RchiOBHm_Chr4g0418681

1,4-beta-D-glucanase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000177
Physical Location & Seq
Forward (+)
44142928 .. 44147340
4413 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38866

Sequence Viewer

Length: 909 bp
ATGTGTGTGTTGTTCGTACCAGTGCCCCTCCTAGCTCGTGATCAGAGAAAGACAGAAGAAATGTCAGGCCCTCAGTGCTGCTCAAACCCACCGACCCTGAACACAAACAAAGACCCTCCGGCCATGTTGAGAAGCTTGGTGGTCTCGCCTCCTATCTCACAGGCTCTCCTGACTCCAAGCTTGCCATTGTTCTTGTCTCTGACGTTTTTGATTGTGTATTGTAGGTATGATGCTCCAAACTTGAGGAAGCTCACAGACAAAGTTGCAGCTGCTGGCTTCTTTGTTGTGGTCCCTGACTTCTTTTATGGAAACCCTTATGTACCTCCAGCTGATGGATCCCATGGCGGTATACCTACATGGCTGAAGGGTCATGGACCGGAGAAGGGATTTGAAGATGTTAAAGTAGTTATTGAAGCTCTAAAAAGTAAAGGTGTTTCTGCAATCGGTGCTGCAGGCTTCTGTTGGGGAGCCAAGGTTGTAGTTGAACTTGCAAAGGGTGACTTTATCCAAGCTGCGGTTCTGGCTCATCCTTCATTTGTCACTGTGGATGATATCAAGGCTGTTAAGGTTCCAATATCTATACTTGGAGCTGAGATTGATCGGTCATCTCCGCCTGAACTCGTGAAACAATTTGAAGAGGCTTTGACTGCAAAGTCTGAGGTAAAATATCCTTTCATCAAAGTTATAACACCAAGACCTGATAGGAAAGAGGCACTACCGTGGTATCCAGTAGTTATTGCAATTCTAGAGTTTGAAATGACTGTTGGTATCAAAAGTCACGTGAAGATATTCCCAAAAGTGTCGCATGGATGGACTGTGAGGTATGATGTTTCAGACAAAGAAGCTGTGAAGCCCGCAGAGGAGGCTCATAAAGATTTGTTGGAGTGGTTTGTGAGCCATGTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
ko00361 ko00364 ko00623 ko01100 ko01110 ko01120 ko01130 map00361 map00364 map00623 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
DLH

Protein Analysis

302

Amino Acids

33.14

Weight (kDa)

7.64

Isoelectric Point (pI)

35.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DLH PF01738 80 - 218 1.8e-17 Dienelactone hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000177)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23570 AT3G23570 AT3G23570 AT3G23600 AT3G23600
fragaria_vesca FvH4_2g23960 FvH4_2g23960 FvH4_2g23960 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14330 FvH4_6g28031 FvH4_6g29270
malus_domestica MD13G1181200.v1.1 MD13G1181300.v1.1 MD13G1181400.v1.1 MD13G1181500.v1.1 MD13G1181600.v1.1 MD13G1181700.v1.1 MD13G1181800.v1.1 MD13G1181900.v1.1 MD15G1403300.v1.1 MD15G1403400.v1.1 MD16G1181900.v1.1 MD16G1182000.v1.1 MD16G1182200.v1.1 MD16G1182300.v1.1 MD16G1182400.v1.1
prunus_persica Prupe.1G149400_v2.0.a1 Prupe.1G149600_v2.0.a1 Prupe.1G149700_v2.0.a1 Prupe.1G149800_v2.0.a1 Prupe.1G549400_v2.0.a1 Prupe.8G245600_v2.0.a1
pyrus_communis pycom13g15640 pycom13g15650 pycom13g15670 pycom13g15700 pycom15g36070 pycom16g15270 pycom16g15300 pycom16g15310
rosa_chinensis RchiOBHm_Chr1g0334461 RchiOBHm_Chr1g0334471 RchiOBHm_Chr2g0131731 RchiOBHm_Chr4g0417881 RchiOBHm_Chr4g0418561 RchiOBHm_Chr4g0418571 RchiOBHm_Chr4g0418581 RchiOBHm_Chr4g0418591 RchiOBHm_Chr4g0418601 RchiOBHm_Chr4g0418621 RchiOBHm_Chr4g0418651 RchiOBHm_Chr4g0418661 RchiOBHm_Chr4g0418671 RchiOBHm_Chr4g0418681 RchiOBHm_Chr4g0418691 RchiOBHm_Chr4g0418701 RchiOBHm_Chr4g0421371 RchiOBHm_Chr6g0291601 RchiOBHm_Chr6g0291611 RchiOBHm_Chr6g0291621 RchiOBHm_Chr6g0291631 RchiOBHm_Chr7g0229821
rosa_laevigata RLG00000007843 RLG00000007844 RLG00000007846 RLG00000007847 RLG00000007849 RLG00000012101 RLG00000012104 RLG00000029451
rosa_multiflora Rmu_sc0000363.1_g000035 Rmu_sc0000935.1_g000003 Rmu_sc0000935.1_g000010 Rmu_sc0002226.1_g000008 Rmu_sc0002226.1_g000014 Rmu_sc0002589.1_g000031 Rmu_sc0003443.1_g000009 Rmu_sc0006431.1_g000001 Rmu_sc0006431.1_g000006 Rmu_sc0006431.1_g000023 Rmu_sc0006705.1_g000014 Rmu_sc0006705.1_g000015 Rmu_sc0006705.1_g000016 Rmu_sc0006705.1_g000017 Rmu_sc0006705.1_g000018 Rmu_sc0014797.1_g000001 Rmu_sc0041885.1_g000001
rosa_roxburghii Rroxscaffold_4G00316820 Rroxscaffold_5G00361830 Rroxscaffold_5G00361840 Rroxscaffold_5G00361850 Rroxscaffold_5G00361860 Rroxscaffold_5G00361880 Rroxscaffold_5G00361890 Rroxscaffold_5G00361900 Rroxscaffold_5G00361910 Rroxscaffold_5G00361920 Rroxscaffold_5G00361930 Rroxscaffold_7G00175910 Rroxscaffold_7G00175930 Rroxscaffold_7G00175940
rosa_rugosa Rorug02G0299300 Rorug04G0154500 Rorug04G0154600 Rorug04G0154700 Rorug06G0220500
rosa_samantha Rh1AG006300 Rh1AG139200 Rh1AG139300 Rh1BG105600 Rh1CG131200 Rh1CG131300 Rh1DG143200 Rh2AG210000 Rh2CG337500 Rh2DG216200 Rh2DG376200 Rh4AG215900 Rh4AG216000 Rh4AG216100 Rh4AG216200 Rh4AG216300 Rh4AG216400 Rh4BG214300 Rh4BG214400 Rh4BG214500 Rh4BG214600 Rh4BG214700 Rh4BG214800 Rh4CG212300 Rh4CG227500 Rh4CG227700 Rh4CG227900 Rh4CG228000 Rh4CG228100 Rh4DG214200 Rh4DG215000 Rh4DG215200 Rh5AG059400 Rh6AG332900 Rh6AG333000 Rh6AG333100 Rh6BG339700 Rh6BG339900 Rh6CG346100 Rh6CG346200 Rh6DG333300 Rh6DG333400 Rh7AG435400 Rh7CG454500
rosa_wichuraiana Rw1G011480 Rw2G028580 Rw4G018270 Rw4G018280 Rw4G018290 Rw4G018360 Rw4G018390 Rw4G018400 Rw4G018410 Rw4G018420 Rw6G028910 Rw6G029000 Rw6G029010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 686
AasI GACNNNNNNGTC 1 cut(s) 652
AccB7I CCANNNNNTGG 1 cut(s) 332
AccI GTMKAC 1 cut(s) 349
AciI CCGC 4 cut(s) 345, 515, 611, 855
AclWI GGATC 2 cut(s) 330, 343
AcoI YGGCCR 1 cut(s) 120
AcuI CTGAAG 1 cut(s) 383
AcvI CACGTG 1 cut(s) 781
AfaI GTAC 2 cut(s) 18, 321
AfiI CCNNNNNNNGG 3 cut(s) 332, 383, 514
AgsI TTSAA 5 cut(s) 392, 413, 485, 635, 755
AjuI GAANNNNNNNTTGG 4 cut(s) 501, 533, 747, 779
AleI CACNNNNGTG 1 cut(s) 718
Alw26I GTCTC 2 cut(s) 148, 201
AlwI GGATC 2 cut(s) 330, 343
AlwNI CAGNNNCTG 1 cut(s) 272
AoxI GGCC 2 cut(s) 67, 120
ApeKI GCWGC 5 cut(s) 78, 266, 269, 449, 512
Asp700I GAANNNNTTC 1 cut(s) 788
AspS9I GGNCC 3 cut(s) 68, 289, 374
AsuHPI GGTGA 1 cut(s) 509
AvaII GGWCC 2 cut(s) 289, 374
BaeGI GKGCMC 1 cut(s) 27
BaeI ACNNNNGTAYC 2 cut(s) 707, 740
BamHI GGATCC 1 cut(s) 335
BauI CACGAG 2 cut(s) 36, 620
BbrPI CACGTG 1 cut(s) 781
BbvI GCAGC 5 cut(s) 65, 256, 278, 436, 499
BccI CCATC 2 cut(s) 326, 804
BciVI GTATCC 1 cut(s) 735
BclI TGATCA 1 cut(s) 40
BcoDI GTCTC 2 cut(s) 148, 201
BfaI CTAG 2 cut(s) 32, 746
BfmI CTRYAG 1 cut(s) 450
BfuI GTATCC 1 cut(s) 735
BisI GCNGC 5 cut(s) 79, 267, 270, 450, 513
BlsI GCNGC 5 cut(s) 80, 268, 271, 451, 514
Bme18I GGWCC 2 cut(s) 289, 374
BmgT120I GGNCC 3 cut(s) 68, 289, 374
BmiI GGNNCC 4 cut(s) 291, 337, 469, 570
BmsI GCATC 1 cut(s) 220
BpmI CTGGAG 1 cut(s) 309
BpuEI CTTGAG 1 cut(s) 262
BsaAI YACGTR 1 cut(s) 781
BsaI GGTCTC 1 cut(s) 148
BsaJI CCNNGG 3 cut(s) 340, 471, 719
BsaWI WCCGGW 1 cut(s) 376
BsaXI ACNNNNNCTCC 4 cut(s) 150, 180, 459, 489
Bsc4I CCNNNNNNNGG 3 cut(s) 332, 383, 514
Bse1I ACTGG 2 cut(s) 20, 728
BseDI CCNNGG 3 cut(s) 340, 471, 719
BseGI GGATG 3 cut(s) 526, 553, 815
BseLI CCNNNNNNNGG 3 cut(s) 332, 383, 514
BseMII CTCAG 3 cut(s) 86, 582, 648
BseNI ACTGG 2 cut(s) 20, 728
BseRI GAGGAG 1 cut(s) 875
BseSI GKGCMC 1 cut(s) 27
BseXI GCAGC 5 cut(s) 65, 256, 278, 436, 499
BshFI GGCC 2 cut(s) 69, 122
BsiSI CCGG 2 cut(s) 119, 377
BslFI GGGAC 1 cut(s) 275
BslI CCNNNNNNNGG 3 cut(s) 332, 383, 514
BsmAI GTCTC 2 cut(s) 148, 201
BsmFI GGGAC 1 cut(s) 275
BsnI GGCC 2 cut(s) 69, 122
Bso31I GGTCTC 1 cut(s) 148
Bsp1286I GDGCHC 1 cut(s) 27
Bsp143I GATC 3 cut(s) 40, 335, 598
Bsp19I CCATGG 1 cut(s) 340
BspACI CCGC 4 cut(s) 345, 515, 611, 855
BspANI GGCC 2 cut(s) 69, 122
BspCNI CTCAG 3 cut(s) 85, 583, 649
BspLI GGNNCC 4 cut(s) 291, 337, 469, 570
BspMAI CTGCAG 1 cut(s) 454
BspPI GGATC 2 cut(s) 330, 343
BspTNI GGTCTC 1 cut(s) 148
BsrI ACTGG 2 cut(s) 20, 728
BssECI CCNNGG 3 cut(s) 340, 471, 719
BssMI GATC 3 cut(s) 40, 335, 598
BssNAI GTATAC 1 cut(s) 350
BssSI CACGAG 2 cut(s) 36, 620
BssT1I CCWWGG 2 cut(s) 340, 471
Bst1107I GTATAC 1 cut(s) 350
Bst2BI CACGAG 2 cut(s) 36, 620
Bst4CI ACNGT 4 cut(s) 544, 720, 763, 817
Bst6I CTCTTC 1 cut(s) 630
BstAPI GCANNNNNTGC 1 cut(s) 446
BstBAI YACGTR 1 cut(s) 781
BstC8I GCNNGC 4 cut(s) 182, 274, 454, 855
BstDEI CTNAG 3 cut(s) 72, 591, 657
BstDSI CCRYGG 2 cut(s) 340, 719
BstF5I GGATG 3 cut(s) 526, 553, 815
BstKTI GATC 3 cut(s) 43, 338, 601
BstMAI GTCTC 2 cut(s) 148, 201
BstMBI GATC 3 cut(s) 40, 335, 598
BstMWI GCNNNNNNNGC 5 cut(s) 75, 446, 521, 647, 863
BstSFI CTRYAG 1 cut(s) 450
BstSLI GKGCMC 1 cut(s) 27
BstV1I GCAGC 5 cut(s) 65, 256, 278, 436, 499
BstX2I RGATCY 1 cut(s) 335
BstYI RGATCY 1 cut(s) 335
BstZ17I GTATAC 1 cut(s) 350
BsuI GTATCC 1 cut(s) 735
BsuRI GGCC 2 cut(s) 69, 122
BtgI CCRYGG 2 cut(s) 340, 719
BtsCI GGATG 3 cut(s) 526, 553, 815
BtsIMutI CAGTG 3 cut(s) 27, 80, 540
Cac8I GCNNGC 4 cut(s) 182, 274, 454, 855
CaiI CAGNNNCTG 1 cut(s) 272
Cfr13I GGNCC 3 cut(s) 68, 289, 374
Csp6I GTAC 2 cut(s) 17, 320
CviAII CATG 6 cut(s) 124, 341, 357, 371, 806, 899
CviQI GTAC 2 cut(s) 17, 320
DdeI CTNAG 3 cut(s) 72, 591, 657
DpnI GATC 3 cut(s) 42, 337, 600
DpnII GATC 3 cut(s) 40, 335, 598
DrdI GACNNNNNNGTC 1 cut(s) 652
DseDI GACNNNNNNGTC 1 cut(s) 652
EaeI YGGCCR 1 cut(s) 120
Eam1104I CTCTTC 1 cut(s) 630
EarI CTCTTC 1 cut(s) 630
EciI GGCGGA 1 cut(s) 600
Eco130I CCWWGG 2 cut(s) 340, 471
Eco31I GGTCTC 1 cut(s) 148
Eco32I GATATC 1 cut(s) 553
Eco47I GGWCC 2 cut(s) 289, 374
Eco57I CTGAAG 1 cut(s) 383
Eco72I CACGTG 1 cut(s) 781
EcoO109I RGGNCCY 1 cut(s) 68
EcoRV GATATC 1 cut(s) 553
EcoT14I CCWWGG 2 cut(s) 340, 471
ErhI CCWWGG 2 cut(s) 340, 471
FaeI CATG 6 cut(s) 127, 344, 360, 374, 809, 902
FalI AAGNNNNNCTT 2 cut(s) 485, 517
FaqI GGGAC 1 cut(s) 275
FatI CATG 6 cut(s) 123, 340, 356, 370, 805, 898
FauI CCCGC 1 cut(s) 862
FbaI TGATCA 1 cut(s) 40
FblI GTMKAC 1 cut(s) 349
Fnu4HI GCNGC 5 cut(s) 79, 267, 270, 450, 513
FokI GGATG 3 cut(s) 513, 560, 822
Fsp4HI GCNGC 5 cut(s) 79, 267, 270, 450, 513
FspBI CTAG 2 cut(s) 32, 746
GluI GCNGC 5 cut(s) 79, 267, 270, 450, 513
GsuI CTGGAG 1 cut(s) 309
HaeIII GGCC 2 cut(s) 69, 122
HapII CCGG 2 cut(s) 119, 377
Hin1II CATG 6 cut(s) 127, 344, 360, 374, 809, 902
HindIII AAGCTT 2 cut(s) 133, 178
HinfI GANTC 1 cut(s) 172
HpaII CCGG 2 cut(s) 119, 377
HphI GGTGA 1 cut(s) 509
Hpy166II GTNNAC 1 cut(s) 350
Hpy188I TCNGA 4 cut(s) 45, 201, 658, 835
Hpy188III TCNNGA 4 cut(s) 38, 169, 622, 746
Hpy8I GTNNAC 1 cut(s) 350
HpyAV CCTTC 3 cut(s) 358, 376, 540
HpyCH4III ACNGT 4 cut(s) 544, 720, 763, 817
HpyCH4IV ACGT 2 cut(s) 203, 780
HpyCH4V TGCA 6 cut(s) 266, 440, 452, 491, 650, 740
HpyF10VI GCNNNNNNNGC 5 cut(s) 75, 446, 521, 647, 863
HpyF3I CTNAG 3 cut(s) 72, 591, 657
HpySE526I ACGT 2 cut(s) 203, 780
Hsp92II CATG 6 cut(s) 127, 344, 360, 374, 809, 902
Ksp22I TGATCA 1 cut(s) 40
Kzo9I GATC 3 cut(s) 40, 335, 598
LmnI GCTCC 3 cut(s) 238, 467, 587
Lsp1109I GCAGC 5 cut(s) 65, 256, 278, 436, 499
LweI GCATC 1 cut(s) 220
MaeI CTAG 2 cut(s) 32, 746
MaeII ACGT 2 cut(s) 203, 780
MaeIII GTNAC 3 cut(s) 497, 538, 776
MalI GATC 3 cut(s) 42, 337, 600
MboI GATC 3 cut(s) 40, 335, 598
MboII GAAGA 4 cut(s) 68, 404, 647, 796
MflI RGATCY 1 cut(s) 335
MhlI GDGCHC 1 cut(s) 27
MluCI AATT 2 cut(s) 629, 741
MlyI GAGTC 1 cut(s) 166
MmeI TCCRAC 1 cut(s) 861
MroXI GAANNNNTTC 1 cut(s) 788
MseI TTAA 2 cut(s) 399, 564
MslI CAYNNNNRTG 1 cut(s) 718
MspA1I CMGCKG 2 cut(s) 269, 329
MspI CCGG 2 cut(s) 119, 377
MwoI GCNNNNNNNGC 5 cut(s) 75, 446, 521, 647, 863
NcoI CCATGG 1 cut(s) 340
NdeII GATC 3 cut(s) 40, 335, 598
NlaIII CATG 6 cut(s) 127, 344, 360, 374, 809, 902
NlaIV GGNNCC 4 cut(s) 291, 337, 469, 570
NmuCI GTSAC 3 cut(s) 497, 538, 776
OliI CACNNNNGTG 1 cut(s) 718
PdmI GAANNNNTTC 1 cut(s) 788
PflMI CCANNNNNTGG 1 cut(s) 332
PkrI GCNGC 5 cut(s) 80, 268, 271, 451, 514
PleI GAGTC 1 cut(s) 166
PmaCI CACGTG 1 cut(s) 781
PmlI CACGTG 1 cut(s) 781
PpsI GAGTC 1 cut(s) 166
Ppu21I YACGTR 1 cut(s) 781
PsiI TTATAA 1 cut(s) 686
PspCI CACGTG 1 cut(s) 781
PspN4I GGNNCC 4 cut(s) 291, 337, 469, 570
PspPI GGNCC 3 cut(s) 68, 289, 374
PstI CTGCAG 1 cut(s) 454
PstNI CAGNNNCTG 1 cut(s) 272
PsuI RGATCY 1 cut(s) 335
PvuII CAGCTG 2 cut(s) 269, 329
RsaI GTAC 2 cut(s) 18, 321
RsaNI GTAC 2 cut(s) 17, 320
RseI CAYNNNNRTG 1 cut(s) 718
SaqAI TTAA 2 cut(s) 399, 564
SatI GCNGC 5 cut(s) 79, 267, 270, 450, 513
Sau3AI GATC 3 cut(s) 40, 335, 598
Sau96I GGNCC 3 cut(s) 68, 289, 374
SchI GAGTC 1 cut(s) 166
SduI GDGCHC 1 cut(s) 27
SfaNI GCATC 1 cut(s) 220
SfcI CTRYAG 1 cut(s) 450
SinI GGWCC 2 cut(s) 289, 374
SmiMI CAYNNNNRTG 1 cut(s) 718
SmlI CTYRAG 1 cut(s) 241
SmoI CTYRAG 1 cut(s) 241
Sse9I AATT 2 cut(s) 629, 741
SsiI CCGC 4 cut(s) 345, 515, 611, 855
SspMI CTAG 2 cut(s) 32, 746
StyI CCWWGG 2 cut(s) 340, 471
TaaI ACNGT 4 cut(s) 544, 720, 763, 817
TaiI ACGT 2 cut(s) 206, 783
TaqII GACCGA 1 cut(s) 591
TasI AATT 2 cut(s) 629, 741
Tru1I TTAA 2 cut(s) 399, 564
Tru9I TTAA 2 cut(s) 399, 564
TscAI CASTG 3 cut(s) 27, 80, 547
TseFI GTSAC 3 cut(s) 497, 538, 776
TseI GCWGC 5 cut(s) 78, 266, 269, 449, 512
Tsp45I GTSAC 3 cut(s) 497, 538, 776
TspDTI ATGAA 2 cut(s) 522, 664
TspRI CASTG 3 cut(s) 27, 80, 547
Van91I CCANNNNNTGG 1 cut(s) 332
VpaK11BI GGWCC 2 cut(s) 289, 374
XbaI TCTAGA 1 cut(s) 745
XmiI GTMKAC 1 cut(s) 349
XmnI GAANNNNTTC 1 cut(s) 788
XspI CTAG 2 cut(s) 32, 746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.