MD12G1256300.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000069
Physical Location & Seq
Reverse (-)
32403469 .. 32409384
5916 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1256300.v1.1.491

Sequence Viewer

Length: 2802 bp
ATGGCAGAGTTTGTGGTATCATTTGTTGTTGAGAGACTTGGGGACCTGCTAATCGAGAAAGCCACCTTGTTACATGGGGTCAAGCAAAACGTTGAGCAGATCGGACTCGAACTAAGGCGGATGCAATGCTTCTTGAAAGATGCTGATAAGAGGCAAGACGAAGATGATAGCCTTCGAAATTGGGTTTCCGAGATCAGAGAAGTTGCTTACGATGCCGAGGATGTCATTGGGACTTTTACCATCAAAATTGCAACACCAATTTCCAATCCACTAAAAAGGTACGCTTGTTTTTTCGACCGAGCCTCAAACCTTAATCAAGTTGGGTCTGAGATTGAGGCCATCAAAGCTAGAATCTCTGACCTTACTAGAAGTACACAAACTTACGGCTTGAGTGTTGTAAGAGATCACCAGGGCTCGTCGAGTATCGCGTTTGAGAAGCAAAGACAATTGAGGTGGTCTTATTCCCATGTTATTGATGACCATATCGTTGGTTTACAAGGAAATATAAACGAACTAGTGGTGGAGCTCATGAATGAAGAAAAACACGGTCGAGTTGTGTCCATTTGTGGGATGGGTGGTTTGGGAAAGACCACTCTTGCCAAGGAAGTTTACCGCAATGATCGTGTTAGGCGTTATTTTGAGGGTTCTGCGTGGGCATATATATCTCAACAATGCAAACCAAGGGATGTTTGGGAAGGTATTCTGATTAAACTAACCTCTCCGTCGAAAGAGGAGAGGGATCATATTTTGAAATTGAGAGACGAAGAGCTTGCAAAGAAGCTTTATCAAGTTCAAATGGAGAAGAAATATTTGATGGTTCTTGATGACATTTGGACGATTGAGGCCTGGAAAATTCTAAGTCCTGCCTTCCCATCGTCGGGAAAAGGGTGCAGCAGAATATTGCTTACAACTCGTAAAAAAGATGTAGCTTCCTTTGTAGACCGAAGCGGTCTTCATGAGCCGAGGAATTTAACTGAAGAAGAGGGTTGGGAGTTGCTTCAGAAGAAGGCAGTTCCAAGAAATGGTAATCCAGATTTTATCAGGAGCAAAGATAAGGAGCAGTTAGGGAGGGAAATTGTCAAAAAGTGTGCTGGCTTGCCTTTGGCCATTGTTGTGCTTGGCGGACTTTTGGCTACTAAAGAAACAGTGCACGAGTGGGATATCGTCCACAGAGATATTCTTTCGTATCTAAAGCGAGCCAAAGGGGACGAGCAACACTCTACGGTACCTGAGGTTTTGGCTTTGAGCTATCATGATTTGCCTTTTCAATTAAAACCCTGTTTCCTTTATTTGAGTCACTTTCCGGAAGATTTTGAAATTCCGCGGAGAAAACTGGTTCAATTATGGATTGCAGAAGGCATTGTATCGCCACATCATGAAGCAGAGGGAGATGAAACAATAGAGGATGTTGCAGAACGCTACTTGGGTTACTTGATTAATAGGTGCATGGTTCAAGTTGGAACTCTTGGATCAACCGGAAAAATTAAAACTTGCCGCCTCCATGATCTTATGCGTGACTTGTGTTTGTCGAAAGCAAAGCAGGAAAACTTTCTCCAGATTATTCATTATCCGGATGAAAATATGGTGGTGAATTCTTCTTCTTCTCGCATGCTTTCTGAAACAACATCAACTGGTAAAACTCGCAGACTTGCTGTCTTCTTACCTTCACAAGTCGATAATCTTATCCCATCCAAATACAAAGAAGATTCCCACCTCAGTCTCAGGTCCCTTATATTTTTCCATGCAAGCAAGTGTAGATTGGTAAATTGGCTGCTTACAAGAACAATTTTTGAGTTCAAAATGCTCAAAGTTCTCGATCTTGAAGGTGTCAAGGGACCGTATGAAAAGTTACCTAAAGATATCGGGGACTTGGTTCAGTTGCAGTTTCTAAGTTTGAAGAAAACACATATACAAGCTTTGCCATCATCCATCGGCAATTTGATCCACTTGAAAACTTTAAATTTGCAAACCATAAGTAAATTGAGTTGGGATCTGACAGTGCAAATTCCGAATGTGATCTGGAAGATGGAAAGGCTAAGGCATTTATATCTTCCCAAGTGGTGTGGCAATGCAGTTGATAAGCTGCAACTGGGAAATCTCATCAACTTGCAGACACTTGTAAACTTTCCTGCAAACAAATGTGACGTAGAAGATCTTCGCAAGTTGACCAATCTCAGAAAATTGGTGTTGAATGATCCAAAACATTTCAAAAGTTTGGTGAAAATTTTCAGTCCCCAAAGCAGAACGTTAAGCTGCCTCGAGTCCCTGTCCTTGACAAGTGAGACACTTTCGTTCCCTGATGATGGTGTTGATGTTAGACAACTAATGTTAAGTTGTCGCCGTCTACAAAAATTGCACGTGGAAGGGCGGATAGAGAAGCTGCCGGAATACCATCAGTTCCCTCCAAACCTTGCCAAGTTAACATTGTGGGGCTCTAACCTTGAGGAAGATCCAATGCCAACACTGGAAAGACTGCCTAACTTGAGAATTTTAAGCGGATGGCAAATGTTCGCTGGGAAGAAAATGGTCTGCTCTAACCAAGGTTTCCCAAAACTCAAGTCTCTACTCCTCCGAGGGTTTTCTAATTTAGAGGATTGGACGATGGAAGAAGGAGCCATGCCTAGTCTTTGTCGTTTGGAAATATCGAGTTGCATCAAACTGAAGATGATTCCGGATTCTCTGAGGTTTGTGAAAACACTTCAGGAATTGGAGATATATGGATGCCTATTCAAGGTCAACATGGGAAGTGAAGGAGAGGATTTTTACAAAGTGCAACATGTACCGTCCATTGATCAAGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

934

Amino Acids

106.86

Weight (kDa)

8.48

Isoelectric Point (pI)

45.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 5 - 85 4.6e-27 Rx N-terminal domain
NB-ARC PF00931 167 - 341 1e-40 NB-ARC domain
WHD_DRP PF23559 434 - 508 1.8e-19 Disease resistance protein Winged helix domain
LRR_14 PF23598 574 - 890 6.8e-40 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000069)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10920 AT1G10920 AT1G10920 AT1G10920 AT1G10920 AT1G50180 AT1G53350 AT1G58390 AT1G58390 AT1G58400 AT1G58400 AT1G58400 AT1G58410 AT1G58410 AT1G58410 AT1G58410 AT1G58602 AT1G58602 AT1G58807 AT1G58807 AT1G58848 AT1G58848 AT1G59124 AT1G59124 AT1G59218 AT1G59218 AT1G59218 AT1G59620 AT1G59780 AT1G59780 AT5G35450 AT5G35450 AT5G43470 AT5G43470 AT5G43470 AT5G43470 AT5G48620 AT5G48620 AT5G48620 AT5G48620 AT5G48620 AT5G48620
fragaria_vesca FvH4_4g04072 FvH4_4g22931 FvH4_4g22931 FvH4_4g22940 FvH4_6g49940 FvH4_6g49940 FvH4_7g08100 FvH4_7g08870 FvH4_7g08870 FvH4_7g09280 FvH4_7g09290 FvH4_7g15190 FvH4_7g15200 FvH4_7g15240 FvH4_7g15240 FvH4_7g33440
malus_domestica MD00G1174600.v1.1 MD01G1113700.v1.1 MD03G1071300.v1.1 MD04G1041500.v1.1 MD04G1238000.v1.1 MD07G1029000.v1.1 MD07G1092100.v1.1 MD07G1092300.v1.1 MD07G1092600.v1.1 MD12G1256300.v1.1 MD13G1128200.v1.1 MD13G1128400.v1.1 MD17G1268000.v1.1
prunus_persica Prupe.1G041300_v2.0.a1 Prupe.1G041300_v2.0.a1 Prupe.1G042900_v2.0.a1 Prupe.1G043100_v2.0.a1 Prupe.1G043100_v2.0.a1 Prupe.1G043100_v2.0.a1 Prupe.1G043100_v2.0.a1 Prupe.1G043600_v2.0.a1 Prupe.1G043700_v2.0.a1 Prupe.1G043700_v2.0.a1 Prupe.1G043700_v2.0.a1 Prupe.1G043700_v2.0.a1 Prupe.1G043700_v2.0.a1 Prupe.1G043700_v2.0.a1 Prupe.1G046700_v2.0.a1 Prupe.1G047700_v2.0.a1 Prupe.1G047900_v2.0.a1 Prupe.1G048000_v2.0.a1 Prupe.1G048500_v2.0.a1 Prupe.1G048500_v2.0.a1 Prupe.1G049900_v2.0.a1 Prupe.1G051500_v2.0.a1 Prupe.1G051800_v2.0.a1 Prupe.1G051900_v2.0.a1 Prupe.1G052200_v2.0.a1 Prupe.1G194900_v2.0.a1 Prupe.1G217800_v2.0.a1 Prupe.1G217800_v2.0.a1 Prupe.1G217800_v2.0.a1 Prupe.1G217800_v2.0.a1 Prupe.1G217800_v2.0.a1 Prupe.1G217800_v2.0.a1 Prupe.1G217800_v2.0.a1 Prupe.1G217800_v2.0.a1 Prupe.1G217800_v2.0.a1 Prupe.1G217900_v2.0.a1 Prupe.1G217900_v2.0.a1 Prupe.1G217900_v2.0.a1 Prupe.1G217900_v2.0.a1 Prupe.1G217900_v2.0.a1 Prupe.2G117200_v2.0.a1 Prupe.2G117200_v2.0.a1 Prupe.2G117200_v2.0.a1 Prupe.2G117200_v2.0.a1 Prupe.2G117300_v2.0.a1 Prupe.2G117400_v2.0.a1 Prupe.2G117500_v2.0.a1 Prupe.2G117700_v2.0.a1 Prupe.2G118000_v2.0.a1 Prupe.2G118000_v2.0.a1 Prupe.2G118000_v2.0.a1 Prupe.2G118000_v2.0.a1 Prupe.2G118000_v2.0.a1 Prupe.2G118000_v2.0.a1 Prupe.2G118000_v2.0.a1 Prupe.2G119900_v2.0.a1
pyrus_communis pycom03g05750 pycom04g21110 pycom07g07530 pycom07g07590 pycom13g11110 pycom13g11140 pycom13g11160 pycom13g11170 pycom17g26680
rosa_chinensis RchiOBHm_Chr1g0341231 RchiOBHm_Chr1g0344091 RchiOBHm_Chr1g0344161 RchiOBHm_Chr1g0345001 RchiOBHm_Chr1g0345351 RchiOBHm_Chr1g0345371 RchiOBHm_Chr1g0345391 RchiOBHm_Chr1g0345531 RchiOBHm_Chr1g0345551 RchiOBHm_Chr1g0345571 RchiOBHm_Chr1g0345581 RchiOBHm_Chr1g0345661 RchiOBHm_Chr1g0345691 RchiOBHm_Chr1g0345761 RchiOBHm_Chr1g0345791 RchiOBHm_Chr1g0345821 RchiOBHm_Chr1g0345911 RchiOBHm_Chr1g0345941 RchiOBHm_Chr1g0357611 RchiOBHm_Chr1g0357911 RchiOBHm_Chr1g0357921 RchiOBHm_Chr1g0357931 RchiOBHm_Chr1g0357971 RchiOBHm_Chr1g0358121 RchiOBHm_Chr1g0383491 RchiOBHm_Chr1g0383511 RchiOBHm_Chr1g0383521 RchiOBHm_Chr3g0480581 RchiOBHm_Chr4g0394341 RchiOBHm_Chr4g0394361 RchiOBHm_Chr4g0394411 RchiOBHm_Chr4g0394441 RchiOBHm_Chr4g0407681 RchiOBHm_Chr4g0429191 RchiOBHm_Chr4g0429201 RchiOBHm_Chr4g0429211 RchiOBHm_Chr4g0429221 RchiOBHm_Chr6g0265091
rosa_laevigata RLG00000007088 RLG00000008700 RLG00000009190 RLG00000009702 RLG00000014210 RLG00000028829 RLG00000028833 RLG00000028837 RLG00000028838 RLG00000028921 RLG00000028926
rosa_multiflora Rmu_co8366955.1_g000001 Rmu_co8394205.1_g000001 Rmu_sc0000019.1_g000001 Rmu_sc0000054.1_g000031 Rmu_sc0000054.1_g000033 Rmu_sc0000200.1_g000002 Rmu_sc0000200.1_g000007 Rmu_sc0000200.1_g000018 Rmu_sc0000200.1_g000020 Rmu_sc0000200.1_g000021 Rmu_sc0000251.1_g000012 Rmu_sc0000251.1_g000015 Rmu_sc0000251.1_g000028 Rmu_sc0000253.1_g000015 Rmu_sc0000255.1_g000045 Rmu_sc0000255.1_g000046 Rmu_sc0001292.1_g000013 Rmu_sc0001292.1_g000018 Rmu_sc0001292.1_g000028 Rmu_sc0001292.1_g000029 Rmu_sc0001349.1_g000006 Rmu_sc0001349.1_g000014 Rmu_sc0001582.1_g000006 Rmu_sc0001582.1_g000018 Rmu_sc0001582.1_g000020 Rmu_sc0001692.1_g000007 Rmu_sc0001692.1_g000024 Rmu_sc0001692.1_g000026 Rmu_sc0001692.1_g000029 Rmu_sc0001692.1_g000030 Rmu_sc0001835.1_g000006 Rmu_sc0001835.1_g000010 Rmu_sc0002071.1_g000005 Rmu_sc0002276.1_g000002 Rmu_sc0002276.1_g000009 Rmu_sc0002329.1_g000018 Rmu_sc0002329.1_g000023 Rmu_sc0002329.1_g000025 Rmu_sc0002523.1_g000005 Rmu_sc0003573.1_g000005 Rmu_sc0003573.1_g000006 Rmu_sc0004099.1_g000021 Rmu_sc0005812.1_g000009 Rmu_sc0006271.1_g000002 Rmu_sc0006287.1_g000038 Rmu_sc0006439.1_g000008 Rmu_sc0007456.1_g000004 Rmu_sc0007581.1_g000009 Rmu_sc0007849.1_g000014 Rmu_sc0008115.1_g000012 Rmu_sc0010521.1_g000004 Rmu_sc0012471.1_g000017 Rmu_sc0014762.1_g000001 Rmu_sc0015150.1_g000003 Rmu_sc0017221.1_g000001 Rmu_sc0017299.1_g000001 Rmu_sc0017299.1_g000002 Rmu_sc0021457.1_g000001 Rmu_sc0022067.1_g000001 Rmu_sc0033275.1_g000001 Rmu_ssc0000245.1_g000012 Rmu_ssc0000359.1_g000035 Rmu_ssc0000359.1_g000067 Rmu_ssc0000359.1_g000072 Rmu_ssc0000434.1_g000005 Rmu_ssc0000434.1_g000007
rosa_roxburghii Rroxscaffold_4G00298420 Rroxscaffold_4G00298720 Rroxscaffold_4G00308990 Rroxscaffold_4G00309000 Rroxscaffold_4G00309010 Rroxscaffold_4G00309050 Rroxscaffold_4G00309070 Rroxscaffold_4G00309100 Rroxscaffold_4G00310030 Rroxscaffold_4G00310050 Rroxscaffold_4G00311880 Rroxscaffold_5G00339420 Rroxscaffold_5G00339450 Rroxscaffold_5G00344880 Rroxscaffold_5G00352270 Rroxscaffold_5G00362240 Rroxscaffold_5G00370840 Rroxscaffold_7G00202970
rosa_rugosa Rorug01G0159100.1 Rorug01G0159200.1 Rorug01G0171700 Rorug01G0171900 Rorug01G0172000 Rorug01G0191700 Rorug01G0191800 Rorug01G0256900 Rorug01G0257000 Rorug01G0257100 Rorug03G0198000 Rorug03G0366300.1 Rorug03G0366400.1 Rorug03G0366500.1 Rorug04G0081200 Rorug04G0081200 Rorug04G0081200 Rorug04G0233700 Rorug04G0233800 Rorug04G0233800 Rorug06G0013300
rosa_samantha Rh1CG138800 Rh1CG161300 Rh1CG173800 Rh1CG179800 Rh1CG180100 Rh1CG180500 Rh1CG180800 Rh1CG180900 Rh1CG194100 Rh1DG205100 Rh3AG236300 Rh4AG052000 Rh4AG052100 Rh4AG145900 Rh4AG289700 Rh4AG289800 Rh6AG136800 Rh6AG137300 Rh6BG133600 Rh6CG132000 Rh6CG134000 Rh6DG119300
rosa_wichuraiana Rw0G003420 Rw1G015500 Rw1G016110 Rw1G016180 Rw1G016210 Rw1G016230 Rw1G016280 Rw1G016440 Rw1G016450 Rw1G016460 Rw1G016480 Rw1G017490 Rw1G024140 Rw1G024160 Rw3G022530 Rw4G004140 Rw4G004190 Rw4G011850 Rw6G011800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 54
Acc65I GGTACC 1 cut(s) 1225
AccB1I GGYRCC 1 cut(s) 1225
AccB7I CCANNNNNTGG 1 cut(s) 1022
AccI GTMKAC 2 cut(s) 939, 2344
AccII CGCG 2 cut(s) 428, 1324
AccIII TCCGGA 3 cut(s) 1303, 1570, 2671
AciI CCGC 9 cut(s) 118, 613, 948, 1122, 1322, 1324, 1495, 2368, 2496
AclI AACGTT 2 cut(s) 90, 2246
AclWI GGATC 6 cut(s) 747, 1477, 1936, 1998, 2189, 2444
AcoI YGGCCR 1 cut(s) 1104
AcsI RAATTY 8 cut(s) 852, 967, 1317, 1591, 1960, 2004, 2223, 2487
AcuI CTGAAG 4 cut(s) 983, 996, 2681, 2684
AcvI CACGTG 1 cut(s) 2359
AfaI GTAC 4 cut(s) 281, 373, 1227, 2781
AfiI CCNNNNNNNGG 6 cut(s) 567, 877, 878, 1022, 2303, 2731
AflIII ACRYGT 1 cut(s) 2776
AhdI GACNNNNNGTC 1 cut(s) 1652
AhlI ACTAGT 1 cut(s) 514
AjnI CCWGG 2 cut(s) 408, 845
Alw21I GWGCWC 2 cut(s) 528, 1152
Alw26I GTCTC 5 cut(s) 28, 753, 1724, 2276, 2565
Alw44I GTGCAC 1 cut(s) 1148
AlwI GGATC 6 cut(s) 747, 1477, 1936, 1998, 2189, 2444
Ama87I CYCGRG 1 cut(s) 2258
Aor13HI TCCGGA 3 cut(s) 1303, 1570, 2671
AoxI GGCC 3 cut(s) 336, 843, 1104
ApaLI GTGCAC 1 cut(s) 1148
ApeKI GCWGC 5 cut(s) 891, 1771, 2083, 2253, 2380
ApoI RAATTY 8 cut(s) 852, 967, 1317, 1591, 1960, 2004, 2223, 2487
ArsI GACNNNNNNTTYG 2 cut(s) 2543, 2575
AseI ATTAAT 1 cut(s) 1437
Asp700I GAANNNNTTC 4 cut(s) 699, 1548, 2154, 2225
Asp718I GGTACC 1 cut(s) 1225
AspS9I GGNCC 3 cut(s) 43, 1725, 1835
AsuHPI GGTGA 3 cut(s) 398, 1600, 2230
AsuII TTCGAA 1 cut(s) 175
AvaI CYCGRG 1 cut(s) 2258
AvaII GGWCC 3 cut(s) 43, 1725, 1835
AxyI CCTNAGG 1 cut(s) 1230
BaeGI GKGCMC 1 cut(s) 1152
BaeI ACNNNNGTAYC 2 cut(s) 271, 304
BalI TGGCCA 1 cut(s) 1106
BanI GGYRCC 1 cut(s) 1225
BanII GRGCYC 3 cut(s) 416, 528, 2435
BarI GAAGNNNNNNTAC 2 cut(s) 2139, 2171
BauI CACGAG 1 cut(s) 1151
BbrPI CACGTG 1 cut(s) 2359
BbsI GAAGAC 2 cut(s) 944, 1648
Bbv12I GWGCWC 2 cut(s) 528, 1152
BbvI GCAGC 5 cut(s) 903, 1758, 2070, 2240, 2367
BceAI ACGGC 2 cut(s) 400, 2325
BcgI CGANNNNNNTGC 2 cut(s) 752, 786
BciT130I CCWGG 2 cut(s) 410, 847
BclI TGATCA 1 cut(s) 2791
BcoDI GTCTC 5 cut(s) 28, 753, 1724, 2276, 2565
BcuI ACTAGT 1 cut(s) 514
BfaI CTAG 4 cut(s) 348, 366, 515, 2622
BfuAI ACCTGC 1 cut(s) 54
BglII AGATCT 1 cut(s) 2152
BisI GCNGC 6 cut(s) 892, 1495, 1772, 2084, 2254, 2381
BlsI GCNGC 6 cut(s) 893, 1496, 1773, 2085, 2255, 2382
Bme1390I CCNGG 2 cut(s) 410, 847
Bme18I GGWCC 3 cut(s) 43, 1725, 1835
BmeRI GACNNNNNGTC 1 cut(s) 1652
BmeT110I CYCGRG 1 cut(s) 2258
BmgT120I GGNCC 3 cut(s) 43, 1725, 1835
BmiI GGNNCC 5 cut(s) 44, 1227, 1727, 1836, 2614
BmrFI CCNGG 2 cut(s) 410, 847
BmrI ACTGGG 1 cut(s) 2099
BmsI GCATC 5 cut(s) 111, 130, 202, 2661, 2711
BmuI ACTGGG 1 cut(s) 2099
BpiI GAAGAC 2 cut(s) 944, 1648
BplI GAGNNNNNCTC 2 cut(s) 1202, 1234
BpmI CTGGAG 1 cut(s) 1538
Bpu10I CCTNAGC 1 cut(s) 2036
Bpu14I TTCGAA 1 cut(s) 175
BpuEI CTTGAG 4 cut(s) 409, 2462, 2503, 2540
BsaAI YACGTR 1 cut(s) 2359
BsaJI CCNNGG 8 cut(s) 216, 409, 600, 680, 962, 1322, 2539, 2572
BsaWI WCCGGW 4 cut(s) 1303, 1475, 1570, 2671
Bsc4I CCNNNNNNNGG 6 cut(s) 567, 877, 878, 1022, 2303, 2731
Bse1I ACTGG 4 cut(s) 1338, 1636, 2094, 2469
Bse21I CCTNAGG 1 cut(s) 1230
Bse3DI GCAATG 3 cut(s) 131, 622, 2074
BseAI TCCGGA 3 cut(s) 1303, 1570, 2671
BseBI CCWGG 2 cut(s) 410, 847
BseDI CCNNGG 8 cut(s) 216, 409, 600, 680, 962, 1322, 2539, 2572
BseLI CCNNNNNNNGG 6 cut(s) 567, 877, 878, 1022, 2303, 2731
BseMI GCAATG 3 cut(s) 131, 622, 2074
BseMII CTCAG 6 cut(s) 318, 1221, 1729, 1735, 2188, 2672
BseNI ACTGG 4 cut(s) 1338, 1636, 2094, 2469
BseRI GAGGAG 2 cut(s) 746, 2558
BseSI GKGCMC 1 cut(s) 1152
BseXI GCAGC 5 cut(s) 903, 1758, 2070, 2240, 2367
BseYI CCCAGC 1 cut(s) 2513
BsgI GTGCAG 1 cut(s) 910
Bsh1236I CGCG 2 cut(s) 428, 1324
Bsh1285I CGRYCG 2 cut(s) 298, 550
BshFI GGCC 3 cut(s) 338, 845, 1106
BshNI GGYRCC 1 cut(s) 1225
BsiEI CGRYCG 2 cut(s) 298, 550
BsiHKAI GWGCWC 2 cut(s) 528, 1152
BsiHKCI CYCGRG 1 cut(s) 2258
BsiSI CCGG 5 cut(s) 1304, 1476, 1571, 2384, 2672
BslFI GGGAC 8 cut(s) 56, 244, 1220, 1711, 1848, 1880, 2217, 2248
BslI CCNNNNNNNGG 6 cut(s) 567, 877, 878, 1022, 2303, 2731
BsmAI GTCTC 5 cut(s) 28, 753, 1724, 2276, 2565
BsmBI CGTCTC 1 cut(s) 753
BsmFI GGGAC 8 cut(s) 56, 244, 1220, 1711, 1848, 1880, 2217, 2248
BsnI GGCC 3 cut(s) 338, 845, 1106
BsoBI CYCGRG 1 cut(s) 2258
Bsp119I TTCGAA 1 cut(s) 175
Bsp1286I GDGCHC 4 cut(s) 416, 528, 1152, 2435
Bsp13I TCCGGA 3 cut(s) 1303, 1570, 2671
BspACI CCGC 9 cut(s) 118, 613, 948, 1122, 1322, 1324, 1495, 2368, 2496
BspANI GGCC 3 cut(s) 338, 845, 1106
BspCNI CTCAG 6 cut(s) 319, 1222, 1728, 1734, 2187, 2673
BspEI TCCGGA 3 cut(s) 1303, 1570, 2671
BspFNI CGCG 2 cut(s) 428, 1324
BspHI TCATGA 4 cut(s) 528, 955, 1252, 1375
BspLI GGNNCC 5 cut(s) 44, 1227, 1727, 1836, 2614
BspMI ACCTGC 1 cut(s) 54
BspPI GGATC 6 cut(s) 747, 1477, 1936, 1998, 2189, 2444
BspQI GCTCTTC 1 cut(s) 759
BspT104I TTCGAA 1 cut(s) 175
BspT107I GGYRCC 1 cut(s) 1225
BsrDI GCAATG 3 cut(s) 131, 622, 2074
BsrI ACTGG 4 cut(s) 1338, 1636, 2094, 2469
BssECI CCNNGG 8 cut(s) 216, 409, 600, 680, 962, 1322, 2539, 2572
BssSI CACGAG 1 cut(s) 1151
BssT1I CCWWGG 3 cut(s) 600, 680, 2539
Bst2BI CACGAG 1 cut(s) 1151
Bst2UI CCWGG 2 cut(s) 410, 847
Bst4CI ACNGT 6 cut(s) 548, 1147, 1225, 1839, 1999, 2784
Bst6I CTCTTC 2 cut(s) 759, 975
BstBAI YACGTR 1 cut(s) 2359
BstBI TTCGAA 1 cut(s) 175
BstC8I GCNNGC 6 cut(s) 771, 1093, 1097, 1197, 1610, 1747
BstDSI CCRYGG 1 cut(s) 1322
BstENI CCTNNNNNAGG 1 cut(s) 2729
BstFNI CGCG 2 cut(s) 428, 1324
BstMAI GTCTC 5 cut(s) 28, 753, 1724, 2276, 2565
BstMCI CGRYCG 2 cut(s) 298, 550
BstMWI GCNNNNNNNGC 2 cut(s) 212, 344
BstNI CCWGG 2 cut(s) 410, 847
BstNSI RCATGY 2 cut(s) 1612, 2780
BstSCI CCNGG 2 cut(s) 408, 845
BstSLI GKGCMC 1 cut(s) 1152
BstUI CGCG 2 cut(s) 428, 1324
BstV1I GCAGC 5 cut(s) 903, 1758, 2070, 2240, 2367
BstV2I GAAGAC 2 cut(s) 944, 1648
BstX2I RGATCY 3 cut(s) 1990, 2152, 2449
BstXI CCANNNNNNTGG 1 cut(s) 488
BstYI RGATCY 3 cut(s) 1990, 2152, 2449
Bsu36I CCTNAGG 1 cut(s) 1230
BsuRI GGCC 3 cut(s) 338, 845, 1106
BtgI CCRYGG 1 cut(s) 1322
BtsIMutI CAGTG 3 cut(s) 1152, 2004, 2462
BveI ACCTGC 1 cut(s) 54
Cac8I GCNNGC 6 cut(s) 771, 1093, 1097, 1197, 1610, 1747
CciI TCATGA 4 cut(s) 528, 955, 1252, 1375
Cfr13I GGNCC 3 cut(s) 43, 1725, 1835
Cfr42I CCGCGG 1 cut(s) 1325
Csp6I GTAC 4 cut(s) 280, 372, 1226, 2780
CspCI CAANNNNNGTGG 4 cut(s) 434, 469, 2044, 2079
CviQI GTAC 4 cut(s) 280, 372, 1226, 2780
DraI TTTAAA 1 cut(s) 1959
DriI GACNNNNNGTC 1 cut(s) 1652
EaeI YGGCCR 1 cut(s) 1104
Eam1104I CTCTTC 2 cut(s) 759, 975
Eam1105I GACNNNNNGTC 1 cut(s) 1652
EarI CTCTTC 2 cut(s) 759, 975
EciI GGCGGA 3 cut(s) 133, 1137, 2383
Ecl136II GAGCTC 1 cut(s) 526
Eco130I CCWWGG 3 cut(s) 600, 680, 2539
Eco147I AGGCCT 1 cut(s) 845
Eco24I GRGCYC 3 cut(s) 416, 528, 2435
Eco32I GATATC 2 cut(s) 1162, 1861
Eco47I GGWCC 3 cut(s) 43, 1725, 1835
Eco53kI GAGCTC 1 cut(s) 526
Eco57I CTGAAG 4 cut(s) 983, 996, 2681, 2684
Eco72I CACGTG 1 cut(s) 2359
Eco81I CCTNAGG 1 cut(s) 1230
Eco88I CYCGRG 1 cut(s) 2258
EcoICRI GAGCTC 1 cut(s) 526
EcoNI CCTNNNNNAGG 1 cut(s) 2729
EcoO109I RGGNCCY 2 cut(s) 43, 1725
EcoRI GAATTC 1 cut(s) 1591
EcoRII CCWGG 2 cut(s) 408, 845
EcoRV GATATC 2 cut(s) 1162, 1861
EcoT14I CCWWGG 3 cut(s) 600, 680, 2539
EcoT38I GRGCYC 3 cut(s) 416, 528, 2435
ErhI CCWWGG 3 cut(s) 600, 680, 2539
Esp3I CGTCTC 1 cut(s) 753
FalI AAGNNNNNCTT 2 cut(s) 268, 300
FaqI GGGAC 8 cut(s) 56, 244, 1220, 1711, 1848, 1880, 2217, 2248
FbaI TGATCA 1 cut(s) 2791
FblI GTMKAC 2 cut(s) 939, 2344
Fnu4HI GCNGC 6 cut(s) 892, 1495, 1772, 2084, 2254, 2381
FriOI GRGCYC 3 cut(s) 416, 528, 2435
Fsp4HI GCNGC 6 cut(s) 892, 1495, 1772, 2084, 2254, 2381
FspBI CTAG 4 cut(s) 348, 366, 515, 2622
GluI GCNGC 6 cut(s) 892, 1495, 1772, 2084, 2254, 2381
GsaI CCCAGC 1 cut(s) 2517
GsuI CTGGAG 1 cut(s) 1538
HaeIII GGCC 3 cut(s) 338, 845, 1106
HapII CCGG 5 cut(s) 1304, 1476, 1571, 2384, 2672
HincII GTYRAC 3 cut(s) 2166, 2421, 2737
HindII GTYRAC 3 cut(s) 2166, 2421, 2737
HindIII AAGCTT 2 cut(s) 779, 1914
HinfI GANTC 7 cut(s) 105, 351, 1294, 1706, 2261, 2668, 2675
HpaI GTTAAC 1 cut(s) 2421
HpaII CCGG 5 cut(s) 1304, 1476, 1571, 2384, 2672
HphI GGTGA 3 cut(s) 398, 1600, 2230
Hpy99I CGWCG 3 cut(s) 421, 727, 880
HpyCH4III ACNGT 6 cut(s) 548, 1147, 1225, 1839, 1999, 2784
HpyCH4IV ACGT 4 cut(s) 90, 2145, 2246, 2358
HpyF10VI GCNNNNNNNGC 2 cut(s) 212, 344
HpySE526I ACGT 4 cut(s) 90, 2145, 2246, 2358
Kpn2I TCCGGA 3 cut(s) 1303, 1570, 2671
KpnI GGTACC 1 cut(s) 1229
Ksp22I TGATCA 1 cut(s) 2791
KspAI GTTAAC 1 cut(s) 2421
KspI CCGCGG 1 cut(s) 1325
LguI GCTCTTC 1 cut(s) 759
LmnI GCTCC 4 cut(s) 523, 1044, 1057, 2612
Lsp1109I GCAGC 5 cut(s) 903, 1758, 2070, 2240, 2367
LweI GCATC 5 cut(s) 111, 130, 202, 2661, 2711
MaeI CTAG 4 cut(s) 348, 366, 515, 2622
MaeII ACGT 4 cut(s) 90, 2145, 2246, 2358
MaeIII GTNAC 6 cut(s) 69, 1295, 1427, 1514, 1848, 2141
MfeI CAATTG 1 cut(s) 446
MflI RGATCY 3 cut(s) 1990, 2152, 2449
MhlI GDGCHC 4 cut(s) 416, 528, 1152, 2435
MlsI TGGCCA 1 cut(s) 1106
MluNI TGGCCA 1 cut(s) 1106
MlyI GAGTC 3 cut(s) 99, 1303, 2270
MmeI TCCRAC 1 cut(s) 1438
Mox20I TGGCCA 1 cut(s) 1106
MroI TCCGGA 3 cut(s) 1303, 1570, 2671
MroXI GAANNNNTTC 4 cut(s) 699, 1548, 2154, 2225
MscI TGGCCA 1 cut(s) 1106
MslI CAYNNNNRTG 1 cut(s) 1112
Msp20I TGGCCA 1 cut(s) 1106
MspA1I CMGCKG 1 cut(s) 1324
MspI CCGG 5 cut(s) 1304, 1476, 1571, 2384, 2672
MspR9I CCNGG 2 cut(s) 410, 847
MunI CAATTG 1 cut(s) 446
MvaI CCWGG 2 cut(s) 410, 847
MvnI CGCG 2 cut(s) 428, 1324
MwoI GCNNNNNNNGC 2 cut(s) 212, 344
NlaIV GGNNCC 5 cut(s) 44, 1227, 1727, 1836, 2614
NmeAIII GCCGAG 2 cut(s) 241, 987
NmuCI GTSAC 3 cut(s) 1295, 1514, 2141
NspI RCATGY 2 cut(s) 1612, 2780
NspV TTCGAA 1 cut(s) 175
PaeI GCATGC 1 cut(s) 1612
PaeR7I CTCGAG 1 cut(s) 2258
PagI TCATGA 4 cut(s) 528, 955, 1252, 1375
PceI AGGCCT 1 cut(s) 845
PciI ACATGT 1 cut(s) 2776
PciSI GCTCTTC 1 cut(s) 759
PcsI WCGNNNNNNNCGW 2 cut(s) 425, 628
PdmI GAANNNNTTC 4 cut(s) 699, 1548, 2154, 2225
PfeI GAWTC 4 cut(s) 351, 1706, 2668, 2675
PflMI CCANNNNNTGG 1 cut(s) 1022
PkrI GCNGC 6 cut(s) 893, 1496, 1773, 2085, 2255, 2382
PleI GAGTC 3 cut(s) 99, 1302, 2269
PmaCI CACGTG 1 cut(s) 2359
PmlI CACGTG 1 cut(s) 2359
PpsI GAGTC 3 cut(s) 99, 1302, 2269
Ppu21I YACGTR 1 cut(s) 2359
PpuMI RGGWCCY 2 cut(s) 43, 1725
PscI ACATGT 1 cut(s) 2776
PshBI ATTAAT 1 cut(s) 1437
Psp124BI GAGCTC 1 cut(s) 528
Psp1406I AACGTT 2 cut(s) 90, 2246
Psp5II RGGWCCY 2 cut(s) 43, 1725
Psp6I CCWGG 2 cut(s) 408, 845
PspCI CACGTG 1 cut(s) 2359
PspFI CCCAGC 1 cut(s) 2513
PspGI CCWGG 2 cut(s) 408, 845
PspN4I GGNNCC 5 cut(s) 44, 1227, 1727, 1836, 2614
PspPI GGNCC 3 cut(s) 43, 1725, 1835
PspPPI RGGWCCY 2 cut(s) 43, 1725
PspXI VCTCGAGB 1 cut(s) 2258
PsuI RGATCY 3 cut(s) 1990, 2152, 2449
RsaI GTAC 4 cut(s) 281, 373, 1227, 2781
RsaNI GTAC 4 cut(s) 280, 372, 1226, 2780
RseI CAYNNNNRTG 1 cut(s) 1112
SacI GAGCTC 1 cut(s) 528
SacII CCGCGG 1 cut(s) 1325
SapI GCTCTTC 1 cut(s) 759
SatI GCNGC 6 cut(s) 892, 1495, 1772, 2084, 2254, 2381
Sau96I GGNCC 3 cut(s) 43, 1725, 1835
SchI GAGTC 3 cut(s) 99, 1303, 2270
ScrFI CCNGG 2 cut(s) 410, 847
SduI GDGCHC 4 cut(s) 416, 528, 1152, 2435
SfaNI GCATC 5 cut(s) 111, 130, 202, 2661, 2711
Sfr274I CTCGAG 1 cut(s) 2258
Sfr303I CCGCGG 1 cut(s) 1325
SfuI TTCGAA 1 cut(s) 175
SgrBI CCGCGG 1 cut(s) 1325
SinI GGWCC 3 cut(s) 43, 1725, 1835
SlaI CTCGAG 1 cut(s) 2258
SmiMI CAYNNNNRTG 1 cut(s) 1112
SmlI CTYRAG 5 cut(s) 388, 2258, 2441, 2482, 2555
SmoI CTYRAG 5 cut(s) 388, 2258, 2441, 2482, 2555
SpeI ACTAGT 1 cut(s) 514
SphI GCATGC 1 cut(s) 1612
SseBI AGGCCT 1 cut(s) 845
SsiI CCGC 9 cut(s) 118, 613, 948, 1122, 1322, 1324, 1495, 2368, 2496
SspI AATATT 2 cut(s) 809, 900
SspMI CTAG 4 cut(s) 348, 366, 515, 2622
SstI GAGCTC 1 cut(s) 528
StuI AGGCCT 1 cut(s) 845
StyD4I CCNGG 2 cut(s) 408, 845
StyI CCWWGG 3 cut(s) 600, 680, 2539
TaaI ACNGT 6 cut(s) 548, 1147, 1225, 1839, 1999, 2784
TaiI ACGT 4 cut(s) 93, 2148, 2249, 2361
TaqII GACCGA 2 cut(s) 312, 957
TatI WGTACW 1 cut(s) 371
TauI GCSGC 1 cut(s) 1497
TfiI GAWTC 4 cut(s) 351, 1706, 2668, 2675
TscAI CASTG 3 cut(s) 1152, 2004, 2469
TseFI GTSAC 3 cut(s) 1295, 1514, 2141
TseI GCWGC 5 cut(s) 891, 1771, 2083, 2253, 2380
Tsp45I GTSAC 3 cut(s) 1295, 1514, 2141
TspDTI ATGAA 8 cut(s) 545, 549, 944, 1392, 1407, 1553, 1590, 1857
TspGWI ACGGA 1 cut(s) 711
TspRI CASTG 3 cut(s) 1152, 2004, 2469
Van91I CCANNNNNTGG 1 cut(s) 1022
VneI GTGCAC 1 cut(s) 1148
VpaK11BI GGWCC 3 cut(s) 43, 1725, 1835
VspI ATTAAT 1 cut(s) 1437
XagI CCTNNNNNAGG 1 cut(s) 2729
XapI RAATTY 8 cut(s) 852, 967, 1317, 1591, 1960, 2004, 2223, 2487
XceI RCATGY 2 cut(s) 1612, 2780
XcmI CCANNNNNNNNNTGG 2 cut(s) 568, 687
XhoI CTCGAG 1 cut(s) 2258
XmiI GTMKAC 2 cut(s) 939, 2344
XmnI GAANNNNTTC 4 cut(s) 699, 1548, 2154, 2225
XspI CTAG 4 cut(s) 348, 366, 515, 2622
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.