RchiOBHm_Chr4g0418701

1,4-beta-D-glucanase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000177
Physical Location & Seq
Forward (+)
44156429 .. 44159424
2996 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38868

Sequence Viewer

Length: 735 bp
ATGTCAGGCCCTGCGTGCTGCTCAAACCCTCCAGCCCTTAACCCAGCAAGTGGTTCCGGCCATGTTGAAAAGCTTGCTGGTCTGGACTCCTATGTCACTGGCTCTCCTGACTCCAAGCTTGCCGTTCTTCTCATTGCTGACATTTTTGGCTATGAAGCTCCACTCTTGAGGAAGCTCGCAGACAAAGTTGCAGCTGCTGGGTTCTTTGTTGTGGTACCTGATTTCTTTTATGGAGATGCTTTTCCTGGTGATGCCGGACCAGTTGGAAACTCTTCTGCTCTACCTATTTGGTTAAAGACACATTTACCGATCAAGGCATCTGAAGCTGCAAAACCATTACTTGAAGCCTTAAAGAGTAAAGGTGTTTCTGCAATTGGGGCTGTAGGCTTCTGTTGGGGAGCCAAGGCTGCGGTTGAACTTGCAAAGTATGATGACCTTCTCCATGCTGCTGTCTTGTGCCATCCTTCATTCGTCACTGTGGACGATTTCAAGGCGGTTAAGGTTCCCATTTCTGTACTTGGAGCTGAGATTGATCCGATTTCGCCACCTGACGTCGTGAAACAATTTGAAGAGGTTTTAACTGCAAGACCTGAGATCAAAAGCCATGTGAAGATATTCCCAAAAGTGCAACATGGGTGGACCGTGAGGTACAACGCTGAAGATGAGGCTGCTGTTAAGTGTGCTGAGGAGGCTCATCAAGACATACTGGATTGGTTCTTTAGCCATGTCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
ko00361 ko00364 ko00623 ko01100 ko01110 ko01120 ko01130 map00361 map00364 map00623 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
DLH

Protein Analysis

244

Amino Acids

25.93

Weight (kDa)

5.59

Isoelectric Point (pI)

31.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DLH PF01738 29 - 240 1.4e-31 Dienelactone hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000177)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23570 AT3G23570 AT3G23570 AT3G23600 AT3G23600
fragaria_vesca FvH4_2g23960 FvH4_2g23960 FvH4_2g23960 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14330 FvH4_6g28031 FvH4_6g29270
malus_domestica MD13G1181200.v1.1 MD13G1181300.v1.1 MD13G1181400.v1.1 MD13G1181500.v1.1 MD13G1181600.v1.1 MD13G1181700.v1.1 MD13G1181800.v1.1 MD13G1181900.v1.1 MD15G1403300.v1.1 MD15G1403400.v1.1 MD16G1181900.v1.1 MD16G1182000.v1.1 MD16G1182200.v1.1 MD16G1182300.v1.1 MD16G1182400.v1.1
prunus_persica Prupe.1G149400_v2.0.a1 Prupe.1G149600_v2.0.a1 Prupe.1G149700_v2.0.a1 Prupe.1G149800_v2.0.a1 Prupe.1G549400_v2.0.a1 Prupe.8G245600_v2.0.a1
pyrus_communis pycom13g15640 pycom13g15650 pycom13g15670 pycom13g15700 pycom15g36070 pycom16g15270 pycom16g15300 pycom16g15310
rosa_chinensis RchiOBHm_Chr1g0334461 RchiOBHm_Chr1g0334471 RchiOBHm_Chr2g0131731 RchiOBHm_Chr4g0417881 RchiOBHm_Chr4g0418561 RchiOBHm_Chr4g0418571 RchiOBHm_Chr4g0418581 RchiOBHm_Chr4g0418591 RchiOBHm_Chr4g0418601 RchiOBHm_Chr4g0418621 RchiOBHm_Chr4g0418651 RchiOBHm_Chr4g0418661 RchiOBHm_Chr4g0418671 RchiOBHm_Chr4g0418681 RchiOBHm_Chr4g0418691 RchiOBHm_Chr4g0418701 RchiOBHm_Chr4g0421371 RchiOBHm_Chr6g0291601 RchiOBHm_Chr6g0291611 RchiOBHm_Chr6g0291621 RchiOBHm_Chr6g0291631 RchiOBHm_Chr7g0229821
rosa_laevigata RLG00000007843 RLG00000007844 RLG00000007846 RLG00000007847 RLG00000007849 RLG00000012101 RLG00000012104 RLG00000029451
rosa_multiflora Rmu_sc0000363.1_g000035 Rmu_sc0000935.1_g000003 Rmu_sc0000935.1_g000010 Rmu_sc0002226.1_g000008 Rmu_sc0002226.1_g000014 Rmu_sc0002589.1_g000031 Rmu_sc0003443.1_g000009 Rmu_sc0006431.1_g000001 Rmu_sc0006431.1_g000006 Rmu_sc0006431.1_g000023 Rmu_sc0006705.1_g000014 Rmu_sc0006705.1_g000015 Rmu_sc0006705.1_g000016 Rmu_sc0006705.1_g000017 Rmu_sc0006705.1_g000018 Rmu_sc0014797.1_g000001 Rmu_sc0041885.1_g000001
rosa_roxburghii Rroxscaffold_4G00316820 Rroxscaffold_5G00361830 Rroxscaffold_5G00361840 Rroxscaffold_5G00361850 Rroxscaffold_5G00361860 Rroxscaffold_5G00361880 Rroxscaffold_5G00361890 Rroxscaffold_5G00361900 Rroxscaffold_5G00361910 Rroxscaffold_5G00361920 Rroxscaffold_5G00361930 Rroxscaffold_7G00175910 Rroxscaffold_7G00175930 Rroxscaffold_7G00175940
rosa_rugosa Rorug02G0299300 Rorug04G0154500 Rorug04G0154600 Rorug04G0154700 Rorug06G0220500
rosa_samantha Rh1AG006300 Rh1AG139200 Rh1AG139300 Rh1BG105600 Rh1CG131200 Rh1CG131300 Rh1DG143200 Rh2AG210000 Rh2CG337500 Rh2DG216200 Rh2DG376200 Rh4AG215900 Rh4AG216000 Rh4AG216100 Rh4AG216200 Rh4AG216300 Rh4AG216400 Rh4BG214300 Rh4BG214400 Rh4BG214500 Rh4BG214600 Rh4BG214700 Rh4BG214800 Rh4CG212300 Rh4CG227500 Rh4CG227700 Rh4CG227900 Rh4CG228000 Rh4CG228100 Rh4DG214200 Rh4DG215000 Rh4DG215200 Rh5AG059400 Rh6AG332900 Rh6AG333000 Rh6AG333100 Rh6BG339700 Rh6BG339900 Rh6CG346100 Rh6CG346200 Rh6DG333300 Rh6DG333400 Rh7AG435400 Rh7CG454500
rosa_wichuraiana Rw1G011480 Rw2G028580 Rw4G018270 Rw4G018280 Rw4G018290 Rw4G018360 Rw4G018390 Rw4G018400 Rw4G018410 Rw4G018420 Rw6G028910 Rw6G029000 Rw6G029010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 92
AatII GACGTC 1 cut(s) 555
Acc65I GGTACC 1 cut(s) 214
AccB1I GGYRCC 1 cut(s) 214
AccB7I CCANNNNNTGG 1 cut(s) 50
AciI CCGC 2 cut(s) 410, 494
AclWI GGATC 1 cut(s) 527
AcoI YGGCCR 1 cut(s) 58
AcuI CTGAAG 2 cut(s) 342, 678
AcyI GRCGYC 1 cut(s) 552
AfaI GTAC 3 cut(s) 216, 516, 650
AfiI CCNNNNNNNGG 1 cut(s) 50
AgsI TTSAA 5 cut(s) 68, 344, 416, 490, 569
AjnI CCWGG 1 cut(s) 244
AluBI AGCT 7 cut(s) 73, 118, 158, 175, 194, 326, 524
AluI AGCT 7 cut(s) 73, 118, 158, 175, 194, 326, 524
AlwI GGATC 1 cut(s) 527
AlwNI CAGNNNCTG 2 cut(s) 11, 197
AoxI GGCC 2 cut(s) 7, 58
ApeKI GCWGC 7 cut(s) 18, 191, 194, 326, 407, 446, 668
Asp700I GAANNNNTTC 2 cut(s) 271, 614
Asp718I GGTACC 1 cut(s) 214
AspS9I GGNCC 3 cut(s) 8, 257, 639
AsuHPI GGTGA 1 cut(s) 260
AvaII GGWCC 2 cut(s) 257, 639
BanI GGYRCC 1 cut(s) 214
BbvCI CCTCAGC 1 cut(s) 684
BbvI GCAGC 7 cut(s) 5, 181, 203, 313, 394, 433, 655
BccI CCATC 1 cut(s) 468
BceAI ACGGC 1 cut(s) 107
BciT130I CCWGG 1 cut(s) 246
BfmI CTRYAG 1 cut(s) 381
BisI GCNGC 7 cut(s) 19, 192, 195, 327, 408, 447, 669
BlsI GCNGC 7 cut(s) 20, 193, 196, 328, 409, 448, 670
Bme1390I CCNGG 1 cut(s) 246
Bme18I GGWCC 2 cut(s) 257, 639
BmgT120I GGNCC 3 cut(s) 8, 257, 639
BmiI GGNNCC 4 cut(s) 55, 216, 400, 504
BmrFI CCNGG 1 cut(s) 246
BmsI GCATC 3 cut(s) 226, 241, 326
BpmI CTGGAG 1 cut(s) 15
Bpu10I CCTNAGC 1 cut(s) 684
BpuEI CTTGAG 1 cut(s) 187
BsaHI GRCGYC 1 cut(s) 552
BsaJI CCNNGG 1 cut(s) 402
BsaXI ACNNNNNCTCC 2 cut(s) 88, 118
Bsc4I CCNNNNNNNGG 1 cut(s) 50
Bse1I ACTGG 3 cut(s) 103, 260, 711
Bse3DI GCAATG 1 cut(s) 132
BseBI CCWGG 1 cut(s) 246
BseDI CCNNGG 1 cut(s) 402
BseGI GGATG 1 cut(s) 460
BseLI CCNNNNNNNGG 1 cut(s) 50
BseMI GCAATG 1 cut(s) 132
BseMII CTCAG 3 cut(s) 516, 582, 675
BseNI ACTGG 3 cut(s) 103, 260, 711
BseRI GAGGAG 1 cut(s) 701
BseXI GCAGC 7 cut(s) 5, 181, 203, 313, 394, 433, 655
BseYI CCCAGC 2 cut(s) 43, 197
BshFI GGCC 2 cut(s) 9, 60
BshNI GGYRCC 1 cut(s) 214
BsiSI CCGG 2 cut(s) 57, 255
BslI CCNNNNNNNGG 1 cut(s) 50
BsnI GGCC 2 cut(s) 9, 60
Bsp143I GATC 3 cut(s) 309, 532, 594
BspACI CCGC 2 cut(s) 410, 494
BspANI GGCC 2 cut(s) 9, 60
BspCNI CTCAG 3 cut(s) 517, 583, 676
BspLI GGNNCC 4 cut(s) 55, 216, 400, 504
BspPI GGATC 1 cut(s) 527
BspT107I GGYRCC 1 cut(s) 214
BsrDI GCAATG 1 cut(s) 132
BsrI ACTGG 3 cut(s) 103, 260, 711
BssECI CCNNGG 1 cut(s) 402
BssMI GATC 3 cut(s) 309, 532, 594
BssNI GRCGYC 1 cut(s) 552
BssT1I CCWWGG 1 cut(s) 402
Bst2UI CCWGG 1 cut(s) 246
Bst4CI ACNGT 2 cut(s) 478, 643
Bst6I CTCTTC 2 cut(s) 277, 564
BstACI GRCGYC 1 cut(s) 552
BstC8I GCNNGC 4 cut(s) 16, 75, 120, 177
BstDEI CTNAG 3 cut(s) 525, 591, 684
BstF5I GGATG 1 cut(s) 460
BstKTI GATC 3 cut(s) 312, 535, 597
BstMBI GATC 3 cut(s) 309, 532, 594
BstMWI GCNNNNNNNGC 5 cut(s) 15, 323, 377, 407, 689
BstNI CCWGG 1 cut(s) 246
BstSCI CCNGG 1 cut(s) 244
BstSFI CTRYAG 1 cut(s) 381
BstV1I GCAGC 7 cut(s) 5, 181, 203, 313, 394, 433, 655
BsuRI GGCC 2 cut(s) 9, 60
BtsCI GGATG 1 cut(s) 460
BtsIMutI CAGTG 2 cut(s) 96, 474
Cac8I GCNNGC 4 cut(s) 16, 75, 120, 177
CaiI CAGNNNCTG 2 cut(s) 11, 197
Cfr13I GGNCC 3 cut(s) 8, 257, 639
Csp6I GTAC 3 cut(s) 215, 515, 649
CspCI CAANNNNNGTGG 2 cut(s) 617, 652
CviAII CATG 5 cut(s) 62, 443, 605, 632, 725
CviQI GTAC 3 cut(s) 215, 515, 649
DdeI CTNAG 3 cut(s) 525, 591, 684
DpnI GATC 3 cut(s) 311, 534, 596
DpnII GATC 3 cut(s) 309, 532, 594
DrdI GACNNNNNNGTC 1 cut(s) 92
DseDI GACNNNNNNGTC 1 cut(s) 92
EaeI YGGCCR 1 cut(s) 58
Eam1104I CTCTTC 2 cut(s) 277, 564
EarI CTCTTC 2 cut(s) 277, 564
Eco130I CCWWGG 1 cut(s) 402
Eco47I GGWCC 2 cut(s) 257, 639
Eco57I CTGAAG 2 cut(s) 342, 678
EcoO109I RGGNCCY 1 cut(s) 8
EcoRII CCWGG 1 cut(s) 244
EcoT14I CCWWGG 1 cut(s) 402
ErhI CCWWGG 1 cut(s) 402
FaeI CATG 5 cut(s) 65, 446, 608, 635, 728
FatI CATG 5 cut(s) 61, 442, 604, 631, 724
Fnu4HI GCNGC 7 cut(s) 19, 192, 195, 327, 408, 447, 669
FokI GGATG 1 cut(s) 447
Fsp4HI GCNGC 7 cut(s) 19, 192, 195, 327, 408, 447, 669
GluI GCNGC 7 cut(s) 19, 192, 195, 327, 408, 447, 669
GsaI CCCAGC 2 cut(s) 47, 201
GsuI CTGGAG 1 cut(s) 15
HaeIII GGCC 2 cut(s) 9, 60
HapII CCGG 2 cut(s) 57, 255
Hin1I GRCGYC 1 cut(s) 552
Hin1II CATG 5 cut(s) 65, 446, 608, 635, 728
HindIII AAGCTT 2 cut(s) 71, 116
HinfI GANTC 2 cut(s) 86, 110
HpaII CCGG 2 cut(s) 57, 255
HphI GGTGA 1 cut(s) 260
Hpy166II GTNNAC 2 cut(s) 481, 639
Hpy188I TCNGA 2 cut(s) 322, 537
Hpy188III TCNNGA 5 cut(s) 83, 107, 166, 556, 698
Hpy8I GTNNAC 2 cut(s) 481, 639
Hpy99I CGWCG 1 cut(s) 557
HpyAV CCTTC 2 cut(s) 446, 474
HpyCH4III ACNGT 2 cut(s) 478, 643
HpyCH4IV ACGT 1 cut(s) 552
HpyCH4V TGCA 6 cut(s) 191, 329, 371, 422, 584, 628
HpyF10VI GCNNNNNNNGC 5 cut(s) 15, 323, 377, 407, 689
HpyF3I CTNAG 3 cut(s) 525, 591, 684
HpySE526I ACGT 1 cut(s) 552
Hsp92I GRCGYC 1 cut(s) 552
Hsp92II CATG 5 cut(s) 65, 446, 608, 635, 728
KpnI GGTACC 1 cut(s) 218
Kzo9I GATC 3 cut(s) 309, 532, 594
LmnI GCTCC 3 cut(s) 163, 398, 521
Lsp1109I GCAGC 7 cut(s) 5, 181, 203, 313, 394, 433, 655
LweI GCATC 3 cut(s) 226, 241, 326
MaeII ACGT 1 cut(s) 552
MaeIII GTNAC 2 cut(s) 94, 472
MalI GATC 3 cut(s) 311, 534, 596
MboI GATC 3 cut(s) 309, 532, 594
MboII GAAGA 5 cut(s) 119, 264, 581, 622, 671
MfeI CAATTG 1 cut(s) 372
MluCI AATT 2 cut(s) 372, 563
MlyI GAGTC 2 cut(s) 80, 104
MmeI TCCRAC 1 cut(s) 244
MnlI CCTC 7 cut(s) 39, 162, 565, 639, 658, 679, 682
MroXI GAANNNNTTC 2 cut(s) 271, 614
MseI TTAA 6 cut(s) 39, 293, 350, 498, 578, 675
MspA1I CMGCKG 1 cut(s) 194
MspI CCGG 2 cut(s) 57, 255
MspR9I CCNGG 1 cut(s) 246
MunI CAATTG 1 cut(s) 372
MvaI CCWGG 1 cut(s) 246
MwoI GCNNNNNNNGC 5 cut(s) 15, 323, 377, 407, 689
NdeII GATC 3 cut(s) 309, 532, 594
NlaIII CATG 5 cut(s) 65, 446, 608, 635, 728
NlaIV GGNNCC 4 cut(s) 55, 216, 400, 504
NmuCI GTSAC 2 cut(s) 94, 472
PdmI GAANNNNTTC 2 cut(s) 271, 614
PflMI CCANNNNNTGG 1 cut(s) 50
PkrI GCNGC 7 cut(s) 20, 193, 196, 328, 409, 448, 670
PleI GAGTC 2 cut(s) 80, 104
PpsI GAGTC 2 cut(s) 80, 104
Psp6I CCWGG 1 cut(s) 244
PspFI CCCAGC 2 cut(s) 43, 197
PspGI CCWGG 1 cut(s) 244
PspN4I GGNNCC 4 cut(s) 55, 216, 400, 504
PspPI GGNCC 3 cut(s) 8, 257, 639
PstNI CAGNNNCTG 2 cut(s) 11, 197
PvuII CAGCTG 1 cut(s) 194
RsaI GTAC 3 cut(s) 216, 516, 650
RsaNI GTAC 3 cut(s) 215, 515, 649
SaqAI TTAA 6 cut(s) 39, 293, 350, 498, 578, 675
SatI GCNGC 7 cut(s) 19, 192, 195, 327, 408, 447, 669
Sau3AI GATC 3 cut(s) 309, 532, 594
Sau96I GGNCC 3 cut(s) 8, 257, 639
SchI GAGTC 2 cut(s) 80, 104
ScrFI CCNGG 1 cut(s) 246
SfaNI GCATC 3 cut(s) 226, 241, 326
SfcI CTRYAG 1 cut(s) 381
SinI GGWCC 2 cut(s) 257, 639
SmlI CTYRAG 1 cut(s) 166
SmoI CTYRAG 1 cut(s) 166
Sse9I AATT 2 cut(s) 372, 563
SsiI CCGC 2 cut(s) 410, 494
StyD4I CCNGG 1 cut(s) 244
StyI CCWWGG 1 cut(s) 402
TaaI ACNGT 2 cut(s) 478, 643
TaiI ACGT 1 cut(s) 555
TasI AATT 2 cut(s) 372, 563
TatI WGTACW 1 cut(s) 514
Tru1I TTAA 6 cut(s) 39, 293, 350, 498, 578, 675
Tru9I TTAA 6 cut(s) 39, 293, 350, 498, 578, 675
TscAI CASTG 2 cut(s) 103, 481
TseFI GTSAC 2 cut(s) 94, 472
TseI GCWGC 7 cut(s) 18, 191, 194, 326, 407, 446, 668
Tsp45I GTSAC 2 cut(s) 94, 472
TspDTI ATGAA 2 cut(s) 168, 456
TspRI CASTG 2 cut(s) 103, 481
Van91I CCANNNNNTGG 1 cut(s) 50
VpaK11BI GGWCC 2 cut(s) 257, 639
XmnI GAANNNNTTC 2 cut(s) 271, 614
ZraI GACGTC 1 cut(s) 553
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.