Rh4CG228100

1,4-beta-D-glucanase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000177
Physical Location & Seq
Forward (+)
46554794 .. 46575789
20996 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG228100.1

Sequence Viewer

Length: 621 bp
ATGTCAGGCCCTGCGTGCTGCTCAAACCCTCCAGCCCTTAACCCAGCAAGTGGTTCCGGCCATGTTGAAAAGCTTGCTGGTCTGGACTCCTATGTCACTGGCTCTCCTGACTCCAAGCTTGCCGTTCTTCTCATTGCTGACATTTTTGGCTATGAAGCTCCACTCTTGAGGAAGCTCGCAGACAAAGTTGCAGCTGCTGGGTTCTTTGTTGTGGTACCTGATTTCTTTTATGGAGATGCTTTTCCTGGTGATGCTGGACCAGTTGGAAACTCTTCTGCTCTACCTATTTGGTTAAAGACACATTTACCGATCAAGGCATCTGAAGCTGCAAAACCATTACTTGAAGCCTTAAAGAGTAAAGGTGTTTCTGCAATTGGGGCTGTAGGCTTCTGTTGGGGAGCCAAGGCTGCGGTTGAACTTGCAAAGTATGATGACCTTCTCCATGCTGCTGTCTTGTGCCATCCTTCATTCGTCACTGTGGACGATTTCAAGGCGGTTAAGGTTCCCATTTCTGTACTTGGAGCTGAGATTGATCCGATTTCGCCACCTGACGTCGTGAAACAATTTGAAGAGGTTTTAACTGCAAGACCTGAGTGTGGTGAATTACTTATGATAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
ko00361 ko00364 ko00623 ko01100 ko01110 ko01120 ko01130 map00361 map00364 map00623 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
DLH

Protein Analysis

206

Amino Acids

21.41

Weight (kDa)

5.02

Isoelectric Point (pI)

33.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DLH PF01738 29 - 196 6.6e-24 Dienelactone hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000177)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23570 AT3G23570 AT3G23570 AT3G23600 AT3G23600
fragaria_vesca FvH4_2g23960 FvH4_2g23960 FvH4_2g23960 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14330 FvH4_6g28031 FvH4_6g29270
malus_domestica MD13G1181200.v1.1 MD13G1181300.v1.1 MD13G1181400.v1.1 MD13G1181500.v1.1 MD13G1181600.v1.1 MD13G1181700.v1.1 MD13G1181800.v1.1 MD13G1181900.v1.1 MD15G1403300.v1.1 MD15G1403400.v1.1 MD16G1181900.v1.1 MD16G1182000.v1.1 MD16G1182200.v1.1 MD16G1182300.v1.1 MD16G1182400.v1.1
prunus_persica Prupe.1G149400_v2.0.a1 Prupe.1G149600_v2.0.a1 Prupe.1G149700_v2.0.a1 Prupe.1G149800_v2.0.a1 Prupe.1G549400_v2.0.a1 Prupe.8G245600_v2.0.a1
pyrus_communis pycom13g15640 pycom13g15650 pycom13g15670 pycom13g15700 pycom15g36070 pycom16g15270 pycom16g15300 pycom16g15310
rosa_chinensis RchiOBHm_Chr1g0334461 RchiOBHm_Chr1g0334471 RchiOBHm_Chr2g0131731 RchiOBHm_Chr4g0417881 RchiOBHm_Chr4g0418561 RchiOBHm_Chr4g0418571 RchiOBHm_Chr4g0418581 RchiOBHm_Chr4g0418591 RchiOBHm_Chr4g0418601 RchiOBHm_Chr4g0418621 RchiOBHm_Chr4g0418651 RchiOBHm_Chr4g0418661 RchiOBHm_Chr4g0418671 RchiOBHm_Chr4g0418681 RchiOBHm_Chr4g0418691 RchiOBHm_Chr4g0418701 RchiOBHm_Chr4g0421371 RchiOBHm_Chr6g0291601 RchiOBHm_Chr6g0291611 RchiOBHm_Chr6g0291621 RchiOBHm_Chr6g0291631 RchiOBHm_Chr7g0229821
rosa_laevigata RLG00000007843 RLG00000007844 RLG00000007846 RLG00000007847 RLG00000007849 RLG00000012101 RLG00000012104 RLG00000029451
rosa_multiflora Rmu_sc0000363.1_g000035 Rmu_sc0000935.1_g000003 Rmu_sc0000935.1_g000010 Rmu_sc0002226.1_g000008 Rmu_sc0002226.1_g000014 Rmu_sc0002589.1_g000031 Rmu_sc0003443.1_g000009 Rmu_sc0006431.1_g000001 Rmu_sc0006431.1_g000006 Rmu_sc0006431.1_g000023 Rmu_sc0006705.1_g000014 Rmu_sc0006705.1_g000015 Rmu_sc0006705.1_g000016 Rmu_sc0006705.1_g000017 Rmu_sc0006705.1_g000018 Rmu_sc0014797.1_g000001 Rmu_sc0041885.1_g000001
rosa_roxburghii Rroxscaffold_4G00316820 Rroxscaffold_5G00361830 Rroxscaffold_5G00361840 Rroxscaffold_5G00361850 Rroxscaffold_5G00361860 Rroxscaffold_5G00361880 Rroxscaffold_5G00361890 Rroxscaffold_5G00361900 Rroxscaffold_5G00361910 Rroxscaffold_5G00361920 Rroxscaffold_5G00361930 Rroxscaffold_7G00175910 Rroxscaffold_7G00175930 Rroxscaffold_7G00175940
rosa_rugosa Rorug02G0299300 Rorug04G0154500 Rorug04G0154600 Rorug04G0154700 Rorug06G0220500
rosa_samantha Rh1AG006300 Rh1AG139200 Rh1AG139300 Rh1BG105600 Rh1CG131200 Rh1CG131300 Rh1DG143200 Rh2AG210000 Rh2CG337500 Rh2DG216200 Rh2DG376200 Rh4AG215900 Rh4AG216000 Rh4AG216100 Rh4AG216200 Rh4AG216300 Rh4AG216400 Rh4BG214300 Rh4BG214400 Rh4BG214500 Rh4BG214600 Rh4BG214700 Rh4BG214800 Rh4CG212300 Rh4CG227500 Rh4CG227700 Rh4CG227900 Rh4CG228000 Rh4CG228100 Rh4DG214200 Rh4DG215000 Rh4DG215200 Rh5AG059400 Rh6AG332900 Rh6AG333000 Rh6AG333100 Rh6BG339700 Rh6BG339900 Rh6CG346100 Rh6CG346200 Rh6DG333300 Rh6DG333400 Rh7AG435400 Rh7CG454500
rosa_wichuraiana Rw1G011480 Rw2G028580 Rw4G018270 Rw4G018280 Rw4G018290 Rw4G018360 Rw4G018390 Rw4G018400 Rw4G018410 Rw4G018420 Rw6G028910 Rw6G029000 Rw6G029010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 92
AatII GACGTC 1 cut(s) 555
Acc65I GGTACC 1 cut(s) 214
AccB1I GGYRCC 1 cut(s) 214
AccB7I CCANNNNNTGG 1 cut(s) 50
AciI CCGC 2 cut(s) 410, 494
AclWI GGATC 1 cut(s) 527
AcoI YGGCCR 1 cut(s) 58
AcuI CTGAAG 1 cut(s) 342
AcyI GRCGYC 1 cut(s) 552
AfaI GTAC 2 cut(s) 216, 516
AfiI CCNNNNNNNGG 2 cut(s) 50, 596
AgsI TTSAA 5 cut(s) 68, 344, 416, 490, 569
AjnI CCWGG 1 cut(s) 244
AluBI AGCT 7 cut(s) 73, 118, 158, 175, 194, 326, 524
AluI AGCT 7 cut(s) 73, 118, 158, 175, 194, 326, 524
AlwI GGATC 1 cut(s) 527
AlwNI CAGNNNCTG 2 cut(s) 11, 197
AoxI GGCC 2 cut(s) 7, 58
ApeKI GCWGC 6 cut(s) 18, 191, 194, 326, 407, 446
Asp700I GAANNNNTTC 1 cut(s) 271
Asp718I GGTACC 1 cut(s) 214
AspS9I GGNCC 2 cut(s) 8, 257
AsuHPI GGTGA 2 cut(s) 260, 611
AvaII GGWCC 1 cut(s) 257
BanI GGYRCC 1 cut(s) 214
BbvI GCAGC 6 cut(s) 5, 181, 203, 313, 394, 433
BccI CCATC 1 cut(s) 468
BceAI ACGGC 1 cut(s) 107
BciT130I CCWGG 1 cut(s) 246
BfmI CTRYAG 1 cut(s) 381
BisI GCNGC 6 cut(s) 19, 192, 195, 327, 408, 447
BlsI GCNGC 6 cut(s) 20, 193, 196, 328, 409, 448
Bme1390I CCNGG 1 cut(s) 246
Bme18I GGWCC 1 cut(s) 257
BmgT120I GGNCC 2 cut(s) 8, 257
BmiI GGNNCC 4 cut(s) 55, 216, 400, 504
BmrFI CCNGG 1 cut(s) 246
BmsI GCATC 3 cut(s) 226, 241, 326
BpmI CTGGAG 1 cut(s) 15
BpuEI CTTGAG 1 cut(s) 187
BsaHI GRCGYC 1 cut(s) 552
BsaJI CCNNGG 1 cut(s) 402
BsaXI ACNNNNNCTCC 2 cut(s) 88, 118
Bsc4I CCNNNNNNNGG 2 cut(s) 50, 596
Bse1I ACTGG 2 cut(s) 103, 260
Bse3DI GCAATG 1 cut(s) 132
BseBI CCWGG 1 cut(s) 246
BseDI CCNNGG 1 cut(s) 402
BseGI GGATG 1 cut(s) 460
BseLI CCNNNNNNNGG 2 cut(s) 50, 596
BseMI GCAATG 1 cut(s) 132
BseMII CTCAG 2 cut(s) 516, 582
BseNI ACTGG 2 cut(s) 103, 260
BseXI GCAGC 6 cut(s) 5, 181, 203, 313, 394, 433
BseYI CCCAGC 2 cut(s) 43, 197
BshFI GGCC 2 cut(s) 9, 60
BshNI GGYRCC 1 cut(s) 214
BsiSI CCGG 1 cut(s) 57
BslI CCNNNNNNNGG 2 cut(s) 50, 596
BsnI GGCC 2 cut(s) 9, 60
Bsp143I GATC 2 cut(s) 309, 532
BspACI CCGC 2 cut(s) 410, 494
BspANI GGCC 2 cut(s) 9, 60
BspCNI CTCAG 2 cut(s) 517, 583
BspLI GGNNCC 4 cut(s) 55, 216, 400, 504
BspPI GGATC 1 cut(s) 527
BspT107I GGYRCC 1 cut(s) 214
BsrDI GCAATG 1 cut(s) 132
BsrI ACTGG 2 cut(s) 103, 260
BssECI CCNNGG 1 cut(s) 402
BssMI GATC 2 cut(s) 309, 532
BssNI GRCGYC 1 cut(s) 552
BssT1I CCWWGG 1 cut(s) 402
Bst2UI CCWGG 1 cut(s) 246
Bst4CI ACNGT 1 cut(s) 478
Bst6I CTCTTC 2 cut(s) 277, 564
BstACI GRCGYC 1 cut(s) 552
BstC8I GCNNGC 4 cut(s) 16, 75, 120, 177
BstDEI CTNAG 2 cut(s) 525, 591
BstF5I GGATG 1 cut(s) 460
BstKTI GATC 2 cut(s) 312, 535
BstMBI GATC 2 cut(s) 309, 532
BstMWI GCNNNNNNNGC 4 cut(s) 15, 323, 377, 407
BstNI CCWGG 1 cut(s) 246
BstSCI CCNGG 1 cut(s) 244
BstSFI CTRYAG 1 cut(s) 381
BstV1I GCAGC 6 cut(s) 5, 181, 203, 313, 394, 433
BsuRI GGCC 2 cut(s) 9, 60
BtsCI GGATG 1 cut(s) 460
BtsIMutI CAGTG 2 cut(s) 96, 474
Cac8I GCNNGC 4 cut(s) 16, 75, 120, 177
CaiI CAGNNNCTG 2 cut(s) 11, 197
Cfr13I GGNCC 2 cut(s) 8, 257
Csp6I GTAC 2 cut(s) 215, 515
CviAII CATG 2 cut(s) 62, 443
CviQI GTAC 2 cut(s) 215, 515
DdeI CTNAG 2 cut(s) 525, 591
DpnI GATC 2 cut(s) 311, 534
DpnII GATC 2 cut(s) 309, 532
DrdI GACNNNNNNGTC 1 cut(s) 92
DseDI GACNNNNNNGTC 1 cut(s) 92
EaeI YGGCCR 1 cut(s) 58
Eam1104I CTCTTC 2 cut(s) 277, 564
EarI CTCTTC 2 cut(s) 277, 564
Eco130I CCWWGG 1 cut(s) 402
Eco47I GGWCC 1 cut(s) 257
Eco57I CTGAAG 1 cut(s) 342
EcoO109I RGGNCCY 1 cut(s) 8
EcoRII CCWGG 1 cut(s) 244
EcoT14I CCWWGG 1 cut(s) 402
ErhI CCWWGG 1 cut(s) 402
FaeI CATG 2 cut(s) 65, 446
FaiI YATR 7 cut(s) 63, 93, 153, 231, 429, 444, 611
FatI CATG 2 cut(s) 61, 442
Fnu4HI GCNGC 6 cut(s) 19, 192, 195, 327, 408, 447
FokI GGATG 1 cut(s) 447
Fsp4HI GCNGC 6 cut(s) 19, 192, 195, 327, 408, 447
GluI GCNGC 6 cut(s) 19, 192, 195, 327, 408, 447
GsaI CCCAGC 2 cut(s) 47, 201
GsuI CTGGAG 1 cut(s) 15
HaeIII GGCC 2 cut(s) 9, 60
HapII CCGG 1 cut(s) 57
Hin1I GRCGYC 1 cut(s) 552
Hin1II CATG 2 cut(s) 65, 446
HindIII AAGCTT 2 cut(s) 71, 116
HinfI GANTC 2 cut(s) 86, 110
HpaII CCGG 1 cut(s) 57
HphI GGTGA 2 cut(s) 260, 611
Hpy166II GTNNAC 1 cut(s) 481
Hpy188I TCNGA 2 cut(s) 322, 537
Hpy188III TCNNGA 4 cut(s) 83, 107, 166, 556
Hpy8I GTNNAC 1 cut(s) 481
Hpy99I CGWCG 1 cut(s) 557
HpyAV CCTTC 2 cut(s) 446, 474
HpyCH4III ACNGT 1 cut(s) 478
HpyCH4IV ACGT 1 cut(s) 552
HpyCH4V TGCA 5 cut(s) 191, 329, 371, 422, 584
HpyF10VI GCNNNNNNNGC 4 cut(s) 15, 323, 377, 407
HpyF3I CTNAG 2 cut(s) 525, 591
HpySE526I ACGT 1 cut(s) 552
Hsp92I GRCGYC 1 cut(s) 552
Hsp92II CATG 2 cut(s) 65, 446
KpnI GGTACC 1 cut(s) 218
Kzo9I GATC 2 cut(s) 309, 532
LmnI GCTCC 3 cut(s) 163, 398, 521
Lsp1109I GCAGC 6 cut(s) 5, 181, 203, 313, 394, 433
LweI GCATC 3 cut(s) 226, 241, 326
MaeII ACGT 1 cut(s) 552
MaeIII GTNAC 2 cut(s) 94, 472
MalI GATC 2 cut(s) 311, 534
MboI GATC 2 cut(s) 309, 532
MboII GAAGA 3 cut(s) 119, 264, 581
MfeI CAATTG 1 cut(s) 372
MluCI AATT 4 cut(s) 372, 563, 602, 616
MlyI GAGTC 2 cut(s) 80, 104
MmeI TCCRAC 1 cut(s) 244
MnlI CCTC 3 cut(s) 39, 162, 565
MroXI GAANNNNTTC 1 cut(s) 271
MseI TTAA 5 cut(s) 39, 293, 350, 498, 578
MspA1I CMGCKG 1 cut(s) 194
MspI CCGG 1 cut(s) 57
MspR9I CCNGG 1 cut(s) 246
MunI CAATTG 1 cut(s) 372
MvaI CCWGG 1 cut(s) 246
MwoI GCNNNNNNNGC 4 cut(s) 15, 323, 377, 407
NdeII GATC 2 cut(s) 309, 532
NlaIII CATG 2 cut(s) 65, 446
NlaIV GGNNCC 4 cut(s) 55, 216, 400, 504
NmuCI GTSAC 2 cut(s) 94, 472
PdmI GAANNNNTTC 1 cut(s) 271
PflMI CCANNNNNTGG 1 cut(s) 50
PkrI GCNGC 6 cut(s) 20, 193, 196, 328, 409, 448
PleI GAGTC 2 cut(s) 80, 104
PpsI GAGTC 2 cut(s) 80, 104
Psp6I CCWGG 1 cut(s) 244
PspFI CCCAGC 2 cut(s) 43, 197
PspGI CCWGG 1 cut(s) 244
PspN4I GGNNCC 4 cut(s) 55, 216, 400, 504
PspPI GGNCC 2 cut(s) 8, 257
PstNI CAGNNNCTG 2 cut(s) 11, 197
PvuII CAGCTG 1 cut(s) 194
RsaI GTAC 2 cut(s) 216, 516
RsaNI GTAC 2 cut(s) 215, 515
SaqAI TTAA 5 cut(s) 39, 293, 350, 498, 578
SatI GCNGC 6 cut(s) 19, 192, 195, 327, 408, 447
Sau3AI GATC 2 cut(s) 309, 532
Sau96I GGNCC 2 cut(s) 8, 257
SchI GAGTC 2 cut(s) 80, 104
ScrFI CCNGG 1 cut(s) 246
SfaNI GCATC 3 cut(s) 226, 241, 326
SfcI CTRYAG 1 cut(s) 381
SinI GGWCC 1 cut(s) 257
SmlI CTYRAG 1 cut(s) 166
SmoI CTYRAG 1 cut(s) 166
Sse9I AATT 4 cut(s) 372, 563, 602, 616
SsiI CCGC 2 cut(s) 410, 494
StyD4I CCNGG 1 cut(s) 244
StyI CCWWGG 1 cut(s) 402
TaaI ACNGT 1 cut(s) 478
TaiI ACGT 1 cut(s) 555
TasI AATT 4 cut(s) 372, 563, 602, 616
TatI WGTACW 1 cut(s) 514
Tru1I TTAA 5 cut(s) 39, 293, 350, 498, 578
Tru9I TTAA 5 cut(s) 39, 293, 350, 498, 578
TscAI CASTG 2 cut(s) 103, 481
TseFI GTSAC 2 cut(s) 94, 472
TseI GCWGC 6 cut(s) 18, 191, 194, 326, 407, 446
Tsp45I GTSAC 2 cut(s) 94, 472
TspDTI ATGAA 2 cut(s) 168, 456
TspRI CASTG 2 cut(s) 103, 481
Van91I CCANNNNNTGG 1 cut(s) 50
VpaK11BI GGWCC 1 cut(s) 257
XmnI GAANNNNTTC 1 cut(s) 271
ZraI GACGTC 1 cut(s) 553
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.