MD14G1127000.v1.1
BHLH Family

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0000212
Physical Location & Seq
Reverse (-)
20216244 .. 20218150
1907 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1127000.v1.1.491

Sequence Viewer

Length: 1554 bp
ATGGAAGAAATCATATCCTCATGTTCTCCACCCACTTTCTGCCAAGAAACCTCCTCCACATTTCAACAGCGCCTACAGTTCATAGTTCAGAACAGGCCCGAGTGGTGGGTGTATTCCATTTTCTGGCAAGCCTCCAAAGTCAGCATTAGTGACCAAGTTTCCCTGTCATGGGCCGGCGGCCATTTTTGGAACACTCACGATCACTTGGCATCGAAAAGAAGCAGTGGAGTGATCAACAGTTACCAACCCAAGTTTGGGTTCAGTAATGGAGAGAGAAGTAAGGTGATTAACCAGGAAGTTGAAGCTCTTTTGCATGATGACATGGTGGATTTGGACATGAGGCTCGTGGATCATGAAGATGCCACTGACTCCGAGTGGTTTTACTTTTATACCGTTTCTTTAACCCAGTCATTCTCTGCAGGCCAGGGTAATAATAGTAAAAATATTGTCGGCAGGGCATTTGGTTCTGGTGGGTTTGTTTGGCTTGCAGGTGACCACGACTTTCAGTTTTATGAGTGTGAGAGAGTCAAAGAAGCAAGAATGCATGGAATCCAAACTTTGGTTTGCATTGCAACTCCTTGTGGGGTACTTGAACTGGCCTCTTTGGATGTTATCAAAGAAGACTGGGGTCTTGTGCATTTATCAAAGTCGCTGTTCGGATCAGACGGCAACATCAACATGAACAACAAGAAGAAGCTCTCAAAGCAGCAGACCACCCGGGATGGCAATGTACTTGTTCCTATCCTAGAAAATGGACTTTTTTCAGCAGCTCAAAAGGAATTGATTCGGCAAGATCATGGTGGTAGTATAAACGAAGCTGCACCTATCAATCTAGGAGAATCGTCACCGGAAACACCTTCTGACTCCGTTGGCAACTTCACATTGGAGAATACGGAGAACATGAATCGGTTGAGAAAGAGAGGAAGATCATCAAACCATGGAGCTGCAAGTAGAGAATTACAATTGTTAAACCACGTTGAGGCCGAGAGACAGAGGAGGGAGAAGCTCAACCACCGATTCTATGTCCTCCGGTCAGTTGTTCCCAATGTGTCAAAAATGGACAGGTCCTCTTTGCTTGCCGATGCCGTGTTGTACATCAATCAACTCAAATCAAAAGTTGAGGAATTGGAGGCAAAAGTCCAAGAACAAACACAAAAACCTAAAGCGGGCAATAATGCAAGTAACAATCTTGATCATCACTCCAGCCAAAGCACCAGCTCCATCGTTGACCAACATCATTATTCGTATTATAATATTAGTAATAATAGCAATAGTAATAATAACAGTATTAGTGATAGGGCAGCAGCTGCGGCTGTGGAAGTGGATGTGAAGATTTTTGGGTCAGACGTCATTATACGTGTTCAGTGTCCGGATCAAGACTACCCATATGCTAAGTTGATGAATGCACTCAAGGGCCTAGGGCTACAAGTTTACCATGCAAGCATATGGAGCGTGAAAGAGTTGATGGTTCAAGATGTTGTGGCAAGATTGCCTTATGGGTTTACTGGTAAGGAGGCATTGAGAAATGGTATTATGAAAGGATGGTACTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 map04016 map04075
Pfam Domains
Protein Families
HLH bHLH-MYC_N

Protein Analysis

518

Amino Acids

57.99

Weight (kDa)

6.2

Isoelectric Point (pI)

39.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH-MYC_N PF14215 22 - 219 1.5e-40 bHLH-MYC and R2R3-MYB transcription factors N-terminal
HLH PF00010 324 - 370 1e-09 Helix-loop-helix DNA-binding domain
bHLH-TF_ACT-like_plant PF22754 440 - 494 3.9e-06 Plant bHLH transcription factor, ACT-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000212)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G00870
fragaria_vesca FvH4_5g10850 FvH4_5g10851 FvH4_5g23290 FvH4_7g17380
malus_domestica MD00G1137900.v1.1 MD01G1086800.v1.1 MD01G1086900.v1.1 MD03G1096500.v1.1 MD06G1119900.v1.1 MD06G1120000.v1.1 MD06G1120100.v1.1 MD06G1120200.v1.1 MD11G1120400.v1.1 MD11G1145000.v1.1 MD11G1254000.v1.1 MD11G1254100.v1.1 MD14G1126900.v1.1 MD14G1127000.v1.1 MD14G1137200.v1.1
prunus_persica Prupe.2G195300_v2.0.a1 Prupe.2G195300_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087900_v2.0.a1 Prupe.5G130300_v2.0.a1 Prupe.5G130400_v2.0.a1 Prupe.5G130500_v2.0.a1 Prupe.5G130500_v2.0.a1 Prupe.5G130600_v2.0.a1 Prupe.5G130700_v2.0.a1
pyrus_communis pycom01g09980 pycom06g11100 pycom06g11120 pycom06g11150 pycom11g10170 pycom11g22490 pycom14g11190 pycom14g11210
rosa_chinensis RchiOBHm_Chr1g0360811 RchiOBHm_Chr5g0055891 RchiOBHm_Chr6g0281801 RchiOBHm_Chr7g0187141 RchiOBHm_Chr7g0187261 RchiOBHm_Chr7g0212241 RchiOBHm_Chr7g0228701
rosa_laevigata RLG00000002910 RLG00000002911 RLG00000004796 RLG00000004799 RLG00000027784
rosa_multiflora Rmu_sc0000340.1_g000036 Rmu_sc0000378.1_g000013 Rmu_sc0000441.1_g000071 Rmu_sc0000559.1_g000036 Rmu_sc0000637.1_g000017 Rmu_sc0000765.1_g000001 Rmu_sc0000785.1_g000012 Rmu_sc0000854.1_g000018 Rmu_sc0000959.1_g000018 Rmu_sc0001018.1_g000022 Rmu_sc0001136.1_g000078 Rmu_sc0001433.1_g000037 Rmu_sc0001687.1_g000010 Rmu_sc0001716.1_g000014 Rmu_sc0001758.1_g000023 Rmu_sc0001758.1_g000031 Rmu_sc0001826.1_g000009 Rmu_sc0001850.1_g000027 Rmu_sc0001911.1_g000019 Rmu_sc0001937.1_g000001 Rmu_sc0002283.1_g000088 Rmu_sc0002532.1_g000058 Rmu_sc0002571.1_g000032 Rmu_sc0002888.1_g000014 Rmu_sc0002939.1_g000019 Rmu_sc0003197.1_g000007 Rmu_sc0003546.1_g000034 Rmu_sc0004002.1_g000003 Rmu_sc0004145.1_g000003 Rmu_sc0004301.1_g000013 Rmu_sc0004603.1_g000002 Rmu_sc0004748.1_g000007 Rmu_sc0004842.1_g000002 Rmu_sc0004990.1_g000012 Rmu_sc0005017.1_g000006 Rmu_sc0006343.1_g000001 Rmu_sc0006836.1_g000017 Rmu_sc0007491.1_g000003 Rmu_sc0008192.1_g000011 Rmu_sc0008877.1_g000007 Rmu_sc0010621.1_g000006 Rmu_sc0013354.1_g000017 Rmu_sc0013486.1_g000001 Rmu_sc0016511.1_g000001 Rmu_sc0021179.1_g000001 Rmu_sc0026563.1_g000001 Rmu_sc0027421.1_g000001 Rmu_sc0037114.1_g000001 Rmu_ssc0000022.1_g000039 Rmu_ssc0000050.1_g000048 Rmu_ssc0000141.1_g000004 Rmu_ssc0000280.1_g000002 Rmu_ssc0000387.1_g000024 Rmu_ssc0000459.1_g000030
rosa_roxburghii Rroxscaffold_3G00247010 Rroxscaffold_3G00267630 Rroxscaffold_3G00267670 Rroxscaffold_4G00295790 Rroxscaffold_4G00305930 Rroxscaffold_5G00346440 Rroxscaffold_7G00211970
rosa_rugosa Rorug01G0281800 Rorug02G0258400 Rorug04G0057400 Rorug04G0362000 Rorug04G0437300 Rorug06G0161400 Rorug06G0213300 Rorug06G0478800 Rorug06G0479300 Rorug06G0479300 Rorug07G0132200 Rorug07G0321200
rosa_samantha Rh1AG293700 Rh1BG258400 Rh1CG276200 Rh1DG288700 Rh7AG084400 Rh7AG084900 Rh7AG265100 Rh7BG078100 Rh7BG078600 Rh7CG085500 Rh7CG085800 Rh7CG281900 Rh7DG086500 Rh7DG086900 Rh7DG272300
rosa_wichuraiana Rw1G025990 Rw7G007440 Rw7G007460 Rw7G022700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1251
AarI CACCTGC 1 cut(s) 479
AatII GACGTC 1 cut(s) 1350
Acc36I ACCTGC 1 cut(s) 479
AccB7I CCANNNNNTGG 2 cut(s) 123, 559
AccIII TCCGGA 1 cut(s) 1369
AciI CCGC 3 cut(s) 177, 1166, 1310
AclWI GGATC 3 cut(s) 357, 667, 1380
AcoI YGGCCR 1 cut(s) 178
AcyI GRCGYC 1 cut(s) 1347
AfaI GTAC 4 cut(s) 588, 732, 1094, 1547
AfiI CCNNNNNNNGG 9 cut(s) 105, 123, 168, 169, 254, 255, 559, 979, 1166
AflIII ACRYGT 1 cut(s) 1357
AgsI TTSAA 4 cut(s) 65, 302, 593, 1472
AjnI CCWGG 2 cut(s) 291, 423
AjuI GAANNNNNNNTTGG 2 cut(s) 242, 274
AluBI AGCT 8 cut(s) 305, 697, 770, 818, 944, 1006, 1218, 1307
AluI AGCT 8 cut(s) 305, 697, 770, 818, 944, 1006, 1218, 1307
Alw26I GTCTC 1 cut(s) 982
AlwI GGATC 3 cut(s) 357, 667, 1380
AlwNI CAGNNNCTG 1 cut(s) 1307
Ama87I CYCGRG 2 cut(s) 98, 717
Aor13HI TCCGGA 1 cut(s) 1369
AoxI GGCC 7 cut(s) 95, 171, 178, 421, 597, 981, 1414
ApeKI GCWGC 7 cut(s) 706, 767, 818, 944, 1301, 1304, 1307
Asp700I GAANNNNTTC 1 cut(s) 783
AspA2I CCTAGG 1 cut(s) 1417
AspLEI GCGC 1 cut(s) 72
AspS9I GGNCC 4 cut(s) 96, 171, 1065, 1414
AsuC2I CCSGG 2 cut(s) 718, 719
AsuHPI GGTGA 3 cut(s) 295, 503, 837
AvaI CYCGRG 2 cut(s) 98, 717
AvaII GGWCC 1 cut(s) 1065
AvrII CCTAGG 1 cut(s) 1417
BauI CACGAG 1 cut(s) 344
BbsI GAAGAC 1 cut(s) 627
BbvI GCAGC 7 cut(s) 718, 779, 805, 931, 1294, 1313, 1316
BccI CCATC 4 cut(s) 716, 1229, 1459, 1536
BceAI ACGGC 2 cut(s) 682, 1070
BciT130I CCWGG 2 cut(s) 293, 425
BclI TGATCA 2 cut(s) 231, 1192
BcnI CCSGG 2 cut(s) 718, 719
BcoDI GTCTC 1 cut(s) 982
BfaI CTAG 3 cut(s) 746, 833, 1418
BfmI CTRYAG 2 cut(s) 74, 417
BfoI RGCGCY 1 cut(s) 73
BfuAI ACCTGC 1 cut(s) 479
BisI GCNGC 9 cut(s) 178, 707, 768, 819, 945, 1302, 1305, 1308, 1311
BlnI CCTAGG 1 cut(s) 1417
BlsI GCNGC 9 cut(s) 179, 708, 769, 820, 946, 1303, 1306, 1309, 1312
Bme1390I CCNGG 4 cut(s) 293, 425, 718, 719
Bme18I GGWCC 1 cut(s) 1065
BmeT110I CYCGRG 2 cut(s) 98, 717
BmgT120I GGNCC 4 cut(s) 96, 171, 1065, 1414
BmrFI CCNGG 4 cut(s) 293, 425, 718, 719
BmrI ACTGGG 2 cut(s) 400, 634
BmsI GCATC 3 cut(s) 218, 349, 1072
BmuI ACTGGG 2 cut(s) 400, 634
BoxI GACNNNNGTC 1 cut(s) 627
BpiI GAAGAC 1 cut(s) 627
BpmI CTGGAG 1 cut(s) 1186
BpuEI CTTGAG 1 cut(s) 1394
BpuMI CCSGG 2 cut(s) 718, 719
BsaAI YACGTR 1 cut(s) 1358
BsaHI GRCGYC 1 cut(s) 1347
BsaJI CCNNGG 4 cut(s) 424, 717, 937, 1417
BsaWI WCCGGW 3 cut(s) 847, 1029, 1369
Bsc4I CCNNNNNNNGG 9 cut(s) 105, 123, 168, 169, 254, 255, 559, 979, 1166
Bse118I RCCGGY 1 cut(s) 173
Bse1I ACTGG 4 cut(s) 406, 600, 629, 1510
Bse3DI GCAATG 2 cut(s) 567, 733
BseAI TCCGGA 1 cut(s) 1369
BseBI CCWGG 2 cut(s) 293, 425
BseDI CCNNGG 4 cut(s) 424, 717, 937, 1417
BseGI GGATG 4 cut(s) 613, 727, 1330, 1547
BseLI CCNNNNNNNGG 9 cut(s) 105, 123, 168, 169, 254, 255, 559, 979, 1166
BseMI GCAATG 2 cut(s) 567, 733
BseNI ACTGG 4 cut(s) 406, 600, 629, 1510
BseRI GAGGAG 2 cut(s) 43, 1009
BseXI GCAGC 7 cut(s) 718, 779, 805, 931, 1294, 1313, 1316
BsgI GTGCAG 1 cut(s) 804
BshFI GGCC 7 cut(s) 97, 173, 180, 423, 599, 983, 1416
BsiHKCI CYCGRG 2 cut(s) 98, 717
BsiSI CCGG 5 cut(s) 174, 718, 848, 1030, 1370
BslI CCNNNNNNNGG 9 cut(s) 105, 123, 168, 169, 254, 255, 559, 979, 1166
BsmAI GTCTC 1 cut(s) 982
BsmI GAATGC 2 cut(s) 546, 1408
BsnI GGCC 7 cut(s) 97, 173, 180, 423, 599, 983, 1416
BsoBI CYCGRG 2 cut(s) 98, 717
Bsp13I TCCGGA 1 cut(s) 1369
Bsp1407I TGTACA 1 cut(s) 1092
Bsp143I GATC 8 cut(s) 199, 231, 349, 659, 793, 926, 1192, 1372
Bsp19I CCATGG 1 cut(s) 937
BspACI CCGC 3 cut(s) 177, 1166, 1310
BspANI GGCC 7 cut(s) 97, 173, 180, 423, 599, 983, 1416
BspEI TCCGGA 1 cut(s) 1369
BspHI TCATGA 1 cut(s) 352
BspMAI CTGCAG 1 cut(s) 421
BspMI ACCTGC 1 cut(s) 479
BspPI GGATC 3 cut(s) 357, 667, 1380
BsrDI GCAATG 2 cut(s) 567, 733
BsrFI RCCGGY 1 cut(s) 173
BsrGI TGTACA 1 cut(s) 1092
BsrI ACTGG 4 cut(s) 406, 600, 629, 1510
BssAI RCCGGY 1 cut(s) 173
BssECI CCNNGG 4 cut(s) 424, 717, 937, 1417
BssMI GATC 8 cut(s) 199, 231, 349, 659, 793, 926, 1192, 1372
BssNI GRCGYC 1 cut(s) 1347
BssSI CACGAG 1 cut(s) 344
BssT1I CCWWGG 2 cut(s) 937, 1417
Bst2BI CACGAG 1 cut(s) 344
Bst2UI CCWGG 2 cut(s) 293, 425
Bst4CI ACNGT 4 cut(s) 78, 239, 394, 1286
BstACI GRCGYC 1 cut(s) 1347
BstAPI GCANNNNNTGC 1 cut(s) 1307
BstAUI TGTACA 1 cut(s) 1092
BstBAI YACGTR 1 cut(s) 1358
BstC8I GCNNGC 7 cut(s) 129, 175, 421, 486, 1077, 1168, 1441
BstDEI CTNAG 1 cut(s) 1392
BstDSI CCRYGG 1 cut(s) 937
BstEII GGTNACC 1 cut(s) 491
BstF5I GGATG 4 cut(s) 613, 727, 1330, 1547
BstH2I RGCGCY 1 cut(s) 73
BstHHI GCGC 1 cut(s) 72
BstKTI GATC 8 cut(s) 202, 234, 352, 662, 796, 929, 1195, 1375
BstMAI GTCTC 1 cut(s) 982
BstMBI GATC 8 cut(s) 199, 231, 349, 659, 793, 926, 1192, 1372
BstMWI GCNNNNNNNGC 4 cut(s) 703, 1307, 1310, 1449
BstNI CCWGG 2 cut(s) 293, 425
BstPAI GACNNNNGTC 1 cut(s) 627
BstPI GGTNACC 1 cut(s) 491
BstSCI CCNGG 4 cut(s) 291, 423, 716, 717
BstSFI CTRYAG 2 cut(s) 74, 417
BstV1I GCAGC 7 cut(s) 718, 779, 805, 931, 1294, 1313, 1316
BstV2I GAAGAC 1 cut(s) 627
BsuRI GGCC 7 cut(s) 97, 173, 180, 423, 599, 983, 1416
BtgI CCRYGG 1 cut(s) 937
BtsCI GGATG 4 cut(s) 613, 727, 1330, 1547
BtsI GCAGTG 1 cut(s) 229
BtsIMutI CAGTG 3 cut(s) 229, 363, 1370
BveI ACCTGC 1 cut(s) 479
Cac8I GCNNGC 7 cut(s) 129, 175, 421, 486, 1077, 1168, 1441
CaiI CAGNNNCTG 1 cut(s) 1307
CciI TCATGA 1 cut(s) 352
CfoI GCGC 1 cut(s) 72
Cfr10I RCCGGY 1 cut(s) 173
Cfr13I GGNCC 4 cut(s) 96, 171, 1065, 1414
Cfr9I CCCGGG 1 cut(s) 717
Csp6I GTAC 4 cut(s) 587, 731, 1093, 1546
CviQI GTAC 4 cut(s) 587, 731, 1093, 1546
DdeI CTNAG 1 cut(s) 1392
DpnI GATC 8 cut(s) 201, 233, 351, 661, 795, 928, 1194, 1374
DpnII GATC 8 cut(s) 199, 231, 349, 659, 793, 926, 1192, 1372
EaeI YGGCCR 1 cut(s) 178
Eco130I CCWWGG 2 cut(s) 937, 1417
Eco47I GGWCC 1 cut(s) 1065
Eco88I CYCGRG 2 cut(s) 98, 717
Eco91I GGTNACC 1 cut(s) 491
EcoO109I RGGNCCY 2 cut(s) 1065, 1414
EcoO65I GGTNACC 1 cut(s) 491
EcoRII CCWGG 2 cut(s) 291, 423
EcoT14I CCWWGG 2 cut(s) 937, 1417
EcoT22I ATGCAT 1 cut(s) 546
ErhI CCWWGG 2 cut(s) 937, 1417
FalI AAGNNNNNCTT 2 cut(s) 1477, 1509
FauI CCCGC 1 cut(s) 1159
FauNDI CATATG 2 cut(s) 1387, 1445
FbaI TGATCA 2 cut(s) 231, 1192
Fnu4HI GCNGC 9 cut(s) 178, 707, 768, 819, 945, 1302, 1305, 1308, 1311
FokI GGATG 3 cut(s) 620, 734, 1337
Fsp4HI GCNGC 9 cut(s) 178, 707, 768, 819, 945, 1302, 1305, 1308, 1311
FspBI CTAG 3 cut(s) 746, 833, 1418
GlaI GCGC 1 cut(s) 71
GluI GCNGC 9 cut(s) 178, 707, 768, 819, 945, 1302, 1305, 1308, 1311
GsuI CTGGAG 1 cut(s) 1186
HaeII RGCGCY 1 cut(s) 73
HaeIII GGCC 7 cut(s) 97, 173, 180, 423, 599, 983, 1416
HapII CCGG 5 cut(s) 174, 718, 848, 1030, 1370
HhaI GCGC 1 cut(s) 72
Hin1I GRCGYC 1 cut(s) 1347
Hin6I GCGC 1 cut(s) 70
HinP1I GCGC 1 cut(s) 70
HincII GTYRAC 1 cut(s) 1228
HindII GTYRAC 1 cut(s) 1228
HinfI GANTC 8 cut(s) 368, 525, 549, 784, 839, 863, 904, 1017
HpaII CCGG 5 cut(s) 174, 718, 848, 1030, 1370
HphI GGTGA 3 cut(s) 295, 503, 837
Hpy166II GTNNAC 3 cut(s) 1228, 1432, 1503
Hpy188I TCNGA 6 cut(s) 90, 373, 659, 664, 862, 1345
Hpy188III TCNNGA 6 cut(s) 197, 353, 1190, 1370, 1376, 1472
Hpy8I GTNNAC 3 cut(s) 1228, 1432, 1503
HpyAV CCTTC 1 cut(s) 867
HpyCH4III ACNGT 4 cut(s) 78, 239, 394, 1286
HpyCH4IV ACGT 3 cut(s) 975, 1347, 1357
HpyF10VI GCNNNNNNNGC 4 cut(s) 703, 1307, 1310, 1449
HpyF3I CTNAG 1 cut(s) 1392
HpySE526I ACGT 3 cut(s) 975, 1347, 1357
Hsp92I GRCGYC 1 cut(s) 1347
HspAI GCGC 1 cut(s) 70
Kpn2I TCCGGA 1 cut(s) 1369
KroI GCCGGC 1 cut(s) 173
KroNI GCCGGC 1 cut(s) 175
Ksp22I TGATCA 2 cut(s) 231, 1192
Kzo9I GATC 8 cut(s) 199, 231, 349, 659, 793, 926, 1192, 1372
LmnI GCTCC 3 cut(s) 941, 1223, 1449
Lsp1109I GCAGC 7 cut(s) 718, 779, 805, 931, 1294, 1313, 1316
LweI GCATC 3 cut(s) 218, 349, 1072
MaeI CTAG 3 cut(s) 746, 833, 1418
MaeII ACGT 3 cut(s) 975, 1347, 1357
MaeIII GTNAC 5 cut(s) 149, 239, 491, 843, 1181
MalI GATC 8 cut(s) 201, 233, 351, 661, 795, 928, 1194, 1374
MboI GATC 8 cut(s) 199, 231, 349, 659, 793, 926, 1192, 1372
MboII GAAGA 6 cut(s) 17, 368, 632, 703, 936, 1342
MfeI CAATTG 1 cut(s) 962
MluCI AATT 4 cut(s) 779, 956, 962, 1124
MlyI GAGTC 3 cut(s) 362, 534, 857
Mph1103I ATGCAT 1 cut(s) 546
MroI TCCGGA 1 cut(s) 1369
MroNI GCCGGC 1 cut(s) 173
MroXI GAANNNNTTC 1 cut(s) 783
MseI TTAA 3 cut(s) 288, 401, 968
MslI CAYNNNNRTG 2 cut(s) 357, 677
MspA1I CMGCKG 1 cut(s) 1307
MspI CCGG 5 cut(s) 174, 718, 848, 1030, 1370
MspR9I CCNGG 4 cut(s) 293, 425, 718, 719
MunI CAATTG 1 cut(s) 962
Mva1269I GAATGC 2 cut(s) 546, 1408
MvaI CCWGG 2 cut(s) 293, 425
MwoI GCNNNNNNNGC 4 cut(s) 703, 1307, 1310, 1449
NaeI GCCGGC 1 cut(s) 175
NciI CCSGG 2 cut(s) 718, 719
NcoI CCATGG 1 cut(s) 937
NdeI CATATG 2 cut(s) 1387, 1445
NdeII GATC 8 cut(s) 199, 231, 349, 659, 793, 926, 1192, 1372
NgoMIV GCCGGC 1 cut(s) 173
NmeAIII GCCGAG 1 cut(s) 1009
NmuCI GTSAC 3 cut(s) 149, 491, 843
NsiI ATGCAT 1 cut(s) 546
PagI TCATGA 1 cut(s) 352
PaqCI CACCTGC 1 cut(s) 479
PcsI WCGNNNNNNNCGW 1 cut(s) 981
PctI GAATGC 2 cut(s) 546, 1408
PdiI GCCGGC 1 cut(s) 175
PdmI GAANNNNTTC 1 cut(s) 783
PfeI GAWTC 5 cut(s) 549, 784, 839, 904, 1017
PflMI CCANNNNNTGG 2 cut(s) 123, 559
PkrI GCNGC 9 cut(s) 179, 708, 769, 820, 946, 1303, 1306, 1309, 1312
PleI GAGTC 3 cut(s) 362, 533, 857
PpsI GAGTC 3 cut(s) 362, 533, 857
Ppu21I YACGTR 1 cut(s) 1358
PpuMI RGGWCCY 1 cut(s) 1065
PshAI GACNNNNGTC 1 cut(s) 627
PsiI TTATAA 1 cut(s) 1251
Psp5II RGGWCCY 1 cut(s) 1065
Psp6I CCWGG 2 cut(s) 291, 423
PspEI GGTNACC 1 cut(s) 491
PspGI CCWGG 2 cut(s) 291, 423
PspPI GGNCC 4 cut(s) 96, 171, 1065, 1414
PspPPI RGGWCCY 1 cut(s) 1065
PstI CTGCAG 1 cut(s) 421
PstNI CAGNNNCTG 1 cut(s) 1307
PvuII CAGCTG 1 cut(s) 1307
RsaI GTAC 4 cut(s) 588, 732, 1094, 1547
RsaNI GTAC 4 cut(s) 587, 731, 1093, 1546
RseI CAYNNNNRTG 2 cut(s) 357, 677
SaqAI TTAA 3 cut(s) 288, 401, 968
SatI GCNGC 9 cut(s) 178, 707, 768, 819, 945, 1302, 1305, 1308, 1311
Sau3AI GATC 8 cut(s) 199, 231, 349, 659, 793, 926, 1192, 1372
Sau96I GGNCC 4 cut(s) 96, 171, 1065, 1414
SchI GAGTC 3 cut(s) 362, 534, 857
ScrFI CCNGG 4 cut(s) 293, 425, 718, 719
SfaNI GCATC 3 cut(s) 218, 349, 1072
SfcI CTRYAG 2 cut(s) 74, 417
SinI GGWCC 1 cut(s) 1065
SmaI CCCGGG 1 cut(s) 719
SmiMI CAYNNNNRTG 2 cut(s) 357, 677
SmlI CTYRAG 1 cut(s) 1409
SmoI CTYRAG 1 cut(s) 1409
Sse9I AATT 4 cut(s) 779, 956, 962, 1124
SsiI CCGC 3 cut(s) 177, 1166, 1310
SspI AATATT 2 cut(s) 445, 1255
SspMI CTAG 3 cut(s) 746, 833, 1418
StyD4I CCNGG 4 cut(s) 291, 423, 716, 717
StyI CCWWGG 2 cut(s) 937, 1417
TaaI ACNGT 4 cut(s) 78, 239, 394, 1286
TaiI ACGT 3 cut(s) 978, 1350, 1360
TaqI TCGA 1 cut(s) 212
TasI AATT 4 cut(s) 779, 956, 962, 1124
TatI WGTACW 2 cut(s) 730, 1092
TauI GCSGC 2 cut(s) 180, 1313
TfiI GAWTC 5 cut(s) 549, 784, 839, 904, 1017
Tru1I TTAA 3 cut(s) 288, 401, 968
Tru9I TTAA 3 cut(s) 288, 401, 968
TscAI CASTG 3 cut(s) 229, 370, 1370
TseFI GTSAC 3 cut(s) 149, 491, 843
TseI GCWGC 7 cut(s) 706, 767, 818, 944, 1301, 1304, 1307
Tsp45I GTSAC 3 cut(s) 149, 491, 843
TspDTI ATGAA 6 cut(s) 70, 369, 695, 917, 1415, 1550
TspGWI ACGGA 2 cut(s) 856, 908
TspMI CCCGGG 1 cut(s) 717
TspRI CASTG 3 cut(s) 229, 370, 1370
Van91I CCANNNNNTGG 2 cut(s) 123, 559
VpaK11BI GGWCC 1 cut(s) 1065
XcmI CCANNNNNNNNNTGG 1 cut(s) 251
XmaI CCCGGG 1 cut(s) 717
XmaJI CCTAGG 1 cut(s) 1417
XmnI GAANNNNTTC 1 cut(s) 783
XspI CTAG 3 cut(s) 746, 833, 1418
ZraI GACGTC 1 cut(s) 1348
Zsp2I ATGCAT 1 cut(s) 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.