MD04G1238000.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000069
Physical Location & Seq
Reverse (-)
31613017 .. 31616624
3608 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1238000.v1.1.491

Sequence Viewer

Length: 2808 bp
ATGGCAGAGTCTGTGGTATACTTTGTTATTGAAAGGCTTGGGGACCTGCTGATTGAAAAAGCCACCTTCTTACACGGGGTCAAGCAAAACGTCGAGCAGATCCGAGTCGAACTGAGTCGGATGCAATGCTTCTTGAAAGATGCCGATAAGAGGCAAGACGAGGATGATAGCATTCGGAATTGGGTTTCCGAGATTAGAGAAGTTGCTTATGATGCAGAAGATGTCATTGGAAGTTTCACCATCAAAATTTCACCACCAATTTCCAATCCGTTAAAAAGGTATGCTTGTTTTTTCAACCGAGCTTCAAACCTTTATGATGTTGGGTCTGAGATTGAGGCCATCAAAGCTAGAATTTCTGACCTTACTAGAAGTACACAAACTTACGGCTTGACTGTAGTAAGAGATCATCAAGGCTCGTCGAGTATTGCGTTTGAGAAGCAAAGACAATTGAGGTGGTCTTATTCCCATGTTATTGATGACCATATCGTTGGTTTACAAGGAAGCATAAACGAACTAGTGGCGGAGCTCATGAATGAAGAAAAACACGGTCGAGTTGTGTCCATTTGTGGGATGGGAGGTTTGGGGAAGACCACTCTTGCCAAGGAAATTTACCGCAATGATCATGTTAGGCGTTATTTTGACGCTTCTGCGTGGGCTTATATATCTCAACAATGCAAACCAAGAGATGTTTGGGAAGGCATTCTGATTAAGCTAACCTCTCCATCGAAAGAGGAGAGGGATCACATTTTGAAATTGAGAGACGAAGAGCTTGCAAAGAAGCTTTATCAAGTTCAAATAGAGAAGAAATATTTGGTGGTTCTTGATGACATTTGGTCGATTGAGGCCTGGAAAATTCTAAGTCCTGCCTTTCCATCGTCGGGAAAAGGGCGCAGCAGAATATTGCTTACAACTCGTAATAAGGACTTAGCTTCCTTTGTAGACCGAAGCGGCCTTCATGAGCCGAGGAACTTAACTGAAGAAGAGGGTTGGGAGTTGCTTCAGAAGAAGGCATTTCCAAGAAATGGTGATCCAGATTTTATCAGAAGCAAAGATAAGGAGCAGTTAGGGAGGGAAATGGTGAAAAAGTGTGCTGGCTTGCCTTTGGCCATTGTTGTGCTTGGAGGACTTTTGGCCACTAAAGAAACAGTGCACGAGTGGGATATCGTCCTCAGAGATATTCTTTCGTATCTAAAGCGAGCCAAAGGGCATGAGCAACACTCTACGGTACCTGAGGTTTTGGCTTTGAGTTATCATGATTTGCCTTTTATATTGAAACCCTGTTTCCTTTATTTGAGCCATTTTCCGGAAGATTTTGAAATTCCGAGGAGAAAACTGGTTCAATTATGGATTGCAGAAGGCATTGTATCGCCACACCATGAAGCAGAGGGAGATGAAACAATAGAGGATGTTGCGGAACGCTACTTGGGTTACTTGATTAATAGGTGCATGGTTCAAGTGGGAGCTCTCAGCTCAACTGGAAGAATTAAAACTTGCCGCCTCCATGATCTTATGCGTGACTTGTGCTTGTCGAAAGCAAAGCAGGAAAATTTTCTCCAGACTGTTCGCTGTTTGGATGAAGGTATGATGGTGGATTCTTCTTCTTCTTCTCGCATGCTTTCTGAAGCAAAATCAACTGGTAAAACTCGCAGACTTGCTGTCTTCTTACCTTCAGATGTCAATAATCTTATCCCATCTAAGTACAAAGAAGATTCCAACCTCAGTCTCAGGTCACTTATATATTTCCATGCAAGCAAGTGTAGATTGGTAAGTTGGCAGCTTACAAAAACAATTTTGGAGTTCAAAATGCTCAAAGTTCTCGATCTTGAAGGTGTCAAGGGACCATACGAAAAGTTACCTAAAGATATCGGGGGATTGGTTCAGTTGCAGTTTCTAAGTTTGAAGAAAACACATATACAAGCATTGCCATCATCCATCGGCAATTTGATCCACTTGAAAACTCTAAATTTGCAAACCATAAGCAAATTGAGTTGGGACCCAACAGTGCAAATTCCGAATGTGATTTGGAAGATGGAAGGGCTAAGGCATTTATATCTTCCCAAGTGGTGTGGCAATGCAGTTGATAAGCTGCAACTGGGAAATCTGATCAACTTGCAGACACTCGTAAACTTTCCTGCAAACAAATGCGACGTAGAAGATCTTCGCAAGTTGACCAATCTTAGAAAATTGGTGTTGAATGATCCAAAACATTTCAATAATTTGTTGAAAATTGTCAATCCCCAAAACAGAACGTTAAGCTACCTTGAGTCTTTGTCCTTGACAAGTGAGACACTTTCATTCCCTGATGAGGTTGTTGATGTTACACAAGTAATGTTAAGCTGTCGTCGTCTTAAAAAATTGCATGTGGAAGGGCGGATAGAGAAGCTGCCAGAATACCATCAGTTCCCTCCAAACCTTGCCAAGTTAACATTGTGGGGCTCAAAACTTGAGGAAGATCCAATGCCGACACTGGAAAAACTGCCGAACTTGAGAATTTTAAGCGGATGGCAAATGTTCATGGGGAAGAAAATGGTCTGCTCTAACCAAGGTTTCCCAAAGCTCAAGTCTCTACTCCTCCGAGGGTTTTCTAAGTTAGAGGATTGGACGATGGAAGTAGGAGCCATGCCTAGTCTTTGTCGTTTGGAAATATCAAATTGCACCAAACTGAAGACGATTCCGGATTCTCTGAGGTTTGTGAAAACACTTCAGGAATTGGAGATATACGGTTGCTTATTCAAGATCAACATTGGAAGTGAAGGAGAGGATTTTTACAAAGTGCAACATGTACCGTCCATTGTAGTACGCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

936

Amino Acids

107.0

Weight (kDa)

8.72

Isoelectric Point (pI)

46.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 5 - 84 5e-26 Rx N-terminal domain
NB-ARC PF00931 167 - 341 9.4e-42 NB-ARC domain
WHD_DRP PF23559 434 - 508 1.3e-19 Disease resistance protein Winged helix domain
LRR_14 PF23598 575 - 888 4.4e-37 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000069)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10920 AT1G10920 AT1G10920 AT1G10920 AT1G10920 AT1G50180 AT1G53350 AT1G58390 AT1G58390 AT1G58400 AT1G58400 AT1G58400 AT1G58410 AT1G58410 AT1G58410 AT1G58410 AT1G58602 AT1G58602 AT1G58807 AT1G58807 AT1G58848 AT1G58848 AT1G59124 AT1G59124 AT1G59218 AT1G59218 AT1G59218 AT1G59620 AT1G59780 AT1G59780 AT5G35450 AT5G35450 AT5G43470 AT5G43470 AT5G43470 AT5G43470 AT5G48620 AT5G48620 AT5G48620 AT5G48620 AT5G48620 AT5G48620
fragaria_vesca FvH4_4g04072 FvH4_4g22931 FvH4_4g22931 FvH4_4g22940 FvH4_6g49940 FvH4_6g49940 FvH4_7g08100 FvH4_7g08870 FvH4_7g08870 FvH4_7g09280 FvH4_7g09290 FvH4_7g15190 FvH4_7g15200 FvH4_7g15240 FvH4_7g15240 FvH4_7g33440
malus_domestica MD00G1174600.v1.1 MD01G1113700.v1.1 MD03G1071300.v1.1 MD04G1041500.v1.1 MD04G1238000.v1.1 MD07G1029000.v1.1 MD07G1092100.v1.1 MD07G1092300.v1.1 MD07G1092600.v1.1 MD12G1256300.v1.1 MD13G1128200.v1.1 MD13G1128400.v1.1 MD17G1268000.v1.1
prunus_persica Prupe.1G041300_v2.0.a1 Prupe.1G041300_v2.0.a1 Prupe.1G042900_v2.0.a1 Prupe.1G043100_v2.0.a1 Prupe.1G043100_v2.0.a1 Prupe.1G043100_v2.0.a1 Prupe.1G043100_v2.0.a1 Prupe.1G043600_v2.0.a1 Prupe.1G043700_v2.0.a1 Prupe.1G043700_v2.0.a1 Prupe.1G043700_v2.0.a1 Prupe.1G043700_v2.0.a1 Prupe.1G043700_v2.0.a1 Prupe.1G043700_v2.0.a1 Prupe.1G046700_v2.0.a1 Prupe.1G047700_v2.0.a1 Prupe.1G047900_v2.0.a1 Prupe.1G048000_v2.0.a1 Prupe.1G048500_v2.0.a1 Prupe.1G048500_v2.0.a1 Prupe.1G049900_v2.0.a1 Prupe.1G051500_v2.0.a1 Prupe.1G051800_v2.0.a1 Prupe.1G051900_v2.0.a1 Prupe.1G052200_v2.0.a1 Prupe.1G194900_v2.0.a1 Prupe.1G217800_v2.0.a1 Prupe.1G217800_v2.0.a1 Prupe.1G217800_v2.0.a1 Prupe.1G217800_v2.0.a1 Prupe.1G217800_v2.0.a1 Prupe.1G217800_v2.0.a1 Prupe.1G217800_v2.0.a1 Prupe.1G217800_v2.0.a1 Prupe.1G217800_v2.0.a1 Prupe.1G217900_v2.0.a1 Prupe.1G217900_v2.0.a1 Prupe.1G217900_v2.0.a1 Prupe.1G217900_v2.0.a1 Prupe.1G217900_v2.0.a1 Prupe.2G117200_v2.0.a1 Prupe.2G117200_v2.0.a1 Prupe.2G117200_v2.0.a1 Prupe.2G117200_v2.0.a1 Prupe.2G117300_v2.0.a1 Prupe.2G117400_v2.0.a1 Prupe.2G117500_v2.0.a1 Prupe.2G117700_v2.0.a1 Prupe.2G118000_v2.0.a1 Prupe.2G118000_v2.0.a1 Prupe.2G118000_v2.0.a1 Prupe.2G118000_v2.0.a1 Prupe.2G118000_v2.0.a1 Prupe.2G118000_v2.0.a1 Prupe.2G118000_v2.0.a1 Prupe.2G119900_v2.0.a1
pyrus_communis pycom03g05750 pycom04g21110 pycom07g07530 pycom07g07590 pycom13g11110 pycom13g11140 pycom13g11160 pycom13g11170 pycom17g26680
rosa_chinensis RchiOBHm_Chr1g0341231 RchiOBHm_Chr1g0344091 RchiOBHm_Chr1g0344161 RchiOBHm_Chr1g0345001 RchiOBHm_Chr1g0345351 RchiOBHm_Chr1g0345371 RchiOBHm_Chr1g0345391 RchiOBHm_Chr1g0345531 RchiOBHm_Chr1g0345551 RchiOBHm_Chr1g0345571 RchiOBHm_Chr1g0345581 RchiOBHm_Chr1g0345661 RchiOBHm_Chr1g0345691 RchiOBHm_Chr1g0345761 RchiOBHm_Chr1g0345791 RchiOBHm_Chr1g0345821 RchiOBHm_Chr1g0345911 RchiOBHm_Chr1g0345941 RchiOBHm_Chr1g0357611 RchiOBHm_Chr1g0357911 RchiOBHm_Chr1g0357921 RchiOBHm_Chr1g0357931 RchiOBHm_Chr1g0357971 RchiOBHm_Chr1g0358121 RchiOBHm_Chr1g0383491 RchiOBHm_Chr1g0383511 RchiOBHm_Chr1g0383521 RchiOBHm_Chr3g0480581 RchiOBHm_Chr4g0394341 RchiOBHm_Chr4g0394361 RchiOBHm_Chr4g0394411 RchiOBHm_Chr4g0394441 RchiOBHm_Chr4g0407681 RchiOBHm_Chr4g0429191 RchiOBHm_Chr4g0429201 RchiOBHm_Chr4g0429211 RchiOBHm_Chr4g0429221 RchiOBHm_Chr6g0265091
rosa_laevigata RLG00000007088 RLG00000008700 RLG00000009190 RLG00000009702 RLG00000014210 RLG00000028829 RLG00000028833 RLG00000028837 RLG00000028838 RLG00000028921 RLG00000028926
rosa_multiflora Rmu_co8366955.1_g000001 Rmu_co8394205.1_g000001 Rmu_sc0000019.1_g000001 Rmu_sc0000054.1_g000031 Rmu_sc0000054.1_g000033 Rmu_sc0000200.1_g000002 Rmu_sc0000200.1_g000007 Rmu_sc0000200.1_g000018 Rmu_sc0000200.1_g000020 Rmu_sc0000200.1_g000021 Rmu_sc0000251.1_g000012 Rmu_sc0000251.1_g000015 Rmu_sc0000251.1_g000028 Rmu_sc0000253.1_g000015 Rmu_sc0000255.1_g000045 Rmu_sc0000255.1_g000046 Rmu_sc0001292.1_g000013 Rmu_sc0001292.1_g000018 Rmu_sc0001292.1_g000028 Rmu_sc0001292.1_g000029 Rmu_sc0001349.1_g000006 Rmu_sc0001349.1_g000014 Rmu_sc0001582.1_g000006 Rmu_sc0001582.1_g000018 Rmu_sc0001582.1_g000020 Rmu_sc0001692.1_g000007 Rmu_sc0001692.1_g000024 Rmu_sc0001692.1_g000026 Rmu_sc0001692.1_g000029 Rmu_sc0001692.1_g000030 Rmu_sc0001835.1_g000006 Rmu_sc0001835.1_g000010 Rmu_sc0002071.1_g000005 Rmu_sc0002276.1_g000002 Rmu_sc0002276.1_g000009 Rmu_sc0002329.1_g000018 Rmu_sc0002329.1_g000023 Rmu_sc0002329.1_g000025 Rmu_sc0002523.1_g000005 Rmu_sc0003573.1_g000005 Rmu_sc0003573.1_g000006 Rmu_sc0004099.1_g000021 Rmu_sc0005812.1_g000009 Rmu_sc0006271.1_g000002 Rmu_sc0006287.1_g000038 Rmu_sc0006439.1_g000008 Rmu_sc0007456.1_g000004 Rmu_sc0007581.1_g000009 Rmu_sc0007849.1_g000014 Rmu_sc0008115.1_g000012 Rmu_sc0010521.1_g000004 Rmu_sc0012471.1_g000017 Rmu_sc0014762.1_g000001 Rmu_sc0015150.1_g000003 Rmu_sc0017221.1_g000001 Rmu_sc0017299.1_g000001 Rmu_sc0017299.1_g000002 Rmu_sc0021457.1_g000001 Rmu_sc0022067.1_g000001 Rmu_sc0033275.1_g000001 Rmu_ssc0000245.1_g000012 Rmu_ssc0000359.1_g000035 Rmu_ssc0000359.1_g000067 Rmu_ssc0000359.1_g000072 Rmu_ssc0000434.1_g000005 Rmu_ssc0000434.1_g000007
rosa_roxburghii Rroxscaffold_4G00298420 Rroxscaffold_4G00298720 Rroxscaffold_4G00308990 Rroxscaffold_4G00309000 Rroxscaffold_4G00309010 Rroxscaffold_4G00309050 Rroxscaffold_4G00309070 Rroxscaffold_4G00309100 Rroxscaffold_4G00310030 Rroxscaffold_4G00310050 Rroxscaffold_4G00311880 Rroxscaffold_5G00339420 Rroxscaffold_5G00339450 Rroxscaffold_5G00344880 Rroxscaffold_5G00352270 Rroxscaffold_5G00362240 Rroxscaffold_5G00370840 Rroxscaffold_7G00202970
rosa_rugosa Rorug01G0159100.1 Rorug01G0159200.1 Rorug01G0171700 Rorug01G0171900 Rorug01G0172000 Rorug01G0191700 Rorug01G0191800 Rorug01G0256900 Rorug01G0257000 Rorug01G0257100 Rorug03G0198000 Rorug03G0366300.1 Rorug03G0366400.1 Rorug03G0366500.1 Rorug04G0081200 Rorug04G0081200 Rorug04G0081200 Rorug04G0233700 Rorug04G0233800 Rorug04G0233800 Rorug06G0013300
rosa_samantha Rh1CG138800 Rh1CG161300 Rh1CG173800 Rh1CG179800 Rh1CG180100 Rh1CG180500 Rh1CG180800 Rh1CG180900 Rh1CG194100 Rh1DG205100 Rh3AG236300 Rh4AG052000 Rh4AG052100 Rh4AG145900 Rh4AG289700 Rh4AG289800 Rh6AG136800 Rh6AG137300 Rh6BG133600 Rh6CG132000 Rh6CG134000 Rh6DG119300
rosa_wichuraiana Rw0G003420 Rw1G015500 Rw1G016110 Rw1G016180 Rw1G016210 Rw1G016230 Rw1G016280 Rw1G016440 Rw1G016450 Rw1G016460 Rw1G016480 Rw1G017490 Rw1G024140 Rw1G024160 Rw3G022530 Rw4G004140 Rw4G004190 Rw4G011850 Rw6G011800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 54
Acc65I GGTACC 1 cut(s) 1225
AccB1I GGYRCC 1 cut(s) 1225
AccB7I CCANNNNNTGG 1 cut(s) 1022
AccI GTMKAC 2 cut(s) 18, 939
AccIII TCCGGA 2 cut(s) 1303, 2674
AciI CCGC 7 cut(s) 521, 613, 948, 1412, 1495, 2371, 2499
AclI AACGTT 1 cut(s) 2249
AclWI GGATC 6 cut(s) 94, 747, 1022, 1939, 2192, 2447
AcoI YGGCCR 2 cut(s) 1104, 1131
AcsI RAATTY 9 cut(s) 246, 351, 606, 852, 1317, 1546, 1963, 2007, 2490
AcuI CTGAAG 6 cut(s) 983, 996, 1641, 1653, 2684, 2687
AfaI GTAC 5 cut(s) 373, 1227, 1700, 2784, 2799
AfiI CCNNNNNNNGG 6 cut(s) 150, 567, 878, 1022, 1303, 2305
AflIII ACRYGT 1 cut(s) 2779
AhdI GACNNNNNGTC 2 cut(s) 832, 1655
AhlI ACTAGT 1 cut(s) 514
AjnI CCWGG 1 cut(s) 845
AjuI GAANNNNNNNTTGG 4 cut(s) 210, 242, 794, 826
Alw21I GWGCWC 3 cut(s) 528, 1152, 1465
Alw26I GTCTC 4 cut(s) 753, 1727, 2279, 2568
Alw44I GTGCAC 1 cut(s) 1148
AlwI GGATC 6 cut(s) 94, 747, 1022, 1939, 2192, 2447
AlwNI CAGNNNCTG 1 cut(s) 11
Aor13HI TCCGGA 2 cut(s) 1303, 2674
AoxI GGCC 5 cut(s) 336, 843, 949, 1104, 1131
ApaLI GTGCAC 1 cut(s) 1148
ApeKI GCWGC 4 cut(s) 891, 1774, 2086, 2383
ApoI RAATTY 9 cut(s) 246, 351, 606, 852, 1317, 1546, 1963, 2007, 2490
ArsI GACNNNNNNTTYG 2 cut(s) 2546, 2578
AseI ATTAAT 1 cut(s) 1437
Asp700I GAANNNNTTC 3 cut(s) 699, 1548, 2157
Asp718I GGTACC 1 cut(s) 1225
AspLEI GCGC 1 cut(s) 891
AspS9I GGNCC 3 cut(s) 43, 1838, 1993
AsuHPI GGTGA 4 cut(s) 229, 243, 1037, 1090
AvaII GGWCC 3 cut(s) 43, 1838, 1993
AxyI CCTNAGG 1 cut(s) 1230
BaeGI GKGCMC 1 cut(s) 1152
BalI TGGCCA 2 cut(s) 1106, 1133
BanI GGYRCC 1 cut(s) 1225
BanII GRGCYC 3 cut(s) 528, 1465, 2438
BarI GAAGNNNNNNTAC 2 cut(s) 2142, 2174
BauI CACGAG 1 cut(s) 1151
BbsI GAAGAC 3 cut(s) 593, 1651, 2672
Bbv12I GWGCWC 3 cut(s) 528, 1152, 1465
BbvI GCAGC 4 cut(s) 903, 1786, 2073, 2370
BceAI ACGGC 1 cut(s) 400
BcgI CGANNNNNNTGC 2 cut(s) 752, 786
BciT130I CCWGG 1 cut(s) 847
BclI TGATCA 2 cut(s) 619, 2103
BcoDI GTCTC 4 cut(s) 753, 1727, 2279, 2568
BcuI ACTAGT 1 cut(s) 514
BfaI CTAG 4 cut(s) 348, 366, 515, 2625
BfmI CTRYAG 1 cut(s) 393
BfuAI ACCTGC 1 cut(s) 54
BglII AGATCT 1 cut(s) 2155
BisI GCNGC 6 cut(s) 892, 949, 1495, 1775, 2087, 2384
BlsI GCNGC 6 cut(s) 893, 950, 1496, 1776, 2088, 2385
Bme1390I CCNGG 1 cut(s) 847
Bme18I GGWCC 3 cut(s) 43, 1838, 1993
BmeRI GACNNNNNGTC 2 cut(s) 832, 1655
BmgT120I GGNCC 3 cut(s) 43, 1838, 1993
BmiI GGNNCC 6 cut(s) 44, 1227, 1839, 1994, 1995, 2617
BmrFI CCNGG 1 cut(s) 847
BmrI ACTGGG 1 cut(s) 2102
BmsI GCATC 3 cut(s) 111, 130, 202
BmuI ACTGGG 1 cut(s) 2102
BpiI GAAGAC 3 cut(s) 593, 1651, 2672
BplI GAGNNNNNCTC 2 cut(s) 1202, 1234
BpmI CTGGAG 1 cut(s) 1538
Bpu10I CCTNAGC 1 cut(s) 2039
BpuEI CTTGAG 4 cut(s) 2282, 2465, 2506, 2543
BsaJI CCNNGG 5 cut(s) 600, 962, 1322, 2542, 2575
BsaWI WCCGGW 2 cut(s) 1303, 2674
Bsc4I CCNNNNNNNGG 6 cut(s) 150, 567, 878, 1022, 1303, 2305
Bse1I ACTGG 5 cut(s) 1338, 1480, 1639, 2097, 2472
Bse21I CCTNAGG 1 cut(s) 1230
Bse3DI GCAATG 4 cut(s) 131, 622, 1919, 2077
BseAI TCCGGA 2 cut(s) 1303, 2674
BseBI CCWGG 1 cut(s) 847
BseDI CCNNGG 5 cut(s) 600, 962, 1322, 2542, 2575
BseGI GGATG 7 cut(s) 126, 169, 576, 1411, 1579, 1928, 2507
BseLI CCNNNNNNNGG 6 cut(s) 150, 567, 878, 1022, 1303, 2305
BseMI GCAATG 4 cut(s) 131, 622, 1919, 2077
BseMII CTCAG 8 cut(s) 104, 318, 1183, 1221, 1480, 1732, 1738, 2675
BseNI ACTGG 5 cut(s) 1338, 1480, 1639, 2097, 2472
BseRI GAGGAG 3 cut(s) 746, 1339, 2561
BseSI GKGCMC 1 cut(s) 1152
BseXI GCAGC 4 cut(s) 903, 1786, 2073, 2370
Bsh1285I CGRYCG 1 cut(s) 550
BshFI GGCC 5 cut(s) 338, 845, 951, 1106, 1133
BshNI GGYRCC 1 cut(s) 1225
BsiEI CGRYCG 1 cut(s) 550
BsiHKAI GWGCWC 3 cut(s) 528, 1152, 1465
BsiSI CCGG 2 cut(s) 1304, 2675
BslFI GGGAC 3 cut(s) 56, 1851, 2006
BslI CCNNNNNNNGG 6 cut(s) 150, 567, 878, 1022, 1303, 2305
BsmAI GTCTC 4 cut(s) 753, 1727, 2279, 2568
BsmBI CGTCTC 1 cut(s) 753
BsmFI GGGAC 3 cut(s) 56, 1851, 2006
BsmI GAATGC 2 cut(s) 171, 699
BsnI GGCC 5 cut(s) 338, 845, 951, 1106, 1133
Bsp1286I GDGCHC 4 cut(s) 528, 1152, 1465, 2438
Bsp13I TCCGGA 2 cut(s) 1303, 2674
BspACI CCGC 7 cut(s) 521, 613, 948, 1412, 1495, 2371, 2499
BspANI GGCC 5 cut(s) 338, 845, 951, 1106, 1133
BspCNI CTCAG 8 cut(s) 105, 319, 1182, 1222, 1479, 1731, 1737, 2676
BspEI TCCGGA 2 cut(s) 1303, 2674
BspHI TCATGA 3 cut(s) 528, 955, 1252
BspLI GGNNCC 6 cut(s) 44, 1227, 1839, 1994, 1995, 2617
BspMI ACCTGC 1 cut(s) 54
BspPI GGATC 6 cut(s) 94, 747, 1022, 1939, 2192, 2447
BspQI GCTCTTC 1 cut(s) 759
BspT107I GGYRCC 1 cut(s) 1225
BsrDI GCAATG 4 cut(s) 131, 622, 1919, 2077
BsrI ACTGG 5 cut(s) 1338, 1480, 1639, 2097, 2472
BssECI CCNNGG 5 cut(s) 600, 962, 1322, 2542, 2575
BssNAI GTATAC 1 cut(s) 19
BssSI CACGAG 1 cut(s) 1151
BssT1I CCWWGG 2 cut(s) 600, 2542
Bst1107I GTATAC 1 cut(s) 19
Bst2BI CACGAG 1 cut(s) 1151
Bst2UI CCWGG 1 cut(s) 847
Bst4CI ACNGT 8 cut(s) 394, 548, 1147, 1225, 1561, 2002, 2723, 2787
Bst6I CTCTTC 2 cut(s) 759, 975
BstC8I GCNNGC 6 cut(s) 771, 1093, 1097, 1197, 1613, 1750
BstF5I GGATG 7 cut(s) 126, 169, 576, 1411, 1579, 1928, 2507
BstHHI GCGC 1 cut(s) 891
BstMAI GTCTC 4 cut(s) 753, 1727, 2279, 2568
BstMCI CGRYCG 1 cut(s) 550
BstMWI GCNNNNNNNGC 2 cut(s) 212, 344
BstNI CCWGG 1 cut(s) 847
BstNSI RCATGY 3 cut(s) 1615, 2363, 2783
BstSCI CCNGG 1 cut(s) 845
BstSFI CTRYAG 1 cut(s) 393
BstSLI GKGCMC 1 cut(s) 1152
BstV1I GCAGC 4 cut(s) 903, 1786, 2073, 2370
BstV2I GAAGAC 3 cut(s) 593, 1651, 2672
BstX2I RGATCY 3 cut(s) 99, 2155, 2452
BstXI CCANNNNNNTGG 1 cut(s) 488
BstYI RGATCY 3 cut(s) 99, 2155, 2452
BstZ17I GTATAC 1 cut(s) 19
Bsu36I CCTNAGG 1 cut(s) 1230
BsuRI GGCC 5 cut(s) 338, 845, 951, 1106, 1133
BtsCI GGATG 7 cut(s) 126, 169, 576, 1411, 1579, 1928, 2507
BtsIMutI CAGTG 3 cut(s) 1152, 2007, 2465
BveI ACCTGC 1 cut(s) 54
Cac8I GCNNGC 6 cut(s) 771, 1093, 1097, 1197, 1613, 1750
CaiI CAGNNNCTG 1 cut(s) 11
CciI TCATGA 3 cut(s) 528, 955, 1252
CfoI GCGC 1 cut(s) 891
Cfr13I GGNCC 3 cut(s) 43, 1838, 1993
CseI GACGC 1 cut(s) 650
Csp6I GTAC 5 cut(s) 372, 1226, 1699, 2783, 2798
CspCI CAANNNNNGTGG 4 cut(s) 434, 469, 2047, 2082
CviQI GTAC 5 cut(s) 372, 1226, 1699, 2783, 2798
DriI GACNNNNNGTC 2 cut(s) 832, 1655
EaeI YGGCCR 2 cut(s) 1104, 1131
Eam1104I CTCTTC 2 cut(s) 759, 975
Eam1105I GACNNNNNGTC 2 cut(s) 832, 1655
EarI CTCTTC 2 cut(s) 759, 975
EciI GGCGGA 2 cut(s) 536, 2386
Ecl136II GAGCTC 2 cut(s) 526, 1463
Eco130I CCWWGG 2 cut(s) 600, 2542
Eco147I AGGCCT 1 cut(s) 845
Eco24I GRGCYC 3 cut(s) 528, 1465, 2438
Eco32I GATATC 2 cut(s) 1162, 1864
Eco47I GGWCC 3 cut(s) 43, 1838, 1993
Eco53kI GAGCTC 2 cut(s) 526, 1463
Eco57I CTGAAG 6 cut(s) 983, 996, 1641, 1653, 2684, 2687
Eco81I CCTNAGG 1 cut(s) 1230
EcoICRI GAGCTC 2 cut(s) 526, 1463
EcoO109I RGGNCCY 2 cut(s) 43, 1993
EcoRII CCWGG 1 cut(s) 845
EcoRV GATATC 2 cut(s) 1162, 1864
EcoT14I CCWWGG 2 cut(s) 600, 2542
EcoT38I GRGCYC 3 cut(s) 528, 1465, 2438
ErhI CCWWGG 2 cut(s) 600, 2542
Esp3I CGTCTC 1 cut(s) 753
FalI AAGNNNNNCTT 2 cut(s) 268, 300
FaqI GGGAC 3 cut(s) 56, 1851, 2006
FbaI TGATCA 2 cut(s) 619, 2103
FblI GTMKAC 2 cut(s) 18, 939
Fnu4HI GCNGC 6 cut(s) 892, 949, 1495, 1775, 2087, 2384
FokI GGATG 7 cut(s) 133, 176, 583, 1418, 1586, 1915, 2514
FriOI GRGCYC 3 cut(s) 528, 1465, 2438
Fsp4HI GCNGC 6 cut(s) 892, 949, 1495, 1775, 2087, 2384
FspBI CTAG 4 cut(s) 348, 366, 515, 2625
GlaI GCGC 1 cut(s) 890
GluI GCNGC 6 cut(s) 892, 949, 1495, 1775, 2087, 2384
GsuI CTGGAG 1 cut(s) 1538
HaeIII GGCC 5 cut(s) 338, 845, 951, 1106, 1133
HapII CCGG 2 cut(s) 1304, 2675
HgaI GACGC 1 cut(s) 650
HhaI GCGC 1 cut(s) 891
Hin6I GCGC 1 cut(s) 889
HinP1I GCGC 1 cut(s) 889
HincII GTYRAC 2 cut(s) 2169, 2424
HindII GTYRAC 2 cut(s) 2169, 2424
HindIII AAGCTT 1 cut(s) 779
HinfI GANTC 8 cut(s) 8, 105, 115, 1592, 1709, 2264, 2671, 2678
HpaI GTTAAC 1 cut(s) 2424
HpaII CCGG 2 cut(s) 1304, 2675
HphI GGTGA 4 cut(s) 229, 243, 1037, 1090
Hpy166II GTNNAC 8 cut(s) 19, 374, 494, 940, 1150, 2125, 2169, 2424
Hpy8I GTNNAC 8 cut(s) 19, 374, 494, 940, 1150, 2125, 2169, 2424
Hpy99I CGWCG 5 cut(s) 95, 421, 880, 2150, 2346
HpyCH4III ACNGT 8 cut(s) 394, 548, 1147, 1225, 1561, 2002, 2723, 2787
HpyCH4IV ACGT 3 cut(s) 90, 2148, 2249
HpyF10VI GCNNNNNNNGC 2 cut(s) 212, 344
HpySE526I ACGT 3 cut(s) 90, 2148, 2249
HspAI GCGC 1 cut(s) 889
KflI GGGWCCC 1 cut(s) 1993
Kpn2I TCCGGA 2 cut(s) 1303, 2674
KpnI GGTACC 1 cut(s) 1229
Ksp22I TGATCA 2 cut(s) 619, 2103
KspAI GTTAAC 1 cut(s) 2424
LguI GCTCTTC 1 cut(s) 759
LmnI GCTCC 4 cut(s) 523, 1057, 1460, 2615
Lsp1109I GCAGC 4 cut(s) 903, 1786, 2073, 2370
LweI GCATC 3 cut(s) 111, 130, 202
MaeI CTAG 4 cut(s) 348, 366, 515, 2625
MaeII ACGT 3 cut(s) 90, 2148, 2249
MaeIII GTNAC 5 cut(s) 1427, 1514, 1728, 1851, 2317
MfeI CAATTG 1 cut(s) 446
MflI RGATCY 3 cut(s) 99, 2155, 2452
MhlI GDGCHC 4 cut(s) 528, 1152, 1465, 2438
MlsI TGGCCA 2 cut(s) 1106, 1133
MluNI TGGCCA 2 cut(s) 1106, 1133
MlyI GAGTC 4 cut(s) 17, 114, 124, 2273
MmeI TCCRAC 2 cut(s) 98, 1737
Mox20I TGGCCA 2 cut(s) 1106, 1133
MroI TCCGGA 2 cut(s) 1303, 2674
MroXI GAANNNNTTC 3 cut(s) 699, 1548, 2157
MscI TGGCCA 2 cut(s) 1106, 1133
MslI CAYNNNNRTG 1 cut(s) 1112
Msp20I TGGCCA 2 cut(s) 1106, 1133
MspI CCGG 2 cut(s) 1304, 2675
MspR9I CCNGG 1 cut(s) 847
MunI CAATTG 1 cut(s) 446
Mva1269I GAATGC 2 cut(s) 171, 699
MvaI CCWGG 1 cut(s) 847
MwoI GCNNNNNNNGC 2 cut(s) 212, 344
NlaIV GGNNCC 6 cut(s) 44, 1227, 1839, 1994, 1995, 2617
NmeAIII GCCGAG 1 cut(s) 987
NmuCI GTSAC 2 cut(s) 1514, 1728
NspI RCATGY 3 cut(s) 1615, 2363, 2783
PaeI GCATGC 1 cut(s) 1615
PagI TCATGA 3 cut(s) 528, 955, 1252
PceI AGGCCT 1 cut(s) 845
PciI ACATGT 1 cut(s) 2779
PciSI GCTCTTC 1 cut(s) 759
PcsI WCGNNNNNNNCGW 1 cut(s) 425
PctI GAATGC 2 cut(s) 171, 699
PdmI GAANNNNTTC 3 cut(s) 699, 1548, 2157
PfeI GAWTC 4 cut(s) 1592, 1709, 2671, 2678
PflMI CCANNNNNTGG 1 cut(s) 1022
PkrI GCNGC 6 cut(s) 893, 950, 1496, 1776, 2088, 2385
PleI GAGTC 4 cut(s) 16, 113, 123, 2272
PpsI GAGTC 4 cut(s) 16, 113, 123, 2272
PpuMI RGGWCCY 2 cut(s) 43, 1993
PscI ACATGT 1 cut(s) 2779
PshBI ATTAAT 1 cut(s) 1437
Psp124BI GAGCTC 2 cut(s) 528, 1465
Psp1406I AACGTT 1 cut(s) 2249
Psp5II RGGWCCY 2 cut(s) 43, 1993
Psp6I CCWGG 1 cut(s) 845
PspGI CCWGG 1 cut(s) 845
PspN4I GGNNCC 6 cut(s) 44, 1227, 1839, 1994, 1995, 2617
PspPI GGNCC 3 cut(s) 43, 1838, 1993
PspPPI RGGWCCY 2 cut(s) 43, 1993
PstNI CAGNNNCTG 1 cut(s) 11
PsuI RGATCY 3 cut(s) 99, 2155, 2452
RsaI GTAC 5 cut(s) 373, 1227, 1700, 2784, 2799
RsaNI GTAC 5 cut(s) 372, 1226, 1699, 2783, 2798
RseI CAYNNNNRTG 1 cut(s) 1112
SacI GAGCTC 2 cut(s) 528, 1465
SapI GCTCTTC 1 cut(s) 759
SatI GCNGC 6 cut(s) 892, 949, 1495, 1775, 2087, 2384
Sau96I GGNCC 3 cut(s) 43, 1838, 1993
SchI GAGTC 4 cut(s) 17, 114, 124, 2273
ScrFI CCNGG 1 cut(s) 847
SduI GDGCHC 4 cut(s) 528, 1152, 1465, 2438
SfaNI GCATC 3 cut(s) 111, 130, 202
SfcI CTRYAG 1 cut(s) 393
SinI GGWCC 3 cut(s) 43, 1838, 1993
SmiMI CAYNNNNRTG 1 cut(s) 1112
SmlI CTYRAG 4 cut(s) 2261, 2444, 2485, 2558
SmoI CTYRAG 4 cut(s) 2261, 2444, 2485, 2558
SpeI ACTAGT 1 cut(s) 514
SphI GCATGC 1 cut(s) 1615
SseBI AGGCCT 1 cut(s) 845
SsiI CCGC 7 cut(s) 521, 613, 948, 1412, 1495, 2371, 2499
SspI AATATT 2 cut(s) 809, 900
SspMI CTAG 4 cut(s) 348, 366, 515, 2625
SstI GAGCTC 2 cut(s) 528, 1465
StuI AGGCCT 1 cut(s) 845
StyD4I CCNGG 1 cut(s) 845
StyI CCWWGG 2 cut(s) 600, 2542
TaaI ACNGT 8 cut(s) 394, 548, 1147, 1225, 1561, 2002, 2723, 2787
TaiI ACGT 3 cut(s) 93, 2151, 2252
TaqI TCGA 8 cut(s) 93, 108, 419, 550, 725, 836, 1529, 1818
TaqII GACCGA 1 cut(s) 957
TatI WGTACW 2 cut(s) 371, 1698
TauI GCSGC 2 cut(s) 951, 1497
TfiI GAWTC 4 cut(s) 1592, 1709, 2671, 2678
TscAI CASTG 3 cut(s) 1152, 2007, 2472
TseFI GTSAC 2 cut(s) 1514, 1728
TseI GCWGC 4 cut(s) 891, 1774, 2086, 2383
Tsp45I GTSAC 2 cut(s) 1514, 1728
TspDTI ATGAA 8 cut(s) 545, 549, 944, 1392, 1407, 1590, 2283, 2503
TspGWI ACGGA 1 cut(s) 258
TspRI CASTG 3 cut(s) 1152, 2007, 2472
Van91I CCANNNNNTGG 1 cut(s) 1022
VneI GTGCAC 1 cut(s) 1148
VpaK11BI GGWCC 3 cut(s) 43, 1838, 1993
VspI ATTAAT 1 cut(s) 1437
XapI RAATTY 9 cut(s) 246, 351, 606, 852, 1317, 1546, 1963, 2007, 2490
XceI RCATGY 3 cut(s) 1615, 2363, 2783
XcmI CCANNNNNNNNNTGG 2 cut(s) 568, 687
XmiI GTMKAC 2 cut(s) 18, 939
XmnI GAANNNNTTC 3 cut(s) 699, 1548, 2157
XspI CTAG 4 cut(s) 348, 366, 515, 2625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.