hydrolase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 163 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 38 |
| AgsI | TTSAA | 2 cut(s) | 14, 22 |
| AoxI | GGCC | 1 cut(s) | 100 |
| ApoI | RAATTY | 1 cut(s) | 163 |
| AseI | ATTAAT | 1 cut(s) | 222 |
| AspS9I | GGNCC | 1 cut(s) | 101 |
| BanII | GRGCYC | 1 cut(s) | 44 |
| BccI | CCATC | 1 cut(s) | 131 |
| BfaI | CTAG | 2 cut(s) | 68, 131 |
| BmgT120I | GGNCC | 1 cut(s) | 101 |
| BmrI | ACTGGG | 1 cut(s) | 47 |
| BmuI | ACTGGG | 1 cut(s) | 47 |
| BpmI | CTGGAG | 1 cut(s) | 72 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 38 |
| Bse1I | ACTGG | 2 cut(s) | 42, 103 |
| BseGI | GGATG | 1 cut(s) | 123 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 38 |
| BseMII | CTCAG | 1 cut(s) | 150 |
| BseNI | ACTGG | 2 cut(s) | 42, 103 |
| BshFI | GGCC | 1 cut(s) | 102 |
| BslFI | GGGAC | 2 cut(s) | 73, 93 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 38 |
| BsmFI | GGGAC | 2 cut(s) | 73, 93 |
| BsmI | GAATGC | 1 cut(s) | 20 |
| BsnI | GGCC | 1 cut(s) | 102 |
| Bsp1286I | GDGCHC | 1 cut(s) | 44 |
| BspANI | GGCC | 1 cut(s) | 102 |
| BspCNI | CTCAG | 1 cut(s) | 149 |
| BsrI | ACTGG | 2 cut(s) | 42, 103 |
| Bst6I | CTCTTC | 1 cut(s) | 186 |
| BstC8I | GCNNGC | 1 cut(s) | 120 |
| BstDEI | CTNAG | 1 cut(s) | 136 |
| BstF5I | GGATG | 1 cut(s) | 123 |
| BstNSI | RCATGY | 1 cut(s) | 122 |
| BsuRI | GGCC | 1 cut(s) | 102 |
| BtsCI | GGATG | 1 cut(s) | 123 |
| BtsIMutI | CAGTG | 2 cut(s) | 35, 96 |
| Cac8I | GCNNGC | 1 cut(s) | 120 |
| Cfr13I | GGNCC | 1 cut(s) | 101 |
| CviAII | CATG | 1 cut(s) | 119 |
| CviJI | RGCY | 3 cut(s) | 42, 102, 135 |
| CviKI_1 | RGCY | 3 cut(s) | 42, 102, 135 |
| DdeI | CTNAG | 1 cut(s) | 136 |
| Eam1104I | CTCTTC | 1 cut(s) | 186 |
| EarI | CTCTTC | 1 cut(s) | 186 |
| Eco24I | GRGCYC | 1 cut(s) | 44 |
| EcoT38I | GRGCYC | 1 cut(s) | 44 |
| FaeI | CATG | 1 cut(s) | 122 |
| FaiI | YATR | 4 cut(s) | 120, 153, 174, 195 |
| FaqI | GGGAC | 2 cut(s) | 73, 93 |
| FatI | CATG | 1 cut(s) | 118 |
| FokI | GGATG | 1 cut(s) | 110 |
| FriOI | GRGCYC | 1 cut(s) | 44 |
| FspBI | CTAG | 2 cut(s) | 68, 131 |
| GsuI | CTGGAG | 1 cut(s) | 72 |
| HaeIII | GGCC | 1 cut(s) | 102 |
| Hin1II | CATG | 1 cut(s) | 122 |
| HinfI | GANTC | 2 cut(s) | 61, 110 |
| Hpy188I | TCNGA | 1 cut(s) | 139 |
| Hpy188III | TCNNGA | 2 cut(s) | 22, 51 |
| HpyAV | CCTTC | 1 cut(s) | 137 |
| HpyF3I | CTNAG | 1 cut(s) | 136 |
| Hsp92II | CATG | 1 cut(s) | 122 |
| LmnI | GCTCC | 1 cut(s) | 53 |
| LpnPI | CCDG | 6 cut(s) | 23, 36, 42, 45, 84, 184 |
| MaeI | CTAG | 2 cut(s) | 68, 131 |
| MboII | GAAGA | 1 cut(s) | 173 |
| MhlI | GDGCHC | 1 cut(s) | 44 |
| MluCI | AATT | 2 cut(s) | 163, 190 |
| MlyI | GAGTC | 1 cut(s) | 55 |
| MseI | TTAA | 3 cut(s) | 84, 167, 222 |
| Mva1269I | GAATGC | 1 cut(s) | 20 |
| NlaIII | CATG | 1 cut(s) | 122 |
| NspI | RCATGY | 1 cut(s) | 122 |
| PaeI | GCATGC | 1 cut(s) | 122 |
| PctI | GAATGC | 1 cut(s) | 20 |
| PfeI | GAWTC | 1 cut(s) | 110 |
| PleI | GAGTC | 1 cut(s) | 55 |
| PpsI | GAGTC | 1 cut(s) | 55 |
| PshBI | ATTAAT | 1 cut(s) | 222 |
| PspPI | GGNCC | 1 cut(s) | 101 |
| SaqAI | TTAA | 3 cut(s) | 84, 167, 222 |
| Sau96I | GGNCC | 1 cut(s) | 101 |
| SchI | GAGTC | 1 cut(s) | 55 |
| SduI | GDGCHC | 1 cut(s) | 44 |
| SetI | ASST | 3 cut(s) | 92, 162, 173 |
| SphI | GCATGC | 1 cut(s) | 122 |
| Sse9I | AATT | 2 cut(s) | 163, 190 |
| SspMI | CTAG | 2 cut(s) | 68, 131 |
| TasI | AATT | 2 cut(s) | 163, 190 |
| TfiI | GAWTC | 1 cut(s) | 110 |
| Tru1I | TTAA | 3 cut(s) | 84, 167, 222 |
| Tru9I | TTAA | 3 cut(s) | 84, 167, 222 |
| TscAI | CASTG | 2 cut(s) | 42, 103 |
| TspDTI | ATGAA | 1 cut(s) | 168 |
| TspRI | CASTG | 2 cut(s) | 42, 103 |
| VspI | ATTAAT | 1 cut(s) | 222 |
| XapI | RAATTY | 1 cut(s) | 163 |
| XceI | RCATGY | 1 cut(s) | 122 |
| XspI | CTAG | 2 cut(s) | 68, 131 |