Rh4BG214400

1,4-beta-D-glucanase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000177
Physical Location & Seq
Reverse (-)
37282261 .. 37285072
2812 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG214400.1

Sequence Viewer

Length: 726 bp
ATGTCAGGCCCTCAGTGCTGCTCGAACCCACCGACCCTGAACCCAACCAGTGGATCCGGCCACGTTGAGAAGCTTGGTGGTCTCGACTCCTATCTCACTGGCTCTCCTGACTCCAAGCTTGCCATTGTTCTTGTCTCTGACGTTTATGGGTATAATGCTCCAAACTTGAGGAAGCTCGCAGACAAAGTTGCAGCTGCTGGCTTCTTTGTTGTGGTCCCTGACTTCTTTTATGGAGACCCTTATGTACCTCCAGCTGATGGATCCCATGGCGGTCTACCTACATGGCTGAAAAGTCATGAAGCGGAGAAGGGATTTGAAGATGCTAAAGTAGTTATTGAAGCTCTAAAAAGTAAAGGGGTTTCTGCAATCGGTGCTGCAGGCTTCTGTTGGGGAGCCAAGGTTGTAGTTGAACTTGCAAAGGGTGACTTTATCCAAGCTGCTGTTCTGGCTCATCCTTCTTTTGTCACTGTGGATGATATCAAGGCTGTTAAGGTTCCTATATCTATACTTGGAGCTGAGATTGATCGGATGTCTCCGCCTGAAGTTGTGAAACAATTTGAAGAGGCGTTGACTGCAAAGTCTGAGATCAAAAGTCATGTGAAGATATTCCCAAAAGTGTCGCATGGATGGACTGTGAGGTATGATGTTTCAGACGAAGAAGCTGTGAAGCCCGCTGAGGAGGCTCACAAAGACATGTTGGAGTGGTTCGTGAACCATGTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
ko00361 ko00364 ko00623 ko01100 ko01110 ko01120 ko01130 map00361 map00364 map00623 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
DLH

Protein Analysis

241

Amino Acids

25.82

Weight (kDa)

5.61

Isoelectric Point (pI)

29.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DLH PF01738 29 - 238 6e-32 Dienelactone hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000177)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23570 AT3G23570 AT3G23570 AT3G23600 AT3G23600
fragaria_vesca FvH4_2g23960 FvH4_2g23960 FvH4_2g23960 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14330 FvH4_6g28031 FvH4_6g29270
malus_domestica MD13G1181200.v1.1 MD13G1181300.v1.1 MD13G1181400.v1.1 MD13G1181500.v1.1 MD13G1181600.v1.1 MD13G1181700.v1.1 MD13G1181800.v1.1 MD13G1181900.v1.1 MD15G1403300.v1.1 MD15G1403400.v1.1 MD16G1181900.v1.1 MD16G1182000.v1.1 MD16G1182200.v1.1 MD16G1182300.v1.1 MD16G1182400.v1.1
prunus_persica Prupe.1G149400_v2.0.a1 Prupe.1G149600_v2.0.a1 Prupe.1G149700_v2.0.a1 Prupe.1G149800_v2.0.a1 Prupe.1G549400_v2.0.a1 Prupe.8G245600_v2.0.a1
pyrus_communis pycom13g15640 pycom13g15650 pycom13g15670 pycom13g15700 pycom15g36070 pycom16g15270 pycom16g15300 pycom16g15310
rosa_chinensis RchiOBHm_Chr1g0334461 RchiOBHm_Chr1g0334471 RchiOBHm_Chr2g0131731 RchiOBHm_Chr4g0417881 RchiOBHm_Chr4g0418561 RchiOBHm_Chr4g0418571 RchiOBHm_Chr4g0418581 RchiOBHm_Chr4g0418591 RchiOBHm_Chr4g0418601 RchiOBHm_Chr4g0418621 RchiOBHm_Chr4g0418651 RchiOBHm_Chr4g0418661 RchiOBHm_Chr4g0418671 RchiOBHm_Chr4g0418681 RchiOBHm_Chr4g0418691 RchiOBHm_Chr4g0418701 RchiOBHm_Chr4g0421371 RchiOBHm_Chr6g0291601 RchiOBHm_Chr6g0291611 RchiOBHm_Chr6g0291621 RchiOBHm_Chr6g0291631 RchiOBHm_Chr7g0229821
rosa_laevigata RLG00000007843 RLG00000007844 RLG00000007846 RLG00000007847 RLG00000007849 RLG00000012101 RLG00000012104 RLG00000029451
rosa_multiflora Rmu_sc0000363.1_g000035 Rmu_sc0000935.1_g000003 Rmu_sc0000935.1_g000010 Rmu_sc0002226.1_g000008 Rmu_sc0002226.1_g000014 Rmu_sc0002589.1_g000031 Rmu_sc0003443.1_g000009 Rmu_sc0006431.1_g000001 Rmu_sc0006431.1_g000006 Rmu_sc0006431.1_g000023 Rmu_sc0006705.1_g000014 Rmu_sc0006705.1_g000015 Rmu_sc0006705.1_g000016 Rmu_sc0006705.1_g000017 Rmu_sc0006705.1_g000018 Rmu_sc0014797.1_g000001 Rmu_sc0041885.1_g000001
rosa_roxburghii Rroxscaffold_4G00316820 Rroxscaffold_5G00361830 Rroxscaffold_5G00361840 Rroxscaffold_5G00361850 Rroxscaffold_5G00361860 Rroxscaffold_5G00361880 Rroxscaffold_5G00361890 Rroxscaffold_5G00361900 Rroxscaffold_5G00361910 Rroxscaffold_5G00361920 Rroxscaffold_5G00361930 Rroxscaffold_7G00175910 Rroxscaffold_7G00175930 Rroxscaffold_7G00175940
rosa_rugosa Rorug02G0299300 Rorug04G0154500 Rorug04G0154600 Rorug04G0154700 Rorug06G0220500
rosa_samantha Rh1AG006300 Rh1AG139200 Rh1AG139300 Rh1BG105600 Rh1CG131200 Rh1CG131300 Rh1DG143200 Rh2AG210000 Rh2CG337500 Rh2DG216200 Rh2DG376200 Rh4AG215900 Rh4AG216000 Rh4AG216100 Rh4AG216200 Rh4AG216300 Rh4AG216400 Rh4BG214300 Rh4BG214400 Rh4BG214500 Rh4BG214600 Rh4BG214700 Rh4BG214800 Rh4CG212300 Rh4CG227500 Rh4CG227700 Rh4CG227900 Rh4CG228000 Rh4CG228100 Rh4DG214200 Rh4DG215000 Rh4DG215200 Rh5AG059400 Rh6AG332900 Rh6AG333000 Rh6AG333100 Rh6BG339700 Rh6BG339900 Rh6CG346100 Rh6CG346200 Rh6DG333300 Rh6DG333400 Rh7AG435400 Rh7CG454500
rosa_wichuraiana Rw1G011480 Rw2G028580 Rw4G018270 Rw4G018280 Rw4G018290 Rw4G018360 Rw4G018390 Rw4G018400 Rw4G018410 Rw4G018420 Rw6G028910 Rw6G029000 Rw6G029010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 577
AccB7I CCANNNNNTGG 2 cut(s) 50, 257
AccI GTMKAC 1 cut(s) 274
AciI CCGC 4 cut(s) 270, 302, 536, 672
AclWI GGATC 4 cut(s) 48, 61, 255, 268
AcoI YGGCCR 1 cut(s) 58
AcuI CTGAAG 1 cut(s) 561
AfaI GTAC 1 cut(s) 246
AfiI CCNNNNNNNGG 2 cut(s) 50, 257
AflIII ACRYGT 1 cut(s) 693
AgsI TTSAA 4 cut(s) 317, 338, 410, 560
AjuI GAANNNNNNNTTGG 2 cut(s) 426, 458
AluBI AGCT 9 cut(s) 73, 118, 175, 194, 254, 341, 437, 515, 662
AluI AGCT 9 cut(s) 73, 118, 175, 194, 254, 341, 437, 515, 662
Alw26I GTCTC 4 cut(s) 86, 139, 228, 537
AlwI GGATC 4 cut(s) 48, 61, 255, 268
AlwNI CAGNNNCTG 1 cut(s) 197
AoxI GGCC 2 cut(s) 7, 58
ApeKI GCWGC 5 cut(s) 18, 191, 194, 374, 437
Asp700I GAANNNNTTC 1 cut(s) 605
AspS9I GGNCC 2 cut(s) 8, 214
AsuHPI GGTGA 1 cut(s) 434
AvaII GGWCC 1 cut(s) 214
BamHI GGATCC 2 cut(s) 53, 260
BbvCI CCTCAGC 1 cut(s) 675
BbvI GCAGC 5 cut(s) 5, 181, 203, 361, 424
BccI CCATC 2 cut(s) 251, 621
BcoDI GTCTC 4 cut(s) 86, 139, 228, 537
BfmI CTRYAG 1 cut(s) 375
BisI GCNGC 5 cut(s) 19, 192, 195, 375, 438
BlsI GCNGC 5 cut(s) 20, 193, 196, 376, 439
Bme18I GGWCC 1 cut(s) 214
BmgT120I GGNCC 2 cut(s) 8, 214
BmiI GGNNCC 5 cut(s) 55, 216, 262, 394, 495
BmsI GCATC 1 cut(s) 310
BpmI CTGGAG 1 cut(s) 234
Bpu10I CCTNAGC 1 cut(s) 675
BpuEI CTTGAG 1 cut(s) 187
BsaI GGTCTC 2 cut(s) 86, 228
BsaJI CCNNGG 2 cut(s) 265, 396
BsaXI ACNNNNNCTCC 4 cut(s) 88, 118, 384, 414
Bsc4I CCNNNNNNNGG 2 cut(s) 50, 257
Bse1I ACTGG 2 cut(s) 48, 103
BseDI CCNNGG 2 cut(s) 265, 396
BseGI GGATG 4 cut(s) 451, 478, 534, 632
BseLI CCNNNNNNNGG 2 cut(s) 50, 257
BseMII CTCAG 4 cut(s) 26, 507, 573, 666
BseNI ACTGG 2 cut(s) 48, 103
BseRI GAGGAG 1 cut(s) 692
BseXI GCAGC 5 cut(s) 5, 181, 203, 361, 424
BshFI GGCC 2 cut(s) 9, 60
BsiSI CCGG 1 cut(s) 57
BslFI GGGAC 1 cut(s) 200
BslI CCNNNNNNNGG 2 cut(s) 50, 257
BsmAI GTCTC 4 cut(s) 86, 139, 228, 537
BsmFI GGGAC 1 cut(s) 200
BsnI GGCC 2 cut(s) 9, 60
Bso31I GGTCTC 2 cut(s) 86, 228
Bsp143I GATC 4 cut(s) 53, 260, 523, 585
Bsp19I CCATGG 1 cut(s) 265
BspACI CCGC 4 cut(s) 270, 302, 536, 672
BspANI GGCC 2 cut(s) 9, 60
BspCNI CTCAG 4 cut(s) 25, 508, 574, 667
BspHI TCATGA 1 cut(s) 295
BspLI GGNNCC 5 cut(s) 55, 216, 262, 394, 495
BspMAI CTGCAG 1 cut(s) 379
BspPI GGATC 4 cut(s) 48, 61, 255, 268
BspTNI GGTCTC 2 cut(s) 86, 228
BsrI ACTGG 2 cut(s) 48, 103
BssECI CCNNGG 2 cut(s) 265, 396
BssMI GATC 4 cut(s) 53, 260, 523, 585
BssT1I CCWWGG 2 cut(s) 265, 396
Bst4CI ACNGT 2 cut(s) 469, 634
Bst6I CTCTTC 1 cut(s) 555
BstAPI GCANNNNNTGC 1 cut(s) 371
BstC8I GCNNGC 5 cut(s) 120, 177, 199, 379, 672
BstDEI CTNAG 4 cut(s) 12, 516, 582, 675
BstDSI CCRYGG 1 cut(s) 265
BstF5I GGATG 4 cut(s) 451, 478, 534, 632
BstKTI GATC 4 cut(s) 56, 263, 526, 588
BstMAI GTCTC 4 cut(s) 86, 139, 228, 537
BstMBI GATC 4 cut(s) 53, 260, 523, 585
BstMWI GCNNNNNNNGC 5 cut(s) 15, 371, 446, 572, 680
BstNSI RCATGY 1 cut(s) 697
BstSFI CTRYAG 1 cut(s) 375
BstV1I GCAGC 5 cut(s) 5, 181, 203, 361, 424
BstX2I RGATCY 2 cut(s) 53, 260
BstYI RGATCY 2 cut(s) 53, 260
BsuRI GGCC 2 cut(s) 9, 60
BtgI CCRYGG 1 cut(s) 265
BtsCI GGATG 4 cut(s) 451, 478, 534, 632
BtsIMutI CAGTG 4 cut(s) 20, 55, 96, 465
Cac8I GCNNGC 5 cut(s) 120, 177, 199, 379, 672
CaiI CAGNNNCTG 1 cut(s) 197
CciI TCATGA 1 cut(s) 295
Cfr13I GGNCC 2 cut(s) 8, 214
Csp6I GTAC 1 cut(s) 245
CviAII CATG 7 cut(s) 266, 282, 296, 596, 623, 694, 716
CviQI GTAC 1 cut(s) 245
DdeI CTNAG 4 cut(s) 12, 516, 582, 675
DpnI GATC 4 cut(s) 55, 262, 525, 587
DpnII GATC 4 cut(s) 53, 260, 523, 585
DrdI GACNNNNNNGTC 1 cut(s) 577
DseDI GACNNNNNNGTC 1 cut(s) 577
EaeI YGGCCR 1 cut(s) 58
Eam1104I CTCTTC 1 cut(s) 555
EarI CTCTTC 1 cut(s) 555
EciI GGCGGA 1 cut(s) 525
Eco130I CCWWGG 2 cut(s) 265, 396
Eco31I GGTCTC 2 cut(s) 86, 228
Eco32I GATATC 1 cut(s) 478
Eco47I GGWCC 1 cut(s) 214
Eco57I CTGAAG 1 cut(s) 561
EcoO109I RGGNCCY 1 cut(s) 8
EcoRV GATATC 1 cut(s) 478
EcoT14I CCWWGG 2 cut(s) 265, 396
ErhI CCWWGG 2 cut(s) 265, 396
FaeI CATG 7 cut(s) 269, 285, 299, 599, 626, 697, 719
FalI AAGNNNNNCTT 2 cut(s) 410, 442
FaqI GGGAC 1 cut(s) 200
FatI CATG 7 cut(s) 265, 281, 295, 595, 622, 693, 715
FauI CCCGC 1 cut(s) 679
FblI GTMKAC 1 cut(s) 274
Fnu4HI GCNGC 5 cut(s) 19, 192, 195, 375, 438
FokI GGATG 4 cut(s) 438, 485, 541, 639
Fsp4HI GCNGC 5 cut(s) 19, 192, 195, 375, 438
GluI GCNGC 5 cut(s) 19, 192, 195, 375, 438
GsuI CTGGAG 1 cut(s) 234
HaeIII GGCC 2 cut(s) 9, 60
HapII CCGG 1 cut(s) 57
Hin1II CATG 7 cut(s) 269, 285, 299, 599, 626, 697, 719
HincII GTYRAC 1 cut(s) 570
HindII GTYRAC 1 cut(s) 570
HindIII AAGCTT 2 cut(s) 71, 116
HinfI GANTC 2 cut(s) 86, 110
HpaII CCGG 1 cut(s) 57
HphI GGTGA 1 cut(s) 434
Hpy166II GTNNAC 3 cut(s) 275, 570, 712
Hpy188I TCNGA 4 cut(s) 139, 528, 583, 652
Hpy188III TCNNGA 4 cut(s) 83, 107, 296, 709
Hpy8I GTNNAC 3 cut(s) 275, 570, 712
HpyAV CCTTC 2 cut(s) 301, 465
HpyCH4III ACNGT 2 cut(s) 469, 634
HpyCH4IV ACGT 2 cut(s) 63, 141
HpyCH4V TGCA 5 cut(s) 191, 365, 377, 416, 575
HpyF10VI GCNNNNNNNGC 5 cut(s) 15, 371, 446, 572, 680
HpyF3I CTNAG 4 cut(s) 12, 516, 582, 675
HpySE526I ACGT 2 cut(s) 63, 141
Hsp92II CATG 7 cut(s) 269, 285, 299, 599, 626, 697, 719
Kzo9I GATC 4 cut(s) 53, 260, 523, 585
LmnI GCTCC 3 cut(s) 163, 392, 512
Lsp1109I GCAGC 5 cut(s) 5, 181, 203, 361, 424
LweI GCATC 1 cut(s) 310
MaeII ACGT 2 cut(s) 63, 141
MaeIII GTNAC 2 cut(s) 422, 463
MalI GATC 4 cut(s) 55, 262, 525, 587
MboI GATC 4 cut(s) 53, 260, 523, 585
MboII GAAGA 4 cut(s) 329, 572, 613, 668
MflI RGATCY 2 cut(s) 53, 260
MluCI AATT 1 cut(s) 554
MlyI GAGTC 2 cut(s) 80, 104
MmeI TCCRAC 1 cut(s) 678
MnlI CCTC 7 cut(s) 21, 162, 258, 556, 630, 670, 673
MroXI GAANNNNTTC 1 cut(s) 605
MseI TTAA 1 cut(s) 489
MspA1I CMGCKG 3 cut(s) 194, 254, 674
MspI CCGG 1 cut(s) 57
MwoI GCNNNNNNNGC 5 cut(s) 15, 371, 446, 572, 680
NcoI CCATGG 1 cut(s) 265
NdeII GATC 4 cut(s) 53, 260, 523, 585
NlaIII CATG 7 cut(s) 269, 285, 299, 599, 626, 697, 719
NlaIV GGNNCC 5 cut(s) 55, 216, 262, 394, 495
NmuCI GTSAC 2 cut(s) 422, 463
NspI RCATGY 1 cut(s) 697
PagI TCATGA 1 cut(s) 295
PciI ACATGT 1 cut(s) 693
PcsI WCGNNNNNNNCGW 1 cut(s) 29
PdmI GAANNNNTTC 1 cut(s) 605
PflMI CCANNNNNTGG 2 cut(s) 50, 257
PkrI GCNGC 5 cut(s) 20, 193, 196, 376, 439
PleI GAGTC 2 cut(s) 80, 104
PpsI GAGTC 2 cut(s) 80, 104
PscI ACATGT 1 cut(s) 693
PspN4I GGNNCC 5 cut(s) 55, 216, 262, 394, 495
PspPI GGNCC 2 cut(s) 8, 214
PstI CTGCAG 1 cut(s) 379
PstNI CAGNNNCTG 1 cut(s) 197
PsuI RGATCY 2 cut(s) 53, 260
PvuII CAGCTG 2 cut(s) 194, 254
RsaI GTAC 1 cut(s) 246
RsaNI GTAC 1 cut(s) 245
SaqAI TTAA 1 cut(s) 489
SatI GCNGC 5 cut(s) 19, 192, 195, 375, 438
Sau3AI GATC 4 cut(s) 53, 260, 523, 585
Sau96I GGNCC 2 cut(s) 8, 214
SchI GAGTC 2 cut(s) 80, 104
SfaNI GCATC 1 cut(s) 310
SfcI CTRYAG 1 cut(s) 375
SinI GGWCC 1 cut(s) 214
SmlI CTYRAG 1 cut(s) 166
SmoI CTYRAG 1 cut(s) 166
Sse9I AATT 1 cut(s) 554
SsiI CCGC 4 cut(s) 270, 302, 536, 672
StyI CCWWGG 2 cut(s) 265, 396
TaaI ACNGT 2 cut(s) 469, 634
TaiI ACGT 2 cut(s) 66, 144
TaqI TCGA 2 cut(s) 23, 84
TasI AATT 1 cut(s) 554
Tru1I TTAA 1 cut(s) 489
Tru9I TTAA 1 cut(s) 489
TscAI CASTG 4 cut(s) 20, 55, 103, 472
TseFI GTSAC 2 cut(s) 422, 463
TseI GCWGC 5 cut(s) 18, 191, 194, 374, 437
Tsp45I GTSAC 2 cut(s) 422, 463
TspDTI ATGAA 1 cut(s) 312
TspRI CASTG 4 cut(s) 20, 55, 103, 472
Van91I CCANNNNNTGG 2 cut(s) 50, 257
VpaK11BI GGWCC 1 cut(s) 214
XceI RCATGY 1 cut(s) 697
XmiI GTMKAC 1 cut(s) 274
XmnI GAANNNNTTC 1 cut(s) 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.