MD06G1120100.v1.1
BHLH Family

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0000212
Physical Location & Seq
Forward (+)
25971717 .. 25973613
1897 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1120100.v1.1.491

Sequence Viewer

Length: 1404 bp
ATGGAGGAAATCAATTCCTCCTGGTCTTCATACAATTTTGGTCAAGAAAACTCACTCACACTTCAACGTCTGCAGTTCATAATTCAGAACAGGCCCGAATGGTGGGTCTATTCAATTTTCTGGCAAGCCTCAAAAGACAGCAATGGCCTAGTATATTTGTCGTGGGCTGGTGGCCATTTTCGAAGCACTAGGGACTTGGCCTCCAAACGAAGTTACAACAAAGCCAATGATTTCGATGTTGTGGAGAGGAAGAAGGGGATTAGAAAAGAAGTTGAAGCTCTTTTCTATGAACACATGGATTTGGATGGTGGAGACGTTACCGACGCTGAGTGGTTTTACTTTTACACTGCCTCTTTAACCCAGTCATTTACTGCCAGCCACGGTAACATTCTGGGTGGTACGTTTTGTTCTGGTGATTTTGTTTGGCTGGCAGGTGATCATGAGCTTCAGTTCTATGAGTGTGAGAGAGTGAAGGAAGCAAGAATGCATGGAATTCACACTTTGGTTTGTATTGCAACTTCATGCGGAGTACTTGAACTCGCTTCTTTCGATGTGATCAAAGTAGATTGGGGTCTTGTGGAGATGTCAAAATCGCTTTTTGGATCAAAGAACAACAACAGTGTCTCAAAGCAGCTGTGCAGCAGCCGTGAGGACCATGTACTTGTTCCGCTACTAGAAAGGGGAATGTTTTCAGGAGCTGAACAAATAGAGTGGACAACACAAGGTGGTACAAAAGAAGTAGATCCTGTGACTATAGGTGGATCACCATCCGACTCTGTTGGAAATTTCACCTCTGAGAATATGGAGAACATACGACTGAAAAAGAGAGGACGATCATCAAACAGTGAAACTGGTAGATCAGAAACACCAATAAACCATGTTGAGGCAGAAAGACAAAGGCGAGAGAAGCTTAACCATCGATTCTATGCCCTCCGTTCCGTCGTTCCCAATGTGTCCAGAATGGACAAAGCTTCTTTGCTTTCTGATGCAGTTGCATACATCAATGAGCTGAAAGCAAGGGTTGTGCAACTGGAGGCCAAAATCCAAGCACAACCTGGAAAGCCCGTTGTTGACATTGGAGGCCAAACCACCTGCTCCATAGTTGATCATCATCAAACAAGGCCACGGCAATCATCAAGTTATAATAATAGAGCAGCTGGGGCTATGAAAATAGATGTGAAGATCGTAGGCTCAGAAGCCATGATACGAGTTCAGTGTCCGGACTGCGACTATCCTAATGCTAGATTGATGAATGCACTCAAAAGCCTGGAGTTACAAGTTTATCATGCAAGCATATCAAGTGTAAAAGAGTTGATGCTTCAAGATGTTGTGGCAAGAGTGCCTCAGGGTTTCACAAGTGAGGAGGCCCTGAGAATTGCTATTATAAAAGGATGGTACAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 map04016 map04075
Pfam Domains
Protein Families
HLH bHLH-MYC_N

Protein Analysis

468

Amino Acids

52.54

Weight (kDa)

6.34

Isoelectric Point (pI)

42.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH-MYC_N PF14215 22 - 200 1.1e-38 bHLH-MYC and R2R3-MYB transcription factors N-terminal
HLH PF00010 292 - 338 8e-12 Helix-loop-helix DNA-binding domain
bHLH-TF_ACT-like_plant PF22754 391 - 461 7.4e-07 Plant bHLH transcription factor, ACT-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000212)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G00870
fragaria_vesca FvH4_5g10850 FvH4_5g10851 FvH4_5g23290 FvH4_7g17380
malus_domestica MD00G1137900.v1.1 MD01G1086800.v1.1 MD01G1086900.v1.1 MD03G1096500.v1.1 MD06G1119900.v1.1 MD06G1120000.v1.1 MD06G1120100.v1.1 MD06G1120200.v1.1 MD11G1120400.v1.1 MD11G1145000.v1.1 MD11G1254000.v1.1 MD11G1254100.v1.1 MD14G1126900.v1.1 MD14G1127000.v1.1 MD14G1137200.v1.1
prunus_persica Prupe.2G195300_v2.0.a1 Prupe.2G195300_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087900_v2.0.a1 Prupe.5G130300_v2.0.a1 Prupe.5G130400_v2.0.a1 Prupe.5G130500_v2.0.a1 Prupe.5G130500_v2.0.a1 Prupe.5G130600_v2.0.a1 Prupe.5G130700_v2.0.a1
pyrus_communis pycom01g09980 pycom06g11100 pycom06g11120 pycom06g11150 pycom11g10170 pycom11g22490 pycom14g11190 pycom14g11210
rosa_chinensis RchiOBHm_Chr1g0360811 RchiOBHm_Chr5g0055891 RchiOBHm_Chr6g0281801 RchiOBHm_Chr7g0187141 RchiOBHm_Chr7g0187261 RchiOBHm_Chr7g0212241 RchiOBHm_Chr7g0228701
rosa_laevigata RLG00000002910 RLG00000002911 RLG00000004796 RLG00000004799 RLG00000027784
rosa_multiflora Rmu_sc0000340.1_g000036 Rmu_sc0000378.1_g000013 Rmu_sc0000441.1_g000071 Rmu_sc0000559.1_g000036 Rmu_sc0000637.1_g000017 Rmu_sc0000765.1_g000001 Rmu_sc0000785.1_g000012 Rmu_sc0000854.1_g000018 Rmu_sc0000959.1_g000018 Rmu_sc0001018.1_g000022 Rmu_sc0001136.1_g000078 Rmu_sc0001433.1_g000037 Rmu_sc0001687.1_g000010 Rmu_sc0001716.1_g000014 Rmu_sc0001758.1_g000023 Rmu_sc0001758.1_g000031 Rmu_sc0001826.1_g000009 Rmu_sc0001850.1_g000027 Rmu_sc0001911.1_g000019 Rmu_sc0001937.1_g000001 Rmu_sc0002283.1_g000088 Rmu_sc0002532.1_g000058 Rmu_sc0002571.1_g000032 Rmu_sc0002888.1_g000014 Rmu_sc0002939.1_g000019 Rmu_sc0003197.1_g000007 Rmu_sc0003546.1_g000034 Rmu_sc0004002.1_g000003 Rmu_sc0004145.1_g000003 Rmu_sc0004301.1_g000013 Rmu_sc0004603.1_g000002 Rmu_sc0004748.1_g000007 Rmu_sc0004842.1_g000002 Rmu_sc0004990.1_g000012 Rmu_sc0005017.1_g000006 Rmu_sc0006343.1_g000001 Rmu_sc0006836.1_g000017 Rmu_sc0007491.1_g000003 Rmu_sc0008192.1_g000011 Rmu_sc0008877.1_g000007 Rmu_sc0010621.1_g000006 Rmu_sc0013354.1_g000017 Rmu_sc0013486.1_g000001 Rmu_sc0016511.1_g000001 Rmu_sc0021179.1_g000001 Rmu_sc0026563.1_g000001 Rmu_sc0027421.1_g000001 Rmu_sc0037114.1_g000001 Rmu_ssc0000022.1_g000039 Rmu_ssc0000050.1_g000048 Rmu_ssc0000141.1_g000004 Rmu_ssc0000280.1_g000002 Rmu_ssc0000387.1_g000024 Rmu_ssc0000459.1_g000030
rosa_roxburghii Rroxscaffold_3G00247010 Rroxscaffold_3G00267630 Rroxscaffold_3G00267670 Rroxscaffold_4G00295790 Rroxscaffold_4G00305930 Rroxscaffold_5G00346440 Rroxscaffold_7G00211970
rosa_rugosa Rorug01G0281800 Rorug02G0258400 Rorug04G0057400 Rorug04G0362000 Rorug04G0437300 Rorug06G0161400 Rorug06G0213300 Rorug06G0478800 Rorug06G0479300 Rorug06G0479300 Rorug07G0132200 Rorug07G0321200
rosa_samantha Rh1AG293700 Rh1BG258400 Rh1CG276200 Rh1DG288700 Rh7AG084400 Rh7AG084900 Rh7AG265100 Rh7BG078100 Rh7BG078600 Rh7CG085500 Rh7CG085800 Rh7CG281900 Rh7DG086500 Rh7DG086900 Rh7DG272300
rosa_wichuraiana Rw1G025990 Rw7G007440 Rw7G007460 Rw7G022700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1143, 1385
AarI CACCTGC 2 cut(s) 422, 1100
Acc36I ACCTGC 2 cut(s) 422, 1100
AccIII TCCGGA 1 cut(s) 1219
AciI CCGC 2 cut(s) 525, 668
AclWI GGATC 3 cut(s) 610, 737, 769
AcoI YGGCCR 1 cut(s) 172
AcsI RAATTY 2 cut(s) 492, 784
AcuI CTGAAG 1 cut(s) 431
AdeI CACNNNGTG 1 cut(s) 725
AfaI GTAC 5 cut(s) 400, 531, 660, 730, 1397
AfiI CCNNNNNNNGG 2 cut(s) 102, 883
AgsI TTSAA 5 cut(s) 65, 114, 275, 536, 1322
AjnI CCWGG 3 cut(s) 20, 1054, 1266
AluBI AGCT 8 cut(s) 278, 445, 634, 698, 910, 971, 1009, 1157
AluI AGCT 8 cut(s) 278, 445, 634, 698, 910, 971, 1009, 1157
Alw26I GTCTC 2 cut(s) 306, 628
AlwI GGATC 3 cut(s) 610, 737, 769
AlwNI CAGNNNCTG 1 cut(s) 698
Aor13HI TCCGGA 1 cut(s) 1219
AoxI GGCC 8 cut(s) 92, 145, 172, 198, 1035, 1081, 1121, 1365
ApeKI GCWGC 4 cut(s) 631, 639, 642, 1154
ApoI RAATTY 2 cut(s) 492, 784
ArsI GACNNNNNNTTYG 2 cut(s) 90, 122
Asp700I GAANNNNTTC 1 cut(s) 688
AspS9I GGNCC 3 cut(s) 93, 652, 1366
AsuHPI GGTGA 4 cut(s) 425, 446, 756, 781
AsuII TTCGAA 1 cut(s) 181
AvaII GGWCC 1 cut(s) 652
AxyI CCTNAGG 1 cut(s) 1344
BalI TGGCCA 1 cut(s) 174
BarI GAAGNNNNNNTAC 4 cut(s) 502, 534, 1188, 1220
BbsI GAAGAC 1 cut(s) 18
BbvI GCAGC 4 cut(s) 643, 651, 654, 1166
BccI CCATC 4 cut(s) 299, 775, 924, 1386
BceAI ACGGC 2 cut(s) 630, 1142
BciT130I CCWGG 3 cut(s) 22, 1056, 1268
BclI TGATCA 3 cut(s) 436, 555, 1105
BcoDI GTCTC 2 cut(s) 306, 628
BfaI CTAG 5 cut(s) 149, 189, 674, 1242, 1402
BfmI CTRYAG 2 cut(s) 71, 753
BfuAI ACCTGC 2 cut(s) 422, 1100
BisI GCNGC 4 cut(s) 632, 640, 643, 1155
BlsI GCNGC 4 cut(s) 633, 641, 644, 1156
BmcAI AGTACT 1 cut(s) 531
Bme1390I CCNGG 3 cut(s) 22, 1056, 1268
Bme18I GGWCC 1 cut(s) 652
BmgT120I GGNCC 3 cut(s) 93, 652, 1366
BmrFI CCNGG 3 cut(s) 22, 1056, 1268
BmrI ACTGGG 1 cut(s) 355
BmsI GCATC 2 cut(s) 976, 1305
BmuI ACTGGG 1 cut(s) 355
BpiI GAAGAC 1 cut(s) 18
BpmI CTGGAG 2 cut(s) 1052, 1289
Bpu14I TTCGAA 1 cut(s) 181
Bsa29I ATCGAT 1 cut(s) 919
BsaBI GATNNNNATC 2 cut(s) 766, 1110
BsaJI CCNNGG 2 cut(s) 379, 1124
BsaWI WCCGGW 1 cut(s) 1219
BsaXI ACNNNNNCTCC 2 cut(s) 185, 215
Bsc4I CCNNNNNNNGG 2 cut(s) 102, 883
Bse1I ACTGG 3 cut(s) 361, 856, 1035
Bse21I CCTNAGG 1 cut(s) 1344
Bse3DI GCAATG 1 cut(s) 148
Bse8I GATNNNNATC 2 cut(s) 766, 1110
BseAI TCCGGA 1 cut(s) 1219
BseBI CCWGG 3 cut(s) 22, 1056, 1268
BseCI ATCGAT 1 cut(s) 919
BseDI CCNNGG 2 cut(s) 379, 1124
BseGI GGATG 3 cut(s) 310, 767, 1397
BseJI GATNNNNATC 2 cut(s) 766, 1110
BseLI CCNNNNNNNGG 2 cut(s) 102, 883
BseMI GCAATG 1 cut(s) 148
BseMII CTCAG 5 cut(s) 318, 786, 1206, 1358, 1361
BseNI ACTGG 3 cut(s) 361, 856, 1035
BseRI GAGGAG 1 cut(s) 1376
BseXI GCAGC 4 cut(s) 643, 651, 654, 1166
BseYI CCCAGC 1 cut(s) 1157
BsgI GTGCAG 1 cut(s) 658
BshFI GGCC 8 cut(s) 94, 147, 174, 200, 1037, 1083, 1123, 1367
BshVI ATCGAT 1 cut(s) 919
BsiSI CCGG 1 cut(s) 1220
BslFI GGGAC 1 cut(s) 206
BslI CCNNNNNNNGG 2 cut(s) 102, 883
BsmAI GTCTC 2 cut(s) 306, 628
BsmBI CGTCTC 1 cut(s) 306
BsmFI GGGAC 1 cut(s) 206
BsmI GAATGC 2 cut(s) 489, 1258
BsnI GGCC 8 cut(s) 94, 147, 174, 200, 1037, 1083, 1123, 1367
Bsp119I TTCGAA 1 cut(s) 181
Bsp13I TCCGGA 1 cut(s) 1219
Bsp143I GATC 9 cut(s) 436, 555, 602, 742, 761, 833, 857, 1105, 1182
BspACI CCGC 2 cut(s) 525, 668
BspANI GGCC 8 cut(s) 94, 147, 174, 200, 1037, 1083, 1123, 1367
BspCNI CTCAG 5 cut(s) 319, 787, 1205, 1357, 1362
BspDI ATCGAT 1 cut(s) 919
BspEI TCCGGA 1 cut(s) 1219
BspHI TCATGA 1 cut(s) 439
BspMAI CTGCAG 1 cut(s) 75
BspMI ACCTGC 2 cut(s) 422, 1100
BspPI GGATC 3 cut(s) 610, 737, 769
BspT104I TTCGAA 1 cut(s) 181
BsrDI GCAATG 1 cut(s) 148
BsrI ACTGG 3 cut(s) 361, 856, 1035
BssECI CCNNGG 2 cut(s) 379, 1124
BssMI GATC 9 cut(s) 436, 555, 602, 742, 761, 833, 857, 1105, 1182
Bst2UI CCWGG 3 cut(s) 22, 1056, 1268
Bst4CI ACNGT 3 cut(s) 383, 620, 845
BstBI TTCGAA 1 cut(s) 181
BstC8I GCNNGC 4 cut(s) 126, 376, 429, 1291
BstDEI CTNAG 5 cut(s) 327, 795, 1192, 1344, 1370
BstDSI CCRYGG 2 cut(s) 379, 1124
BstF5I GGATG 3 cut(s) 310, 767, 1397
BstKTI GATC 9 cut(s) 439, 558, 605, 745, 764, 836, 860, 1108, 1185
BstMAI GTCTC 2 cut(s) 306, 628
BstMBI GATC 9 cut(s) 436, 555, 602, 742, 761, 833, 857, 1105, 1182
BstMWI GCNNNNNNNGC 2 cut(s) 907, 1160
BstNI CCWGG 3 cut(s) 22, 1056, 1268
BstSCI CCNGG 3 cut(s) 20, 1054, 1266
BstSFI CTRYAG 2 cut(s) 71, 753
BstV1I GCAGC 4 cut(s) 643, 651, 654, 1166
BstV2I GAAGAC 1 cut(s) 18
BstX2I RGATCY 1 cut(s) 742
BstYI RGATCY 1 cut(s) 742
Bsu15I ATCGAT 1 cut(s) 919
Bsu36I CCTNAGG 1 cut(s) 1344
BsuRI GGCC 8 cut(s) 94, 147, 174, 200, 1037, 1083, 1123, 1367
BsuTUI ATCGAT 1 cut(s) 919
BtgI CCRYGG 2 cut(s) 379, 1124
BtsCI GGATG 3 cut(s) 310, 767, 1397
BtsI GCAGTG 1 cut(s) 345
BtsIMutI CAGTG 4 cut(s) 345, 625, 850, 1220
BveI ACCTGC 2 cut(s) 422, 1100
Cac8I GCNNGC 4 cut(s) 126, 376, 429, 1291
CaiI CAGNNNCTG 1 cut(s) 698
CciI TCATGA 1 cut(s) 439
Cfr13I GGNCC 3 cut(s) 93, 652, 1366
ClaI ATCGAT 1 cut(s) 919
CseI GACGC 1 cut(s) 332
Csp6I GTAC 5 cut(s) 399, 530, 659, 729, 1396
CspCI CAANNNNNGTGG 2 cut(s) 692, 727
CviAII CATG 8 cut(s) 295, 440, 488, 522, 656, 878, 1201, 1286
CviQI GTAC 5 cut(s) 399, 530, 659, 729, 1396
DdeI CTNAG 5 cut(s) 327, 795, 1192, 1344, 1370
DpnI GATC 9 cut(s) 438, 557, 604, 744, 763, 835, 859, 1107, 1184
DpnII GATC 9 cut(s) 436, 555, 602, 742, 761, 833, 857, 1105, 1182
DraIII CACNNNGTG 1 cut(s) 725
EaeI YGGCCR 1 cut(s) 172
Eco47I GGWCC 1 cut(s) 652
Eco57I CTGAAG 1 cut(s) 431
Eco81I CCTNAGG 1 cut(s) 1344
EcoO109I RGGNCCY 1 cut(s) 1366
EcoRI GAATTC 1 cut(s) 492
EcoRII CCWGG 3 cut(s) 20, 1054, 1266
EcoT22I ATGCAT 1 cut(s) 489
Esp3I CGTCTC 1 cut(s) 306
FaeI CATG 8 cut(s) 298, 443, 491, 525, 659, 881, 1204, 1289
FaqI GGGAC 1 cut(s) 206
FatI CATG 8 cut(s) 294, 439, 487, 521, 655, 877, 1200, 1285
FbaI TGATCA 3 cut(s) 436, 555, 1105
Fnu4HI GCNGC 4 cut(s) 632, 640, 643, 1155
FokI GGATG 2 cut(s) 317, 754
Fsp4HI GCNGC 4 cut(s) 632, 640, 643, 1155
FspBI CTAG 5 cut(s) 149, 189, 674, 1242, 1402
GluI GCNGC 4 cut(s) 632, 640, 643, 1155
GsaI CCCAGC 1 cut(s) 1161
GsuI CTGGAG 2 cut(s) 1052, 1289
HaeIII GGCC 8 cut(s) 94, 147, 174, 200, 1037, 1083, 1123, 1367
HapII CCGG 1 cut(s) 1220
HgaI GACGC 1 cut(s) 332
Hin1II CATG 8 cut(s) 298, 443, 491, 525, 659, 881, 1204, 1289
HincII GTYRAC 1 cut(s) 1072
HindII GTYRAC 1 cut(s) 1072
HindIII AAGCTT 2 cut(s) 908, 969
HinfI GANTC 2 cut(s) 773, 921
HpaII CCGG 1 cut(s) 1220
HphI GGTGA 4 cut(s) 425, 446, 756, 781
Hpy166II GTNNAC 2 cut(s) 714, 1072
Hpy188I TCNGA 6 cut(s) 87, 772, 796, 862, 985, 1195
Hpy188III TCNNGA 6 cut(s) 44, 440, 693, 957, 1220, 1322
Hpy8I GTNNAC 2 cut(s) 714, 1072
Hpy99I CGWCG 2 cut(s) 326, 944
HpyAV CCTTC 2 cut(s) 247, 466
HpyCH4III ACNGT 3 cut(s) 383, 620, 845
HpyCH4IV ACGT 3 cut(s) 67, 315, 401
HpyCH4V TGCA 9 cut(s) 73, 487, 515, 639, 989, 995, 1027, 1256, 1289
HpyF10VI GCNNNNNNNGC 2 cut(s) 907, 1160
HpyF3I CTNAG 5 cut(s) 327, 795, 1192, 1344, 1370
HpySE526I ACGT 3 cut(s) 67, 315, 401
Hsp92II CATG 8 cut(s) 298, 443, 491, 525, 659, 881, 1204, 1289
Kpn2I TCCGGA 1 cut(s) 1219
Ksp22I TGATCA 3 cut(s) 436, 555, 1105
Kzo9I GATC 9 cut(s) 436, 555, 602, 742, 761, 833, 857, 1105, 1182
LmnI GCTCC 2 cut(s) 695, 1100
Lsp1109I GCAGC 4 cut(s) 643, 651, 654, 1166
LweI GCATC 2 cut(s) 976, 1305
MaeI CTAG 5 cut(s) 149, 189, 674, 1242, 1402
MaeII ACGT 3 cut(s) 67, 315, 401
MaeIII GTNAC 5 cut(s) 212, 316, 383, 748, 1272
MalI GATC 9 cut(s) 438, 557, 604, 744, 763, 835, 859, 1107, 1184
MboI GATC 9 cut(s) 436, 555, 602, 742, 761, 833, 857, 1105, 1182
MboII GAAGA 3 cut(s) 18, 262, 1192
MflI RGATCY 1 cut(s) 742
MlsI TGGCCA 1 cut(s) 174
MluCI AATT 7 cut(s) 13, 34, 81, 114, 492, 784, 1374
MluNI TGGCCA 1 cut(s) 174
MlyI GAGTC 1 cut(s) 767
MmeI TCCRAC 2 cut(s) 760, 795
Mox20I TGGCCA 1 cut(s) 174
Mph1103I ATGCAT 1 cut(s) 489
MroI TCCGGA 1 cut(s) 1219
MroXI GAANNNNTTC 1 cut(s) 688
MscI TGGCCA 1 cut(s) 174
MseI TTAA 2 cut(s) 356, 912
Msp20I TGGCCA 1 cut(s) 174
MspA1I CMGCKG 2 cut(s) 634, 1157
MspI CCGG 1 cut(s) 1220
MspR9I CCNGG 3 cut(s) 22, 1056, 1268
Mva1269I GAATGC 2 cut(s) 489, 1258
MvaI CCWGG 3 cut(s) 22, 1056, 1268
MwoI GCNNNNNNNGC 2 cut(s) 907, 1160
NdeII GATC 9 cut(s) 436, 555, 602, 742, 761, 833, 857, 1105, 1182
NlaIII CATG 8 cut(s) 298, 443, 491, 525, 659, 881, 1204, 1289
NmuCI GTSAC 1 cut(s) 748
NsiI ATGCAT 1 cut(s) 489
NspV TTCGAA 1 cut(s) 181
PagI TCATGA 1 cut(s) 439
PaqCI CACCTGC 2 cut(s) 422, 1100
PcsI WCGNNNNNNNCGW 1 cut(s) 546
PctI GAATGC 2 cut(s) 489, 1258
PdmI GAANNNNTTC 1 cut(s) 688
PfeI GAWTC 1 cut(s) 921
PkrI GCNGC 4 cut(s) 633, 641, 644, 1156
PleI GAGTC 1 cut(s) 767
PpsI GAGTC 1 cut(s) 767
PsiI TTATAA 2 cut(s) 1143, 1385
Psp6I CCWGG 3 cut(s) 20, 1054, 1266
PspFI CCCAGC 1 cut(s) 1157
PspGI CCWGG 3 cut(s) 20, 1054, 1266
PspPI GGNCC 3 cut(s) 93, 652, 1366
PsrI GAACNNNNNNTAC 2 cut(s) 391, 423
PstI CTGCAG 1 cut(s) 75
PstNI CAGNNNCTG 1 cut(s) 698
PsuI RGATCY 1 cut(s) 742
PvuII CAGCTG 2 cut(s) 634, 1157
RsaI GTAC 5 cut(s) 400, 531, 660, 730, 1397
RsaNI GTAC 5 cut(s) 399, 530, 659, 729, 1396
SaqAI TTAA 2 cut(s) 356, 912
SatI GCNGC 4 cut(s) 632, 640, 643, 1155
Sau3AI GATC 9 cut(s) 436, 555, 602, 742, 761, 833, 857, 1105, 1182
Sau96I GGNCC 3 cut(s) 93, 652, 1366
ScaI AGTACT 1 cut(s) 531
SchI GAGTC 1 cut(s) 767
ScrFI CCNGG 3 cut(s) 22, 1056, 1268
SfaNI GCATC 2 cut(s) 976, 1305
SfcI CTRYAG 2 cut(s) 71, 753
SfuI TTCGAA 1 cut(s) 181
SinI GGWCC 1 cut(s) 652
Sse9I AATT 7 cut(s) 13, 34, 81, 114, 492, 784, 1374
SsiI CCGC 2 cut(s) 525, 668
SspMI CTAG 5 cut(s) 149, 189, 674, 1242, 1402
StyD4I CCNGG 3 cut(s) 20, 1054, 1266
TaaI ACNGT 3 cut(s) 383, 620, 845
TaiI ACGT 3 cut(s) 70, 318, 404
TaqI TCGA 4 cut(s) 181, 234, 549, 919
TasI AATT 7 cut(s) 13, 34, 81, 114, 492, 784, 1374
TatI WGTACW 2 cut(s) 529, 658
TfiI GAWTC 1 cut(s) 921
Tru1I TTAA 2 cut(s) 356, 912
Tru9I TTAA 2 cut(s) 356, 912
TscAI CASTG 4 cut(s) 352, 625, 850, 1220
TseFI GTSAC 1 cut(s) 748
TseI GCWGC 4 cut(s) 631, 639, 642, 1154
Tsp45I GTSAC 1 cut(s) 748
TspDTI ATGAA 6 cut(s) 18, 67, 303, 510, 1181, 1265
TspGWI ACGGA 2 cut(s) 923, 928
TspRI CASTG 4 cut(s) 352, 625, 850, 1220
VpaK11BI GGWCC 1 cut(s) 652
XapI RAATTY 2 cut(s) 492, 784
XcmI CCANNNNNNNNNTGG 1 cut(s) 1052
XmnI GAANNNNTTC 1 cut(s) 688
XspI CTAG 5 cut(s) 149, 189, 674, 1242, 1402
ZrmI AGTACT 1 cut(s) 531
Zsp2I ATGCAT 1 cut(s) 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.