Rh4AG216400

1,4-beta-D-glucanase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000177
Physical Location & Seq
Forward (+)
51280509 .. 51282863
2355 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG216400.1

Sequence Viewer

Length: 702 bp
ATGTCAGGCCCTGCGTGCTGCTCAAACCCTCCAGCCCTTAACCCAGCAAGTGGTTCCGGCCATGTTGAAAAGCTTGCTGGTCTGGACTCCTATGCCACTGGCTCTCCTGACTCCAAGCTTGCCGTTCTTCTCATTGCTGACATTTTTGACAAAGTTGCAGCTGCTGGGTTCTTTGTTGTGGTACCTGACTTCTTTTATGGAGATGCTTTTCCTGGTGATGCTGGACCAGTTGGAAACTCTTCTGCTCTACCTATTTGGTTAAAGACACATTTACCGATCAAGGCATCTGAAGCTGCAAAACCATTACTTGAAGCTTTAAAGAGTAAAGGTGTTTCTGCAATTGGGGCTGTAGGCTTCTGTTGGGGAGCCAAGGCTGCGGTTGAACTTGCAAAGTATGATGATCTTCTCCATGCTGCTGTCTTGTGCCATCCTTCATTCATCACTGTGGACGATTTCAAGGCGGTTAAGGTTCCCATTTCTGTACTTGGAGCTGAGATTGATCCGATTTCGCCACCTGACGTCGTGAAACAATTTGAAGAGGTTTTAACTGCAAGACCTGAGATCAAAAGCCATGTGAAGATATTTCCAAAAGTGCAACATGGGTGGACCGTGAGGTACAACGCTGAAGATGAGGCCGCTGTTAAGTGTGCTGAGGAGGCTCATCAAGACATACTGGATTGGTTCTTTAGCCATGTCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
ko00361 ko00364 ko00623 ko01100 ko01110 ko01120 ko01130 map00361 map00364 map00623 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
DLH

Protein Analysis

233

Amino Acids

24.7

Weight (kDa)

5.43

Isoelectric Point (pI)

34.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DLH PF01738 45 - 229 5.8e-27 Dienelactone hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000177)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23570 AT3G23570 AT3G23570 AT3G23600 AT3G23600
fragaria_vesca FvH4_2g23960 FvH4_2g23960 FvH4_2g23960 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14330 FvH4_6g28031 FvH4_6g29270
malus_domestica MD13G1181200.v1.1 MD13G1181300.v1.1 MD13G1181400.v1.1 MD13G1181500.v1.1 MD13G1181600.v1.1 MD13G1181700.v1.1 MD13G1181800.v1.1 MD13G1181900.v1.1 MD15G1403300.v1.1 MD15G1403400.v1.1 MD16G1181900.v1.1 MD16G1182000.v1.1 MD16G1182200.v1.1 MD16G1182300.v1.1 MD16G1182400.v1.1
prunus_persica Prupe.1G149400_v2.0.a1 Prupe.1G149600_v2.0.a1 Prupe.1G149700_v2.0.a1 Prupe.1G149800_v2.0.a1 Prupe.1G549400_v2.0.a1 Prupe.8G245600_v2.0.a1
pyrus_communis pycom13g15640 pycom13g15650 pycom13g15670 pycom13g15700 pycom15g36070 pycom16g15270 pycom16g15300 pycom16g15310
rosa_chinensis RchiOBHm_Chr1g0334461 RchiOBHm_Chr1g0334471 RchiOBHm_Chr2g0131731 RchiOBHm_Chr4g0417881 RchiOBHm_Chr4g0418561 RchiOBHm_Chr4g0418571 RchiOBHm_Chr4g0418581 RchiOBHm_Chr4g0418591 RchiOBHm_Chr4g0418601 RchiOBHm_Chr4g0418621 RchiOBHm_Chr4g0418651 RchiOBHm_Chr4g0418661 RchiOBHm_Chr4g0418671 RchiOBHm_Chr4g0418681 RchiOBHm_Chr4g0418691 RchiOBHm_Chr4g0418701 RchiOBHm_Chr4g0421371 RchiOBHm_Chr6g0291601 RchiOBHm_Chr6g0291611 RchiOBHm_Chr6g0291621 RchiOBHm_Chr6g0291631 RchiOBHm_Chr7g0229821
rosa_laevigata RLG00000007843 RLG00000007844 RLG00000007846 RLG00000007847 RLG00000007849 RLG00000012101 RLG00000012104 RLG00000029451
rosa_multiflora Rmu_sc0000363.1_g000035 Rmu_sc0000935.1_g000003 Rmu_sc0000935.1_g000010 Rmu_sc0002226.1_g000008 Rmu_sc0002226.1_g000014 Rmu_sc0002589.1_g000031 Rmu_sc0003443.1_g000009 Rmu_sc0006431.1_g000001 Rmu_sc0006431.1_g000006 Rmu_sc0006431.1_g000023 Rmu_sc0006705.1_g000014 Rmu_sc0006705.1_g000015 Rmu_sc0006705.1_g000016 Rmu_sc0006705.1_g000017 Rmu_sc0006705.1_g000018 Rmu_sc0014797.1_g000001 Rmu_sc0041885.1_g000001
rosa_roxburghii Rroxscaffold_4G00316820 Rroxscaffold_5G00361830 Rroxscaffold_5G00361840 Rroxscaffold_5G00361850 Rroxscaffold_5G00361860 Rroxscaffold_5G00361880 Rroxscaffold_5G00361890 Rroxscaffold_5G00361900 Rroxscaffold_5G00361910 Rroxscaffold_5G00361920 Rroxscaffold_5G00361930 Rroxscaffold_7G00175910 Rroxscaffold_7G00175930 Rroxscaffold_7G00175940
rosa_rugosa Rorug02G0299300 Rorug04G0154500 Rorug04G0154600 Rorug04G0154700 Rorug06G0220500
rosa_samantha Rh1AG006300 Rh1AG139200 Rh1AG139300 Rh1BG105600 Rh1CG131200 Rh1CG131300 Rh1DG143200 Rh2AG210000 Rh2CG337500 Rh2DG216200 Rh2DG376200 Rh4AG215900 Rh4AG216000 Rh4AG216100 Rh4AG216200 Rh4AG216300 Rh4AG216400 Rh4BG214300 Rh4BG214400 Rh4BG214500 Rh4BG214600 Rh4BG214700 Rh4BG214800 Rh4CG212300 Rh4CG227500 Rh4CG227700 Rh4CG227900 Rh4CG228000 Rh4CG228100 Rh4DG214200 Rh4DG215000 Rh4DG215200 Rh5AG059400 Rh6AG332900 Rh6AG333000 Rh6AG333100 Rh6BG339700 Rh6BG339900 Rh6CG346100 Rh6CG346200 Rh6DG333300 Rh6DG333400 Rh7AG435400 Rh7CG454500
rosa_wichuraiana Rw1G011480 Rw2G028580 Rw4G018270 Rw4G018280 Rw4G018290 Rw4G018360 Rw4G018390 Rw4G018400 Rw4G018410 Rw4G018420 Rw6G028910 Rw6G029000 Rw6G029010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 522
Acc65I GGTACC 1 cut(s) 181
AccB1I GGYRCC 1 cut(s) 181
AccB7I CCANNNNNTGG 1 cut(s) 50
AciI CCGC 3 cut(s) 377, 461, 636
AclWI GGATC 1 cut(s) 494
AcoI YGGCCR 1 cut(s) 58
AcuI CTGAAG 2 cut(s) 309, 645
AcyI GRCGYC 1 cut(s) 519
AfaI GTAC 3 cut(s) 183, 483, 617
AfiI CCNNNNNNNGG 1 cut(s) 50
AgsI TTSAA 5 cut(s) 68, 311, 383, 457, 536
AjnI CCWGG 1 cut(s) 211
AluBI AGCT 6 cut(s) 73, 118, 161, 293, 314, 491
AluI AGCT 6 cut(s) 73, 118, 161, 293, 314, 491
AlwI GGATC 1 cut(s) 494
AlwNI CAGNNNCTG 2 cut(s) 11, 164
AoxI GGCC 3 cut(s) 7, 58, 633
ApeKI GCWGC 6 cut(s) 18, 158, 161, 293, 374, 413
Asp700I GAANNNNTTC 1 cut(s) 238
Asp718I GGTACC 1 cut(s) 181
AspS9I GGNCC 3 cut(s) 8, 224, 606
AsuHPI GGTGA 1 cut(s) 227
AvaII GGWCC 2 cut(s) 224, 606
BanI GGYRCC 1 cut(s) 181
BbvCI CCTCAGC 1 cut(s) 651
BbvI GCAGC 6 cut(s) 5, 148, 170, 280, 361, 400
BccI CCATC 1 cut(s) 435
BceAI ACGGC 1 cut(s) 107
BciT130I CCWGG 1 cut(s) 213
BfmI CTRYAG 1 cut(s) 348
BisI GCNGC 7 cut(s) 19, 159, 162, 294, 375, 414, 636
BlsI GCNGC 7 cut(s) 20, 160, 163, 295, 376, 415, 637
Bme1390I CCNGG 1 cut(s) 213
Bme18I GGWCC 2 cut(s) 224, 606
BmgT120I GGNCC 3 cut(s) 8, 224, 606
BmiI GGNNCC 4 cut(s) 55, 183, 367, 471
BmrFI CCNGG 1 cut(s) 213
BmsI GCATC 3 cut(s) 193, 208, 293
BpmI CTGGAG 1 cut(s) 15
Bpu10I CCTNAGC 1 cut(s) 651
BsaHI GRCGYC 1 cut(s) 519
BsaJI CCNNGG 1 cut(s) 369
BsaXI ACNNNNNCTCC 2 cut(s) 88, 118
Bsc4I CCNNNNNNNGG 1 cut(s) 50
Bse1I ACTGG 3 cut(s) 103, 227, 678
Bse3DI GCAATG 1 cut(s) 132
BseBI CCWGG 1 cut(s) 213
BseDI CCNNGG 1 cut(s) 369
BseGI GGATG 1 cut(s) 427
BseLI CCNNNNNNNGG 1 cut(s) 50
BseMI GCAATG 1 cut(s) 132
BseMII CTCAG 3 cut(s) 483, 549, 642
BseNI ACTGG 3 cut(s) 103, 227, 678
BseRI GAGGAG 1 cut(s) 668
BseXI GCAGC 6 cut(s) 5, 148, 170, 280, 361, 400
BseYI CCCAGC 2 cut(s) 43, 164
BshFI GGCC 3 cut(s) 9, 60, 635
BshNI GGYRCC 1 cut(s) 181
BsiSI CCGG 1 cut(s) 57
BslI CCNNNNNNNGG 1 cut(s) 50
BsnI GGCC 3 cut(s) 9, 60, 635
Bsp143I GATC 4 cut(s) 276, 400, 499, 561
BspACI CCGC 3 cut(s) 377, 461, 636
BspANI GGCC 3 cut(s) 9, 60, 635
BspCNI CTCAG 3 cut(s) 484, 550, 643
BspLI GGNNCC 4 cut(s) 55, 183, 367, 471
BspPI GGATC 1 cut(s) 494
BspT107I GGYRCC 1 cut(s) 181
BsrDI GCAATG 1 cut(s) 132
BsrI ACTGG 3 cut(s) 103, 227, 678
BssECI CCNNGG 1 cut(s) 369
BssMI GATC 4 cut(s) 276, 400, 499, 561
BssNI GRCGYC 1 cut(s) 519
BssT1I CCWWGG 1 cut(s) 369
Bst2UI CCWGG 1 cut(s) 213
Bst4CI ACNGT 2 cut(s) 445, 610
Bst6I CTCTTC 2 cut(s) 244, 531
BstACI GRCGYC 1 cut(s) 519
BstC8I GCNNGC 3 cut(s) 16, 75, 120
BstDEI CTNAG 3 cut(s) 492, 558, 651
BstF5I GGATG 1 cut(s) 427
BstKTI GATC 4 cut(s) 279, 403, 502, 564
BstMBI GATC 4 cut(s) 276, 400, 499, 561
BstMWI GCNNNNNNNGC 5 cut(s) 15, 290, 344, 374, 656
BstNI CCWGG 1 cut(s) 213
BstSCI CCNGG 1 cut(s) 211
BstSFI CTRYAG 1 cut(s) 348
BstV1I GCAGC 6 cut(s) 5, 148, 170, 280, 361, 400
BsuRI GGCC 3 cut(s) 9, 60, 635
BtsCI GGATG 1 cut(s) 427
BtsIMutI CAGTG 2 cut(s) 96, 441
Cac8I GCNNGC 3 cut(s) 16, 75, 120
CaiI CAGNNNCTG 2 cut(s) 11, 164
Cfr13I GGNCC 3 cut(s) 8, 224, 606
Csp6I GTAC 3 cut(s) 182, 482, 616
CspCI CAANNNNNGTGG 2 cut(s) 584, 619
CviAII CATG 5 cut(s) 62, 410, 572, 599, 692
CviQI GTAC 3 cut(s) 182, 482, 616
DdeI CTNAG 3 cut(s) 492, 558, 651
DpnI GATC 4 cut(s) 278, 402, 501, 563
DpnII GATC 4 cut(s) 276, 400, 499, 561
DraI TTTAAA 1 cut(s) 318
EaeI YGGCCR 1 cut(s) 58
Eam1104I CTCTTC 2 cut(s) 244, 531
EarI CTCTTC 2 cut(s) 244, 531
Eco130I CCWWGG 1 cut(s) 369
Eco47I GGWCC 2 cut(s) 224, 606
Eco57I CTGAAG 2 cut(s) 309, 645
EcoO109I RGGNCCY 1 cut(s) 8
EcoRII CCWGG 1 cut(s) 211
EcoT14I CCWWGG 1 cut(s) 369
ErhI CCWWGG 1 cut(s) 369
FaeI CATG 5 cut(s) 65, 413, 575, 602, 695
FaiI YATR 9 cut(s) 63, 93, 198, 396, 411, 573, 600, 671, 693
FatI CATG 5 cut(s) 61, 409, 571, 598, 691
Fnu4HI GCNGC 7 cut(s) 19, 159, 162, 294, 375, 414, 636
FokI GGATG 1 cut(s) 414
Fsp4HI GCNGC 7 cut(s) 19, 159, 162, 294, 375, 414, 636
GluI GCNGC 7 cut(s) 19, 159, 162, 294, 375, 414, 636
GsaI CCCAGC 2 cut(s) 47, 168
GsuI CTGGAG 1 cut(s) 15
HaeIII GGCC 3 cut(s) 9, 60, 635
HapII CCGG 1 cut(s) 57
Hin1I GRCGYC 1 cut(s) 519
Hin1II CATG 5 cut(s) 65, 413, 575, 602, 695
HindIII AAGCTT 3 cut(s) 71, 116, 312
HinfI GANTC 2 cut(s) 86, 110
HpaII CCGG 1 cut(s) 57
HphI GGTGA 1 cut(s) 227
Hpy166II GTNNAC 2 cut(s) 448, 606
Hpy188I TCNGA 2 cut(s) 289, 504
Hpy188III TCNNGA 4 cut(s) 83, 107, 523, 665
Hpy8I GTNNAC 2 cut(s) 448, 606
Hpy99I CGWCG 1 cut(s) 524
HpyAV CCTTC 1 cut(s) 441
HpyCH4III ACNGT 2 cut(s) 445, 610
HpyCH4IV ACGT 1 cut(s) 519
HpyCH4V TGCA 6 cut(s) 158, 296, 338, 389, 551, 595
HpyF10VI GCNNNNNNNGC 5 cut(s) 15, 290, 344, 374, 656
HpyF3I CTNAG 3 cut(s) 492, 558, 651
HpySE526I ACGT 1 cut(s) 519
Hsp92I GRCGYC 1 cut(s) 519
Hsp92II CATG 5 cut(s) 65, 413, 575, 602, 695
KpnI GGTACC 1 cut(s) 185
Kzo9I GATC 4 cut(s) 276, 400, 499, 561
LmnI GCTCC 2 cut(s) 365, 488
Lsp1109I GCAGC 6 cut(s) 5, 148, 170, 280, 361, 400
LweI GCATC 3 cut(s) 193, 208, 293
MaeII ACGT 1 cut(s) 519
MalI GATC 4 cut(s) 278, 402, 501, 563
MboI GATC 4 cut(s) 276, 400, 499, 561
MboII GAAGA 6 cut(s) 119, 231, 395, 548, 589, 638
MfeI CAATTG 1 cut(s) 339
MluCI AATT 2 cut(s) 339, 530
MlyI GAGTC 2 cut(s) 80, 104
MmeI TCCRAC 1 cut(s) 211
MnlI CCTC 6 cut(s) 39, 532, 606, 625, 646, 649
MroXI GAANNNNTTC 1 cut(s) 238
MseI TTAA 6 cut(s) 39, 260, 317, 465, 545, 642
MslI CAYNNNNRTG 1 cut(s) 443
MspA1I CMGCKG 2 cut(s) 161, 638
MspI CCGG 1 cut(s) 57
MspR9I CCNGG 1 cut(s) 213
MunI CAATTG 1 cut(s) 339
MvaI CCWGG 1 cut(s) 213
MwoI GCNNNNNNNGC 5 cut(s) 15, 290, 344, 374, 656
NdeII GATC 4 cut(s) 276, 400, 499, 561
NlaIII CATG 5 cut(s) 65, 413, 575, 602, 695
NlaIV GGNNCC 4 cut(s) 55, 183, 367, 471
PdmI GAANNNNTTC 1 cut(s) 238
PflMI CCANNNNNTGG 1 cut(s) 50
PkrI GCNGC 7 cut(s) 20, 160, 163, 295, 376, 415, 637
PleI GAGTC 2 cut(s) 80, 104
PpsI GAGTC 2 cut(s) 80, 104
Psp6I CCWGG 1 cut(s) 211
PspFI CCCAGC 2 cut(s) 43, 164
PspGI CCWGG 1 cut(s) 211
PspN4I GGNNCC 4 cut(s) 55, 183, 367, 471
PspPI GGNCC 3 cut(s) 8, 224, 606
PstNI CAGNNNCTG 2 cut(s) 11, 164
PvuII CAGCTG 1 cut(s) 161
RsaI GTAC 3 cut(s) 183, 483, 617
RsaNI GTAC 3 cut(s) 182, 482, 616
RseI CAYNNNNRTG 1 cut(s) 443
SaqAI TTAA 6 cut(s) 39, 260, 317, 465, 545, 642
SatI GCNGC 7 cut(s) 19, 159, 162, 294, 375, 414, 636
Sau3AI GATC 4 cut(s) 276, 400, 499, 561
Sau96I GGNCC 3 cut(s) 8, 224, 606
SchI GAGTC 2 cut(s) 80, 104
ScrFI CCNGG 1 cut(s) 213
SfaNI GCATC 3 cut(s) 193, 208, 293
SfcI CTRYAG 1 cut(s) 348
SinI GGWCC 2 cut(s) 224, 606
SmiMI CAYNNNNRTG 1 cut(s) 443
Sse9I AATT 2 cut(s) 339, 530
SsiI CCGC 3 cut(s) 377, 461, 636
StyD4I CCNGG 1 cut(s) 211
StyI CCWWGG 1 cut(s) 369
TaaI ACNGT 2 cut(s) 445, 610
TaiI ACGT 1 cut(s) 522
TasI AATT 2 cut(s) 339, 530
TatI WGTACW 1 cut(s) 481
TauI GCSGC 1 cut(s) 638
Tru1I TTAA 6 cut(s) 39, 260, 317, 465, 545, 642
Tru9I TTAA 6 cut(s) 39, 260, 317, 465, 545, 642
TscAI CASTG 2 cut(s) 103, 448
TseI GCWGC 6 cut(s) 18, 158, 161, 293, 374, 413
TspDTI ATGAA 2 cut(s) 423, 427
TspRI CASTG 2 cut(s) 103, 448
Van91I CCANNNNNTGG 1 cut(s) 50
VpaK11BI GGWCC 2 cut(s) 224, 606
XmnI GAANNNNTTC 1 cut(s) 238
ZraI GACGTC 1 cut(s) 520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.