Rh4BG214500

1,4-beta-D-glucanase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000177
Physical Location & Seq
Reverse (-)
37289800 .. 37374554
84755 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG214500.1

Sequence Viewer

Length: 504 bp
ATGTCAGGCCCTCAGTGCTGCTCGAACCCACCGACCCTGAACCCAACCAGTGGATCCGGCCACATTGAGAAGCTTGGTGGTCTTGACTCCTATCTCACTGGCTCCCCTGACTCCAAGTTTGCCATTGTTCTTGTCTCCGACATTTTTGGGTATGATGCTCCAAACTTGAGGAAGCTCGCAGATAAAGTTGCAGCTGCTGGCTTCTTTGTTGTGGTCCCTGACTTCTTTTATGGAAACCCTTATGTACCTCCAGCTGATGGATCCCATGGCGGTATACCTACATGGCTGAAGGGTCATGGACCGGTTGTAGTTGAACTTGCAAAGGGTGACTTTATCCAAGCTGCTGTTCTGGCTCATCCTTCATTTGTCACTGTGGATGATATCAAGGCTGATGGAATAGTAGCTGCTACATCCATGGGAATATGTTATGATGATGAAAGCACAAAACAAATCCACACAGGCAAACTCCTCCTCTGTACACCCACAGACCACCTGCATCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
ko00361 ko00364 ko00623 ko01100 ko01110 ko01120 ko01130 map00361 map00364 map00623 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
DLH

Protein Analysis

167

Amino Acids

17.52

Weight (kDa)

5.28

Isoelectric Point (pI)

27.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DLH PF01738 29 - 79 2.1e-07 Dienelactone hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000177)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23570 AT3G23570 AT3G23570 AT3G23600 AT3G23600
fragaria_vesca FvH4_2g23960 FvH4_2g23960 FvH4_2g23960 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14330 FvH4_6g28031 FvH4_6g29270
malus_domestica MD13G1181200.v1.1 MD13G1181300.v1.1 MD13G1181400.v1.1 MD13G1181500.v1.1 MD13G1181600.v1.1 MD13G1181700.v1.1 MD13G1181800.v1.1 MD13G1181900.v1.1 MD15G1403300.v1.1 MD15G1403400.v1.1 MD16G1181900.v1.1 MD16G1182000.v1.1 MD16G1182200.v1.1 MD16G1182300.v1.1 MD16G1182400.v1.1
prunus_persica Prupe.1G149400_v2.0.a1 Prupe.1G149600_v2.0.a1 Prupe.1G149700_v2.0.a1 Prupe.1G149800_v2.0.a1 Prupe.1G549400_v2.0.a1 Prupe.8G245600_v2.0.a1
pyrus_communis pycom13g15640 pycom13g15650 pycom13g15670 pycom13g15700 pycom15g36070 pycom16g15270 pycom16g15300 pycom16g15310
rosa_chinensis RchiOBHm_Chr1g0334461 RchiOBHm_Chr1g0334471 RchiOBHm_Chr2g0131731 RchiOBHm_Chr4g0417881 RchiOBHm_Chr4g0418561 RchiOBHm_Chr4g0418571 RchiOBHm_Chr4g0418581 RchiOBHm_Chr4g0418591 RchiOBHm_Chr4g0418601 RchiOBHm_Chr4g0418621 RchiOBHm_Chr4g0418651 RchiOBHm_Chr4g0418661 RchiOBHm_Chr4g0418671 RchiOBHm_Chr4g0418681 RchiOBHm_Chr4g0418691 RchiOBHm_Chr4g0418701 RchiOBHm_Chr4g0421371 RchiOBHm_Chr6g0291601 RchiOBHm_Chr6g0291611 RchiOBHm_Chr6g0291621 RchiOBHm_Chr6g0291631 RchiOBHm_Chr7g0229821
rosa_laevigata RLG00000007843 RLG00000007844 RLG00000007846 RLG00000007847 RLG00000007849 RLG00000012101 RLG00000012104 RLG00000029451
rosa_multiflora Rmu_sc0000363.1_g000035 Rmu_sc0000935.1_g000003 Rmu_sc0000935.1_g000010 Rmu_sc0002226.1_g000008 Rmu_sc0002226.1_g000014 Rmu_sc0002589.1_g000031 Rmu_sc0003443.1_g000009 Rmu_sc0006431.1_g000001 Rmu_sc0006431.1_g000006 Rmu_sc0006431.1_g000023 Rmu_sc0006705.1_g000014 Rmu_sc0006705.1_g000015 Rmu_sc0006705.1_g000016 Rmu_sc0006705.1_g000017 Rmu_sc0006705.1_g000018 Rmu_sc0014797.1_g000001 Rmu_sc0041885.1_g000001
rosa_roxburghii Rroxscaffold_4G00316820 Rroxscaffold_5G00361830 Rroxscaffold_5G00361840 Rroxscaffold_5G00361850 Rroxscaffold_5G00361860 Rroxscaffold_5G00361880 Rroxscaffold_5G00361890 Rroxscaffold_5G00361900 Rroxscaffold_5G00361910 Rroxscaffold_5G00361920 Rroxscaffold_5G00361930 Rroxscaffold_7G00175910 Rroxscaffold_7G00175930 Rroxscaffold_7G00175940
rosa_rugosa Rorug02G0299300 Rorug04G0154500 Rorug04G0154600 Rorug04G0154700 Rorug06G0220500
rosa_samantha Rh1AG006300 Rh1AG139200 Rh1AG139300 Rh1BG105600 Rh1CG131200 Rh1CG131300 Rh1DG143200 Rh2AG210000 Rh2CG337500 Rh2DG216200 Rh2DG376200 Rh4AG215900 Rh4AG216000 Rh4AG216100 Rh4AG216200 Rh4AG216300 Rh4AG216400 Rh4BG214300 Rh4BG214400 Rh4BG214500 Rh4BG214600 Rh4BG214700 Rh4BG214800 Rh4CG212300 Rh4CG227500 Rh4CG227700 Rh4CG227900 Rh4CG228000 Rh4CG228100 Rh4DG214200 Rh4DG215000 Rh4DG215200 Rh5AG059400 Rh6AG332900 Rh6AG333000 Rh6AG333100 Rh6BG339700 Rh6BG339900 Rh6CG346100 Rh6CG346200 Rh6DG333300 Rh6DG333400 Rh7AG435400 Rh7CG454500
rosa_wichuraiana Rw1G011480 Rw2G028580 Rw4G018270 Rw4G018280 Rw4G018290 Rw4G018360 Rw4G018390 Rw4G018400 Rw4G018410 Rw4G018420 Rw6G028910 Rw6G029000 Rw6G029010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 50, 257
AccI GTMKAC 1 cut(s) 274
AciI CCGC 1 cut(s) 270
AclWI GGATC 4 cut(s) 48, 61, 255, 268
AcoI YGGCCR 1 cut(s) 58
AcuI CTGAAG 1 cut(s) 308
AfaI GTAC 2 cut(s) 246, 478
AfiI CCNNNNNNNGG 2 cut(s) 50, 257
AgeI ACCGGT 1 cut(s) 301
AgsI TTSAA 1 cut(s) 314
AjuI GAANNNNNNNTTGG 2 cut(s) 330, 362
AluBI AGCT 6 cut(s) 73, 175, 194, 254, 341, 404
AluI AGCT 6 cut(s) 73, 175, 194, 254, 341, 404
Alw26I GTCTC 1 cut(s) 139
AlwI GGATC 4 cut(s) 48, 61, 255, 268
AlwNI CAGNNNCTG 1 cut(s) 197
AoxI GGCC 2 cut(s) 7, 58
ApeKI GCWGC 5 cut(s) 18, 191, 194, 341, 404
ArsI GACNNNNNNTTYG 2 cut(s) 101, 133
AsiGI ACCGGT 1 cut(s) 301
AspS9I GGNCC 3 cut(s) 8, 214, 299
AsuHPI GGTGA 1 cut(s) 338
AvaII GGWCC 2 cut(s) 214, 299
BamHI GGATCC 2 cut(s) 53, 260
BbvI GCAGC 5 cut(s) 5, 181, 203, 328, 391
BccI CCATC 2 cut(s) 251, 386
BcoDI GTCTC 1 cut(s) 139
BisI GCNGC 5 cut(s) 19, 192, 195, 342, 405
BlsI GCNGC 5 cut(s) 20, 193, 196, 343, 406
Bme18I GGWCC 2 cut(s) 214, 299
BmgT120I GGNCC 3 cut(s) 8, 214, 299
BmiI GGNNCC 4 cut(s) 55, 103, 216, 262
BmsI GCATC 1 cut(s) 145
BpmI CTGGAG 1 cut(s) 234
BpuEI CTTGAG 1 cut(s) 187
BsaJI CCNNGG 2 cut(s) 265, 414
BsaWI WCCGGW 1 cut(s) 301
Bsc4I CCNNNNNNNGG 2 cut(s) 50, 257
Bse118I RCCGGY 1 cut(s) 301
Bse1I ACTGG 2 cut(s) 48, 103
BseDI CCNNGG 2 cut(s) 265, 414
BseGI GGATG 3 cut(s) 355, 382, 410
BseLI CCNNNNNNNGG 2 cut(s) 50, 257
BseMII CTCAG 1 cut(s) 26
BseNI ACTGG 2 cut(s) 48, 103
BseRI GAGGAG 2 cut(s) 458, 461
BseXI GCAGC 5 cut(s) 5, 181, 203, 328, 391
BshFI GGCC 2 cut(s) 9, 60
BshTI ACCGGT 1 cut(s) 301
BsiSI CCGG 2 cut(s) 57, 302
BslFI GGGAC 1 cut(s) 200
BslI CCNNNNNNNGG 2 cut(s) 50, 257
BsmAI GTCTC 1 cut(s) 139
BsmFI GGGAC 1 cut(s) 200
BsnI GGCC 2 cut(s) 9, 60
Bsp1407I TGTACA 1 cut(s) 476
Bsp143I GATC 2 cut(s) 53, 260
Bsp19I CCATGG 2 cut(s) 265, 414
BspACI CCGC 1 cut(s) 270
BspANI GGCC 2 cut(s) 9, 60
BspCNI CTCAG 1 cut(s) 25
BspLI GGNNCC 4 cut(s) 55, 103, 216, 262
BspPI GGATC 4 cut(s) 48, 61, 255, 268
BsrFI RCCGGY 1 cut(s) 301
BsrGI TGTACA 1 cut(s) 476
BsrI ACTGG 2 cut(s) 48, 103
BssAI RCCGGY 1 cut(s) 301
BssECI CCNNGG 2 cut(s) 265, 414
BssMI GATC 2 cut(s) 53, 260
BssNAI GTATAC 1 cut(s) 275
BssT1I CCWWGG 2 cut(s) 265, 414
Bst1107I GTATAC 1 cut(s) 275
Bst4CI ACNGT 1 cut(s) 373
BstAUI TGTACA 1 cut(s) 476
BstC8I GCNNGC 2 cut(s) 177, 199
BstDEI CTNAG 1 cut(s) 12
BstDSI CCRYGG 2 cut(s) 265, 414
BstF5I GGATG 3 cut(s) 355, 382, 410
BstKTI GATC 2 cut(s) 56, 263
BstMAI GTCTC 1 cut(s) 139
BstMBI GATC 2 cut(s) 53, 260
BstMWI GCNNNNNNNGC 2 cut(s) 15, 350
BstV1I GCAGC 5 cut(s) 5, 181, 203, 328, 391
BstX2I RGATCY 2 cut(s) 53, 260
BstYI RGATCY 2 cut(s) 53, 260
BstZ17I GTATAC 1 cut(s) 275
BsuRI GGCC 2 cut(s) 9, 60
BtgI CCRYGG 2 cut(s) 265, 414
BtsCI GGATG 3 cut(s) 355, 382, 410
BtsIMutI CAGTG 4 cut(s) 20, 55, 96, 369
Cac8I GCNNGC 2 cut(s) 177, 199
CaiI CAGNNNCTG 1 cut(s) 197
Cfr10I RCCGGY 1 cut(s) 301
Cfr13I GGNCC 3 cut(s) 8, 214, 299
Csp6I GTAC 2 cut(s) 245, 477
CspAI ACCGGT 1 cut(s) 301
CviAII CATG 4 cut(s) 266, 282, 296, 415
CviQI GTAC 2 cut(s) 245, 477
DdeI CTNAG 1 cut(s) 12
DpnI GATC 2 cut(s) 55, 262
DpnII GATC 2 cut(s) 53, 260
EaeI YGGCCR 1 cut(s) 58
Eco130I CCWWGG 2 cut(s) 265, 414
Eco32I GATATC 1 cut(s) 382
Eco47I GGWCC 2 cut(s) 214, 299
Eco57I CTGAAG 1 cut(s) 308
EcoO109I RGGNCCY 1 cut(s) 8
EcoRV GATATC 1 cut(s) 382
EcoT14I CCWWGG 2 cut(s) 265, 414
ErhI CCWWGG 2 cut(s) 265, 414
FaeI CATG 4 cut(s) 269, 285, 299, 418
FalI AAGNNNNNCTT 2 cut(s) 314, 346
FaqI GGGAC 1 cut(s) 200
FatI CATG 4 cut(s) 265, 281, 295, 414
FblI GTMKAC 1 cut(s) 274
Fnu4HI GCNGC 5 cut(s) 19, 192, 195, 342, 405
FokI GGATG 3 cut(s) 342, 389, 397
Fsp4HI GCNGC 5 cut(s) 19, 192, 195, 342, 405
GluI GCNGC 5 cut(s) 19, 192, 195, 342, 405
GsuI CTGGAG 1 cut(s) 234
HaeIII GGCC 2 cut(s) 9, 60
HapII CCGG 2 cut(s) 57, 302
Hin1II CATG 4 cut(s) 269, 285, 299, 418
HindIII AAGCTT 1 cut(s) 71
HinfI GANTC 2 cut(s) 86, 110
HpaII CCGG 2 cut(s) 57, 302
HphI GGTGA 1 cut(s) 338
Hpy166II GTNNAC 2 cut(s) 275, 479
Hpy188I TCNGA 1 cut(s) 139
Hpy188III TCNNGA 1 cut(s) 83
Hpy8I GTNNAC 2 cut(s) 275, 479
HpyAV CCTTC 2 cut(s) 283, 369
HpyCH4III ACNGT 1 cut(s) 373
HpyCH4V TGCA 3 cut(s) 191, 320, 496
HpyF10VI GCNNNNNNNGC 2 cut(s) 15, 350
HpyF3I CTNAG 1 cut(s) 12
Hsp92II CATG 4 cut(s) 269, 285, 299, 418
Kzo9I GATC 2 cut(s) 53, 260
LmnI GCTCC 2 cut(s) 107, 163
Lsp1109I GCAGC 5 cut(s) 5, 181, 203, 328, 391
LweI GCATC 1 cut(s) 145
MaeIII GTNAC 2 cut(s) 326, 367
MalI GATC 2 cut(s) 55, 262
MboI GATC 2 cut(s) 53, 260
MflI RGATCY 2 cut(s) 53, 260
MlyI GAGTC 2 cut(s) 80, 104
MmeI TCCRAC 1 cut(s) 162
MnlI CCTC 5 cut(s) 21, 162, 258, 479, 482
MspA1I CMGCKG 2 cut(s) 194, 254
MspI CCGG 2 cut(s) 57, 302
MwoI GCNNNNNNNGC 2 cut(s) 15, 350
NcoI CCATGG 2 cut(s) 265, 414
NdeII GATC 2 cut(s) 53, 260
NlaIII CATG 4 cut(s) 269, 285, 299, 418
NlaIV GGNNCC 4 cut(s) 55, 103, 216, 262
NmuCI GTSAC 2 cut(s) 326, 367
PcsI WCGNNNNNNNCGW 1 cut(s) 29
PflMI CCANNNNNTGG 2 cut(s) 50, 257
PinAI ACCGGT 1 cut(s) 301
PkrI GCNGC 5 cut(s) 20, 193, 196, 343, 406
PleI GAGTC 2 cut(s) 80, 104
PpsI GAGTC 2 cut(s) 80, 104
PspN4I GGNNCC 4 cut(s) 55, 103, 216, 262
PspPI GGNCC 3 cut(s) 8, 214, 299
PstNI CAGNNNCTG 1 cut(s) 197
PsuI RGATCY 2 cut(s) 53, 260
PvuII CAGCTG 2 cut(s) 194, 254
RsaI GTAC 2 cut(s) 246, 478
RsaNI GTAC 2 cut(s) 245, 477
SatI GCNGC 5 cut(s) 19, 192, 195, 342, 405
Sau3AI GATC 2 cut(s) 53, 260
Sau96I GGNCC 3 cut(s) 8, 214, 299
SchI GAGTC 2 cut(s) 80, 104
SetI ASST 9 cut(s) 75, 177, 196, 250, 256, 280, 343, 406, 495
SfaNI GCATC 1 cut(s) 145
SinI GGWCC 2 cut(s) 214, 299
SmlI CTYRAG 1 cut(s) 166
SmoI CTYRAG 1 cut(s) 166
SsiI CCGC 1 cut(s) 270
StyI CCWWGG 2 cut(s) 265, 414
TaaI ACNGT 1 cut(s) 373
TaqI TCGA 1 cut(s) 23
TatI WGTACW 1 cut(s) 476
TscAI CASTG 4 cut(s) 20, 55, 103, 376
TseFI GTSAC 2 cut(s) 326, 367
TseI GCWGC 5 cut(s) 18, 191, 194, 341, 404
Tsp45I GTSAC 2 cut(s) 326, 367
TspDTI ATGAA 2 cut(s) 351, 450
TspRI CASTG 4 cut(s) 20, 55, 103, 376
Van91I CCANNNNNTGG 2 cut(s) 50, 257
VpaK11BI GGWCC 2 cut(s) 214, 299
XmiI GTMKAC 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.