Rh6AG136800

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0000069
Physical Location & Seq
Reverse (-)
20815719 .. 20821328
5610 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG136800.1

Sequence Viewer

Length: 2784 bp
ATGGCAGAAATTGTGGTGTCCGCCGTCGCTACAAGCCTTGCTGACCTGCTCAAGGACCTGCTTCTGGAGGAATCTAAATCCGTACTAAGGGTGAGGAAAAACGTTGGGGATATCCAAAAAGAATTGATGCGGATAAAATGTTTCTTGAAAGATGCGGAAGAGAAGCAAGAGCAAGATAGTAGGCTTCAAAATTGGGTTTCAGACATACGAGAAATCGCTTATGATGCTGAGGATGTCATTCGAATGTTTTCCATCAGAGTTGCAATGGAAATCTCAAATCCGGTCAAATGGTATGCCTGGCTGAATCTCGGCCAACTTCGACACCGTTGCAAAGTTGGGTCTGAGATTAAGGCCATACAAGCTGAAATCTCTAGGCTTCGCATATCTTCCCAAACCTATAACTTGATTCCTGTTGGACAAGGGCCATCCAGCAATGCTCTTGATGATCGGCGGCAATATTCCCATGAAAATGAGGATATTGTTGGGTTCGAAAGTAGCATAAAAGAAATAGTGGCAGATCTTGTGGATGATGATCGCCCTGGTTGGGTTGTGTCAATTTGTGGGATGGCAGGTATTGGAAAGACAACTCTGGCCAGGGAACTTTACCGTAATGATCGAATTAGGCGCCATTTTGATGGTTTCGGATGGGCTTGTATATCTCAGCAATTTAAACCAGAAGATGCTTGGGAACAAGTTTTGTTCAATCTCGTCACTCCATCCAAAGAGGAGAGGCAAAATATTAGAGAGCTGAGACACGATGAGCTTGTAAAAAAGCTTTATCAGGTTCAGATAGAGAAGAAATGTTTGGTGATCCTTGATGACATTTGGAAGGCCGAGGACTGGAACAAGCTACGTCATGCTTTCCCAACTGCCGGAAAAGGGTGCAGCAGGGTATTGGTTACAACTCGTTTGAAGAATATTGTAGTTCCTGCTGACAGTGGAGGTCGTCCTCCTCATGAACTAAAATTTTTATCTGTAGAAGACGGATGGGAGTTACTTCGGAGGAAGGCATTTCCAGAGGCGAAGAATAATCCAGACTTTACTGTTGGCAAAGTTAAGGAGAATTTAGGGAAGGAAATAGTCGAGAAATGTGATGGCTTACCATTGGCTATTGTTGTACTCGGAGGACTCTTAGCAACTAAAGAATCAGAACACGATTGGGAGAGGATTAAAGATATGCCGTCTTTTTTACAGCAAGGCAACGATCACAATAATATACTACCAAAGGTTTTGGCTCTAAGCTATAATGAGCTGCCACACGAATTGAAACCTTGTTTCCTTTATTTGAGTCATTTCCCAGAGGATGCTGCAATACCTAAAAAGAAATTGATTCAATTATGGATTGCAGAGGGCATTGTATCACATAATTCAGAACAAGGTGAACAGGAGACAGATGAAGTGCTAGAGGATGTGGCAGAAGGCCACTTGATATCCTTGATCAATAGGTGCATGGTTCAAGTGGCAGAATTTGGGTCTACTGGAAGAATCAAAACGTGCCGCCTCCATGATATTATGCGTGATTTTTGCTTGTTAAGAGCAAAGAAGGAAAACTTTCTCAAGCTTGTTCGCTATTCAAATGGAAGTGATGCTGATTCTTCTTTAAGCCTGCCATCAGGTAAAACGCGAAGACTTGCTATCTACTTGCCTGAAGACGTTGATAGATTTATCTCATCGCCACGCAAACAATATTCCAACCTCAGGTCACTTTTGTATTTCCATGGGAAAAGGTGTAGAGTGGAGGATTGGAACCTCATTAAAAAGATTTTGAACCTCAAAATGCTCAGAGTCCTAGATCTTGAAGGGGTAAATGGACCTTATGGAAAGTTGCCAAAAGAGACTGGGGACCTCATTCAGCTGCAGTTTCTAAGTTTAAAGAAAACATATATCGCAGTTCTTCCACCATCCATAGGTAATTTGATCTTCTTGAAAACCCTAAATCTGCAGACTATTAGTAAATTTAGTAGGGATTCAACAGTACAAATTCCAAATGTGATATGGAAGATGAAGAGGTTGACCCATTTATATCTTCCCAAGTGGTGTGGCAACATGGTTAATGAGCTGAAGTTGTCCAATCTTCACCGCTTGCAGACACTAGTGAACTTCCCTGCAAACAAATGTGATGTACAAGATCTTCTGAAATTGACTAAACTGAGGAAACTGGTGCTCAATGACCCAGCACATTTCAAAAGTTTGGTAGAAATTTTCAGTCCCCCAAATGATGTCACATTAAGCTGCCTTCAGTCCTTGTCCTTTAAAAGTGACACACTTGCATTCCCCAATGAGGTAGTAGATGTCAGGCAACTCCTGTCAAGCTGTTCAGCTCTTCAGAAGTTTCATGTGGAAGGACGAATAGCAAAGCTACCACAATACCTTCAGTTCCCTCCAAGCATTGCCAAGTTAACTTTATGGGGTTCTAATCTTGTGGAAGATCCAATCCCAACTTTGGAGAAGCTGCCTAACTTGAGAATTCTGAGCGGATGGCAAATGTTCATGGGGAAGAAAATGGTTTTCTCCTATCAAGGTTTTCCTAAACTCAGAAGTCTACTGCTTCGTGGCTTGATGCATTTTGAGGACTGGATGATGGAGGAAGGAGCTATGCCGAGTCTTTATCGATTGGAAATCTCAAATTGCATTAAACTGAAGACAATTCCTGCTGCAGTAAGTTCTGTCAAAACTCTTCAGGAATTGGAGATATGCGGGTGCCTATTCAAGATCAAAATTAGACCTGAAGAAGAGGACTTTTACAAAGTCCGACATGTGCCCTCCGTTGTGATTCGGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

927

Amino Acids

106.14

Weight (kDa)

8.8

Isoelectric Point (pI)

46.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 11 - 89 1.2e-21 Rx N-terminal domain
NB-ARC PF00931 164 - 340 3e-38 NB-ARC domain
WHD_DRP PF23559 432 - 508 3.5e-16 Disease resistance protein Winged helix domain
LRR_14 PF23598 583 - 882 6.6e-34 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000069)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10920 AT1G10920 AT1G10920 AT1G10920 AT1G10920 AT1G50180 AT1G53350 AT1G58390 AT1G58390 AT1G58400 AT1G58400 AT1G58400 AT1G58410 AT1G58410 AT1G58410 AT1G58410 AT1G58602 AT1G58602 AT1G58807 AT1G58807 AT1G58848 AT1G58848 AT1G59124 AT1G59124 AT1G59218 AT1G59218 AT1G59218 AT1G59620 AT1G59780 AT1G59780 AT5G35450 AT5G35450 AT5G43470 AT5G43470 AT5G43470 AT5G43470 AT5G48620 AT5G48620 AT5G48620 AT5G48620 AT5G48620 AT5G48620
fragaria_vesca FvH4_4g04072 FvH4_4g22931 FvH4_4g22931 FvH4_4g22940 FvH4_6g49940 FvH4_6g49940 FvH4_7g08100 FvH4_7g08870 FvH4_7g08870 FvH4_7g09280 FvH4_7g09290 FvH4_7g15190 FvH4_7g15200 FvH4_7g15240 FvH4_7g15240 FvH4_7g33440
malus_domestica MD00G1174600.v1.1 MD01G1113700.v1.1 MD03G1071300.v1.1 MD04G1041500.v1.1 MD04G1238000.v1.1 MD07G1029000.v1.1 MD07G1092100.v1.1 MD07G1092300.v1.1 MD07G1092600.v1.1 MD12G1256300.v1.1 MD13G1128200.v1.1 MD13G1128400.v1.1 MD17G1268000.v1.1
prunus_persica Prupe.1G041300_v2.0.a1 Prupe.1G041300_v2.0.a1 Prupe.1G042900_v2.0.a1 Prupe.1G043100_v2.0.a1 Prupe.1G043100_v2.0.a1 Prupe.1G043100_v2.0.a1 Prupe.1G043100_v2.0.a1 Prupe.1G043600_v2.0.a1 Prupe.1G043700_v2.0.a1 Prupe.1G043700_v2.0.a1 Prupe.1G043700_v2.0.a1 Prupe.1G043700_v2.0.a1 Prupe.1G043700_v2.0.a1 Prupe.1G043700_v2.0.a1 Prupe.1G046700_v2.0.a1 Prupe.1G047700_v2.0.a1 Prupe.1G047900_v2.0.a1 Prupe.1G048000_v2.0.a1 Prupe.1G048500_v2.0.a1 Prupe.1G048500_v2.0.a1 Prupe.1G049900_v2.0.a1 Prupe.1G051500_v2.0.a1 Prupe.1G051800_v2.0.a1 Prupe.1G051900_v2.0.a1 Prupe.1G052200_v2.0.a1 Prupe.1G194900_v2.0.a1 Prupe.1G217800_v2.0.a1 Prupe.1G217800_v2.0.a1 Prupe.1G217800_v2.0.a1 Prupe.1G217800_v2.0.a1 Prupe.1G217800_v2.0.a1 Prupe.1G217800_v2.0.a1 Prupe.1G217800_v2.0.a1 Prupe.1G217800_v2.0.a1 Prupe.1G217800_v2.0.a1 Prupe.1G217900_v2.0.a1 Prupe.1G217900_v2.0.a1 Prupe.1G217900_v2.0.a1 Prupe.1G217900_v2.0.a1 Prupe.1G217900_v2.0.a1 Prupe.2G117200_v2.0.a1 Prupe.2G117200_v2.0.a1 Prupe.2G117200_v2.0.a1 Prupe.2G117200_v2.0.a1 Prupe.2G117300_v2.0.a1 Prupe.2G117400_v2.0.a1 Prupe.2G117500_v2.0.a1 Prupe.2G117700_v2.0.a1 Prupe.2G118000_v2.0.a1 Prupe.2G118000_v2.0.a1 Prupe.2G118000_v2.0.a1 Prupe.2G118000_v2.0.a1 Prupe.2G118000_v2.0.a1 Prupe.2G118000_v2.0.a1 Prupe.2G118000_v2.0.a1 Prupe.2G119900_v2.0.a1
pyrus_communis pycom03g05750 pycom04g21110 pycom07g07530 pycom07g07590 pycom13g11110 pycom13g11140 pycom13g11160 pycom13g11170 pycom17g26680
rosa_chinensis RchiOBHm_Chr1g0341231 RchiOBHm_Chr1g0344091 RchiOBHm_Chr1g0344161 RchiOBHm_Chr1g0345001 RchiOBHm_Chr1g0345351 RchiOBHm_Chr1g0345371 RchiOBHm_Chr1g0345391 RchiOBHm_Chr1g0345531 RchiOBHm_Chr1g0345551 RchiOBHm_Chr1g0345571 RchiOBHm_Chr1g0345581 RchiOBHm_Chr1g0345661 RchiOBHm_Chr1g0345691 RchiOBHm_Chr1g0345761 RchiOBHm_Chr1g0345791 RchiOBHm_Chr1g0345821 RchiOBHm_Chr1g0345911 RchiOBHm_Chr1g0345941 RchiOBHm_Chr1g0357611 RchiOBHm_Chr1g0357911 RchiOBHm_Chr1g0357921 RchiOBHm_Chr1g0357931 RchiOBHm_Chr1g0357971 RchiOBHm_Chr1g0358121 RchiOBHm_Chr1g0383491 RchiOBHm_Chr1g0383511 RchiOBHm_Chr1g0383521 RchiOBHm_Chr3g0480581 RchiOBHm_Chr4g0394341 RchiOBHm_Chr4g0394361 RchiOBHm_Chr4g0394411 RchiOBHm_Chr4g0394441 RchiOBHm_Chr4g0407681 RchiOBHm_Chr4g0429191 RchiOBHm_Chr4g0429201 RchiOBHm_Chr4g0429211 RchiOBHm_Chr4g0429221 RchiOBHm_Chr6g0265091
rosa_laevigata RLG00000007088 RLG00000008700 RLG00000009190 RLG00000009702 RLG00000014210 RLG00000028829 RLG00000028833 RLG00000028837 RLG00000028838 RLG00000028921 RLG00000028926
rosa_multiflora Rmu_co8366955.1_g000001 Rmu_co8394205.1_g000001 Rmu_sc0000019.1_g000001 Rmu_sc0000054.1_g000031 Rmu_sc0000054.1_g000033 Rmu_sc0000200.1_g000002 Rmu_sc0000200.1_g000007 Rmu_sc0000200.1_g000018 Rmu_sc0000200.1_g000020 Rmu_sc0000200.1_g000021 Rmu_sc0000251.1_g000012 Rmu_sc0000251.1_g000015 Rmu_sc0000251.1_g000028 Rmu_sc0000253.1_g000015 Rmu_sc0000255.1_g000045 Rmu_sc0000255.1_g000046 Rmu_sc0001292.1_g000013 Rmu_sc0001292.1_g000018 Rmu_sc0001292.1_g000028 Rmu_sc0001292.1_g000029 Rmu_sc0001349.1_g000006 Rmu_sc0001349.1_g000014 Rmu_sc0001582.1_g000006 Rmu_sc0001582.1_g000018 Rmu_sc0001582.1_g000020 Rmu_sc0001692.1_g000007 Rmu_sc0001692.1_g000024 Rmu_sc0001692.1_g000026 Rmu_sc0001692.1_g000029 Rmu_sc0001692.1_g000030 Rmu_sc0001835.1_g000006 Rmu_sc0001835.1_g000010 Rmu_sc0002071.1_g000005 Rmu_sc0002276.1_g000002 Rmu_sc0002276.1_g000009 Rmu_sc0002329.1_g000018 Rmu_sc0002329.1_g000023 Rmu_sc0002329.1_g000025 Rmu_sc0002523.1_g000005 Rmu_sc0003573.1_g000005 Rmu_sc0003573.1_g000006 Rmu_sc0004099.1_g000021 Rmu_sc0005812.1_g000009 Rmu_sc0006271.1_g000002 Rmu_sc0006287.1_g000038 Rmu_sc0006439.1_g000008 Rmu_sc0007456.1_g000004 Rmu_sc0007581.1_g000009 Rmu_sc0007849.1_g000014 Rmu_sc0008115.1_g000012 Rmu_sc0010521.1_g000004 Rmu_sc0012471.1_g000017 Rmu_sc0014762.1_g000001 Rmu_sc0015150.1_g000003 Rmu_sc0017221.1_g000001 Rmu_sc0017299.1_g000001 Rmu_sc0017299.1_g000002 Rmu_sc0021457.1_g000001 Rmu_sc0022067.1_g000001 Rmu_sc0033275.1_g000001 Rmu_ssc0000245.1_g000012 Rmu_ssc0000359.1_g000035 Rmu_ssc0000359.1_g000067 Rmu_ssc0000359.1_g000072 Rmu_ssc0000434.1_g000005 Rmu_ssc0000434.1_g000007
rosa_roxburghii Rroxscaffold_4G00298420 Rroxscaffold_4G00298720 Rroxscaffold_4G00308990 Rroxscaffold_4G00309000 Rroxscaffold_4G00309010 Rroxscaffold_4G00309050 Rroxscaffold_4G00309070 Rroxscaffold_4G00309100 Rroxscaffold_4G00310030 Rroxscaffold_4G00310050 Rroxscaffold_4G00311880 Rroxscaffold_5G00339420 Rroxscaffold_5G00339450 Rroxscaffold_5G00344880 Rroxscaffold_5G00352270 Rroxscaffold_5G00362240 Rroxscaffold_5G00370840 Rroxscaffold_7G00202970
rosa_rugosa Rorug01G0159100.1 Rorug01G0159200.1 Rorug01G0171700 Rorug01G0171900 Rorug01G0172000 Rorug01G0191700 Rorug01G0191800 Rorug01G0256900 Rorug01G0257000 Rorug01G0257100 Rorug03G0198000 Rorug03G0366300.1 Rorug03G0366400.1 Rorug03G0366500.1 Rorug04G0081200 Rorug04G0081200 Rorug04G0081200 Rorug04G0233700 Rorug04G0233800 Rorug04G0233800 Rorug06G0013300
rosa_samantha Rh1CG138800 Rh1CG161300 Rh1CG173800 Rh1CG179800 Rh1CG180100 Rh1CG180500 Rh1CG180800 Rh1CG180900 Rh1CG194100 Rh1DG205100 Rh3AG236300 Rh4AG052000 Rh4AG052100 Rh4AG145900 Rh4AG289700 Rh4AG289800 Rh6AG136800 Rh6AG137300 Rh6BG133600 Rh6CG132000 Rh6CG134000 Rh6DG119300
rosa_wichuraiana Rw0G003420 Rw1G015500 Rw1G016110 Rw1G016180 Rw1G016210 Rw1G016230 Rw1G016280 Rw1G016440 Rw1G016450 Rw1G016460 Rw1G016480 Rw1G017490 Rw1G024140 Rw1G024160 Rw3G022530 Rw4G004140 Rw4G004190 Rw4G011850 Rw6G011800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 54, 66, 560
AccB1I GGYRCC 2 cut(s) 624, 2700
AccBSI CCGCTC 1 cut(s) 2475
AccI GTMKAC 2 cut(s) 1475, 2542
AccII CGCG 1 cut(s) 1624
AciI CCGC 8 cut(s) 21, 130, 155, 451, 1498, 2080, 2475, 2697
AclI AACGTT 1 cut(s) 102
AclWI GGATC 2 cut(s) 805, 2423
AcoI YGGCCR 2 cut(s) 310, 591
AcsI RAATTY 7 cut(s) 965, 1063, 1466, 1955, 1980, 2199, 2466
AcuI CTGAAG 8 cut(s) 1668, 2081, 2222, 2309, 2357, 2660, 2663, 2748
AcyI GRCGYC 1 cut(s) 625
AfaI GTAC 4 cut(s) 84, 1119, 1977, 2124
AflIII ACRYGT 1 cut(s) 2755
AhlI ACTAGT 1 cut(s) 2092
AjnI CCWGG 3 cut(s) 296, 538, 593
AjuI GAANNNNNNNTTGG 2 cut(s) 788, 820
Alw21I GWGCWC 1 cut(s) 2166
Alw26I GTCTC 3 cut(s) 745, 1382, 1829
AlwI GGATC 2 cut(s) 805, 2423
AoxI GGCC 6 cut(s) 310, 351, 422, 591, 831, 1420
ApeKI GCWGC 7 cut(s) 885, 1252, 1307, 1855, 2232, 2452, 2654
ApoI RAATTY 7 cut(s) 965, 1063, 1466, 1955, 1980, 2199, 2466
Asp700I GAANNNNTTC 2 cut(s) 247, 1551
AspLEI GCGC 1 cut(s) 627
AspS9I GGNCC 4 cut(s) 55, 422, 1811, 1843
AsuHPI GGTGA 4 cut(s) 103, 820, 1391, 2069
AsuII TTCGAA 2 cut(s) 241, 489
AvaII GGWCC 3 cut(s) 55, 1811, 1843
AxyI CCTNAGG 1 cut(s) 1697
BaeGI GKGCMC 1 cut(s) 2763
BalI TGGCCA 1 cut(s) 593
BanI GGYRCC 2 cut(s) 624, 2700
BarI GAAGNNNNNNTAC 2 cut(s) 2115, 2147
BbsI GAAGAC 4 cut(s) 987, 1633, 1656, 2648
Bbv12I GWGCWC 1 cut(s) 2166
BbvCI CCTCAGC 1 cut(s) 228
BbvI GCAGC 7 cut(s) 897, 1239, 1294, 1842, 2219, 2439, 2641
BceAI ACGGC 2 cut(s) 8, 1165
BcgI CGANNNNNNTGC 2 cut(s) 309, 343
BciT130I CCWGG 3 cut(s) 298, 540, 595
BclI TGATCA 1 cut(s) 1437
BcoDI GTCTC 3 cut(s) 745, 1382, 1829
BcuI ACTAGT 1 cut(s) 2092
BfaI CTAG 4 cut(s) 372, 1403, 1790, 2093
BfmI CTRYAG 4 cut(s) 975, 1856, 1940, 2655
BfoI RGCGCY 1 cut(s) 628
BfuAI ACCTGC 3 cut(s) 54, 66, 560
BglII AGATCT 3 cut(s) 517, 1792, 2128
BisI GCNGC 9 cut(s) 452, 886, 1253, 1308, 1498, 1856, 2233, 2453, 2655
BlsI GCNGC 9 cut(s) 453, 887, 1254, 1309, 1499, 1857, 2234, 2454, 2656
Bme1390I CCNGG 3 cut(s) 298, 540, 595
Bme18I GGWCC 3 cut(s) 55, 1811, 1843
BmgT120I GGNCC 4 cut(s) 55, 422, 1811, 1843
BmiI GGNNCC 4 cut(s) 626, 1748, 1844, 2702
BmrFI CCNGG 3 cut(s) 298, 540, 595
BmrI ACTGGG 1 cut(s) 1848
BmsI GCATC 7 cut(s) 117, 142, 214, 670, 1294, 1576, 2550
BmuI ACTGGG 1 cut(s) 1848
BpiI GAAGAC 4 cut(s) 987, 1633, 1656, 2648
BpmI CTGGAG 1 cut(s) 86
Bpu10I CCTNAGC 1 cut(s) 228
Bpu14I TTCGAA 2 cut(s) 241, 489
BpuEI CTTGAG 3 cut(s) 35, 1541, 2482
Bsa29I ATCGAT 1 cut(s) 2611
BsaBI GATNNNNATC 1 cut(s) 531
BsaHI GRCGYC 1 cut(s) 625
BsaJI CCNNGG 4 cut(s) 538, 594, 834, 1717
BsaWI WCCGGW 1 cut(s) 280
Bse1I ACTGG 5 cut(s) 845, 1483, 1843, 2163, 2579
Bse21I CCTNAGG 1 cut(s) 1697
Bse3DI GCAATG 3 cut(s) 270, 439, 2388
Bse8I GATNNNNATC 1 cut(s) 531
BseBI CCWGG 3 cut(s) 298, 540, 595
BseCI ATCGAT 1 cut(s) 2611
BseDI CCNNGG 4 cut(s) 538, 594, 834, 1717
BseJI GATNNNNATC 1 cut(s) 531
BseMI GCAATG 3 cut(s) 270, 439, 2388
BseMII CTCAG 9 cut(s) 219, 333, 674, 740, 1711, 1795, 2141, 2462, 2548
BseNI ACTGG 5 cut(s) 845, 1483, 1843, 2163, 2579
BseRI GAGGAG 2 cut(s) 740, 942
BseSI GKGCMC 1 cut(s) 2763
BseXI GCAGC 7 cut(s) 897, 1239, 1294, 1842, 2219, 2439, 2641
BseYI CCCAGC 1 cut(s) 2173
BsgI GTGCAG 1 cut(s) 904
Bsh1236I CGCG 1 cut(s) 1624
BshFI GGCC 6 cut(s) 312, 353, 424, 593, 833, 1422
BshNI GGYRCC 2 cut(s) 624, 2700
BshVI ATCGAT 1 cut(s) 2611
BsiHKAI GWGCWC 1 cut(s) 2166
BsiSI CCGG 2 cut(s) 281, 873
BslFI GGGAC 2 cut(s) 1856, 2193
BsmAI GTCTC 3 cut(s) 745, 1382, 1829
BsmFI GGGAC 2 cut(s) 1856, 2193
BsmI GAATGC 1 cut(s) 2270
BsnI GGCC 6 cut(s) 312, 353, 424, 593, 833, 1422
Bsp119I TTCGAA 2 cut(s) 241, 489
Bsp1286I GDGCHC 2 cut(s) 2166, 2763
Bsp1407I TGTACA 1 cut(s) 2122
Bsp19I CCATGG 1 cut(s) 1717
BspACI CCGC 8 cut(s) 21, 130, 155, 451, 1498, 2080, 2475, 2697
BspANI GGCC 6 cut(s) 312, 353, 424, 593, 833, 1422
BspCNI CTCAG 9 cut(s) 220, 334, 673, 741, 1710, 1794, 2142, 2463, 2547
BspDI ATCGAT 1 cut(s) 2611
BspFNI CGCG 1 cut(s) 1624
BspHI TCATGA 1 cut(s) 955
BspLI GGNNCC 4 cut(s) 626, 1748, 1844, 2702
BspMAI CTGCAG 3 cut(s) 1860, 1944, 2659
BspMI ACCTGC 3 cut(s) 54, 66, 560
BspPI GGATC 2 cut(s) 805, 2423
BspQI GCTCTTC 1 cut(s) 2328
BspT104I TTCGAA 2 cut(s) 241, 489
BspT107I GGYRCC 2 cut(s) 624, 2700
BsrBI CCGCTC 1 cut(s) 2475
BsrDI GCAATG 3 cut(s) 270, 439, 2388
BsrGI TGTACA 1 cut(s) 2122
BsrI ACTGG 5 cut(s) 845, 1483, 1843, 2163, 2579
BssECI CCNNGG 4 cut(s) 538, 594, 834, 1717
BssNI GRCGYC 1 cut(s) 625
BssT1I CCWWGG 1 cut(s) 1717
Bst2UI CCWGG 3 cut(s) 298, 540, 595
Bst4CI ACNGT 5 cut(s) 326, 608, 938, 1045, 1975
Bst6I CTCTTC 5 cut(s) 153, 2000, 2328, 2682, 2727
BstACI GRCGYC 1 cut(s) 625
BstAUI TGTACA 1 cut(s) 2122
BstBI TTCGAA 2 cut(s) 241, 489
BstC8I GCNNGC 2 cut(s) 1607, 2084
BstDSI CCRYGG 1 cut(s) 1717
BstENI CCTNNNNNAGG 1 cut(s) 50
BstFNI CGCG 1 cut(s) 1624
BstH2I RGCGCY 1 cut(s) 628
BstHHI GCGC 1 cut(s) 627
BstMAI GTCTC 3 cut(s) 745, 1382, 1829
BstMWI GCNNNNNNNGC 2 cut(s) 224, 359
BstNI CCWGG 3 cut(s) 298, 540, 595
BstNSI RCATGY 1 cut(s) 2759
BstSCI CCNGG 3 cut(s) 296, 538, 593
BstSFI CTRYAG 4 cut(s) 975, 1856, 1940, 2655
BstSLI GKGCMC 1 cut(s) 2763
BstUI CGCG 1 cut(s) 1624
BstV1I GCAGC 7 cut(s) 897, 1239, 1294, 1842, 2219, 2439, 2641
BstV2I GAAGAC 4 cut(s) 987, 1633, 1656, 2648
BstX2I RGATCY 4 cut(s) 517, 1792, 2128, 2428
BstXI CCANNNNNNTGG 1 cut(s) 635
BstYI RGATCY 4 cut(s) 517, 1792, 2128, 2428
Bsu15I ATCGAT 1 cut(s) 2611
Bsu36I CCTNAGG 1 cut(s) 1697
BsuRI GGCC 6 cut(s) 312, 353, 424, 593, 833, 1422
BsuTUI ATCGAT 1 cut(s) 2611
BtgI CCRYGG 1 cut(s) 1717
BtgZI GCGATG 1 cut(s) 1656
BtsIMutI CAGTG 1 cut(s) 943
BveI ACCTGC 3 cut(s) 54, 66, 560
Cac8I GCNNGC 2 cut(s) 1607, 2084
CciI TCATGA 1 cut(s) 955
CfoI GCGC 1 cut(s) 627
Cfr13I GGNCC 4 cut(s) 55, 422, 1811, 1843
ClaI ATCGAT 1 cut(s) 2611
Csp6I GTAC 4 cut(s) 83, 1118, 1976, 2123
CspCI CAANNNNNGTGG 4 cut(s) 1245, 1280, 2020, 2055
CviQI GTAC 4 cut(s) 83, 1118, 1976, 2123
DinI GGCGCC 1 cut(s) 626
DraI TTTAAA 3 cut(s) 670, 1872, 2254
EaeI YGGCCR 2 cut(s) 310, 591
Eam1104I CTCTTC 5 cut(s) 153, 2000, 2328, 2682, 2727
EarI CTCTTC 5 cut(s) 153, 2000, 2328, 2682, 2727
EciI GGCGGA 1 cut(s) 10
Eco130I CCWWGG 1 cut(s) 1717
Eco32I GATATC 2 cut(s) 112, 1431
Eco47I GGWCC 3 cut(s) 55, 1811, 1843
Eco57I CTGAAG 8 cut(s) 1668, 2081, 2222, 2309, 2357, 2660, 2663, 2748
Eco81I CCTNAGG 1 cut(s) 1697
EcoNI CCTNNNNNAGG 1 cut(s) 50
EcoO109I RGGNCCY 2 cut(s) 55, 1843
EcoRI GAATTC 1 cut(s) 2466
EcoRII CCWGG 3 cut(s) 296, 538, 593
EcoRV GATATC 2 cut(s) 112, 1431
EcoT14I CCWWGG 1 cut(s) 1717
EcoT22I ATGCAT 1 cut(s) 2565
EgeI GGCGCC 1 cut(s) 626
EheI GGCGCC 1 cut(s) 626
ErhI CCWWGG 1 cut(s) 1717
FalI AAGNNNNNCTT 2 cut(s) 1535, 1567
FaqI GGGAC 2 cut(s) 1856, 2193
FauI CCCGC 1 cut(s) 2690
FbaI TGATCA 1 cut(s) 1437
FblI GTMKAC 2 cut(s) 1475, 2542
Fnu4HI GCNGC 9 cut(s) 452, 886, 1253, 1308, 1498, 1856, 2233, 2453, 2655
Fsp4HI GCNGC 9 cut(s) 452, 886, 1253, 1308, 1498, 1856, 2233, 2453, 2655
FspBI CTAG 4 cut(s) 372, 1403, 1790, 2093
GlaI GCGC 1 cut(s) 626
GluI GCNGC 9 cut(s) 452, 886, 1253, 1308, 1498, 1856, 2233, 2453, 2655
GsaI CCCAGC 1 cut(s) 2177
GsuI CTGGAG 1 cut(s) 86
HaeII RGCGCY 1 cut(s) 628
HaeIII GGCC 6 cut(s) 312, 353, 424, 593, 833, 1422
HapII CCGG 2 cut(s) 281, 873
HhaI GCGC 1 cut(s) 627
Hin1I GRCGYC 1 cut(s) 625
Hin6I GCGC 1 cut(s) 625
HinP1I GCGC 1 cut(s) 625
HincII GTYRAC 2 cut(s) 2013, 2400
HindII GTYRAC 2 cut(s) 2013, 2400
HindIII AAGCTT 2 cut(s) 773, 1559
HpaI GTTAAC 1 cut(s) 2400
HpaII CCGG 2 cut(s) 281, 873
HphI GGTGA 4 cut(s) 103, 820, 1391, 2069
Hpy166II GTNNAC 6 cut(s) 1382, 1476, 2013, 2098, 2400, 2543
Hpy8I GTNNAC 6 cut(s) 1382, 1476, 2013, 2098, 2400, 2543
Hpy99I CGWCG 1 cut(s) 29
HpyCH4III ACNGT 5 cut(s) 326, 608, 938, 1045, 1975
HpyCH4IV ACGT 4 cut(s) 102, 853, 1493, 1653
HpyF10VI GCNNNNNNNGC 2 cut(s) 224, 359
HpySE526I ACGT 4 cut(s) 102, 853, 1493, 1653
Hsp92I GRCGYC 1 cut(s) 625
HspAI GCGC 1 cut(s) 625
KasI GGCGCC 1 cut(s) 624
Ksp22I TGATCA 1 cut(s) 1437
KspAI GTTAAC 1 cut(s) 2400
LguI GCTCTTC 1 cut(s) 2328
LmnI GCTCC 1 cut(s) 2591
Lsp1109I GCAGC 7 cut(s) 897, 1239, 1294, 1842, 2219, 2439, 2641
LweI GCATC 7 cut(s) 117, 142, 214, 670, 1294, 1576, 2550
MaeI CTAG 4 cut(s) 372, 1403, 1790, 2093
MaeII ACGT 4 cut(s) 102, 853, 1493, 1653
MaeIII GTNAC 6 cut(s) 709, 898, 993, 1701, 2221, 2258
MbiI CCGCTC 1 cut(s) 2475
MflI RGATCY 4 cut(s) 517, 1792, 2128, 2428
MhlI GDGCHC 2 cut(s) 2166, 2763
MlsI TGGCCA 1 cut(s) 593
MluNI TGGCCA 1 cut(s) 593
Mly113I GGCGCC 1 cut(s) 625
MlyI GAGTC 4 cut(s) 1122, 1297, 1794, 2611
MmeI TCCRAC 3 cut(s) 394, 1716, 2776
Mox20I TGGCCA 1 cut(s) 593
Mph1103I ATGCAT 1 cut(s) 2565
MroXI GAANNNNTTC 2 cut(s) 247, 1551
MscI TGGCCA 1 cut(s) 593
MslI CAYNNNNRTG 3 cut(s) 242, 468, 633
Msp20I TGGCCA 1 cut(s) 593
MspA1I CMGCKG 1 cut(s) 1855
MspI CCGG 2 cut(s) 281, 873
MspR9I CCNGG 3 cut(s) 298, 540, 595
Mva1269I GAATGC 1 cut(s) 2270
MvaI CCWGG 3 cut(s) 298, 540, 595
MvnI CGCG 1 cut(s) 1624
MwoI GCNNNNNNNGC 2 cut(s) 224, 359
NarI GGCGCC 1 cut(s) 625
NcoI CCATGG 1 cut(s) 1717
NlaIV GGNNCC 4 cut(s) 626, 1748, 1844, 2702
NmeAIII GCCGAG 3 cut(s) 288, 859, 2625
NmuCI GTSAC 4 cut(s) 709, 1701, 2221, 2258
NsiI ATGCAT 1 cut(s) 2565
NspI RCATGY 1 cut(s) 2759
NspV TTCGAA 2 cut(s) 241, 489
PagI TCATGA 1 cut(s) 955
PciI ACATGT 1 cut(s) 2755
PciSI GCTCTTC 1 cut(s) 2328
PctI GAATGC 1 cut(s) 2270
PdmI GAANNNNTTC 2 cut(s) 247, 1551
PfeI GAWTC 9 cut(s) 71, 304, 406, 1145, 1330, 1485, 1592, 1967, 2773
PkrI GCNGC 9 cut(s) 453, 887, 1254, 1309, 1499, 1857, 2234, 2454, 2656
PleI GAGTC 4 cut(s) 1122, 1296, 1793, 2610
PluTI GGCGCC 1 cut(s) 628
PpsI GAGTC 4 cut(s) 1122, 1296, 1793, 2610
PpuMI RGGWCCY 2 cut(s) 55, 1843
PscI ACATGT 1 cut(s) 2755
Psp1406I AACGTT 1 cut(s) 102
Psp5II RGGWCCY 2 cut(s) 55, 1843
Psp6I CCWGG 3 cut(s) 296, 538, 593
PspFI CCCAGC 1 cut(s) 2173
PspGI CCWGG 3 cut(s) 296, 538, 593
PspN4I GGNNCC 4 cut(s) 626, 1748, 1844, 2702
PspPI GGNCC 4 cut(s) 55, 422, 1811, 1843
PspPPI RGGWCCY 2 cut(s) 55, 1843
PstI CTGCAG 3 cut(s) 1860, 1944, 2659
PsuI RGATCY 4 cut(s) 517, 1792, 2128, 2428
PvuII CAGCTG 1 cut(s) 1855
RsaI GTAC 4 cut(s) 84, 1119, 1977, 2124
RsaNI GTAC 4 cut(s) 83, 1118, 1976, 2123
RseI CAYNNNNRTG 3 cut(s) 242, 468, 633
SapI GCTCTTC 1 cut(s) 2328
SatI GCNGC 9 cut(s) 452, 886, 1253, 1308, 1498, 1856, 2233, 2453, 2655
Sau96I GGNCC 4 cut(s) 55, 422, 1811, 1843
SchI GAGTC 4 cut(s) 1122, 1297, 1794, 2611
ScrFI CCNGG 3 cut(s) 298, 540, 595
SduI GDGCHC 2 cut(s) 2166, 2763
SfaNI GCATC 7 cut(s) 117, 142, 214, 670, 1294, 1576, 2550
SfcI CTRYAG 4 cut(s) 975, 1856, 1940, 2655
SfoI GGCGCC 1 cut(s) 626
SfuI TTCGAA 2 cut(s) 241, 489
SinI GGWCC 3 cut(s) 55, 1811, 1843
SmiMI CAYNNNNRTG 3 cut(s) 242, 468, 633
SmlI CTYRAG 3 cut(s) 50, 1556, 2461
SmoI CTYRAG 3 cut(s) 50, 1556, 2461
SpeI ACTAGT 1 cut(s) 2092
SsiI CCGC 8 cut(s) 21, 130, 155, 451, 1498, 2080, 2475, 2697
SspDI GGCGCC 1 cut(s) 624
SspI AATATT 4 cut(s) 458, 739, 919, 1688
SspMI CTAG 4 cut(s) 372, 1403, 1790, 2093
StyD4I CCNGG 3 cut(s) 296, 538, 593
StyI CCWWGG 1 cut(s) 1717
TaaI ACNGT 5 cut(s) 326, 608, 938, 1045, 1975
TaiI ACGT 4 cut(s) 105, 856, 1496, 1656
TaqI TCGA 6 cut(s) 241, 319, 489, 616, 1083, 2611
TatI WGTACW 3 cut(s) 1117, 1975, 2122
TauI GCSGC 2 cut(s) 454, 1500
TfiI GAWTC 9 cut(s) 71, 304, 406, 1145, 1330, 1485, 1592, 1967, 2773
TscAI CASTG 1 cut(s) 943
TseFI GTSAC 4 cut(s) 709, 1701, 2221, 2258
TseI GCWGC 7 cut(s) 885, 1252, 1307, 1855, 2232, 2452, 2654
Tsp45I GTSAC 4 cut(s) 709, 1701, 2221, 2258
TspDTI ATGAA 6 cut(s) 480, 972, 1410, 2018, 2324, 2479
TspGWI ACGGA 3 cut(s) 70, 999, 2755
TspRI CASTG 1 cut(s) 943
VpaK11BI GGWCC 3 cut(s) 55, 1811, 1843
XagI CCTNNNNNAGG 1 cut(s) 50
XapI RAATTY 7 cut(s) 965, 1063, 1466, 1955, 1980, 2199, 2466
XceI RCATGY 1 cut(s) 2759
XcmI CCANNNNNNNNNTGG 2 cut(s) 681, 1992
XmiI GTMKAC 2 cut(s) 1475, 2542
XmnI GAANNNNTTC 2 cut(s) 247, 1551
XspI CTAG 4 cut(s) 372, 1403, 1790, 2093
Zsp2I ATGCAT 1 cut(s) 2565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.