Rh4CG227900

1,4-beta-D-glucanase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000177
Physical Location & Seq
Forward (+)
46516462 .. 46547034
30573 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG227900.1

Sequence Viewer

Length: 636 bp
ATGTCAGGCCCTCAGTGCTGCTCGAACCCACCGACCCTGAACCCAACCAGTGGATCCGGCCATGTTGAGAAGCTTGGTGGTCTCGACTCCTATCTCACTGGCTCTCCTGACTCCAAGCTTGCCATTGTTCTTCTCTCCGACATTTTTGGGTATGATGCTCCAAACTTGAGGAAGCTCGCAGACAAAGTTGCAGCTGCTGGCTTCTTTGTTGTGGTCCCTGACTTCTTTTATGGAGACCCTTATGTACTTGCAGCTGATGGATCCTTTGACTCTCTACCTACTTGGCTGAAAGGTCATGGACCGGAGAAGGGATTTGAAGATGTTAAATTAGTTATTGAAGCTCTAAAAAGTAAAGGCGTCTCTGCAATCGGTGCTGCAGGCTTATGTTGGGGAGCCAAGGTTGTAGTTGAACTTGCAAAGGGTGACTTTATCCAAGCTGCTGTTCTGGCTCATCCTTCATTTGTCACTGTGGATGATATCAAGGCCGGTTGGGATGGCCGCCTCTTCCAGTATGCTCTTTGCCTCACTCCACACTGGAGAGTTCTTGTTATATGGGTACGGGGGCCTGCATTTTATGACATGGAACTGGAATGTTATAAGGTAAGTGAAATTGCTGTCAATATCGATATTGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
ko00361 ko00364 ko00623 ko01100 ko01110 ko01120 ko01130 map00361 map00364 map00623 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
DLH

Protein Analysis

211

Amino Acids

22.64

Weight (kDa)

4.92

Isoelectric Point (pI)

28.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DLH PF01738 29 - 157 1.3e-16 Dienelactone hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000177)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23570 AT3G23570 AT3G23570 AT3G23600 AT3G23600
fragaria_vesca FvH4_2g23960 FvH4_2g23960 FvH4_2g23960 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14330 FvH4_6g28031 FvH4_6g29270
malus_domestica MD13G1181200.v1.1 MD13G1181300.v1.1 MD13G1181400.v1.1 MD13G1181500.v1.1 MD13G1181600.v1.1 MD13G1181700.v1.1 MD13G1181800.v1.1 MD13G1181900.v1.1 MD15G1403300.v1.1 MD15G1403400.v1.1 MD16G1181900.v1.1 MD16G1182000.v1.1 MD16G1182200.v1.1 MD16G1182300.v1.1 MD16G1182400.v1.1
prunus_persica Prupe.1G149400_v2.0.a1 Prupe.1G149600_v2.0.a1 Prupe.1G149700_v2.0.a1 Prupe.1G149800_v2.0.a1 Prupe.1G549400_v2.0.a1 Prupe.8G245600_v2.0.a1
pyrus_communis pycom13g15640 pycom13g15650 pycom13g15670 pycom13g15700 pycom15g36070 pycom16g15270 pycom16g15300 pycom16g15310
rosa_chinensis RchiOBHm_Chr1g0334461 RchiOBHm_Chr1g0334471 RchiOBHm_Chr2g0131731 RchiOBHm_Chr4g0417881 RchiOBHm_Chr4g0418561 RchiOBHm_Chr4g0418571 RchiOBHm_Chr4g0418581 RchiOBHm_Chr4g0418591 RchiOBHm_Chr4g0418601 RchiOBHm_Chr4g0418621 RchiOBHm_Chr4g0418651 RchiOBHm_Chr4g0418661 RchiOBHm_Chr4g0418671 RchiOBHm_Chr4g0418681 RchiOBHm_Chr4g0418691 RchiOBHm_Chr4g0418701 RchiOBHm_Chr4g0421371 RchiOBHm_Chr6g0291601 RchiOBHm_Chr6g0291611 RchiOBHm_Chr6g0291621 RchiOBHm_Chr6g0291631 RchiOBHm_Chr7g0229821
rosa_laevigata RLG00000007843 RLG00000007844 RLG00000007846 RLG00000007847 RLG00000007849 RLG00000012101 RLG00000012104 RLG00000029451
rosa_multiflora Rmu_sc0000363.1_g000035 Rmu_sc0000935.1_g000003 Rmu_sc0000935.1_g000010 Rmu_sc0002226.1_g000008 Rmu_sc0002226.1_g000014 Rmu_sc0002589.1_g000031 Rmu_sc0003443.1_g000009 Rmu_sc0006431.1_g000001 Rmu_sc0006431.1_g000006 Rmu_sc0006431.1_g000023 Rmu_sc0006705.1_g000014 Rmu_sc0006705.1_g000015 Rmu_sc0006705.1_g000016 Rmu_sc0006705.1_g000017 Rmu_sc0006705.1_g000018 Rmu_sc0014797.1_g000001 Rmu_sc0041885.1_g000001
rosa_roxburghii Rroxscaffold_4G00316820 Rroxscaffold_5G00361830 Rroxscaffold_5G00361840 Rroxscaffold_5G00361850 Rroxscaffold_5G00361860 Rroxscaffold_5G00361880 Rroxscaffold_5G00361890 Rroxscaffold_5G00361900 Rroxscaffold_5G00361910 Rroxscaffold_5G00361920 Rroxscaffold_5G00361930 Rroxscaffold_7G00175910 Rroxscaffold_7G00175930 Rroxscaffold_7G00175940
rosa_rugosa Rorug02G0299300 Rorug04G0154500 Rorug04G0154600 Rorug04G0154700 Rorug06G0220500
rosa_samantha Rh1AG006300 Rh1AG139200 Rh1AG139300 Rh1BG105600 Rh1CG131200 Rh1CG131300 Rh1DG143200 Rh2AG210000 Rh2CG337500 Rh2DG216200 Rh2DG376200 Rh4AG215900 Rh4AG216000 Rh4AG216100 Rh4AG216200 Rh4AG216300 Rh4AG216400 Rh4BG214300 Rh4BG214400 Rh4BG214500 Rh4BG214600 Rh4BG214700 Rh4BG214800 Rh4CG212300 Rh4CG227500 Rh4CG227700 Rh4CG227900 Rh4CG228000 Rh4CG228100 Rh4DG214200 Rh4DG215000 Rh4DG215200 Rh5AG059400 Rh6AG332900 Rh6AG333000 Rh6AG333100 Rh6BG339700 Rh6BG339900 Rh6CG346100 Rh6CG346200 Rh6DG333300 Rh6DG333400 Rh7AG435400 Rh7CG454500
rosa_wichuraiana Rw1G011480 Rw2G028580 Rw4G018270 Rw4G018280 Rw4G018290 Rw4G018360 Rw4G018390 Rw4G018400 Rw4G018410 Rw4G018420 Rw6G028910 Rw6G029000 Rw6G029010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 597
AccB7I CCANNNNNTGG 1 cut(s) 50
AciI CCGC 1 cut(s) 499
AclWI GGATC 4 cut(s) 48, 61, 255, 268
AcoI YGGCCR 2 cut(s) 58, 496
AcyI GRCGYC 1 cut(s) 357
AfaI GTAC 2 cut(s) 246, 558
AfiI CCNNNNNNNGG 2 cut(s) 50, 308
AgsI TTSAA 3 cut(s) 317, 338, 410
AjuI GAANNNNNNNTTGG 2 cut(s) 426, 458
AluBI AGCT 7 cut(s) 73, 118, 175, 194, 254, 341, 437
AluI AGCT 7 cut(s) 73, 118, 175, 194, 254, 341, 437
Alw26I GTCTC 3 cut(s) 86, 228, 364
AlwI GGATC 4 cut(s) 48, 61, 255, 268
AlwNI CAGNNNCTG 1 cut(s) 197
AoxI GGCC 5 cut(s) 7, 58, 483, 496, 563
ApeKI GCWGC 6 cut(s) 18, 191, 194, 251, 374, 437
AspS9I GGNCC 4 cut(s) 8, 214, 299, 563
AsuHPI GGTGA 1 cut(s) 434
AvaII GGWCC 2 cut(s) 214, 299
BamHI GGATCC 2 cut(s) 53, 260
BbvI GCAGC 6 cut(s) 5, 181, 203, 263, 361, 424
BccI CCATC 2 cut(s) 251, 488
BcoDI GTCTC 3 cut(s) 86, 228, 364
BfaI CTAG 1 cut(s) 634
BfmI CTRYAG 1 cut(s) 375
BisI GCNGC 7 cut(s) 19, 192, 195, 252, 375, 438, 499
BlsI GCNGC 7 cut(s) 20, 193, 196, 253, 376, 439, 500
Bme18I GGWCC 2 cut(s) 214, 299
BmgT120I GGNCC 4 cut(s) 8, 214, 299, 563
BmiI GGNNCC 5 cut(s) 55, 216, 262, 394, 564
BmsI GCATC 1 cut(s) 145
BpmI CTGGAG 1 cut(s) 556
BpuEI CTTGAG 1 cut(s) 187
Bsa29I ATCGAT 1 cut(s) 624
BsaHI GRCGYC 1 cut(s) 357
BsaI GGTCTC 2 cut(s) 86, 228
BsaJI CCNNGG 1 cut(s) 396
BsaWI WCCGGW 1 cut(s) 301
BsaXI ACNNNNNCTCC 4 cut(s) 88, 118, 384, 414
Bsc4I CCNNNNNNNGG 2 cut(s) 50, 308
Bse118I RCCGGY 1 cut(s) 485
Bse1I ACTGG 5 cut(s) 48, 103, 508, 539, 591
BseCI ATCGAT 1 cut(s) 624
BseDI CCNNGG 1 cut(s) 396
BseGI GGATG 3 cut(s) 451, 478, 499
BseLI CCNNNNNNNGG 2 cut(s) 50, 308
BseMII CTCAG 1 cut(s) 26
BseNI ACTGG 5 cut(s) 48, 103, 508, 539, 591
BseXI GCAGC 6 cut(s) 5, 181, 203, 263, 361, 424
BshFI GGCC 5 cut(s) 9, 60, 485, 498, 565
BshVI ATCGAT 1 cut(s) 624
BsiSI CCGG 3 cut(s) 57, 302, 486
BslFI GGGAC 1 cut(s) 200
BslI CCNNNNNNNGG 2 cut(s) 50, 308
BsmAI GTCTC 3 cut(s) 86, 228, 364
BsmBI CGTCTC 1 cut(s) 364
BsmFI GGGAC 1 cut(s) 200
BsnI GGCC 5 cut(s) 9, 60, 485, 498, 565
Bso31I GGTCTC 2 cut(s) 86, 228
Bsp143I GATC 2 cut(s) 53, 260
BspACI CCGC 1 cut(s) 499
BspANI GGCC 5 cut(s) 9, 60, 485, 498, 565
BspCNI CTCAG 1 cut(s) 25
BspDI ATCGAT 1 cut(s) 624
BspLI GGNNCC 5 cut(s) 55, 216, 262, 394, 564
BspMAI CTGCAG 1 cut(s) 379
BspPI GGATC 4 cut(s) 48, 61, 255, 268
BspTNI GGTCTC 2 cut(s) 86, 228
BsrFI RCCGGY 1 cut(s) 485
BsrI ACTGG 5 cut(s) 48, 103, 508, 539, 591
BssAI RCCGGY 1 cut(s) 485
BssECI CCNNGG 1 cut(s) 396
BssMI GATC 2 cut(s) 53, 260
BssNI GRCGYC 1 cut(s) 357
BssT1I CCWWGG 1 cut(s) 396
Bst4CI ACNGT 1 cut(s) 469
Bst6I CTCTTC 1 cut(s) 509
BstACI GRCGYC 1 cut(s) 357
BstAPI GCANNNNNTGC 1 cut(s) 371
BstC8I GCNNGC 5 cut(s) 120, 177, 199, 379, 567
BstDEI CTNAG 1 cut(s) 12
BstF5I GGATG 3 cut(s) 451, 478, 499
BstKTI GATC 2 cut(s) 56, 263
BstMAI GTCTC 3 cut(s) 86, 228, 364
BstMBI GATC 2 cut(s) 53, 260
BstMWI GCNNNNNNNGC 3 cut(s) 15, 371, 446
BstSFI CTRYAG 1 cut(s) 375
BstV1I GCAGC 6 cut(s) 5, 181, 203, 263, 361, 424
BstX2I RGATCY 2 cut(s) 53, 260
BstYI RGATCY 2 cut(s) 53, 260
Bsu15I ATCGAT 1 cut(s) 624
BsuRI GGCC 5 cut(s) 9, 60, 485, 498, 565
BsuTUI ATCGAT 1 cut(s) 624
BtsCI GGATG 3 cut(s) 451, 478, 499
BtsIMutI CAGTG 5 cut(s) 20, 55, 96, 465, 532
Cac8I GCNNGC 5 cut(s) 120, 177, 199, 379, 567
CaiI CAGNNNCTG 1 cut(s) 197
Cfr10I RCCGGY 1 cut(s) 485
Cfr13I GGNCC 4 cut(s) 8, 214, 299, 563
ClaI ATCGAT 1 cut(s) 624
CseI GACGC 1 cut(s) 346
Csp6I GTAC 2 cut(s) 245, 557
CviAII CATG 3 cut(s) 62, 296, 580
CviQI GTAC 2 cut(s) 245, 557
DdeI CTNAG 1 cut(s) 12
DpnI GATC 2 cut(s) 55, 262
DpnII GATC 2 cut(s) 53, 260
EaeI YGGCCR 2 cut(s) 58, 496
Eam1104I CTCTTC 1 cut(s) 509
EarI CTCTTC 1 cut(s) 509
Eco130I CCWWGG 1 cut(s) 396
Eco31I GGTCTC 2 cut(s) 86, 228
Eco32I GATATC 1 cut(s) 478
Eco47I GGWCC 2 cut(s) 214, 299
EcoO109I RGGNCCY 2 cut(s) 8, 563
EcoRV GATATC 1 cut(s) 478
EcoT14I CCWWGG 1 cut(s) 396
ErhI CCWWGG 1 cut(s) 396
Esp3I CGTCTC 1 cut(s) 364
FaeI CATG 3 cut(s) 65, 299, 583
FalI AAGNNNNNCTT 2 cut(s) 410, 442
FaqI GGGAC 1 cut(s) 200
FatI CATG 3 cut(s) 61, 295, 579
Fnu4HI GCNGC 7 cut(s) 19, 192, 195, 252, 375, 438, 499
FokI GGATG 3 cut(s) 438, 485, 506
Fsp4HI GCNGC 7 cut(s) 19, 192, 195, 252, 375, 438, 499
FspBI CTAG 1 cut(s) 634
GluI GCNGC 7 cut(s) 19, 192, 195, 252, 375, 438, 499
GsuI CTGGAG 1 cut(s) 556
HaeIII GGCC 5 cut(s) 9, 60, 485, 498, 565
HapII CCGG 3 cut(s) 57, 302, 486
HgaI GACGC 1 cut(s) 346
Hin1I GRCGYC 1 cut(s) 357
Hin1II CATG 3 cut(s) 65, 299, 583
HindIII AAGCTT 2 cut(s) 71, 116
HinfI GANTC 3 cut(s) 86, 110, 269
HpaII CCGG 3 cut(s) 57, 302, 486
HphI GGTGA 1 cut(s) 434
Hpy188I TCNGA 1 cut(s) 139
Hpy188III TCNNGA 2 cut(s) 83, 107
HpyAV CCTTC 2 cut(s) 301, 465
HpyCH4III ACNGT 1 cut(s) 469
HpyCH4V TGCA 6 cut(s) 191, 251, 365, 377, 416, 569
HpyF10VI GCNNNNNNNGC 3 cut(s) 15, 371, 446
HpyF3I CTNAG 1 cut(s) 12
Hsp92I GRCGYC 1 cut(s) 357
Hsp92II CATG 3 cut(s) 65, 299, 583
Kzo9I GATC 2 cut(s) 53, 260
LmnI GCTCC 2 cut(s) 163, 392
Lsp1109I GCAGC 6 cut(s) 5, 181, 203, 263, 361, 424
LweI GCATC 1 cut(s) 145
MaeI CTAG 1 cut(s) 634
MaeIII GTNAC 2 cut(s) 422, 463
MalI GATC 2 cut(s) 55, 262
MboI GATC 2 cut(s) 53, 260
MboII GAAGA 3 cut(s) 122, 329, 496
MflI RGATCY 2 cut(s) 53, 260
MluCI AATT 2 cut(s) 326, 609
MlyI GAGTC 3 cut(s) 80, 104, 263
MmeI TCCRAC 1 cut(s) 162
MnlI CCTC 4 cut(s) 21, 162, 512, 533
MseI TTAA 1 cut(s) 324
MspA1I CMGCKG 2 cut(s) 194, 254
MspI CCGG 3 cut(s) 57, 302, 486
MwoI GCNNNNNNNGC 3 cut(s) 15, 371, 446
NdeII GATC 2 cut(s) 53, 260
NlaIII CATG 3 cut(s) 65, 299, 583
NlaIV GGNNCC 5 cut(s) 55, 216, 262, 394, 564
NmuCI GTSAC 2 cut(s) 422, 463
PcsI WCGNNNNNNNCGW 1 cut(s) 29
PflMI CCANNNNNTGG 1 cut(s) 50
PkrI GCNGC 7 cut(s) 20, 193, 196, 253, 376, 439, 500
PleI GAGTC 3 cut(s) 80, 104, 263
PpsI GAGTC 3 cut(s) 80, 104, 263
PsiI TTATAA 1 cut(s) 597
PspN4I GGNNCC 5 cut(s) 55, 216, 262, 394, 564
PspPI GGNCC 4 cut(s) 8, 214, 299, 563
PstI CTGCAG 1 cut(s) 379
PstNI CAGNNNCTG 1 cut(s) 197
PsuI RGATCY 2 cut(s) 53, 260
PvuII CAGCTG 2 cut(s) 194, 254
RsaI GTAC 2 cut(s) 246, 558
RsaNI GTAC 2 cut(s) 245, 557
SaqAI TTAA 1 cut(s) 324
SatI GCNGC 7 cut(s) 19, 192, 195, 252, 375, 438, 499
Sau3AI GATC 2 cut(s) 53, 260
Sau96I GGNCC 4 cut(s) 8, 214, 299, 563
SchI GAGTC 3 cut(s) 80, 104, 263
SfaNI GCATC 1 cut(s) 145
SfcI CTRYAG 1 cut(s) 375
SinI GGWCC 2 cut(s) 214, 299
SmlI CTYRAG 1 cut(s) 166
SmoI CTYRAG 1 cut(s) 166
Sse9I AATT 2 cut(s) 326, 609
SsiI CCGC 1 cut(s) 499
SspMI CTAG 1 cut(s) 634
StyI CCWWGG 1 cut(s) 396
TaaI ACNGT 1 cut(s) 469
TaqI TCGA 3 cut(s) 23, 84, 624
TasI AATT 2 cut(s) 326, 609
TatI WGTACW 1 cut(s) 244
TauI GCSGC 1 cut(s) 501
Tru1I TTAA 1 cut(s) 324
Tru9I TTAA 1 cut(s) 324
TscAI CASTG 5 cut(s) 20, 55, 103, 472, 539
TseFI GTSAC 2 cut(s) 422, 463
TseI GCWGC 6 cut(s) 18, 191, 194, 251, 374, 437
Tsp45I GTSAC 2 cut(s) 422, 463
TspDTI ATGAA 1 cut(s) 447
TspRI CASTG 5 cut(s) 20, 55, 103, 472, 539
Van91I CCANNNNNTGG 1 cut(s) 50
VpaK11BI GGWCC 2 cut(s) 214, 299
XspI CTAG 1 cut(s) 634
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.