Rroxscaffold_3G00267630
BHLH Family

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000212
Physical Location & Seq
Reverse (-)
60730891 .. 60734025
3135 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00267630.1

Sequence Viewer

Length: 1371 bp
ATGGGTGAGATTATTTCCTCATGTTCTTCACCCTACAATGTTTATCCAGAAACTACCTCAGCCACATTGCTTCAACAACGCCTCCAATTCATACTTCAGAACCGCCCTGAAACCTGGCTTTATTCCATTTTCTGGAAAGCCTCCAAAGACAGTAACGACGACAACGGCGTTTCTTTGTCATGGGCCGGAGGCCATTTCAGAGGCATTAGAGACTTCTCATCCAAAAAATCAAGCATTCAGCACTCGGATAATAACTACCGAGACGTGAGGAGAGAGATCGAAGCCCTGTGTCATGACGACATGGACTCGGAGAGATTTGAAGACATTAACGGAGACGTGACTGACTCCGAGTGGTTCTACTTCTACACCGTCTCTTTAACACAGTCGTTTGCTGCAGGCCACAATGGGAATTCCAACATTCTGGGCCGTGTGCATTCTTCTAGTGCTTTTATTTGGTTGGCAGGAGATGGTGAGTTTCAGTTCTATGAGTGTGAGAGAGTTAAAGAGGCTCGCATGCATGGAATTCGAACTCTGGTTTGTGTTGCAACTTATAGTGGGGTGCTTGAAGTGGCTTCTTTGGATGTGATTAAAGAAGATTGGGGTTTTGTGCAGCAAGCAAAATCACTTTTTGGAATATCAGACTCCAACTCAAAGCATGGAAGCCAGGAGGCTCATGTTCACGTTCCTCTGCCCCAAAATGGATTAATGTTTTCAGCAACTCAAAAGCAGTCGAATACACATGGGCGTGGTAAGAGAGAAGTGGCTTTCAATATAGGTGGGTCGTCATCTGATTCAGGGCCTTCTGAAAATATAGAAAATAATCGATCAAAAAAGAGAGGGAGGTCATCAAGCCATGTGAACGGCAGACGAGAATCACCACCACTAAACCATGTGGAAGCAGAGAGACAACGACGTGAAAGGCTGAATCATCGATTCTATGCTCTGAGAGCTGCTGTTCCAAATGTGTCAAGGATGGACAAAGCTTCTTTACTTGCTGATGCAGTTGAGTACATCAATAATCTGAAGACAAAGATTGATAAACTGGAGGCCAAACTCCAAGCACAAACCAAGATACCCAAGGTGGGTAGCACCAACTCCATAAGTTACAGAGCATCTGCTGTTGTGGAAGTGGATGTGAAATTTGTAGGCTCTGAAGCAATGATTCGAGTTCGGAGTACGGATAATGAGGACTATCCATATGCAAGATTGATGAATGCACTCAAAGACCTCGAATTGCATATTTGTCATGCAAGCATTTCAAGTGTGAAGGAGTTTATGCTTCAAGATGTTGTGGCACGAGTTCCATTTGGATTCACAAGTGAGGAGGCCATGAGAACCGCCATTATAAAACGATTGTCAAACTTTCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 map04016 map04075
Pfam Domains
Protein Families
HLH bHLH-MYC_N

Protein Analysis

456

Amino Acids

51.28

Weight (kDa)

7.26

Isoelectric Point (pI)

51.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH-MYC_N PF14215 24 - 210 2.7e-39 bHLH-MYC and R2R3-MYB transcription factors N-terminal
HLH PF00010 296 - 342 1.3e-11 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000212)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G00870
fragaria_vesca FvH4_5g10850 FvH4_5g10851 FvH4_5g23290 FvH4_7g17380
malus_domestica MD00G1137900.v1.1 MD01G1086800.v1.1 MD01G1086900.v1.1 MD03G1096500.v1.1 MD06G1119900.v1.1 MD06G1120000.v1.1 MD06G1120100.v1.1 MD06G1120200.v1.1 MD11G1120400.v1.1 MD11G1145000.v1.1 MD11G1254000.v1.1 MD11G1254100.v1.1 MD14G1126900.v1.1 MD14G1127000.v1.1 MD14G1137200.v1.1
prunus_persica Prupe.2G195300_v2.0.a1 Prupe.2G195300_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087900_v2.0.a1 Prupe.5G130300_v2.0.a1 Prupe.5G130400_v2.0.a1 Prupe.5G130500_v2.0.a1 Prupe.5G130500_v2.0.a1 Prupe.5G130600_v2.0.a1 Prupe.5G130700_v2.0.a1
pyrus_communis pycom01g09980 pycom06g11100 pycom06g11120 pycom06g11150 pycom11g10170 pycom11g22490 pycom14g11190 pycom14g11210
rosa_chinensis RchiOBHm_Chr1g0360811 RchiOBHm_Chr5g0055891 RchiOBHm_Chr6g0281801 RchiOBHm_Chr7g0187141 RchiOBHm_Chr7g0187261 RchiOBHm_Chr7g0212241 RchiOBHm_Chr7g0228701
rosa_laevigata RLG00000002910 RLG00000002911 RLG00000004796 RLG00000004799 RLG00000027784
rosa_multiflora Rmu_sc0000340.1_g000036 Rmu_sc0000378.1_g000013 Rmu_sc0000441.1_g000071 Rmu_sc0000559.1_g000036 Rmu_sc0000637.1_g000017 Rmu_sc0000765.1_g000001 Rmu_sc0000785.1_g000012 Rmu_sc0000854.1_g000018 Rmu_sc0000959.1_g000018 Rmu_sc0001018.1_g000022 Rmu_sc0001136.1_g000078 Rmu_sc0001433.1_g000037 Rmu_sc0001687.1_g000010 Rmu_sc0001716.1_g000014 Rmu_sc0001758.1_g000023 Rmu_sc0001758.1_g000031 Rmu_sc0001826.1_g000009 Rmu_sc0001850.1_g000027 Rmu_sc0001911.1_g000019 Rmu_sc0001937.1_g000001 Rmu_sc0002283.1_g000088 Rmu_sc0002532.1_g000058 Rmu_sc0002571.1_g000032 Rmu_sc0002888.1_g000014 Rmu_sc0002939.1_g000019 Rmu_sc0003197.1_g000007 Rmu_sc0003546.1_g000034 Rmu_sc0004002.1_g000003 Rmu_sc0004145.1_g000003 Rmu_sc0004301.1_g000013 Rmu_sc0004603.1_g000002 Rmu_sc0004748.1_g000007 Rmu_sc0004842.1_g000002 Rmu_sc0004990.1_g000012 Rmu_sc0005017.1_g000006 Rmu_sc0006343.1_g000001 Rmu_sc0006836.1_g000017 Rmu_sc0007491.1_g000003 Rmu_sc0008192.1_g000011 Rmu_sc0008877.1_g000007 Rmu_sc0010621.1_g000006 Rmu_sc0013354.1_g000017 Rmu_sc0013486.1_g000001 Rmu_sc0016511.1_g000001 Rmu_sc0021179.1_g000001 Rmu_sc0026563.1_g000001 Rmu_sc0027421.1_g000001 Rmu_sc0037114.1_g000001 Rmu_ssc0000022.1_g000039 Rmu_ssc0000050.1_g000048 Rmu_ssc0000141.1_g000004 Rmu_ssc0000280.1_g000002 Rmu_ssc0000387.1_g000024 Rmu_ssc0000459.1_g000030
rosa_roxburghii Rroxscaffold_3G00247010 Rroxscaffold_3G00267630 Rroxscaffold_3G00267670 Rroxscaffold_4G00295790 Rroxscaffold_4G00305930 Rroxscaffold_5G00346440 Rroxscaffold_7G00211970
rosa_rugosa Rorug01G0281800 Rorug02G0258400 Rorug04G0057400 Rorug04G0362000 Rorug04G0437300 Rorug06G0161400 Rorug06G0213300 Rorug06G0478800 Rorug06G0479300 Rorug06G0479300 Rorug07G0132200 Rorug07G0321200
rosa_samantha Rh1AG293700 Rh1BG258400 Rh1CG276200 Rh1DG288700 Rh7AG084400 Rh7AG084900 Rh7AG265100 Rh7BG078100 Rh7BG078600 Rh7CG085500 Rh7CG085800 Rh7CG281900 Rh7DG086500 Rh7DG086900 Rh7DG272300
rosa_wichuraiana Rw1G025990 Rw7G007440 Rw7G007460 Rw7G022700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1346
AccB7I CCANNNNNTGG 1 cut(s) 132
AciI CCGC 2 cut(s) 103, 1338
AcsI RAATTY 3 cut(s) 409, 522, 1139
AcuI CTGAAG 3 cut(s) 80, 1043, 1173
AfaI GTAC 2 cut(s) 1010, 1177
AfiI CCNNNNNNNGG 3 cut(s) 132, 698, 1082
AgsI TTSAA 6 cut(s) 74, 320, 566, 769, 1260, 1283
AjiI CACGTC 3 cut(s) 265, 337, 914
AjnI CCWGG 2 cut(s) 113, 663
AjuI GAANNNNNNNTTGG 2 cut(s) 78, 110
AluBI AGCT 2 cut(s) 950, 983
AluI AGCT 2 cut(s) 950, 983
Alw26I GTCTC 5 cut(s) 204, 255, 327, 376, 898
AoxI GGCC 7 cut(s) 183, 190, 397, 424, 797, 1047, 1326
ApeKI GCWGC 3 cut(s) 392, 610, 950
ApoI RAATTY 3 cut(s) 409, 522, 1139
AseI ATTAAT 1 cut(s) 704
AspS9I GGNCC 3 cut(s) 183, 424, 797
AsuHPI GGTGA 4 cut(s) 17, 21, 482, 867
AsuII TTCGAA 1 cut(s) 526
BauI CACGAG 1 cut(s) 1296
BbsI GAAGAC 2 cut(s) 327, 1031
BbvCI CCTCAGC 1 cut(s) 58
BbvI GCAGC 3 cut(s) 379, 622, 937
BccI CCATC 2 cut(s) 461, 967
BceAI ACGGC 3 cut(s) 181, 411, 877
BcgI CGANNNNNNTGC 2 cut(s) 506, 540
BciT130I CCWGG 2 cut(s) 115, 665
BcoDI GTCTC 5 cut(s) 204, 255, 327, 376, 898
BfaI CTAG 2 cut(s) 441, 1369
BfmI CTRYAG 1 cut(s) 393
BisI GCNGC 3 cut(s) 393, 611, 951
BlsI GCNGC 3 cut(s) 394, 612, 952
Bme1390I CCNGG 2 cut(s) 115, 665
BmgBI CACGTC 3 cut(s) 265, 337, 914
BmgT120I GGNCC 3 cut(s) 183, 424, 797
BmrFI CCNGG 2 cut(s) 115, 665
BmsI GCATC 2 cut(s) 988, 1121
BpiI GAAGAC 2 cut(s) 327, 1031
BpmI CTGGAG 1 cut(s) 1064
Bpu10I CCTNAGC 1 cut(s) 58
Bpu14I TTCGAA 1 cut(s) 526
Bsa29I ATCGAT 2 cut(s) 823, 931
BsaJI CCNNGG 1 cut(s) 1077
BsaXI ACNNNNNCTCC 2 cut(s) 66, 96
Bsc4I CCNNNNNNNGG 3 cut(s) 132, 698, 1082
Bse1I ACTGG 1 cut(s) 1047
Bse3DI GCAATG 2 cut(s) 65, 1164
BseBI CCWGG 2 cut(s) 115, 665
BseCI ATCGAT 2 cut(s) 823, 931
BseDI CCNNGG 1 cut(s) 1077
BseGI GGATG 4 cut(s) 218, 586, 978, 1138
BseLI CCNNNNNNNGG 3 cut(s) 132, 698, 1082
BseMI GCAATG 2 cut(s) 65, 1164
BseMII CTCAG 2 cut(s) 72, 935
BseNI ACTGG 1 cut(s) 1047
BseRI GAGGAG 2 cut(s) 283, 1337
BseXI GCAGC 3 cut(s) 379, 622, 937
BsgI GTGCAG 1 cut(s) 629
BshFI GGCC 7 cut(s) 185, 192, 399, 426, 799, 1049, 1328
BshVI ATCGAT 2 cut(s) 823, 931
BsiSI CCGG 1 cut(s) 186
BslI CCNNNNNNNGG 3 cut(s) 132, 698, 1082
BsmAI GTCTC 5 cut(s) 204, 255, 327, 376, 898
BsmBI CGTCTC 3 cut(s) 255, 327, 376
BsmI GAATGC 3 cut(s) 234, 433, 1219
BsnI GGCC 7 cut(s) 185, 192, 399, 426, 799, 1049, 1328
Bsp119I TTCGAA 1 cut(s) 526
Bsp143I GATC 2 cut(s) 276, 824
BspACI CCGC 2 cut(s) 103, 1338
BspANI GGCC 7 cut(s) 185, 192, 399, 426, 799, 1049, 1328
BspCNI CTCAG 2 cut(s) 71, 936
BspDI ATCGAT 2 cut(s) 823, 931
BspHI TCATGA 1 cut(s) 292
BspMAI CTGCAG 1 cut(s) 397
BspT104I TTCGAA 1 cut(s) 526
BsrDI GCAATG 2 cut(s) 65, 1164
BsrI ACTGG 1 cut(s) 1047
BssECI CCNNGG 1 cut(s) 1077
BssMI GATC 2 cut(s) 276, 824
BssSI CACGAG 1 cut(s) 1296
BssT1I CCWWGG 1 cut(s) 1077
Bst2BI CACGAG 1 cut(s) 1296
Bst2UI CCWGG 2 cut(s) 115, 665
Bst4CI ACNGT 3 cut(s) 152, 370, 384
BstBI TTCGAA 1 cut(s) 526
BstC8I GCNNGC 5 cut(s) 397, 511, 515, 615, 1252
BstDEI CTNAG 2 cut(s) 58, 944
BstF5I GGATG 4 cut(s) 218, 586, 978, 1138
BstKTI GATC 2 cut(s) 279, 827
BstMAI GTCTC 5 cut(s) 204, 255, 327, 376, 898
BstMBI GATC 2 cut(s) 276, 824
BstMWI GCNNNNNNNGC 1 cut(s) 947
BstNI CCWGG 2 cut(s) 115, 665
BstNSI RCATGY 1 cut(s) 517
BstSCI CCNGG 2 cut(s) 113, 663
BstSFI CTRYAG 1 cut(s) 393
BstV1I GCAGC 3 cut(s) 379, 622, 937
BstV2I GAAGAC 2 cut(s) 327, 1031
BstXI CCANNNNNNTGG 1 cut(s) 421
Bsu15I ATCGAT 2 cut(s) 823, 931
BsuRI GGCC 7 cut(s) 185, 192, 399, 426, 799, 1049, 1328
BsuTUI ATCGAT 2 cut(s) 823, 931
BtrI CACGTC 3 cut(s) 265, 337, 914
BtsCI GGATG 4 cut(s) 218, 586, 978, 1138
Cac8I GCNNGC 5 cut(s) 397, 511, 515, 615, 1252
CciI TCATGA 1 cut(s) 292
Cfr13I GGNCC 3 cut(s) 183, 424, 797
ClaI ATCGAT 2 cut(s) 823, 931
Csp6I GTAC 2 cut(s) 1009, 1176
CspCI CAANNNNNGTGG 2 cut(s) 757, 792
CviQI GTAC 2 cut(s) 1009, 1176
DdeI CTNAG 2 cut(s) 58, 944
DpnI GATC 2 cut(s) 278, 826
DpnII GATC 2 cut(s) 276, 824
Eco130I CCWWGG 1 cut(s) 1077
Eco57I CTGAAG 3 cut(s) 80, 1043, 1173
EcoO109I RGGNCCY 1 cut(s) 797
EcoRI GAATTC 2 cut(s) 409, 522
EcoRII CCWGG 2 cut(s) 113, 663
EcoT14I CCWWGG 1 cut(s) 1077
EcoT22I ATGCAT 1 cut(s) 519
ErhI CCWWGG 1 cut(s) 1077
Esp3I CGTCTC 3 cut(s) 255, 327, 376
FauNDI CATATG 1 cut(s) 1198
Fnu4HI GCNGC 3 cut(s) 393, 611, 951
FokI GGATG 4 cut(s) 205, 593, 985, 1145
Fsp4HI GCNGC 3 cut(s) 393, 611, 951
FspBI CTAG 2 cut(s) 441, 1369
GluI GCNGC 3 cut(s) 393, 611, 951
GsuI CTGGAG 1 cut(s) 1064
HaeIII GGCC 7 cut(s) 185, 192, 399, 426, 799, 1049, 1328
HapII CCGG 1 cut(s) 186
HindIII AAGCTT 1 cut(s) 981
HinfI GANTC 9 cut(s) 305, 344, 641, 791, 872, 925, 933, 1162, 1311
HpaII CCGG 1 cut(s) 186
HphI GGTGA 4 cut(s) 17, 21, 482, 867
Hpy166II GTNNAC 2 cut(s) 679, 859
Hpy188III TCNNGA 4 cut(s) 47, 133, 293, 1283
Hpy8I GTNNAC 2 cut(s) 679, 859
Hpy99I CGWCG 2 cut(s) 161, 915
HpyAV CCTTC 2 cut(s) 810, 1261
HpyCH4III ACNGT 3 cut(s) 152, 370, 384
HpyCH4IV ACGT 4 cut(s) 264, 336, 681, 913
HpyF10VI GCNNNNNNNGC 1 cut(s) 947
HpyF3I CTNAG 2 cut(s) 58, 944
HpySE526I ACGT 4 cut(s) 264, 336, 681, 913
Kzo9I GATC 2 cut(s) 276, 824
Lsp1109I GCAGC 3 cut(s) 379, 622, 937
LweI GCATC 2 cut(s) 988, 1121
MaeI CTAG 2 cut(s) 441, 1369
MaeII ACGT 4 cut(s) 264, 336, 681, 913
MaeIII GTNAC 3 cut(s) 152, 337, 1103
MalI GATC 2 cut(s) 278, 826
MboI GATC 2 cut(s) 276, 824
MboII GAAGA 5 cut(s) 18, 332, 429, 605, 1036
MluCI AATT 5 cut(s) 86, 409, 522, 1139, 1232
MlyI GAGTC 3 cut(s) 299, 338, 635
MmeI TCCRAC 2 cut(s) 438, 669
Mph1103I ATGCAT 1 cut(s) 519
MseI TTAA 5 cut(s) 327, 377, 501, 588, 704
MslI CAYNNNNRTG 1 cut(s) 744
MspI CCGG 1 cut(s) 186
MspR9I CCNGG 2 cut(s) 115, 665
Mva1269I GAATGC 3 cut(s) 234, 433, 1219
MvaI CCWGG 2 cut(s) 115, 665
MwoI GCNNNNNNNGC 1 cut(s) 947
NdeI CATATG 1 cut(s) 1198
NdeII GATC 2 cut(s) 276, 824
NmuCI GTSAC 1 cut(s) 337
NsiI ATGCAT 1 cut(s) 519
NspI RCATGY 1 cut(s) 517
NspV TTCGAA 1 cut(s) 526
PaeI GCATGC 1 cut(s) 517
PagI TCATGA 1 cut(s) 292
PcsI WCGNNNNNNNCGW 1 cut(s) 165
PctI GAATGC 3 cut(s) 234, 433, 1219
PfeI GAWTC 6 cut(s) 791, 872, 925, 933, 1162, 1311
PflMI CCANNNNNTGG 1 cut(s) 132
PkrI GCNGC 3 cut(s) 394, 612, 952
PleI GAGTC 3 cut(s) 299, 338, 635
PpsI GAGTC 3 cut(s) 299, 338, 635
PshBI ATTAAT 1 cut(s) 704
PsiI TTATAA 1 cut(s) 1346
Psp6I CCWGG 2 cut(s) 113, 663
PspGI CCWGG 2 cut(s) 113, 663
PspPI GGNCC 3 cut(s) 183, 424, 797
PstI CTGCAG 1 cut(s) 397
RsaI GTAC 2 cut(s) 1010, 1177
RsaNI GTAC 2 cut(s) 1009, 1176
RseI CAYNNNNRTG 1 cut(s) 744
SaqAI TTAA 5 cut(s) 327, 377, 501, 588, 704
SatI GCNGC 3 cut(s) 393, 611, 951
Sau3AI GATC 2 cut(s) 276, 824
Sau96I GGNCC 3 cut(s) 183, 424, 797
SchI GAGTC 3 cut(s) 299, 338, 635
ScrFI CCNGG 2 cut(s) 115, 665
SfaNI GCATC 2 cut(s) 988, 1121
SfcI CTRYAG 1 cut(s) 393
SfuI TTCGAA 1 cut(s) 526
SmiMI CAYNNNNRTG 1 cut(s) 744
SphI GCATGC 1 cut(s) 517
Sse9I AATT 5 cut(s) 86, 409, 522, 1139, 1232
SsiI CCGC 2 cut(s) 103, 1338
SspMI CTAG 2 cut(s) 441, 1369
StyD4I CCNGG 2 cut(s) 113, 663
StyI CCWWGG 1 cut(s) 1077
TaaI ACNGT 3 cut(s) 152, 370, 384
TaiI ACGT 4 cut(s) 267, 339, 684, 916
TaqI TCGA 7 cut(s) 279, 526, 731, 823, 931, 1165, 1230
TasI AATT 5 cut(s) 86, 409, 522, 1139, 1232
TatI WGTACW 1 cut(s) 1008
TfiI GAWTC 6 cut(s) 791, 872, 925, 933, 1162, 1311
Tru1I TTAA 5 cut(s) 327, 377, 501, 588, 704
Tru9I TTAA 5 cut(s) 327, 377, 501, 588, 704
TseFI GTSAC 1 cut(s) 337
TseI GCWGC 3 cut(s) 392, 610, 950
Tsp45I GTSAC 1 cut(s) 337
TspDTI ATGAA 2 cut(s) 79, 1226
TspGWI ACGGA 2 cut(s) 345, 1193
Van91I CCANNNNNTGG 1 cut(s) 132
VspI ATTAAT 1 cut(s) 704
XapI RAATTY 3 cut(s) 409, 522, 1139
XceI RCATGY 1 cut(s) 517
XspI CTAG 2 cut(s) 441, 1369
Zsp2I ATGCAT 1 cut(s) 519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.