transcription factor
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 241 |
| AccII | CGCG | 1 cut(s) | 47 |
| AciI | CCGC | 4 cut(s) | 23, 35, 88, 172 |
| AclWI | GGATC | 1 cut(s) | 43 |
| AcoI | YGGCCR | 1 cut(s) | 173 |
| AcyI | GRCGYC | 1 cut(s) | 242 |
| AfaI | GTAC | 2 cut(s) | 96, 126 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 58, 147, 269 |
| AgsI | TTSAA | 2 cut(s) | 145, 154 |
| AjnI | CCWGG | 1 cut(s) | 339 |
| AluBI | AGCT | 3 cut(s) | 71, 250, 324 |
| AluI | AGCT | 3 cut(s) | 71, 250, 324 |
| AlwI | GGATC | 1 cut(s) | 43 |
| AoxI | GGCC | 6 cut(s) | 82, 89, 119, 173, 212, 294 |
| ApeKI | GCWGC | 2 cut(s) | 68, 321 |
| AspLEI | GCGC | 1 cut(s) | 244 |
| AspS9I | GGNCC | 2 cut(s) | 90, 295 |
| AsuC2I | CCSGG | 1 cut(s) | 270 |
| BanI | GGYRCC | 1 cut(s) | 241 |
| BbsI | GAAGAC | 1 cut(s) | 364 |
| BbvI | GCAGC | 2 cut(s) | 80, 333 |
| BccI | CCATC | 1 cut(s) | 232 |
| BciT130I | CCWGG | 1 cut(s) | 341 |
| BclI | TGATCA | 1 cut(s) | 54 |
| BcnI | CCSGG | 1 cut(s) | 270 |
| BfaI | CTAG | 1 cut(s) | 382 |
| BfoI | RGCGCY | 1 cut(s) | 245 |
| BisI | GCNGC | 5 cut(s) | 36, 69, 89, 173, 322 |
| BlsI | GCNGC | 5 cut(s) | 37, 70, 90, 174, 323 |
| Bme1390I | CCNGG | 2 cut(s) | 270, 341 |
| BmgT120I | GGNCC | 2 cut(s) | 90, 295 |
| BmiI | GGNNCC | 2 cut(s) | 243, 297 |
| BmrFI | CCNGG | 2 cut(s) | 270, 341 |
| BpiI | GAAGAC | 1 cut(s) | 364 |
| BpuEI | CTTGAG | 1 cut(s) | 301 |
| BpuMI | CCSGG | 1 cut(s) | 270 |
| BsaBI | GATNNNNATC | 1 cut(s) | 53 |
| BsaHI | GRCGYC | 1 cut(s) | 242 |
| BsaJI | CCNNGG | 1 cut(s) | 339 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 58, 147, 269 |
| Bse1I | ACTGG | 1 cut(s) | 122 |
| Bse8I | GATNNNNATC | 1 cut(s) | 53 |
| BseBI | CCWGG | 1 cut(s) | 341 |
| BseDI | CCNNGG | 1 cut(s) | 339 |
| BseJI | GATNNNNATC | 1 cut(s) | 53 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 58, 147, 269 |
| BseMII | CTCAG | 1 cut(s) | 23 |
| BseNI | ACTGG | 1 cut(s) | 122 |
| BseXI | GCAGC | 2 cut(s) | 80, 333 |
| BsgI | GTGCAG | 1 cut(s) | 87 |
| Bsh1236I | CGCG | 1 cut(s) | 47 |
| BshFI | GGCC | 6 cut(s) | 84, 91, 121, 175, 214, 296 |
| BshNI | GGYRCC | 1 cut(s) | 241 |
| BsiSI | CCGG | 1 cut(s) | 270 |
| BslFI | GGGAC | 2 cut(s) | 292, 322 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 58, 147, 269 |
| BsmFI | GGGAC | 2 cut(s) | 292, 322 |
| BsnI | GGCC | 6 cut(s) | 84, 91, 121, 175, 214, 296 |
| Bsp143I | GATC | 3 cut(s) | 12, 48, 54 |
| Bsp68I | TCGCGA | 1 cut(s) | 47 |
| BspACI | CCGC | 4 cut(s) | 23, 35, 88, 172 |
| BspANI | GGCC | 6 cut(s) | 84, 91, 121, 175, 214, 296 |
| BspCNI | CTCAG | 1 cut(s) | 22 |
| BspFNI | CGCG | 1 cut(s) | 47 |
| BspLI | GGNNCC | 2 cut(s) | 243, 297 |
| BspPI | GGATC | 1 cut(s) | 43 |
| BspT107I | GGYRCC | 1 cut(s) | 241 |
| BsrI | ACTGG | 1 cut(s) | 122 |
| BssECI | CCNNGG | 1 cut(s) | 339 |
| BssMI | GATC | 3 cut(s) | 12, 48, 54 |
| BssNI | GRCGYC | 1 cut(s) | 242 |
| Bst2UI | CCWGG | 1 cut(s) | 341 |
| Bst4CI | ACNGT | 1 cut(s) | 306 |
| BstACI | GRCGYC | 1 cut(s) | 242 |
| BstC8I | GCNNGC | 2 cut(s) | 86, 170 |
| BstDEI | CTNAG | 1 cut(s) | 9 |
| BstFNI | CGCG | 1 cut(s) | 47 |
| BstH2I | RGCGCY | 1 cut(s) | 245 |
| BstHHI | GCGC | 1 cut(s) | 244 |
| BstKTI | GATC | 3 cut(s) | 15, 51, 57 |
| BstMBI | GATC | 3 cut(s) | 12, 48, 54 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 44, 256, 265, 274, 283, 365 |
| BstNI | CCWGG | 1 cut(s) | 341 |
| BstNSI | RCATGY | 3 cut(s) | 109, 172, 259 |
| BstSCI | CCNGG | 2 cut(s) | 268, 339 |
| BstUI | CGCG | 1 cut(s) | 47 |
| BstV1I | GCAGC | 2 cut(s) | 80, 333 |
| BstV2I | GAAGAC | 1 cut(s) | 364 |
| BsuRI | GGCC | 6 cut(s) | 84, 91, 121, 175, 214, 296 |
| BtuMI | TCGCGA | 1 cut(s) | 47 |
| Cac8I | GCNNGC | 2 cut(s) | 86, 170 |
| CfoI | GCGC | 1 cut(s) | 244 |
| Cfr13I | GGNCC | 2 cut(s) | 90, 295 |
| Csp6I | GTAC | 2 cut(s) | 95, 125 |
| CviAII | CATG | 7 cut(s) | 58, 106, 128, 169, 190, 208, 256 |
| CviQI | GTAC | 2 cut(s) | 95, 125 |
| DdeI | CTNAG | 1 cut(s) | 9 |
| DinI | GGCGCC | 1 cut(s) | 243 |
| DpnI | GATC | 3 cut(s) | 14, 50, 56 |
| DpnII | GATC | 3 cut(s) | 12, 48, 54 |
| EaeI | YGGCCR | 1 cut(s) | 173 |
| EcoRII | CCWGG | 1 cut(s) | 339 |
| EgeI | GGCGCC | 1 cut(s) | 243 |
| EheI | GGCGCC | 1 cut(s) | 243 |
| FaeI | CATG | 7 cut(s) | 61, 109, 131, 172, 193, 211, 259 |
| FaqI | GGGAC | 2 cut(s) | 292, 322 |
| FatI | CATG | 7 cut(s) | 57, 105, 127, 168, 189, 207, 255 |
| FbaI | TGATCA | 1 cut(s) | 54 |
| Fnu4HI | GCNGC | 5 cut(s) | 36, 69, 89, 173, 322 |
| Fsp4HI | GCNGC | 5 cut(s) | 36, 69, 89, 173, 322 |
| FspBI | CTAG | 1 cut(s) | 382 |
| GlaI | GCGC | 1 cut(s) | 243 |
| GluI | GCNGC | 5 cut(s) | 36, 69, 89, 173, 322 |
| HaeII | RGCGCY | 1 cut(s) | 245 |
| HaeIII | GGCC | 6 cut(s) | 84, 91, 121, 175, 214, 296 |
| HapII | CCGG | 1 cut(s) | 270 |
| HhaI | GCGC | 1 cut(s) | 244 |
| Hin1I | GRCGYC | 1 cut(s) | 242 |
| Hin1II | CATG | 7 cut(s) | 61, 109, 131, 172, 193, 211, 259 |
| Hin6I | GCGC | 1 cut(s) | 242 |
| HinP1I | GCGC | 1 cut(s) | 242 |
| HinfI | GANTC | 1 cut(s) | 351 |
| HpaII | CCGG | 1 cut(s) | 270 |
| Hpy188I | TCNGA | 1 cut(s) | 12 |
| Hpy188III | TCNNGA | 3 cut(s) | 46, 52, 229 |
| Hpy99I | CGWCG | 1 cut(s) | 368 |
| HpyAV | CCTTC | 1 cut(s) | 74 |
| HpyCH4III | ACNGT | 1 cut(s) | 306 |
| HpyCH4V | TGCA | 2 cut(s) | 68, 109 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 44, 256, 265, 274, 283, 365 |
| HpyF3I | CTNAG | 1 cut(s) | 9 |
| Hsp92I | GRCGYC | 1 cut(s) | 242 |
| Hsp92II | CATG | 7 cut(s) | 61, 109, 131, 172, 193, 211, 259 |
| HspAI | GCGC | 1 cut(s) | 242 |
| KasI | GGCGCC | 1 cut(s) | 241 |
| Ksp22I | TGATCA | 1 cut(s) | 54 |
| Kzo9I | GATC | 3 cut(s) | 12, 48, 54 |
| LpnPI | CCDG | 7 cut(s) | 65, 98, 135, 283, 300, 326, 353 |
| Lsp1109I | GCAGC | 2 cut(s) | 80, 333 |
| MaeI | CTAG | 1 cut(s) | 382 |
| MaeIII | GTNAC | 4 cut(s) | 27, 157, 196, 229 |
| MalI | GATC | 3 cut(s) | 14, 50, 56 |
| MboI | GATC | 3 cut(s) | 12, 48, 54 |
| MboII | GAAGA | 1 cut(s) | 364 |
| Mly113I | GGCGCC | 1 cut(s) | 242 |
| MmeI | TCCRAC | 2 cut(s) | 55, 328 |
| MnlI | CCTC | 3 cut(s) | 108, 111, 204 |
| MspA1I | CMGCKG | 2 cut(s) | 71, 88 |
| MspI | CCGG | 1 cut(s) | 270 |
| MspR9I | CCNGG | 2 cut(s) | 270, 341 |
| MvaI | CCWGG | 1 cut(s) | 341 |
| MvnI | CGCG | 1 cut(s) | 47 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 44, 256, 265, 274, 283, 365 |
| NarI | GGCGCC | 1 cut(s) | 242 |
| NciI | CCSGG | 1 cut(s) | 270 |
| NdeII | GATC | 3 cut(s) | 12, 48, 54 |
| NlaIII | CATG | 7 cut(s) | 61, 109, 131, 172, 193, 211, 259 |
| NlaIV | GGNNCC | 2 cut(s) | 243, 297 |
| NmuCI | GTSAC | 3 cut(s) | 157, 196, 229 |
| NruI | TCGCGA | 1 cut(s) | 47 |
| NspI | RCATGY | 3 cut(s) | 109, 172, 259 |
| PaeI | GCATGC | 1 cut(s) | 172 |
| PfeI | GAWTC | 1 cut(s) | 351 |
| PflFI | GACNNNGTC | 1 cut(s) | 304 |
| PkrI | GCNGC | 5 cut(s) | 37, 70, 90, 174, 323 |
| PluTI | GGCGCC | 1 cut(s) | 245 |
| Psp6I | CCWGG | 1 cut(s) | 339 |
| PspGI | CCWGG | 1 cut(s) | 339 |
| PspN4I | GGNNCC | 2 cut(s) | 243, 297 |
| PspPI | GGNCC | 2 cut(s) | 90, 295 |
| PsyI | GACNNNGTC | 1 cut(s) | 304 |
| PvuII | CAGCTG | 1 cut(s) | 71 |
| RruI | TCGCGA | 1 cut(s) | 47 |
| RsaI | GTAC | 2 cut(s) | 96, 126 |
| RsaNI | GTAC | 2 cut(s) | 95, 125 |
| SatI | GCNGC | 5 cut(s) | 36, 69, 89, 173, 322 |
| Sau3AI | GATC | 3 cut(s) | 12, 48, 54 |
| Sau96I | GGNCC | 2 cut(s) | 90, 295 |
| ScrFI | CCNGG | 2 cut(s) | 270, 341 |
| SetI | ASST | 4 cut(s) | 73, 100, 252, 326 |
| SfoI | GGCGCC | 1 cut(s) | 243 |
| SmlI | CTYRAG | 1 cut(s) | 316 |
| SmoI | CTYRAG | 1 cut(s) | 316 |
| SphI | GCATGC | 1 cut(s) | 172 |
| SsiI | CCGC | 4 cut(s) | 23, 35, 88, 172 |
| SspDI | GGCGCC | 1 cut(s) | 241 |
| SspMI | CTAG | 1 cut(s) | 382 |
| StyD4I | CCNGG | 2 cut(s) | 268, 339 |
| TaaI | ACNGT | 1 cut(s) | 306 |
| TaqI | TCGA | 2 cut(s) | 100, 252 |
| TatI | WGTACW | 1 cut(s) | 124 |
| TauI | GCSGC | 3 cut(s) | 38, 91, 175 |
| TfiI | GAWTC | 1 cut(s) | 351 |
| TseFI | GTSAC | 3 cut(s) | 157, 196, 229 |
| TseI | GCWGC | 2 cut(s) | 68, 321 |
| Tsp45I | GTSAC | 3 cut(s) | 157, 196, 229 |
| TspDTI | ATGAA | 2 cut(s) | 144, 206 |
| TspGWI | ACGGA | 1 cut(s) | 324 |
| Tth111I | GACNNNGTC | 1 cut(s) | 304 |
| XceI | RCATGY | 3 cut(s) | 109, 172, 259 |
| XspI | CTAG | 1 cut(s) | 382 |