MD13G1181200.v1.1

1,4-beta-D-glucanase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0000177
Physical Location & Seq
Reverse (-)
15095770 .. 15097839
2070 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1181200.v1.1.491

Sequence Viewer

Length: 570 bp
ATGCGACCTGCCCTTCTCTTTCAAAGGAAGCTTGCAGACAAAATTGCAGCTGCTGGGTTCATTGTTGTGCTCCCAGACTTCTTCAACGGAGACCCTTTTAATGGGGACCATGCCTCTATACCCGTTTGGAGAAAAACTCATGCACCGGAAAAAACAATCGAAGATGCAAAACTGGTCATTGAAGCTTTAAGAAGTAAAGGTGTTTCTGCAATTGGTGCTGCAGGCTTCTGTTGGGGCACCAAGGGTGTCATTGATCTTGCGAAGCATGACTTTATCCAAGCCGCTGTTCTGTGTCATCCTTCATCTGTCACTCTGGATGATATCAAGGCTGTTAAGGTTCCGATTTCTGTACTTGGAGCTGAGATTGATCAGATGTATCCACCCGAAGTCATCAAACAATTTGAAGAGGTTTTAACTGCAAAACCTGAGATTAAAAGCCATGTGAAGATATTCCCGAAGGTGGCACACGGGTGGACGTTGAGGTACGACGATGAAGACGAGGCGGCTGTCAAGGCGGCTGGGGAGGCTCATCAAGATTTGTTGGGGTGGTTCTTGGAGCATGTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
ko00361 ko00364 ko00623 ko01100 ko01110 ko01120 ko01130 map00361 map00364 map00623 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
DLH

Protein Analysis

190

Amino Acids

20.76

Weight (kDa)

6.05

Isoelectric Point (pI)

31.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DLH PF01738 9 - 186 6.5e-25 Dienelactone hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000177)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23570 AT3G23570 AT3G23570 AT3G23600 AT3G23600
fragaria_vesca FvH4_2g23960 FvH4_2g23960 FvH4_2g23960 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14330 FvH4_6g28031 FvH4_6g29270
malus_domestica MD13G1181200.v1.1 MD13G1181300.v1.1 MD13G1181400.v1.1 MD13G1181500.v1.1 MD13G1181600.v1.1 MD13G1181700.v1.1 MD13G1181800.v1.1 MD13G1181900.v1.1 MD15G1403300.v1.1 MD15G1403400.v1.1 MD16G1181900.v1.1 MD16G1182000.v1.1 MD16G1182200.v1.1 MD16G1182300.v1.1 MD16G1182400.v1.1
prunus_persica Prupe.1G149400_v2.0.a1 Prupe.1G149600_v2.0.a1 Prupe.1G149700_v2.0.a1 Prupe.1G149800_v2.0.a1 Prupe.1G549400_v2.0.a1 Prupe.8G245600_v2.0.a1
pyrus_communis pycom13g15640 pycom13g15650 pycom13g15670 pycom13g15700 pycom15g36070 pycom16g15270 pycom16g15300 pycom16g15310
rosa_chinensis RchiOBHm_Chr1g0334461 RchiOBHm_Chr1g0334471 RchiOBHm_Chr2g0131731 RchiOBHm_Chr4g0417881 RchiOBHm_Chr4g0418561 RchiOBHm_Chr4g0418571 RchiOBHm_Chr4g0418581 RchiOBHm_Chr4g0418591 RchiOBHm_Chr4g0418601 RchiOBHm_Chr4g0418621 RchiOBHm_Chr4g0418651 RchiOBHm_Chr4g0418661 RchiOBHm_Chr4g0418671 RchiOBHm_Chr4g0418681 RchiOBHm_Chr4g0418691 RchiOBHm_Chr4g0418701 RchiOBHm_Chr4g0421371 RchiOBHm_Chr6g0291601 RchiOBHm_Chr6g0291611 RchiOBHm_Chr6g0291621 RchiOBHm_Chr6g0291631 RchiOBHm_Chr7g0229821
rosa_laevigata RLG00000007843 RLG00000007844 RLG00000007846 RLG00000007847 RLG00000007849 RLG00000012101 RLG00000012104 RLG00000029451
rosa_multiflora Rmu_sc0000363.1_g000035 Rmu_sc0000935.1_g000003 Rmu_sc0000935.1_g000010 Rmu_sc0002226.1_g000008 Rmu_sc0002226.1_g000014 Rmu_sc0002589.1_g000031 Rmu_sc0003443.1_g000009 Rmu_sc0006431.1_g000001 Rmu_sc0006431.1_g000006 Rmu_sc0006431.1_g000023 Rmu_sc0006705.1_g000014 Rmu_sc0006705.1_g000015 Rmu_sc0006705.1_g000016 Rmu_sc0006705.1_g000017 Rmu_sc0006705.1_g000018 Rmu_sc0014797.1_g000001 Rmu_sc0041885.1_g000001
rosa_roxburghii Rroxscaffold_4G00316820 Rroxscaffold_5G00361830 Rroxscaffold_5G00361840 Rroxscaffold_5G00361850 Rroxscaffold_5G00361860 Rroxscaffold_5G00361880 Rroxscaffold_5G00361890 Rroxscaffold_5G00361900 Rroxscaffold_5G00361910 Rroxscaffold_5G00361920 Rroxscaffold_5G00361930 Rroxscaffold_7G00175910 Rroxscaffold_7G00175930 Rroxscaffold_7G00175940
rosa_rugosa Rorug02G0299300 Rorug04G0154500 Rorug04G0154600 Rorug04G0154700 Rorug06G0220500
rosa_samantha Rh1AG006300 Rh1AG139200 Rh1AG139300 Rh1BG105600 Rh1CG131200 Rh1CG131300 Rh1DG143200 Rh2AG210000 Rh2CG337500 Rh2DG216200 Rh2DG376200 Rh4AG215900 Rh4AG216000 Rh4AG216100 Rh4AG216200 Rh4AG216300 Rh4AG216400 Rh4BG214300 Rh4BG214400 Rh4BG214500 Rh4BG214600 Rh4BG214700 Rh4BG214800 Rh4CG212300 Rh4CG227500 Rh4CG227700 Rh4CG227900 Rh4CG228000 Rh4CG228100 Rh4DG214200 Rh4DG215000 Rh4DG215200 Rh5AG059400 Rh6AG332900 Rh6AG333000 Rh6AG333100 Rh6BG339700 Rh6BG339900 Rh6CG346100 Rh6CG346200 Rh6DG333300 Rh6DG333400 Rh7AG435400 Rh7CG454500
rosa_wichuraiana Rw1G011480 Rw2G028580 Rw4G018270 Rw4G018280 Rw4G018290 Rw4G018360 Rw4G018390 Rw4G018400 Rw4G018410 Rw4G018420 Rw6G028910 Rw6G029000 Rw6G029010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 16
AccB1I GGYRCC 1 cut(s) 236
AciI CCGC 3 cut(s) 282, 503, 515
AfaI GTAC 2 cut(s) 351, 485
AfiI CCNNNNNNNGG 2 cut(s) 101, 460
AgsI TTSAA 4 cut(s) 23, 85, 182, 404
AjuI GAANNNNNNNTTGG 2 cut(s) 270, 302
AleI CACNNNNGTG 1 cut(s) 469
AluBI AGCT 4 cut(s) 31, 50, 185, 359
AluI AGCT 4 cut(s) 31, 50, 185, 359
Alw21I GWGCWC 1 cut(s) 72
Alw26I GTCTC 1 cut(s) 84
AlwNI CAGNNNCTG 1 cut(s) 53
ApeKI GCWGC 3 cut(s) 47, 50, 218
Asp700I GAANNNNTTC 1 cut(s) 449
AspS9I GGNCC 1 cut(s) 106
AvaII GGWCC 1 cut(s) 106
BaeGI GKGCMC 1 cut(s) 239
BanI GGYRCC 1 cut(s) 236
BbsI GAAGAC 1 cut(s) 501
Bbv12I GWGCWC 1 cut(s) 72
BbvI GCAGC 3 cut(s) 37, 59, 205
BciVI GTATCC 1 cut(s) 387
BclI TGATCA 1 cut(s) 367
BcoDI GTCTC 1 cut(s) 84
BfmI CTRYAG 1 cut(s) 219
BfuAI ACCTGC 1 cut(s) 16
BfuI GTATCC 1 cut(s) 387
BisI GCNGC 6 cut(s) 48, 51, 219, 282, 504, 516
BlsI GCNGC 6 cut(s) 49, 52, 220, 283, 505, 517
Bme18I GGWCC 1 cut(s) 106
BmgT120I GGNCC 1 cut(s) 106
BmiI GGNNCC 3 cut(s) 107, 238, 339
BmsI GCATC 1 cut(s) 154
BpiI GAAGAC 1 cut(s) 501
BplI GAGNNNNNCTC 2 cut(s) 121, 153
BsaI GGTCTC 1 cut(s) 84
BsaJI CCNNGG 1 cut(s) 240
BsaWI WCCGGW 1 cut(s) 145
Bsc4I CCNNNNNNNGG 2 cut(s) 101, 460
Bse1I ACTGG 1 cut(s) 177
BseDI CCNNGG 1 cut(s) 240
BseGI GGATG 2 cut(s) 295, 322
BseLI CCNNNNNNNGG 2 cut(s) 101, 460
BseMII CTCAG 2 cut(s) 351, 417
BseNI ACTGG 1 cut(s) 177
BseSI GKGCMC 1 cut(s) 239
BseXI GCAGC 3 cut(s) 37, 59, 205
BseYI CCCAGC 2 cut(s) 53, 518
BshNI GGYRCC 1 cut(s) 236
BsiHKAI GWGCWC 1 cut(s) 72
BsiSI CCGG 1 cut(s) 146
BslFI GGGAC 1 cut(s) 119
BslI CCNNNNNNNGG 2 cut(s) 101, 460
BsmAI GTCTC 1 cut(s) 84
BsmFI GGGAC 1 cut(s) 119
Bso31I GGTCTC 1 cut(s) 84
Bsp1286I GDGCHC 2 cut(s) 72, 239
Bsp143I GATC 2 cut(s) 253, 367
BspACI CCGC 3 cut(s) 282, 503, 515
BspCNI CTCAG 2 cut(s) 352, 418
BspLI GGNNCC 3 cut(s) 107, 238, 339
BspMAI CTGCAG 1 cut(s) 223
BspMI ACCTGC 1 cut(s) 16
BspT107I GGYRCC 1 cut(s) 236
BspTNI GGTCTC 1 cut(s) 84
BsrI ACTGG 1 cut(s) 177
BssECI CCNNGG 1 cut(s) 240
BssMI GATC 2 cut(s) 253, 367
BssT1I CCWWGG 1 cut(s) 240
Bst6I CTCTTC 1 cut(s) 399
BstAPI GCANNNNNTGC 1 cut(s) 215
BstC8I GCNNGC 2 cut(s) 33, 223
BstDEI CTNAG 2 cut(s) 360, 426
BstF5I GGATG 2 cut(s) 295, 322
BstKTI GATC 2 cut(s) 256, 370
BstMAI GTCTC 1 cut(s) 84
BstMBI GATC 2 cut(s) 253, 367
BstMWI GCNNNNNNNGC 3 cut(s) 215, 512, 524
BstNSI RCATGY 1 cut(s) 563
BstSFI CTRYAG 1 cut(s) 219
BstSLI GKGCMC 1 cut(s) 239
BstV1I GCAGC 3 cut(s) 37, 59, 205
BstV2I GAAGAC 1 cut(s) 501
BsuI GTATCC 1 cut(s) 387
BtsCI GGATG 2 cut(s) 295, 322
BveI ACCTGC 1 cut(s) 16
Cac8I GCNNGC 2 cut(s) 33, 223
CaiI CAGNNNCTG 1 cut(s) 53
Cfr13I GGNCC 1 cut(s) 106
Csp6I GTAC 2 cut(s) 350, 484
CviAII CATG 5 cut(s) 110, 140, 266, 440, 560
CviQI GTAC 2 cut(s) 350, 484
DdeI CTNAG 2 cut(s) 360, 426
DpnI GATC 2 cut(s) 255, 369
DpnII GATC 2 cut(s) 253, 367
Eam1104I CTCTTC 1 cut(s) 399
EarI CTCTTC 1 cut(s) 399
Eco130I CCWWGG 1 cut(s) 240
Eco31I GGTCTC 1 cut(s) 84
Eco32I GATATC 1 cut(s) 322
Eco47I GGWCC 1 cut(s) 106
EcoRV GATATC 1 cut(s) 322
EcoT14I CCWWGG 1 cut(s) 240
ErhI CCWWGG 1 cut(s) 240
FaeI CATG 5 cut(s) 113, 143, 269, 443, 563
FaiI YATR 6 cut(s) 111, 119, 141, 267, 441, 561
FalI AAGNNNNNCTT 2 cut(s) 254, 286
FaqI GGGAC 1 cut(s) 119
FatI CATG 5 cut(s) 109, 139, 265, 439, 559
FbaI TGATCA 1 cut(s) 367
Fnu4HI GCNGC 6 cut(s) 48, 51, 219, 282, 504, 516
FokI GGATG 2 cut(s) 282, 329
Fsp4HI GCNGC 6 cut(s) 48, 51, 219, 282, 504, 516
GluI GCNGC 6 cut(s) 48, 51, 219, 282, 504, 516
GsaI CCCAGC 2 cut(s) 57, 522
HapII CCGG 1 cut(s) 146
Hin1II CATG 5 cut(s) 113, 143, 269, 443, 563
HindIII AAGCTT 2 cut(s) 29, 183
HpaII CCGG 1 cut(s) 146
Hpy166II GTNNAC 1 cut(s) 474
Hpy188I TCNGA 2 cut(s) 342, 372
Hpy188III TCNNGA 3 cut(s) 314, 454, 533
Hpy8I GTNNAC 1 cut(s) 474
Hpy99I CGWCG 1 cut(s) 491
HpyAV CCTTC 3 cut(s) 23, 309, 451
HpyCH4IV ACGT 1 cut(s) 476
HpyCH4V TGCA 7 cut(s) 35, 47, 143, 167, 209, 221, 419
HpyF10VI GCNNNNNNNGC 3 cut(s) 215, 512, 524
HpyF3I CTNAG 2 cut(s) 360, 426
HpySE526I ACGT 1 cut(s) 476
Hsp92II CATG 5 cut(s) 113, 143, 269, 443, 563
Ksp22I TGATCA 1 cut(s) 367
Kzo9I GATC 2 cut(s) 253, 367
LmnI GCTCC 3 cut(s) 75, 356, 556
LpnPI CCDG 9 cut(s) 21, 39, 87, 158, 159, 207, 299, 438, 504
Lsp1109I GCAGC 3 cut(s) 37, 59, 205
LweI GCATC 1 cut(s) 154
MaeII ACGT 1 cut(s) 476
MaeIII GTNAC 1 cut(s) 307
MalI GATC 2 cut(s) 255, 369
MboI GATC 2 cut(s) 253, 367
MboII GAAGA 5 cut(s) 73, 173, 416, 457, 506
MfeI CAATTG 1 cut(s) 210
MhlI GDGCHC 2 cut(s) 72, 239
MluCI AATT 3 cut(s) 42, 210, 398
MnlI CCTC 5 cut(s) 124, 400, 474, 493, 517
MroXI GAANNNNTTC 1 cut(s) 449
MseI TTAA 5 cut(s) 99, 188, 333, 413, 432
MslI CAYNNNNRTG 2 cut(s) 65, 469
MspA1I CMGCKG 2 cut(s) 50, 284
MspI CCGG 1 cut(s) 146
MunI CAATTG 1 cut(s) 210
MwoI GCNNNNNNNGC 3 cut(s) 215, 512, 524
NdeII GATC 2 cut(s) 253, 367
NlaIII CATG 5 cut(s) 113, 143, 269, 443, 563
NlaIV GGNNCC 3 cut(s) 107, 238, 339
NmuCI GTSAC 1 cut(s) 307
NspI RCATGY 1 cut(s) 563
OliI CACNNNNGTG 1 cut(s) 469
PcsI WCGNNNNNNNCGW 1 cut(s) 495
PdmI GAANNNNTTC 1 cut(s) 449
PkrI GCNGC 6 cut(s) 49, 52, 220, 283, 505, 517
PspFI CCCAGC 2 cut(s) 53, 518
PspN4I GGNNCC 3 cut(s) 107, 238, 339
PspPI GGNCC 1 cut(s) 106
PstI CTGCAG 1 cut(s) 223
PstNI CAGNNNCTG 1 cut(s) 53
PvuII CAGCTG 1 cut(s) 50
RsaI GTAC 2 cut(s) 351, 485
RsaNI GTAC 2 cut(s) 350, 484
RseI CAYNNNNRTG 2 cut(s) 65, 469
SaqAI TTAA 5 cut(s) 99, 188, 333, 413, 432
SatI GCNGC 6 cut(s) 48, 51, 219, 282, 504, 516
Sau3AI GATC 2 cut(s) 253, 367
Sau96I GGNCC 1 cut(s) 106
SduI GDGCHC 2 cut(s) 72, 239
SfaNI GCATC 1 cut(s) 154
SfcI CTRYAG 1 cut(s) 219
SinI GGWCC 1 cut(s) 106
SmiMI CAYNNNNRTG 2 cut(s) 65, 469
Sse9I AATT 3 cut(s) 42, 210, 398
SsiI CCGC 3 cut(s) 282, 503, 515
StyI CCWWGG 1 cut(s) 240
TaiI ACGT 1 cut(s) 479
TaqI TCGA 1 cut(s) 159
TasI AATT 3 cut(s) 42, 210, 398
TatI WGTACW 1 cut(s) 349
TauI GCSGC 3 cut(s) 284, 506, 518
Tru1I TTAA 5 cut(s) 99, 188, 333, 413, 432
Tru9I TTAA 5 cut(s) 99, 188, 333, 413, 432
TseFI GTSAC 1 cut(s) 307
TseI GCWGC 3 cut(s) 47, 50, 218
Tsp45I GTSAC 1 cut(s) 307
TspDTI ATGAA 3 cut(s) 49, 291, 507
TspGWI ACGGA 1 cut(s) 102
VpaK11BI GGWCC 1 cut(s) 106
XceI RCATGY 1 cut(s) 563
XmnI GAANNNNTTC 1 cut(s) 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.