MD13G1181900.v1.1

hydrolase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0000177
Physical Location & Seq
Reverse (-)
15210953 .. 15213358
2406 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1181900.v1.1.491

Sequence Viewer

Length: 726 bp
ATGGCAGCTAACAGTGAATGCTTCAACAACCCGCCGCCACTGAGCTCAACCTCCGGACCTGGGACTCTCCTACAACTTGGGGGCCTTACGACCTACGTCGCTGGCCCATCTGAATCCAAGCTTGCAATCCTTCTAATCTCCGATATTTGGGGCTATGAAAACCTAAATCTGAGGAAAATAGCGGATAAAGTTGCTGCAGCTGGATTTTTGGCGGTAGTTCCTGATTTCTTTTACGGCGACCCTGTTAATCCCGCTAACCCGCAGTTCGATGCAGATATATGGTTCAAAATTCATAGCACGGAGAAAGGATGCGAAGATGCAAAACCGTTGATTGCTGCTCTGAAGAGTCAAGGCGTTTCCGCCATCGGAGCTGCAGGCTTTTGCTGGGGAGGGAATGTGGCGGTGAAACTAGGGAAAACCGATGAGATTCAGGCCGCTGTAATACTGCATCCTGGTCGATTAACAGATGAGGAAATCGATCAGGTTAAGGTTCCTACTGCAATATTGGGAGCTGAGACTGACAAGCATTCACCCGCTGAACAAATGAAACACTTTGGGGATATTTTATCAACTAAATCTGAGCTGTTTGATAGCTTTGTGAAAATATATCCTGGAGCGGCTCATGGATGGACAACAAAGTACAATTCTGAGGATGAATTGGCAGTCAAGAGAGCTGACGAGGCTCATTTGGACATGTTAAATTGGCTTACCAAGTATGTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
ko00361 ko00364 ko00623 ko01100 ko01110 ko01120 ko01130 map00361 map00364 map00623 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
DLH

Protein Analysis

242

Amino Acids

25.95

Weight (kDa)

5.12

Isoelectric Point (pI)

32.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DLH PF01738 30 - 239 1.1e-36 Dienelactone hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000177)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23570 AT3G23570 AT3G23570 AT3G23600 AT3G23600
fragaria_vesca FvH4_2g23960 FvH4_2g23960 FvH4_2g23960 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14330 FvH4_6g28031 FvH4_6g29270
malus_domestica MD13G1181200.v1.1 MD13G1181300.v1.1 MD13G1181400.v1.1 MD13G1181500.v1.1 MD13G1181600.v1.1 MD13G1181700.v1.1 MD13G1181800.v1.1 MD13G1181900.v1.1 MD15G1403300.v1.1 MD15G1403400.v1.1 MD16G1181900.v1.1 MD16G1182000.v1.1 MD16G1182200.v1.1 MD16G1182300.v1.1 MD16G1182400.v1.1
prunus_persica Prupe.1G149400_v2.0.a1 Prupe.1G149600_v2.0.a1 Prupe.1G149700_v2.0.a1 Prupe.1G149800_v2.0.a1 Prupe.1G549400_v2.0.a1 Prupe.8G245600_v2.0.a1
pyrus_communis pycom13g15640 pycom13g15650 pycom13g15670 pycom13g15700 pycom15g36070 pycom16g15270 pycom16g15300 pycom16g15310
rosa_chinensis RchiOBHm_Chr1g0334461 RchiOBHm_Chr1g0334471 RchiOBHm_Chr2g0131731 RchiOBHm_Chr4g0417881 RchiOBHm_Chr4g0418561 RchiOBHm_Chr4g0418571 RchiOBHm_Chr4g0418581 RchiOBHm_Chr4g0418591 RchiOBHm_Chr4g0418601 RchiOBHm_Chr4g0418621 RchiOBHm_Chr4g0418651 RchiOBHm_Chr4g0418661 RchiOBHm_Chr4g0418671 RchiOBHm_Chr4g0418681 RchiOBHm_Chr4g0418691 RchiOBHm_Chr4g0418701 RchiOBHm_Chr4g0421371 RchiOBHm_Chr6g0291601 RchiOBHm_Chr6g0291611 RchiOBHm_Chr6g0291621 RchiOBHm_Chr6g0291631 RchiOBHm_Chr7g0229821
rosa_laevigata RLG00000007843 RLG00000007844 RLG00000007846 RLG00000007847 RLG00000007849 RLG00000012101 RLG00000012104 RLG00000029451
rosa_multiflora Rmu_sc0000363.1_g000035 Rmu_sc0000935.1_g000003 Rmu_sc0000935.1_g000010 Rmu_sc0002226.1_g000008 Rmu_sc0002226.1_g000014 Rmu_sc0002589.1_g000031 Rmu_sc0003443.1_g000009 Rmu_sc0006431.1_g000001 Rmu_sc0006431.1_g000006 Rmu_sc0006431.1_g000023 Rmu_sc0006705.1_g000014 Rmu_sc0006705.1_g000015 Rmu_sc0006705.1_g000016 Rmu_sc0006705.1_g000017 Rmu_sc0006705.1_g000018 Rmu_sc0014797.1_g000001 Rmu_sc0041885.1_g000001
rosa_roxburghii Rroxscaffold_4G00316820 Rroxscaffold_5G00361830 Rroxscaffold_5G00361840 Rroxscaffold_5G00361850 Rroxscaffold_5G00361860 Rroxscaffold_5G00361880 Rroxscaffold_5G00361890 Rroxscaffold_5G00361900 Rroxscaffold_5G00361910 Rroxscaffold_5G00361920 Rroxscaffold_5G00361930 Rroxscaffold_7G00175910 Rroxscaffold_7G00175930 Rroxscaffold_7G00175940
rosa_rugosa Rorug02G0299300 Rorug04G0154500 Rorug04G0154600 Rorug04G0154700 Rorug06G0220500
rosa_samantha Rh1AG006300 Rh1AG139200 Rh1AG139300 Rh1BG105600 Rh1CG131200 Rh1CG131300 Rh1DG143200 Rh2AG210000 Rh2CG337500 Rh2DG216200 Rh2DG376200 Rh4AG215900 Rh4AG216000 Rh4AG216100 Rh4AG216200 Rh4AG216300 Rh4AG216400 Rh4BG214300 Rh4BG214400 Rh4BG214500 Rh4BG214600 Rh4BG214700 Rh4BG214800 Rh4CG212300 Rh4CG227500 Rh4CG227700 Rh4CG227900 Rh4CG228000 Rh4CG228100 Rh4DG214200 Rh4DG215000 Rh4DG215200 Rh5AG059400 Rh6AG332900 Rh6AG333000 Rh6AG333100 Rh6BG339700 Rh6BG339900 Rh6CG346100 Rh6CG346200 Rh6DG333300 Rh6DG333400 Rh7AG435400 Rh7CG454500
rosa_wichuraiana Rw1G011480 Rw2G028580 Rw4G018270 Rw4G018280 Rw4G018290 Rw4G018360 Rw4G018390 Rw4G018400 Rw4G018410 Rw4G018420 Rw6G028910 Rw6G029000 Rw6G029010

Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 617
AccIII TCCGGA 1 cut(s) 53
AcsI RAATTY 1 cut(s) 288
AcuI CTGAAG 1 cut(s) 362
AfaI GTAC 1 cut(s) 641
AfiI CCNNNNNNNGG 2 cut(s) 60, 147
AflIII ACRYGT 1 cut(s) 693
AgsI TTSAA 2 cut(s) 25, 286
AjnI CCWGG 3 cut(s) 58, 451, 610
AluBI AGCT 9 cut(s) 8, 45, 121, 200, 371, 512, 583, 594, 674
AluI AGCT 9 cut(s) 8, 45, 121, 200, 371, 512, 583, 594, 674
Alw21I GWGCWC 1 cut(s) 47
Alw26I GTCTC 1 cut(s) 509
Aor13HI TCCGGA 1 cut(s) 53
AoxI GGCC 3 cut(s) 82, 103, 432
ApeKI GCWGC 5 cut(s) 5, 194, 197, 335, 371
ApoI RAATTY 1 cut(s) 288
AspS9I GGNCC 3 cut(s) 56, 82, 104
AsuHPI GGTGA 2 cut(s) 415, 522
AvaII GGWCC 1 cut(s) 56
BanII GRGCYC 1 cut(s) 47
Bbv12I GWGCWC 1 cut(s) 47
BbvI GCAGC 5 cut(s) 17, 181, 209, 322, 358
BccI CCATC 3 cut(s) 115, 371, 621
BceAI ACGGC 1 cut(s) 250
BcgI CGANNNNNNTGC 2 cut(s) 437, 471
BciT130I CCWGG 3 cut(s) 60, 453, 612
BcoDI GTCTC 1 cut(s) 509
BfaI CTAG 1 cut(s) 410
BfmI CTRYAG 2 cut(s) 195, 372
BisI GCNGC 8 cut(s) 6, 35, 195, 198, 336, 372, 435, 618
BlsI GCNGC 8 cut(s) 7, 36, 196, 199, 337, 373, 436, 619
Bme1390I CCNGG 3 cut(s) 60, 453, 612
Bme18I GGWCC 1 cut(s) 56
BmgT120I GGNCC 3 cut(s) 56, 82, 104
BmiI GGNNCC 2 cut(s) 83, 492
BmrFI CCNGG 3 cut(s) 60, 453, 612
BmsI GCATC 4 cut(s) 259, 299, 307, 457
BoxI GACNNNNGTC 1 cut(s) 95
BpmI CTGGAG 1 cut(s) 633
Bsa29I ATCGAT 1 cut(s) 477
BsaJI CCNNGG 1 cut(s) 59
BsaWI WCCGGW 1 cut(s) 53
BsaXI ACNNNNNCTCC 2 cut(s) 381, 411
Bsc4I CCNNNNNNNGG 2 cut(s) 60, 147
BseAI TCCGGA 1 cut(s) 53
BseBI CCWGG 3 cut(s) 60, 453, 612
BseCI ATCGAT 1 cut(s) 477
BseDI CCNNGG 1 cut(s) 59
BseGI GGATG 4 cut(s) 314, 448, 632, 658
BseLI CCNNNNNNNGG 2 cut(s) 60, 147
BseMII CTCAG 5 cut(s) 32, 161, 504, 570, 639
BseXI GCAGC 5 cut(s) 17, 181, 209, 322, 358
BseYI CCCAGC 1 cut(s) 384
BshFI GGCC 3 cut(s) 84, 105, 434
BshVI ATCGAT 1 cut(s) 477
BsiHKAI GWGCWC 1 cut(s) 47
BsiSI CCGG 1 cut(s) 54
BslFI GGGAC 1 cut(s) 76
BslI CCNNNNNNNGG 2 cut(s) 60, 147
BsmAI GTCTC 1 cut(s) 509
BsmFI GGGAC 1 cut(s) 76
BsmI GAATGC 2 cut(s) 23, 526
BsnI GGCC 3 cut(s) 84, 105, 434
Bsp1286I GDGCHC 1 cut(s) 47
Bsp13I TCCGGA 1 cut(s) 53
Bsp143I GATC 1 cut(s) 478
BspANI GGCC 3 cut(s) 84, 105, 434
BspCNI CTCAG 5 cut(s) 33, 162, 505, 571, 640
BspDI ATCGAT 1 cut(s) 477
BspEI TCCGGA 1 cut(s) 53
BspLI GGNNCC 2 cut(s) 83, 492
BspMAI CTGCAG 2 cut(s) 199, 376
BsrBI CCGCTC 1 cut(s) 617
BssECI CCNNGG 1 cut(s) 59
BssMI GATC 1 cut(s) 478
Bst2UI CCWGG 3 cut(s) 60, 453, 612
Bst4CI ACNGT 2 cut(s) 14, 327
Bst6I CTCTTC 1 cut(s) 338
BstC8I GCNNGC 3 cut(s) 103, 123, 376
BstDEI CTNAG 5 cut(s) 41, 170, 513, 579, 648
BstF5I GGATG 4 cut(s) 314, 448, 632, 658
BstKTI GATC 1 cut(s) 481
BstMAI GTCTC 1 cut(s) 509
BstMBI GATC 1 cut(s) 478
BstMWI GCNNNNNNNGC 2 cut(s) 368, 680
BstNI CCWGG 3 cut(s) 60, 453, 612
BstNSI RCATGY 1 cut(s) 697
BstPAI GACNNNNGTC 1 cut(s) 95
BstSCI CCNGG 3 cut(s) 58, 451, 610
BstSFI CTRYAG 2 cut(s) 195, 372
BstV1I GCAGC 5 cut(s) 17, 181, 209, 322, 358
Bsu15I ATCGAT 1 cut(s) 477
BsuRI GGCC 3 cut(s) 84, 105, 434
BsuTUI ATCGAT 1 cut(s) 477
BtsCI GGATG 4 cut(s) 314, 448, 632, 658
BtsIMutI CAGTG 2 cut(s) 19, 38
Cac8I GCNNGC 3 cut(s) 103, 123, 376
Cfr13I GGNCC 3 cut(s) 56, 82, 104
ClaI ATCGAT 1 cut(s) 477
Csp6I GTAC 1 cut(s) 640
CviAII CATG 2 cut(s) 623, 694
CviQI GTAC 1 cut(s) 640
DdeI CTNAG 5 cut(s) 41, 170, 513, 579, 648
DpnI GATC 1 cut(s) 480
DpnII GATC 1 cut(s) 478
Eam1104I CTCTTC 1 cut(s) 338
EarI CTCTTC 1 cut(s) 338
EciI GGCGGA 1 cut(s) 349
Ecl136II GAGCTC 1 cut(s) 45
Eco24I GRGCYC 1 cut(s) 47
Eco47I GGWCC 1 cut(s) 56
Eco53kI GAGCTC 1 cut(s) 45
Eco57I CTGAAG 1 cut(s) 362
EcoICRI GAGCTC 1 cut(s) 45
EcoO109I RGGNCCY 1 cut(s) 82
EcoRII CCWGG 3 cut(s) 58, 451, 610
EcoT38I GRGCYC 1 cut(s) 47
FaeI CATG 2 cut(s) 626, 697
FaiI YATR 8 cut(s) 156, 278, 280, 294, 607, 624, 695, 717
FaqI GGGAC 1 cut(s) 76
FatI CATG 2 cut(s) 622, 693
FauI CCCGC 4 cut(s) 39, 259, 267, 541
Fnu4HI GCNGC 8 cut(s) 6, 35, 195, 198, 336, 372, 435, 618
FokI GGATG 4 cut(s) 321, 435, 639, 665
FriOI GRGCYC 1 cut(s) 47
Fsp4HI GCNGC 8 cut(s) 6, 35, 195, 198, 336, 372, 435, 618
FspBI CTAG 1 cut(s) 410
GluI GCNGC 8 cut(s) 6, 35, 195, 198, 336, 372, 435, 618
GsaI CCCAGC 1 cut(s) 388
GsuI CTGGAG 1 cut(s) 633
HaeIII GGCC 3 cut(s) 84, 105, 434
HapII CCGG 1 cut(s) 54
Hin1II CATG 2 cut(s) 626, 697
HindIII AAGCTT 1 cut(s) 119
HinfI GANTC 4 cut(s) 64, 113, 346, 427
HpaII CCGG 1 cut(s) 54
HphI GGTGA 2 cut(s) 415, 522
Hpy188I TCNGA 7 cut(s) 112, 142, 171, 342, 368, 580, 649
Hpy188III TCNNGA 3 cut(s) 54, 221, 667
Hpy99I CGWCG 1 cut(s) 101
HpyAV CCTTC 1 cut(s) 140
HpyCH4III ACNGT 2 cut(s) 14, 327
HpyCH4IV ACGT 1 cut(s) 96
HpyCH4V TGCA 7 cut(s) 125, 197, 272, 320, 374, 448, 500
HpyF10VI GCNNNNNNNGC 2 cut(s) 368, 680
HpyF3I CTNAG 5 cut(s) 41, 170, 513, 579, 648
HpySE526I ACGT 1 cut(s) 96
Hsp92II CATG 2 cut(s) 626, 697
Kpn2I TCCGGA 1 cut(s) 53
Kzo9I GATC 1 cut(s) 478
LmnI GCTCC 3 cut(s) 368, 509, 614
Lsp1109I GCAGC 5 cut(s) 17, 181, 209, 322, 358
LweI GCATC 4 cut(s) 259, 299, 307, 457
MaeI CTAG 1 cut(s) 410
MaeII ACGT 1 cut(s) 96
MalI GATC 1 cut(s) 480
MbiI CCGCTC 1 cut(s) 617
MboI GATC 1 cut(s) 478
MboII GAAGA 2 cut(s) 326, 355
MhlI GDGCHC 1 cut(s) 47
MluCI AATT 4 cut(s) 288, 643, 656, 700
MlyI GAGTC 2 cut(s) 58, 355
MnlI CCTC 6 cut(s) 61, 165, 383, 463, 643, 673
MroI TCCGGA 1 cut(s) 53
MseI TTAA 4 cut(s) 246, 461, 486, 698
MspA1I CMGCKG 3 cut(s) 200, 437, 536
MspI CCGG 1 cut(s) 54
MspR9I CCNGG 3 cut(s) 60, 453, 612
Mva1269I GAATGC 2 cut(s) 23, 526
MvaI CCWGG 3 cut(s) 60, 453, 612
MwoI GCNNNNNNNGC 2 cut(s) 368, 680
NdeII GATC 1 cut(s) 478
NlaIII CATG 2 cut(s) 626, 697
NlaIV GGNNCC 2 cut(s) 83, 492
NspI RCATGY 1 cut(s) 697
PciI ACATGT 1 cut(s) 693
PctI GAATGC 2 cut(s) 23, 526
PfeI GAWTC 2 cut(s) 113, 427
PfoI TCCNGGA 1 cut(s) 610
PkrI GCNGC 8 cut(s) 7, 36, 196, 199, 337, 373, 436, 619
PleI GAGTC 2 cut(s) 58, 354
PpsI GAGTC 2 cut(s) 58, 354
PscI ACATGT 1 cut(s) 693
PshAI GACNNNNGTC 1 cut(s) 95
Psp124BI GAGCTC 1 cut(s) 47
Psp6I CCWGG 3 cut(s) 58, 451, 610
PspFI CCCAGC 1 cut(s) 384
PspGI CCWGG 3 cut(s) 58, 451, 610
PspN4I GGNNCC 2 cut(s) 83, 492
PspPI GGNCC 3 cut(s) 56, 82, 104
PstI CTGCAG 2 cut(s) 199, 376
PvuII CAGCTG 1 cut(s) 200
RsaI GTAC 1 cut(s) 641
RsaNI GTAC 1 cut(s) 640
SacI GAGCTC 1 cut(s) 47
SaqAI TTAA 4 cut(s) 246, 461, 486, 698
SatI GCNGC 8 cut(s) 6, 35, 195, 198, 336, 372, 435, 618
Sau3AI GATC 1 cut(s) 478
Sau96I GGNCC 3 cut(s) 56, 82, 104
SchI GAGTC 2 cut(s) 58, 355
ScrFI CCNGG 3 cut(s) 60, 453, 612
SduI GDGCHC 1 cut(s) 47
SfaNI GCATC 4 cut(s) 259, 299, 307, 457
SfcI CTRYAG 2 cut(s) 195, 372
SinI GGWCC 1 cut(s) 56
Sse9I AATT 4 cut(s) 288, 643, 656, 700
SspI AATATT 1 cut(s) 504
SspMI CTAG 1 cut(s) 410
SstI GAGCTC 1 cut(s) 47
StyD4I CCNGG 3 cut(s) 58, 451, 610
TaaI ACNGT 2 cut(s) 14, 327
TaiI ACGT 1 cut(s) 99
TaqI TCGA 3 cut(s) 267, 457, 477
TasI AATT 4 cut(s) 288, 643, 656, 700
TatI WGTACW 1 cut(s) 639
TauI GCSGC 3 cut(s) 37, 437, 620
TfiI GAWTC 2 cut(s) 113, 427
Tru1I TTAA 4 cut(s) 246, 461, 486, 698
Tru9I TTAA 4 cut(s) 246, 461, 486, 698
TscAI CASTG 2 cut(s) 19, 45
TseI GCWGC 5 cut(s) 5, 194, 197, 335, 371
TspDTI ATGAA 4 cut(s) 171, 281, 560, 669
TspGWI ACGGA 1 cut(s) 314
TspRI CASTG 2 cut(s) 19, 45
VpaK11BI GGWCC 1 cut(s) 56
XapI RAATTY 1 cut(s) 288
XceI RCATGY 1 cut(s) 697
XspI CTAG 1 cut(s) 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.