MD16G1182200.v1.1

1,4-beta-D-glucanase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000177
Physical Location & Seq
Reverse (-)
15624508 .. 15627382
2875 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1182200.v1.1.491

Sequence Viewer

Length: 720 bp
ATGGCAAGCTCTGAGTGCTGCTCAAACCCTCCGACCCTGAACCCCTCCAGCGGCACCGGCCATGTCGAAAGCCTCGGCGGTCTGGACTCCTATGTCACTGGCTCCCCTGATTCCAACCTTGCCGTTCTTCTCATCTCTGACGTTTATGGATTTGAAGCTCCAAACTTGAGGAAGCTTGCTGACAAAGTTGCAGCTGCTGGGTTCTTCGTTGTGGTTCCTGACTTCCTTAACAAAGACCCTTATGCACCTGAAGATGCCAACAGACCTATTTCTGTTTGGATAAAAGATCATGGACCGGACAAGGGATTTGAGGATGCAAAGCCAGTTCTTGAAGCTTTAAAAAGTAAAGGTGTTTCTGCAATCGGTGCTGCAGGCTTCTGTTGGGGAGCCAAGGTTGTGGTTGAACTTTCAAAGCACGCTTCAATTCAAGCTGCTGTTCTGTGTCATCCGTCATTAGTCACTGTGGATGATATCAAGGAGGTTAAGGTTCCTATTTCTATACTTGGAGCTGAGATTGATCGGATGTCTCCGCCTGAAGTCGTGAAACAGTTTGAAGAGGTTTTAACTGCAAAACCTGAGGTTAAGAGCCATGTGAAAATATTCCCGAAAGTGTCACATGGGTGGACGGTGAGGTACAGTGTTAAAGACGAAGCAGCTTGTAAATCTGCCGAGGAGGCTCATCAAGATTTGTTGAAGTGGTTCTTGAACCATGTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
ko00361 ko00364 ko00623 ko01100 ko01110 ko01120 ko01130 map00361 map00364 map00623 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
DLH

Protein Analysis

240

Amino Acids

25.7

Weight (kDa)

5.76

Isoelectric Point (pI)

33.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DLH PF01738 29 - 236 1.5e-30 Dienelactone hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000177)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23570 AT3G23570 AT3G23570 AT3G23600 AT3G23600
fragaria_vesca FvH4_2g23960 FvH4_2g23960 FvH4_2g23960 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14330 FvH4_6g28031 FvH4_6g29270
malus_domestica MD13G1181200.v1.1 MD13G1181300.v1.1 MD13G1181400.v1.1 MD13G1181500.v1.1 MD13G1181600.v1.1 MD13G1181700.v1.1 MD13G1181800.v1.1 MD13G1181900.v1.1 MD15G1403300.v1.1 MD15G1403400.v1.1 MD16G1181900.v1.1 MD16G1182000.v1.1 MD16G1182200.v1.1 MD16G1182300.v1.1 MD16G1182400.v1.1
prunus_persica Prupe.1G149400_v2.0.a1 Prupe.1G149600_v2.0.a1 Prupe.1G149700_v2.0.a1 Prupe.1G149800_v2.0.a1 Prupe.1G549400_v2.0.a1 Prupe.8G245600_v2.0.a1
pyrus_communis pycom13g15640 pycom13g15650 pycom13g15670 pycom13g15700 pycom15g36070 pycom16g15270 pycom16g15300 pycom16g15310
rosa_chinensis RchiOBHm_Chr1g0334461 RchiOBHm_Chr1g0334471 RchiOBHm_Chr2g0131731 RchiOBHm_Chr4g0417881 RchiOBHm_Chr4g0418561 RchiOBHm_Chr4g0418571 RchiOBHm_Chr4g0418581 RchiOBHm_Chr4g0418591 RchiOBHm_Chr4g0418601 RchiOBHm_Chr4g0418621 RchiOBHm_Chr4g0418651 RchiOBHm_Chr4g0418661 RchiOBHm_Chr4g0418671 RchiOBHm_Chr4g0418681 RchiOBHm_Chr4g0418691 RchiOBHm_Chr4g0418701 RchiOBHm_Chr4g0421371 RchiOBHm_Chr6g0291601 RchiOBHm_Chr6g0291611 RchiOBHm_Chr6g0291621 RchiOBHm_Chr6g0291631 RchiOBHm_Chr7g0229821
rosa_laevigata RLG00000007843 RLG00000007844 RLG00000007846 RLG00000007847 RLG00000007849 RLG00000012101 RLG00000012104 RLG00000029451
rosa_multiflora Rmu_sc0000363.1_g000035 Rmu_sc0000935.1_g000003 Rmu_sc0000935.1_g000010 Rmu_sc0002226.1_g000008 Rmu_sc0002226.1_g000014 Rmu_sc0002589.1_g000031 Rmu_sc0003443.1_g000009 Rmu_sc0006431.1_g000001 Rmu_sc0006431.1_g000006 Rmu_sc0006431.1_g000023 Rmu_sc0006705.1_g000014 Rmu_sc0006705.1_g000015 Rmu_sc0006705.1_g000016 Rmu_sc0006705.1_g000017 Rmu_sc0006705.1_g000018 Rmu_sc0014797.1_g000001 Rmu_sc0041885.1_g000001
rosa_roxburghii Rroxscaffold_4G00316820 Rroxscaffold_5G00361830 Rroxscaffold_5G00361840 Rroxscaffold_5G00361850 Rroxscaffold_5G00361860 Rroxscaffold_5G00361880 Rroxscaffold_5G00361890 Rroxscaffold_5G00361900 Rroxscaffold_5G00361910 Rroxscaffold_5G00361920 Rroxscaffold_5G00361930 Rroxscaffold_7G00175910 Rroxscaffold_7G00175930 Rroxscaffold_7G00175940
rosa_rugosa Rorug02G0299300 Rorug04G0154500 Rorug04G0154600 Rorug04G0154700 Rorug06G0220500
rosa_samantha Rh1AG006300 Rh1AG139200 Rh1AG139300 Rh1BG105600 Rh1CG131200 Rh1CG131300 Rh1DG143200 Rh2AG210000 Rh2CG337500 Rh2DG216200 Rh2DG376200 Rh4AG215900 Rh4AG216000 Rh4AG216100 Rh4AG216200 Rh4AG216300 Rh4AG216400 Rh4BG214300 Rh4BG214400 Rh4BG214500 Rh4BG214600 Rh4BG214700 Rh4BG214800 Rh4CG212300 Rh4CG227500 Rh4CG227700 Rh4CG227900 Rh4CG228000 Rh4CG228100 Rh4DG214200 Rh4DG215000 Rh4DG215200 Rh5AG059400 Rh6AG332900 Rh6AG333000 Rh6AG333100 Rh6BG339700 Rh6BG339900 Rh6CG346100 Rh6CG346200 Rh6DG333300 Rh6DG333400 Rh7AG435400 Rh7CG454500
rosa_wichuraiana Rw1G011480 Rw2G028580 Rw4G018270 Rw4G018280 Rw4G018290 Rw4G018360 Rw4G018390 Rw4G018400 Rw4G018410 Rw4G018420 Rw6G028910 Rw6G029000 Rw6G029010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 92
AccB1I GGYRCC 1 cut(s) 53
AciI CCGC 3 cut(s) 51, 78, 530
AcoI YGGCCR 1 cut(s) 58
AcuI CTGAAG 2 cut(s) 270, 555
AfaI GTAC 1 cut(s) 635
AfiI CCNNNNNNNGG 2 cut(s) 50, 302
AgsI TTSAA 9 cut(s) 155, 332, 404, 411, 423, 428, 554, 694, 706
AleI CACNNNNGTG 1 cut(s) 619
AluBI AGCT 8 cut(s) 9, 158, 175, 194, 335, 431, 509, 656
AluI AGCT 8 cut(s) 9, 158, 175, 194, 335, 431, 509, 656
Alw26I GTCTC 1 cut(s) 531
AlwNI CAGNNNCTG 1 cut(s) 197
AoxI GGCC 1 cut(s) 58
ApeKI GCWGC 6 cut(s) 18, 191, 194, 368, 431, 653
ArsI GACNNNNNNTTYG 2 cut(s) 290, 322
Asp700I GAANNNNTTC 2 cut(s) 599, 698
AspS9I GGNCC 1 cut(s) 293
AsuHPI GGTGA 1 cut(s) 640
AvaII GGWCC 1 cut(s) 293
AxyI CCTNAGG 1 cut(s) 576
BanI GGYRCC 1 cut(s) 53
BbvI GCAGC 6 cut(s) 5, 181, 203, 355, 418, 665
BceAI ACGGC 1 cut(s) 107
BcoDI GTCTC 1 cut(s) 531
BfmI CTRYAG 1 cut(s) 369
BglI GCCNNNNNGGC 1 cut(s) 674
BisI GCNGC 7 cut(s) 19, 52, 192, 195, 369, 432, 654
BlsI GCNGC 7 cut(s) 20, 53, 193, 196, 370, 433, 655
Bme18I GGWCC 1 cut(s) 293
BmgT120I GGNCC 1 cut(s) 293
BmiI GGNNCC 5 cut(s) 55, 103, 216, 388, 489
BmsI GCATC 2 cut(s) 244, 304
BplI GAGNNNNNCTC 1 cut(s) 37
BpmI CTGGAG 1 cut(s) 31
BpuEI CTTGAG 1 cut(s) 187
BsaJI CCNNGG 3 cut(s) 73, 390, 669
BsaWI WCCGGW 1 cut(s) 295
BsaXI ACNNNNNCTCC 2 cut(s) 378, 408
Bsc4I CCNNNNNNNGG 2 cut(s) 50, 302
Bse118I RCCGGY 1 cut(s) 56
Bse1I ACTGG 2 cut(s) 103, 323
Bse21I CCTNAGG 1 cut(s) 576
BseDI CCNNGG 3 cut(s) 73, 390, 669
BseGI GGATG 4 cut(s) 319, 445, 472, 528
BseLI CCNNNNNNNGG 2 cut(s) 50, 302
BseMII CTCAG 2 cut(s) 501, 567
BseNI ACTGG 2 cut(s) 103, 323
BseRI GAGGAG 1 cut(s) 686
BseXI GCAGC 6 cut(s) 5, 181, 203, 355, 418, 665
BseYI CCCAGC 1 cut(s) 197
BshFI GGCC 1 cut(s) 60
BshNI GGYRCC 1 cut(s) 53
BsiSI CCGG 2 cut(s) 57, 296
BslI CCNNNNNNNGG 2 cut(s) 50, 302
BsmAI GTCTC 1 cut(s) 531
BsnI GGCC 1 cut(s) 60
Bsp143I GATC 2 cut(s) 286, 517
BspACI CCGC 3 cut(s) 51, 78, 530
BspANI GGCC 1 cut(s) 60
BspCNI CTCAG 3 cut(s) 4, 502, 568
BspLI GGNNCC 5 cut(s) 55, 103, 216, 388, 489
BspMAI CTGCAG 1 cut(s) 373
BspT107I GGYRCC 1 cut(s) 53
BsrFI RCCGGY 1 cut(s) 56
BsrI ACTGG 2 cut(s) 103, 323
BssAI RCCGGY 1 cut(s) 56
BssECI CCNNGG 3 cut(s) 73, 390, 669
BssMI GATC 2 cut(s) 286, 517
BssT1I CCWWGG 1 cut(s) 390
Bst4CI ACNGT 4 cut(s) 463, 549, 628, 638
Bst6I CTCTTC 1 cut(s) 549
BstAPI GCANNNNNTGC 1 cut(s) 365
BstC8I GCNNGC 4 cut(s) 7, 177, 373, 417
BstDEI CTNAG 3 cut(s) 12, 510, 576
BstF5I GGATG 4 cut(s) 319, 445, 472, 528
BstKTI GATC 2 cut(s) 289, 520
BstMAI GTCTC 1 cut(s) 531
BstMBI GATC 2 cut(s) 286, 517
BstMWI GCNNNNNNNGC 4 cut(s) 15, 57, 365, 674
BstSFI CTRYAG 1 cut(s) 369
BstV1I GCAGC 6 cut(s) 5, 181, 203, 355, 418, 665
BstXI CCANNNNNNTGG 1 cut(s) 397
Bsu36I CCTNAGG 1 cut(s) 576
BsuRI GGCC 1 cut(s) 60
BtsCI GGATG 4 cut(s) 319, 445, 472, 528
BtsIMutI CAGTG 3 cut(s) 96, 459, 643
Cac8I GCNNGC 4 cut(s) 7, 177, 373, 417
CaiI CAGNNNCTG 1 cut(s) 197
Cfr10I RCCGGY 1 cut(s) 56
Cfr13I GGNCC 1 cut(s) 293
Csp6I GTAC 1 cut(s) 634
CviAII CATG 5 cut(s) 62, 290, 590, 617, 710
CviQI GTAC 1 cut(s) 634
DdeI CTNAG 3 cut(s) 12, 510, 576
DpnI GATC 2 cut(s) 288, 519
DpnII GATC 2 cut(s) 286, 517
DraI TTTAAA 1 cut(s) 339
DrdI GACNNNNNNGTC 1 cut(s) 92
DseDI GACNNNNNNGTC 1 cut(s) 92
EaeI YGGCCR 1 cut(s) 58
Eam1104I CTCTTC 1 cut(s) 549
EarI CTCTTC 1 cut(s) 549
EciI GGCGGA 1 cut(s) 519
Eco130I CCWWGG 1 cut(s) 390
Eco32I GATATC 1 cut(s) 472
Eco47I GGWCC 1 cut(s) 293
Eco57I CTGAAG 2 cut(s) 270, 555
Eco81I CCTNAGG 1 cut(s) 576
EcoRV GATATC 1 cut(s) 472
EcoT14I CCWWGG 1 cut(s) 390
ErhI CCWWGG 1 cut(s) 390
FaeI CATG 5 cut(s) 65, 293, 593, 620, 713
FaiI YATR 9 cut(s) 63, 93, 147, 243, 291, 500, 591, 618, 711
FalI AAGNNNNNCTT 2 cut(s) 686, 718
FatI CATG 5 cut(s) 61, 289, 589, 616, 709
Fnu4HI GCNGC 7 cut(s) 19, 52, 192, 195, 369, 432, 654
FokI GGATG 4 cut(s) 326, 432, 479, 535
Fsp4HI GCNGC 7 cut(s) 19, 52, 192, 195, 369, 432, 654
GluI GCNGC 7 cut(s) 19, 52, 192, 195, 369, 432, 654
GsaI CCCAGC 1 cut(s) 201
GsuI CTGGAG 1 cut(s) 31
HaeIII GGCC 1 cut(s) 60
HapII CCGG 2 cut(s) 57, 296
Hin1II CATG 5 cut(s) 65, 293, 593, 620, 713
HindIII AAGCTT 2 cut(s) 173, 333
HinfI GANTC 2 cut(s) 86, 110
HpaII CCGG 2 cut(s) 57, 296
HphI GGTGA 1 cut(s) 640
Hpy166II GTNNAC 1 cut(s) 624
Hpy188I TCNGA 4 cut(s) 13, 33, 139, 522
Hpy188III TCNNGA 7 cut(s) 83, 218, 329, 541, 604, 683, 703
Hpy8I GTNNAC 1 cut(s) 624
HpyCH4III ACNGT 4 cut(s) 463, 549, 628, 638
HpyCH4IV ACGT 1 cut(s) 141
HpyCH4V TGCA 6 cut(s) 191, 245, 317, 359, 371, 569
HpyF10VI GCNNNNNNNGC 4 cut(s) 15, 57, 365, 674
HpyF3I CTNAG 3 cut(s) 12, 510, 576
HpySE526I ACGT 1 cut(s) 141
Hsp92II CATG 5 cut(s) 65, 293, 593, 620, 713
Kzo9I GATC 2 cut(s) 286, 517
LmnI GCTCC 4 cut(s) 107, 163, 386, 506
Lsp1109I GCAGC 6 cut(s) 5, 181, 203, 355, 418, 665
LweI GCATC 2 cut(s) 244, 304
MaeII ACGT 1 cut(s) 141
MaeIII GTNAC 3 cut(s) 94, 457, 612
MalI GATC 2 cut(s) 288, 519
MboI GATC 2 cut(s) 286, 517
MboII GAAGA 4 cut(s) 119, 196, 263, 566
MluCI AATT 1 cut(s) 423
MlyI GAGTC 1 cut(s) 80
MmeI TCCRAC 2 cut(s) 56, 138
MroXI GAANNNNTTC 2 cut(s) 599, 698
MseI TTAA 6 cut(s) 228, 338, 483, 563, 582, 642
MslI CAYNNNNRTG 1 cut(s) 619
MspA1I CMGCKG 2 cut(s) 51, 194
MspI CCGG 2 cut(s) 57, 296
MwoI GCNNNNNNNGC 4 cut(s) 15, 57, 365, 674
NdeII GATC 2 cut(s) 286, 517
NlaIII CATG 5 cut(s) 65, 293, 593, 620, 713
NlaIV GGNNCC 5 cut(s) 55, 103, 216, 388, 489
NmeAIII GCCGAG 2 cut(s) 54, 694
NmuCI GTSAC 3 cut(s) 94, 457, 612
OliI CACNNNNGTG 1 cut(s) 619
PdmI GAANNNNTTC 2 cut(s) 599, 698
PfeI GAWTC 1 cut(s) 110
PkrI GCNGC 7 cut(s) 20, 53, 193, 196, 370, 433, 655
PleI GAGTC 1 cut(s) 80
PpsI GAGTC 1 cut(s) 80
PspFI CCCAGC 1 cut(s) 197
PspN4I GGNNCC 5 cut(s) 55, 103, 216, 388, 489
PspPI GGNCC 1 cut(s) 293
PstI CTGCAG 1 cut(s) 373
PstNI CAGNNNCTG 1 cut(s) 197
PvuII CAGCTG 1 cut(s) 194
RsaI GTAC 1 cut(s) 635
RsaNI GTAC 1 cut(s) 634
RseI CAYNNNNRTG 1 cut(s) 619
SaqAI TTAA 6 cut(s) 228, 338, 483, 563, 582, 642
SatI GCNGC 7 cut(s) 19, 52, 192, 195, 369, 432, 654
Sau3AI GATC 2 cut(s) 286, 517
Sau96I GGNCC 1 cut(s) 293
SchI GAGTC 1 cut(s) 80
SfaNI GCATC 2 cut(s) 244, 304
SfcI CTRYAG 1 cut(s) 369
SinI GGWCC 1 cut(s) 293
SmiMI CAYNNNNRTG 1 cut(s) 619
SmlI CTYRAG 1 cut(s) 166
SmoI CTYRAG 1 cut(s) 166
Sse9I AATT 1 cut(s) 423
SsiI CCGC 3 cut(s) 51, 78, 530
SspI AATATT 1 cut(s) 600
StyI CCWWGG 1 cut(s) 390
TaaI ACNGT 4 cut(s) 463, 549, 628, 638
TaiI ACGT 1 cut(s) 144
TaqI TCGA 1 cut(s) 66
TasI AATT 1 cut(s) 423
TauI GCSGC 1 cut(s) 54
TfiI GAWTC 1 cut(s) 110
Tru1I TTAA 6 cut(s) 228, 338, 483, 563, 582, 642
Tru9I TTAA 6 cut(s) 228, 338, 483, 563, 582, 642
TscAI CASTG 3 cut(s) 103, 466, 643
TseFI GTSAC 3 cut(s) 94, 457, 612
TseI GCWGC 6 cut(s) 18, 191, 194, 368, 431, 653
Tsp45I GTSAC 3 cut(s) 94, 457, 612
TspGWI ACGGA 1 cut(s) 438
TspRI CASTG 3 cut(s) 103, 466, 643
VpaK11BI GGWCC 1 cut(s) 293
XmnI GAANNNNTTC 2 cut(s) 599, 698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.