Rh6DG333300

1,4-beta-D-glucanase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000177
Physical Location & Seq
Reverse (-)
53817896 .. 53821381
3486 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG333300.1

Sequence Viewer

Length: 606 bp
ATGTTCACCATCGTATTACATGTACTCTTTGTGACTTTGTGTCTGCAACTGCAACAAGGTGTGTCTGGTCCTCAATGCTTGGAGCATCCACCGAACCTTAACGGAACCTCAGGAGCTGGATCAGTCATTAAAGTTGGAGGCTTGAAGGCCTATGTTACTGGTCCTTCTCATTCCAAGCTTGCCATAATCCTCGCTTCTGACGTCTACGGATTTGAAGCACCAAACTTAAGGAAAATTGCAGATAAAGTTGCTAGCACTGCTGGATTCCATGTTGTGGTTCCAGACTTCTTTTATGGAGAGCCATACAATCCTAACAACACTCAGAGGAATATGACAGTCTGGCTAAAGGACCATGGAACAGACAAAGGTTTTGAAGATGCTAAACGTTTAATTGCTGCTCTAAAGAACAAAGGGGTATCCAAGATAGGAGCTGCAGGGTTCTGTTGGGGCGGCAAAGTAGTTGCGGAGCTATCAAAGGGTTATTACACTAAGGCTGCAGTTTTGTTACACCCTTCCCGTGTCACTCTTGATGATATTAAAGGTACGGAATCCCTTGCACAATTCACAGTTGAAAAACCACTACTTTCAAAAATATTGATGTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00361 ko00364 ko00623 ko01100 ko01110 ko01120 ko01130 map00361 map00364 map00623 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
DLH

Protein Analysis

201

Amino Acids

21.59

Weight (kDa)

9.2

Isoelectric Point (pI)

19.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DLH PF01738 49 - 178 2.1e-20 Dienelactone hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000177)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23570 AT3G23570 AT3G23570 AT3G23600 AT3G23600
fragaria_vesca FvH4_2g23960 FvH4_2g23960 FvH4_2g23960 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14330 FvH4_6g28031 FvH4_6g29270
malus_domestica MD13G1181200.v1.1 MD13G1181300.v1.1 MD13G1181400.v1.1 MD13G1181500.v1.1 MD13G1181600.v1.1 MD13G1181700.v1.1 MD13G1181800.v1.1 MD13G1181900.v1.1 MD15G1403300.v1.1 MD15G1403400.v1.1 MD16G1181900.v1.1 MD16G1182000.v1.1 MD16G1182200.v1.1 MD16G1182300.v1.1 MD16G1182400.v1.1
prunus_persica Prupe.1G149400_v2.0.a1 Prupe.1G149600_v2.0.a1 Prupe.1G149700_v2.0.a1 Prupe.1G149800_v2.0.a1 Prupe.1G549400_v2.0.a1 Prupe.8G245600_v2.0.a1
pyrus_communis pycom13g15640 pycom13g15650 pycom13g15670 pycom13g15700 pycom15g36070 pycom16g15270 pycom16g15300 pycom16g15310
rosa_chinensis RchiOBHm_Chr1g0334461 RchiOBHm_Chr1g0334471 RchiOBHm_Chr2g0131731 RchiOBHm_Chr4g0417881 RchiOBHm_Chr4g0418561 RchiOBHm_Chr4g0418571 RchiOBHm_Chr4g0418581 RchiOBHm_Chr4g0418591 RchiOBHm_Chr4g0418601 RchiOBHm_Chr4g0418621 RchiOBHm_Chr4g0418651 RchiOBHm_Chr4g0418661 RchiOBHm_Chr4g0418671 RchiOBHm_Chr4g0418681 RchiOBHm_Chr4g0418691 RchiOBHm_Chr4g0418701 RchiOBHm_Chr4g0421371 RchiOBHm_Chr6g0291601 RchiOBHm_Chr6g0291611 RchiOBHm_Chr6g0291621 RchiOBHm_Chr6g0291631 RchiOBHm_Chr7g0229821
rosa_laevigata RLG00000007843 RLG00000007844 RLG00000007846 RLG00000007847 RLG00000007849 RLG00000012101 RLG00000012104 RLG00000029451
rosa_multiflora Rmu_sc0000363.1_g000035 Rmu_sc0000935.1_g000003 Rmu_sc0000935.1_g000010 Rmu_sc0002226.1_g000008 Rmu_sc0002226.1_g000014 Rmu_sc0002589.1_g000031 Rmu_sc0003443.1_g000009 Rmu_sc0006431.1_g000001 Rmu_sc0006431.1_g000006 Rmu_sc0006431.1_g000023 Rmu_sc0006705.1_g000014 Rmu_sc0006705.1_g000015 Rmu_sc0006705.1_g000016 Rmu_sc0006705.1_g000017 Rmu_sc0006705.1_g000018 Rmu_sc0014797.1_g000001 Rmu_sc0041885.1_g000001
rosa_roxburghii Rroxscaffold_4G00316820 Rroxscaffold_5G00361830 Rroxscaffold_5G00361840 Rroxscaffold_5G00361850 Rroxscaffold_5G00361860 Rroxscaffold_5G00361880 Rroxscaffold_5G00361890 Rroxscaffold_5G00361900 Rroxscaffold_5G00361910 Rroxscaffold_5G00361920 Rroxscaffold_5G00361930 Rroxscaffold_7G00175910 Rroxscaffold_7G00175930 Rroxscaffold_7G00175940
rosa_rugosa Rorug02G0299300 Rorug04G0154500 Rorug04G0154600 Rorug04G0154700 Rorug06G0220500
rosa_samantha Rh1AG006300 Rh1AG139200 Rh1AG139300 Rh1BG105600 Rh1CG131200 Rh1CG131300 Rh1DG143200 Rh2AG210000 Rh2CG337500 Rh2DG216200 Rh2DG376200 Rh4AG215900 Rh4AG216000 Rh4AG216100 Rh4AG216200 Rh4AG216300 Rh4AG216400 Rh4BG214300 Rh4BG214400 Rh4BG214500 Rh4BG214600 Rh4BG214700 Rh4BG214800 Rh4CG212300 Rh4CG227500 Rh4CG227700 Rh4CG227900 Rh4CG228000 Rh4CG228100 Rh4DG214200 Rh4DG215000 Rh4DG215200 Rh5AG059400 Rh6AG332900 Rh6AG333000 Rh6AG333100 Rh6BG339700 Rh6BG339900 Rh6CG346100 Rh6CG346200 Rh6DG333300 Rh6DG333400 Rh7AG435400 Rh7CG454500
rosa_wichuraiana Rw1G011480 Rw2G028580 Rw4G018270 Rw4G018280 Rw4G018290 Rw4G018360 Rw4G018390 Rw4G018400 Rw4G018410 Rw4G018420 Rw6G028910 Rw6G029000 Rw6G029010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 204
AccB7I CCANNNNNTGG 1 cut(s) 274
AccI GTMKAC 1 cut(s) 204
AciI CCGC 2 cut(s) 450, 464
AclI AACGTT 1 cut(s) 385
AclWI GGATC 1 cut(s) 127
AcyI GRCGYC 1 cut(s) 201
AfaI GTAC 2 cut(s) 24, 544
AfiI CCNNNNNNNGG 1 cut(s) 274
AflII CTTAAG 1 cut(s) 226
AflIII ACRYGT 1 cut(s) 19
AgsI TTSAA 5 cut(s) 145, 215, 374, 572, 588
AhdI GACNNNNNGTC 1 cut(s) 39
AluBI AGCT 4 cut(s) 116, 178, 431, 469
AluI AGCT 4 cut(s) 116, 178, 431, 469
AlwI GGATC 1 cut(s) 127
AlwNI CAGNNNCTG 1 cut(s) 116
AoxI GGCC 1 cut(s) 147
ApeKI GCWGC 3 cut(s) 395, 431, 494
ArsI GACNNNNNNTTYG 2 cut(s) 353, 385
AspS9I GGNCC 3 cut(s) 68, 161, 349
AsuNHI GCTAGC 1 cut(s) 251
AvaII GGWCC 3 cut(s) 68, 161, 349
AxyI CCTNAGG 1 cut(s) 109
BbvI GCAGC 3 cut(s) 382, 418, 481
BccI CCATC 1 cut(s) 17
BciVI GTATCC 1 cut(s) 427
BfaI CTAG 1 cut(s) 252
BfmI CTRYAG 2 cut(s) 432, 495
BfrI CTTAAG 1 cut(s) 226
BfuI GTATCC 1 cut(s) 427
BisI GCNGC 4 cut(s) 396, 432, 451, 495
BlsI GCNGC 4 cut(s) 397, 433, 452, 496
Bme18I GGWCC 3 cut(s) 68, 161, 349
BmeRI GACNNNNNGTC 1 cut(s) 39
BmgT120I GGNCC 3 cut(s) 68, 161, 349
BmiI GGNNCC 2 cut(s) 106, 279
BmsI GCATC 2 cut(s) 94, 367
BmtI GCTAGC 1 cut(s) 255
BsaHI GRCGYC 1 cut(s) 201
BsaJI CCNNGG 1 cut(s) 352
Bsc4I CCNNNNNNNGG 1 cut(s) 274
Bse1I ACTGG 1 cut(s) 163
Bse21I CCTNAGG 1 cut(s) 109
BseDI CCNNGG 1 cut(s) 352
BseGI GGATG 1 cut(s) 85
BseLI CCNNNNNNNGG 1 cut(s) 274
BseMII CTCAG 2 cut(s) 123, 335
BseNI ACTGG 1 cut(s) 163
BseXI GCAGC 3 cut(s) 382, 418, 481
BshFI GGCC 1 cut(s) 149
BslI CCNNNNNNNGG 1 cut(s) 274
BsnI GGCC 1 cut(s) 149
Bsp143I GATC 1 cut(s) 119
Bsp19I CCATGG 1 cut(s) 352
BspACI CCGC 2 cut(s) 450, 464
BspANI GGCC 1 cut(s) 149
BspCNI CTCAG 2 cut(s) 122, 334
BspLI GGNNCC 2 cut(s) 106, 279
BspMAI CTGCAG 2 cut(s) 436, 499
BspOI GCTAGC 1 cut(s) 255
BspPI GGATC 1 cut(s) 127
BspTI CTTAAG 1 cut(s) 226
BsrI ACTGG 1 cut(s) 163
BssECI CCNNGG 1 cut(s) 352
BssMI GATC 1 cut(s) 119
BssNI GRCGYC 1 cut(s) 201
BssT1I CCWWGG 1 cut(s) 352
Bst4CI ACNGT 2 cut(s) 337, 568
BstACI GRCGYC 1 cut(s) 201
BstAFI CTTAAG 1 cut(s) 226
BstC8I GCNNGC 2 cut(s) 180, 253
BstDEI CTNAG 3 cut(s) 109, 321, 489
BstDSI CCRYGG 1 cut(s) 352
BstF5I GGATG 1 cut(s) 85
BstKTI GATC 1 cut(s) 122
BstMBI GATC 1 cut(s) 119
BstMWI GCNNNNNNNGC 1 cut(s) 257
BstNSI RCATGY 1 cut(s) 23
BstSFI CTRYAG 2 cut(s) 432, 495
BstV1I GCAGC 3 cut(s) 382, 418, 481
Bsu36I CCTNAGG 1 cut(s) 109
BsuI GTATCC 1 cut(s) 427
BsuRI GGCC 1 cut(s) 149
BtgI CCRYGG 1 cut(s) 352
BtsCI GGATG 1 cut(s) 85
BtsI GCAGTG 1 cut(s) 255
BtsIMutI CAGTG 1 cut(s) 255
Cac8I GCNNGC 2 cut(s) 180, 253
CaiI CAGNNNCTG 1 cut(s) 116
Cfr13I GGNCC 3 cut(s) 68, 161, 349
Csp6I GTAC 2 cut(s) 23, 543
CviAII CATG 3 cut(s) 20, 269, 353
CviJI RGCY 9 cut(s) 116, 141, 149, 178, 301, 343, 431, 469, 494
CviKI_1 RGCY 9 cut(s) 116, 141, 149, 178, 301, 343, 431, 469, 494
CviQI GTAC 2 cut(s) 23, 543
DdeI CTNAG 3 cut(s) 109, 321, 489
DpnI GATC 1 cut(s) 121
DpnII GATC 1 cut(s) 119
DriI GACNNNNNGTC 1 cut(s) 39
Eam1105I GACNNNNNGTC 1 cut(s) 39
Eco130I CCWWGG 1 cut(s) 352
Eco147I AGGCCT 1 cut(s) 149
Eco47I GGWCC 3 cut(s) 68, 161, 349
Eco81I CCTNAGG 1 cut(s) 109
EcoT14I CCWWGG 1 cut(s) 352
ErhI CCWWGG 1 cut(s) 352
FaeI CATG 3 cut(s) 23, 272, 356
FaiI YATR 8 cut(s) 21, 153, 185, 270, 294, 304, 332, 354
FatI CATG 3 cut(s) 19, 268, 352
FblI GTMKAC 1 cut(s) 204
Fnu4HI GCNGC 4 cut(s) 396, 432, 451, 495
FokI GGATG 1 cut(s) 72
Fsp4HI GCNGC 4 cut(s) 396, 432, 451, 495
FspBI CTAG 1 cut(s) 252
GluI GCNGC 4 cut(s) 396, 432, 451, 495
HaeIII GGCC 1 cut(s) 149
Hin1I GRCGYC 1 cut(s) 201
Hin1II CATG 3 cut(s) 23, 272, 356
HindIII AAGCTT 1 cut(s) 176
HinfI GANTC 2 cut(s) 264, 548
Hpy166II GTNNAC 2 cut(s) 6, 205
Hpy188I TCNGA 2 cut(s) 199, 324
Hpy188III TCNNGA 4 cut(s) 111, 281, 527, 603
Hpy8I GTNNAC 2 cut(s) 6, 205
HpyAV CCTTC 3 cut(s) 139, 174, 522
HpyCH4III ACNGT 2 cut(s) 337, 568
HpyCH4IV ACGT 2 cut(s) 201, 385
HpyCH4V TGCA 6 cut(s) 46, 52, 239, 434, 497, 557
HpyF10VI GCNNNNNNNGC 1 cut(s) 257
HpyF3I CTNAG 3 cut(s) 109, 321, 489
HpySE526I ACGT 2 cut(s) 201, 385
Hsp92I GRCGYC 1 cut(s) 201
Hsp92II CATG 3 cut(s) 23, 272, 356
Kzo9I GATC 1 cut(s) 119
LmnI GCTCC 4 cut(s) 82, 113, 428, 466
LpnPI CCDG 8 cut(s) 51, 96, 102, 144, 246, 294, 325, 420
Lsp1109I GCAGC 3 cut(s) 382, 418, 481
LweI GCATC 2 cut(s) 94, 367
MaeI CTAG 1 cut(s) 252
MaeII ACGT 2 cut(s) 201, 385
MaeIII GTNAC 4 cut(s) 31, 154, 504, 520
MalI GATC 1 cut(s) 121
MboI GATC 1 cut(s) 119
MboII GAAGA 1 cut(s) 386
MluCI AATT 3 cut(s) 234, 390, 560
MmeI TCCRAC 1 cut(s) 115
MnlI CCTC 5 cut(s) 81, 118, 131, 200, 318
MseI TTAA 5 cut(s) 99, 129, 227, 389, 537
MspCI CTTAAG 1 cut(s) 226
MwoI GCNNNNNNNGC 1 cut(s) 257
NcoI CCATGG 1 cut(s) 352
NdeII GATC 1 cut(s) 119
NheI GCTAGC 1 cut(s) 251
NlaIII CATG 3 cut(s) 23, 272, 356
NlaIV GGNNCC 2 cut(s) 106, 279
NmuCI GTSAC 2 cut(s) 31, 520
NspI RCATGY 1 cut(s) 23
PceI AGGCCT 1 cut(s) 149
PciI ACATGT 1 cut(s) 19
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PfeI GAWTC 2 cut(s) 264, 548
PflMI CCANNNNNTGG 1 cut(s) 274
PkrI GCNGC 4 cut(s) 397, 433, 452, 496
PscI ACATGT 1 cut(s) 19
Psp1406I AACGTT 1 cut(s) 385
PspN4I GGNNCC 2 cut(s) 106, 279
PspPI GGNCC 3 cut(s) 68, 161, 349
PstI CTGCAG 2 cut(s) 436, 499
PstNI CAGNNNCTG 1 cut(s) 116
RsaI GTAC 2 cut(s) 24, 544
RsaNI GTAC 2 cut(s) 23, 543
SaqAI TTAA 5 cut(s) 99, 129, 227, 389, 537
SatI GCNGC 4 cut(s) 396, 432, 451, 495
Sau3AI GATC 1 cut(s) 119
Sau96I GGNCC 3 cut(s) 68, 161, 349
SfaNI GCATC 2 cut(s) 94, 367
SfcI CTRYAG 2 cut(s) 432, 495
SinI GGWCC 3 cut(s) 68, 161, 349
SmlI CTYRAG 1 cut(s) 226
SmoI CTYRAG 1 cut(s) 226
Sse9I AATT 3 cut(s) 234, 390, 560
SseBI AGGCCT 1 cut(s) 149
SsiI CCGC 2 cut(s) 450, 464
SspI AATATT 1 cut(s) 594
SspMI CTAG 1 cut(s) 252
StuI AGGCCT 1 cut(s) 149
StyI CCWWGG 1 cut(s) 352
TaaI ACNGT 2 cut(s) 337, 568
TaiI ACGT 2 cut(s) 204, 388
TasI AATT 3 cut(s) 234, 390, 560
TatI WGTACW 1 cut(s) 22
TauI GCSGC 1 cut(s) 453
TfiI GAWTC 2 cut(s) 264, 548
Tru1I TTAA 5 cut(s) 99, 129, 227, 389, 537
Tru9I TTAA 5 cut(s) 99, 129, 227, 389, 537
TscAI CASTG 1 cut(s) 262
TseFI GTSAC 2 cut(s) 31, 520
TseI GCWGC 3 cut(s) 395, 431, 494
Tsp45I GTSAC 2 cut(s) 31, 520
TspGWI ACGGA 3 cut(s) 117, 222, 560
TspRI CASTG 1 cut(s) 262
Van91I CCANNNNNTGG 1 cut(s) 274
Vha464I CTTAAG 1 cut(s) 226
VpaK11BI GGWCC 3 cut(s) 68, 161, 349
XceI RCATGY 1 cut(s) 23
XmiI GTMKAC 1 cut(s) 204
XspI CTAG 1 cut(s) 252
ZraI GACGTC 1 cut(s) 202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.