FvH4_6g28031

1,4-beta-D-glucanase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000177
Physical Location & Seq
Reverse (-)
21562935 .. 21567564
4630 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g28031.t1

Sequence Viewer

Length: 717 bp
ATGGCAGGGCCTCAGTGCTGTTCTAACCCACCAATTATTAACACAAGCTGTGGTTCAGACTGTGCTGAAAACTACGGAGGCCTTAATGCCTATGTCACAGGCTCTCCTAACTCCAAGCATGCTATTCTTCTTGCCTCTGATGTTTTTGGATATGGAGCTCCCAACTTGAGGAAACTTGCAGGAAGAGTTGCTGCTTCTGGATTCTGTGTTGTAATTCCAGACTTCTTCCATGGAGATCCATTTGTGTATGACAATAACAGGCCTCTAGCAGTCTGGTTAAAGGATCATGGAACGGATAAGGGATTTGAAGATGCAAAATCAGTGATTGACGCTCTTATACGCAAAGGTTTTTCTGCAATTGGAGCAGCAGGCTTCTGTTGGGGTGCCAAGGTTGTGACTGAACTTGCAAAGTCTGAGTTCATCCAAGCTGCTGTGCTGTTGCATCCTTCATTTACTACTGTTGATGATATTGAGGGGGTTAAATCTCCGATTGCCATACTAGGGGCTGAGATTGACAACTATTCTCCACCAGAACTCGTGAAAAAGTTTGAGGATATATTAAAAGCTAGACCCGAGGTTGATAGCTATGTGAAGATATTTCCAAAAGTTGCACATGGCTGGGTTGTGAGGTATGATGCTGATGATGAAGCAGCTGCAAAGAGTTCAGAGGAAGCTCAAAATGACATGTTGCAGTGGTTTGCTAAGCATGTCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
ko00361 ko00364 ko00623 ko01100 ko01110 ko01120 ko01130 map00361 map00364 map00623 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
DLH

Protein Analysis

239

Amino Acids

25.68

Weight (kDa)

5.36

Isoelectric Point (pI)

30.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DLH PF01738 29 - 236 7.3e-28 Dienelactone hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000177)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23570 AT3G23570 AT3G23570 AT3G23600 AT3G23600
fragaria_vesca FvH4_2g23960 FvH4_2g23960 FvH4_2g23960 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14330 FvH4_6g28031 FvH4_6g29270
malus_domestica MD13G1181200.v1.1 MD13G1181300.v1.1 MD13G1181400.v1.1 MD13G1181500.v1.1 MD13G1181600.v1.1 MD13G1181700.v1.1 MD13G1181800.v1.1 MD13G1181900.v1.1 MD15G1403300.v1.1 MD15G1403400.v1.1 MD16G1181900.v1.1 MD16G1182000.v1.1 MD16G1182200.v1.1 MD16G1182300.v1.1 MD16G1182400.v1.1
prunus_persica Prupe.1G149400_v2.0.a1 Prupe.1G149600_v2.0.a1 Prupe.1G149700_v2.0.a1 Prupe.1G149800_v2.0.a1 Prupe.1G549400_v2.0.a1 Prupe.8G245600_v2.0.a1
pyrus_communis pycom13g15640 pycom13g15650 pycom13g15670 pycom13g15700 pycom15g36070 pycom16g15270 pycom16g15300 pycom16g15310
rosa_chinensis RchiOBHm_Chr1g0334461 RchiOBHm_Chr1g0334471 RchiOBHm_Chr2g0131731 RchiOBHm_Chr4g0417881 RchiOBHm_Chr4g0418561 RchiOBHm_Chr4g0418571 RchiOBHm_Chr4g0418581 RchiOBHm_Chr4g0418591 RchiOBHm_Chr4g0418601 RchiOBHm_Chr4g0418621 RchiOBHm_Chr4g0418651 RchiOBHm_Chr4g0418661 RchiOBHm_Chr4g0418671 RchiOBHm_Chr4g0418681 RchiOBHm_Chr4g0418691 RchiOBHm_Chr4g0418701 RchiOBHm_Chr4g0421371 RchiOBHm_Chr6g0291601 RchiOBHm_Chr6g0291611 RchiOBHm_Chr6g0291621 RchiOBHm_Chr6g0291631 RchiOBHm_Chr7g0229821
rosa_laevigata RLG00000007843 RLG00000007844 RLG00000007846 RLG00000007847 RLG00000007849 RLG00000012101 RLG00000012104 RLG00000029451
rosa_multiflora Rmu_sc0000363.1_g000035 Rmu_sc0000935.1_g000003 Rmu_sc0000935.1_g000010 Rmu_sc0002226.1_g000008 Rmu_sc0002226.1_g000014 Rmu_sc0002589.1_g000031 Rmu_sc0003443.1_g000009 Rmu_sc0006431.1_g000001 Rmu_sc0006431.1_g000006 Rmu_sc0006431.1_g000023 Rmu_sc0006705.1_g000014 Rmu_sc0006705.1_g000015 Rmu_sc0006705.1_g000016 Rmu_sc0006705.1_g000017 Rmu_sc0006705.1_g000018 Rmu_sc0014797.1_g000001 Rmu_sc0041885.1_g000001
rosa_roxburghii Rroxscaffold_4G00316820 Rroxscaffold_5G00361830 Rroxscaffold_5G00361840 Rroxscaffold_5G00361850 Rroxscaffold_5G00361860 Rroxscaffold_5G00361880 Rroxscaffold_5G00361890 Rroxscaffold_5G00361900 Rroxscaffold_5G00361910 Rroxscaffold_5G00361920 Rroxscaffold_5G00361930 Rroxscaffold_7G00175910 Rroxscaffold_7G00175930 Rroxscaffold_7G00175940
rosa_rugosa Rorug02G0299300 Rorug04G0154500 Rorug04G0154600 Rorug04G0154700 Rorug06G0220500
rosa_samantha Rh1AG006300 Rh1AG139200 Rh1AG139300 Rh1BG105600 Rh1CG131200 Rh1CG131300 Rh1DG143200 Rh2AG210000 Rh2CG337500 Rh2DG216200 Rh2DG376200 Rh4AG215900 Rh4AG216000 Rh4AG216100 Rh4AG216200 Rh4AG216300 Rh4AG216400 Rh4BG214300 Rh4BG214400 Rh4BG214500 Rh4BG214600 Rh4BG214700 Rh4BG214800 Rh4CG212300 Rh4CG227500 Rh4CG227700 Rh4CG227900 Rh4CG228000 Rh4CG228100 Rh4DG214200 Rh4DG215000 Rh4DG215200 Rh5AG059400 Rh6AG332900 Rh6AG333000 Rh6AG333100 Rh6BG339700 Rh6BG339900 Rh6CG346100 Rh6CG346200 Rh6DG333300 Rh6DG333400 Rh7AG435400 Rh7CG454500
rosa_wichuraiana Rw1G011480 Rw2G028580 Rw4G018270 Rw4G018280 Rw4G018290 Rw4G018360 Rw4G018390 Rw4G018400 Rw4G018410 Rw4G018420 Rw6G028910 Rw6G029000 Rw6G029010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 383
AclWI GGATC 2 cut(s) 230, 291
AfiI CCNNNNNNNGG 2 cut(s) 168, 501
AflIII ACRYGT 1 cut(s) 684
AgsI TTSAA 1 cut(s) 308
AluBI AGCT 7 cut(s) 48, 158, 428, 566, 585, 653, 674
AluI AGCT 7 cut(s) 48, 158, 428, 566, 585, 653, 674
Alw21I GWGCWC 1 cut(s) 160
AlwI GGATC 2 cut(s) 230, 291
Ama87I CYCGRG 1 cut(s) 572
AoxI GGCC 3 cut(s) 8, 79, 260
ApeKI GCWGC 5 cut(s) 191, 365, 428, 650, 653
AspS9I GGNCC 1 cut(s) 8
AvaI CYCGRG 1 cut(s) 572
BanI GGYRCC 1 cut(s) 383
BanII GRGCYC 1 cut(s) 160
BauI CACGAG 1 cut(s) 536
Bbv12I GWGCWC 1 cut(s) 160
BbvI GCAGC 5 cut(s) 178, 377, 415, 640, 662
BfaI CTAG 3 cut(s) 266, 500, 567
BisI GCNGC 5 cut(s) 192, 366, 429, 651, 654
BlpI GCTNAGC 1 cut(s) 702
BlsI GCNGC 5 cut(s) 193, 367, 430, 652, 655
BmeT110I CYCGRG 1 cut(s) 572
BmgT120I GGNCC 1 cut(s) 8
BmiI GGNNCC 1 cut(s) 385
BmsI GCATC 3 cut(s) 301, 451, 625
Bpu1102I GCTNAGC 1 cut(s) 702
BpuEI CTTGAG 1 cut(s) 187
BsaJI CCNNGG 3 cut(s) 229, 387, 573
BsaXI ACNNNNNCTCC 2 cut(s) 88, 118
Bsc4I CCNNNNNNNGG 2 cut(s) 168, 501
BseDI CCNNGG 3 cut(s) 229, 387, 573
BseGI GGATG 2 cut(s) 420, 442
BseLI CCNNNNNNNGG 2 cut(s) 168, 501
BseMII CTCAG 3 cut(s) 26, 405, 498
BseXI GCAGC 5 cut(s) 178, 377, 415, 640, 662
BseYI CCCAGC 1 cut(s) 618
BshFI GGCC 3 cut(s) 10, 81, 262
BshNI GGYRCC 1 cut(s) 383
BsiHKAI GWGCWC 1 cut(s) 160
BsiHKCI CYCGRG 1 cut(s) 572
BslI CCNNNNNNNGG 2 cut(s) 168, 501
BsnI GGCC 3 cut(s) 10, 81, 262
BsoBI CYCGRG 1 cut(s) 572
Bsp1286I GDGCHC 1 cut(s) 160
Bsp143I GATC 2 cut(s) 235, 283
Bsp1720I GCTNAGC 1 cut(s) 702
Bsp19I CCATGG 1 cut(s) 229
BspANI GGCC 3 cut(s) 10, 81, 262
BspCNI CTCAG 3 cut(s) 25, 406, 499
BspLI GGNNCC 1 cut(s) 385
BspPI GGATC 2 cut(s) 230, 291
BspT107I GGYRCC 1 cut(s) 383
BssECI CCNNGG 3 cut(s) 229, 387, 573
BssMI GATC 2 cut(s) 235, 283
BssSI CACGAG 1 cut(s) 536
BssT1I CCWWGG 2 cut(s) 229, 387
Bst2BI CACGAG 1 cut(s) 536
Bst4CI ACNGT 2 cut(s) 62, 460
Bst6I CTCTTC 1 cut(s) 178
BstC8I GCNNGC 2 cut(s) 120, 370
BstDEI CTNAG 4 cut(s) 12, 414, 507, 702
BstDSI CCRYGG 1 cut(s) 229
BstF5I GGATG 2 cut(s) 420, 442
BstKTI GATC 2 cut(s) 238, 286
BstMBI GATC 2 cut(s) 235, 283
BstMWI GCNNNNNNNGC 1 cut(s) 362
BstNSI RCATGY 3 cut(s) 122, 688, 710
BstV1I GCAGC 5 cut(s) 178, 377, 415, 640, 662
BstX2I RGATCY 1 cut(s) 235
BstYI RGATCY 1 cut(s) 235
BsuRI GGCC 3 cut(s) 10, 81, 262
BtgI CCRYGG 1 cut(s) 229
BtsCI GGATG 2 cut(s) 420, 442
BtsI GCAGTG 1 cut(s) 698
BtsIMutI CAGTG 3 cut(s) 20, 327, 698
Cac8I GCNNGC 2 cut(s) 120, 370
Cfr13I GGNCC 1 cut(s) 8
CseI GACGC 1 cut(s) 338
CviAII CATG 6 cut(s) 119, 230, 287, 614, 685, 707
DdeI CTNAG 4 cut(s) 12, 414, 507, 702
DpnI GATC 2 cut(s) 237, 285
DpnII GATC 2 cut(s) 235, 283
Eam1104I CTCTTC 1 cut(s) 178
EarI CTCTTC 1 cut(s) 178
Ecl136II GAGCTC 1 cut(s) 158
Eco130I CCWWGG 2 cut(s) 229, 387
Eco147I AGGCCT 2 cut(s) 81, 262
Eco24I GRGCYC 1 cut(s) 160
Eco53kI GAGCTC 1 cut(s) 158
Eco88I CYCGRG 1 cut(s) 572
EcoICRI GAGCTC 1 cut(s) 158
EcoO109I RGGNCCY 1 cut(s) 8
EcoT14I CCWWGG 2 cut(s) 229, 387
EcoT38I GRGCYC 1 cut(s) 160
ErhI CCWWGG 2 cut(s) 229, 387
FaeI CATG 6 cut(s) 122, 233, 290, 617, 688, 710
FatI CATG 6 cut(s) 118, 229, 286, 613, 684, 706
Fnu4HI GCNGC 5 cut(s) 192, 366, 429, 651, 654
FokI GGATG 2 cut(s) 407, 429
FriOI GRGCYC 1 cut(s) 160
Fsp4HI GCNGC 5 cut(s) 192, 366, 429, 651, 654
FspBI CTAG 3 cut(s) 266, 500, 567
GluI GCNGC 5 cut(s) 192, 366, 429, 651, 654
GsaI CCCAGC 1 cut(s) 622
HaeIII GGCC 3 cut(s) 10, 81, 262
HgaI GACGC 1 cut(s) 338
Hin1II CATG 6 cut(s) 122, 233, 290, 617, 688, 710
HinfI GANTC 1 cut(s) 201
Hpy188I TCNGA 5 cut(s) 58, 139, 415, 489, 667
Hpy188III TCNNGA 3 cut(s) 198, 218, 538
HpyAV CCTTC 1 cut(s) 456
HpyCH4III ACNGT 2 cut(s) 62, 460
HpyCH4V TGCA 8 cut(s) 179, 314, 356, 407, 442, 611, 656, 691
HpyF10VI GCNNNNNNNGC 1 cut(s) 362
HpyF3I CTNAG 4 cut(s) 12, 414, 507, 702
Hsp92II CATG 6 cut(s) 122, 233, 290, 617, 688, 710
Kzo9I GATC 2 cut(s) 235, 283
LmnI GCTCC 3 cut(s) 155, 163, 362
LpnPI CCDG 9 cut(s) 84, 165, 183, 231, 244, 259, 354, 543, 604
Lsp1109I GCAGC 5 cut(s) 178, 377, 415, 640, 662
LweI GCATC 3 cut(s) 301, 451, 625
MaeI CTAG 3 cut(s) 266, 500, 567
MaeIII GTNAC 2 cut(s) 94, 394
MalI GATC 2 cut(s) 237, 285
MboI GATC 2 cut(s) 235, 283
MboII GAAGA 5 cut(s) 119, 195, 217, 320, 604
MfeI CAATTG 1 cut(s) 357
MflI RGATCY 1 cut(s) 235
MhlI GDGCHC 1 cut(s) 160
MluCI AATT 3 cut(s) 33, 213, 357
MseI TTAA 5 cut(s) 39, 84, 278, 480, 560
MspA1I CMGCKG 1 cut(s) 653
MunI CAATTG 1 cut(s) 357
MwoI GCNNNNNNNGC 1 cut(s) 362
NcoI CCATGG 1 cut(s) 229
NdeII GATC 2 cut(s) 235, 283
NlaIII CATG 6 cut(s) 122, 233, 290, 617, 688, 710
NlaIV GGNNCC 1 cut(s) 385
NmuCI GTSAC 2 cut(s) 94, 394
NspI RCATGY 3 cut(s) 122, 688, 710
PaeI GCATGC 1 cut(s) 122
PceI AGGCCT 2 cut(s) 81, 262
PciI ACATGT 1 cut(s) 684
PfeI GAWTC 1 cut(s) 201
PkrI GCNGC 5 cut(s) 193, 367, 430, 652, 655
PscI ACATGT 1 cut(s) 684
Psp124BI GAGCTC 1 cut(s) 160
PspFI CCCAGC 1 cut(s) 618
PspN4I GGNNCC 1 cut(s) 385
PspPI GGNCC 1 cut(s) 8
PsuI RGATCY 1 cut(s) 235
PvuII CAGCTG 1 cut(s) 653
SacI GAGCTC 1 cut(s) 160
SaqAI TTAA 5 cut(s) 39, 84, 278, 480, 560
SatI GCNGC 5 cut(s) 192, 366, 429, 651, 654
Sau3AI GATC 2 cut(s) 235, 283
Sau96I GGNCC 1 cut(s) 8
SduI GDGCHC 1 cut(s) 160
SfaNI GCATC 3 cut(s) 301, 451, 625
SmlI CTYRAG 1 cut(s) 166
SmoI CTYRAG 1 cut(s) 166
SphI GCATGC 1 cut(s) 122
Sse9I AATT 3 cut(s) 33, 213, 357
SseBI AGGCCT 2 cut(s) 81, 262
SspMI CTAG 3 cut(s) 266, 500, 567
SstI GAGCTC 1 cut(s) 160
StuI AGGCCT 2 cut(s) 81, 262
StyI CCWWGG 2 cut(s) 229, 387
TaaI ACNGT 2 cut(s) 62, 460
TasI AATT 3 cut(s) 33, 213, 357
TfiI GAWTC 1 cut(s) 201
Tru1I TTAA 5 cut(s) 39, 84, 278, 480, 560
Tru9I TTAA 5 cut(s) 39, 84, 278, 480, 560
TscAI CASTG 3 cut(s) 20, 327, 698
TseFI GTSAC 2 cut(s) 94, 394
TseI GCWGC 5 cut(s) 191, 365, 428, 650, 653
Tsp45I GTSAC 2 cut(s) 94, 394
TspDTI ATGAA 3 cut(s) 409, 438, 660
TspGWI ACGGA 2 cut(s) 90, 308
TspRI CASTG 3 cut(s) 20, 327, 698
XceI RCATGY 3 cut(s) 122, 688, 710
XspI CTAG 3 cut(s) 266, 500, 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.