Rw1G025990
BHLH Family

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000212
Physical Location & Seq
Forward (+)
52908161 .. 52909927
1767 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G025990.1

Sequence Viewer

Length: 1458 bp
ATGGATGATCTAATCATCTCCCCTTCTTCGTCTTCTTCCTTGGTTTCTCTCCCACAAGAACCAACTCTCCAACAGAGGCTGCAGTTCATACTCCAAAGCCAGCCGGAGTTGTGGTCTTACGCCATTTTCTGGCAGGCCTCAAACGACGTCGAAAATGGCCGTCTCCTTCTCGGTTGGGGAGACGGCCATTTTCAGTGCCCCAAGAACCCGTCACCCAACCACAACAACAACAACAGCCTCTCGTCCTCGGGAGGAGCGCTACACTCCGACAGAAGAAAAATCCTTAAGGGCATACAGTCCATCCTCAACGACAACAACCCGGAGCTCGACATGGACAACTTCATGGCCCTAAACGGCGATGTCTCGGACGCCGAGTGGTTCTATGTCTTGTCGTTGACTCGTTCTTTCGCCGTCGGGGACGGCTCTGTTCCCGGCAAGGCCTTCGGCTCCGGGACCTCAATCTGGCTGAGCGGGGCCCACGAGCTTCAATTCAACAACAACTGCGAGAGGGCCAAAGAGGCCCAAATTCACGGGATCGAGACCTTGATCTGCATCCCAACTTCCGACGGTGTTCTTGAAATGGGTTCCTCCGGTTTGATCAGAGAGAACTGGGGGTTGATCCAGCAGGCCAAGTCGATGTTCGGGTCGGATCAGCCCGACCCAGAAACAAGACCGCTCGAGTTCATCAACCGGAACTTCTCCTTCGCCGACATCGGCATCATTGCCGGCGTACAGGAAGAGGATCACAGCTCGTACAATGACGACAAGAAACATGTTTTTAGTTCTAGCAAGAAGAAGAACGGAGCTCCGAACCCGAACCCGGACTTCGCAGATTCCGACTGCCAGATGAAAAGAGCGCCGAAGAAACGGGGCCGGAAACCCGGGCTGGGCCGAGACACGCCGCTGAACCACGTGGAGGCGGAGCGGCAGCGGCGGGAGAAGCTCAACCACCGGTTCTACGCGCTACGCGCGGTGGTGCCCAACGTGTCGAGAATGGACAAGGCGTCGCTGCTGTCCGACGCCGTGTCGTACATCAACGACCTGAAGGCCAAGGTGGACGAGCTGGAGTCGAAGCTGCAGAGGGAGTCAAAGAAAGTCAAAGTGGAAATGGCGGACAACCAGGACAACCAGAGCACCACCACCACCGCCTCCGTGGAGCAAACACAAACAGTAAGGCCTGATATCAACAACACCAACAACGGGTCGGCTCTCGAGATTGAGGTGAAGATAGTGGGCACGGACGCCATGATTAGGGTGCAGTCGGAGAATGTGAACTACCCATCTGCGAGGTTAATGACGGCGATGCGTGACTTAGAGTTCCGCATCCACCATGCGAGCTTGTCAAGCATCAATGATCTTATGCTCCAAGATATTGTGGTGAAGGTTCCTGATAATATGAGAAGCGAAGAAGGTCTCAAAGCTGCTCTACTCGGAATATTAGATCAGAACCAAGCTTAA

Protein Analysis

485

Amino Acids

53.82

Weight (kDa)

5.49

Isoelectric Point (pI)

40.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH-MYC_N PF14215 23 - 214 1.6e-48 bHLH-MYC and R2R3-MYB transcription factors N-terminal
HLH PF00010 303 - 349 9e-12 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000212)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G00870
fragaria_vesca FvH4_5g10850 FvH4_5g10851 FvH4_5g23290 FvH4_7g17380
malus_domestica MD00G1137900.v1.1 MD01G1086800.v1.1 MD01G1086900.v1.1 MD03G1096500.v1.1 MD06G1119900.v1.1 MD06G1120000.v1.1 MD06G1120100.v1.1 MD06G1120200.v1.1 MD11G1120400.v1.1 MD11G1145000.v1.1 MD11G1254000.v1.1 MD11G1254100.v1.1 MD14G1126900.v1.1 MD14G1127000.v1.1 MD14G1137200.v1.1
prunus_persica Prupe.2G195300_v2.0.a1 Prupe.2G195300_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087600_v2.0.a1 Prupe.5G087900_v2.0.a1 Prupe.5G130300_v2.0.a1 Prupe.5G130400_v2.0.a1 Prupe.5G130500_v2.0.a1 Prupe.5G130500_v2.0.a1 Prupe.5G130600_v2.0.a1 Prupe.5G130700_v2.0.a1
pyrus_communis pycom01g09980 pycom06g11100 pycom06g11120 pycom06g11150 pycom11g10170 pycom11g22490 pycom14g11190 pycom14g11210
rosa_chinensis RchiOBHm_Chr1g0360811 RchiOBHm_Chr5g0055891 RchiOBHm_Chr6g0281801 RchiOBHm_Chr7g0187141 RchiOBHm_Chr7g0187261 RchiOBHm_Chr7g0212241 RchiOBHm_Chr7g0228701
rosa_laevigata RLG00000002910 RLG00000002911 RLG00000004796 RLG00000004799 RLG00000027784
rosa_multiflora Rmu_sc0000340.1_g000036 Rmu_sc0000378.1_g000013 Rmu_sc0000441.1_g000071 Rmu_sc0000559.1_g000036 Rmu_sc0000637.1_g000017 Rmu_sc0000765.1_g000001 Rmu_sc0000785.1_g000012 Rmu_sc0000854.1_g000018 Rmu_sc0000959.1_g000018 Rmu_sc0001018.1_g000022 Rmu_sc0001136.1_g000078 Rmu_sc0001433.1_g000037 Rmu_sc0001687.1_g000010 Rmu_sc0001716.1_g000014 Rmu_sc0001758.1_g000023 Rmu_sc0001758.1_g000031 Rmu_sc0001826.1_g000009 Rmu_sc0001850.1_g000027 Rmu_sc0001911.1_g000019 Rmu_sc0001937.1_g000001 Rmu_sc0002283.1_g000088 Rmu_sc0002532.1_g000058 Rmu_sc0002571.1_g000032 Rmu_sc0002888.1_g000014 Rmu_sc0002939.1_g000019 Rmu_sc0003197.1_g000007 Rmu_sc0003546.1_g000034 Rmu_sc0004002.1_g000003 Rmu_sc0004145.1_g000003 Rmu_sc0004301.1_g000013 Rmu_sc0004603.1_g000002 Rmu_sc0004748.1_g000007 Rmu_sc0004842.1_g000002 Rmu_sc0004990.1_g000012 Rmu_sc0005017.1_g000006 Rmu_sc0006343.1_g000001 Rmu_sc0006836.1_g000017 Rmu_sc0007491.1_g000003 Rmu_sc0008192.1_g000011 Rmu_sc0008877.1_g000007 Rmu_sc0010621.1_g000006 Rmu_sc0013354.1_g000017 Rmu_sc0013486.1_g000001 Rmu_sc0016511.1_g000001 Rmu_sc0021179.1_g000001 Rmu_sc0026563.1_g000001 Rmu_sc0027421.1_g000001 Rmu_sc0037114.1_g000001 Rmu_ssc0000022.1_g000039 Rmu_ssc0000050.1_g000048 Rmu_ssc0000141.1_g000004 Rmu_ssc0000280.1_g000002 Rmu_ssc0000387.1_g000024 Rmu_ssc0000459.1_g000030
rosa_roxburghii Rroxscaffold_3G00247010 Rroxscaffold_3G00267630 Rroxscaffold_3G00267670 Rroxscaffold_4G00295790 Rroxscaffold_4G00305930 Rroxscaffold_5G00346440 Rroxscaffold_7G00211970
rosa_rugosa Rorug01G0281800 Rorug02G0258400 Rorug04G0057400 Rorug04G0362000 Rorug04G0437300 Rorug06G0161400 Rorug06G0213300 Rorug06G0478800 Rorug06G0479300 Rorug06G0479300 Rorug07G0132200 Rorug07G0321200
rosa_samantha Rh1AG293700 Rh1BG258400 Rh1CG276200 Rh1DG288700 Rh7AG084400 Rh7AG084900 Rh7AG265100 Rh7BG078100 Rh7BG078600 Rh7CG085500 Rh7CG085800 Rh7CG281900 Rh7DG086500 Rh7DG086900 Rh7DG272300
rosa_wichuraiana Rw1G025990 Rw7G007440 Rw7G007460 Rw7G022700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 150
AccB1I GGYRCC 1 cut(s) 976
AccB7I CCANNNNNTGG 1 cut(s) 129
AccBSI CCGCTC 3 cut(s) 471, 676, 925
AccII CGCG 3 cut(s) 962, 969, 971
AclWI GGATC 4 cut(s) 542, 613, 657, 750
AcoI YGGCCR 2 cut(s) 157, 184
AcsI RAATTY 1 cut(s) 525
AcuI CTGAAG 1 cut(s) 1064
AcvI CACGTG 1 cut(s) 913
AcyI GRCGYC 5 cut(s) 147, 369, 1004, 1020, 1242
AfaI GTAC 3 cut(s) 732, 755, 1031
AfeI AGCGCT 1 cut(s) 258
AfiI CCNNNNNNNGG 7 cut(s) 129, 462, 820, 887, 916, 1200, 1251
AflII CTTAAG 1 cut(s) 284
AflIII ACRYGT 2 cut(s) 772, 984
AgeI ACCGGT 1 cut(s) 951
AgsI TTSAA 3 cut(s) 488, 493, 578
AhdI GACNNNNNGTC 2 cut(s) 1003, 1024
AjnI CCWGG 1 cut(s) 1119
AjuI GAANNNNNNNTTGG 2 cut(s) 623, 655
AloI GAACNNNNNNTCC 2 cut(s) 51, 83
Alw21I GWGCWC 3 cut(s) 327, 808, 1136
Alw26I GTCTC 6 cut(s) 167, 174, 367, 533, 888, 1418
AlwI GGATC 4 cut(s) 542, 613, 657, 750
AlwNI CAGNNNCTG 1 cut(s) 79
Ama87I CYCGRG 4 cut(s) 247, 677, 881, 1211
Aor51HI AGCGCT 1 cut(s) 258
ApaI GGGCCC 1 cut(s) 478
ApeKI GCWGC 5 cut(s) 79, 928, 1009, 1075, 1421
ApoI RAATTY 1 cut(s) 525
AsiGI ACCGGT 1 cut(s) 951
AspLEI GCGC 4 cut(s) 259, 859, 964, 971
AspS9I GGNCC 8 cut(s) 346, 453, 474, 475, 510, 520, 871, 889
AsuC2I CCSGG 6 cut(s) 320, 432, 451, 821, 882, 883
AsuHPI GGTGA 3 cut(s) 204, 1234, 1390
AvaI CYCGRG 4 cut(s) 247, 677, 881, 1211
AvaII GGWCC 1 cut(s) 453
BaeGI GKGCMC 4 cut(s) 200, 478, 981, 1238
BanI GGYRCC 1 cut(s) 976
BanII GRGCYC 3 cut(s) 327, 478, 808
BauI CACGAG 1 cut(s) 479
BbrPI CACGTG 1 cut(s) 913
BbsI GAAGAC 1 cut(s) 24
Bbv12I GWGCWC 3 cut(s) 327, 808, 1136
BbvI GCAGC 5 cut(s) 66, 940, 996, 1062, 1408
BccI CCATC 2 cut(s) 308, 1288
BceAI ACGGC 7 cut(s) 144, 199, 370, 395, 436, 1007, 1314
BcgI CGANNNNNNTGC 2 cut(s) 424, 458
BciT130I CCWGG 1 cut(s) 1121
BclI TGATCA 1 cut(s) 597
BcnI CCSGG 6 cut(s) 320, 432, 451, 821, 882, 883
BcoDI GTCTC 6 cut(s) 167, 174, 367, 533, 888, 1418
BfaI CTAG 1 cut(s) 786
BfmI CTRYAG 2 cut(s) 80, 1076
BfoI RGCGCY 2 cut(s) 260, 860
BfrI CTTAAG 1 cut(s) 284
BglI GCCNNNNNGGC 1 cut(s) 518
BisI GCNGC 8 cut(s) 80, 902, 926, 929, 932, 1010, 1076, 1422
BlpI GCTNAGC 1 cut(s) 467
BlsI GCNGC 8 cut(s) 81, 903, 927, 930, 933, 1011, 1077, 1423
Bme1390I CCNGG 7 cut(s) 320, 432, 451, 821, 882, 883, 1121
Bme18I GGWCC 1 cut(s) 453
BmeRI GACNNNNNGTC 2 cut(s) 1003, 1024
BmeT110I CYCGRG 4 cut(s) 247, 677, 881, 1211
BmgT120I GGNCC 8 cut(s) 346, 453, 474, 475, 510, 520, 871, 889
BmiI GGNNCC 8 cut(s) 448, 454, 475, 476, 586, 872, 978, 1386
BmrFI CCNGG 7 cut(s) 320, 432, 451, 821, 882, 883, 1121
BmrI ACTGGG 1 cut(s) 619
BmsI GCATC 5 cut(s) 561, 726, 1293, 1332, 1356
BmuI ACTGGG 1 cut(s) 619
BpiI GAAGAC 1 cut(s) 24
BpmI CTGGAG 1 cut(s) 1085
Bpu1102I GCTNAGC 1 cut(s) 467
BpuMI CCSGG 6 cut(s) 320, 432, 451, 821, 882, 883
BsaAI YACGTR 1 cut(s) 913
BsaBI GATNNNNATC 1 cut(s) 551
BsaHI GRCGYC 5 cut(s) 147, 369, 1004, 1020, 1242
BsaI GGTCTC 2 cut(s) 533, 1418
BsaJI CCNNGG 5 cut(s) 39, 246, 881, 1050, 1152
BsaWI WCCGGW 3 cut(s) 590, 690, 951
BsaXI ACNNNNNCTCC 4 cut(s) 51, 81, 98, 128
Bsc4I CCNNNNNNNGG 7 cut(s) 129, 462, 820, 887, 916, 1200, 1251
Bse118I RCCGGY 2 cut(s) 725, 951
Bse1I ACTGG 1 cut(s) 614
Bse3DI GCAATG 1 cut(s) 720
Bse8I GATNNNNATC 1 cut(s) 551
BseBI CCWGG 1 cut(s) 1121
BseDI CCNNGG 5 cut(s) 39, 246, 881, 1050, 1152
BseGI GGATG 4 cut(s) 10, 300, 552, 1323
BseJI GATNNNNATC 1 cut(s) 551
BseLI CCNNNNNNNGG 7 cut(s) 129, 462, 820, 887, 916, 1200, 1251
BseMI GCAATG 1 cut(s) 720
BseMII CTCAG 1 cut(s) 458
BseNI ACTGG 1 cut(s) 614
BseRI GAGGAG 1 cut(s) 267
BseSI GKGCMC 4 cut(s) 200, 478, 981, 1238
BseXI GCAGC 5 cut(s) 66, 940, 996, 1062, 1408
BseYI CCCAGC 1 cut(s) 886
BsgI GTGCAG 1 cut(s) 1277
Bsh1236I CGCG 3 cut(s) 962, 969, 971
BshNI GGYRCC 1 cut(s) 976
BshTI ACCGGT 1 cut(s) 951
BsiHKAI GWGCWC 3 cut(s) 327, 808, 1136
BsiHKCI CYCGRG 4 cut(s) 247, 677, 881, 1211
BslFI GGGAC 2 cut(s) 431, 466
BslI CCNNNNNNNGG 7 cut(s) 129, 462, 820, 887, 916, 1200, 1251
BsmAI GTCTC 6 cut(s) 167, 174, 367, 533, 888, 1418
BsmBI CGTCTC 2 cut(s) 167, 174
BsmFI GGGAC 2 cut(s) 431, 466
Bso31I GGTCTC 2 cut(s) 533, 1418
BsoBI CYCGRG 4 cut(s) 247, 677, 881, 1211
Bsp120I GGGCCC 1 cut(s) 474
Bsp1286I GDGCHC 7 cut(s) 200, 327, 478, 808, 981, 1136, 1238
Bsp143I GATC 9 cut(s) 7, 534, 546, 597, 618, 649, 742, 1354, 1441
Bsp1720I GCTNAGC 1 cut(s) 467
BspCNI CTCAG 1 cut(s) 459
BspFNI CGCG 3 cut(s) 962, 969, 971
BspLI GGNNCC 8 cut(s) 448, 454, 475, 476, 586, 872, 978, 1386
BspMAI CTGCAG 2 cut(s) 84, 1080
BspPI GGATC 4 cut(s) 542, 613, 657, 750
BspT107I GGYRCC 1 cut(s) 976
BspTI CTTAAG 1 cut(s) 284
BspTNI GGTCTC 2 cut(s) 533, 1418
BsrBI CCGCTC 3 cut(s) 471, 676, 925
BsrDI GCAATG 1 cut(s) 720
BsrFI RCCGGY 2 cut(s) 725, 951
BsrI ACTGG 1 cut(s) 614
BssAI RCCGGY 2 cut(s) 725, 951
BssECI CCNNGG 5 cut(s) 39, 246, 881, 1050, 1152
BssMI GATC 9 cut(s) 7, 534, 546, 597, 618, 649, 742, 1354, 1441
BssNI GRCGYC 5 cut(s) 147, 369, 1004, 1020, 1242
BssSI CACGAG 1 cut(s) 479
BssT1I CCWWGG 2 cut(s) 39, 1050
Bst2BI CACGAG 1 cut(s) 479
Bst2UI CCWGG 1 cut(s) 1121
Bst4CI ACNGT 3 cut(s) 297, 569, 1171
Bst6I CTCTTC 1 cut(s) 732
BstACI GRCGYC 5 cut(s) 147, 369, 1004, 1020, 1242
BstAFI CTTAAG 1 cut(s) 284
BstBAI YACGTR 1 cut(s) 913
BstC8I GCNNGC 5 cut(s) 101, 135, 627, 727, 1336
BstDEI CTNAG 2 cut(s) 467, 1312
BstDSI CCRYGG 1 cut(s) 1152
BstF5I GGATG 4 cut(s) 10, 300, 552, 1323
BstFNI CGCG 3 cut(s) 962, 969, 971
BstH2I RGCGCY 2 cut(s) 260, 860
BstHHI GCGC 4 cut(s) 259, 859, 964, 971
BstKTI GATC 9 cut(s) 10, 537, 549, 600, 621, 652, 745, 1357, 1444
BstMAI GTCTC 6 cut(s) 167, 174, 367, 533, 888, 1418
BstMBI GATC 9 cut(s) 7, 534, 546, 597, 618, 649, 742, 1354, 1441
BstMWI GCNNNNNNNGC 5 cut(s) 518, 931, 940, 968, 1344
BstNI CCWGG 1 cut(s) 1121
BstNSI RCATGY 1 cut(s) 776
BstSCI CCNGG 7 cut(s) 318, 430, 449, 819, 880, 881, 1119
BstSFI CTRYAG 2 cut(s) 80, 1076
BstSLI GKGCMC 4 cut(s) 200, 478, 981, 1238
BstUI CGCG 3 cut(s) 962, 969, 971
BstV1I GCAGC 5 cut(s) 66, 940, 996, 1062, 1408
BstV2I GAAGAC 1 cut(s) 24
BtgI CCRYGG 1 cut(s) 1152
BtgZI GCGATG 2 cut(s) 372, 1316
BtsCI GGATG 4 cut(s) 10, 300, 552, 1323
BtsIMutI CAGTG 1 cut(s) 200
Cac8I GCNNGC 5 cut(s) 101, 135, 627, 727, 1336
CaiI CAGNNNCTG 1 cut(s) 79
CfoI GCGC 4 cut(s) 259, 859, 964, 971
Cfr10I RCCGGY 2 cut(s) 725, 951
Cfr13I GGNCC 8 cut(s) 346, 453, 474, 475, 510, 520, 871, 889
Cfr9I CCCGGG 1 cut(s) 881
CseI GACGC 4 cut(s) 377, 993, 1028, 1250
Csp6I GTAC 3 cut(s) 731, 754, 1030
CspAI ACCGGT 1 cut(s) 951
CviAII CATG 5 cut(s) 331, 343, 773, 1246, 1331
CviQI GTAC 3 cut(s) 731, 754, 1030
DdeI CTNAG 2 cut(s) 467, 1312
DpnI GATC 9 cut(s) 9, 536, 548, 599, 620, 651, 744, 1356, 1443
DpnII GATC 9 cut(s) 7, 534, 546, 597, 618, 649, 742, 1354, 1441
DriI GACNNNNNGTC 2 cut(s) 1003, 1024
EaeI YGGCCR 2 cut(s) 157, 184
Eam1104I CTCTTC 1 cut(s) 732
Eam1105I GACNNNNNGTC 2 cut(s) 1003, 1024
EarI CTCTTC 1 cut(s) 732
EciI GGCGGA 2 cut(s) 935, 1127
Ecl136II GAGCTC 2 cut(s) 325, 806
Eco130I CCWWGG 2 cut(s) 39, 1050
Eco147I AGGCCT 3 cut(s) 137, 440, 1177
Eco24I GRGCYC 3 cut(s) 327, 478, 808
Eco31I GGTCTC 2 cut(s) 533, 1418
Eco32I GATATC 1 cut(s) 1183
Eco47I GGWCC 1 cut(s) 453
Eco47III AGCGCT 1 cut(s) 258
Eco53kI GAGCTC 2 cut(s) 325, 806
Eco57I CTGAAG 1 cut(s) 1064
Eco72I CACGTG 1 cut(s) 913
Eco88I CYCGRG 4 cut(s) 247, 677, 881, 1211
EcoICRI GAGCTC 2 cut(s) 325, 806
EcoO109I RGGNCCY 2 cut(s) 453, 474
EcoRII CCWGG 1 cut(s) 1119
EcoRV GATATC 1 cut(s) 1183
EcoT14I CCWWGG 2 cut(s) 39, 1050
EcoT38I GRGCYC 3 cut(s) 327, 478, 808
ErhI CCWWGG 2 cut(s) 39, 1050
Esp3I CGTCTC 2 cut(s) 167, 174
FaeI CATG 5 cut(s) 334, 346, 776, 1249, 1334
FaqI GGGAC 2 cut(s) 431, 466
FatI CATG 5 cut(s) 330, 342, 772, 1245, 1330
FauI CCCGC 2 cut(s) 464, 927
FbaI TGATCA 1 cut(s) 597
Fnu4HI GCNGC 8 cut(s) 80, 902, 926, 929, 932, 1010, 1076, 1422
FokI GGATG 4 cut(s) 17, 287, 539, 1310
FriOI GRGCYC 3 cut(s) 327, 478, 808
Fsp4HI GCNGC 8 cut(s) 80, 902, 926, 929, 932, 1010, 1076, 1422
FspBI CTAG 1 cut(s) 786
GlaI GCGC 4 cut(s) 258, 858, 963, 970
GluI GCNGC 8 cut(s) 80, 902, 926, 929, 932, 1010, 1076, 1422
GsaI CCCAGC 1 cut(s) 890
GsuI CTGGAG 1 cut(s) 1085
HaeII RGCGCY 2 cut(s) 260, 860
HgaI GACGC 4 cut(s) 377, 993, 1028, 1250
HhaI GCGC 4 cut(s) 259, 859, 964, 971
Hin1I GRCGYC 5 cut(s) 147, 369, 1004, 1020, 1242
Hin1II CATG 5 cut(s) 334, 346, 776, 1249, 1334
Hin6I GCGC 4 cut(s) 257, 857, 962, 969
HinP1I GCGC 4 cut(s) 257, 857, 962, 969
HincII GTYRAC 1 cut(s) 396
HindII GTYRAC 1 cut(s) 396
HindIII AAGCTT 1 cut(s) 1452
HinfI GANTC 4 cut(s) 397, 833, 1067, 1085
HphI GGTGA 3 cut(s) 204, 1234, 1390
Hpy166II GTNNAC 3 cut(s) 396, 1057, 1273
Hpy188III TCNNGA 7 cut(s) 249, 538, 575, 990, 1211, 1213, 1388
Hpy8I GTNNAC 3 cut(s) 396, 1057, 1273
Hpy99I CGWCG 6 cut(s) 149, 152, 416, 569, 1009, 1022
HpyAV CCTTC 7 cut(s) 33, 176, 451, 712, 1039, 1375, 1403
HpyCH4III ACNGT 3 cut(s) 297, 569, 1171
HpyCH4IV ACGT 3 cut(s) 147, 912, 984
HpyCH4V TGCA 4 cut(s) 82, 552, 1078, 1258
HpyF10VI GCNNNNNNNGC 5 cut(s) 518, 931, 940, 968, 1344
HpyF3I CTNAG 2 cut(s) 467, 1312
HpySE526I ACGT 3 cut(s) 147, 912, 984
Hsp92I GRCGYC 5 cut(s) 147, 369, 1004, 1020, 1242
Hsp92II CATG 5 cut(s) 334, 346, 776, 1249, 1334
HspAI GCGC 4 cut(s) 257, 857, 962, 969
KroI GCCGGC 1 cut(s) 725
KroNI GCCGGC 1 cut(s) 727
Ksp22I TGATCA 1 cut(s) 597
Kzo9I GATC 9 cut(s) 7, 534, 546, 597, 618, 649, 742, 1354, 1441
LmnI GCTCC 8 cut(s) 254, 322, 452, 803, 811, 922, 1156, 1368
Lsp1109I GCAGC 5 cut(s) 66, 940, 996, 1062, 1408
LweI GCATC 5 cut(s) 561, 726, 1293, 1332, 1356
MaeI CTAG 1 cut(s) 786
MaeII ACGT 3 cut(s) 147, 912, 984
MaeIII GTNAC 2 cut(s) 210, 1307
MalI GATC 9 cut(s) 9, 536, 548, 599, 620, 651, 744, 1356, 1443
MbiI CCGCTC 3 cut(s) 471, 676, 925
MboI GATC 9 cut(s) 7, 534, 546, 597, 618, 649, 742, 1354, 1441
MhlI GDGCHC 7 cut(s) 200, 327, 478, 808, 981, 1136, 1238
MluCI AATT 2 cut(s) 488, 525
MlyI GAGTC 3 cut(s) 391, 1076, 1094
MmeI TCCRAC 7 cut(s) 94, 291, 588, 627, 861, 1041, 1242
MroNI GCCGGC 1 cut(s) 725
MseI TTAA 3 cut(s) 285, 1292, 1456
MspA1I CMGCKG 2 cut(s) 904, 931
MspCI CTTAAG 1 cut(s) 284
MspR9I CCNGG 7 cut(s) 320, 432, 451, 821, 882, 883, 1121
MvaI CCWGG 1 cut(s) 1121
MvnI CGCG 3 cut(s) 962, 969, 971
MwoI GCNNNNNNNGC 5 cut(s) 518, 931, 940, 968, 1344
NaeI GCCGGC 1 cut(s) 727
NciI CCSGG 6 cut(s) 320, 432, 451, 821, 882, 883
NdeII GATC 9 cut(s) 7, 534, 546, 597, 618, 649, 742, 1354, 1441
NgoMIV GCCGGC 1 cut(s) 725
NlaIII CATG 5 cut(s) 334, 346, 776, 1249, 1334
NlaIV GGNNCC 8 cut(s) 448, 454, 475, 476, 586, 872, 978, 1386
NmeAIII GCCGAG 2 cut(s) 397, 917
NmuCI GTSAC 2 cut(s) 210, 1307
NspI RCATGY 1 cut(s) 776
PaeR7I CTCGAG 2 cut(s) 677, 1211
PceI AGGCCT 3 cut(s) 137, 440, 1177
PciI ACATGT 1 cut(s) 772
PcsI WCGNNNNNNNCGW 1 cut(s) 834
PdiI GCCGGC 1 cut(s) 727
PfeI GAWTC 1 cut(s) 833
PflMI CCANNNNNTGG 1 cut(s) 129
PfoI TCCNGGA 1 cut(s) 449
PinAI ACCGGT 1 cut(s) 951
PkrI GCNGC 8 cut(s) 81, 903, 927, 930, 933, 1011, 1077, 1423
PleI GAGTC 3 cut(s) 391, 1075, 1093
PmaCI CACGTG 1 cut(s) 913
PmlI CACGTG 1 cut(s) 913
PpsI GAGTC 3 cut(s) 391, 1075, 1093
Ppu21I YACGTR 1 cut(s) 913
PpuMI RGGWCCY 1 cut(s) 453
PscI ACATGT 1 cut(s) 772
Psp124BI GAGCTC 2 cut(s) 327, 808
Psp5II RGGWCCY 1 cut(s) 453
Psp6I CCWGG 1 cut(s) 1119
PspCI CACGTG 1 cut(s) 913
PspFI CCCAGC 1 cut(s) 886
PspGI CCWGG 1 cut(s) 1119
PspN4I GGNNCC 8 cut(s) 448, 454, 475, 476, 586, 872, 978, 1386
PspOMI GGGCCC 1 cut(s) 474
PspPI GGNCC 8 cut(s) 346, 453, 474, 475, 510, 520, 871, 889
PspPPI RGGWCCY 1 cut(s) 453
PspXI VCTCGAGB 1 cut(s) 677
PstI CTGCAG 2 cut(s) 84, 1080
PstNI CAGNNNCTG 1 cut(s) 79
RsaI GTAC 3 cut(s) 732, 755, 1031
RsaNI GTAC 3 cut(s) 731, 754, 1030
SacI GAGCTC 2 cut(s) 327, 808
SaqAI TTAA 3 cut(s) 285, 1292, 1456
SatI GCNGC 8 cut(s) 80, 902, 926, 929, 932, 1010, 1076, 1422
Sau3AI GATC 9 cut(s) 7, 534, 546, 597, 618, 649, 742, 1354, 1441
Sau96I GGNCC 8 cut(s) 346, 453, 474, 475, 510, 520, 871, 889
SchI GAGTC 3 cut(s) 391, 1076, 1094
ScrFI CCNGG 7 cut(s) 320, 432, 451, 821, 882, 883, 1121
SduI GDGCHC 7 cut(s) 200, 327, 478, 808, 981, 1136, 1238
SfaNI GCATC 5 cut(s) 561, 726, 1293, 1332, 1356
SfcI CTRYAG 2 cut(s) 80, 1076
SfiI GGCCNNNNNGGCC 1 cut(s) 518
Sfr274I CTCGAG 2 cut(s) 677, 1211
SinI GGWCC 1 cut(s) 453
SlaI CTCGAG 2 cut(s) 677, 1211
SmaI CCCGGG 1 cut(s) 883
SmlI CTYRAG 3 cut(s) 284, 677, 1211
SmoI CTYRAG 3 cut(s) 284, 677, 1211
Sse9I AATT 2 cut(s) 488, 525
SseBI AGGCCT 3 cut(s) 137, 440, 1177
SspI AATATT 1 cut(s) 1437
SspMI CTAG 1 cut(s) 786
SstI GAGCTC 2 cut(s) 327, 808
StuI AGGCCT 3 cut(s) 137, 440, 1177
StyD4I CCNGG 7 cut(s) 318, 430, 449, 819, 880, 881, 1119
StyI CCWWGG 2 cut(s) 39, 1050
TaaI ACNGT 3 cut(s) 297, 569, 1171
TaiI ACGT 3 cut(s) 150, 915, 987
TaqI TCGA 8 cut(s) 150, 327, 537, 635, 678, 989, 1070, 1212
TasI AATT 2 cut(s) 488, 525
TauI GCSGC 3 cut(s) 904, 928, 934
TfiI GAWTC 1 cut(s) 833
Tru1I TTAA 3 cut(s) 285, 1292, 1456
Tru9I TTAA 3 cut(s) 285, 1292, 1456
TscAI CASTG 1 cut(s) 200
TseFI GTSAC 2 cut(s) 210, 1307
TseI GCWGC 5 cut(s) 79, 928, 1009, 1075, 1421
Tsp45I GTSAC 2 cut(s) 210, 1307
TspDTI ATGAA 4 cut(s) 76, 331, 673, 863
TspGWI ACGGA 3 cut(s) 816, 1141, 1253
TspMI CCCGGG 1 cut(s) 881
TspRI CASTG 1 cut(s) 200
Van91I CCANNNNNTGG 1 cut(s) 129
Vha464I CTTAAG 1 cut(s) 284
VpaK11BI GGWCC 1 cut(s) 453
XapI RAATTY 1 cut(s) 525
XceI RCATGY 1 cut(s) 776
XcmI CCANNNNNNNNNTGG 1 cut(s) 1150
XhoI CTCGAG 2 cut(s) 677, 1211
XmaI CCCGGG 1 cut(s) 881
XspI CTAG 1 cut(s) 786
ZraI GACGTC 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.