Rw4G018280

hydrolase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000177
Physical Location & Seq
Forward (+)
40654025 .. 40656109
2085 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G018280.1

Sequence Viewer

Length: 723 bp
ATGTCAACTTCTGCAGCTTGCTTCGAGAACCCACCAACACTAAGCTCAACATACGGAAAAGGAACTGTCCTAGAATTTGGGGGCCTGAAGACTTACGTTACTGGCTCAACTGAATCTAAGCTTGCAGTCCTTCTAGTCTCCGATGTTTTTGGATATGAAAACCCAAATATAAGGAAACTTGCGGATAAAGTTGCTGCAGCTGGATTTTTCGTGGTGGTTCCCGATTTCTTTTATGGCGACCCTGTGAATTTTGATAATCCTCAATTCGACCGAGAGTCCTGGTTAATAGCTCACAGCAAGGAAAAAGGATACGAGGATTCTAAATCAGTCCTTGCTGCATTAAAGAGTCAAGGTGTTTCTGCAATAGGAGCTGCAGGGTTTTGCTGGGGAGGGGTTGTAGTGGCAAAACTTGCAAAATCTGATGACATTAAGGCTGCTGTGATATTGCATCCTGGCCGGTTAGCAGATGAAGATATACATGAGGTTAAGGTTCATACTGCAATATTGGGAGCTGAGATTGACAAAACTTCACCACCAGAACAATTGAAACAATTTGAGGAGATTTTATCAGTGAAGTCTGAGTTTGATAGCTTTGTGAAAATATTTCCTGGAGTGGCTCATGGATGGACAATGAGGTACAATGCAGAGGATGAATCTGCAGTCAAGAGTGCTGAAGAGGCTCATTTGGACATGTTAAATTGGTTTACCAAGTATGTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
ko00361 ko00364 ko00623 ko01100 ko01110 ko01120 ko01130 map00361 map00364 map00623 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
DLH

Protein Analysis

240

Amino Acids

26.16

Weight (kDa)

5.19

Isoelectric Point (pI)

30.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DLH PF01738 30 - 238 1.2e-31 Dienelactone hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000177)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23570 AT3G23570 AT3G23570 AT3G23600 AT3G23600
fragaria_vesca FvH4_2g23960 FvH4_2g23960 FvH4_2g23960 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14330 FvH4_6g28031 FvH4_6g29270
malus_domestica MD13G1181200.v1.1 MD13G1181300.v1.1 MD13G1181400.v1.1 MD13G1181500.v1.1 MD13G1181600.v1.1 MD13G1181700.v1.1 MD13G1181800.v1.1 MD13G1181900.v1.1 MD15G1403300.v1.1 MD15G1403400.v1.1 MD16G1181900.v1.1 MD16G1182000.v1.1 MD16G1182200.v1.1 MD16G1182300.v1.1 MD16G1182400.v1.1
prunus_persica Prupe.1G149400_v2.0.a1 Prupe.1G149600_v2.0.a1 Prupe.1G149700_v2.0.a1 Prupe.1G149800_v2.0.a1 Prupe.1G549400_v2.0.a1 Prupe.8G245600_v2.0.a1
pyrus_communis pycom13g15640 pycom13g15650 pycom13g15670 pycom13g15700 pycom15g36070 pycom16g15270 pycom16g15300 pycom16g15310
rosa_chinensis RchiOBHm_Chr1g0334461 RchiOBHm_Chr1g0334471 RchiOBHm_Chr2g0131731 RchiOBHm_Chr4g0417881 RchiOBHm_Chr4g0418561 RchiOBHm_Chr4g0418571 RchiOBHm_Chr4g0418581 RchiOBHm_Chr4g0418591 RchiOBHm_Chr4g0418601 RchiOBHm_Chr4g0418621 RchiOBHm_Chr4g0418651 RchiOBHm_Chr4g0418661 RchiOBHm_Chr4g0418671 RchiOBHm_Chr4g0418681 RchiOBHm_Chr4g0418691 RchiOBHm_Chr4g0418701 RchiOBHm_Chr4g0421371 RchiOBHm_Chr6g0291601 RchiOBHm_Chr6g0291611 RchiOBHm_Chr6g0291621 RchiOBHm_Chr6g0291631 RchiOBHm_Chr7g0229821
rosa_laevigata RLG00000007843 RLG00000007844 RLG00000007846 RLG00000007847 RLG00000007849 RLG00000012101 RLG00000012104 RLG00000029451
rosa_multiflora Rmu_sc0000363.1_g000035 Rmu_sc0000935.1_g000003 Rmu_sc0000935.1_g000010 Rmu_sc0002226.1_g000008 Rmu_sc0002226.1_g000014 Rmu_sc0002589.1_g000031 Rmu_sc0003443.1_g000009 Rmu_sc0006431.1_g000001 Rmu_sc0006431.1_g000006 Rmu_sc0006431.1_g000023 Rmu_sc0006705.1_g000014 Rmu_sc0006705.1_g000015 Rmu_sc0006705.1_g000016 Rmu_sc0006705.1_g000017 Rmu_sc0006705.1_g000018 Rmu_sc0014797.1_g000001 Rmu_sc0041885.1_g000001
rosa_roxburghii Rroxscaffold_4G00316820 Rroxscaffold_5G00361830 Rroxscaffold_5G00361840 Rroxscaffold_5G00361850 Rroxscaffold_5G00361860 Rroxscaffold_5G00361880 Rroxscaffold_5G00361890 Rroxscaffold_5G00361900 Rroxscaffold_5G00361910 Rroxscaffold_5G00361920 Rroxscaffold_5G00361930 Rroxscaffold_7G00175910 Rroxscaffold_7G00175930 Rroxscaffold_7G00175940
rosa_rugosa Rorug02G0299300 Rorug04G0154500 Rorug04G0154600 Rorug04G0154700 Rorug06G0220500
rosa_samantha Rh1AG006300 Rh1AG139200 Rh1AG139300 Rh1BG105600 Rh1CG131200 Rh1CG131300 Rh1DG143200 Rh2AG210000 Rh2CG337500 Rh2DG216200 Rh2DG376200 Rh4AG215900 Rh4AG216000 Rh4AG216100 Rh4AG216200 Rh4AG216300 Rh4AG216400 Rh4BG214300 Rh4BG214400 Rh4BG214500 Rh4BG214600 Rh4BG214700 Rh4BG214800 Rh4CG212300 Rh4CG227500 Rh4CG227700 Rh4CG227900 Rh4CG228000 Rh4CG228100 Rh4DG214200 Rh4DG215000 Rh4DG215200 Rh5AG059400 Rh6AG332900 Rh6AG333000 Rh6AG333100 Rh6BG339700 Rh6BG339900 Rh6CG346100 Rh6CG346200 Rh6DG333300 Rh6DG333400 Rh7AG435400 Rh7CG454500
rosa_wichuraiana Rw1G011480 Rw2G028580 Rw4G018270 Rw4G018280 Rw4G018290 Rw4G018360 Rw4G018390 Rw4G018400 Rw4G018410 Rw4G018420 Rw6G028910 Rw6G029000 Rw6G029010

Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 182
AcoI YGGCCR 1 cut(s) 454
AcsI RAATTY 2 cut(s) 74, 247
AcuI CTGAAG 2 cut(s) 107, 693
AfaI GTAC 1 cut(s) 638
AflIII ACRYGT 1 cut(s) 690
AgsI TTSAA 1 cut(s) 547
AhdI GACNNNNNGTC 1 cut(s) 274
AjnI CCWGG 3 cut(s) 278, 451, 607
AluBI AGCT 8 cut(s) 17, 45, 121, 200, 290, 371, 512, 591
AluI AGCT 8 cut(s) 17, 45, 121, 200, 290, 371, 512, 591
Alw26I GTCTC 1 cut(s) 142
AoxI GGCC 2 cut(s) 82, 454
ApeKI GCWGC 6 cut(s) 14, 194, 197, 335, 371, 434
ApoI RAATTY 2 cut(s) 74, 247
AspS9I GGNCC 1 cut(s) 82
AsuHPI GGTGA 1 cut(s) 522
BbsI GAAGAC 1 cut(s) 95
BbvI GCAGC 6 cut(s) 26, 181, 209, 322, 358, 421
BccI CCATC 1 cut(s) 618
BciT130I CCWGG 3 cut(s) 280, 453, 609
BciVI GTATCC 1 cut(s) 302
BcoDI GTCTC 1 cut(s) 142
BfaI CTAG 2 cut(s) 71, 134
BfmI CTRYAG 4 cut(s) 12, 195, 372, 657
BfuI GTATCC 1 cut(s) 302
BisI GCNGC 6 cut(s) 15, 195, 198, 336, 372, 435
BlsI GCNGC 6 cut(s) 16, 196, 199, 337, 373, 436
Bme1390I CCNGG 3 cut(s) 280, 453, 609
BmeRI GACNNNNNGTC 1 cut(s) 274
BmgT120I GGNCC 1 cut(s) 82
BmiI GGNNCC 2 cut(s) 83, 219
BmrFI CCNGG 3 cut(s) 280, 453, 609
BmsI GCATC 1 cut(s) 457
BpiI GAAGAC 1 cut(s) 95
BpmI CTGGAG 1 cut(s) 630
BsaXI ACNNNNNCTCC 2 cut(s) 381, 411
Bse118I RCCGGY 1 cut(s) 456
Bse1I ACTGG 1 cut(s) 106
BseBI CCWGG 3 cut(s) 280, 453, 609
BseGI GGATG 3 cut(s) 448, 629, 655
BseMII CTCAG 2 cut(s) 504, 570
BseNI ACTGG 1 cut(s) 106
BseRI GAGGAG 1 cut(s) 572
BseXI GCAGC 6 cut(s) 26, 181, 209, 322, 358, 421
BseYI CCCAGC 1 cut(s) 384
Bsh1285I CGRYCG 1 cut(s) 271
BshFI GGCC 2 cut(s) 84, 456
BsiEI CGRYCG 1 cut(s) 271
BsiSI CCGG 1 cut(s) 457
BsmAI GTCTC 1 cut(s) 142
BsnI GGCC 2 cut(s) 84, 456
BspACI CCGC 1 cut(s) 182
BspANI GGCC 2 cut(s) 84, 456
BspCNI CTCAG 2 cut(s) 505, 571
BspLI GGNNCC 2 cut(s) 83, 219
BspMAI CTGCAG 4 cut(s) 16, 199, 376, 661
BsrFI RCCGGY 1 cut(s) 456
BsrI ACTGG 1 cut(s) 106
BssAI RCCGGY 1 cut(s) 456
Bst2UI CCWGG 3 cut(s) 280, 453, 609
Bst4CI ACNGT 1 cut(s) 67
Bst6I CTCTTC 1 cut(s) 669
BstAPI GCANNNNNTGC 1 cut(s) 410
BstC8I GCNNGC 2 cut(s) 19, 123
BstDEI CTNAG 4 cut(s) 41, 117, 513, 579
BstF5I GGATG 3 cut(s) 448, 629, 655
BstMAI GTCTC 1 cut(s) 142
BstMCI CGRYCG 1 cut(s) 271
BstMWI GCNNNNNNNGC 3 cut(s) 368, 410, 677
BstNI CCWGG 3 cut(s) 280, 453, 609
BstNSI RCATGY 1 cut(s) 694
BstSCI CCNGG 3 cut(s) 278, 451, 607
BstSFI CTRYAG 4 cut(s) 12, 195, 372, 657
BstV1I GCAGC 6 cut(s) 26, 181, 209, 322, 358, 421
BstV2I GAAGAC 1 cut(s) 95
BsuI GTATCC 1 cut(s) 302
BsuRI GGCC 2 cut(s) 84, 456
BtsCI GGATG 3 cut(s) 448, 629, 655
BtsIMutI CAGTG 1 cut(s) 576
Cac8I GCNNGC 2 cut(s) 19, 123
Cfr10I RCCGGY 1 cut(s) 456
Cfr13I GGNCC 1 cut(s) 82
Csp6I GTAC 1 cut(s) 637
CviAII CATG 3 cut(s) 479, 620, 691
CviQI GTAC 1 cut(s) 637
DdeI CTNAG 4 cut(s) 41, 117, 513, 579
DriI GACNNNNNGTC 1 cut(s) 274
EaeI YGGCCR 1 cut(s) 454
Eam1104I CTCTTC 1 cut(s) 669
Eam1105I GACNNNNNGTC 1 cut(s) 274
EarI CTCTTC 1 cut(s) 669
Eco57I CTGAAG 2 cut(s) 107, 693
EcoO109I RGGNCCY 1 cut(s) 82
EcoRII CCWGG 3 cut(s) 278, 451, 607
FaeI CATG 3 cut(s) 482, 623, 694
FatI CATG 3 cut(s) 478, 619, 690
Fnu4HI GCNGC 6 cut(s) 15, 195, 198, 336, 372, 435
FokI GGATG 3 cut(s) 435, 636, 662
Fsp4HI GCNGC 6 cut(s) 15, 195, 198, 336, 372, 435
FspBI CTAG 2 cut(s) 71, 134
GluI GCNGC 6 cut(s) 15, 195, 198, 336, 372, 435
GsaI CCCAGC 1 cut(s) 388
GsuI CTGGAG 1 cut(s) 630
HaeIII GGCC 2 cut(s) 84, 456
HapII CCGG 1 cut(s) 457
Hin1II CATG 3 cut(s) 482, 623, 694
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HindIII AAGCTT 1 cut(s) 119
HinfI GANTC 5 cut(s) 113, 275, 317, 346, 653
HpaII CCGG 1 cut(s) 457
HphI GGTGA 1 cut(s) 522
Hpy166II GTNNAC 2 cut(s) 6, 705
Hpy188I TCNGA 3 cut(s) 142, 421, 580
Hpy188III TCNNGA 3 cut(s) 25, 221, 664
Hpy8I GTNNAC 2 cut(s) 6, 705
HpyAV CCTTC 1 cut(s) 140
HpyCH4III ACNGT 1 cut(s) 67
HpyCH4IV ACGT 1 cut(s) 96
HpyF10VI GCNNNNNNNGC 3 cut(s) 368, 410, 677
HpyF3I CTNAG 4 cut(s) 41, 117, 513, 579
HpySE526I ACGT 1 cut(s) 96
Hsp92II CATG 3 cut(s) 482, 623, 694
LmnI GCTCC 2 cut(s) 368, 509
Lsp1109I GCAGC 6 cut(s) 26, 181, 209, 322, 358, 421
LweI GCATC 1 cut(s) 457
MaeI CTAG 2 cut(s) 71, 134
MaeII ACGT 1 cut(s) 96
MaeIII GTNAC 1 cut(s) 97
MboII GAAGA 3 cut(s) 100, 482, 686
MfeI CAATTG 1 cut(s) 542
MluCI AATT 6 cut(s) 74, 247, 263, 542, 551, 697
MlyI GAGTC 2 cut(s) 284, 355
MnlI CCTC 8 cut(s) 270, 307, 383, 475, 550, 627, 640, 670
MseI TTAA 5 cut(s) 284, 341, 429, 486, 695
MspA1I CMGCKG 1 cut(s) 200
MspI CCGG 1 cut(s) 457
MspR9I CCNGG 3 cut(s) 280, 453, 609
MunI CAATTG 1 cut(s) 542
MvaI CCWGG 3 cut(s) 280, 453, 609
MwoI GCNNNNNNNGC 3 cut(s) 368, 410, 677
NlaIII CATG 3 cut(s) 482, 623, 694
NlaIV GGNNCC 2 cut(s) 83, 219
NspI RCATGY 1 cut(s) 694
PciI ACATGT 1 cut(s) 690
PfeI GAWTC 3 cut(s) 113, 317, 653
PfoI TCCNGGA 1 cut(s) 607
PkrI GCNGC 6 cut(s) 16, 196, 199, 337, 373, 436
PleI GAGTC 2 cut(s) 283, 354
PpsI GAGTC 2 cut(s) 283, 354
PscI ACATGT 1 cut(s) 690
Psp6I CCWGG 3 cut(s) 278, 451, 607
PspFI CCCAGC 1 cut(s) 384
PspGI CCWGG 3 cut(s) 278, 451, 607
PspN4I GGNNCC 2 cut(s) 83, 219
PspPI GGNCC 1 cut(s) 82
PstI CTGCAG 4 cut(s) 16, 199, 376, 661
PvuII CAGCTG 1 cut(s) 200
RsaI GTAC 1 cut(s) 638
RsaNI GTAC 1 cut(s) 637
SaqAI TTAA 5 cut(s) 284, 341, 429, 486, 695
SatI GCNGC 6 cut(s) 15, 195, 198, 336, 372, 435
Sau96I GGNCC 1 cut(s) 82
SchI GAGTC 2 cut(s) 284, 355
ScrFI CCNGG 3 cut(s) 280, 453, 609
SfaNI GCATC 1 cut(s) 457
SfcI CTRYAG 4 cut(s) 12, 195, 372, 657
Sse9I AATT 6 cut(s) 74, 247, 263, 542, 551, 697
SsiI CCGC 1 cut(s) 182
SspI AATATT 2 cut(s) 504, 603
SspMI CTAG 2 cut(s) 71, 134
StyD4I CCNGG 3 cut(s) 278, 451, 607
TaaI ACNGT 1 cut(s) 67
TaiI ACGT 1 cut(s) 99
TaqI TCGA 2 cut(s) 24, 267
TaqII GACCGA 1 cut(s) 285
TasI AATT 6 cut(s) 74, 247, 263, 542, 551, 697
TfiI GAWTC 3 cut(s) 113, 317, 653
Tru1I TTAA 5 cut(s) 284, 341, 429, 486, 695
Tru9I TTAA 5 cut(s) 284, 341, 429, 486, 695
TscAI CASTG 1 cut(s) 576
TseI GCWGC 6 cut(s) 14, 194, 197, 335, 371, 434
TspDTI ATGAA 4 cut(s) 171, 482, 483, 666
TspGWI ACGGA 1 cut(s) 69
TspRI CASTG 1 cut(s) 576
XapI RAATTY 2 cut(s) 74, 247
XceI RCATGY 1 cut(s) 694
XspI CTAG 2 cut(s) 71, 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.