MD01G1086900.v1.1
BHLH Family

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000212
Physical Location & Seq
Forward (+)
19597538 .. 19599016
1479 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1086900.v1.1.491

Sequence Viewer

Length: 1479 bp
ATGGAAGAATTAATAATCTCCCCCTCTTCATCCTCATCCCTAGTCTCCTTGCCTCAAGAAAACCATCCATCCCTCCAACAAAGGCTCCAATTCATTCTTCAATCCCAGCCAGACTGGTGGTCATACGCCATCTTCTGGCAAACCTCGAACGACCACCTGGTGGACAATGGTTGTCTCTTCCTGACCTGGGGAGACGGCCATTTCCAACGCCCCAAAGACGCATCACCCAACAACCACCACCACAACCCCTACGAAGTTCTATCCGAAAGAAGGAAGATCCTCAAGGGCATCCAATCCCTCATCAACGACACCAACAATCCAGATAACCATTATCTCTACAACATGGGCTTAAACGCCAACACCACCAACATCGAGGGGTTCTTCGTCAGGTCGTTCGCTCGATCCTTCTCCGTCGGTGAAAGCATTAATACTAATGTTCCCGGCAAGGCTTTTAGCTCTGGATCTTTGGTGTGGCTGACTGGAAGCCATGAGCTCCAATCCTACGTCTGCGAAAGAGCAAAAGAAGCACAAATGCATGGGATTCAAACCCTAGTCTGCATCCCAACCCCCACCGGAGTTCTCGAGCTGGGTTCTTCAGATTTGATCAGAGAAAACTGGAACCTGGTTCAGCAAACGAAATCCTTATTCTGGTCGGACCTTGTTTCGGACCAACAAGAACCGGGAACCAGATCCCCCATCAATCTAATCAACCGAAACTTCTCCTTCTCCGACTTTGACATCATCGCCGGCGTTCAGGAAGAAAAAAACAAAAAGGAAGTCCCCTTGGATATAACGGTGATGAAGAAGACGTGCGGAAGAGCATGTCCGGGTTCCATACCGTTTTCGAATCTAAATAACCTTAACCCGGAACCTTCGGATTCGGAGTTTCCCAAACGGACGCCGAAAAAGCGAGGGCGAAAACCGGGCCTTGGGCTAGACACAACGTTGGACCACGTGGAAGCGGAGAGGCAGCGGCGGGAGAAGCTCAACCACCGGTTCTGCGCCCTACGAGCAGTGGTGCCGAACGTATCGAGAATGGACAAGGCATCGTTGCTGTCCGACGCCGTTTCGTACATCATCGAGCTGAAGAGCAAGGTGGACGAGATGGAGTCGCAGGTGCAGAGAGAGTCCAAGAAAGTGAAAGCCGAGACCGTCGACAACTTGGACAATCAGAGCAGCACGACGTCGGTGGATCAAACGCGGCCGCCCAACTCGTTGGTGAGTGGGTCTACGGGGCTCGAGGTGGAGGTGAAGATAGTGTGTTCGGACGCCATGATTAGGGTTCGGTCCGAGAATGTGAACTACCCGTCAGCTAGGTTAATGGCGGCGCTGCGTGACTTGGACTTTGAGATGTACCATGCGAGCTTGTCATGCATGAACGAGCTCATGCTGCAAGATGTTGTGGTGAAGGTGCCTGATAACATGAGAAGTGAAGAGAGTATCAAAGCTGCTCTTCTTAGAATATTAGATCAGAACTGA

Protein Analysis

493

Amino Acids

55.39

Weight (kDa)

5.9

Isoelectric Point (pI)

50.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH-MYC_N PF14215 25 - 216 9.5e-49 bHLH-MYC and R2R3-MYB transcription factors N-terminal
HLH PF00010 318 - 362 3.9e-11 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000212)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G00870
fragaria_vesca FvH4_5g10850 FvH4_5g10851 FvH4_5g23290 FvH4_7g17380
malus_domestica MD00G1137900.v1.1 MD01G1086800.v1.1 MD01G1086900.v1.1 MD03G1096500.v1.1 MD06G1119900.v1.1 MD06G1120000.v1.1 MD06G1120100.v1.1 MD06G1120200.v1.1 MD11G1120400.v1.1 MD11G1145000.v1.1 MD11G1254000.v1.1 MD11G1254100.v1.1 MD14G1126900.v1.1 MD14G1127000.v1.1 MD14G1137200.v1.1
prunus_persica Prupe.2G195300_v2.0.a1 Prupe.2G195300_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087900_v2.0.a1 Prupe.5G130300_v2.0.a1 Prupe.5G130400_v2.0.a1 Prupe.5G130500_v2.0.a1 Prupe.5G130500_v2.0.a1 Prupe.5G130600_v2.0.a1 Prupe.5G130700_v2.0.a1
pyrus_communis pycom01g09980 pycom06g11100 pycom06g11120 pycom06g11150 pycom11g10170 pycom11g22490 pycom14g11190 pycom14g11210
rosa_chinensis RchiOBHm_Chr1g0360811 RchiOBHm_Chr5g0055891 RchiOBHm_Chr6g0281801 RchiOBHm_Chr7g0187141 RchiOBHm_Chr7g0187261 RchiOBHm_Chr7g0212241 RchiOBHm_Chr7g0228701
rosa_laevigata RLG00000002910 RLG00000002911 RLG00000004796 RLG00000004799 RLG00000027784
rosa_multiflora Rmu_sc0000340.1_g000036 Rmu_sc0000378.1_g000013 Rmu_sc0000441.1_g000071 Rmu_sc0000559.1_g000036 Rmu_sc0000637.1_g000017 Rmu_sc0000765.1_g000001 Rmu_sc0000785.1_g000012 Rmu_sc0000854.1_g000018 Rmu_sc0000959.1_g000018 Rmu_sc0001018.1_g000022 Rmu_sc0001136.1_g000078 Rmu_sc0001433.1_g000037 Rmu_sc0001687.1_g000010 Rmu_sc0001716.1_g000014 Rmu_sc0001758.1_g000023 Rmu_sc0001758.1_g000031 Rmu_sc0001826.1_g000009 Rmu_sc0001850.1_g000027 Rmu_sc0001911.1_g000019 Rmu_sc0001937.1_g000001 Rmu_sc0002283.1_g000088 Rmu_sc0002532.1_g000058 Rmu_sc0002571.1_g000032 Rmu_sc0002888.1_g000014 Rmu_sc0002939.1_g000019 Rmu_sc0003197.1_g000007 Rmu_sc0003546.1_g000034 Rmu_sc0004002.1_g000003 Rmu_sc0004145.1_g000003 Rmu_sc0004301.1_g000013 Rmu_sc0004603.1_g000002 Rmu_sc0004748.1_g000007 Rmu_sc0004842.1_g000002 Rmu_sc0004990.1_g000012 Rmu_sc0005017.1_g000006 Rmu_sc0006343.1_g000001 Rmu_sc0006836.1_g000017 Rmu_sc0007491.1_g000003 Rmu_sc0008192.1_g000011 Rmu_sc0008877.1_g000007 Rmu_sc0010621.1_g000006 Rmu_sc0013354.1_g000017 Rmu_sc0013486.1_g000001 Rmu_sc0016511.1_g000001 Rmu_sc0021179.1_g000001 Rmu_sc0026563.1_g000001 Rmu_sc0027421.1_g000001 Rmu_sc0037114.1_g000001 Rmu_ssc0000022.1_g000039 Rmu_ssc0000050.1_g000048 Rmu_ssc0000141.1_g000004 Rmu_ssc0000280.1_g000002 Rmu_ssc0000387.1_g000024 Rmu_ssc0000459.1_g000030
rosa_roxburghii Rroxscaffold_3G00247010 Rroxscaffold_3G00267630 Rroxscaffold_3G00267670 Rroxscaffold_4G00295790 Rroxscaffold_4G00305930 Rroxscaffold_5G00346440 Rroxscaffold_7G00211970
rosa_rugosa Rorug01G0281800 Rorug02G0258400 Rorug04G0057400 Rorug04G0362000 Rorug04G0437300 Rorug06G0161400 Rorug06G0213300 Rorug06G0478800 Rorug06G0479300 Rorug06G0479300 Rorug07G0132200 Rorug07G0321200
rosa_samantha Rh1AG293700 Rh1BG258400 Rh1CG276200 Rh1DG288700 Rh7AG084400 Rh7AG084900 Rh7AG265100 Rh7BG078100 Rh7BG078600 Rh7CG085500 Rh7CG085800 Rh7CG281900 Rh7DG086500 Rh7DG086900 Rh7DG272300
rosa_wichuraiana Rw1G025990 Rw7G007440 Rw7G007460 Rw7G022700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1105
AatII GACGTC 1 cut(s) 1187
Acc36I ACCTGC 1 cut(s) 1105
AccB1I GGYRCC 2 cut(s) 1018, 1411
AccB7I CCANNNNNTGG 2 cut(s) 135, 160
AccI GTMKAC 2 cut(s) 1155, 1229
AccII CGCG 1 cut(s) 1201
AciI CCGC 7 cut(s) 813, 962, 973, 976, 1201, 1205, 1325
AclI AACGTT 1 cut(s) 944
AclWI GGATC 5 cut(s) 271, 396, 469, 684, 1200
AcoI YGGCCR 2 cut(s) 196, 1202
AcuI CTGAAG 2 cut(s) 579, 1106
AcvI CACGTG 1 cut(s) 955
AcyI GRCGYC 4 cut(s) 899, 1062, 1184, 1269
AdeI CACNNNGTG 1 cut(s) 160
AfaI GTAC 2 cut(s) 1073, 1355
AfiI CCNNNNNNNGG 9 cut(s) 135, 160, 187, 270, 648, 664, 865, 929, 1278
AgeI ACCGGT 1 cut(s) 993
AgsI TTSAA 2 cut(s) 101, 545
AhdI GACNNNNNGTC 1 cut(s) 118
AjiI CACGTC 1 cut(s) 810
AjnI CCWGG 3 cut(s) 156, 185, 621
AjuI GAANNNNNNNTTGG 2 cut(s) 81, 113
AluBI AGCT 9 cut(s) 456, 493, 586, 985, 1084, 1313, 1365, 1384, 1448
AluI AGCT 9 cut(s) 456, 493, 586, 985, 1084, 1313, 1365, 1384, 1448
Alw21I GWGCWC 2 cut(s) 495, 1386
Alw26I GTCTC 4 cut(s) 49, 179, 186, 1142
AlwI GGATC 5 cut(s) 271, 396, 469, 684, 1200
Ama87I CYCGRG 2 cut(s) 581, 1238
AoxI GGCC 3 cut(s) 196, 925, 1202
ApeKI GCWGC 5 cut(s) 970, 1176, 1330, 1390, 1448
ArsI GACNNNNNNTTYG 4 cut(s) 762, 794, 1328, 1360
AseI ATTAAT 2 cut(s) 11, 426
AsiGI ACCGGT 1 cut(s) 993
AspLEI GCGC 2 cut(s) 1004, 1330
AspS9I GGNCC 5 cut(s) 655, 667, 925, 949, 1287
AsuC2I CCSGG 5 cut(s) 441, 681, 828, 866, 924
AsuHPI GGTGA 6 cut(s) 216, 428, 808, 1231, 1261, 1417
AsuII TTCGAA 1 cut(s) 845
AvaI CYCGRG 2 cut(s) 581, 1238
AvaII GGWCC 4 cut(s) 655, 667, 949, 1287
BanI GGYRCC 2 cut(s) 1018, 1411
BanII GRGCYC 3 cut(s) 495, 1239, 1386
BbrPI CACGTG 1 cut(s) 955
BbsI GAAGAC 1 cut(s) 812
Bbv12I GWGCWC 2 cut(s) 495, 1386
BbvI GCAGC 5 cut(s) 982, 1188, 1317, 1377, 1435
BccI CCATC 5 cut(s) 72, 76, 137, 704, 1099
BceAI ACGGC 2 cut(s) 211, 1049
BciT130I CCWGG 3 cut(s) 158, 187, 623
BclI TGATCA 1 cut(s) 603
BcnI CCSGG 5 cut(s) 441, 681, 828, 866, 924
BcoDI GTCTC 4 cut(s) 49, 179, 186, 1142
BfaI CTAG 4 cut(s) 41, 551, 935, 1314
BfoI RGCGCY 1 cut(s) 1331
BfuAI ACCTGC 1 cut(s) 1105
BisI GCNGC 9 cut(s) 971, 974, 1177, 1202, 1205, 1326, 1331, 1391, 1449
BlsI GCNGC 9 cut(s) 972, 975, 1178, 1203, 1206, 1327, 1332, 1392, 1450
Bme1390I CCNGG 8 cut(s) 158, 187, 441, 623, 681, 828, 866, 924
Bme18I GGWCC 4 cut(s) 655, 667, 949, 1287
BmeRI GACNNNNNGTC 1 cut(s) 118
BmeT110I CYCGRG 2 cut(s) 581, 1238
BmgBI CACGTC 1 cut(s) 810
BmgT120I GGNCC 5 cut(s) 655, 667, 925, 949, 1287
BmiI GGNNCC 7 cut(s) 86, 620, 685, 832, 870, 1020, 1413
BmrFI CCNGG 8 cut(s) 158, 187, 441, 623, 681, 828, 866, 924
BmsI GCATC 4 cut(s) 230, 297, 567, 1055
BpiI GAAGAC 1 cut(s) 812
Bpu14I TTCGAA 1 cut(s) 845
BpuEI CTTGAG 2 cut(s) 39, 266
BpuMI CCSGG 5 cut(s) 441, 681, 828, 866, 924
BsaAI YACGTR 1 cut(s) 955
BsaHI GRCGYC 4 cut(s) 899, 1062, 1184, 1269
BsaI GGTCTC 1 cut(s) 1142
BsaJI CCNNGG 3 cut(s) 186, 783, 928
BsaWI WCCGGW 2 cut(s) 572, 993
BsaXI ACNNNNNCTCC 2 cut(s) 69, 99
Bsc4I CCNNNNNNNGG 9 cut(s) 135, 160, 187, 270, 648, 664, 865, 929, 1278
Bse118I RCCGGY 2 cut(s) 746, 993
Bse1I ACTGG 3 cut(s) 119, 484, 620
BseBI CCWGG 3 cut(s) 158, 187, 623
BseDI CCNNGG 3 cut(s) 186, 783, 928
BseGI GGATG 6 cut(s) 29, 35, 64, 68, 288, 558
BseLI CCNNNNNNNGG 9 cut(s) 135, 160, 187, 270, 648, 664, 865, 929, 1278
BseNI ACTGG 3 cut(s) 119, 484, 620
BseX3I CGGCCG 1 cut(s) 1202
BseXI GCAGC 5 cut(s) 982, 1188, 1317, 1377, 1435
BseYI CCCAGC 2 cut(s) 105, 586
BsgI GTGCAG 1 cut(s) 1139
Bsh1236I CGCG 1 cut(s) 1201
Bsh1285I CGRYCG 1 cut(s) 1205
BshFI GGCC 3 cut(s) 198, 927, 1204
BshNI GGYRCC 2 cut(s) 1018, 1411
BshTI ACCGGT 1 cut(s) 993
BsiEI CGRYCG 1 cut(s) 1205
BsiHKAI GWGCWC 2 cut(s) 495, 1386
BsiHKCI CYCGRG 2 cut(s) 581, 1238
BsiSI CCGG 8 cut(s) 441, 573, 680, 747, 827, 866, 923, 994
BslFI GGGAC 1 cut(s) 764
BslI CCNNNNNNNGG 9 cut(s) 135, 160, 187, 270, 648, 664, 865, 929, 1278
BsmAI GTCTC 4 cut(s) 49, 179, 186, 1142
BsmBI CGTCTC 1 cut(s) 186
BsmFI GGGAC 1 cut(s) 764
BsnI GGCC 3 cut(s) 198, 927, 1204
Bso31I GGTCTC 1 cut(s) 1142
BsoBI CYCGRG 2 cut(s) 581, 1238
Bsp119I TTCGAA 1 cut(s) 845
Bsp1286I GDGCHC 3 cut(s) 495, 1239, 1386
Bsp143I GATC 7 cut(s) 276, 401, 461, 603, 689, 1192, 1468
BspACI CCGC 7 cut(s) 813, 962, 973, 976, 1201, 1205, 1325
BspANI GGCC 3 cut(s) 198, 927, 1204
BspFNI CGCG 1 cut(s) 1201
BspLI GGNNCC 7 cut(s) 86, 620, 685, 832, 870, 1020, 1413
BspMI ACCTGC 1 cut(s) 1105
BspPI GGATC 5 cut(s) 271, 396, 469, 684, 1200
BspQI GCTCTTC 3 cut(s) 811, 1082, 1458
BspT104I TTCGAA 1 cut(s) 845
BspT107I GGYRCC 2 cut(s) 1018, 1411
BspTNI GGTCTC 1 cut(s) 1142
BsrFI RCCGGY 2 cut(s) 746, 993
BsrI ACTGG 3 cut(s) 119, 484, 620
BssAI RCCGGY 2 cut(s) 746, 993
BssECI CCNNGG 3 cut(s) 186, 783, 928
BssMI GATC 7 cut(s) 276, 401, 461, 603, 689, 1192, 1468
BssNI GRCGYC 4 cut(s) 899, 1062, 1184, 1269
BssT1I CCWWGG 2 cut(s) 783, 928
Bst2UI CCWGG 3 cut(s) 158, 187, 623
Bst4CI ACNGT 3 cut(s) 796, 840, 1153
Bst6I CTCTTC 6 cut(s) 31, 182, 811, 1082, 1428, 1458
BstACI GRCGYC 4 cut(s) 899, 1062, 1184, 1269
BstBAI YACGTR 1 cut(s) 955
BstBI TTCGAA 1 cut(s) 845
BstC8I GCNNGC 2 cut(s) 748, 1363
BstDEI CTNAG 1 cut(s) 1457
BstF5I GGATG 6 cut(s) 29, 35, 64, 68, 288, 558
BstFNI CGCG 1 cut(s) 1201
BstH2I RGCGCY 1 cut(s) 1331
BstHHI GCGC 2 cut(s) 1004, 1330
BstKTI GATC 7 cut(s) 279, 404, 464, 606, 692, 1195, 1471
BstMAI GTCTC 4 cut(s) 49, 179, 186, 1142
BstMBI GATC 7 cut(s) 276, 401, 461, 603, 689, 1192, 1468
BstMCI CGRYCG 1 cut(s) 1205
BstMWI GCNNNNNNNGC 6 cut(s) 524, 907, 982, 1010, 1371, 1390
BstNI CCWGG 3 cut(s) 158, 187, 623
BstNSI RCATGY 1 cut(s) 825
BstSCI CCNGG 8 cut(s) 156, 185, 439, 621, 679, 826, 864, 922
BstUI CGCG 1 cut(s) 1201
BstV1I GCAGC 5 cut(s) 982, 1188, 1317, 1377, 1435
BstV2I GAAGAC 1 cut(s) 812
BstX2I RGATCY 3 cut(s) 276, 461, 689
BstXI CCANNNNNNTGG 2 cut(s) 117, 1216
BstYI RGATCY 3 cut(s) 276, 461, 689
BstZI CGGCCG 1 cut(s) 1202
BsuRI GGCC 3 cut(s) 198, 927, 1204
BtgZI GCGATG 1 cut(s) 727
BtrI CACGTC 1 cut(s) 810
BtsCI GGATG 6 cut(s) 29, 35, 64, 68, 288, 558
BtsI GCAGTG 1 cut(s) 1020
BtsIMutI CAGTG 1 cut(s) 1020
BveI ACCTGC 1 cut(s) 1105
Cac8I GCNNGC 2 cut(s) 748, 1363
CciNI GCGGCCGC 1 cut(s) 1202
CfoI GCGC 2 cut(s) 1004, 1330
Cfr10I RCCGGY 2 cut(s) 746, 993
Cfr13I GGNCC 5 cut(s) 655, 667, 925, 949, 1287
CpoI CGGWCCG 1 cut(s) 1287
CseI GACGC 4 cut(s) 227, 907, 1070, 1277
CsiI ACCWGGT 2 cut(s) 156, 621
Csp6I GTAC 2 cut(s) 1072, 1354
CspAI ACCGGT 1 cut(s) 993
CspI CGGWCCG 1 cut(s) 1287
CviQI GTAC 2 cut(s) 1072, 1354
DdeI CTNAG 1 cut(s) 1457
DpnI GATC 7 cut(s) 278, 403, 463, 605, 691, 1194, 1470
DpnII GATC 7 cut(s) 276, 401, 461, 603, 689, 1192, 1468
DraIII CACNNNGTG 1 cut(s) 160
DriI GACNNNNNGTC 1 cut(s) 118
EaeI YGGCCR 2 cut(s) 196, 1202
EagI CGGCCG 1 cut(s) 1202
Eam1104I CTCTTC 6 cut(s) 31, 182, 811, 1082, 1428, 1458
Eam1105I GACNNNNNGTC 1 cut(s) 118
EarI CTCTTC 6 cut(s) 31, 182, 811, 1082, 1428, 1458
Ecl136II GAGCTC 2 cut(s) 493, 1384
EclXI CGGCCG 1 cut(s) 1202
Eco130I CCWWGG 2 cut(s) 783, 928
Eco24I GRGCYC 3 cut(s) 495, 1239, 1386
Eco31I GGTCTC 1 cut(s) 1142
Eco47I GGWCC 4 cut(s) 655, 667, 949, 1287
Eco52I CGGCCG 1 cut(s) 1202
Eco53kI GAGCTC 2 cut(s) 493, 1384
Eco57I CTGAAG 2 cut(s) 579, 1106
Eco72I CACGTG 1 cut(s) 955
Eco88I CYCGRG 2 cut(s) 581, 1238
EcoICRI GAGCTC 2 cut(s) 493, 1384
EcoRII CCWGG 3 cut(s) 156, 185, 621
EcoT14I CCWWGG 2 cut(s) 783, 928
EcoT22I ATGCAT 2 cut(s) 537, 1376
EcoT38I GRGCYC 3 cut(s) 495, 1239, 1386
ErhI CCWWGG 2 cut(s) 783, 928
Esp3I CGTCTC 1 cut(s) 186
FalI AAGNNNNNCTT 2 cut(s) 1437, 1469
FaqI GGGAC 1 cut(s) 764
FauI CCCGC 1 cut(s) 969
FbaI TGATCA 1 cut(s) 603
FblI GTMKAC 2 cut(s) 1155, 1229
Fnu4HI GCNGC 9 cut(s) 971, 974, 1177, 1202, 1205, 1326, 1331, 1391, 1449
FokI GGATG 6 cut(s) 16, 22, 51, 55, 275, 545
FriOI GRGCYC 3 cut(s) 495, 1239, 1386
Fsp4HI GCNGC 9 cut(s) 971, 974, 1177, 1202, 1205, 1326, 1331, 1391, 1449
FspBI CTAG 4 cut(s) 41, 551, 935, 1314
GlaI GCGC 2 cut(s) 1003, 1329
GluI GCNGC 9 cut(s) 971, 974, 1177, 1202, 1205, 1326, 1331, 1391, 1449
GsaI CCCAGC 2 cut(s) 109, 590
HaeII RGCGCY 1 cut(s) 1331
HaeIII GGCC 3 cut(s) 198, 927, 1204
HapII CCGG 8 cut(s) 441, 573, 680, 747, 827, 866, 923, 994
HgaI GACGC 4 cut(s) 227, 907, 1070, 1277
HhaI GCGC 2 cut(s) 1004, 1330
Hin1I GRCGYC 4 cut(s) 899, 1062, 1184, 1269
Hin6I GCGC 2 cut(s) 1002, 1328
HinP1I GCGC 2 cut(s) 1002, 1328
HincII GTYRAC 1 cut(s) 1156
HindII GTYRAC 1 cut(s) 1156
HinfI GANTC 5 cut(s) 541, 847, 878, 1109, 1127
HpaII CCGG 8 cut(s) 441, 573, 680, 747, 827, 866, 923, 994
HphI GGTGA 6 cut(s) 216, 428, 808, 1231, 1261, 1417
Hpy166II GTNNAC 5 cut(s) 163, 1099, 1156, 1230, 1300
Hpy188III TCNNGA 7 cut(s) 56, 181, 320, 459, 581, 755, 1032
Hpy8I GTNNAC 5 cut(s) 163, 1099, 1156, 1230, 1300
Hpy99I CGWCG 5 cut(s) 416, 1064, 1157, 1186, 1189
HpyAV CCTTC 5 cut(s) 264, 415, 733, 882, 1402
HpyCH4III ACNGT 3 cut(s) 796, 840, 1153
HpyCH4IV ACGT 6 cut(s) 504, 809, 944, 954, 1026, 1184
HpyCH4V TGCA 5 cut(s) 535, 558, 1120, 1374, 1393
HpyF10VI GCNNNNNNNGC 6 cut(s) 524, 907, 982, 1010, 1371, 1390
HpyF3I CTNAG 1 cut(s) 1457
HpySE526I ACGT 6 cut(s) 504, 809, 944, 954, 1026, 1184
Hsp92I GRCGYC 4 cut(s) 899, 1062, 1184, 1269
HspAI GCGC 2 cut(s) 1002, 1328
KroI GCCGGC 1 cut(s) 746
KroNI GCCGGC 1 cut(s) 748
Ksp22I TGATCA 1 cut(s) 603
Kzo9I GATC 7 cut(s) 276, 401, 461, 603, 689, 1192, 1468
LguI GCTCTTC 3 cut(s) 811, 1082, 1458
LmnI GCTCC 2 cut(s) 90, 498
Lsp1109I GCAGC 5 cut(s) 982, 1188, 1317, 1377, 1435
LweI GCATC 4 cut(s) 230, 297, 567, 1055
MabI ACCWGGT 2 cut(s) 156, 621
MaeI CTAG 4 cut(s) 41, 551, 935, 1314
MaeII ACGT 6 cut(s) 504, 809, 944, 954, 1026, 1184
MaeIII GTNAC 1 cut(s) 1334
MalI GATC 7 cut(s) 278, 403, 463, 605, 691, 1194, 1470
MboI GATC 7 cut(s) 276, 401, 461, 603, 689, 1192, 1468
MflI RGATCY 3 cut(s) 276, 461, 689
MhlI GDGCHC 3 cut(s) 495, 1239, 1386
MluCI AATT 2 cut(s) 8, 89
MlyI GAGTC 2 cut(s) 1118, 1136
MmeI TCCRAC 6 cut(s) 100, 229, 633, 753, 927, 1083
Mph1103I ATGCAT 2 cut(s) 537, 1376
MreI CGCCGGCG 1 cut(s) 746
MroNI GCCGGC 1 cut(s) 746
MseI TTAA 5 cut(s) 11, 350, 426, 861, 1319
MspA1I CMGCKG 1 cut(s) 973
MspI CCGG 8 cut(s) 441, 573, 680, 747, 827, 866, 923, 994
MspR9I CCNGG 8 cut(s) 158, 187, 441, 623, 681, 828, 866, 924
MvaI CCWGG 3 cut(s) 158, 187, 623
MvnI CGCG 1 cut(s) 1201
MwoI GCNNNNNNNGC 6 cut(s) 524, 907, 982, 1010, 1371, 1390
NaeI GCCGGC 1 cut(s) 748
NciI CCSGG 5 cut(s) 441, 681, 828, 866, 924
NdeII GATC 7 cut(s) 276, 401, 461, 603, 689, 1192, 1468
NgoMIV GCCGGC 1 cut(s) 746
NlaIV GGNNCC 7 cut(s) 86, 620, 685, 832, 870, 1020, 1413
NmeAIII GCCGAG 1 cut(s) 1171
NmuCI GTSAC 1 cut(s) 1334
NotI GCGGCCGC 1 cut(s) 1202
NsiI ATGCAT 2 cut(s) 537, 1376
NspI RCATGY 1 cut(s) 825
NspV TTCGAA 1 cut(s) 845
PaeR7I CTCGAG 2 cut(s) 581, 1238
PaqCI CACCTGC 1 cut(s) 1105
PciSI GCTCTTC 3 cut(s) 811, 1082, 1458
PdiI GCCGGC 1 cut(s) 748
PfeI GAWTC 3 cut(s) 541, 847, 878
PflMI CCANNNNNTGG 2 cut(s) 135, 160
PinAI ACCGGT 1 cut(s) 993
PkrI GCNGC 9 cut(s) 972, 975, 1178, 1203, 1206, 1327, 1332, 1392, 1450
PleI GAGTC 2 cut(s) 1117, 1135
PmaCI CACGTG 1 cut(s) 955
PmlI CACGTG 1 cut(s) 955
PpsI GAGTC 2 cut(s) 1117, 1135
Ppu21I YACGTR 1 cut(s) 955
PshBI ATTAAT 2 cut(s) 11, 426
Psp124BI GAGCTC 2 cut(s) 495, 1386
Psp1406I AACGTT 1 cut(s) 944
Psp6I CCWGG 3 cut(s) 156, 185, 621
PspCI CACGTG 1 cut(s) 955
PspFI CCCAGC 2 cut(s) 105, 586
PspGI CCWGG 3 cut(s) 156, 185, 621
PspN4I GGNNCC 7 cut(s) 86, 620, 685, 832, 870, 1020, 1413
PspPI GGNCC 5 cut(s) 655, 667, 925, 949, 1287
PspXI VCTCGAGB 1 cut(s) 1238
PsuI RGATCY 3 cut(s) 276, 461, 689
RsaI GTAC 2 cut(s) 1073, 1355
RsaNI GTAC 2 cut(s) 1072, 1354
Rsr2I CGGWCCG 1 cut(s) 1287
RsrII CGGWCCG 1 cut(s) 1287
SacI GAGCTC 2 cut(s) 495, 1386
SalI GTCGAC 1 cut(s) 1154
SapI GCTCTTC 3 cut(s) 811, 1082, 1458
SaqAI TTAA 5 cut(s) 11, 350, 426, 861, 1319
SatI GCNGC 9 cut(s) 971, 974, 1177, 1202, 1205, 1326, 1331, 1391, 1449
Sau3AI GATC 7 cut(s) 276, 401, 461, 603, 689, 1192, 1468
Sau96I GGNCC 5 cut(s) 655, 667, 925, 949, 1287
SchI GAGTC 2 cut(s) 1118, 1136
ScrFI CCNGG 8 cut(s) 158, 187, 441, 623, 681, 828, 866, 924
SduI GDGCHC 3 cut(s) 495, 1239, 1386
SexAI ACCWGGT 2 cut(s) 156, 621
SfaNI GCATC 4 cut(s) 230, 297, 567, 1055
Sfr274I CTCGAG 2 cut(s) 581, 1238
SfuI TTCGAA 1 cut(s) 845
SgrAI CRCCGGYG 1 cut(s) 746
SinI GGWCC 4 cut(s) 655, 667, 949, 1287
SlaI CTCGAG 2 cut(s) 581, 1238
SmlI CTYRAG 4 cut(s) 54, 281, 581, 1238
SmoI CTYRAG 4 cut(s) 54, 281, 581, 1238
Sse9I AATT 2 cut(s) 8, 89
SsiI CCGC 7 cut(s) 813, 962, 973, 976, 1201, 1205, 1325
SspI AATATT 1 cut(s) 1464
SspMI CTAG 4 cut(s) 41, 551, 935, 1314
SstI GAGCTC 2 cut(s) 495, 1386
StyD4I CCNGG 8 cut(s) 156, 185, 439, 621, 679, 826, 864, 922
StyI CCWWGG 2 cut(s) 783, 928
TaaI ACNGT 3 cut(s) 796, 840, 1153
TaiI ACGT 6 cut(s) 507, 812, 947, 957, 1029, 1187
TaqI TCGA 9 cut(s) 146, 372, 400, 582, 845, 1031, 1080, 1155, 1239
TaqII GACCGA 1 cut(s) 1275
TasI AATT 2 cut(s) 8, 89
TauI GCSGC 4 cut(s) 976, 1204, 1207, 1328
TfiI GAWTC 3 cut(s) 541, 847, 878
Tru1I TTAA 5 cut(s) 11, 350, 426, 861, 1319
Tru9I TTAA 5 cut(s) 11, 350, 426, 861, 1319
TscAI CASTG 1 cut(s) 1020
TseFI GTSAC 1 cut(s) 1334
TseI GCWGC 5 cut(s) 970, 1176, 1330, 1390, 1448
Tsp45I GTSAC 1 cut(s) 1334
TspDTI ATGAA 4 cut(s) 18, 82, 815, 1391
TspGWI ACGGA 2 cut(s) 400, 910
TspRI CASTG 1 cut(s) 1020
Van91I CCANNNNNTGG 2 cut(s) 135, 160
VpaK11BI GGWCC 4 cut(s) 655, 667, 949, 1287
VspI ATTAAT 2 cut(s) 11, 426
XceI RCATGY 1 cut(s) 825
XhoI CTCGAG 2 cut(s) 581, 1238
XmiI GTMKAC 2 cut(s) 1155, 1229
XspI CTAG 4 cut(s) 41, 551, 935, 1314
ZraI GACGTC 1 cut(s) 1185
Zsp2I ATGCAT 2 cut(s) 537, 1376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.