Rh4AG216000

hydrolase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000177
Physical Location & Seq
Forward (+)
51219828 .. 51221864
2037 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG216000.1

Sequence Viewer

Length: 753 bp
ATGTCAACTTCTGCAGCTTGCTTCGAGAACCCACCAACACTAAGCTCAACATACGGACAAGGAAGTGTCCTAGAATTTGGGGGCCTGAAGACTTACGTTACTGGCTCAACTGAATCTAAGCTTGCAGTCCTTCTAGTCTCCGATGTTTTTGGATATGAAAACCCAAATTTAAGGAAACTTGCGGATAAAGTTGCTGCAGCTGGATTTTTCGTGGTGGTTCCCGATTTCTTTTATGGCGACCCTGTGAATCTTGATAATCCTCAATTCGACCGAGAGTCCTGGTTTAAAGCTCACAGCAAGATTAGTGACTTTCTAGAAAGGAGTATATATGAAAAAGGATACGAGGATGCCAAACCAGTCCTTGCTGCTTTAAAGAGTCAAGGTGTTTCTGCAATAGGTGCTGCAGGATTTTGCTGGGGAGGGGTTGTAGTGGCAAAACTTGCAAAATCTGATGACATTAAGGCTGCTGTGATATTGCATCCTGGCCGGTTAGCAGATGAAGATATACATGAGGTTAAGGTTCATACTGCAATATTGGGAGCTGAGATTGACAAAACTTCACCCCCAGAACAATTGAAACAATTTGGGGAGATTTTATCAGTAAAGTCTGAGTTTGATAGCTTTGTGAAAATATTTCCTGGAGTGGCTCATGGATGGACAATGAGGTACAATGCAGAGGATGAATCTGCAGTCAAGAGTGCTGAAGAGGCTCATTTGGACATGTTAAATTGGTTTACCAAGTATGTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
ko00361 ko00364 ko00623 ko01100 ko01110 ko01120 ko01130 map00361 map00364 map00623 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
DLH

Protein Analysis

250

Amino Acids

27.31

Weight (kDa)

5.19

Isoelectric Point (pI)

27.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DLH PF01738 30 - 248 3.2e-30 Dienelactone hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000177)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23570 AT3G23570 AT3G23570 AT3G23600 AT3G23600
fragaria_vesca FvH4_2g23960 FvH4_2g23960 FvH4_2g23960 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14330 FvH4_6g28031 FvH4_6g29270
malus_domestica MD13G1181200.v1.1 MD13G1181300.v1.1 MD13G1181400.v1.1 MD13G1181500.v1.1 MD13G1181600.v1.1 MD13G1181700.v1.1 MD13G1181800.v1.1 MD13G1181900.v1.1 MD15G1403300.v1.1 MD15G1403400.v1.1 MD16G1181900.v1.1 MD16G1182000.v1.1 MD16G1182200.v1.1 MD16G1182300.v1.1 MD16G1182400.v1.1
prunus_persica Prupe.1G149400_v2.0.a1 Prupe.1G149600_v2.0.a1 Prupe.1G149700_v2.0.a1 Prupe.1G149800_v2.0.a1 Prupe.1G549400_v2.0.a1 Prupe.8G245600_v2.0.a1
pyrus_communis pycom13g15640 pycom13g15650 pycom13g15670 pycom13g15700 pycom15g36070 pycom16g15270 pycom16g15300 pycom16g15310
rosa_chinensis RchiOBHm_Chr1g0334461 RchiOBHm_Chr1g0334471 RchiOBHm_Chr2g0131731 RchiOBHm_Chr4g0417881 RchiOBHm_Chr4g0418561 RchiOBHm_Chr4g0418571 RchiOBHm_Chr4g0418581 RchiOBHm_Chr4g0418591 RchiOBHm_Chr4g0418601 RchiOBHm_Chr4g0418621 RchiOBHm_Chr4g0418651 RchiOBHm_Chr4g0418661 RchiOBHm_Chr4g0418671 RchiOBHm_Chr4g0418681 RchiOBHm_Chr4g0418691 RchiOBHm_Chr4g0418701 RchiOBHm_Chr4g0421371 RchiOBHm_Chr6g0291601 RchiOBHm_Chr6g0291611 RchiOBHm_Chr6g0291621 RchiOBHm_Chr6g0291631 RchiOBHm_Chr7g0229821
rosa_laevigata RLG00000007843 RLG00000007844 RLG00000007846 RLG00000007847 RLG00000007849 RLG00000012101 RLG00000012104 RLG00000029451
rosa_multiflora Rmu_sc0000363.1_g000035 Rmu_sc0000935.1_g000003 Rmu_sc0000935.1_g000010 Rmu_sc0002226.1_g000008 Rmu_sc0002226.1_g000014 Rmu_sc0002589.1_g000031 Rmu_sc0003443.1_g000009 Rmu_sc0006431.1_g000001 Rmu_sc0006431.1_g000006 Rmu_sc0006431.1_g000023 Rmu_sc0006705.1_g000014 Rmu_sc0006705.1_g000015 Rmu_sc0006705.1_g000016 Rmu_sc0006705.1_g000017 Rmu_sc0006705.1_g000018 Rmu_sc0014797.1_g000001 Rmu_sc0041885.1_g000001
rosa_roxburghii Rroxscaffold_4G00316820 Rroxscaffold_5G00361830 Rroxscaffold_5G00361840 Rroxscaffold_5G00361850 Rroxscaffold_5G00361860 Rroxscaffold_5G00361880 Rroxscaffold_5G00361890 Rroxscaffold_5G00361900 Rroxscaffold_5G00361910 Rroxscaffold_5G00361920 Rroxscaffold_5G00361930 Rroxscaffold_7G00175910 Rroxscaffold_7G00175930 Rroxscaffold_7G00175940
rosa_rugosa Rorug02G0299300 Rorug04G0154500 Rorug04G0154600 Rorug04G0154700 Rorug06G0220500
rosa_samantha Rh1AG006300 Rh1AG139200 Rh1AG139300 Rh1BG105600 Rh1CG131200 Rh1CG131300 Rh1DG143200 Rh2AG210000 Rh2CG337500 Rh2DG216200 Rh2DG376200 Rh4AG215900 Rh4AG216000 Rh4AG216100 Rh4AG216200 Rh4AG216300 Rh4AG216400 Rh4BG214300 Rh4BG214400 Rh4BG214500 Rh4BG214600 Rh4BG214700 Rh4BG214800 Rh4CG212300 Rh4CG227500 Rh4CG227700 Rh4CG227900 Rh4CG228000 Rh4CG228100 Rh4DG214200 Rh4DG215000 Rh4DG215200 Rh5AG059400 Rh6AG332900 Rh6AG333000 Rh6AG333100 Rh6BG339700 Rh6BG339900 Rh6CG346100 Rh6CG346200 Rh6DG333300 Rh6DG333400 Rh7AG435400 Rh7CG454500
rosa_wichuraiana Rw1G011480 Rw2G028580 Rw4G018270 Rw4G018280 Rw4G018290 Rw4G018360 Rw4G018390 Rw4G018400 Rw4G018410 Rw4G018420 Rw6G028910 Rw6G029000 Rw6G029010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 182
AcoI YGGCCR 1 cut(s) 484
AcsI RAATTY 2 cut(s) 74, 166
AcuI CTGAAG 2 cut(s) 107, 723
AfaI GTAC 1 cut(s) 668
AflIII ACRYGT 1 cut(s) 720
AgsI TTSAA 1 cut(s) 577
AhdI GACNNNNNGTC 1 cut(s) 274
AjnI CCWGG 3 cut(s) 278, 481, 637
AluBI AGCT 7 cut(s) 17, 45, 121, 200, 290, 542, 621
AluI AGCT 7 cut(s) 17, 45, 121, 200, 290, 542, 621
Alw26I GTCTC 1 cut(s) 142
AoxI GGCC 2 cut(s) 82, 484
ApeKI GCWGC 6 cut(s) 14, 194, 197, 365, 401, 464
ApoI RAATTY 2 cut(s) 74, 166
AspS9I GGNCC 1 cut(s) 82
AsuHPI GGTGA 1 cut(s) 552
BbsI GAAGAC 1 cut(s) 95
BbvI GCAGC 6 cut(s) 26, 181, 209, 352, 388, 451
BccI CCATC 1 cut(s) 648
BciT130I CCWGG 3 cut(s) 280, 483, 639
BciVI GTATCC 1 cut(s) 332
BcoDI GTCTC 1 cut(s) 142
BfaI CTAG 3 cut(s) 71, 134, 314
BfmI CTRYAG 4 cut(s) 12, 195, 402, 687
BfuI GTATCC 1 cut(s) 332
BisI GCNGC 6 cut(s) 15, 195, 198, 366, 402, 465
BlsI GCNGC 6 cut(s) 16, 196, 199, 367, 403, 466
Bme1390I CCNGG 3 cut(s) 280, 483, 639
BmeRI GACNNNNNGTC 1 cut(s) 274
BmgT120I GGNCC 1 cut(s) 82
BmiI GGNNCC 2 cut(s) 83, 219
BmrFI CCNGG 3 cut(s) 280, 483, 639
BmsI GCATC 2 cut(s) 337, 487
BpiI GAAGAC 1 cut(s) 95
BpmI CTGGAG 1 cut(s) 660
BsaXI ACNNNNNCTCC 2 cut(s) 411, 441
Bse118I RCCGGY 1 cut(s) 486
Bse1I ACTGG 2 cut(s) 106, 356
BseBI CCWGG 3 cut(s) 280, 483, 639
BseGI GGATG 4 cut(s) 352, 478, 659, 685
BseMII CTCAG 2 cut(s) 534, 600
BseNI ACTGG 2 cut(s) 106, 356
BseXI GCAGC 6 cut(s) 26, 181, 209, 352, 388, 451
BseYI CCCAGC 1 cut(s) 414
Bsh1285I CGRYCG 1 cut(s) 271
BshFI GGCC 2 cut(s) 84, 486
BsiEI CGRYCG 1 cut(s) 271
BsiSI CCGG 1 cut(s) 487
BsmAI GTCTC 1 cut(s) 142
BsnI GGCC 2 cut(s) 84, 486
BspACI CCGC 1 cut(s) 182
BspANI GGCC 2 cut(s) 84, 486
BspCNI CTCAG 2 cut(s) 535, 601
BspLI GGNNCC 2 cut(s) 83, 219
BspMAI CTGCAG 4 cut(s) 16, 199, 406, 691
BsrFI RCCGGY 1 cut(s) 486
BsrI ACTGG 2 cut(s) 106, 356
BssAI RCCGGY 1 cut(s) 486
Bst2UI CCWGG 3 cut(s) 280, 483, 639
Bst6I CTCTTC 1 cut(s) 699
BstAPI GCANNNNNTGC 2 cut(s) 398, 440
BstC8I GCNNGC 2 cut(s) 19, 123
BstDEI CTNAG 4 cut(s) 41, 117, 543, 609
BstF5I GGATG 4 cut(s) 352, 478, 659, 685
BstMAI GTCTC 1 cut(s) 142
BstMCI CGRYCG 1 cut(s) 271
BstMWI GCNNNNNNNGC 3 cut(s) 398, 440, 707
BstNI CCWGG 3 cut(s) 280, 483, 639
BstNSI RCATGY 1 cut(s) 724
BstSCI CCNGG 3 cut(s) 278, 481, 637
BstSFI CTRYAG 4 cut(s) 12, 195, 402, 687
BstV1I GCAGC 6 cut(s) 26, 181, 209, 352, 388, 451
BstV2I GAAGAC 1 cut(s) 95
BsuI GTATCC 1 cut(s) 332
BsuRI GGCC 2 cut(s) 84, 486
BtsCI GGATG 4 cut(s) 352, 478, 659, 685
Cac8I GCNNGC 2 cut(s) 19, 123
Cfr10I RCCGGY 1 cut(s) 486
Cfr13I GGNCC 1 cut(s) 82
Csp6I GTAC 1 cut(s) 667
CviAII CATG 3 cut(s) 509, 650, 721
CviQI GTAC 1 cut(s) 667
DdeI CTNAG 4 cut(s) 41, 117, 543, 609
DraI TTTAAA 2 cut(s) 286, 372
DriI GACNNNNNGTC 1 cut(s) 274
EaeI YGGCCR 1 cut(s) 484
Eam1104I CTCTTC 1 cut(s) 699
Eam1105I GACNNNNNGTC 1 cut(s) 274
EarI CTCTTC 1 cut(s) 699
Eco57I CTGAAG 2 cut(s) 107, 723
EcoO109I RGGNCCY 1 cut(s) 82
EcoRII CCWGG 3 cut(s) 278, 481, 637
FaeI CATG 3 cut(s) 512, 653, 724
FatI CATG 3 cut(s) 508, 649, 720
Fnu4HI GCNGC 6 cut(s) 15, 195, 198, 366, 402, 465
FokI GGATG 4 cut(s) 359, 465, 666, 692
Fsp4HI GCNGC 6 cut(s) 15, 195, 198, 366, 402, 465
FspBI CTAG 3 cut(s) 71, 134, 314
GluI GCNGC 6 cut(s) 15, 195, 198, 366, 402, 465
GsaI CCCAGC 1 cut(s) 418
GsuI CTGGAG 1 cut(s) 660
HaeIII GGCC 2 cut(s) 84, 486
HapII CCGG 1 cut(s) 487
Hin1II CATG 3 cut(s) 512, 653, 724
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HindIII AAGCTT 1 cut(s) 119
HinfI GANTC 5 cut(s) 113, 247, 275, 376, 683
HpaII CCGG 1 cut(s) 487
HphI GGTGA 1 cut(s) 552
Hpy166II GTNNAC 2 cut(s) 6, 735
Hpy188I TCNGA 3 cut(s) 142, 451, 610
Hpy188III TCNNGA 5 cut(s) 25, 221, 251, 314, 694
Hpy8I GTNNAC 2 cut(s) 6, 735
HpyAV CCTTC 1 cut(s) 140
HpyCH4IV ACGT 1 cut(s) 96
HpyF10VI GCNNNNNNNGC 3 cut(s) 398, 440, 707
HpyF3I CTNAG 4 cut(s) 41, 117, 543, 609
HpySE526I ACGT 1 cut(s) 96
Hsp92II CATG 3 cut(s) 512, 653, 724
LmnI GCTCC 1 cut(s) 539
Lsp1109I GCAGC 6 cut(s) 26, 181, 209, 352, 388, 451
LweI GCATC 2 cut(s) 337, 487
MaeI CTAG 3 cut(s) 71, 134, 314
MaeII ACGT 1 cut(s) 96
MaeIII GTNAC 2 cut(s) 97, 305
MboII GAAGA 3 cut(s) 100, 512, 716
MfeI CAATTG 1 cut(s) 572
MluCI AATT 6 cut(s) 74, 166, 263, 572, 581, 727
MlyI GAGTC 2 cut(s) 284, 385
MnlI CCTC 7 cut(s) 270, 337, 413, 505, 657, 670, 700
MseI TTAA 6 cut(s) 170, 285, 371, 459, 516, 725
MspA1I CMGCKG 1 cut(s) 200
MspI CCGG 1 cut(s) 487
MspR9I CCNGG 3 cut(s) 280, 483, 639
MunI CAATTG 1 cut(s) 572
MvaI CCWGG 3 cut(s) 280, 483, 639
MwoI GCNNNNNNNGC 3 cut(s) 398, 440, 707
NlaIII CATG 3 cut(s) 512, 653, 724
NlaIV GGNNCC 2 cut(s) 83, 219
NmuCI GTSAC 1 cut(s) 305
NspI RCATGY 1 cut(s) 724
PciI ACATGT 1 cut(s) 720
PfeI GAWTC 3 cut(s) 113, 247, 683
PfoI TCCNGGA 1 cut(s) 637
PkrI GCNGC 6 cut(s) 16, 196, 199, 367, 403, 466
PleI GAGTC 2 cut(s) 283, 384
PpsI GAGTC 2 cut(s) 283, 384
PscI ACATGT 1 cut(s) 720
Psp6I CCWGG 3 cut(s) 278, 481, 637
PspFI CCCAGC 1 cut(s) 414
PspGI CCWGG 3 cut(s) 278, 481, 637
PspN4I GGNNCC 2 cut(s) 83, 219
PspPI GGNCC 1 cut(s) 82
PstI CTGCAG 4 cut(s) 16, 199, 406, 691
PvuII CAGCTG 1 cut(s) 200
RsaI GTAC 1 cut(s) 668
RsaNI GTAC 1 cut(s) 667
SaqAI TTAA 6 cut(s) 170, 285, 371, 459, 516, 725
SatI GCNGC 6 cut(s) 15, 195, 198, 366, 402, 465
Sau96I GGNCC 1 cut(s) 82
SchI GAGTC 2 cut(s) 284, 385
ScrFI CCNGG 3 cut(s) 280, 483, 639
SfaNI GCATC 2 cut(s) 337, 487
SfcI CTRYAG 4 cut(s) 12, 195, 402, 687
Sse9I AATT 6 cut(s) 74, 166, 263, 572, 581, 727
SsiI CCGC 1 cut(s) 182
SspI AATATT 2 cut(s) 534, 633
SspMI CTAG 3 cut(s) 71, 134, 314
StyD4I CCNGG 3 cut(s) 278, 481, 637
TaiI ACGT 1 cut(s) 99
TaqI TCGA 2 cut(s) 24, 267
TaqII GACCGA 1 cut(s) 285
TasI AATT 6 cut(s) 74, 166, 263, 572, 581, 727
TfiI GAWTC 3 cut(s) 113, 247, 683
Tru1I TTAA 6 cut(s) 170, 285, 371, 459, 516, 725
Tru9I TTAA 6 cut(s) 170, 285, 371, 459, 516, 725
TseFI GTSAC 1 cut(s) 305
TseI GCWGC 6 cut(s) 14, 194, 197, 365, 401, 464
Tsp45I GTSAC 1 cut(s) 305
TspDTI ATGAA 5 cut(s) 171, 345, 512, 513, 696
TspGWI ACGGA 1 cut(s) 69
XapI RAATTY 2 cut(s) 74, 166
XbaI TCTAGA 1 cut(s) 313
XceI RCATGY 1 cut(s) 724
XspI CTAG 3 cut(s) 71, 134, 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.