MD13G1181400.v1.1

1,4-beta-D-glucanase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0000177
Physical Location & Seq
Reverse (-)
15099464 .. 15102502
3039 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1181400.v1.1.491

Sequence Viewer

Length: 723 bp
ATGTCAGGCCCTCAGTGCTGCTCCCACCCACCAACCCTGAACCCATCGAGTGGCTCCGGCCACGTTGAGAAGTTAGGCGGTCTCGACACCTATCTGGTCGGCTCCCCGGACTCCAAGGTCGCCGTTCTTCTCGTCTCTGATGTTTTTGGATACGATGCTCCAAACTTGAGGAAGCTGGCAGACAAACTTGCAGCTGGTGGTTTTTTTGTTGTGATCCCTGACTTCTTTTACGGAGACCCTTTTGTACTTGGAGAGGAGGGATTTGCCGCTTTCCCTGGCTGGATAAAACATCATGGAACGGACAAAGCATTAGAAGATGCAAAACCAATACTTGAAGCTTTAAAAAGTAAAGGTATTTCTGCAGTTGGTGCTGTAGGATTCTGTTATGGGGCCAAGGTTGTGGTTGAACTTTCAAAGCATGACTTTATCCAAGCTGCTGCTATGTGTCATCCTTCATTTGTCACTCTGGATGATATCAAGGCGGTTAAGGTTCCCACTCAAATACTTGGCGCTGAGATTGATAATATGTCTCCACCTGAAGTCGTGAAACAATTTGAGGAGGCTTTAGCTGCAAAACCTGAGATCAAAAGCCGTGTGAAGATATTTCCAAAAGTGGAACACGGGTGGACCACTAGGTACAACGATGAAGATGAAGCAGCTTGTAAGGCTGCCGAGGAGGCTCATCAAGACTTGTTGGAGTGGTTCTTGAACCATGTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
ko00361 ko00364 ko00623 ko01100 ko01110 ko01120 ko01130 map00361 map00364 map00623 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
DLH

Protein Analysis

241

Amino Acids

25.92

Weight (kDa)

5.34

Isoelectric Point (pI)

30.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DLH PF01738 28 - 237 9e-32 Dienelactone hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000177)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23570 AT3G23570 AT3G23570 AT3G23600 AT3G23600
fragaria_vesca FvH4_2g23960 FvH4_2g23960 FvH4_2g23960 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14330 FvH4_6g28031 FvH4_6g29270
malus_domestica MD13G1181200.v1.1 MD13G1181300.v1.1 MD13G1181400.v1.1 MD13G1181500.v1.1 MD13G1181600.v1.1 MD13G1181700.v1.1 MD13G1181800.v1.1 MD13G1181900.v1.1 MD15G1403300.v1.1 MD15G1403400.v1.1 MD16G1181900.v1.1 MD16G1182000.v1.1 MD16G1182200.v1.1 MD16G1182300.v1.1 MD16G1182400.v1.1
prunus_persica Prupe.1G149400_v2.0.a1 Prupe.1G149600_v2.0.a1 Prupe.1G149700_v2.0.a1 Prupe.1G149800_v2.0.a1 Prupe.1G549400_v2.0.a1 Prupe.8G245600_v2.0.a1
pyrus_communis pycom13g15640 pycom13g15650 pycom13g15670 pycom13g15700 pycom15g36070 pycom16g15270 pycom16g15300 pycom16g15310
rosa_chinensis RchiOBHm_Chr1g0334461 RchiOBHm_Chr1g0334471 RchiOBHm_Chr2g0131731 RchiOBHm_Chr4g0417881 RchiOBHm_Chr4g0418561 RchiOBHm_Chr4g0418571 RchiOBHm_Chr4g0418581 RchiOBHm_Chr4g0418591 RchiOBHm_Chr4g0418601 RchiOBHm_Chr4g0418621 RchiOBHm_Chr4g0418651 RchiOBHm_Chr4g0418661 RchiOBHm_Chr4g0418671 RchiOBHm_Chr4g0418681 RchiOBHm_Chr4g0418691 RchiOBHm_Chr4g0418701 RchiOBHm_Chr4g0421371 RchiOBHm_Chr6g0291601 RchiOBHm_Chr6g0291611 RchiOBHm_Chr6g0291621 RchiOBHm_Chr6g0291631 RchiOBHm_Chr7g0229821
rosa_laevigata RLG00000007843 RLG00000007844 RLG00000007846 RLG00000007847 RLG00000007849 RLG00000012101 RLG00000012104 RLG00000029451
rosa_multiflora Rmu_sc0000363.1_g000035 Rmu_sc0000935.1_g000003 Rmu_sc0000935.1_g000010 Rmu_sc0002226.1_g000008 Rmu_sc0002226.1_g000014 Rmu_sc0002589.1_g000031 Rmu_sc0003443.1_g000009 Rmu_sc0006431.1_g000001 Rmu_sc0006431.1_g000006 Rmu_sc0006431.1_g000023 Rmu_sc0006705.1_g000014 Rmu_sc0006705.1_g000015 Rmu_sc0006705.1_g000016 Rmu_sc0006705.1_g000017 Rmu_sc0006705.1_g000018 Rmu_sc0014797.1_g000001 Rmu_sc0041885.1_g000001
rosa_roxburghii Rroxscaffold_4G00316820 Rroxscaffold_5G00361830 Rroxscaffold_5G00361840 Rroxscaffold_5G00361850 Rroxscaffold_5G00361860 Rroxscaffold_5G00361880 Rroxscaffold_5G00361890 Rroxscaffold_5G00361900 Rroxscaffold_5G00361910 Rroxscaffold_5G00361920 Rroxscaffold_5G00361930 Rroxscaffold_7G00175910 Rroxscaffold_7G00175930 Rroxscaffold_7G00175940
rosa_rugosa Rorug02G0299300 Rorug04G0154500 Rorug04G0154600 Rorug04G0154700 Rorug06G0220500
rosa_samantha Rh1AG006300 Rh1AG139200 Rh1AG139300 Rh1BG105600 Rh1CG131200 Rh1CG131300 Rh1DG143200 Rh2AG210000 Rh2CG337500 Rh2DG216200 Rh2DG376200 Rh4AG215900 Rh4AG216000 Rh4AG216100 Rh4AG216200 Rh4AG216300 Rh4AG216400 Rh4BG214300 Rh4BG214400 Rh4BG214500 Rh4BG214600 Rh4BG214700 Rh4BG214800 Rh4CG212300 Rh4CG227500 Rh4CG227700 Rh4CG227900 Rh4CG228000 Rh4CG228100 Rh4DG214200 Rh4DG215000 Rh4DG215200 Rh5AG059400 Rh6AG332900 Rh6AG333000 Rh6AG333100 Rh6BG339700 Rh6BG339900 Rh6CG346100 Rh6CG346200 Rh6DG333300 Rh6DG333400 Rh7AG435400 Rh7CG454500
rosa_wichuraiana Rw1G011480 Rw2G028580 Rw4G018270 Rw4G018280 Rw4G018290 Rw4G018360 Rw4G018390 Rw4G018400 Rw4G018410 Rw4G018420 Rw6G028910 Rw6G029000 Rw6G029010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 116
AccB7I CCANNNNNTGG 1 cut(s) 50
AciI CCGC 3 cut(s) 78, 267, 482
AclWI GGATC 1 cut(s) 208
AcoI YGGCCR 1 cut(s) 58
AcuI CTGAAG 1 cut(s) 558
AfaI GTAC 2 cut(s) 246, 638
AfiI CCNNNNNNNGG 1 cut(s) 50
AgsI TTSAA 4 cut(s) 335, 407, 414, 709
AjnI CCWGG 1 cut(s) 274
AluBI AGCT 6 cut(s) 175, 194, 338, 434, 569, 659
AluI AGCT 6 cut(s) 175, 194, 338, 434, 569, 659
Alw26I GTCTC 4 cut(s) 86, 139, 228, 534
AlwI GGATC 1 cut(s) 208
AoxI GGCC 3 cut(s) 7, 58, 390
ApeKI GCWGC 7 cut(s) 18, 191, 434, 437, 569, 656, 668
AspLEI GCGC 1 cut(s) 512
AspS9I GGNCC 3 cut(s) 8, 390, 627
AsuC2I CCSGG 1 cut(s) 107
AvaII GGWCC 1 cut(s) 627
BbvI GCAGC 7 cut(s) 5, 203, 421, 424, 556, 655, 668
BccI CCATC 1 cut(s) 52
BceAI ACGGC 2 cut(s) 107, 576
BciT130I CCWGG 1 cut(s) 276
BciVI GTATCC 1 cut(s) 143
BcnI CCSGG 1 cut(s) 107
BcoDI GTCTC 4 cut(s) 86, 139, 228, 534
BfaI CTAG 1 cut(s) 633
BfmI CTRYAG 2 cut(s) 360, 372
BfoI RGCGCY 1 cut(s) 513
BfuI GTATCC 1 cut(s) 143
BglI GCCNNNNNGGC 1 cut(s) 677
BisI GCNGC 8 cut(s) 19, 192, 267, 435, 438, 570, 657, 669
BlsI GCNGC 8 cut(s) 20, 193, 268, 436, 439, 571, 658, 670
Bme1390I CCNGG 2 cut(s) 107, 276
Bme18I GGWCC 1 cut(s) 627
BmgT120I GGNCC 3 cut(s) 8, 390, 627
BmiI GGNNCC 4 cut(s) 55, 103, 391, 492
BmrFI CCNGG 2 cut(s) 107, 276
BmsI GCATC 2 cut(s) 145, 307
BpuEI CTTGAG 1 cut(s) 187
BpuMI CCSGG 1 cut(s) 107
BsaI GGTCTC 2 cut(s) 86, 228
BsaJI CCNNGG 5 cut(s) 105, 114, 274, 393, 672
Bsc4I CCNNNNNNNGG 1 cut(s) 50
BseBI CCWGG 1 cut(s) 276
BseDI CCNNGG 5 cut(s) 105, 114, 274, 393, 672
BseGI GGATG 2 cut(s) 448, 475
BseLI CCNNNNNNNGG 1 cut(s) 50
BseMII CTCAG 3 cut(s) 26, 504, 570
BseRI GAGGAG 3 cut(s) 269, 572, 689
BseXI GCAGC 7 cut(s) 5, 203, 421, 424, 556, 655, 668
BshFI GGCC 3 cut(s) 9, 60, 392
BsiSI CCGG 2 cut(s) 57, 107
BslI CCNNNNNNNGG 1 cut(s) 50
BsmAI GTCTC 4 cut(s) 86, 139, 228, 534
BsmBI CGTCTC 1 cut(s) 139
BsnI GGCC 3 cut(s) 9, 60, 392
Bso31I GGTCTC 2 cut(s) 86, 228
Bsp143I GATC 2 cut(s) 213, 582
BspACI CCGC 3 cut(s) 78, 267, 482
BspANI GGCC 3 cut(s) 9, 60, 392
BspCNI CTCAG 3 cut(s) 25, 505, 571
BspLI GGNNCC 4 cut(s) 55, 103, 391, 492
BspMAI CTGCAG 1 cut(s) 364
BspPI GGATC 1 cut(s) 208
BspTNI GGTCTC 2 cut(s) 86, 228
BssECI CCNNGG 5 cut(s) 105, 114, 274, 393, 672
BssMI GATC 2 cut(s) 213, 582
BssT1I CCWWGG 2 cut(s) 114, 393
Bst2UI CCWGG 1 cut(s) 276
BstAPI GCANNNNNTGC 1 cut(s) 368
BstC8I GCNNGC 1 cut(s) 177
BstDEI CTNAG 3 cut(s) 12, 513, 579
BstF5I GGATG 2 cut(s) 448, 475
BstH2I RGCGCY 1 cut(s) 513
BstHHI GCGC 1 cut(s) 512
BstKTI GATC 2 cut(s) 216, 585
BstMAI GTCTC 4 cut(s) 86, 139, 228, 534
BstMBI GATC 2 cut(s) 213, 582
BstMWI GCNNNNNNNGC 5 cut(s) 15, 368, 569, 665, 677
BstNI CCWGG 1 cut(s) 276
BstSCI CCNGG 2 cut(s) 105, 274
BstSFI CTRYAG 2 cut(s) 360, 372
BstV1I GCAGC 7 cut(s) 5, 203, 421, 424, 556, 655, 668
BstXI CCANNNNNNTGG 1 cut(s) 400
BsuI GTATCC 1 cut(s) 143
BsuRI GGCC 3 cut(s) 9, 60, 392
BtsCI GGATG 2 cut(s) 448, 475
BtsIMutI CAGTG 1 cut(s) 20
Cac8I GCNNGC 1 cut(s) 177
CfoI GCGC 1 cut(s) 512
Cfr13I GGNCC 3 cut(s) 8, 390, 627
Csp6I GTAC 2 cut(s) 245, 637
CviAII CATG 3 cut(s) 293, 419, 713
CviQI GTAC 2 cut(s) 245, 637
DdeI CTNAG 3 cut(s) 12, 513, 579
DpnI GATC 2 cut(s) 215, 584
DpnII GATC 2 cut(s) 213, 582
DraI TTTAAA 1 cut(s) 342
DrdI GACNNNNNNGTC 1 cut(s) 116
DseDI GACNNNNNNGTC 1 cut(s) 116
EaeI YGGCCR 1 cut(s) 58
Eco130I CCWWGG 2 cut(s) 114, 393
Eco31I GGTCTC 2 cut(s) 86, 228
Eco32I GATATC 1 cut(s) 475
Eco47I GGWCC 1 cut(s) 627
Eco57I CTGAAG 1 cut(s) 558
EcoO109I RGGNCCY 1 cut(s) 8
EcoRII CCWGG 1 cut(s) 274
EcoRV GATATC 1 cut(s) 475
EcoT14I CCWWGG 2 cut(s) 114, 393
ErhI CCWWGG 2 cut(s) 114, 393
Esp3I CGTCTC 1 cut(s) 139
FaeI CATG 3 cut(s) 296, 422, 716
FaiI YATR 6 cut(s) 294, 387, 420, 443, 527, 714
FalI AAGNNNNNCTT 2 cut(s) 407, 439
FatI CATG 3 cut(s) 292, 418, 712
Fnu4HI GCNGC 8 cut(s) 19, 192, 267, 435, 438, 570, 657, 669
FokI GGATG 2 cut(s) 435, 482
Fsp4HI GCNGC 8 cut(s) 19, 192, 267, 435, 438, 570, 657, 669
FspBI CTAG 1 cut(s) 633
GlaI GCGC 1 cut(s) 511
GluI GCNGC 8 cut(s) 19, 192, 267, 435, 438, 570, 657, 669
HaeII RGCGCY 1 cut(s) 513
HaeIII GGCC 3 cut(s) 9, 60, 392
HapII CCGG 2 cut(s) 57, 107
HhaI GCGC 1 cut(s) 512
Hin1II CATG 3 cut(s) 296, 422, 716
Hin6I GCGC 1 cut(s) 510
HinP1I GCGC 1 cut(s) 510
HindIII AAGCTT 1 cut(s) 336
HinfI GANTC 2 cut(s) 110, 378
HpaII CCGG 2 cut(s) 57, 107
Hpy166II GTNNAC 1 cut(s) 627
Hpy188I TCNGA 1 cut(s) 139
Hpy188III TCNNGA 5 cut(s) 83, 467, 544, 686, 706
Hpy8I GTNNAC 1 cut(s) 627
HpyAV CCTTC 1 cut(s) 462
HpyCH4IV ACGT 1 cut(s) 63
HpyCH4V TGCA 4 cut(s) 191, 320, 362, 572
HpyF10VI GCNNNNNNNGC 5 cut(s) 15, 368, 569, 665, 677
HpyF3I CTNAG 3 cut(s) 12, 513, 579
HpySE526I ACGT 1 cut(s) 63
Hsp92II CATG 3 cut(s) 296, 422, 716
HspAI GCGC 1 cut(s) 510
Kzo9I GATC 2 cut(s) 213, 582
LmnI GCTCC 4 cut(s) 26, 59, 107, 163
Lsp1109I GCAGC 7 cut(s) 5, 203, 421, 424, 556, 655, 668
LweI GCATC 2 cut(s) 145, 307
MaeI CTAG 1 cut(s) 633
MaeII ACGT 1 cut(s) 63
MaeIII GTNAC 1 cut(s) 460
MalI GATC 2 cut(s) 215, 584
MboI GATC 2 cut(s) 213, 582
MboII GAAGA 4 cut(s) 119, 326, 610, 659
MluCI AATT 1 cut(s) 551
MlyI GAGTC 1 cut(s) 104
MmeI TCCRAC 1 cut(s) 675
MnlI CCTC 8 cut(s) 21, 162, 247, 250, 550, 553, 667, 670
MseI TTAA 2 cut(s) 341, 486
MspA1I CMGCKG 1 cut(s) 194
MspI CCGG 2 cut(s) 57, 107
MspR9I CCNGG 2 cut(s) 107, 276
MvaI CCWGG 1 cut(s) 276
MwoI GCNNNNNNNGC 5 cut(s) 15, 368, 569, 665, 677
NciI CCSGG 1 cut(s) 107
NdeII GATC 2 cut(s) 213, 582
NlaIII CATG 3 cut(s) 296, 422, 716
NlaIV GGNNCC 4 cut(s) 55, 103, 391, 492
NmeAIII GCCGAG 1 cut(s) 697
NmuCI GTSAC 1 cut(s) 460
PfeI GAWTC 1 cut(s) 378
PflMI CCANNNNNTGG 1 cut(s) 50
PkrI GCNGC 8 cut(s) 20, 193, 268, 436, 439, 571, 658, 670
PleI GAGTC 1 cut(s) 104
PpsI GAGTC 1 cut(s) 104
Psp6I CCWGG 1 cut(s) 274
PspGI CCWGG 1 cut(s) 274
PspN4I GGNNCC 4 cut(s) 55, 103, 391, 492
PspPI GGNCC 3 cut(s) 8, 390, 627
PstI CTGCAG 1 cut(s) 364
PvuII CAGCTG 1 cut(s) 194
RsaI GTAC 2 cut(s) 246, 638
RsaNI GTAC 2 cut(s) 245, 637
SaqAI TTAA 2 cut(s) 341, 486
SatI GCNGC 8 cut(s) 19, 192, 267, 435, 438, 570, 657, 669
Sau3AI GATC 2 cut(s) 213, 582
Sau96I GGNCC 3 cut(s) 8, 390, 627
SchI GAGTC 1 cut(s) 104
ScrFI CCNGG 2 cut(s) 107, 276
SfaNI GCATC 2 cut(s) 145, 307
SfcI CTRYAG 2 cut(s) 360, 372
SinI GGWCC 1 cut(s) 627
SmlI CTYRAG 1 cut(s) 166
SmoI CTYRAG 1 cut(s) 166
Sse9I AATT 1 cut(s) 551
SsiI CCGC 3 cut(s) 78, 267, 482
SspMI CTAG 1 cut(s) 633
StyD4I CCNGG 2 cut(s) 105, 274
StyI CCWWGG 2 cut(s) 114, 393
TaiI ACGT 1 cut(s) 66
TaqI TCGA 2 cut(s) 47, 84
TasI AATT 1 cut(s) 551
TatI WGTACW 1 cut(s) 244
TauI GCSGC 1 cut(s) 269
TfiI GAWTC 1 cut(s) 378
Tru1I TTAA 2 cut(s) 341, 486
Tru9I TTAA 2 cut(s) 341, 486
TscAI CASTG 1 cut(s) 20
TseFI GTSAC 1 cut(s) 460
TseI GCWGC 7 cut(s) 18, 191, 434, 437, 569, 656, 668
Tsp45I GTSAC 1 cut(s) 460
TspDTI ATGAA 3 cut(s) 444, 660, 666
TspGWI ACGGA 2 cut(s) 246, 314
TspRI CASTG 1 cut(s) 20
Van91I CCANNNNNTGG 1 cut(s) 50
VpaK11BI GGWCC 1 cut(s) 627
XspI CTAG 1 cut(s) 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.