Rh4AG216300

1,4-beta-D-glucanase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000177
Physical Location & Seq
Forward (+)
51276007 .. 51278608
2602 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG216300.1

Sequence Viewer

Length: 480 bp
ATGTCAGGCCCTCAGTGCTGCTCGAACCCACCGATCCTGAACCCAACCAGTGGATCCGGCCACGTTGAGAAGCTTGGTGGTCTCGACTCCTATCTCACAGGCTCTCCTGAGGCCAAGCTTGCCATTGTTCTTGTCTCTGACGTTTTTGCCAAGGTTGTAGTTGAACTTGCAAAGGGTGACTTTATCCAAGCTGCTGTTCTGGCTCATCCTTCATTTGTCACTGTGGATGATATCAAGGCTGTTAAGGTTCCAATATCTATACTTGGAGCTGAGATTGATCGGATGTCTCCGCCTGAAGTTGTGAAACAATTTGAAGAGGCTTTGACTGCAAAGTCTGAGATCAAAAGTCATGTGAAGATATTCCCAAAAGTGTCACATGGATGGACTGTGAGGTATGATGTTTCAGACGAAGAAGCTGTGAAGCCTGCTGAGGAGGCTCATAAAGACTTGTTGGAGTGGTTCGTGAACCATGTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
ko00361 ko00364 ko00623 ko01100 ko01110 ko01120 ko01130 map00361 map00364 map00623 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
DLH

Protein Analysis

159

Amino Acids

17.2

Weight (kDa)

5.48

Isoelectric Point (pI)

35.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DLH PF01738 52 - 156 7.9e-11 Dienelactone hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000177)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23570 AT3G23570 AT3G23570 AT3G23600 AT3G23600
fragaria_vesca FvH4_2g23960 FvH4_2g23960 FvH4_2g23960 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14330 FvH4_6g28031 FvH4_6g29270
malus_domestica MD13G1181200.v1.1 MD13G1181300.v1.1 MD13G1181400.v1.1 MD13G1181500.v1.1 MD13G1181600.v1.1 MD13G1181700.v1.1 MD13G1181800.v1.1 MD13G1181900.v1.1 MD15G1403300.v1.1 MD15G1403400.v1.1 MD16G1181900.v1.1 MD16G1182000.v1.1 MD16G1182200.v1.1 MD16G1182300.v1.1 MD16G1182400.v1.1
prunus_persica Prupe.1G149400_v2.0.a1 Prupe.1G149600_v2.0.a1 Prupe.1G149700_v2.0.a1 Prupe.1G149800_v2.0.a1 Prupe.1G549400_v2.0.a1 Prupe.8G245600_v2.0.a1
pyrus_communis pycom13g15640 pycom13g15650 pycom13g15670 pycom13g15700 pycom15g36070 pycom16g15270 pycom16g15300 pycom16g15310
rosa_chinensis RchiOBHm_Chr1g0334461 RchiOBHm_Chr1g0334471 RchiOBHm_Chr2g0131731 RchiOBHm_Chr4g0417881 RchiOBHm_Chr4g0418561 RchiOBHm_Chr4g0418571 RchiOBHm_Chr4g0418581 RchiOBHm_Chr4g0418591 RchiOBHm_Chr4g0418601 RchiOBHm_Chr4g0418621 RchiOBHm_Chr4g0418651 RchiOBHm_Chr4g0418661 RchiOBHm_Chr4g0418671 RchiOBHm_Chr4g0418681 RchiOBHm_Chr4g0418691 RchiOBHm_Chr4g0418701 RchiOBHm_Chr4g0421371 RchiOBHm_Chr6g0291601 RchiOBHm_Chr6g0291611 RchiOBHm_Chr6g0291621 RchiOBHm_Chr6g0291631 RchiOBHm_Chr7g0229821
rosa_laevigata RLG00000007843 RLG00000007844 RLG00000007846 RLG00000007847 RLG00000007849 RLG00000012101 RLG00000012104 RLG00000029451
rosa_multiflora Rmu_sc0000363.1_g000035 Rmu_sc0000935.1_g000003 Rmu_sc0000935.1_g000010 Rmu_sc0002226.1_g000008 Rmu_sc0002226.1_g000014 Rmu_sc0002589.1_g000031 Rmu_sc0003443.1_g000009 Rmu_sc0006431.1_g000001 Rmu_sc0006431.1_g000006 Rmu_sc0006431.1_g000023 Rmu_sc0006705.1_g000014 Rmu_sc0006705.1_g000015 Rmu_sc0006705.1_g000016 Rmu_sc0006705.1_g000017 Rmu_sc0006705.1_g000018 Rmu_sc0014797.1_g000001 Rmu_sc0041885.1_g000001
rosa_roxburghii Rroxscaffold_4G00316820 Rroxscaffold_5G00361830 Rroxscaffold_5G00361840 Rroxscaffold_5G00361850 Rroxscaffold_5G00361860 Rroxscaffold_5G00361880 Rroxscaffold_5G00361890 Rroxscaffold_5G00361900 Rroxscaffold_5G00361910 Rroxscaffold_5G00361920 Rroxscaffold_5G00361930 Rroxscaffold_7G00175910 Rroxscaffold_7G00175930 Rroxscaffold_7G00175940
rosa_rugosa Rorug02G0299300 Rorug04G0154500 Rorug04G0154600 Rorug04G0154700 Rorug06G0220500
rosa_samantha Rh1AG006300 Rh1AG139200 Rh1AG139300 Rh1BG105600 Rh1CG131200 Rh1CG131300 Rh1DG143200 Rh2AG210000 Rh2CG337500 Rh2DG216200 Rh2DG376200 Rh4AG215900 Rh4AG216000 Rh4AG216100 Rh4AG216200 Rh4AG216300 Rh4AG216400 Rh4BG214300 Rh4BG214400 Rh4BG214500 Rh4BG214600 Rh4BG214700 Rh4BG214800 Rh4CG212300 Rh4CG227500 Rh4CG227700 Rh4CG227900 Rh4CG228000 Rh4CG228100 Rh4DG214200 Rh4DG215000 Rh4DG215200 Rh5AG059400 Rh6AG332900 Rh6AG333000 Rh6AG333100 Rh6BG339700 Rh6BG339900 Rh6CG346100 Rh6CG346200 Rh6DG333300 Rh6DG333400 Rh7AG435400 Rh7CG454500
rosa_wichuraiana Rw1G011480 Rw2G028580 Rw4G018270 Rw4G018280 Rw4G018290 Rw4G018360 Rw4G018390 Rw4G018400 Rw4G018410 Rw4G018420 Rw6G028910 Rw6G029000 Rw6G029010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 331
AccB7I CCANNNNNTGG 1 cut(s) 50
AciI CCGC 1 cut(s) 290
AclWI GGATC 3 cut(s) 28, 48, 61
AcoI YGGCCR 1 cut(s) 58
AcuI CTGAAG 1 cut(s) 315
AfiI CCNNNNNNNGG 1 cut(s) 50
AgsI TTSAA 2 cut(s) 164, 314
AjuI GAANNNNNNNTTGG 2 cut(s) 180, 212
AluBI AGCT 5 cut(s) 73, 118, 191, 269, 416
AluI AGCT 5 cut(s) 73, 118, 191, 269, 416
Alw26I GTCTC 3 cut(s) 86, 139, 291
AlwI GGATC 3 cut(s) 28, 48, 61
AoxI GGCC 3 cut(s) 7, 58, 111
ApeKI GCWGC 2 cut(s) 18, 191
Asp700I GAANNNNTTC 1 cut(s) 359
AspS9I GGNCC 1 cut(s) 8
AsuHPI GGTGA 1 cut(s) 188
AxyI CCTNAGG 1 cut(s) 108
BamHI GGATCC 1 cut(s) 53
BbvCI CCTCAGC 1 cut(s) 429
BbvI GCAGC 2 cut(s) 5, 178
BccI CCATC 1 cut(s) 375
BcoDI GTCTC 3 cut(s) 86, 139, 291
BisI GCNGC 2 cut(s) 19, 192
BlsI GCNGC 2 cut(s) 20, 193
BmgT120I GGNCC 1 cut(s) 8
BmiI GGNNCC 2 cut(s) 55, 249
Bpu10I CCTNAGC 1 cut(s) 429
BsaI GGTCTC 1 cut(s) 86
BsaJI CCNNGG 1 cut(s) 150
BsaXI ACNNNNNCTCC 2 cut(s) 88, 118
Bsc4I CCNNNNNNNGG 1 cut(s) 50
Bse1I ACTGG 1 cut(s) 48
Bse21I CCTNAGG 1 cut(s) 108
BseDI CCNNGG 1 cut(s) 150
BseGI GGATG 4 cut(s) 205, 232, 288, 386
BseLI CCNNNNNNNGG 1 cut(s) 50
BseMII CTCAG 5 cut(s) 26, 99, 261, 327, 420
BseNI ACTGG 1 cut(s) 48
BseRI GAGGAG 1 cut(s) 446
BseXI GCAGC 2 cut(s) 5, 178
BshFI GGCC 3 cut(s) 9, 60, 113
BsiSI CCGG 1 cut(s) 57
BslI CCNNNNNNNGG 1 cut(s) 50
BsmAI GTCTC 3 cut(s) 86, 139, 291
BsnI GGCC 3 cut(s) 9, 60, 113
Bso31I GGTCTC 1 cut(s) 86
Bsp143I GATC 4 cut(s) 33, 53, 277, 339
BspACI CCGC 1 cut(s) 290
BspANI GGCC 3 cut(s) 9, 60, 113
BspCNI CTCAG 5 cut(s) 25, 100, 262, 328, 421
BspLI GGNNCC 2 cut(s) 55, 249
BspPI GGATC 3 cut(s) 28, 48, 61
BspTNI GGTCTC 1 cut(s) 86
BsrI ACTGG 1 cut(s) 48
BssECI CCNNGG 1 cut(s) 150
BssMI GATC 4 cut(s) 33, 53, 277, 339
BssT1I CCWWGG 1 cut(s) 150
Bst4CI ACNGT 2 cut(s) 223, 388
Bst6I CTCTTC 1 cut(s) 309
BstC8I GCNNGC 2 cut(s) 120, 426
BstDEI CTNAG 5 cut(s) 12, 108, 270, 336, 429
BstF5I GGATG 4 cut(s) 205, 232, 288, 386
BstKTI GATC 4 cut(s) 36, 56, 280, 342
BstMAI GTCTC 3 cut(s) 86, 139, 291
BstMBI GATC 4 cut(s) 33, 53, 277, 339
BstMWI GCNNNNNNNGC 5 cut(s) 15, 119, 200, 326, 434
BstV1I GCAGC 2 cut(s) 5, 178
BstX2I RGATCY 1 cut(s) 53
BstYI RGATCY 1 cut(s) 53
Bsu36I CCTNAGG 1 cut(s) 108
BsuRI GGCC 3 cut(s) 9, 60, 113
BtsCI GGATG 4 cut(s) 205, 232, 288, 386
BtsIMutI CAGTG 3 cut(s) 20, 55, 219
Cac8I GCNNGC 2 cut(s) 120, 426
Cfr13I GGNCC 1 cut(s) 8
CviAII CATG 3 cut(s) 350, 377, 470
DdeI CTNAG 5 cut(s) 12, 108, 270, 336, 429
DpnI GATC 4 cut(s) 35, 55, 279, 341
DpnII GATC 4 cut(s) 33, 53, 277, 339
DrdI GACNNNNNNGTC 1 cut(s) 331
DseDI GACNNNNNNGTC 1 cut(s) 331
EaeI YGGCCR 1 cut(s) 58
Eam1104I CTCTTC 1 cut(s) 309
EarI CTCTTC 1 cut(s) 309
EciI GGCGGA 1 cut(s) 279
Eco130I CCWWGG 1 cut(s) 150
Eco31I GGTCTC 1 cut(s) 86
Eco32I GATATC 1 cut(s) 232
Eco57I CTGAAG 1 cut(s) 315
Eco81I CCTNAGG 1 cut(s) 108
EcoO109I RGGNCCY 1 cut(s) 8
EcoRV GATATC 1 cut(s) 232
EcoT14I CCWWGG 1 cut(s) 150
ErhI CCWWGG 1 cut(s) 150
FaeI CATG 3 cut(s) 353, 380, 473
FaiI YATR 6 cut(s) 260, 351, 378, 396, 441, 471
FalI AAGNNNNNCTT 2 cut(s) 164, 196
FatI CATG 3 cut(s) 349, 376, 469
Fnu4HI GCNGC 2 cut(s) 19, 192
FokI GGATG 4 cut(s) 192, 239, 295, 393
Fsp4HI GCNGC 2 cut(s) 19, 192
GluI GCNGC 2 cut(s) 19, 192
HaeIII GGCC 3 cut(s) 9, 60, 113
HapII CCGG 1 cut(s) 57
Hin1II CATG 3 cut(s) 353, 380, 473
HindIII AAGCTT 2 cut(s) 71, 116
HinfI GANTC 1 cut(s) 86
HpaII CCGG 1 cut(s) 57
HphI GGTGA 1 cut(s) 188
Hpy166II GTNNAC 1 cut(s) 466
Hpy188I TCNGA 4 cut(s) 139, 282, 337, 406
Hpy188III TCNNGA 4 cut(s) 37, 83, 107, 463
Hpy8I GTNNAC 1 cut(s) 466
HpyAV CCTTC 1 cut(s) 219
HpyCH4III ACNGT 2 cut(s) 223, 388
HpyCH4IV ACGT 2 cut(s) 63, 141
HpyCH4V TGCA 2 cut(s) 170, 329
HpyF10VI GCNNNNNNNGC 5 cut(s) 15, 119, 200, 326, 434
HpyF3I CTNAG 5 cut(s) 12, 108, 270, 336, 429
HpySE526I ACGT 2 cut(s) 63, 141
Hsp92II CATG 3 cut(s) 353, 380, 473
Kzo9I GATC 4 cut(s) 33, 53, 277, 339
LmnI GCTCC 1 cut(s) 266
LpnPI CCDG 8 cut(s) 50, 61, 70, 84, 120, 185, 306, 438
Lsp1109I GCAGC 2 cut(s) 5, 178
MaeII ACGT 2 cut(s) 63, 141
MaeIII GTNAC 3 cut(s) 176, 217, 372
MalI GATC 4 cut(s) 35, 55, 279, 341
MboI GATC 4 cut(s) 33, 53, 277, 339
MboII GAAGA 3 cut(s) 326, 367, 422
MflI RGATCY 1 cut(s) 53
MluCI AATT 1 cut(s) 308
MlyI GAGTC 1 cut(s) 80
MmeI TCCRAC 1 cut(s) 432
MnlI CCTC 6 cut(s) 21, 103, 310, 384, 424, 427
MroXI GAANNNNTTC 1 cut(s) 359
MseI TTAA 1 cut(s) 243
MslI CAYNNNNRTG 1 cut(s) 379
MspI CCGG 1 cut(s) 57
MwoI GCNNNNNNNGC 5 cut(s) 15, 119, 200, 326, 434
NdeII GATC 4 cut(s) 33, 53, 277, 339
NlaIII CATG 3 cut(s) 353, 380, 473
NlaIV GGNNCC 2 cut(s) 55, 249
NmuCI GTSAC 3 cut(s) 176, 217, 372
PcsI WCGNNNNNNNCGW 1 cut(s) 29
PdmI GAANNNNTTC 1 cut(s) 359
PflMI CCANNNNNTGG 1 cut(s) 50
PkrI GCNGC 2 cut(s) 20, 193
PleI GAGTC 1 cut(s) 80
PpsI GAGTC 1 cut(s) 80
PspN4I GGNNCC 2 cut(s) 55, 249
PspPI GGNCC 1 cut(s) 8
PsuI RGATCY 1 cut(s) 53
RseI CAYNNNNRTG 1 cut(s) 379
SaqAI TTAA 1 cut(s) 243
SatI GCNGC 2 cut(s) 19, 192
Sau3AI GATC 4 cut(s) 33, 53, 277, 339
Sau96I GGNCC 1 cut(s) 8
SchI GAGTC 1 cut(s) 80
SmiMI CAYNNNNRTG 1 cut(s) 379
Sse9I AATT 1 cut(s) 308
SsiI CCGC 1 cut(s) 290
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 2 cut(s) 223, 388
TaiI ACGT 2 cut(s) 66, 144
TaqI TCGA 2 cut(s) 23, 84
TasI AATT 1 cut(s) 308
Tru1I TTAA 1 cut(s) 243
Tru9I TTAA 1 cut(s) 243
TscAI CASTG 3 cut(s) 20, 55, 226
TseFI GTSAC 3 cut(s) 176, 217, 372
TseI GCWGC 2 cut(s) 18, 191
Tsp45I GTSAC 3 cut(s) 176, 217, 372
TspDTI ATGAA 1 cut(s) 201
TspRI CASTG 3 cut(s) 20, 55, 226
Van91I CCANNNNNTGG 1 cut(s) 50
XmnI GAANNNNTTC 1 cut(s) 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.