MD16G1181900.v1.1

1,4-beta-D-glucanase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000177
Physical Location & Seq
Reverse (-)
15603320 .. 15608032
4713 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1181900.v1.1.491

Sequence Viewer

Length: 828 bp
ATGCCCTATGGGTTAATGAGAATTAAAAGCCTTGTGAAGATTTTCCCGGAAGTGGAACCGGAGGTAAGACGTGATGATGAAGCAGATTGTAAGGAAAAAAAGAAGAAAGAAATCATGGCAAGCTCTGAGTGCTGCTCAAACCCACCAGCCCTGAACCCGTCCAGCGGCTCCGGCCACGTCGAAAAGCTCGGCGGTTTCGATTCCTATGTCACTGGCTCCCCTGACTCTAAGCTTGCCGTTCTTCTCGTCCATGACGCTTTTGGATATGAAGCTCCAAAGGCTAGGATGCTTGCAGACAAAATTGCAGCTGCTGGGTTCATTGTTGTGCTCCCAGACTTCTTCAATCGAGACCCTTTTAATGGGGACCTTGCCTCTATACCGGTTTGGATAAAAGCTCACGCACCGGACAAAACAATCGAAGATGCAAAACTGGTAATTGAAGCTTTAAAAAGTCAAGGTGTTTCTGCAATTGGTGCTGTAGGCTTCTGTTGGGGCGGCAAGGGTGTCGTTGAACTTGCGAAGCATGACTTTATCCAAGCCGCGGTTCTGTGTCATCCTTCATTTGTCACTCTGGATGATATCAAGGCGGTTAAGGTTCCGATTTCTGTACTTGGAGCTGAGATTGATCAGCTGACTCCACCTGAAGTCGTCAAACAATTTGAAGAGGTTTTAACTGCAAAACCTGAGATTAAAAGCCATGTGAAGATATTCCCGAAGATCGCACACGGGTGGACGTTGAGGTACAACGATGAAGACGAGGCGGCTGTCAAGGCAGCTGAGGAGGCTCATCAAGACTTGTTGGGGTGGTTCTTGGAGCATATCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
ko00361 ko00364 ko00623 ko01100 ko01110 ko01120 ko01130 map00361 map00364 map00623 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
DLH

Protein Analysis

276

Amino Acids

30.02

Weight (kDa)

5.52

Isoelectric Point (pI)

35.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DLH PF01738 66 - 272 7.2e-32 Dienelactone hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000177)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23570 AT3G23570 AT3G23570 AT3G23600 AT3G23600
fragaria_vesca FvH4_2g23960 FvH4_2g23960 FvH4_2g23960 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14330 FvH4_6g28031 FvH4_6g29270
malus_domestica MD13G1181200.v1.1 MD13G1181300.v1.1 MD13G1181400.v1.1 MD13G1181500.v1.1 MD13G1181600.v1.1 MD13G1181700.v1.1 MD13G1181800.v1.1 MD13G1181900.v1.1 MD15G1403300.v1.1 MD15G1403400.v1.1 MD16G1181900.v1.1 MD16G1182000.v1.1 MD16G1182200.v1.1 MD16G1182300.v1.1 MD16G1182400.v1.1
prunus_persica Prupe.1G149400_v2.0.a1 Prupe.1G149600_v2.0.a1 Prupe.1G149700_v2.0.a1 Prupe.1G149800_v2.0.a1 Prupe.1G549400_v2.0.a1 Prupe.8G245600_v2.0.a1
pyrus_communis pycom13g15640 pycom13g15650 pycom13g15670 pycom13g15700 pycom15g36070 pycom16g15270 pycom16g15300 pycom16g15310
rosa_chinensis RchiOBHm_Chr1g0334461 RchiOBHm_Chr1g0334471 RchiOBHm_Chr2g0131731 RchiOBHm_Chr4g0417881 RchiOBHm_Chr4g0418561 RchiOBHm_Chr4g0418571 RchiOBHm_Chr4g0418581 RchiOBHm_Chr4g0418591 RchiOBHm_Chr4g0418601 RchiOBHm_Chr4g0418621 RchiOBHm_Chr4g0418651 RchiOBHm_Chr4g0418661 RchiOBHm_Chr4g0418671 RchiOBHm_Chr4g0418681 RchiOBHm_Chr4g0418691 RchiOBHm_Chr4g0418701 RchiOBHm_Chr4g0421371 RchiOBHm_Chr6g0291601 RchiOBHm_Chr6g0291611 RchiOBHm_Chr6g0291621 RchiOBHm_Chr6g0291631 RchiOBHm_Chr7g0229821
rosa_laevigata RLG00000007843 RLG00000007844 RLG00000007846 RLG00000007847 RLG00000007849 RLG00000012101 RLG00000012104 RLG00000029451
rosa_multiflora Rmu_sc0000363.1_g000035 Rmu_sc0000935.1_g000003 Rmu_sc0000935.1_g000010 Rmu_sc0002226.1_g000008 Rmu_sc0002226.1_g000014 Rmu_sc0002589.1_g000031 Rmu_sc0003443.1_g000009 Rmu_sc0006431.1_g000001 Rmu_sc0006431.1_g000006 Rmu_sc0006431.1_g000023 Rmu_sc0006705.1_g000014 Rmu_sc0006705.1_g000015 Rmu_sc0006705.1_g000016 Rmu_sc0006705.1_g000017 Rmu_sc0006705.1_g000018 Rmu_sc0014797.1_g000001 Rmu_sc0041885.1_g000001
rosa_roxburghii Rroxscaffold_4G00316820 Rroxscaffold_5G00361830 Rroxscaffold_5G00361840 Rroxscaffold_5G00361850 Rroxscaffold_5G00361860 Rroxscaffold_5G00361880 Rroxscaffold_5G00361890 Rroxscaffold_5G00361900 Rroxscaffold_5G00361910 Rroxscaffold_5G00361920 Rroxscaffold_5G00361930 Rroxscaffold_7G00175910 Rroxscaffold_7G00175930 Rroxscaffold_7G00175940
rosa_rugosa Rorug02G0299300 Rorug04G0154500 Rorug04G0154600 Rorug04G0154700 Rorug06G0220500
rosa_samantha Rh1AG006300 Rh1AG139200 Rh1AG139300 Rh1BG105600 Rh1CG131200 Rh1CG131300 Rh1DG143200 Rh2AG210000 Rh2CG337500 Rh2DG216200 Rh2DG376200 Rh4AG215900 Rh4AG216000 Rh4AG216100 Rh4AG216200 Rh4AG216300 Rh4AG216400 Rh4BG214300 Rh4BG214400 Rh4BG214500 Rh4BG214600 Rh4BG214700 Rh4BG214800 Rh4CG212300 Rh4CG227500 Rh4CG227700 Rh4CG227900 Rh4CG228000 Rh4CG228100 Rh4DG214200 Rh4DG215000 Rh4DG215200 Rh5AG059400 Rh6AG332900 Rh6AG333000 Rh6AG333100 Rh6BG339700 Rh6BG339900 Rh6CG346100 Rh6CG346200 Rh6DG333300 Rh6DG333400 Rh7AG435400 Rh7CG454500
rosa_wichuraiana Rw1G011480 Rw2G028580 Rw4G018270 Rw4G018280 Rw4G018290 Rw4G018360 Rw4G018390 Rw4G018400 Rw4G018410 Rw4G018420 Rw6G028910 Rw6G029000 Rw6G029010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 542
AciI CCGC 7 cut(s) 165, 192, 495, 540, 542, 587, 761
AcoI YGGCCR 1 cut(s) 172
AcuI CTGAAG 1 cut(s) 663
AfaI GTAC 2 cut(s) 609, 743
AfiI CCNNNNNNNGG 4 cut(s) 52, 164, 359, 541
AgeI ACCGGT 1 cut(s) 379
AgsI TTSAA 4 cut(s) 343, 440, 512, 662
AjiI CACGTC 2 cut(s) 71, 178
AjuI GAANNNNNNNTTGG 2 cut(s) 528, 560
AleI CACNNNNGTG 1 cut(s) 727
Alw21I GWGCWC 1 cut(s) 330
Alw26I GTCTC 1 cut(s) 342
AlwNI CAGNNNCTG 1 cut(s) 311
AoxI GGCC 1 cut(s) 172
ApeKI GCWGC 4 cut(s) 132, 305, 308, 773
AsiGI ACCGGT 1 cut(s) 379
Asp700I GAANNNNTTC 2 cut(s) 41, 707
AspS9I GGNCC 1 cut(s) 364
AsuC2I CCSGG 1 cut(s) 47
AvaII GGWCC 1 cut(s) 364
BbsI GAAGAC 1 cut(s) 759
Bbv12I GWGCWC 1 cut(s) 330
BbvCI CCTCAGC 1 cut(s) 777
BbvI GCAGC 4 cut(s) 119, 295, 317, 785
BceAI ACGGC 1 cut(s) 221
BcgI CGANNNNNNTGC 2 cut(s) 487, 521
BclI TGATCA 1 cut(s) 625
BcnI CCSGG 1 cut(s) 47
BcoDI GTCTC 1 cut(s) 342
BfaI CTAG 1 cut(s) 282
BfmI CTRYAG 1 cut(s) 477
BisI GCNGC 8 cut(s) 133, 166, 306, 309, 496, 540, 762, 774
BlsI GCNGC 8 cut(s) 134, 167, 307, 310, 497, 541, 763, 775
Bme1390I CCNGG 1 cut(s) 47
Bme18I GGWCC 1 cut(s) 364
BmgBI CACGTC 2 cut(s) 71, 178
BmgT120I GGNCC 1 cut(s) 364
BmiI GGNNCC 5 cut(s) 57, 169, 217, 365, 597
BmrFI CCNGG 1 cut(s) 47
BmsI GCATC 2 cut(s) 276, 412
BpiI GAAGAC 1 cut(s) 759
BplI GAGNNNNNCTC 2 cut(s) 119, 151
Bpu10I CCTNAGC 1 cut(s) 777
BpuMI CCSGG 1 cut(s) 47
BsaI GGTCTC 1 cut(s) 342
BsaJI CCNNGG 1 cut(s) 540
BsaWI WCCGGW 3 cut(s) 58, 379, 403
Bsc4I CCNNNNNNNGG 4 cut(s) 52, 164, 359, 541
Bse118I RCCGGY 1 cut(s) 379
Bse1I ACTGG 2 cut(s) 217, 435
BseDI CCNNGG 1 cut(s) 540
BseGI GGATG 3 cut(s) 291, 553, 580
BseLI CCNNNNNNNGG 4 cut(s) 52, 164, 359, 541
BseMII CTCAG 4 cut(s) 117, 609, 675, 768
BseNI ACTGG 2 cut(s) 217, 435
BseRI GAGGAG 1 cut(s) 794
BseXI GCAGC 4 cut(s) 119, 295, 317, 785
BseYI CCCAGC 1 cut(s) 311
Bsh1236I CGCG 1 cut(s) 542
BshFI GGCC 1 cut(s) 174
BshTI ACCGGT 1 cut(s) 379
BsiHKAI GWGCWC 1 cut(s) 330
BsiSI CCGG 5 cut(s) 47, 59, 171, 380, 404
BslFI GGGAC 1 cut(s) 377
BslI CCNNNNNNNGG 4 cut(s) 52, 164, 359, 541
BsmAI GTCTC 1 cut(s) 342
BsmFI GGGAC 1 cut(s) 377
BsnI GGCC 1 cut(s) 174
Bso31I GGTCTC 1 cut(s) 342
Bsp1286I GDGCHC 1 cut(s) 330
Bsp143I GATC 2 cut(s) 625, 717
BspACI CCGC 7 cut(s) 165, 192, 495, 540, 542, 587, 761
BspANI GGCC 1 cut(s) 174
BspCNI CTCAG 4 cut(s) 118, 610, 676, 769
BspFNI CGCG 1 cut(s) 542
BspLI GGNNCC 5 cut(s) 57, 169, 217, 365, 597
BspTNI GGTCTC 1 cut(s) 342
BsrFI RCCGGY 1 cut(s) 379
BsrI ACTGG 2 cut(s) 217, 435
BssAI RCCGGY 1 cut(s) 379
BssECI CCNNGG 1 cut(s) 540
BssMI GATC 2 cut(s) 625, 717
Bst6I CTCTTC 1 cut(s) 657
BstAPI GCANNNNNTGC 1 cut(s) 473
BstC8I GCNNGC 3 cut(s) 121, 234, 291
BstDEI CTNAG 5 cut(s) 126, 228, 618, 684, 777
BstDSI CCRYGG 1 cut(s) 540
BstF5I GGATG 3 cut(s) 291, 553, 580
BstFNI CGCG 1 cut(s) 542
BstKTI GATC 2 cut(s) 628, 720
BstMAI GTCTC 1 cut(s) 342
BstMBI GATC 2 cut(s) 625, 717
BstMWI GCNNNNNNNGC 6 cut(s) 129, 171, 278, 473, 770, 782
BstSCI CCNGG 1 cut(s) 45
BstSFI CTRYAG 1 cut(s) 477
BstUI CGCG 1 cut(s) 542
BstV1I GCAGC 4 cut(s) 119, 295, 317, 785
BstV2I GAAGAC 1 cut(s) 759
BsuRI GGCC 1 cut(s) 174
BtgI CCRYGG 1 cut(s) 540
BtrI CACGTC 2 cut(s) 71, 178
BtsCI GGATG 3 cut(s) 291, 553, 580
BtsIMutI CAGTG 1 cut(s) 210
Cac8I GCNNGC 3 cut(s) 121, 234, 291
CaiI CAGNNNCTG 1 cut(s) 311
Cfr10I RCCGGY 1 cut(s) 379
Cfr13I GGNCC 1 cut(s) 364
Cfr42I CCGCGG 1 cut(s) 543
CseI GACGC 1 cut(s) 263
Csp6I GTAC 2 cut(s) 608, 742
CspAI ACCGGT 1 cut(s) 379
CviAII CATG 4 cut(s) 115, 251, 524, 698
CviQI GTAC 2 cut(s) 608, 742
DdeI CTNAG 5 cut(s) 126, 228, 618, 684, 777
DpnI GATC 2 cut(s) 627, 719
DpnII GATC 2 cut(s) 625, 717
DraI TTTAAA 1 cut(s) 447
EaeI YGGCCR 1 cut(s) 172
Eam1104I CTCTTC 1 cut(s) 657
EarI CTCTTC 1 cut(s) 657
Eco31I GGTCTC 1 cut(s) 342
Eco32I GATATC 1 cut(s) 580
Eco47I GGWCC 1 cut(s) 364
Eco57I CTGAAG 1 cut(s) 663
EcoO109I RGGNCCY 1 cut(s) 364
EcoRV GATATC 1 cut(s) 580
FaeI CATG 4 cut(s) 118, 254, 527, 701
FaiI YATR 9 cut(s) 9, 116, 207, 252, 267, 377, 525, 699, 819
FalI AAGNNNNNCTT 2 cut(s) 512, 544
FaqI GGGAC 1 cut(s) 377
FatI CATG 4 cut(s) 114, 250, 523, 697
FbaI TGATCA 1 cut(s) 625
Fnu4HI GCNGC 8 cut(s) 133, 166, 306, 309, 496, 540, 762, 774
FokI GGATG 3 cut(s) 298, 540, 587
Fsp4HI GCNGC 8 cut(s) 133, 166, 306, 309, 496, 540, 762, 774
FspBI CTAG 1 cut(s) 282
GluI GCNGC 8 cut(s) 133, 166, 306, 309, 496, 540, 762, 774
GsaI CCCAGC 1 cut(s) 315
HaeIII GGCC 1 cut(s) 174
HapII CCGG 5 cut(s) 47, 59, 171, 380, 404
HgaI GACGC 1 cut(s) 263
Hin1II CATG 4 cut(s) 118, 254, 527, 701
HindIII AAGCTT 2 cut(s) 230, 441
HinfI GANTC 3 cut(s) 200, 224, 634
HpaII CCGG 5 cut(s) 47, 59, 171, 380, 404
Hpy166II GTNNAC 1 cut(s) 732
Hpy188I TCNGA 2 cut(s) 127, 600
Hpy188III TCNNGA 4 cut(s) 347, 572, 712, 791
Hpy8I GTNNAC 1 cut(s) 732
Hpy99I CGWCG 1 cut(s) 182
HpyAV CCTTC 1 cut(s) 567
HpyCH4IV ACGT 3 cut(s) 70, 177, 734
HpyCH4V TGCA 5 cut(s) 293, 305, 425, 467, 677
HpyF10VI GCNNNNNNNGC 6 cut(s) 129, 171, 278, 473, 770, 782
HpyF3I CTNAG 5 cut(s) 126, 228, 618, 684, 777
HpySE526I ACGT 3 cut(s) 70, 177, 734
Hsp92II CATG 4 cut(s) 118, 254, 527, 701
Ksp22I TGATCA 1 cut(s) 625
KspI CCGCGG 1 cut(s) 543
Kzo9I GATC 2 cut(s) 625, 717
LmnI GCTCC 6 cut(s) 173, 221, 277, 333, 614, 814
Lsp1109I GCAGC 4 cut(s) 119, 295, 317, 785
LweI GCATC 2 cut(s) 276, 412
MaeI CTAG 1 cut(s) 282
MaeII ACGT 3 cut(s) 70, 177, 734
MaeIII GTNAC 2 cut(s) 208, 565
MalI GATC 2 cut(s) 627, 719
MboI GATC 2 cut(s) 625, 717
MboII GAAGA 9 cut(s) 49, 115, 233, 331, 431, 674, 715, 727, 764
MfeI CAATTG 1 cut(s) 468
MhlI GDGCHC 1 cut(s) 330
MluCI AATT 5 cut(s) 21, 300, 435, 468, 656
MlyI GAGTC 2 cut(s) 218, 628
MnlI CCTC 7 cut(s) 55, 382, 658, 732, 751, 772, 775
MroXI GAANNNNTTC 2 cut(s) 41, 707
MseI TTAA 7 cut(s) 14, 24, 357, 446, 591, 671, 690
MslI CAYNNNNRTG 2 cut(s) 323, 727
MspA1I CMGCKG 5 cut(s) 165, 308, 542, 631, 776
MspI CCGG 5 cut(s) 47, 59, 171, 380, 404
MspR9I CCNGG 1 cut(s) 47
MunI CAATTG 1 cut(s) 468
MvnI CGCG 1 cut(s) 542
MwoI GCNNNNNNNGC 6 cut(s) 129, 171, 278, 473, 770, 782
NciI CCSGG 1 cut(s) 47
NdeII GATC 2 cut(s) 625, 717
NlaIII CATG 4 cut(s) 118, 254, 527, 701
NlaIV GGNNCC 5 cut(s) 57, 169, 217, 365, 597
NmeAIII GCCGAG 1 cut(s) 168
NmuCI GTSAC 2 cut(s) 208, 565
OliI CACNNNNGTG 1 cut(s) 727
PcsI WCGNNNNNNNCGW 2 cut(s) 195, 753
PdmI GAANNNNTTC 2 cut(s) 41, 707
PfeI GAWTC 1 cut(s) 200
PfoI TCCNGGA 1 cut(s) 45
PinAI ACCGGT 1 cut(s) 379
PkrI GCNGC 8 cut(s) 134, 167, 307, 310, 497, 541, 763, 775
PleI GAGTC 2 cut(s) 218, 628
PpsI GAGTC 2 cut(s) 218, 628
PpuMI RGGWCCY 1 cut(s) 364
Psp5II RGGWCCY 1 cut(s) 364
PspFI CCCAGC 1 cut(s) 311
PspN4I GGNNCC 5 cut(s) 57, 169, 217, 365, 597
PspPI GGNCC 1 cut(s) 364
PspPPI RGGWCCY 1 cut(s) 364
PstNI CAGNNNCTG 1 cut(s) 311
PvuII CAGCTG 3 cut(s) 308, 631, 776
RsaI GTAC 2 cut(s) 609, 743
RsaNI GTAC 2 cut(s) 608, 742
RseI CAYNNNNRTG 2 cut(s) 323, 727
SacII CCGCGG 1 cut(s) 543
SaqAI TTAA 7 cut(s) 14, 24, 357, 446, 591, 671, 690
SatI GCNGC 8 cut(s) 133, 166, 306, 309, 496, 540, 762, 774
Sau3AI GATC 2 cut(s) 625, 717
Sau96I GGNCC 1 cut(s) 364
SchI GAGTC 2 cut(s) 218, 628
ScrFI CCNGG 1 cut(s) 47
SduI GDGCHC 1 cut(s) 330
SfaNI GCATC 2 cut(s) 276, 412
SfcI CTRYAG 1 cut(s) 477
Sfr303I CCGCGG 1 cut(s) 543
SgrBI CCGCGG 1 cut(s) 543
SinI GGWCC 1 cut(s) 364
SmiMI CAYNNNNRTG 2 cut(s) 323, 727
Sse9I AATT 5 cut(s) 21, 300, 435, 468, 656
SsiI CCGC 7 cut(s) 165, 192, 495, 540, 542, 587, 761
SspMI CTAG 1 cut(s) 282
StyD4I CCNGG 1 cut(s) 45
TaiI ACGT 3 cut(s) 73, 180, 737
TaqI TCGA 4 cut(s) 180, 198, 346, 417
TasI AATT 5 cut(s) 21, 300, 435, 468, 656
TatI WGTACW 1 cut(s) 607
TauI GCSGC 4 cut(s) 168, 498, 542, 764
TfiI GAWTC 1 cut(s) 200
Tru1I TTAA 7 cut(s) 14, 24, 357, 446, 591, 671, 690
Tru9I TTAA 7 cut(s) 14, 24, 357, 446, 591, 671, 690
TscAI CASTG 1 cut(s) 217
TseFI GTSAC 2 cut(s) 208, 565
TseI GCWGC 4 cut(s) 132, 305, 308, 773
Tsp45I GTSAC 2 cut(s) 208, 565
TspDTI ATGAA 5 cut(s) 93, 282, 307, 549, 765
TspRI CASTG 1 cut(s) 217
VpaK11BI GGWCC 1 cut(s) 364
XcmI CCANNNNNNNNNTGG 1 cut(s) 257
XmnI GAANNNNTTC 2 cut(s) 41, 707
XspI CTAG 1 cut(s) 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.