Rh4BG214600

1,4-beta-D-glucanase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000177
Physical Location & Seq
Reverse (-)
37375025 .. 37390316
15292 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG214600.1

Sequence Viewer

Length: 729 bp
ATGTCAGGCCCTCAGTGCTGCGCGAACCCACCGACCCTGAACCCAACCAGTGGATCCGGCCACGTTGAGAAGCTTGGTGGTCTCGACTCCTATCTCACTGGCTCTCCTGACTCCAAGCTTGCCATTGTTCTTGTCTCCGACATTTATGGGTATGAGGCTCCAAACTTGAGGAAGCTCGCAGACAAAGTTGCAGCTGCTGGCTTTTTTGTTGTGGTCCCTGACTTCTTTTATGGAGACCCTTATGTACTTCCAGCTGATGGATCCCATGGCGGTCTACCTACATGGCTGAAAGGTCATGGACCGGAGAAGGGATTTGAAGATGTTAAACTAGTTATTGGAGCTCTAAAAAGTAAAGGCGTCTCTGCAATAGGTGCTGCAGGCTTCTCTTTGGGAGCCAAGGTTGTAGTTGAACTTGCAAAGGGTGACTTTATCCAAGCTGCTGTTCTGGCTCATCCTTCTTCATTCACTGTGGATGATATCAAGGATGTCAACTTCTGCAGCTTGCTTCGAGAACCCACCAACACTAAGCTCAACGTACGGAAAAGGAACTGTCCTAGAATTTGGGGGCCTGAAGACTTACGATATGAAAACCCAAATATAAGTAACAACAACCTGTGTATAATAACAAAAGGGACTTATCTCCACCAACATCCAGAGATCCAAGATTCCAAGGAAACAACCATAGAAGTTAGGAACAGTTGTGTCAATCCGTCCCGGATTGAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
ko00361 ko00364 ko00623 ko01100 ko01110 ko01120 ko01130 map00361 map00364 map00623 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
DLH

Protein Analysis

242

Amino Acids

26.02

Weight (kDa)

6.59

Isoelectric Point (pI)

28.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DLH PF01738 29 - 156 6.4e-14 Dienelactone hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000177)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23570 AT3G23570 AT3G23570 AT3G23600 AT3G23600
fragaria_vesca FvH4_2g23960 FvH4_2g23960 FvH4_2g23960 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14330 FvH4_6g28031 FvH4_6g29270
malus_domestica MD13G1181200.v1.1 MD13G1181300.v1.1 MD13G1181400.v1.1 MD13G1181500.v1.1 MD13G1181600.v1.1 MD13G1181700.v1.1 MD13G1181800.v1.1 MD13G1181900.v1.1 MD15G1403300.v1.1 MD15G1403400.v1.1 MD16G1181900.v1.1 MD16G1182000.v1.1 MD16G1182200.v1.1 MD16G1182300.v1.1 MD16G1182400.v1.1
prunus_persica Prupe.1G149400_v2.0.a1 Prupe.1G149600_v2.0.a1 Prupe.1G149700_v2.0.a1 Prupe.1G149800_v2.0.a1 Prupe.1G549400_v2.0.a1 Prupe.8G245600_v2.0.a1
pyrus_communis pycom13g15640 pycom13g15650 pycom13g15670 pycom13g15700 pycom15g36070 pycom16g15270 pycom16g15300 pycom16g15310
rosa_chinensis RchiOBHm_Chr1g0334461 RchiOBHm_Chr1g0334471 RchiOBHm_Chr2g0131731 RchiOBHm_Chr4g0417881 RchiOBHm_Chr4g0418561 RchiOBHm_Chr4g0418571 RchiOBHm_Chr4g0418581 RchiOBHm_Chr4g0418591 RchiOBHm_Chr4g0418601 RchiOBHm_Chr4g0418621 RchiOBHm_Chr4g0418651 RchiOBHm_Chr4g0418661 RchiOBHm_Chr4g0418671 RchiOBHm_Chr4g0418681 RchiOBHm_Chr4g0418691 RchiOBHm_Chr4g0418701 RchiOBHm_Chr4g0421371 RchiOBHm_Chr6g0291601 RchiOBHm_Chr6g0291611 RchiOBHm_Chr6g0291621 RchiOBHm_Chr6g0291631 RchiOBHm_Chr7g0229821
rosa_laevigata RLG00000007843 RLG00000007844 RLG00000007846 RLG00000007847 RLG00000007849 RLG00000012101 RLG00000012104 RLG00000029451
rosa_multiflora Rmu_sc0000363.1_g000035 Rmu_sc0000935.1_g000003 Rmu_sc0000935.1_g000010 Rmu_sc0002226.1_g000008 Rmu_sc0002226.1_g000014 Rmu_sc0002589.1_g000031 Rmu_sc0003443.1_g000009 Rmu_sc0006431.1_g000001 Rmu_sc0006431.1_g000006 Rmu_sc0006431.1_g000023 Rmu_sc0006705.1_g000014 Rmu_sc0006705.1_g000015 Rmu_sc0006705.1_g000016 Rmu_sc0006705.1_g000017 Rmu_sc0006705.1_g000018 Rmu_sc0014797.1_g000001 Rmu_sc0041885.1_g000001
rosa_roxburghii Rroxscaffold_4G00316820 Rroxscaffold_5G00361830 Rroxscaffold_5G00361840 Rroxscaffold_5G00361850 Rroxscaffold_5G00361860 Rroxscaffold_5G00361880 Rroxscaffold_5G00361890 Rroxscaffold_5G00361900 Rroxscaffold_5G00361910 Rroxscaffold_5G00361920 Rroxscaffold_5G00361930 Rroxscaffold_7G00175910 Rroxscaffold_7G00175930 Rroxscaffold_7G00175940
rosa_rugosa Rorug02G0299300 Rorug04G0154500 Rorug04G0154600 Rorug04G0154700 Rorug06G0220500
rosa_samantha Rh1AG006300 Rh1AG139200 Rh1AG139300 Rh1BG105600 Rh1CG131200 Rh1CG131300 Rh1DG143200 Rh2AG210000 Rh2CG337500 Rh2DG216200 Rh2DG376200 Rh4AG215900 Rh4AG216000 Rh4AG216100 Rh4AG216200 Rh4AG216300 Rh4AG216400 Rh4BG214300 Rh4BG214400 Rh4BG214500 Rh4BG214600 Rh4BG214700 Rh4BG214800 Rh4CG212300 Rh4CG227500 Rh4CG227700 Rh4CG227900 Rh4CG228000 Rh4CG228100 Rh4DG214200 Rh4DG215000 Rh4DG215200 Rh5AG059400 Rh6AG332900 Rh6AG333000 Rh6AG333100 Rh6BG339700 Rh6BG339900 Rh6CG346100 Rh6CG346200 Rh6DG333300 Rh6DG333400 Rh7AG435400 Rh7CG454500
rosa_wichuraiana Rw1G011480 Rw2G028580 Rw4G018270 Rw4G018280 Rw4G018290 Rw4G018360 Rw4G018390 Rw4G018400 Rw4G018410 Rw4G018420 Rw6G028910 Rw6G029000 Rw6G029010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 50, 257
AccI GTMKAC 1 cut(s) 274
AccII CGCG 1 cut(s) 23
AciI CCGC 1 cut(s) 270
AclWI GGATC 5 cut(s) 48, 61, 255, 268, 652
AcoI YGGCCR 1 cut(s) 58
AcsI RAATTY 1 cut(s) 558
AcuI CTGAAG 1 cut(s) 591
AcyI GRCGYC 1 cut(s) 357
AfaI GTAC 2 cut(s) 246, 537
AfiI CCNNNNNNNGG 3 cut(s) 50, 257, 308
AgsI TTSAA 3 cut(s) 317, 410, 722
AhlI ACTAGT 1 cut(s) 328
AjuI GAANNNNNNNTTGG 2 cut(s) 426, 458
AluBI AGCT 9 cut(s) 73, 118, 175, 194, 254, 341, 437, 501, 529
AluI AGCT 9 cut(s) 73, 118, 175, 194, 254, 341, 437, 501, 529
Alw21I GWGCWC 1 cut(s) 343
Alw26I GTCTC 4 cut(s) 86, 139, 228, 364
AlwI GGATC 5 cut(s) 48, 61, 255, 268, 652
AlwNI CAGNNNCTG 1 cut(s) 197
AoxI GGCC 3 cut(s) 7, 58, 566
ApeKI GCWGC 6 cut(s) 18, 191, 194, 374, 437, 498
ApoI RAATTY 1 cut(s) 558
AspLEI GCGC 1 cut(s) 23
AspS9I GGNCC 4 cut(s) 8, 214, 299, 566
AsuC2I CCSGG 1 cut(s) 715
AsuHPI GGTGA 1 cut(s) 434
AvaII GGWCC 2 cut(s) 214, 299
BamHI GGATCC 2 cut(s) 53, 260
BanII GRGCYC 1 cut(s) 343
BbsI GAAGAC 1 cut(s) 579
Bbv12I GWGCWC 1 cut(s) 343
BbvI GCAGC 6 cut(s) 5, 181, 203, 361, 424, 510
BccI CCATC 1 cut(s) 251
BcnI CCSGG 1 cut(s) 715
BcoDI GTCTC 4 cut(s) 86, 139, 228, 364
BcuI ACTAGT 1 cut(s) 328
BfaI CTAG 2 cut(s) 329, 555
BfmI CTRYAG 2 cut(s) 375, 496
BisI GCNGC 6 cut(s) 19, 192, 195, 375, 438, 499
BlsI GCNGC 6 cut(s) 20, 193, 196, 376, 439, 500
Bme1390I CCNGG 1 cut(s) 715
Bme18I GGWCC 2 cut(s) 214, 299
BmgT120I GGNCC 4 cut(s) 8, 214, 299, 566
BmiI GGNNCC 6 cut(s) 55, 159, 216, 262, 394, 567
BmrFI CCNGG 1 cut(s) 715
BpiI GAAGAC 1 cut(s) 579
BpuEI CTTGAG 1 cut(s) 187
BpuMI CCSGG 1 cut(s) 715
BsaHI GRCGYC 1 cut(s) 357
BsaI GGTCTC 2 cut(s) 86, 228
BsaJI CCNNGG 3 cut(s) 265, 396, 669
BsaWI WCCGGW 1 cut(s) 301
BsaXI ACNNNNNCTCC 4 cut(s) 88, 118, 384, 414
Bsc4I CCNNNNNNNGG 3 cut(s) 50, 257, 308
Bse1I ACTGG 2 cut(s) 48, 103
BseDI CCNNGG 3 cut(s) 265, 396, 669
BseGI GGATG 4 cut(s) 451, 478, 490, 649
BseLI CCNNNNNNNGG 3 cut(s) 50, 257, 308
BseMII CTCAG 1 cut(s) 26
BseNI ACTGG 2 cut(s) 48, 103
BseXI GCAGC 6 cut(s) 5, 181, 203, 361, 424, 510
Bsh1236I CGCG 1 cut(s) 23
BshFI GGCC 3 cut(s) 9, 60, 568
BsiHKAI GWGCWC 1 cut(s) 343
BsiSI CCGG 3 cut(s) 57, 302, 715
BsiWI CGTACG 1 cut(s) 535
BslFI GGGAC 3 cut(s) 200, 646, 697
BslI CCNNNNNNNGG 3 cut(s) 50, 257, 308
BsmAI GTCTC 4 cut(s) 86, 139, 228, 364
BsmBI CGTCTC 1 cut(s) 364
BsmFI GGGAC 3 cut(s) 200, 646, 697
BsnI GGCC 3 cut(s) 9, 60, 568
Bso31I GGTCTC 2 cut(s) 86, 228
Bsp1286I GDGCHC 1 cut(s) 343
Bsp143I GATC 3 cut(s) 53, 260, 657
Bsp19I CCATGG 1 cut(s) 265
BspACI CCGC 1 cut(s) 270
BspANI GGCC 3 cut(s) 9, 60, 568
BspCNI CTCAG 1 cut(s) 25
BspFNI CGCG 1 cut(s) 23
BspLI GGNNCC 6 cut(s) 55, 159, 216, 262, 394, 567
BspMAI CTGCAG 2 cut(s) 379, 500
BspPI GGATC 5 cut(s) 48, 61, 255, 268, 652
BspTNI GGTCTC 2 cut(s) 86, 228
BsrI ACTGG 2 cut(s) 48, 103
BssECI CCNNGG 3 cut(s) 265, 396, 669
BssMI GATC 3 cut(s) 53, 260, 657
BssNI GRCGYC 1 cut(s) 357
BssT1I CCWWGG 3 cut(s) 265, 396, 669
Bst4CI ACNGT 3 cut(s) 469, 551, 698
BstACI GRCGYC 1 cut(s) 357
BstAPI GCANNNNNTGC 1 cut(s) 371
BstC8I GCNNGC 5 cut(s) 120, 177, 199, 379, 503
BstDEI CTNAG 2 cut(s) 12, 525
BstDSI CCRYGG 1 cut(s) 265
BstF5I GGATG 4 cut(s) 451, 478, 490, 649
BstFNI CGCG 1 cut(s) 23
BstHHI GCGC 1 cut(s) 23
BstKTI GATC 3 cut(s) 56, 263, 660
BstMAI GTCTC 4 cut(s) 86, 139, 228, 364
BstMBI GATC 3 cut(s) 53, 260, 657
BstMWI GCNNNNNNNGC 3 cut(s) 15, 371, 446
BstSCI CCNGG 1 cut(s) 713
BstSFI CTRYAG 2 cut(s) 375, 496
BstUI CGCG 1 cut(s) 23
BstV1I GCAGC 6 cut(s) 5, 181, 203, 361, 424, 510
BstV2I GAAGAC 1 cut(s) 579
BstX2I RGATCY 3 cut(s) 53, 260, 657
BstYI RGATCY 3 cut(s) 53, 260, 657
BsuRI GGCC 3 cut(s) 9, 60, 568
BtgI CCRYGG 1 cut(s) 265
BtsCI GGATG 4 cut(s) 451, 478, 490, 649
BtsIMutI CAGTG 4 cut(s) 20, 55, 96, 465
Cac8I GCNNGC 5 cut(s) 120, 177, 199, 379, 503
CaiI CAGNNNCTG 1 cut(s) 197
CfoI GCGC 1 cut(s) 23
Cfr13I GGNCC 4 cut(s) 8, 214, 299, 566
CseI GACGC 1 cut(s) 346
Csp6I GTAC 2 cut(s) 245, 536
CviAII CATG 3 cut(s) 266, 282, 296
CviQI GTAC 2 cut(s) 245, 536
DdeI CTNAG 2 cut(s) 12, 525
DpnI GATC 3 cut(s) 55, 262, 659
DpnII GATC 3 cut(s) 53, 260, 657
EaeI YGGCCR 1 cut(s) 58
Ecl136II GAGCTC 1 cut(s) 341
Eco130I CCWWGG 3 cut(s) 265, 396, 669
Eco24I GRGCYC 1 cut(s) 343
Eco31I GGTCTC 2 cut(s) 86, 228
Eco32I GATATC 1 cut(s) 478
Eco47I GGWCC 2 cut(s) 214, 299
Eco53kI GAGCTC 1 cut(s) 341
Eco57I CTGAAG 1 cut(s) 591
EcoICRI GAGCTC 1 cut(s) 341
EcoO109I RGGNCCY 2 cut(s) 8, 566
EcoRV GATATC 1 cut(s) 478
EcoT14I CCWWGG 3 cut(s) 265, 396, 669
EcoT38I GRGCYC 1 cut(s) 343
ErhI CCWWGG 3 cut(s) 265, 396, 669
Esp3I CGTCTC 1 cut(s) 364
FaeI CATG 3 cut(s) 269, 285, 299
FalI AAGNNNNNCTT 2 cut(s) 410, 442
FaqI GGGAC 3 cut(s) 200, 646, 697
FatI CATG 3 cut(s) 265, 281, 295
FblI GTMKAC 1 cut(s) 274
Fnu4HI GCNGC 6 cut(s) 19, 192, 195, 375, 438, 499
FokI GGATG 4 cut(s) 438, 485, 497, 636
FriOI GRGCYC 1 cut(s) 343
Fsp4HI GCNGC 6 cut(s) 19, 192, 195, 375, 438, 499
FspBI CTAG 2 cut(s) 329, 555
GlaI GCGC 1 cut(s) 22
GluI GCNGC 6 cut(s) 19, 192, 195, 375, 438, 499
HaeIII GGCC 3 cut(s) 9, 60, 568
HapII CCGG 3 cut(s) 57, 302, 715
HgaI GACGC 1 cut(s) 346
HhaI GCGC 1 cut(s) 23
Hin1I GRCGYC 1 cut(s) 357
Hin1II CATG 3 cut(s) 269, 285, 299
Hin6I GCGC 1 cut(s) 21
HinP1I GCGC 1 cut(s) 21
HincII GTYRAC 1 cut(s) 490
HindII GTYRAC 1 cut(s) 490
HindIII AAGCTT 2 cut(s) 71, 116
HinfI GANTC 3 cut(s) 86, 110, 665
HpaII CCGG 3 cut(s) 57, 302, 715
HphI GGTGA 1 cut(s) 434
Hpy166II GTNNAC 2 cut(s) 275, 490
Hpy188I TCNGA 1 cut(s) 139
Hpy188III TCNNGA 4 cut(s) 83, 107, 509, 653
Hpy8I GTNNAC 2 cut(s) 275, 490
HpyAV CCTTC 2 cut(s) 301, 465
HpyCH4III ACNGT 3 cut(s) 469, 551, 698
HpyCH4IV ACGT 2 cut(s) 63, 534
HpyCH4V TGCA 5 cut(s) 191, 365, 377, 416, 498
HpyF10VI GCNNNNNNNGC 3 cut(s) 15, 371, 446
HpyF3I CTNAG 2 cut(s) 12, 525
HpySE526I ACGT 2 cut(s) 63, 534
Hsp92I GRCGYC 1 cut(s) 357
Hsp92II CATG 3 cut(s) 269, 285, 299
HspAI GCGC 1 cut(s) 21
Kzo9I GATC 3 cut(s) 53, 260, 657
LmnI GCTCC 3 cut(s) 163, 338, 392
Lsp1109I GCAGC 6 cut(s) 5, 181, 203, 361, 424, 510
MaeI CTAG 2 cut(s) 329, 555
MaeII ACGT 2 cut(s) 63, 534
MaeIII GTNAC 2 cut(s) 422, 602
MalI GATC 3 cut(s) 55, 262, 659
MboI GATC 3 cut(s) 53, 260, 657
MboII GAAGA 3 cut(s) 329, 450, 584
MflI RGATCY 3 cut(s) 53, 260, 657
MhlI GDGCHC 1 cut(s) 343
MluCI AATT 1 cut(s) 558
MlyI GAGTC 2 cut(s) 80, 104
MmeI TCCRAC 1 cut(s) 162
MnlI CCTC 3 cut(s) 21, 148, 162
MseI TTAA 1 cut(s) 324
MspA1I CMGCKG 2 cut(s) 194, 254
MspI CCGG 3 cut(s) 57, 302, 715
MspR9I CCNGG 1 cut(s) 715
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 3 cut(s) 15, 371, 446
NciI CCSGG 1 cut(s) 715
NcoI CCATGG 1 cut(s) 265
NdeII GATC 3 cut(s) 53, 260, 657
NlaIII CATG 3 cut(s) 269, 285, 299
NlaIV GGNNCC 6 cut(s) 55, 159, 216, 262, 394, 567
NmuCI GTSAC 1 cut(s) 422
PfeI GAWTC 1 cut(s) 665
Pfl23II CGTACG 1 cut(s) 535
PflMI CCANNNNNTGG 2 cut(s) 50, 257
PfoI TCCNGGA 1 cut(s) 713
PkrI GCNGC 6 cut(s) 20, 193, 196, 376, 439, 500
PleI GAGTC 2 cut(s) 80, 104
PpsI GAGTC 2 cut(s) 80, 104
Psp124BI GAGCTC 1 cut(s) 343
PspLI CGTACG 1 cut(s) 535
PspN4I GGNNCC 6 cut(s) 55, 159, 216, 262, 394, 567
PspPI GGNCC 4 cut(s) 8, 214, 299, 566
PstI CTGCAG 2 cut(s) 379, 500
PstNI CAGNNNCTG 1 cut(s) 197
PsuI RGATCY 3 cut(s) 53, 260, 657
PvuII CAGCTG 2 cut(s) 194, 254
RsaI GTAC 2 cut(s) 246, 537
RsaNI GTAC 2 cut(s) 245, 536
SacI GAGCTC 1 cut(s) 343
SaqAI TTAA 1 cut(s) 324
SatI GCNGC 6 cut(s) 19, 192, 195, 375, 438, 499
Sau3AI GATC 3 cut(s) 53, 260, 657
Sau96I GGNCC 4 cut(s) 8, 214, 299, 566
SchI GAGTC 2 cut(s) 80, 104
ScrFI CCNGG 1 cut(s) 715
SduI GDGCHC 1 cut(s) 343
SfcI CTRYAG 2 cut(s) 375, 496
SinI GGWCC 2 cut(s) 214, 299
SmlI CTYRAG 1 cut(s) 166
SmoI CTYRAG 1 cut(s) 166
SpeI ACTAGT 1 cut(s) 328
Sse9I AATT 1 cut(s) 558
SsiI CCGC 1 cut(s) 270
SspMI CTAG 2 cut(s) 329, 555
SstI GAGCTC 1 cut(s) 343
StyD4I CCNGG 1 cut(s) 713
StyI CCWWGG 3 cut(s) 265, 396, 669
TaaI ACNGT 3 cut(s) 469, 551, 698
TaiI ACGT 2 cut(s) 66, 537
TaqI TCGA 2 cut(s) 84, 508
TasI AATT 1 cut(s) 558
TatI WGTACW 1 cut(s) 244
TfiI GAWTC 1 cut(s) 665
Tru1I TTAA 1 cut(s) 324
Tru9I TTAA 1 cut(s) 324
TscAI CASTG 4 cut(s) 20, 55, 103, 472
TseFI GTSAC 1 cut(s) 422
TseI GCWGC 6 cut(s) 18, 191, 194, 374, 437, 498
Tsp45I GTSAC 1 cut(s) 422
TspDTI ATGAA 2 cut(s) 450, 600
TspGWI ACGGA 2 cut(s) 553, 699
TspRI CASTG 4 cut(s) 20, 55, 103, 472
Van91I CCANNNNNTGG 2 cut(s) 50, 257
VpaK11BI GGWCC 2 cut(s) 214, 299
XapI RAATTY 1 cut(s) 558
XmiI GTMKAC 1 cut(s) 274
XspI CTAG 2 cut(s) 329, 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.