Rh4CG227700

1,4-beta-D-glucanase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000177
Physical Location & Seq
Forward (+)
46487940 .. 46494733
6794 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG227700.1

Sequence Viewer

Length: 1008 bp
ATGTCAGGCCCTCAGTGCTGCTCGAACCCACCGACCCTGAACCCAACCAGTGGATCCGGCCACATTGAGAAGCTTGGTGGTCTCGACTCCTATGTCACTGGCTCCCCTGACTCCAAGCTTGCCATTGTTCTTGTCTCCGACGTTTTTGGGTATGATGCTCCAAACTTGAGGAAGCTCGCAGACAAAGTTGCAGCTGCTGGCTTCTTTGTTGTGGTCCCTGACTTCTTTTATGGAGACCCTTATGTACCTCCAGCTGATGGATCCTGGGACTCTCTACCTACTTGGCTGAAAGGTCATGGACCGGAGAAGGGATTTGAAGATGTTAAACTAGTTATTGGAGCTCTAAAAAGTAAAGGCGTCTCTGCAATCGGTGCTGCAGGCTTCTGTTGGGGAGCCAAGGTTGTAGTTGAACTTGCAAAGGGTGACTTTATCCAAGCTGCTGTTCTGGCTCATCCTTCTTTAGTCACTGTGGATGATATCAAGGCTGTTAAGGTTCCAATATCTATACTTGGAGCTGAGATTGATCAGATGTCTCCGCCTGAAGTCGTGAAACAATTTGAAGAGGCTTTGGCTGCAAAGTCTGAGGAAAAAGGATACGAGGATGCCAAACCAGTCCTTGATGCTTTAAAGAGTCATGGTGTTTCTGCAAAGGGAGCTGCAGGATTTTGCTGGGGAGGGGTTGTAGTGGCAAAACTTGCAAAATCTGATGACATTAAGGCTGCTGTGATATTGCATCCTGGCCGGTTAGCAGATGAAGATATACATGAGGTTAAGGTTCATACTGCAATATTGGGAGCTGAGATTGACAAAACTTCACCCCCAGAACAGTTGAAACAATTTGGGGAGATTTTATCAGTAAGGTCTGAGGGCAAGATTGCGGATCAGGATTGGAGAGGATGGCAAAACCGCAAATCTTTTGGTCCAAAAAGCAGTGAGATTTTTCAAAGAACCAGTGTGTTAAAATCCATTTTGGATTTCTCTCGCGTCACAAATGAGAATGACATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
ko00361 ko00364 ko00623 ko01100 ko01110 ko01120 ko01130 map00361 map00364 map00623 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
DLH

Protein Analysis

335

Amino Acids

35.62

Weight (kDa)

5.46

Isoelectric Point (pI)

33.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DLH PF01738 29 - 194 5e-22 Dienelactone hydrolase family
DLH PF01738 195 - 283 2.3e-06 Dienelactone hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000177)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23570 AT3G23570 AT3G23570 AT3G23600 AT3G23600
fragaria_vesca FvH4_2g23960 FvH4_2g23960 FvH4_2g23960 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14311 FvH4_4g14330 FvH4_6g28031 FvH4_6g29270
malus_domestica MD13G1181200.v1.1 MD13G1181300.v1.1 MD13G1181400.v1.1 MD13G1181500.v1.1 MD13G1181600.v1.1 MD13G1181700.v1.1 MD13G1181800.v1.1 MD13G1181900.v1.1 MD15G1403300.v1.1 MD15G1403400.v1.1 MD16G1181900.v1.1 MD16G1182000.v1.1 MD16G1182200.v1.1 MD16G1182300.v1.1 MD16G1182400.v1.1
prunus_persica Prupe.1G149400_v2.0.a1 Prupe.1G149600_v2.0.a1 Prupe.1G149700_v2.0.a1 Prupe.1G149800_v2.0.a1 Prupe.1G549400_v2.0.a1 Prupe.8G245600_v2.0.a1
pyrus_communis pycom13g15640 pycom13g15650 pycom13g15670 pycom13g15700 pycom15g36070 pycom16g15270 pycom16g15300 pycom16g15310
rosa_chinensis RchiOBHm_Chr1g0334461 RchiOBHm_Chr1g0334471 RchiOBHm_Chr2g0131731 RchiOBHm_Chr4g0417881 RchiOBHm_Chr4g0418561 RchiOBHm_Chr4g0418571 RchiOBHm_Chr4g0418581 RchiOBHm_Chr4g0418591 RchiOBHm_Chr4g0418601 RchiOBHm_Chr4g0418621 RchiOBHm_Chr4g0418651 RchiOBHm_Chr4g0418661 RchiOBHm_Chr4g0418671 RchiOBHm_Chr4g0418681 RchiOBHm_Chr4g0418691 RchiOBHm_Chr4g0418701 RchiOBHm_Chr4g0421371 RchiOBHm_Chr6g0291601 RchiOBHm_Chr6g0291611 RchiOBHm_Chr6g0291621 RchiOBHm_Chr6g0291631 RchiOBHm_Chr7g0229821
rosa_laevigata RLG00000007843 RLG00000007844 RLG00000007846 RLG00000007847 RLG00000007849 RLG00000012101 RLG00000012104 RLG00000029451
rosa_multiflora Rmu_sc0000363.1_g000035 Rmu_sc0000935.1_g000003 Rmu_sc0000935.1_g000010 Rmu_sc0002226.1_g000008 Rmu_sc0002226.1_g000014 Rmu_sc0002589.1_g000031 Rmu_sc0003443.1_g000009 Rmu_sc0006431.1_g000001 Rmu_sc0006431.1_g000006 Rmu_sc0006431.1_g000023 Rmu_sc0006705.1_g000014 Rmu_sc0006705.1_g000015 Rmu_sc0006705.1_g000016 Rmu_sc0006705.1_g000017 Rmu_sc0006705.1_g000018 Rmu_sc0014797.1_g000001 Rmu_sc0041885.1_g000001
rosa_roxburghii Rroxscaffold_4G00316820 Rroxscaffold_5G00361830 Rroxscaffold_5G00361840 Rroxscaffold_5G00361850 Rroxscaffold_5G00361860 Rroxscaffold_5G00361880 Rroxscaffold_5G00361890 Rroxscaffold_5G00361900 Rroxscaffold_5G00361910 Rroxscaffold_5G00361920 Rroxscaffold_5G00361930 Rroxscaffold_7G00175910 Rroxscaffold_7G00175930 Rroxscaffold_7G00175940
rosa_rugosa Rorug02G0299300 Rorug04G0154500 Rorug04G0154600 Rorug04G0154700 Rorug06G0220500
rosa_samantha Rh1AG006300 Rh1AG139200 Rh1AG139300 Rh1BG105600 Rh1CG131200 Rh1CG131300 Rh1DG143200 Rh2AG210000 Rh2CG337500 Rh2DG216200 Rh2DG376200 Rh4AG215900 Rh4AG216000 Rh4AG216100 Rh4AG216200 Rh4AG216300 Rh4AG216400 Rh4BG214300 Rh4BG214400 Rh4BG214500 Rh4BG214600 Rh4BG214700 Rh4BG214800 Rh4CG212300 Rh4CG227500 Rh4CG227700 Rh4CG227900 Rh4CG228000 Rh4CG228100 Rh4DG214200 Rh4DG215000 Rh4DG215200 Rh5AG059400 Rh6AG332900 Rh6AG333000 Rh6AG333100 Rh6BG339700 Rh6BG339900 Rh6CG346100 Rh6CG346200 Rh6DG333300 Rh6DG333400 Rh7AG435400 Rh7CG454500
rosa_wichuraiana Rw1G011480 Rw2G028580 Rw4G018270 Rw4G018280 Rw4G018290 Rw4G018360 Rw4G018390 Rw4G018400 Rw4G018410 Rw4G018420 Rw6G028910 Rw6G029000 Rw6G029010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 92
AccB7I CCANNNNNTGG 2 cut(s) 50, 257
AccII CGCG 1 cut(s) 984
AciI CCGC 3 cut(s) 536, 878, 907
AclWI GGATC 5 cut(s) 48, 61, 255, 268, 888
AcoI YGGCCR 2 cut(s) 58, 739
AcuI CTGAAG 1 cut(s) 561
AcyI GRCGYC 1 cut(s) 357
AfaI GTAC 1 cut(s) 246
AfiI CCNNNNNNNGG 3 cut(s) 50, 257, 308
AgsI TTSAA 5 cut(s) 317, 410, 560, 832, 944
AhlI ACTAGT 1 cut(s) 328
AjnI CCWGG 2 cut(s) 263, 736
AjuI GAANNNNNNNTTGG 2 cut(s) 426, 458
Alw21I GWGCWC 1 cut(s) 343
Alw26I GTCTC 5 cut(s) 86, 139, 228, 364, 537
AlwI GGATC 5 cut(s) 48, 61, 255, 268, 888
AlwNI CAGNNNCTG 1 cut(s) 197
AoxI GGCC 3 cut(s) 7, 58, 739
ApeKI GCWGC 8 cut(s) 18, 191, 194, 374, 437, 572, 656, 719
AspS9I GGNCC 4 cut(s) 8, 214, 299, 920
AsuHPI GGTGA 2 cut(s) 434, 807
AvaII GGWCC 3 cut(s) 214, 299, 920
BamHI GGATCC 2 cut(s) 53, 260
BanII GRGCYC 1 cut(s) 343
Bbv12I GWGCWC 1 cut(s) 343
BbvI GCAGC 8 cut(s) 5, 181, 203, 361, 424, 559, 643, 706
BccI CCATC 2 cut(s) 251, 891
BciT130I CCWGG 2 cut(s) 265, 738
BciVI GTATCC 1 cut(s) 587
BclI TGATCA 1 cut(s) 523
BcoDI GTCTC 5 cut(s) 86, 139, 228, 364, 537
BcuI ACTAGT 1 cut(s) 328
BfaI CTAG 1 cut(s) 329
BfmI CTRYAG 2 cut(s) 375, 657
BfuI GTATCC 1 cut(s) 587
BisI GCNGC 8 cut(s) 19, 192, 195, 375, 438, 573, 657, 720
BlsI GCNGC 8 cut(s) 20, 193, 196, 376, 439, 574, 658, 721
Bme1390I CCNGG 2 cut(s) 265, 738
Bme18I GGWCC 3 cut(s) 214, 299, 920
BmgT120I GGNCC 4 cut(s) 8, 214, 299, 920
BmiI GGNNCC 6 cut(s) 55, 103, 216, 262, 394, 495
BmrFI CCNGG 2 cut(s) 265, 738
BmsI GCATC 4 cut(s) 145, 592, 610, 742
BpmI CTGGAG 1 cut(s) 234
BpuEI CTTGAG 1 cut(s) 187
BsaHI GRCGYC 1 cut(s) 357
BsaI GGTCTC 2 cut(s) 86, 228
BsaJI CCNNGG 2 cut(s) 264, 396
BsaWI WCCGGW 1 cut(s) 301
BsaXI ACNNNNNCTCC 4 cut(s) 384, 414, 666, 696
Bsc4I CCNNNNNNNGG 3 cut(s) 50, 257, 308
Bse118I RCCGGY 1 cut(s) 741
Bse1I ACTGG 4 cut(s) 48, 103, 611, 951
BseBI CCWGG 2 cut(s) 265, 738
BseDI CCNNGG 2 cut(s) 264, 396
BseGI GGATG 5 cut(s) 451, 478, 607, 733, 902
BseLI CCNNNNNNNGG 3 cut(s) 50, 257, 308
BseMII CTCAG 5 cut(s) 26, 507, 573, 789, 855
BseNI ACTGG 4 cut(s) 48, 103, 611, 951
BseXI GCAGC 8 cut(s) 5, 181, 203, 361, 424, 559, 643, 706
BseYI CCCAGC 1 cut(s) 669
Bsh1236I CGCG 1 cut(s) 984
BshFI GGCC 3 cut(s) 9, 60, 741
BsiHKAI GWGCWC 1 cut(s) 343
BsiSI CCGG 3 cut(s) 57, 302, 742
BslFI GGGAC 2 cut(s) 200, 281
BslI CCNNNNNNNGG 3 cut(s) 50, 257, 308
BsmAI GTCTC 5 cut(s) 86, 139, 228, 364, 537
BsmBI CGTCTC 1 cut(s) 364
BsmFI GGGAC 2 cut(s) 200, 281
BsnI GGCC 3 cut(s) 9, 60, 741
Bso31I GGTCTC 2 cut(s) 86, 228
Bsp1286I GDGCHC 1 cut(s) 343
Bsp143I GATC 4 cut(s) 53, 260, 523, 880
BspACI CCGC 3 cut(s) 536, 878, 907
BspANI GGCC 3 cut(s) 9, 60, 741
BspCNI CTCAG 5 cut(s) 25, 508, 574, 790, 856
BspFNI CGCG 1 cut(s) 984
BspLI GGNNCC 6 cut(s) 55, 103, 216, 262, 394, 495
BspMAI CTGCAG 2 cut(s) 379, 661
BspPI GGATC 5 cut(s) 48, 61, 255, 268, 888
BspTNI GGTCTC 2 cut(s) 86, 228
BsrFI RCCGGY 1 cut(s) 741
BsrI ACTGG 4 cut(s) 48, 103, 611, 951
BssAI RCCGGY 1 cut(s) 741
BssECI CCNNGG 2 cut(s) 264, 396
BssMI GATC 4 cut(s) 53, 260, 523, 880
BssNI GRCGYC 1 cut(s) 357
BssT1I CCWWGG 1 cut(s) 396
Bst2UI CCWGG 2 cut(s) 265, 738
Bst4CI ACNGT 2 cut(s) 469, 828
Bst6I CTCTTC 1 cut(s) 555
BstACI GRCGYC 1 cut(s) 357
BstAPI GCANNNNNTGC 2 cut(s) 371, 695
BstC8I GCNNGC 4 cut(s) 120, 177, 199, 379
BstDEI CTNAG 5 cut(s) 12, 516, 582, 798, 864
BstF5I GGATG 5 cut(s) 451, 478, 607, 733, 902
BstFNI CGCG 1 cut(s) 984
BstKTI GATC 4 cut(s) 56, 263, 526, 883
BstMAI GTCTC 5 cut(s) 86, 139, 228, 364, 537
BstMBI GATC 4 cut(s) 53, 260, 523, 880
BstMWI GCNNNNNNNGC 6 cut(s) 15, 371, 446, 572, 653, 695
BstNI CCWGG 2 cut(s) 265, 738
BstSCI CCNGG 2 cut(s) 263, 736
BstSFI CTRYAG 2 cut(s) 375, 657
BstUI CGCG 1 cut(s) 984
BstV1I GCAGC 8 cut(s) 5, 181, 203, 361, 424, 559, 643, 706
BstX2I RGATCY 2 cut(s) 53, 260
BstYI RGATCY 2 cut(s) 53, 260
BsuI GTATCC 1 cut(s) 587
BsuRI GGCC 3 cut(s) 9, 60, 741
BtsCI GGATG 5 cut(s) 451, 478, 607, 733, 902
BtsI GCAGTG 1 cut(s) 937
BtsIMutI CAGTG 6 cut(s) 20, 55, 96, 465, 937, 958
Cac8I GCNNGC 4 cut(s) 120, 177, 199, 379
CaiI CAGNNNCTG 1 cut(s) 197
Cfr10I RCCGGY 1 cut(s) 741
Cfr13I GGNCC 4 cut(s) 8, 214, 299, 920
CseI GACGC 2 cut(s) 346, 973
Csp6I GTAC 1 cut(s) 245
CviAII CATG 3 cut(s) 296, 635, 764
CviQI GTAC 1 cut(s) 245
DdeI CTNAG 5 cut(s) 12, 516, 582, 798, 864
DpnI GATC 4 cut(s) 55, 262, 525, 882
DpnII GATC 4 cut(s) 53, 260, 523, 880
DraI TTTAAA 1 cut(s) 627
DrdI GACNNNNNNGTC 1 cut(s) 92
DseDI GACNNNNNNGTC 1 cut(s) 92
EaeI YGGCCR 2 cut(s) 58, 739
Eam1104I CTCTTC 1 cut(s) 555
EarI CTCTTC 1 cut(s) 555
EciI GGCGGA 1 cut(s) 525
Ecl136II GAGCTC 1 cut(s) 341
Eco130I CCWWGG 1 cut(s) 396
Eco24I GRGCYC 1 cut(s) 343
Eco31I GGTCTC 2 cut(s) 86, 228
Eco32I GATATC 1 cut(s) 478
Eco47I GGWCC 3 cut(s) 214, 299, 920
Eco53kI GAGCTC 1 cut(s) 341
Eco57I CTGAAG 1 cut(s) 561
EcoICRI GAGCTC 1 cut(s) 341
EcoO109I RGGNCCY 1 cut(s) 8
EcoRII CCWGG 2 cut(s) 263, 736
EcoRV GATATC 1 cut(s) 478
EcoT14I CCWWGG 1 cut(s) 396
EcoT38I GRGCYC 1 cut(s) 343
ErhI CCWWGG 1 cut(s) 396
Esp3I CGTCTC 1 cut(s) 364
FaeI CATG 3 cut(s) 299, 638, 767
FalI AAGNNNNNCTT 2 cut(s) 410, 442
FaqI GGGAC 2 cut(s) 200, 281
FatI CATG 3 cut(s) 295, 634, 763
FbaI TGATCA 1 cut(s) 523
Fnu4HI GCNGC 8 cut(s) 19, 192, 195, 375, 438, 573, 657, 720
FokI GGATG 5 cut(s) 438, 485, 614, 720, 909
FriOI GRGCYC 1 cut(s) 343
Fsp4HI GCNGC 8 cut(s) 19, 192, 195, 375, 438, 573, 657, 720
FspBI CTAG 1 cut(s) 329
GluI GCNGC 8 cut(s) 19, 192, 195, 375, 438, 573, 657, 720
GsaI CCCAGC 1 cut(s) 673
GsuI CTGGAG 1 cut(s) 234
HaeIII GGCC 3 cut(s) 9, 60, 741
HapII CCGG 3 cut(s) 57, 302, 742
HgaI GACGC 2 cut(s) 346, 973
Hin1I GRCGYC 1 cut(s) 357
Hin1II CATG 3 cut(s) 299, 638, 767
HindIII AAGCTT 2 cut(s) 71, 116
HinfI GANTC 4 cut(s) 86, 110, 269, 631
HpaII CCGG 3 cut(s) 57, 302, 742
HphI GGTGA 2 cut(s) 434, 807
Hpy188I TCNGA 5 cut(s) 139, 528, 583, 706, 865
Hpy188III TCNNGA 3 cut(s) 83, 547, 884
Hpy99I CGWCG 1 cut(s) 143
HpyAV CCTTC 2 cut(s) 301, 465
HpyCH4III ACNGT 2 cut(s) 469, 828
HpyCH4IV ACGT 1 cut(s) 141
HpyF10VI GCNNNNNNNGC 6 cut(s) 15, 371, 446, 572, 653, 695
HpyF3I CTNAG 5 cut(s) 12, 516, 582, 798, 864
HpySE526I ACGT 1 cut(s) 141
Hsp92I GRCGYC 1 cut(s) 357
Hsp92II CATG 3 cut(s) 299, 638, 767
Ksp22I TGATCA 1 cut(s) 523
Kzo9I GATC 4 cut(s) 53, 260, 523, 880
LmnI GCTCC 7 cut(s) 107, 163, 338, 392, 512, 653, 794
Lsp1109I GCAGC 8 cut(s) 5, 181, 203, 361, 424, 559, 643, 706
LweI GCATC 4 cut(s) 145, 592, 610, 742
MaeI CTAG 1 cut(s) 329
MaeII ACGT 1 cut(s) 141
MaeIII GTNAC 4 cut(s) 94, 422, 463, 985
MalI GATC 4 cut(s) 55, 262, 525, 882
MboI GATC 4 cut(s) 53, 260, 523, 880
MboII GAAGA 3 cut(s) 329, 572, 767
MflI RGATCY 2 cut(s) 53, 260
MhlI GDGCHC 1 cut(s) 343
MluCI AATT 2 cut(s) 554, 836
MlyI GAGTC 4 cut(s) 80, 104, 263, 640
MmeI TCCRAC 1 cut(s) 162
MseI TTAA 6 cut(s) 324, 489, 626, 714, 771, 959
MspA1I CMGCKG 2 cut(s) 194, 254
MspI CCGG 3 cut(s) 57, 302, 742
MspR9I CCNGG 2 cut(s) 265, 738
MvaI CCWGG 2 cut(s) 265, 738
MvnI CGCG 1 cut(s) 984
MwoI GCNNNNNNNGC 6 cut(s) 15, 371, 446, 572, 653, 695
NdeII GATC 4 cut(s) 53, 260, 523, 880
NlaIII CATG 3 cut(s) 299, 638, 767
NlaIV GGNNCC 6 cut(s) 55, 103, 216, 262, 394, 495
NmuCI GTSAC 4 cut(s) 94, 422, 463, 985
PcsI WCGNNNNNNNCGW 1 cut(s) 29
PflMI CCANNNNNTGG 2 cut(s) 50, 257
PkrI GCNGC 8 cut(s) 20, 193, 196, 376, 439, 574, 658, 721
PleI GAGTC 4 cut(s) 80, 104, 263, 639
PpsI GAGTC 4 cut(s) 80, 104, 263, 639
Psp124BI GAGCTC 1 cut(s) 343
Psp6I CCWGG 2 cut(s) 263, 736
PspFI CCCAGC 1 cut(s) 669
PspGI CCWGG 2 cut(s) 263, 736
PspN4I GGNNCC 6 cut(s) 55, 103, 216, 262, 394, 495
PspPI GGNCC 4 cut(s) 8, 214, 299, 920
PstI CTGCAG 2 cut(s) 379, 661
PstNI CAGNNNCTG 1 cut(s) 197
PsuI RGATCY 2 cut(s) 53, 260
PvuII CAGCTG 2 cut(s) 194, 254
RsaI GTAC 1 cut(s) 246
RsaNI GTAC 1 cut(s) 245
SacI GAGCTC 1 cut(s) 343
SaqAI TTAA 6 cut(s) 324, 489, 626, 714, 771, 959
SatI GCNGC 8 cut(s) 19, 192, 195, 375, 438, 573, 657, 720
Sau3AI GATC 4 cut(s) 53, 260, 523, 880
Sau96I GGNCC 4 cut(s) 8, 214, 299, 920
SchI GAGTC 4 cut(s) 80, 104, 263, 640
ScrFI CCNGG 2 cut(s) 265, 738
SduI GDGCHC 1 cut(s) 343
SfaNI GCATC 4 cut(s) 145, 592, 610, 742
SfcI CTRYAG 2 cut(s) 375, 657
SinI GGWCC 3 cut(s) 214, 299, 920
SmlI CTYRAG 1 cut(s) 166
SmoI CTYRAG 1 cut(s) 166
SpeI ACTAGT 1 cut(s) 328
Sse9I AATT 2 cut(s) 554, 836
SsiI CCGC 3 cut(s) 536, 878, 907
SspI AATATT 1 cut(s) 789
SspMI CTAG 1 cut(s) 329
SstI GAGCTC 1 cut(s) 343
StyD4I CCNGG 2 cut(s) 263, 736
StyI CCWWGG 1 cut(s) 396
TaaI ACNGT 2 cut(s) 469, 828
TaiI ACGT 1 cut(s) 144
TaqI TCGA 2 cut(s) 23, 84
TasI AATT 2 cut(s) 554, 836
Tru1I TTAA 6 cut(s) 324, 489, 626, 714, 771, 959
Tru9I TTAA 6 cut(s) 324, 489, 626, 714, 771, 959
TscAI CASTG 6 cut(s) 20, 55, 103, 472, 937, 958
TseFI GTSAC 4 cut(s) 94, 422, 463, 985
TseI GCWGC 8 cut(s) 18, 191, 194, 374, 437, 572, 656, 719
Tsp45I GTSAC 4 cut(s) 94, 422, 463, 985
TspDTI ATGAA 2 cut(s) 767, 768
TspRI CASTG 6 cut(s) 20, 55, 103, 472, 937, 958
Van91I CCANNNNNTGG 2 cut(s) 50, 257
VpaK11BI GGWCC 3 cut(s) 214, 299, 920
XspI CTAG 1 cut(s) 329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.