGAT | 1 cut(s) | 381 |
| CseI | GACGC | 2 cut(s) | 215, 298 |
| Csp6I | GTAC | 2 cut(s) | 446, 1242 |
| CspAI | ACCGGT | 1 cut(s) | 1042 |
| CviQI | GTAC | 2 cut(s) | 446, 1242 |
| DdeI | CTNAG | 6 cut(s) | 6, 1802, 1862, 2151, 2249, 2564 |
| DpnI | GATC | 5 cut(s) | 93, 380, 2229, 2247, 2463 |
| DpnII | GATC | 5 cut(s) | 91, 378, 2227, 2245, 2461 |
| DraI | TTTAAA | 1 cut(s) | 1902 |
| DriI | GACNNNNNGTC | 2 cut(s) | 1640, 1739 |
| EaeI | YGGCCR | 3 cut(s) | 419, 588, 2166 |
| EagI | CGGCCG | 1 cut(s) | 419 |
| Eam1104I | CTCTTC | 1 cut(s) | 748 |
| Eam1105I | GACNNNNNGTC | 2 cut(s) | 1640, 1739 |
| EarI | CTCTTC | 1 cut(s) | 748 |
| EciI | GGCGGA | 2 cut(s) | 972, 1764 |
| EclXI | CGGCCG | 1 cut(s) | 419 |
| Eco105I | TACGTA | 1 cut(s) | 131 |
| Eco130I | CCWWGG | 2 cut(s) | 2490, 2604 |
| Eco147I | AGGCCT | 1 cut(s) | 2513 |
| Eco24I | GRGCYC | 1 cut(s) | 364 |
| Eco31I | GGTCTC | 3 cut(s) | 1382, 1697, 1812 |
| Eco32I | GATATC | 4 cut(s) | 229, 647, 1951, 2313 |
| Eco47I | GGWCC | 2 cut(s) | 849, 1741 |
| Eco52I | CGGCCG | 1 cut(s) | 419 |
| Eco57I | CTGAAG | 5 cut(s) | 762, 1608, 1713, 2006, 2324 |
| Eco81I | CCTNAGG | 1 cut(s) | 1802 |
| Eco88I | CYCGRG | 3 cut(s) | 67, 1322, 2624 |
| Eco91I | GGTNACC | 2 cut(s) | 2007, 2485 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 1801, 2480 |
| EcoO109I | RGGNCCY | 1 cut(s) | 360 |
| EcoO65I | GGTNACC | 2 cut(s) | 2007, 2485 |
| EcoRII | CCWGG | 2 cut(s) | 1707, 2262 |
| EcoRV | GATATC | 4 cut(s) | 229, 647, 1951, 2313 |
| EcoT14I | CCWWGG | 2 cut(s) | 2490, 2604 |
| EcoT22I | ATGCAT | 3 cut(s) | 862, 1426, 2857 |
| EcoT38I | GRGCYC | 1 cut(s) | 364 |
| ErhI | CCWWGG | 2 cut(s) | 2490, 2604 |
| Esp3I | CGTCTC | 3 cut(s) | 198, 460, 1517 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 2474, 2506, 2597, 2629 |
| FauNDI | CATATG | 1 cut(s) | 1429 |
| FblI | GTMKAC | 3 cut(s) | 50, 624, 1657 |
| Fnu4HI | GCNGC | 6 cut(s) | 419, 794, 951, 1611, 2320, 2423 |
| FokI | GGATG | 6 cut(s) | 116, 292, 427, 1205, 2279, 2803 |
| FriOI | GRGCYC | 1 cut(s) | 364 |
| Fsp4HI | GCNGC | 6 cut(s) | 419, 794, 951, 1611, 2320, 2423 |
| FspBI | CTAG | 8 cut(s) | 81, 564, 1148, 1152, 1529, 2048, 2316, 2531 |
| GluI | GCNGC | 6 cut(s) | 419, 794, 951, 1611, 2320, 2423 |
| GsaI | CCCAGC | 1 cut(s) | 1606 |
| GsuI | CTGGAG | 2 cut(s) | 2318, 2743 |
| HaeIII | GGCC | 6 cut(s) | 362, 421, 590, 988, 2168, 2513 |
| HapII | CCGG | 5 cut(s) | 262, 422, 591, 888, 1043 |
| HgaI | GACGC | 2 cut(s) | 215, 298 |
| HincII | GTYRAC | 3 cut(s) | 172, 1179, 2778 |
| HindII | GTYRAC | 3 cut(s) | 172, 1179, 2778 |
| HpaI | GTTAAC | 2 cut(s) | 1179, 2778 |
| HpaII | CCGG | 5 cut(s) | 262, 422, 591, 888, 1043 |
| HphI | GGTGA | 2 cut(s) | 902, 2368 |
| Hpy99I | CGWCG | 2 cut(s) | 314, 2462 |
| HpyCH4III | ACNGT | 8 cut(s) | 610, 1017, 1165, 1183, 1994, 2007, 2671, 2702 |
| HpyCH4IV | ACGT | 5 cut(s) | 130, 168, 519, 1555, 2056 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 947, 2536 |
| HpyF3I | CTNAG | 6 cut(s) | 6, 1802, 1862, 2151, 2249, 2564 |
| HpySE526I | ACGT | 5 cut(s) | 130, 168, 519, 1555, 2056 |
| KspAI | GTTAAC | 2 cut(s) | 1179, 2778 |
| Kzo9I | GATC | 5 cut(s) | 91, 378, 2227, 2245, 2461 |
| LmnI | GCTCC | 4 cut(s) | 1390, 1777, 2681, 2828 |
| Lsp1109I | GCAGC | 5 cut(s) | 780, 937, 1622, 2331, 2409 |
| MaeI | CTAG | 8 cut(s) | 81, 564, 1148, 1152, 1529, 2048, 2316, 2531 |
| MaeII | ACGT | 5 cut(s) | 130, 168, 519, 1555, 2056 |
| MaeIII | GTNAC | 9 cut(s) | 1265, 1433, 1857, 1866, 2007, 2146, 2356, 2485, 2860 |
| MalI | GATC | 5 cut(s) | 93, 380, 2229, 2247, 2463 |
| MboI | GATC | 5 cut(s) | 91, 378, 2227, 2245, 2461 |
| MfeI | CAATTG | 1 cut(s) | 2426 |
| MflI | RGATCY | 1 cut(s) | 2227 |
| MhlI | GDGCHC | 2 cut(s) | 364, 1137 |
| MlsI | TGGCCA | 1 cut(s) | 2168 |
| MluNI | TGGCCA | 1 cut(s) | 2168 |
| MlyI | GAGTC | 3 cut(s) | 55, 78, 2688 |
| MmeI | TCCRAC | 5 cut(s) | 9, 257, 392, 526, 1804 |
| Mox20I | TGGCCA | 1 cut(s) | 2168 |
| Mph1103I | ATGCAT | 3 cut(s) | 862, 1426, 2857 |
| MroXI | GAANNNNTTC | 3 cut(s) | 475, 1062, 2613 |
| MscI | TGGCCA | 1 cut(s) | 2168 |
| Msp20I | TGGCCA | 1 cut(s) | 2168 |
| MspA1I | CMGCKG | 1 cut(s) | 1606 |
| MspCI | CTTAAG | 2 cut(s) | 1207, 1978 |
| MspI | CCGG | 5 cut(s) | 262, 422, 591, 888, 1043 |
| MspR9I | CCNGG | 4 cut(s) | 262, 888, 1709, 2264 |
| MunI | CAATTG | 1 cut(s) | 2426 |
| Mva1269I | GAATGC | 1 cut(s) | 778 |
| MvaI | CCWGG | 2 cut(s) | 1709, 2264 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 947, 2536 |
| NciI | CCSGG | 2 cut(s) | 262, 888 |
| NdeI | CATATG | 1 cut(s) | 1429 |
| NdeII | GATC | 5 cut(s) | 91, 378, 2227, 2245, 2461 |
| NlaIV | GGNNCC | 5 cut(s) | 362, 851, 2277, 2450, 2683 |
| NmeAIII | GCCGAG | 3 cut(s) | 814, 964, 1968 |
| NmuCI | GTSAC | 2 cut(s) | 1866, 2356 |
| NsiI | ATGCAT | 3 cut(s) | 862, 1426, 2857 |
| NspI | RCATGY | 4 cut(s) | 950, 1679, 1796, 2855 |
| PaeI | GCATGC | 1 cut(s) | 1679 |
| PaeR7I | CTCGAG | 2 cut(s) | 67, 2624 |
| PagI | TCATGA | 3 cut(s) | 1762, 2307, 2797 |
| PceI | AGGCCT | 1 cut(s) | 2513 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 180 |
| PctI | GAATGC | 1 cut(s) | 778 |
| PdmI | GAANNNNTTC | 3 cut(s) | 475, 1062, 2613 |
| PfeI | GAWTC | 9 cut(s) | 32, 97, 236, 431, 866, 1058, 1400, 2137, 2439 |
| PflFI | GACNNNGTC | 1 cut(s) | 176 |
| PfoI | TCCNGGA | 2 cut(s) | 260, 2262 |
| PinAI | ACCGGT | 1 cut(s) | 1042 |
| PkrI | GCNGC | 6 cut(s) | 420, 795, 952, 1612, 2321, 2424 |
| PleI | GAGTC | 3 cut(s) | 55, 78, 2688 |
| PpsI | GAGTC | 3 cut(s) | 55, 78, 2688 |
| Ppu21I | YACGTR | 1 cut(s) | 131 |
| PsiI | TTATAA | 1 cut(s) | 392 |
| Psp6I | CCWGG | 2 cut(s) | 1707, 2262 |
| PspEI | GGTNACC | 2 cut(s) | 2007, 2485 |
| PspFI | CCCAGC | 1 cut(s) | 1602 |
| PspGI | CCWGG | 2 cut(s) | 1707, 2262 |
| PspN4I | GGNNCC | 5 cut(s) | 362, 851, 2277, 2450, 2683 |
| PspOMI | GGGCCC | 1 cut(s) | 360 |
| PspPI | GGNCC | 4 cut(s) | 360, 361, 849, 1741 |
| PspXI | VCTCGAGB | 1 cut(s) | 2624 |
| PstI | CTGCAG | 1 cut(s) | 955 |
| PstNI | CAGNNNCTG | 2 cut(s) | 100, 186 |
| PsuI | RGATCY | 1 cut(s) | 2227 |
| PsyI | GACNNNGTC | 1 cut(s) | 176 |
| PvuII | CAGCTG | 1 cut(s) | 1606 |
| RsaI | GTAC | 2 cut(s) | 447, 1243 |
| RsaNI | GTAC | 2 cut(s) | 446, 1242 |
| SatI | GCNGC | 6 cut(s) | 419, 794, 951, 1611, 2320, 2423 |
| Sau3AI | GATC | 5 cut(s) | 91, 378, 2227, 2245, 2461 |
| Sau96I | GGNCC | 4 cut(s) | 360, 361, 849, 1741 |
| SchI | GAGTC | 3 cut(s) | 55, 78, 2688 |
| ScrFI | CCNGG | 4 cut(s) | 262, 888, 1709, 2264 |
| SduI | GDGCHC | 2 cut(s) | 364, 1137 |
| SfcI | CTRYAG | 3 cut(s) | 951, 2507, 2698 |
| Sfr274I | CTCGAG | 2 cut(s) | 67, 2624 |
| SinI | GGWCC | 2 cut(s) | 849, 1741 |
| SlaI | CTCGAG | 2 cut(s) | 67, 2624 |
| SmlI | CTYRAG | 8 cut(s) | 67, 681, 1207, 1661, 1969, 1978, 2624, 2768 |
| SmoI | CTYRAG | 8 cut(s) | 67, 681, 1207, 1661, 1969, 1978, 2624, 2768 |
| SnaBI | TACGTA | 1 cut(s) | 131 |
| SphI | GCATGC | 1 cut(s) | 1679 |
| SseBI | AGGCCT | 1 cut(s) | 2513 |
| SsiI | CCGC | 5 cut(s) | 418, 957, 1640, 1739, 1775 |
| SspI | AATATT | 2 cut(s) | 912, 1307 |
| SspMI | CTAG | 8 cut(s) | 81, 564, 1148, 1152, 1529, 2048, 2316, 2531 |
| StuI | AGGCCT | 1 cut(s) | 2513 |
| StyD4I | CCNGG | 4 cut(s) | 260, 886, 1707, 2262 |
| StyI | CCWWGG | 2 cut(s) | 2490, 2604 |
| TaaI | ACNGT | 8 cut(s) | 610, 1017, 1165, 1183, 1994, 2007, 2671, 2702 |
| TaiI | ACGT | 5 cut(s) | 133, 171, 522, 1558, 2059 |
| TauI | GCSGC | 1 cut(s) | 421 |
| TfiI | GAWTC | 9 cut(s) | 32, 97, 236, 431, 866, 1058, 1400, 2137, 2439 |
| TscAI | CASTG | 3 cut(s) | 1347, 1999, 2676 |
| TseFI | GTSAC | 2 cut(s) | 1866, 2356 |
| TseI | GCWGC | 5 cut(s) | 793, 950, 1610, 2319, 2422 |
| Tsp45I | GTSAC | 2 cut(s) | 1866, 2356 |
| TspDTI | ATGAA | 7 cut(s) | 444, 785, 1103, 1239, 1251, 1931, 2814 |
| TspGWI | ACGGA | 5 cut(s) | 68, 104, 285, 1767, 2803 |
| TspRI | CASTG | 3 cut(s) | 1347, 1999, 2676 |
| Tth111I | GACNNNGTC | 1 cut(s) | 176 |
| Vha464I | CTTAAG | 2 cut(s) | 1207, 1978 |
| VpaK11BI | GGWCC | 2 cut(s) | 849, 1741 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 1801, 2480 |
| XapI | RAATTY | 7 cut(s) | 686, 1886, 1904, 1957, 2082, 2801, 2843 |
| XceI | RCATGY | 4 cut(s) | 950, 1679, 1796, 2855 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 2094 |
| XhoI | CTCGAG | 2 cut(s) | 67, 2624 |
| XmiI | GTMKAC | 3 cut(s) | 50, 624, 1657 |
| XmnI | GAANNNNTTC | 3 cut(s) | 475, 1062, 2613 |
| XspI | CTAG | 8 cut(s) | 81, 564, 1148, 1152, 1529, 2048, 2316, 2531 |
| Zsp2I | ATGCAT | 3 cut(s) | 862, 1426, 2857 |