pycom06g11100
BHLH Family

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0000212
Physical Location & Seq
Forward (+)
16337649 .. 16339516
1868 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g11100.1

Sequence Viewer

Length: 1551 bp
ATGGAAGAAATCATGTCCTCATGTACTCCACCCAATTTCTGCCGAGAAACCTCCTCCAAATTTCAACAGAGCCTACAGTTCATAATTCAGAACAGGCCTGAGTGGTGGGTGTATTCCATTTTCTGGCAAGCCTCCAAAGACAGCATTAGTGACCAAGTTTCCCTGTCGTGGGCTGCCGGCCATTTCCAGGACACTCGCGATCACTTGGAATCGAAAAGAAGCAACCGAGTGACCAACAGTTACCAACCCAAATTTGGGTTCAATAATGTGGGGAGAAAGAAGACGATTAACCGGGAAGTTGAAGCTCTTTTGCATGATGACATGGTGGATTTGGACATGAGGTTGGTGGATCAGGGAGATGTGGCTGACACTGAGTGGTTTTACTCTTACACCGTTTCTTTAACCCAGTCATTCTCTGCAAGCCACGGTAATAATAGTGACAATATTCTCGGCAGGGCATTTGGTTCTGGTGGGTTTGTTTGGCTTGCAGCTGACCATGAATTTCAATTTTATGACTGTGAGAGAGTGAAAGAAGCAAGAATGAATGGGATACAAACTTTGGTTTGCATTGCAACTCCTTGTGGGGTACTTGAACTGGCCTCTTTGGATGTGATCAAAGAAGATTGGGGTCTAGTGCAGTTATCAAAGTCGCTGTTCGGATCAGACGGCAACATCAACAAGACGCTCTCAAAGCAGACCAGCCATGACGGCAATGTAATTGCTCCTATCCTAGAAAATGGACTGTTTTCAGCAGCTGAAAAGGAACTCACCCGACAAGATCGAGGTGGTATAAACGAAGCTGCACCTATCAATCTAGGACGGTCGTCACCGGAATCACCTTCTGACTCCGTTGACAATTTCACCTCCGAGAATACGGAGAGCATGACTCGGACAAAAAAGCGAGGGAGATCATCAAACCATGGATCTGCAAGTAGAGAATCACAATTGTTGAACCACGTTGAGGCAGAGAGACAGAGGAGGGAGAAGCTCAACCACCGATTCTATGTCCTCCGGTCAGTTGTTCCCAATGTGTCCAAAATGGACAGGTCCTCTTTGCTTGCCGATGCCGTGGCGTACATCAATCAACTCAAATCAAAAGTTGAGGAATTGGAGGCCAAAGTCCAAGAACAACCACAAAAACCTAAAGCGTGCAATAATGCAAGTAACAGTCTTGATCACCACTCTGGCCAAAGCACCAGCTCCAAAGTCGAACGGCGTCATTGTTCTAATAATAATGATAGTAATATTATTAGTAGTAATAACAACAATAATAATAATAATATTAATAGTGATAGGGGAGCTGCTGCGGATGTGGAAGTGGATGTAAAGATTATGGGGTCAGAAGCCATAATACATGTTCAGTGTCCGGATCAAGGCTACCCATATGCTAAGTTGATGAAGGCACTCAAAGGCCTTGGGCTACAAGTTTACCATGCAAGCATCTCGAGCGTGAAAGAGTTGATGATTCAGGATGTTGTGGCAAGACTGCCTTATGGGTTCAATGGTGAGGAGGCATTGAGAAATGGTATTATAAAAAGATGGTACCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 map04016 map04075
Pfam Domains
Protein Families
HLH bHLH-MYC_N

Protein Analysis

517

Amino Acids

57.81

Weight (kDa)

6.3

Isoelectric Point (pI)

43.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH-MYC_N PF14215 22 - 219 4.5e-40 bHLH-MYC and R2R3-MYB transcription factors N-terminal
HLH PF00010 318 - 364 2.1e-10 Helix-loop-helix DNA-binding domain
bHLH-TF_ACT-like_plant PF22754 439 - 495 1.6e-07 Plant bHLH transcription factor, ACT-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000212)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G00870
fragaria_vesca FvH4_5g10850 FvH4_5g10851 FvH4_5g23290 FvH4_7g17380
malus_domestica MD00G1137900.v1.1 MD01G1086800.v1.1 MD01G1086900.v1.1 MD03G1096500.v1.1 MD06G1119900.v1.1 MD06G1120000.v1.1 MD06G1120100.v1.1 MD06G1120200.v1.1 MD11G1120400.v1.1 MD11G1145000.v1.1 MD11G1254000.v1.1 MD11G1254100.v1.1 MD14G1126900.v1.1 MD14G1127000.v1.1 MD14G1137200.v1.1
prunus_persica Prupe.2G195300_v2.0.a1 Prupe.2G195300_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087900_v2.0.a1 Prupe.5G130300_v2.0.a1 Prupe.5G130400_v2.0.a1 Prupe.5G130500_v2.0.a1 Prupe.5G130500_v2.0.a1 Prupe.5G130600_v2.0.a1 Prupe.5G130700_v2.0.a1
pyrus_communis pycom01g09980 pycom06g11100 pycom06g11120 pycom06g11150 pycom11g10170 pycom11g22490 pycom14g11190 pycom14g11210
rosa_chinensis RchiOBHm_Chr1g0360811 RchiOBHm_Chr5g0055891 RchiOBHm_Chr6g0281801 RchiOBHm_Chr7g0187141 RchiOBHm_Chr7g0187261 RchiOBHm_Chr7g0212241 RchiOBHm_Chr7g0228701
rosa_laevigata RLG00000002910 RLG00000002911 RLG00000004796 RLG00000004799 RLG00000027784
rosa_multiflora Rmu_sc0000340.1_g000036 Rmu_sc0000378.1_g000013 Rmu_sc0000441.1_g000071 Rmu_sc0000559.1_g000036 Rmu_sc0000637.1_g000017 Rmu_sc0000765.1_g000001 Rmu_sc0000785.1_g000012 Rmu_sc0000854.1_g000018 Rmu_sc0000959.1_g000018 Rmu_sc0001018.1_g000022 Rmu_sc0001136.1_g000078 Rmu_sc0001433.1_g000037 Rmu_sc0001687.1_g000010 Rmu_sc0001716.1_g000014 Rmu_sc0001758.1_g000023 Rmu_sc0001758.1_g000031 Rmu_sc0001826.1_g000009 Rmu_sc0001850.1_g000027 Rmu_sc0001911.1_g000019 Rmu_sc0001937.1_g000001 Rmu_sc0002283.1_g000088 Rmu_sc0002532.1_g000058 Rmu_sc0002571.1_g000032 Rmu_sc0002888.1_g000014 Rmu_sc0002939.1_g000019 Rmu_sc0003197.1_g000007 Rmu_sc0003546.1_g000034 Rmu_sc0004002.1_g000003 Rmu_sc0004145.1_g000003 Rmu_sc0004301.1_g000013 Rmu_sc0004603.1_g000002 Rmu_sc0004748.1_g000007 Rmu_sc0004842.1_g000002 Rmu_sc0004990.1_g000012 Rmu_sc0005017.1_g000006 Rmu_sc0006343.1_g000001 Rmu_sc0006836.1_g000017 Rmu_sc0007491.1_g000003 Rmu_sc0008192.1_g000011 Rmu_sc0008877.1_g000007 Rmu_sc0010621.1_g000006 Rmu_sc0013354.1_g000017 Rmu_sc0013486.1_g000001 Rmu_sc0016511.1_g000001 Rmu_sc0021179.1_g000001 Rmu_sc0026563.1_g000001 Rmu_sc0027421.1_g000001 Rmu_sc0037114.1_g000001 Rmu_ssc0000022.1_g000039 Rmu_ssc0000050.1_g000048 Rmu_ssc0000141.1_g000004 Rmu_ssc0000280.1_g000002 Rmu_ssc0000387.1_g000024 Rmu_ssc0000459.1_g000030
rosa_roxburghii Rroxscaffold_3G00247010 Rroxscaffold_3G00267630 Rroxscaffold_3G00267670 Rroxscaffold_4G00295790 Rroxscaffold_4G00305930 Rroxscaffold_5G00346440 Rroxscaffold_7G00211970
rosa_rugosa Rorug01G0281800 Rorug02G0258400 Rorug04G0057400 Rorug04G0362000 Rorug04G0437300 Rorug06G0161400 Rorug06G0213300 Rorug06G0478800 Rorug06G0479300 Rorug06G0479300 Rorug07G0132200 Rorug07G0321200
rosa_samantha Rh1AG293700 Rh1BG258400 Rh1CG276200 Rh1DG288700 Rh7AG084400 Rh7AG084900 Rh7AG265100 Rh7BG078100 Rh7BG078600 Rh7CG085500 Rh7CG085800 Rh7CG281900 Rh7DG086500 Rh7DG086900 Rh7DG272300
rosa_wichuraiana Rw1G025990 Rw7G007440 Rw7G007460 Rw7G022700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1532
Acc65I GGTACC 1 cut(s) 1542
AccB1I GGYRCC 1 cut(s) 1542
AccB7I CCANNNNNTGG 1 cut(s) 123
AccII CGCG 1 cut(s) 198
AccIII TCCGGA 1 cut(s) 1366
AciI CCGC 1 cut(s) 1307
AclWI GGATC 4 cut(s) 357, 667, 931, 1377
AcoI YGGCCR 2 cut(s) 178, 1186
AcsI RAATTY 3 cut(s) 59, 251, 500
AcyI GRCGYC 1 cut(s) 1216
AdeI CACNNNGTG 1 cut(s) 375
AfaI GTAC 4 cut(s) 25, 588, 1076, 1544
AfiI CCNNNNNNNGG 8 cut(s) 123, 168, 169, 187, 254, 255, 961, 1373
AflIII ACRYGT 1 cut(s) 1354
AgsI TTSAA 7 cut(s) 65, 262, 302, 506, 593, 952, 1501
AjnI CCWGG 1 cut(s) 186
AjuI GAANNNNNNNTTGG 2 cut(s) 242, 274
AluBI AGCT 7 cut(s) 305, 491, 755, 800, 988, 1200, 1301
AluI AGCT 7 cut(s) 305, 491, 755, 800, 988, 1200, 1301
Alw26I GTCTC 1 cut(s) 964
AlwI GGATC 4 cut(s) 357, 667, 931, 1377
AlwNI CAGNNNCTG 1 cut(s) 755
Ama87I CYCGRG 1 cut(s) 1444
Aor13HI TCCGGA 1 cut(s) 1366
AoxI GGCC 6 cut(s) 95, 178, 597, 1113, 1186, 1411
ApeKI GCWGC 6 cut(s) 173, 488, 752, 800, 1301, 1304
ApoI RAATTY 3 cut(s) 59, 251, 500
AseI ATTAAT 1 cut(s) 1284
Asp718I GGTACC 1 cut(s) 1542
AspS9I GGNCC 1 cut(s) 1047
AsuC2I CCSGG 1 cut(s) 293
AsuHPI GGTGA 6 cut(s) 760, 819, 828, 853, 1169, 1517
AvaI CYCGRG 1 cut(s) 1444
AvaII GGWCC 1 cut(s) 1047
BalI TGGCCA 1 cut(s) 1188
BanI GGYRCC 1 cut(s) 1542
BarI GAAGNNNNNNTAC 2 cut(s) 1335, 1367
BbsI GAAGAC 1 cut(s) 287
BbvI GCAGC 6 cut(s) 160, 500, 764, 787, 1288, 1291
BccI CCATC 1 cut(s) 1533
BceAI ACGGC 4 cut(s) 682, 724, 1052, 1229
BcgI CGANNNNNNTGC 2 cut(s) 1425, 1459
BciT130I CCWGG 1 cut(s) 188
BciVI GTATCC 1 cut(s) 543
BclI TGATCA 2 cut(s) 612, 1174
BcnI CCSGG 1 cut(s) 293
BcoDI GTCTC 1 cut(s) 964
BfaI CTAG 3 cut(s) 632, 731, 815
BfmI CTRYAG 1 cut(s) 74
BfuI GTATCC 1 cut(s) 543
BglI GCCNNNNNGGC 1 cut(s) 708
BisI GCNGC 6 cut(s) 174, 489, 753, 801, 1302, 1305
BlsI GCNGC 6 cut(s) 175, 490, 754, 802, 1303, 1306
Bme1390I CCNGG 2 cut(s) 188, 293
Bme18I GGWCC 1 cut(s) 1047
BmeT110I CYCGRG 1 cut(s) 1444
BmgT120I GGNCC 1 cut(s) 1047
BmiI GGNNCC 1 cut(s) 1544
BmrFI CCNGG 2 cut(s) 188, 293
BmrI ACTGGG 1 cut(s) 400
BmsI GCATC 2 cut(s) 1054, 1449
BmuI ACTGGG 1 cut(s) 400
BoxI GACNNNNGTC 1 cut(s) 823
BpiI GAAGAC 1 cut(s) 287
BplI GAGNNNNNCTC 2 cut(s) 871, 903
BpuMI CCSGG 1 cut(s) 293
BsaHI GRCGYC 1 cut(s) 1216
BsaJI CCNNGG 4 cut(s) 424, 919, 1068, 1414
BsaWI WCCGGW 3 cut(s) 829, 1011, 1366
Bsc4I CCNNNNNNNGG 8 cut(s) 123, 168, 169, 187, 254, 255, 961, 1373
Bse118I RCCGGY 1 cut(s) 176
Bse1I ACTGG 2 cut(s) 406, 600
Bse3DI GCAATG 2 cut(s) 567, 718
BseAI TCCGGA 1 cut(s) 1366
BseBI CCWGG 1 cut(s) 188
BseDI CCNNGG 4 cut(s) 424, 919, 1068, 1414
BseGI GGATG 4 cut(s) 613, 1315, 1327, 1477
BseLI CCNNNNNNNGG 8 cut(s) 123, 168, 169, 187, 254, 255, 961, 1373
BseMI GCAATG 2 cut(s) 567, 718
BseMII CTCAG 2 cut(s) 90, 363
BseNI ACTGG 2 cut(s) 406, 600
BseRI GAGGAG 3 cut(s) 43, 991, 1523
BseXI GCAGC 6 cut(s) 160, 500, 764, 787, 1288, 1291
BsgI GTGCAG 2 cut(s) 656, 786
Bsh1236I CGCG 1 cut(s) 198
Bsh1285I CGRYCG 1 cut(s) 824
BshFI GGCC 6 cut(s) 97, 180, 599, 1115, 1188, 1413
BshNI GGYRCC 1 cut(s) 1542
BsiEI CGRYCG 1 cut(s) 824
BsiHKCI CYCGRG 1 cut(s) 1444
BsiSI CCGG 5 cut(s) 177, 292, 830, 1012, 1367
BslI CCNNNNNNNGG 8 cut(s) 123, 168, 169, 187, 254, 255, 961, 1373
BsmAI GTCTC 1 cut(s) 964
BsnI GGCC 6 cut(s) 97, 180, 599, 1115, 1188, 1413
BsoBI CYCGRG 1 cut(s) 1444
Bsp13I TCCGGA 1 cut(s) 1366
Bsp143I GATC 9 cut(s) 199, 349, 612, 659, 778, 908, 923, 1174, 1369
Bsp19I CCATGG 1 cut(s) 919
Bsp68I TCGCGA 1 cut(s) 198
BspACI CCGC 1 cut(s) 1307
BspANI GGCC 6 cut(s) 97, 180, 599, 1115, 1188, 1413
BspCNI CTCAG 2 cut(s) 91, 364
BspEI TCCGGA 1 cut(s) 1366
BspFNI CGCG 1 cut(s) 198
BspLI GGNNCC 1 cut(s) 1544
BspPI GGATC 4 cut(s) 357, 667, 931, 1377
BspT107I GGYRCC 1 cut(s) 1542
BsrDI GCAATG 2 cut(s) 567, 718
BsrFI RCCGGY 1 cut(s) 176
BsrI ACTGG 2 cut(s) 406, 600
BssAI RCCGGY 1 cut(s) 176
BssECI CCNNGG 4 cut(s) 424, 919, 1068, 1414
BssMI GATC 9 cut(s) 199, 349, 612, 659, 778, 908, 923, 1174, 1369
BssNI GRCGYC 1 cut(s) 1216
BssT1I CCWWGG 2 cut(s) 919, 1414
Bst2UI CCWGG 1 cut(s) 188
Bst4CI ACNGT 9 cut(s) 78, 239, 394, 428, 518, 744, 822, 1169, 1547
BstACI GRCGYC 1 cut(s) 1216
BstC8I GCNNGC 7 cut(s) 129, 178, 421, 486, 1059, 1150, 1438
BstDEI CTNAG 3 cut(s) 99, 372, 1389
BstDSI CCRYGG 3 cut(s) 424, 919, 1068
BstF5I GGATG 4 cut(s) 613, 1315, 1327, 1477
BstFNI CGCG 1 cut(s) 198
BstKTI GATC 9 cut(s) 202, 352, 615, 662, 781, 911, 926, 1177, 1372
BstMAI GTCTC 1 cut(s) 964
BstMBI GATC 9 cut(s) 199, 349, 612, 659, 778, 908, 923, 1174, 1369
BstMCI CGRYCG 1 cut(s) 824
BstMWI GCNNNNNNNGC 3 cut(s) 691, 708, 1446
BstNI CCWGG 1 cut(s) 188
BstNSI RCATGY 1 cut(s) 1358
BstPAI GACNNNNGTC 1 cut(s) 823
BstSCI CCNGG 2 cut(s) 186, 291
BstSFI CTRYAG 1 cut(s) 74
BstUI CGCG 1 cut(s) 198
BstV1I GCAGC 6 cut(s) 160, 500, 764, 787, 1288, 1291
BstV2I GAAGAC 1 cut(s) 287
BstX2I RGATCY 1 cut(s) 923
BstYI RGATCY 1 cut(s) 923
BsuI GTATCC 1 cut(s) 543
BsuRI GGCC 6 cut(s) 97, 180, 599, 1115, 1188, 1413
BtgI CCRYGG 3 cut(s) 424, 919, 1068
BtsCI GGATG 4 cut(s) 613, 1315, 1327, 1477
BtsIMutI CAGTG 2 cut(s) 369, 1367
BtuMI TCGCGA 1 cut(s) 198
Cac8I GCNNGC 7 cut(s) 129, 178, 421, 486, 1059, 1150, 1438
CaiI CAGNNNCTG 1 cut(s) 755
Cfr10I RCCGGY 1 cut(s) 176
Cfr13I GGNCC 1 cut(s) 1047
CseI GACGC 2 cut(s) 691, 1205
Csp6I GTAC 4 cut(s) 24, 587, 1075, 1543
CviQI GTAC 4 cut(s) 24, 587, 1075, 1543
DdeI CTNAG 3 cut(s) 99, 372, 1389
DpnI GATC 9 cut(s) 201, 351, 614, 661, 780, 910, 925, 1176, 1371
DpnII GATC 9 cut(s) 199, 349, 612, 659, 778, 908, 923, 1174, 1369
DraIII CACNNNGTG 1 cut(s) 375
EaeI YGGCCR 2 cut(s) 178, 1186
Eco130I CCWWGG 2 cut(s) 919, 1414
Eco147I AGGCCT 2 cut(s) 97, 1413
Eco47I GGWCC 1 cut(s) 1047
Eco88I CYCGRG 1 cut(s) 1444
EcoO109I RGGNCCY 1 cut(s) 1047
EcoRII CCWGG 1 cut(s) 186
EcoT14I CCWWGG 2 cut(s) 919, 1414
ErhI CCWWGG 2 cut(s) 919, 1414
FalI AAGNNNNNCTT 2 cut(s) 1474, 1506
FauNDI CATATG 1 cut(s) 1384
FbaI TGATCA 2 cut(s) 612, 1174
Fnu4HI GCNGC 6 cut(s) 174, 489, 753, 801, 1302, 1305
FokI GGATG 4 cut(s) 620, 1322, 1334, 1484
Fsp4HI GCNGC 6 cut(s) 174, 489, 753, 801, 1302, 1305
FspBI CTAG 3 cut(s) 632, 731, 815
GluI GCNGC 6 cut(s) 174, 489, 753, 801, 1302, 1305
HaeIII GGCC 6 cut(s) 97, 180, 599, 1115, 1188, 1413
HapII CCGG 5 cut(s) 177, 292, 830, 1012, 1367
HgaI GACGC 2 cut(s) 691, 1205
Hin1I GRCGYC 1 cut(s) 1216
HincII GTYRAC 1 cut(s) 853
HindII GTYRAC 1 cut(s) 853
HinfI GANTC 7 cut(s) 209, 833, 845, 886, 938, 999, 1465
HpaII CCGG 5 cut(s) 177, 292, 830, 1012, 1367
HphI GGTGA 6 cut(s) 760, 819, 828, 853, 1169, 1517
Hpy166II GTNNAC 2 cut(s) 853, 1429
Hpy188I TCNGA 7 cut(s) 90, 659, 664, 844, 868, 891, 1342
Hpy188III TCNNGA 5 cut(s) 197, 1172, 1367, 1444, 1469
Hpy8I GTNNAC 2 cut(s) 853, 1429
HpyAV CCTTC 2 cut(s) 849, 1393
HpyCH4III ACNGT 9 cut(s) 78, 239, 394, 428, 518, 744, 822, 1169, 1547
HpyCH4IV ACGT 1 cut(s) 957
HpyF10VI GCNNNNNNNGC 3 cut(s) 691, 708, 1446
HpyF3I CTNAG 3 cut(s) 99, 372, 1389
HpySE526I ACGT 1 cut(s) 957
Hsp92I GRCGYC 1 cut(s) 1216
Kpn2I TCCGGA 1 cut(s) 1366
KpnI GGTACC 1 cut(s) 1546
KroI GCCGGC 1 cut(s) 176
KroNI GCCGGC 1 cut(s) 178
Ksp22I TGATCA 2 cut(s) 612, 1174
Kzo9I GATC 9 cut(s) 199, 349, 612, 659, 778, 908, 923, 1174, 1369
LmnI GCTCC 3 cut(s) 727, 1205, 1298
Lsp1109I GCAGC 6 cut(s) 160, 500, 764, 787, 1288, 1291
LweI GCATC 2 cut(s) 1054, 1449
MaeI CTAG 3 cut(s) 632, 731, 815
MaeII ACGT 1 cut(s) 957
MaeIII GTNAC 6 cut(s) 149, 229, 239, 437, 825, 1163
MalI GATC 9 cut(s) 201, 351, 614, 661, 780, 910, 925, 1176, 1371
MboI GATC 9 cut(s) 199, 349, 612, 659, 778, 908, 923, 1174, 1369
MboII GAAGA 3 cut(s) 17, 292, 632
MfeI CAATTG 1 cut(s) 944
MflI RGATCY 1 cut(s) 923
MlsI TGGCCA 1 cut(s) 1188
MluNI TGGCCA 1 cut(s) 1188
MlyI GAGTC 2 cut(s) 839, 880
Mox20I TGGCCA 1 cut(s) 1188
MroI TCCGGA 1 cut(s) 1366
MroNI GCCGGC 1 cut(s) 176
MscI TGGCCA 1 cut(s) 1188
MseI TTAA 3 cut(s) 288, 401, 1284
Msp20I TGGCCA 1 cut(s) 1188
MspA1I CMGCKG 2 cut(s) 491, 755
MspI CCGG 5 cut(s) 177, 292, 830, 1012, 1367
MspR9I CCNGG 2 cut(s) 188, 293
MunI CAATTG 1 cut(s) 944
MvaI CCWGG 1 cut(s) 188
MvnI CGCG 1 cut(s) 198
MwoI GCNNNNNNNGC 3 cut(s) 691, 708, 1446
NaeI GCCGGC 1 cut(s) 178
NciI CCSGG 1 cut(s) 293
NcoI CCATGG 1 cut(s) 919
NdeI CATATG 1 cut(s) 1384
NdeII GATC 9 cut(s) 199, 349, 612, 659, 778, 908, 923, 1174, 1369
NgoMIV GCCGGC 1 cut(s) 176
NlaIV GGNNCC 1 cut(s) 1544
NmeAIII GCCGAG 2 cut(s) 68, 429
NmuCI GTSAC 4 cut(s) 149, 229, 437, 825
NruI TCGCGA 1 cut(s) 198
NspI RCATGY 1 cut(s) 1358
PaeR7I CTCGAG 1 cut(s) 1444
PceI AGGCCT 2 cut(s) 97, 1413
PciI ACATGT 1 cut(s) 1354
PdiI GCCGGC 1 cut(s) 178
PfeI GAWTC 5 cut(s) 209, 833, 938, 999, 1465
PflMI CCANNNNNTGG 1 cut(s) 123
PfoI TCCNGGA 1 cut(s) 186
PkrI GCNGC 6 cut(s) 175, 490, 754, 802, 1303, 1306
PleI GAGTC 2 cut(s) 839, 880
PpsI GAGTC 2 cut(s) 839, 880
PpuMI RGGWCCY 1 cut(s) 1047
PscI ACATGT 1 cut(s) 1354
PshAI GACNNNNGTC 1 cut(s) 823
PshBI ATTAAT 1 cut(s) 1284
PsiI TTATAA 1 cut(s) 1532
Psp5II RGGWCCY 1 cut(s) 1047
Psp6I CCWGG 1 cut(s) 186
PspGI CCWGG 1 cut(s) 186
PspN4I GGNNCC 1 cut(s) 1544
PspPI GGNCC 1 cut(s) 1047
PspPPI RGGWCCY 1 cut(s) 1047
PstNI CAGNNNCTG 1 cut(s) 755
PsuI RGATCY 1 cut(s) 923
PvuII CAGCTG 2 cut(s) 491, 755
RruI TCGCGA 1 cut(s) 198
RsaI GTAC 4 cut(s) 25, 588, 1076, 1544
RsaNI GTAC 4 cut(s) 24, 587, 1075, 1543
SaqAI TTAA 3 cut(s) 288, 401, 1284
SatI GCNGC 6 cut(s) 174, 489, 753, 801, 1302, 1305
Sau3AI GATC 9 cut(s) 199, 349, 612, 659, 778, 908, 923, 1174, 1369
Sau96I GGNCC 1 cut(s) 1047
SchI GAGTC 2 cut(s) 839, 880
ScrFI CCNGG 2 cut(s) 188, 293
SfaNI GCATC 2 cut(s) 1054, 1449
SfcI CTRYAG 1 cut(s) 74
Sfr274I CTCGAG 1 cut(s) 1444
SinI GGWCC 1 cut(s) 1047
SlaI CTCGAG 1 cut(s) 1444
SmlI CTYRAG 1 cut(s) 1444
SmoI CTYRAG 1 cut(s) 1444
SseBI AGGCCT 2 cut(s) 97, 1413
SsiI CCGC 1 cut(s) 1307
SspI AATATT 3 cut(s) 445, 1246, 1282
SspMI CTAG 3 cut(s) 632, 731, 815
StuI AGGCCT 2 cut(s) 97, 1413
StyD4I CCNGG 2 cut(s) 186, 291
StyI CCWWGG 2 cut(s) 919, 1414
TaaI ACNGT 9 cut(s) 78, 239, 394, 428, 518, 744, 822, 1169, 1547
TaiI ACGT 1 cut(s) 960
TaqI TCGA 4 cut(s) 212, 781, 1209, 1445
TatI WGTACW 1 cut(s) 23
TfiI GAWTC 5 cut(s) 209, 833, 938, 999, 1465
Tru1I TTAA 3 cut(s) 288, 401, 1284
Tru9I TTAA 3 cut(s) 288, 401, 1284
TscAI CASTG 2 cut(s) 376, 1367
TseFI GTSAC 4 cut(s) 149, 229, 437, 825
TseI GCWGC 6 cut(s) 173, 488, 752, 800, 1301, 1304
Tsp45I GTSAC 4 cut(s) 149, 229, 437, 825
TspDTI ATGAA 4 cut(s) 70, 513, 557, 1412
TspGWI ACGGA 2 cut(s) 838, 890
TspRI CASTG 2 cut(s) 376, 1367
Van91I CCANNNNNTGG 1 cut(s) 123
VpaK11BI GGWCC 1 cut(s) 1047
VspI ATTAAT 1 cut(s) 1284
XapI RAATTY 3 cut(s) 59, 251, 500
XceI RCATGY 1 cut(s) 1358
XcmI CCANNNNNNNNNTGG 1 cut(s) 251
XhoI CTCGAG 1 cut(s) 1444
XspI CTAG 3 cut(s) 632, 731, 815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.