MD06G1120000.v1.1
BHLH Family

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0000212
Physical Location & Seq
Forward (+)
25897420 .. 25899498
2079 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1120000.v1.1.491

Sequence Viewer

Length: 1503 bp
ATGCCTTTTGATTCCATAAACTCCTCCCATACCATCGAGGCTTCTGAAAAAGAAAAAAAGGAAAAAAGGAAAACCATGGAGGAAATTATATCCTCTTGTACTTCTCAAAAGTTTTGTCAAGAAAACTCACCCACACTTCAACGCCTCCAGTTCATAGTTCAGAACCGCCCTGAATGGTGGGTCTATTCAATTTTCTGGCAACCCTCAAAACACGACAGTGACCTAGTTTCTTTGTCGTGGGCCGGCGGCCATTTTCGAAGCACTAGGGACTTGGCCTCCAAAAGAAGTTACCACAAACACCAACCCGGATTCGGGTTCAATGTTGTAGAGAAGAAGAGGGCGATTAGCAGAGAAGTTGAAGCTCTTTTCCATGAAGACGTGGATTTGGACGGTGGAGATGTTACTGACGCTCAATGGTTTTACTTTTACACAGCCTCTTTAACCCAGTCATTTACTGCCGGCCACGGTAATAGTAACATTCTGGGCCGTACGTTTTGTTCTGGTGGTTTTGTTTGGCTGGCAGGTGATCATGAGCTTCAATTCTATGAGTGTGAGAGAGTGAAAGAAGCAAGAATGCACGGAATTCAAACTTTGGTTTGTATTGCAACTTCATGTGGAGTACTTGAACTCGCATCTTTGGATGTGATCAAAGAAGATTGGGGTCTTCTGCAGCTATCAAAATCACTTTTCGAATCAGACAACAAGAACGTGGTCCCAAAGCAGCTGAGCAGCAGCCGTGAGGACCAGGTACATGTTTCTCTCTTAGAAAGTGGAATGTTTTCAGGAGCTGATAAAGAGTGGGCAACACAAGGTCGTACAAAAGAAGCAGCTCCTGCAAATGTAAGTGGATCGTCATCAGACTCAGGACCTTCTGACTCTGTTGGAAATTTAACCTCTGAGAATACTGAGAACATTCGATTAAAAAAGAGAGGAAGATCATCAAACCGTGAAACAGGTAGAACAGAATCACCAATAAACCATGTTGAGGCTGAAAGACAAAGGCGAGAGAAGCTTAACCATCGATTCTATGCCCTCCGCTCTGTCGTTCCCAATGTGTCCAGAATGGACAAAGCTTCTTTACTTTCTGATGCAGTTGTATACATCAATGATCTGAAAGCAAGGATTGGGCAATTGGAGGCCAAAATTCACGCACAACCTCAAAAGCCTGGTGTTGATATTGGAGGCCAAACAAGCTCCATGGTTGATCATCATCAAACAAGGCCTAGGCTATCATCAAGTTATAATAATAGCGCAGCTGGGGCTGTGGAAATAGATGTGAAGATCGTAGGCTCAGAAGCCATGATACGAGTTCAGTGTCCGGATTGCAATTACCCTAATGCTAGATTGATGAATGCACTCAAAGACCTGGAGTTACAAGTTTATCATGCAAGCATATCAAGTGTGAAAGAGTTGATGCTTCAAGATGTTGTGGCAAGAGTGCCTAAGGGTTTCACAAGTGATGAGGCCATGAGAATTGCTATTCTAAAAAGATGGTACAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 map04016 map04075
Pfam Domains
Protein Families
HLH bHLH-MYC_N

Protein Analysis

501

Amino Acids

56.09

Weight (kDa)

6.98

Isoelectric Point (pI)

51.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH-MYC_N PF14215 47 - 230 2.2e-39 bHLH-MYC and R2R3-MYB transcription factors N-terminal
HLH PF00010 326 - 372 3.9e-11 Helix-loop-helix DNA-binding domain
bHLH-TF_ACT-like_plant PF22754 423 - 495 1.1e-06 Plant bHLH transcription factor, ACT-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000212)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G00870
fragaria_vesca FvH4_5g10850 FvH4_5g10851 FvH4_5g23290 FvH4_7g17380
malus_domestica MD00G1137900.v1.1 MD01G1086800.v1.1 MD01G1086900.v1.1 MD03G1096500.v1.1 MD06G1119900.v1.1 MD06G1120000.v1.1 MD06G1120100.v1.1 MD06G1120200.v1.1 MD11G1120400.v1.1 MD11G1145000.v1.1 MD11G1254000.v1.1 MD11G1254100.v1.1 MD14G1126900.v1.1 MD14G1127000.v1.1 MD14G1137200.v1.1
prunus_persica Prupe.2G195300_v2.0.a1 Prupe.2G195300_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087900_v2.0.a1 Prupe.5G130300_v2.0.a1 Prupe.5G130400_v2.0.a1 Prupe.5G130500_v2.0.a1 Prupe.5G130500_v2.0.a1 Prupe.5G130600_v2.0.a1 Prupe.5G130700_v2.0.a1
pyrus_communis pycom01g09980 pycom06g11100 pycom06g11120 pycom06g11150 pycom11g10170 pycom11g22490 pycom14g11190 pycom14g11210
rosa_chinensis RchiOBHm_Chr1g0360811 RchiOBHm_Chr5g0055891 RchiOBHm_Chr6g0281801 RchiOBHm_Chr7g0187141 RchiOBHm_Chr7g0187261 RchiOBHm_Chr7g0212241 RchiOBHm_Chr7g0228701
rosa_laevigata RLG00000002910 RLG00000002911 RLG00000004796 RLG00000004799 RLG00000027784
rosa_multiflora Rmu_sc0000340.1_g000036 Rmu_sc0000378.1_g000013 Rmu_sc0000441.1_g000071 Rmu_sc0000559.1_g000036 Rmu_sc0000637.1_g000017 Rmu_sc0000765.1_g000001 Rmu_sc0000785.1_g000012 Rmu_sc0000854.1_g000018 Rmu_sc0000959.1_g000018 Rmu_sc0001018.1_g000022 Rmu_sc0001136.1_g000078 Rmu_sc0001433.1_g000037 Rmu_sc0001687.1_g000010 Rmu_sc0001716.1_g000014 Rmu_sc0001758.1_g000023 Rmu_sc0001758.1_g000031 Rmu_sc0001826.1_g000009 Rmu_sc0001850.1_g000027 Rmu_sc0001911.1_g000019 Rmu_sc0001937.1_g000001 Rmu_sc0002283.1_g000088 Rmu_sc0002532.1_g000058 Rmu_sc0002571.1_g000032 Rmu_sc0002888.1_g000014 Rmu_sc0002939.1_g000019 Rmu_sc0003197.1_g000007 Rmu_sc0003546.1_g000034 Rmu_sc0004002.1_g000003 Rmu_sc0004145.1_g000003 Rmu_sc0004301.1_g000013 Rmu_sc0004603.1_g000002 Rmu_sc0004748.1_g000007 Rmu_sc0004842.1_g000002 Rmu_sc0004990.1_g000012 Rmu_sc0005017.1_g000006 Rmu_sc0006343.1_g000001 Rmu_sc0006836.1_g000017 Rmu_sc0007491.1_g000003 Rmu_sc0008192.1_g000011 Rmu_sc0008877.1_g000007 Rmu_sc0010621.1_g000006 Rmu_sc0013354.1_g000017 Rmu_sc0013486.1_g000001 Rmu_sc0016511.1_g000001 Rmu_sc0021179.1_g000001 Rmu_sc0026563.1_g000001 Rmu_sc0027421.1_g000001 Rmu_sc0037114.1_g000001 Rmu_ssc0000022.1_g000039 Rmu_ssc0000050.1_g000048 Rmu_ssc0000141.1_g000004 Rmu_ssc0000280.1_g000002 Rmu_ssc0000387.1_g000024 Rmu_ssc0000459.1_g000030
rosa_roxburghii Rroxscaffold_3G00247010 Rroxscaffold_3G00267630 Rroxscaffold_3G00267670 Rroxscaffold_4G00295790 Rroxscaffold_4G00305930 Rroxscaffold_5G00346440 Rroxscaffold_7G00211970
rosa_rugosa Rorug01G0281800 Rorug02G0258400 Rorug04G0057400 Rorug04G0362000 Rorug04G0437300 Rorug06G0161400 Rorug06G0213300 Rorug06G0478800 Rorug06G0479300 Rorug06G0479300 Rorug07G0132200 Rorug07G0321200
rosa_samantha Rh1AG293700 Rh1BG258400 Rh1CG276200 Rh1DG288700 Rh7AG084400 Rh7AG084900 Rh7AG265100 Rh7BG078100 Rh7BG078600 Rh7CG085500 Rh7CG085800 Rh7CG281900 Rh7DG086500 Rh7DG086900 Rh7DG272300
rosa_wichuraiana Rw1G025990 Rw7G007440 Rw7G007460 Rw7G022700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1242
AarI CACCTGC 1 cut(s) 512
Acc36I ACCTGC 1 cut(s) 512
AccBSI CCGCTC 1 cut(s) 1038
AccI GTMKAC 1 cut(s) 1098
AccIII TCCGGA 1 cut(s) 1318
AciI CCGC 3 cut(s) 166, 246, 1036
AclWI GGATC 1 cut(s) 856
AcoI YGGCCR 2 cut(s) 247, 460
AcsI RAATTY 3 cut(s) 582, 886, 1143
AfaI GTAC 6 cut(s) 100, 490, 621, 750, 817, 1496
AfiI CCNNNNNNNGG 3 cut(s) 311, 312, 985
AflIII ACRYGT 1 cut(s) 751
AgsI TTSAA 8 cut(s) 140, 189, 319, 359, 539, 587, 626, 1421
AjiI CACGTC 1 cut(s) 379
AjnI CCWGG 3 cut(s) 744, 1165, 1365
AleI CACNNNNGTG 1 cut(s) 216
AlwI GGATC 1 cut(s) 856
AlwNI CAGNNNCTG 2 cut(s) 788, 833
Aor13HI TCCGGA 1 cut(s) 1318
AoxI GGCC 9 cut(s) 240, 247, 273, 460, 484, 1137, 1183, 1220, 1464
ApeKI GCWGC 6 cut(s) 670, 721, 729, 732, 827, 1253
ApoI RAATTY 3 cut(s) 582, 886, 1143
ArsI GACNNNNNNTTYG 2 cut(s) 100, 132
Asp700I GAANNNNTTC 1 cut(s) 778
AspA2I CCTAGG 1 cut(s) 1223
AspLEI GCGC 1 cut(s) 1253
AspS9I GGNCC 5 cut(s) 240, 484, 712, 742, 866
AsuC2I CCSGG 1 cut(s) 306
AsuHPI GGTGA 3 cut(s) 120, 536, 960
AsuII TTCGAA 2 cut(s) 256, 690
AvaII GGWCC 3 cut(s) 712, 742, 866
AvrII CCTAGG 1 cut(s) 1223
AxyI CCTNAGG 1 cut(s) 1443
BarI GAAGNNNNNNTAC 4 cut(s) 592, 624, 1287, 1319
BbsI GAAGAC 2 cut(s) 381, 656
BbvI GCAGC 6 cut(s) 682, 733, 741, 744, 839, 1265
BccI CCATC 3 cut(s) 41, 1026, 1485
BceAI ACGGC 2 cut(s) 471, 720
BciT130I CCWGG 3 cut(s) 746, 1167, 1367
BclI TGATCA 3 cut(s) 526, 645, 1204
BcnI CCSGG 1 cut(s) 306
BfaI CTAG 5 cut(s) 224, 264, 1224, 1341, 1501
BfmI CTRYAG 1 cut(s) 668
BfuAI ACCTGC 1 cut(s) 512
BisI GCNGC 7 cut(s) 247, 671, 722, 730, 733, 828, 1254
BlnI CCTAGG 1 cut(s) 1223
BlpI GCTNAGC 1 cut(s) 725
BlsI GCNGC 7 cut(s) 248, 672, 723, 731, 734, 829, 1255
BmcAI AGTACT 1 cut(s) 621
Bme1390I CCNGG 4 cut(s) 306, 746, 1167, 1367
Bme18I GGWCC 3 cut(s) 712, 742, 866
BmgBI CACGTC 1 cut(s) 379
BmgT120I GGNCC 5 cut(s) 240, 484, 712, 742, 866
BmiI GGNNCC 1 cut(s) 714
BmrFI CCNGG 4 cut(s) 306, 746, 1167, 1367
BmrI ACTGGG 1 cut(s) 439
BmsI GCATC 3 cut(s) 641, 1078, 1404
BmuI ACTGGG 1 cut(s) 439
BpiI GAAGAC 2 cut(s) 381, 656
BpmI CTGGAG 2 cut(s) 131, 1388
Bpu1102I GCTNAGC 1 cut(s) 725
Bpu14I TTCGAA 2 cut(s) 256, 690
BpuMI CCSGG 1 cut(s) 306
Bsa29I ATCGAT 1 cut(s) 1021
BsaBI GATNNNNATC 2 cut(s) 853, 1209
BsaJI CCNNGG 4 cut(s) 75, 463, 1197, 1223
BsaWI WCCGGW 1 cut(s) 1318
BsaXI ACNNNNNCTCC 2 cut(s) 260, 290
Bsc4I CCNNNNNNNGG 3 cut(s) 311, 312, 985
Bse118I RCCGGY 2 cut(s) 242, 458
Bse1I ACTGG 2 cut(s) 148, 445
Bse21I CCTNAGG 1 cut(s) 1443
Bse8I GATNNNNATC 2 cut(s) 853, 1209
BseAI TCCGGA 1 cut(s) 1318
BseBI CCWGG 3 cut(s) 746, 1167, 1367
BseCI ATCGAT 1 cut(s) 1021
BseDI CCNNGG 4 cut(s) 75, 463, 1197, 1223
BseGI GGATG 1 cut(s) 646
BseJI GATNNNNATC 2 cut(s) 853, 1209
BseLI CCNNNNNNNGG 3 cut(s) 311, 312, 985
BseMII CTCAG 5 cut(s) 716, 876, 888, 897, 1305
BseNI ACTGG 2 cut(s) 148, 445
BseRI GAGGAG 1 cut(s) 13
BseXI GCAGC 6 cut(s) 682, 733, 741, 744, 839, 1265
BseYI CCCAGC 1 cut(s) 1256
BshFI GGCC 9 cut(s) 242, 249, 275, 462, 486, 1139, 1185, 1222, 1466
BshVI ATCGAT 1 cut(s) 1021
BsiSI CCGG 4 cut(s) 243, 306, 459, 1319
BsiWI CGTACG 1 cut(s) 488
BslFI GGGAC 2 cut(s) 281, 698
BslI CCNNNNNNNGG 3 cut(s) 311, 312, 985
BsmFI GGGAC 2 cut(s) 281, 698
BsmI GAATGC 2 cut(s) 579, 1357
BsnI GGCC 9 cut(s) 242, 249, 275, 462, 486, 1139, 1185, 1222, 1466
Bsp119I TTCGAA 2 cut(s) 256, 690
Bsp13I TCCGGA 1 cut(s) 1318
Bsp143I GATC 7 cut(s) 526, 645, 848, 935, 1108, 1204, 1281
Bsp1720I GCTNAGC 1 cut(s) 725
Bsp19I CCATGG 2 cut(s) 75, 1197
BspACI CCGC 3 cut(s) 166, 246, 1036
BspANI GGCC 9 cut(s) 242, 249, 275, 462, 486, 1139, 1185, 1222, 1466
BspCNI CTCAG 5 cut(s) 717, 875, 889, 898, 1304
BspDI ATCGAT 1 cut(s) 1021
BspEI TCCGGA 1 cut(s) 1318
BspHI TCATGA 1 cut(s) 529
BspLI GGNNCC 1 cut(s) 714
BspMAI CTGCAG 1 cut(s) 672
BspMI ACCTGC 1 cut(s) 512
BspPI GGATC 1 cut(s) 856
BspT104I TTCGAA 2 cut(s) 256, 690
BsrBI CCGCTC 1 cut(s) 1038
BsrFI RCCGGY 2 cut(s) 242, 458
BsrI ACTGG 2 cut(s) 148, 445
BssAI RCCGGY 2 cut(s) 242, 458
BssECI CCNNGG 4 cut(s) 75, 463, 1197, 1223
BssMI GATC 7 cut(s) 526, 645, 848, 935, 1108, 1204, 1281
BssNAI GTATAC 1 cut(s) 1099
BssT1I CCWWGG 3 cut(s) 75, 1197, 1223
Bst1107I GTATAC 1 cut(s) 1099
Bst2UI CCWGG 3 cut(s) 746, 1167, 1367
Bst4CI ACNGT 4 cut(s) 218, 392, 467, 947
Bst6I CTCTTC 1 cut(s) 329
BstAPI GCANNNNNTGC 1 cut(s) 833
BstBI TTCGAA 2 cut(s) 256, 690
BstC8I GCNNGC 4 cut(s) 244, 460, 519, 1390
BstDEI CTNAG 7 cut(s) 725, 763, 862, 897, 906, 1291, 1443
BstDSI CCRYGG 3 cut(s) 75, 463, 1197
BstF5I GGATG 1 cut(s) 646
BstHHI GCGC 1 cut(s) 1253
BstKTI GATC 7 cut(s) 529, 648, 851, 938, 1111, 1207, 1284
BstMBI GATC 7 cut(s) 526, 645, 848, 935, 1108, 1204, 1281
BstMWI GCNNNNNNNGC 4 cut(s) 833, 1009, 1191, 1259
BstNI CCWGG 3 cut(s) 746, 1167, 1367
BstNSI RCATGY 1 cut(s) 755
BstSCI CCNGG 4 cut(s) 304, 744, 1165, 1365
BstSFI CTRYAG 1 cut(s) 668
BstV1I GCAGC 6 cut(s) 682, 733, 741, 744, 839, 1265
BstV2I GAAGAC 2 cut(s) 381, 656
BstZ17I GTATAC 1 cut(s) 1099
Bsu15I ATCGAT 1 cut(s) 1021
Bsu36I CCTNAGG 1 cut(s) 1443
BsuRI GGCC 9 cut(s) 242, 249, 275, 462, 486, 1139, 1185, 1222, 1466
BsuTUI ATCGAT 1 cut(s) 1021
BtgI CCRYGG 3 cut(s) 75, 463, 1197
BtrI CACGTC 1 cut(s) 379
BtsCI GGATG 1 cut(s) 646
BtsIMutI CAGTG 2 cut(s) 223, 1319
BveI ACCTGC 1 cut(s) 512
Cac8I GCNNGC 4 cut(s) 244, 460, 519, 1390
CaiI CAGNNNCTG 2 cut(s) 788, 833
CciI TCATGA 1 cut(s) 529
CfoI GCGC 1 cut(s) 1253
Cfr10I RCCGGY 2 cut(s) 242, 458
Cfr13I GGNCC 5 cut(s) 240, 484, 712, 742, 866
ClaI ATCGAT 1 cut(s) 1021
CseI GACGC 1 cut(s) 416
CsiI ACCWGGT 1 cut(s) 744
Csp6I GTAC 6 cut(s) 99, 489, 620, 749, 816, 1495
CviQI GTAC 6 cut(s) 99, 489, 620, 749, 816, 1495
DdeI CTNAG 7 cut(s) 725, 763, 862, 897, 906, 1291, 1443
DpnI GATC 7 cut(s) 528, 647, 850, 937, 1110, 1206, 1283
DpnII GATC 7 cut(s) 526, 645, 848, 935, 1108, 1204, 1281
EaeI YGGCCR 2 cut(s) 247, 460
Eam1104I CTCTTC 1 cut(s) 329
EarI CTCTTC 1 cut(s) 329
Eco130I CCWWGG 3 cut(s) 75, 1197, 1223
Eco147I AGGCCT 1 cut(s) 1222
Eco47I GGWCC 3 cut(s) 712, 742, 866
Eco81I CCTNAGG 1 cut(s) 1443
EcoO109I RGGNCCY 1 cut(s) 866
EcoRI GAATTC 1 cut(s) 582
EcoRII CCWGG 3 cut(s) 744, 1165, 1365
EcoT14I CCWWGG 3 cut(s) 75, 1197, 1223
ErhI CCWWGG 3 cut(s) 75, 1197, 1223
FaqI GGGAC 2 cut(s) 281, 698
FbaI TGATCA 3 cut(s) 526, 645, 1204
FblI GTMKAC 1 cut(s) 1098
Fnu4HI GCNGC 7 cut(s) 247, 671, 722, 730, 733, 828, 1254
FokI GGATG 1 cut(s) 653
Fsp4HI GCNGC 7 cut(s) 247, 671, 722, 730, 733, 828, 1254
FspBI CTAG 5 cut(s) 224, 264, 1224, 1341, 1501
GlaI GCGC 1 cut(s) 1252
GluI GCNGC 7 cut(s) 247, 671, 722, 730, 733, 828, 1254
GsaI CCCAGC 1 cut(s) 1260
GsuI CTGGAG 2 cut(s) 131, 1388
HaeIII GGCC 9 cut(s) 242, 249, 275, 462, 486, 1139, 1185, 1222, 1466
HapII CCGG 4 cut(s) 243, 306, 459, 1319
HgaI GACGC 1 cut(s) 416
HhaI GCGC 1 cut(s) 1253
Hin6I GCGC 1 cut(s) 1251
HinP1I GCGC 1 cut(s) 1251
HindIII AAGCTT 2 cut(s) 1010, 1071
HinfI GANTC 7 cut(s) 11, 309, 692, 860, 875, 965, 1023
HpaII CCGG 4 cut(s) 243, 306, 459, 1319
HphI GGTGA 3 cut(s) 120, 536, 960
Hpy166II GTNNAC 1 cut(s) 1099
Hpy188I TCNGA 9 cut(s) 46, 162, 697, 859, 874, 898, 1087, 1113, 1294
Hpy188III TCNNGA 7 cut(s) 119, 530, 783, 864, 1059, 1319, 1421
Hpy8I GTNNAC 1 cut(s) 1099
HpyAV CCTTC 1 cut(s) 879
HpyCH4III ACNGT 4 cut(s) 218, 392, 467, 947
HpyCH4IV ACGT 3 cut(s) 378, 491, 708
HpyCH4V TGCA 8 cut(s) 577, 605, 670, 836, 1091, 1326, 1355, 1388
HpyF10VI GCNNNNNNNGC 4 cut(s) 833, 1009, 1191, 1259
HpyF3I CTNAG 7 cut(s) 725, 763, 862, 897, 906, 1291, 1443
HpySE526I ACGT 3 cut(s) 378, 491, 708
HspAI GCGC 1 cut(s) 1251
Kpn2I TCCGGA 1 cut(s) 1318
KroI GCCGGC 2 cut(s) 242, 458
KroNI GCCGGC 2 cut(s) 244, 460
Ksp22I TGATCA 3 cut(s) 526, 645, 1204
Kzo9I GATC 7 cut(s) 526, 645, 848, 935, 1108, 1204, 1281
LmnI GCTCC 3 cut(s) 785, 835, 1199
Lsp1109I GCAGC 6 cut(s) 682, 733, 741, 744, 839, 1265
LweI GCATC 3 cut(s) 641, 1078, 1404
MabI ACCWGGT 1 cut(s) 744
MaeI CTAG 5 cut(s) 224, 264, 1224, 1341, 1501
MaeII ACGT 3 cut(s) 378, 491, 708
MaeIII GTNAC 5 cut(s) 218, 287, 400, 473, 1371
MalI GATC 7 cut(s) 528, 647, 850, 937, 1110, 1206, 1283
MbiI CCGCTC 1 cut(s) 1038
MboI GATC 7 cut(s) 526, 645, 848, 935, 1108, 1204, 1281
MboII GAAGA 7 cut(s) 343, 346, 386, 656, 665, 945, 1291
MfeI CAATTG 1 cut(s) 1130
MluCI AATT 9 cut(s) 84, 189, 539, 582, 886, 1130, 1143, 1327, 1473
MlyI GAGTC 2 cut(s) 854, 869
MmeI TCCRAC 1 cut(s) 862
MroI TCCGGA 1 cut(s) 1318
MroNI GCCGGC 2 cut(s) 242, 458
MroXI GAANNNNTTC 1 cut(s) 778
MseI TTAA 4 cut(s) 440, 890, 920, 1014
MslI CAYNNNNRTG 1 cut(s) 216
MspA1I CMGCKG 2 cut(s) 724, 1256
MspI CCGG 4 cut(s) 243, 306, 459, 1319
MspR9I CCNGG 4 cut(s) 306, 746, 1167, 1367
MunI CAATTG 1 cut(s) 1130
Mva1269I GAATGC 2 cut(s) 579, 1357
MvaI CCWGG 3 cut(s) 746, 1167, 1367
MwoI GCNNNNNNNGC 4 cut(s) 833, 1009, 1191, 1259
NaeI GCCGGC 2 cut(s) 244, 460
NciI CCSGG 1 cut(s) 306
NcoI CCATGG 2 cut(s) 75, 1197
NdeII GATC 7 cut(s) 526, 645, 848, 935, 1108, 1204, 1281
NgoMIV GCCGGC 2 cut(s) 242, 458
NlaIV GGNNCC 1 cut(s) 714
NmuCI GTSAC 1 cut(s) 218
NspI RCATGY 1 cut(s) 755
NspV TTCGAA 2 cut(s) 256, 690
OliI CACNNNNGTG 1 cut(s) 216
PagI TCATGA 1 cut(s) 529
PaqCI CACCTGC 1 cut(s) 512
PceI AGGCCT 1 cut(s) 1222
PciI ACATGT 1 cut(s) 751
PctI GAATGC 2 cut(s) 579, 1357
PdiI GCCGGC 2 cut(s) 244, 460
PdmI GAANNNNTTC 1 cut(s) 778
PfeI GAWTC 5 cut(s) 11, 309, 692, 965, 1023
Pfl23II CGTACG 1 cut(s) 488
PkrI GCNGC 7 cut(s) 248, 672, 723, 731, 734, 829, 1255
PleI GAGTC 2 cut(s) 854, 869
PpsI GAGTC 2 cut(s) 854, 869
PpuMI RGGWCCY 1 cut(s) 866
PscI ACATGT 1 cut(s) 751
PsiI TTATAA 1 cut(s) 1242
Psp5II RGGWCCY 1 cut(s) 866
Psp6I CCWGG 3 cut(s) 744, 1165, 1365
PspFI CCCAGC 1 cut(s) 1256
PspGI CCWGG 3 cut(s) 744, 1165, 1365
PspLI CGTACG 1 cut(s) 488
PspN4I GGNNCC 1 cut(s) 714
PspPI GGNCC 5 cut(s) 240, 484, 712, 742, 866
PspPPI RGGWCCY 1 cut(s) 866
PsrI GAACNNNNNNTAC 2 cut(s) 481, 513
PstI CTGCAG 1 cut(s) 672
PstNI CAGNNNCTG 2 cut(s) 788, 833
PvuII CAGCTG 2 cut(s) 724, 1256
RsaI GTAC 6 cut(s) 100, 490, 621, 750, 817, 1496
RsaNI GTAC 6 cut(s) 99, 489, 620, 749, 816, 1495
RseI CAYNNNNRTG 1 cut(s) 216
SaqAI TTAA 4 cut(s) 440, 890, 920, 1014
SatI GCNGC 7 cut(s) 247, 671, 722, 730, 733, 828, 1254
Sau3AI GATC 7 cut(s) 526, 645, 848, 935, 1108, 1204, 1281
Sau96I GGNCC 5 cut(s) 240, 484, 712, 742, 866
ScaI AGTACT 1 cut(s) 621
SchI GAGTC 2 cut(s) 854, 869
ScrFI CCNGG 4 cut(s) 306, 746, 1167, 1367
SexAI ACCWGGT 1 cut(s) 744
SfaNI GCATC 3 cut(s) 641, 1078, 1404
SfcI CTRYAG 1 cut(s) 668
SfuI TTCGAA 2 cut(s) 256, 690
SinI GGWCC 3 cut(s) 712, 742, 866
SmiMI CAYNNNNRTG 1 cut(s) 216
Sse9I AATT 9 cut(s) 84, 189, 539, 582, 886, 1130, 1143, 1327, 1473
SseBI AGGCCT 1 cut(s) 1222
SsiI CCGC 3 cut(s) 166, 246, 1036
SspMI CTAG 5 cut(s) 224, 264, 1224, 1341, 1501
StuI AGGCCT 1 cut(s) 1222
StyD4I CCNGG 4 cut(s) 304, 744, 1165, 1365
StyI CCWWGG 3 cut(s) 75, 1197, 1223
TaaI ACNGT 4 cut(s) 218, 392, 467, 947
TaiI ACGT 3 cut(s) 381, 494, 711
TaqI TCGA 5 cut(s) 36, 256, 690, 916, 1021
TasI AATT 9 cut(s) 84, 189, 539, 582, 886, 1130, 1143, 1327, 1473
TatI WGTACW 2 cut(s) 98, 619
TauI GCSGC 1 cut(s) 249
TfiI GAWTC 5 cut(s) 11, 309, 692, 965, 1023
Tru1I TTAA 4 cut(s) 440, 890, 920, 1014
Tru9I TTAA 4 cut(s) 440, 890, 920, 1014
TscAI CASTG 2 cut(s) 223, 1319
TseFI GTSAC 1 cut(s) 218
TseI GCWGC 6 cut(s) 670, 721, 729, 732, 827, 1253
Tsp45I GTSAC 1 cut(s) 218
TspDTI ATGAA 4 cut(s) 142, 387, 600, 1364
TspGWI ACGGA 1 cut(s) 594
TspRI CASTG 2 cut(s) 223, 1319
VpaK11BI GGWCC 3 cut(s) 712, 742, 866
XapI RAATTY 3 cut(s) 582, 886, 1143
XceI RCATGY 1 cut(s) 755
XmaJI CCTAGG 1 cut(s) 1223
XmiI GTMKAC 1 cut(s) 1098
XmnI GAANNNNTTC 1 cut(s) 778
XspI CTAG 5 cut(s) 224, 264, 1224, 1341, 1501
ZrmI AGTACT 1 cut(s) 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.