pycom16g15270

1,4-beta-D-glucanase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0000177
Physical Location & Seq
Reverse (-)
11227903 .. 11230344
2442 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g15270.2

Sequence Viewer

Length: 618 bp
ATGTCACTGGCTCCCCTGACTCCAAGCTTGCCGTTCTTCTCGTCCATGACGCTTTTGGAAGCTCCAAAGGCTAGGATGCTTGCAGACAGAATTGCAGCTGCTGGGTTCATTGTTGTGCTCCCGGACTTCTTCAATCGAGACCCTTTTAATGGGGACCTTGCCTCTCTACCGGTTTGGATAAAAGCTCATGCACCGGACAAAACAATCGAAGATGCAAAACTGGTGATTGAAGCTTTAAAAAGTAAAGGTGTTTCTGCAATAGGTGCTGTAGGCTTCTGTTGGGGCGGCAAGGGTGTCGTTGAACTTGCGAAGCATGACTTTATCCAAGCTGCTGTTTTGTGTCATCCTTCATTTGTCACTCTGGATGATATCAAGGCGGTTAAGGTTCCGATTTCTGTACTTGGAGCTGAGATTGATCAGCTGACTCCACCCGAAGTCGTCAAACAATTTGAAGAGGTTTTAACTGCAAAACCTGAGATTAAAAGCCATGTGAAGATATTCCCGAAGCTCGTACATGGGTGGACGTTGAGGTACAACGATGAAGACGAGGCGGCTGTCAAGGCAGCTGAGGAGGCTCATCAAGACTTGTTGGGGTGGTTCTTGGAGCATATCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
ko00361 ko00364 ko00623 ko01100 ko01110 ko01120 ko01130 map00361 map00364 map00623 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
DLH

Protein Analysis

206

Amino Acids

22.4

Weight (kDa)

5.63

Isoelectric Point (pI)

33.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000177)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23570 AT3G23570 AT3G23570 AT3G23600 AT3G23600
fragaria_vesca FvH4_2g23960 FvH4_2g23960 FvH4_2g23960 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14330 FvH4_6g28031 FvH4_6g29270
malus_domestica MD13G1181200.v1.1 MD13G1181300.v1.1 MD13G1181400.v1.1 MD13G1181500.v1.1 MD13G1181600.v1.1 MD13G1181700.v1.1 MD13G1181800.v1.1 MD13G1181900.v1.1 MD15G1403300.v1.1 MD15G1403400.v1.1 MD16G1181900.v1.1 MD16G1182000.v1.1 MD16G1182200.v1.1 MD16G1182300.v1.1 MD16G1182400.v1.1
prunus_persica Prupe.1G149400_v2.0.a1 Prupe.1G149600_v2.0.a1 Prupe.1G149700_v2.0.a1 Prupe.1G149800_v2.0.a1 Prupe.1G549400_v2.0.a1 Prupe.8G245600_v2.0.a1
pyrus_communis pycom13g15640 pycom13g15650 pycom13g15670 pycom13g15700 pycom15g36070 pycom16g15270 pycom16g15300 pycom16g15310
rosa_chinensis RchiOBHm_Chr1g0334461 RchiOBHm_Chr1g0334471 RchiOBHm_Chr2g0131731 RchiOBHm_Chr4g0417881 RchiOBHm_Chr4g0418561 RchiOBHm_Chr4g0418571 RchiOBHm_Chr4g0418581 RchiOBHm_Chr4g0418591 RchiOBHm_Chr4g0418601 RchiOBHm_Chr4g0418621 RchiOBHm_Chr4g0418651 RchiOBHm_Chr4g0418661 RchiOBHm_Chr4g0418671 RchiOBHm_Chr4g0418681 RchiOBHm_Chr4g0418691 RchiOBHm_Chr4g0418701 RchiOBHm_Chr4g0421371 RchiOBHm_Chr6g0291601 RchiOBHm_Chr6g0291611 RchiOBHm_Chr6g0291621 RchiOBHm_Chr6g0291631 RchiOBHm_Chr7g0229821
rosa_laevigata RLG00000007843 RLG00000007844 RLG00000007846 RLG00000007847 RLG00000007849 RLG00000012101 RLG00000012104 RLG00000029451
rosa_multiflora Rmu_sc0000363.1_g000035 Rmu_sc0000935.1_g000003 Rmu_sc0000935.1_g000010 Rmu_sc0002226.1_g000008 Rmu_sc0002226.1_g000014 Rmu_sc0002589.1_g000031 Rmu_sc0003443.1_g000009 Rmu_sc0006431.1_g000001 Rmu_sc0006431.1_g000006 Rmu_sc0006431.1_g000023 Rmu_sc0006705.1_g000014 Rmu_sc0006705.1_g000015 Rmu_sc0006705.1_g000016 Rmu_sc0006705.1_g000017 Rmu_sc0006705.1_g000018 Rmu_sc0014797.1_g000001 Rmu_sc0041885.1_g000001
rosa_roxburghii Rroxscaffold_4G00316820 Rroxscaffold_5G00361830 Rroxscaffold_5G00361840 Rroxscaffold_5G00361850 Rroxscaffold_5G00361860 Rroxscaffold_5G00361880 Rroxscaffold_5G00361890 Rroxscaffold_5G00361900 Rroxscaffold_5G00361910 Rroxscaffold_5G00361920 Rroxscaffold_5G00361930 Rroxscaffold_7G00175910 Rroxscaffold_7G00175930 Rroxscaffold_7G00175940
rosa_rugosa Rorug02G0299300 Rorug04G0154500 Rorug04G0154600 Rorug04G0154700 Rorug06G0220500
rosa_samantha Rh1AG006300 Rh1AG139200 Rh1AG139300 Rh1BG105600 Rh1CG131200 Rh1CG131300 Rh1DG143200 Rh2AG210000 Rh2CG337500 Rh2DG216200 Rh2DG376200 Rh4AG215900 Rh4AG216000 Rh4AG216100 Rh4AG216200 Rh4AG216300 Rh4AG216400 Rh4BG214300 Rh4BG214400 Rh4BG214500 Rh4BG214600 Rh4BG214700 Rh4BG214800 Rh4CG212300 Rh4CG227500 Rh4CG227700 Rh4CG227900 Rh4CG228000 Rh4CG228100 Rh4DG214200 Rh4DG215000 Rh4DG215200 Rh5AG059400 Rh6AG332900 Rh6AG333000 Rh6AG333100 Rh6BG339700 Rh6BG339900 Rh6CG346100 Rh6CG346200 Rh6DG333300 Rh6DG333400 Rh7AG435400 Rh7CG454500
rosa_wichuraiana Rw1G011480 Rw2G028580 Rw4G018270 Rw4G018280 Rw4G018290 Rw4G018360 Rw4G018390 Rw4G018400 Rw4G018410 Rw4G018420 Rw6G028910 Rw6G029000 Rw6G029010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 285, 377, 551
AfaI GTAC 3 cut(s) 399, 513, 533
AfiI CCNNNNNNNGG 1 cut(s) 149
AgeI ACCGGT 1 cut(s) 169
AgsI TTSAA 4 cut(s) 133, 230, 302, 452
Alw21I GWGCWC 1 cut(s) 120
Alw26I GTCTC 1 cut(s) 132
AlwNI CAGNNNCTG 1 cut(s) 101
ApeKI GCWGC 4 cut(s) 95, 98, 329, 563
AsiGI ACCGGT 1 cut(s) 169
Asp700I GAANNNNTTC 1 cut(s) 497
AspS9I GGNCC 1 cut(s) 154
AsuC2I CCSGG 1 cut(s) 122
AsuHPI GGTGA 1 cut(s) 235
AvaII GGWCC 1 cut(s) 154
BbsI GAAGAC 1 cut(s) 549
Bbv12I GWGCWC 1 cut(s) 120
BbvCI CCTCAGC 1 cut(s) 567
BbvI GCAGC 4 cut(s) 85, 107, 316, 575
BceAI ACGGC 1 cut(s) 16
BcgI CGANNNNNNTGC 2 cut(s) 277, 311
BclI TGATCA 1 cut(s) 415
BcnI CCSGG 1 cut(s) 122
BcoDI GTCTC 1 cut(s) 132
BfaI CTAG 1 cut(s) 72
BfmI CTRYAG 1 cut(s) 267
BisI GCNGC 6 cut(s) 96, 99, 286, 330, 552, 564
BlsI GCNGC 6 cut(s) 97, 100, 287, 331, 553, 565
Bme1390I CCNGG 1 cut(s) 122
Bme18I GGWCC 1 cut(s) 154
BmgT120I GGNCC 1 cut(s) 154
BmiI GGNNCC 3 cut(s) 12, 155, 387
BmrFI CCNGG 1 cut(s) 122
BmsI GCATC 2 cut(s) 66, 202
BpiI GAAGAC 1 cut(s) 549
Bpu10I CCTNAGC 1 cut(s) 567
BpuMI CCSGG 1 cut(s) 122
BsaI GGTCTC 1 cut(s) 132
BsaWI WCCGGW 2 cut(s) 169, 193
Bsc4I CCNNNNNNNGG 1 cut(s) 149
Bse118I RCCGGY 1 cut(s) 169
Bse1I ACTGG 2 cut(s) 12, 225
BseGI GGATG 3 cut(s) 81, 343, 370
BseLI CCNNNNNNNGG 1 cut(s) 149
BseMII CTCAG 3 cut(s) 399, 465, 558
BseNI ACTGG 2 cut(s) 12, 225
BseRI GAGGAG 1 cut(s) 584
BseXI GCAGC 4 cut(s) 85, 107, 316, 575
BseYI CCCAGC 1 cut(s) 101
BshTI ACCGGT 1 cut(s) 169
BsiHKAI GWGCWC 1 cut(s) 120
BsiSI CCGG 3 cut(s) 122, 170, 194
BslFI GGGAC 1 cut(s) 167
BslI CCNNNNNNNGG 1 cut(s) 149
BsmAI GTCTC 1 cut(s) 132
BsmFI GGGAC 1 cut(s) 167
Bso31I GGTCTC 1 cut(s) 132
Bsp1286I GDGCHC 1 cut(s) 120
Bsp143I GATC 1 cut(s) 415
BspACI CCGC 3 cut(s) 285, 377, 551
BspCNI CTCAG 3 cut(s) 400, 466, 559
BspLI GGNNCC 3 cut(s) 12, 155, 387
BspTNI GGTCTC 1 cut(s) 132
BsrFI RCCGGY 1 cut(s) 169
BsrI ACTGG 2 cut(s) 12, 225
BssAI RCCGGY 1 cut(s) 169
BssMI GATC 1 cut(s) 415
Bst6I CTCTTC 1 cut(s) 447
BstAPI GCANNNNNTGC 1 cut(s) 263
BstC8I GCNNGC 2 cut(s) 29, 81
BstDEI CTNAG 3 cut(s) 408, 474, 567
BstF5I GGATG 3 cut(s) 81, 343, 370
BstKTI GATC 1 cut(s) 418
BstMAI GTCTC 1 cut(s) 132
BstMBI GATC 1 cut(s) 415
BstMWI GCNNNNNNNGC 4 cut(s) 68, 263, 560, 572
BstSCI CCNGG 1 cut(s) 120
BstSFI CTRYAG 1 cut(s) 267
BstV1I GCAGC 4 cut(s) 85, 107, 316, 575
BstV2I GAAGAC 1 cut(s) 549
BtsCI GGATG 3 cut(s) 81, 343, 370
BtsIMutI CAGTG 1 cut(s) 5
Cac8I GCNNGC 2 cut(s) 29, 81
CaiI CAGNNNCTG 1 cut(s) 101
Cfr10I RCCGGY 1 cut(s) 169
Cfr13I GGNCC 1 cut(s) 154
CseI GACGC 1 cut(s) 58
Csp6I GTAC 3 cut(s) 398, 512, 532
CspAI ACCGGT 1 cut(s) 169
CviAII CATG 5 cut(s) 46, 188, 314, 488, 515
CviQI GTAC 3 cut(s) 398, 512, 532
DdeI CTNAG 3 cut(s) 408, 474, 567
DpnI GATC 1 cut(s) 417
DpnII GATC 1 cut(s) 415
DraI TTTAAA 1 cut(s) 237
Eam1104I CTCTTC 1 cut(s) 447
EarI CTCTTC 1 cut(s) 447
Eco31I GGTCTC 1 cut(s) 132
Eco32I GATATC 1 cut(s) 370
Eco47I GGWCC 1 cut(s) 154
EcoO109I RGGNCCY 1 cut(s) 154
EcoRV GATATC 1 cut(s) 370
FaeI CATG 5 cut(s) 49, 191, 317, 491, 518
FaiI YATR 6 cut(s) 47, 189, 315, 489, 516, 609
FalI AAGNNNNNCTT 2 cut(s) 302, 334
FaqI GGGAC 1 cut(s) 167
FatI CATG 5 cut(s) 45, 187, 313, 487, 514
FbaI TGATCA 1 cut(s) 415
Fnu4HI GCNGC 6 cut(s) 96, 99, 286, 330, 552, 564
FokI GGATG 3 cut(s) 88, 330, 377
Fsp4HI GCNGC 6 cut(s) 96, 99, 286, 330, 552, 564
FspBI CTAG 1 cut(s) 72
GluI GCNGC 6 cut(s) 96, 99, 286, 330, 552, 564
GsaI CCCAGC 1 cut(s) 105
HapII CCGG 3 cut(s) 122, 170, 194
HgaI GACGC 1 cut(s) 58
Hin1II CATG 5 cut(s) 49, 191, 317, 491, 518
HindIII AAGCTT 2 cut(s) 25, 231
HinfI GANTC 2 cut(s) 19, 424
HpaII CCGG 3 cut(s) 122, 170, 194
HphI GGTGA 1 cut(s) 235
Hpy166II GTNNAC 1 cut(s) 522
Hpy188I TCNGA 1 cut(s) 390
Hpy188III TCNNGA 4 cut(s) 137, 362, 502, 581
Hpy8I GTNNAC 1 cut(s) 522
HpyAV CCTTC 1 cut(s) 357
HpyCH4IV ACGT 1 cut(s) 524
HpyCH4V TGCA 6 cut(s) 83, 95, 191, 215, 257, 467
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 263, 560, 572
HpyF3I CTNAG 3 cut(s) 408, 474, 567
HpySE526I ACGT 1 cut(s) 524
Hsp92II CATG 5 cut(s) 49, 191, 317, 491, 518
Ksp22I TGATCA 1 cut(s) 415
Kzo9I GATC 1 cut(s) 415
LmnI GCTCC 5 cut(s) 16, 67, 123, 404, 604
LpnPI CCDG 8 cut(s) 29, 87, 135, 183, 206, 207, 347, 486
Lsp1109I GCAGC 4 cut(s) 85, 107, 316, 575
LweI GCATC 2 cut(s) 66, 202
MaeI CTAG 1 cut(s) 72
MaeII ACGT 1 cut(s) 524
MaeIII GTNAC 2 cut(s) 3, 355
MalI GATC 1 cut(s) 417
MboI GATC 1 cut(s) 415
MboII GAAGA 6 cut(s) 28, 121, 221, 464, 505, 554
MhlI GDGCHC 1 cut(s) 120
MluCI AATT 2 cut(s) 90, 446
MlyI GAGTC 2 cut(s) 13, 418
MnlI CCTC 6 cut(s) 172, 448, 522, 541, 562, 565
MroXI GAANNNNTTC 1 cut(s) 497
MseI TTAA 5 cut(s) 147, 236, 381, 461, 480
MslI CAYNNNNRTG 1 cut(s) 113
MspA1I CMGCKG 3 cut(s) 98, 421, 566
MspI CCGG 3 cut(s) 122, 170, 194
MspR9I CCNGG 1 cut(s) 122
MwoI GCNNNNNNNGC 4 cut(s) 68, 263, 560, 572
NciI CCSGG 1 cut(s) 122
NdeII GATC 1 cut(s) 415
NlaIII CATG 5 cut(s) 49, 191, 317, 491, 518
NlaIV GGNNCC 3 cut(s) 12, 155, 387
NmuCI GTSAC 2 cut(s) 3, 355
PcsI WCGNNNNNNNCGW 1 cut(s) 543
PdmI GAANNNNTTC 1 cut(s) 497
PfoI TCCNGGA 1 cut(s) 120
PinAI ACCGGT 1 cut(s) 169
PkrI GCNGC 6 cut(s) 97, 100, 287, 331, 553, 565
PleI GAGTC 2 cut(s) 13, 418
PpsI GAGTC 2 cut(s) 13, 418
PpuMI RGGWCCY 1 cut(s) 154
Psp5II RGGWCCY 1 cut(s) 154
PspFI CCCAGC 1 cut(s) 101
PspN4I GGNNCC 3 cut(s) 12, 155, 387
PspPI GGNCC 1 cut(s) 154
PspPPI RGGWCCY 1 cut(s) 154
PstNI CAGNNNCTG 1 cut(s) 101
PvuII CAGCTG 3 cut(s) 98, 421, 566
RsaI GTAC 3 cut(s) 399, 513, 533
RsaNI GTAC 3 cut(s) 398, 512, 532
RseI CAYNNNNRTG 1 cut(s) 113
SaqAI TTAA 5 cut(s) 147, 236, 381, 461, 480
SatI GCNGC 6 cut(s) 96, 99, 286, 330, 552, 564
Sau3AI GATC 1 cut(s) 415
Sau96I GGNCC 1 cut(s) 154
SchI GAGTC 2 cut(s) 13, 418
ScrFI CCNGG 1 cut(s) 122
SduI GDGCHC 1 cut(s) 120
SfaNI GCATC 2 cut(s) 66, 202
SfcI CTRYAG 1 cut(s) 267
SinI GGWCC 1 cut(s) 154
SmiMI CAYNNNNRTG 1 cut(s) 113
Sse9I AATT 2 cut(s) 90, 446
SsiI CCGC 3 cut(s) 285, 377, 551
SspMI CTAG 1 cut(s) 72
StyD4I CCNGG 1 cut(s) 120
TaiI ACGT 1 cut(s) 527
TaqI TCGA 2 cut(s) 136, 207
TasI AATT 2 cut(s) 90, 446
TatI WGTACW 1 cut(s) 397
TauI GCSGC 2 cut(s) 288, 554
Tru1I TTAA 5 cut(s) 147, 236, 381, 461, 480
Tru9I TTAA 5 cut(s) 147, 236, 381, 461, 480
TscAI CASTG 1 cut(s) 12
TseFI GTSAC 2 cut(s) 3, 355
TseI GCWGC 4 cut(s) 95, 98, 329, 563
Tsp45I GTSAC 2 cut(s) 3, 355
TspDTI ATGAA 3 cut(s) 97, 339, 555
TspRI CASTG 1 cut(s) 12
VpaK11BI GGWCC 1 cut(s) 154
XcmI CCANNNNNNNNNTGG 1 cut(s) 52
XmnI GAANNNNTTC 1 cut(s) 497
XspI CTAG 1 cut(s) 72
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.