Rroxscaffold_5G00361830

1,4-beta-D-glucanase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000177
Physical Location & Seq
Forward (+)
42527141 .. 42530377
3237 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00361830.1

Sequence Viewer

Length: 726 bp
ATGTCAGGCCCTCAGTGCTGCTCGAACCCACCGACCCTGAACCCAACCAGTGGATCCGGCCACGTTGAGAAGCTTGGTGGTCTCGACTCCTATCTCACTGGCTCTCCTGACTCCAAGTTTGCCATTGTTCTTGTCTCCGACATTTATGGGTATGAGGCTCCAAACTTGAGGAAGCTCGCAGACAAAGTTGCAGCTGCTGGCTTCTTTGTTGTGGTCCCTGACTTCTTTTATGGACACCCTTACGTACCTGCAGCTGATGGATCCCGGGACTCTCTACCTACTTGGCTGAAAGGTCATGGACCGGAGAAGGGATTTGAAGATATTAAACTAGTTATTGGAGCTCTAAAAAGTAAAGGCGTCTCTGGAATCGGTGCTGCAGGCTTCTGTTGGGGAGCCAAGGTTGTAGTTGAACTTGCAAAGGGTGACTTTATCCAAGCTGCTGTTCTGGCTCATCCTTCTTTAGTCACTGTGGATGATATCAAGGCTGTTAAGGTTCCAATATCTATACTTGGAGCTGAGATTGATCAGATGTCTCCGCCTGAAGTCGTGAAACAATTTGAAGAGGCTTTGGCTGCAAAGTCTGAGATCAAAAGTCATGTGAAGATATTCCCAAAAGTGTCGCATGGATGGACTGTGAGGTATGATGTTTCAGACGAAGAAGCTGTGAAGCCCGCTGAGGAGGCTCATAAAGACTTGTTGGAGTGGTTCGTGAACCATGTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
ko00361 ko00364 ko00623 ko01100 ko01110 ko01120 ko01130 map00361 map00364 map00623 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
DLH

Protein Analysis

241

Amino Acids

25.77

Weight (kDa)

5.75

Isoelectric Point (pI)

27.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DLH PF01738 29 - 238 5e-30 Dienelactone hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000177)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23570 AT3G23570 AT3G23570 AT3G23600 AT3G23600
fragaria_vesca FvH4_2g23960 FvH4_2g23960 FvH4_2g23960 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14330 FvH4_6g28031 FvH4_6g29270
malus_domestica MD13G1181200.v1.1 MD13G1181300.v1.1 MD13G1181400.v1.1 MD13G1181500.v1.1 MD13G1181600.v1.1 MD13G1181700.v1.1 MD13G1181800.v1.1 MD13G1181900.v1.1 MD15G1403300.v1.1 MD15G1403400.v1.1 MD16G1181900.v1.1 MD16G1182000.v1.1 MD16G1182200.v1.1 MD16G1182300.v1.1 MD16G1182400.v1.1
prunus_persica Prupe.1G149400_v2.0.a1 Prupe.1G149600_v2.0.a1 Prupe.1G149700_v2.0.a1 Prupe.1G149800_v2.0.a1 Prupe.1G549400_v2.0.a1 Prupe.8G245600_v2.0.a1
pyrus_communis pycom13g15640 pycom13g15650 pycom13g15670 pycom13g15700 pycom15g36070 pycom16g15270 pycom16g15300 pycom16g15310
rosa_chinensis RchiOBHm_Chr1g0334461 RchiOBHm_Chr1g0334471 RchiOBHm_Chr2g0131731 RchiOBHm_Chr4g0417881 RchiOBHm_Chr4g0418561 RchiOBHm_Chr4g0418571 RchiOBHm_Chr4g0418581 RchiOBHm_Chr4g0418591 RchiOBHm_Chr4g0418601 RchiOBHm_Chr4g0418621 RchiOBHm_Chr4g0418651 RchiOBHm_Chr4g0418661 RchiOBHm_Chr4g0418671 RchiOBHm_Chr4g0418681 RchiOBHm_Chr4g0418691 RchiOBHm_Chr4g0418701 RchiOBHm_Chr4g0421371 RchiOBHm_Chr6g0291601 RchiOBHm_Chr6g0291611 RchiOBHm_Chr6g0291621 RchiOBHm_Chr6g0291631 RchiOBHm_Chr7g0229821
rosa_laevigata RLG00000007843 RLG00000007844 RLG00000007846 RLG00000007847 RLG00000007849 RLG00000012101 RLG00000012104 RLG00000029451
rosa_multiflora Rmu_sc0000363.1_g000035 Rmu_sc0000935.1_g000003 Rmu_sc0000935.1_g000010 Rmu_sc0002226.1_g000008 Rmu_sc0002226.1_g000014 Rmu_sc0002589.1_g000031 Rmu_sc0003443.1_g000009 Rmu_sc0006431.1_g000001 Rmu_sc0006431.1_g000006 Rmu_sc0006431.1_g000023 Rmu_sc0006705.1_g000014 Rmu_sc0006705.1_g000015 Rmu_sc0006705.1_g000016 Rmu_sc0006705.1_g000017 Rmu_sc0006705.1_g000018 Rmu_sc0014797.1_g000001 Rmu_sc0041885.1_g000001
rosa_roxburghii Rroxscaffold_4G00316820 Rroxscaffold_5G00361830 Rroxscaffold_5G00361840 Rroxscaffold_5G00361850 Rroxscaffold_5G00361860 Rroxscaffold_5G00361880 Rroxscaffold_5G00361890 Rroxscaffold_5G00361900 Rroxscaffold_5G00361910 Rroxscaffold_5G00361920 Rroxscaffold_5G00361930 Rroxscaffold_7G00175910 Rroxscaffold_7G00175930 Rroxscaffold_7G00175940
rosa_rugosa Rorug02G0299300 Rorug04G0154500 Rorug04G0154600 Rorug04G0154700 Rorug06G0220500
rosa_samantha Rh1AG006300 Rh1AG139200 Rh1AG139300 Rh1BG105600 Rh1CG131200 Rh1CG131300 Rh1DG143200 Rh2AG210000 Rh2CG337500 Rh2DG216200 Rh2DG376200 Rh4AG215900 Rh4AG216000 Rh4AG216100 Rh4AG216200 Rh4AG216300 Rh4AG216400 Rh4BG214300 Rh4BG214400 Rh4BG214500 Rh4BG214600 Rh4BG214700 Rh4BG214800 Rh4CG212300 Rh4CG227500 Rh4CG227700 Rh4CG227900 Rh4CG228000 Rh4CG228100 Rh4DG214200 Rh4DG215000 Rh4DG215200 Rh5AG059400 Rh6AG332900 Rh6AG333000 Rh6AG333100 Rh6BG339700 Rh6BG339900 Rh6CG346100 Rh6CG346200 Rh6DG333300 Rh6DG333400 Rh7AG435400 Rh7CG454500
rosa_wichuraiana Rw1G011480 Rw2G028580 Rw4G018270 Rw4G018280 Rw4G018290 Rw4G018360 Rw4G018390 Rw4G018400 Rw4G018410 Rw4G018420 Rw6G028910 Rw6G029000 Rw6G029010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 256
AccB7I CCANNNNNTGG 1 cut(s) 50
AciI CCGC 2 cut(s) 536, 672
AclWI GGATC 4 cut(s) 48, 61, 255, 268
AcoI YGGCCR 1 cut(s) 58
AcuI CTGAAG 1 cut(s) 561
AcyI GRCGYC 1 cut(s) 357
AfaI GTAC 1 cut(s) 246
AfiI CCNNNNNNNGG 2 cut(s) 50, 308
AgsI TTSAA 3 cut(s) 317, 410, 560
AhlI ACTAGT 1 cut(s) 328
AjuI GAANNNNNNNTTGG 2 cut(s) 426, 458
AluBI AGCT 8 cut(s) 73, 175, 194, 254, 341, 437, 515, 662
AluI AGCT 8 cut(s) 73, 175, 194, 254, 341, 437, 515, 662
Alw21I GWGCWC 1 cut(s) 343
Alw26I GTCTC 4 cut(s) 86, 139, 364, 537
AlwI GGATC 4 cut(s) 48, 61, 255, 268
AlwNI CAGNNNCTG 1 cut(s) 197
Ama87I CYCGRG 1 cut(s) 264
AoxI GGCC 2 cut(s) 7, 58
ApeKI GCWGC 7 cut(s) 18, 191, 194, 251, 374, 437, 572
ArsI GACNNNNNNTTYG 2 cut(s) 101, 133
Asp700I GAANNNNTTC 1 cut(s) 605
AspS9I GGNCC 3 cut(s) 8, 214, 299
AsuC2I CCSGG 2 cut(s) 265, 266
AsuHPI GGTGA 1 cut(s) 434
AvaI CYCGRG 1 cut(s) 264
AvaII GGWCC 2 cut(s) 214, 299
BamHI GGATCC 2 cut(s) 53, 260
BanII GRGCYC 1 cut(s) 343
Bbv12I GWGCWC 1 cut(s) 343
BbvCI CCTCAGC 1 cut(s) 675
BbvI GCAGC 7 cut(s) 5, 181, 203, 263, 361, 424, 559
BccI CCATC 2 cut(s) 251, 621
BclI TGATCA 1 cut(s) 523
BcnI CCSGG 2 cut(s) 265, 266
BcoDI GTCTC 4 cut(s) 86, 139, 364, 537
BcuI ACTAGT 1 cut(s) 328
BfaI CTAG 1 cut(s) 329
BfmI CTRYAG 2 cut(s) 249, 375
BfuAI ACCTGC 1 cut(s) 256
BisI GCNGC 7 cut(s) 19, 192, 195, 252, 375, 438, 573
BlsI GCNGC 7 cut(s) 20, 193, 196, 253, 376, 439, 574
Bme1390I CCNGG 2 cut(s) 265, 266
Bme18I GGWCC 2 cut(s) 214, 299
BmeT110I CYCGRG 1 cut(s) 264
BmgT120I GGNCC 3 cut(s) 8, 214, 299
BmiI GGNNCC 6 cut(s) 55, 159, 216, 262, 394, 495
BmrFI CCNGG 2 cut(s) 265, 266
Bpu10I CCTNAGC 1 cut(s) 675
BpuEI CTTGAG 1 cut(s) 187
BpuMI CCSGG 2 cut(s) 265, 266
BsaAI YACGTR 1 cut(s) 244
BsaHI GRCGYC 1 cut(s) 357
BsaI GGTCTC 1 cut(s) 86
BsaJI CCNNGG 2 cut(s) 264, 396
BsaWI WCCGGW 1 cut(s) 301
BsaXI ACNNNNNCTCC 4 cut(s) 88, 118, 384, 414
Bsc4I CCNNNNNNNGG 2 cut(s) 50, 308
Bse1I ACTGG 2 cut(s) 48, 103
BseDI CCNNGG 2 cut(s) 264, 396
BseGI GGATG 3 cut(s) 451, 478, 632
BseLI CCNNNNNNNGG 2 cut(s) 50, 308
BseMII CTCAG 4 cut(s) 26, 507, 573, 666
BseNI ACTGG 2 cut(s) 48, 103
BseRI GAGGAG 1 cut(s) 692
BseXI GCAGC 7 cut(s) 5, 181, 203, 263, 361, 424, 559
BshFI GGCC 2 cut(s) 9, 60
BsiHKAI GWGCWC 1 cut(s) 343
BsiHKCI CYCGRG 1 cut(s) 264
BsiSI CCGG 3 cut(s) 57, 265, 302
BslFI GGGAC 2 cut(s) 200, 281
BslI CCNNNNNNNGG 2 cut(s) 50, 308
BsmAI GTCTC 4 cut(s) 86, 139, 364, 537
BsmBI CGTCTC 1 cut(s) 364
BsmFI GGGAC 2 cut(s) 200, 281
BsnI GGCC 2 cut(s) 9, 60
Bso31I GGTCTC 1 cut(s) 86
BsoBI CYCGRG 1 cut(s) 264
Bsp1286I GDGCHC 1 cut(s) 343
Bsp143I GATC 4 cut(s) 53, 260, 523, 585
BspACI CCGC 2 cut(s) 536, 672
BspANI GGCC 2 cut(s) 9, 60
BspCNI CTCAG 4 cut(s) 25, 508, 574, 667
BspLI GGNNCC 6 cut(s) 55, 159, 216, 262, 394, 495
BspMAI CTGCAG 2 cut(s) 253, 379
BspMI ACCTGC 1 cut(s) 256
BspPI GGATC 4 cut(s) 48, 61, 255, 268
BspTNI GGTCTC 1 cut(s) 86
BsrI ACTGG 2 cut(s) 48, 103
BssECI CCNNGG 2 cut(s) 264, 396
BssMI GATC 4 cut(s) 53, 260, 523, 585
BssNI GRCGYC 1 cut(s) 357
BssT1I CCWWGG 1 cut(s) 396
Bst4CI ACNGT 2 cut(s) 469, 634
Bst6I CTCTTC 1 cut(s) 555
BstACI GRCGYC 1 cut(s) 357
BstBAI YACGTR 1 cut(s) 244
BstC8I GCNNGC 4 cut(s) 177, 199, 379, 672
BstDEI CTNAG 4 cut(s) 12, 516, 582, 675
BstF5I GGATG 3 cut(s) 451, 478, 632
BstKTI GATC 4 cut(s) 56, 263, 526, 588
BstMAI GTCTC 4 cut(s) 86, 139, 364, 537
BstMBI GATC 4 cut(s) 53, 260, 523, 585
BstMWI GCNNNNNNNGC 4 cut(s) 15, 446, 572, 680
BstSCI CCNGG 2 cut(s) 263, 264
BstSFI CTRYAG 2 cut(s) 249, 375
BstSNI TACGTA 1 cut(s) 244
BstV1I GCAGC 7 cut(s) 5, 181, 203, 263, 361, 424, 559
BstX2I RGATCY 2 cut(s) 53, 260
BstYI RGATCY 2 cut(s) 53, 260
BsuRI GGCC 2 cut(s) 9, 60
BtsCI GGATG 3 cut(s) 451, 478, 632
BtsIMutI CAGTG 4 cut(s) 20, 55, 96, 465
BveI ACCTGC 1 cut(s) 256
Cac8I GCNNGC 4 cut(s) 177, 199, 379, 672
CaiI CAGNNNCTG 1 cut(s) 197
Cfr13I GGNCC 3 cut(s) 8, 214, 299
Cfr9I CCCGGG 1 cut(s) 264
CseI GACGC 1 cut(s) 346
Csp6I GTAC 1 cut(s) 245
CviAII CATG 4 cut(s) 296, 596, 623, 716
CviQI GTAC 1 cut(s) 245
DdeI CTNAG 4 cut(s) 12, 516, 582, 675
DpnI GATC 4 cut(s) 55, 262, 525, 587
DpnII GATC 4 cut(s) 53, 260, 523, 585
EaeI YGGCCR 1 cut(s) 58
Eam1104I CTCTTC 1 cut(s) 555
EarI CTCTTC 1 cut(s) 555
EciI GGCGGA 1 cut(s) 525
Ecl136II GAGCTC 1 cut(s) 341
Eco105I TACGTA 1 cut(s) 244
Eco130I CCWWGG 1 cut(s) 396
Eco24I GRGCYC 1 cut(s) 343
Eco31I GGTCTC 1 cut(s) 86
Eco32I GATATC 1 cut(s) 478
Eco47I GGWCC 2 cut(s) 214, 299
Eco53kI GAGCTC 1 cut(s) 341
Eco57I CTGAAG 1 cut(s) 561
Eco88I CYCGRG 1 cut(s) 264
EcoICRI GAGCTC 1 cut(s) 341
EcoO109I RGGNCCY 1 cut(s) 8
EcoRV GATATC 1 cut(s) 478
EcoT14I CCWWGG 1 cut(s) 396
EcoT38I GRGCYC 1 cut(s) 343
ErhI CCWWGG 1 cut(s) 396
Esp3I CGTCTC 1 cut(s) 364
FaeI CATG 4 cut(s) 299, 599, 626, 719
FalI AAGNNNNNCTT 2 cut(s) 410, 442
FaqI GGGAC 2 cut(s) 200, 281
FatI CATG 4 cut(s) 295, 595, 622, 715
FauI CCCGC 1 cut(s) 679
FbaI TGATCA 1 cut(s) 523
Fnu4HI GCNGC 7 cut(s) 19, 192, 195, 252, 375, 438, 573
FokI GGATG 3 cut(s) 438, 485, 639
FriOI GRGCYC 1 cut(s) 343
Fsp4HI GCNGC 7 cut(s) 19, 192, 195, 252, 375, 438, 573
FspBI CTAG 1 cut(s) 329
GluI GCNGC 7 cut(s) 19, 192, 195, 252, 375, 438, 573
HaeIII GGCC 2 cut(s) 9, 60
HapII CCGG 3 cut(s) 57, 265, 302
HgaI GACGC 1 cut(s) 346
Hin1I GRCGYC 1 cut(s) 357
Hin1II CATG 4 cut(s) 299, 599, 626, 719
HindIII AAGCTT 1 cut(s) 71
HinfI GANTC 4 cut(s) 86, 110, 269, 366
HpaII CCGG 3 cut(s) 57, 265, 302
HphI GGTGA 1 cut(s) 434
Hpy166II GTNNAC 1 cut(s) 712
Hpy188I TCNGA 4 cut(s) 139, 528, 583, 652
Hpy188III TCNNGA 5 cut(s) 83, 107, 363, 547, 709
Hpy8I GTNNAC 1 cut(s) 712
HpyAV CCTTC 2 cut(s) 301, 465
HpyCH4III ACNGT 2 cut(s) 469, 634
HpyCH4IV ACGT 2 cut(s) 63, 243
HpyCH4V TGCA 5 cut(s) 191, 251, 377, 416, 575
HpyF10VI GCNNNNNNNGC 4 cut(s) 15, 446, 572, 680
HpyF3I CTNAG 4 cut(s) 12, 516, 582, 675
HpySE526I ACGT 2 cut(s) 63, 243
Hsp92I GRCGYC 1 cut(s) 357
Hsp92II CATG 4 cut(s) 299, 599, 626, 719
Ksp22I TGATCA 1 cut(s) 523
Kzo9I GATC 4 cut(s) 53, 260, 523, 585
LmnI GCTCC 4 cut(s) 163, 338, 392, 512
Lsp1109I GCAGC 7 cut(s) 5, 181, 203, 263, 361, 424, 559
MaeI CTAG 1 cut(s) 329
MaeII ACGT 2 cut(s) 63, 243
MaeIII GTNAC 2 cut(s) 422, 463
MalI GATC 4 cut(s) 55, 262, 525, 587
MboI GATC 4 cut(s) 53, 260, 523, 585
MboII GAAGA 4 cut(s) 329, 572, 613, 668
MflI RGATCY 2 cut(s) 53, 260
MhlI GDGCHC 1 cut(s) 343
MluCI AATT 1 cut(s) 554
MlyI GAGTC 3 cut(s) 80, 104, 263
MmeI TCCRAC 2 cut(s) 162, 678
MnlI CCTC 7 cut(s) 21, 148, 162, 556, 630, 670, 673
MroXI GAANNNNTTC 1 cut(s) 605
MseI TTAA 2 cut(s) 324, 489
MspA1I CMGCKG 3 cut(s) 194, 254, 674
MspI CCGG 3 cut(s) 57, 265, 302
MspR9I CCNGG 2 cut(s) 265, 266
MwoI GCNNNNNNNGC 4 cut(s) 15, 446, 572, 680
NciI CCSGG 2 cut(s) 265, 266
NdeII GATC 4 cut(s) 53, 260, 523, 585
NlaIII CATG 4 cut(s) 299, 599, 626, 719
NlaIV GGNNCC 6 cut(s) 55, 159, 216, 262, 394, 495
NmuCI GTSAC 2 cut(s) 422, 463
PcsI WCGNNNNNNNCGW 1 cut(s) 29
PdmI GAANNNNTTC 1 cut(s) 605
PfeI GAWTC 1 cut(s) 366
PflMI CCANNNNNTGG 1 cut(s) 50
PkrI GCNGC 7 cut(s) 20, 193, 196, 253, 376, 439, 574
PleI GAGTC 3 cut(s) 80, 104, 263
PpsI GAGTC 3 cut(s) 80, 104, 263
Ppu21I YACGTR 1 cut(s) 244
Psp124BI GAGCTC 1 cut(s) 343
PspN4I GGNNCC 6 cut(s) 55, 159, 216, 262, 394, 495
PspPI GGNCC 3 cut(s) 8, 214, 299
PstI CTGCAG 2 cut(s) 253, 379
PstNI CAGNNNCTG 1 cut(s) 197
PsuI RGATCY 2 cut(s) 53, 260
PvuII CAGCTG 2 cut(s) 194, 254
RsaI GTAC 1 cut(s) 246
RsaNI GTAC 1 cut(s) 245
SacI GAGCTC 1 cut(s) 343
SaqAI TTAA 2 cut(s) 324, 489
SatI GCNGC 7 cut(s) 19, 192, 195, 252, 375, 438, 573
Sau3AI GATC 4 cut(s) 53, 260, 523, 585
Sau96I GGNCC 3 cut(s) 8, 214, 299
SchI GAGTC 3 cut(s) 80, 104, 263
ScrFI CCNGG 2 cut(s) 265, 266
SduI GDGCHC 1 cut(s) 343
SfcI CTRYAG 2 cut(s) 249, 375
SinI GGWCC 2 cut(s) 214, 299
SmaI CCCGGG 1 cut(s) 266
SmlI CTYRAG 1 cut(s) 166
SmoI CTYRAG 1 cut(s) 166
SnaBI TACGTA 1 cut(s) 244
SpeI ACTAGT 1 cut(s) 328
Sse9I AATT 1 cut(s) 554
SsiI CCGC 2 cut(s) 536, 672
SspMI CTAG 1 cut(s) 329
SstI GAGCTC 1 cut(s) 343
StyD4I CCNGG 2 cut(s) 263, 264
StyI CCWWGG 1 cut(s) 396
TaaI ACNGT 2 cut(s) 469, 634
TaiI ACGT 2 cut(s) 66, 246
TaqI TCGA 2 cut(s) 23, 84
TasI AATT 1 cut(s) 554
TfiI GAWTC 1 cut(s) 366
Tru1I TTAA 2 cut(s) 324, 489
Tru9I TTAA 2 cut(s) 324, 489
TscAI CASTG 4 cut(s) 20, 55, 103, 472
TseFI GTSAC 2 cut(s) 422, 463
TseI GCWGC 7 cut(s) 18, 191, 194, 251, 374, 437, 572
Tsp45I GTSAC 2 cut(s) 422, 463
TspMI CCCGGG 1 cut(s) 264
TspRI CASTG 4 cut(s) 20, 55, 103, 472
Van91I CCANNNNNTGG 1 cut(s) 50
VpaK11BI GGWCC 2 cut(s) 214, 299
XmaI CCCGGG 1 cut(s) 264
XmnI GAANNNNTTC 1 cut(s) 605
XspI CTAG 1 cut(s) 329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.