DNA-directed 5'-3' RNA polymerase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 485 |
| AccIII | TCCGGA | 1 cut(s) | 373 |
| AciI | CCGC | 1 cut(s) | 483 |
| AclWI | GGATC | 4 cut(s) | 282, 371, 384, 887 |
| AcsI | RAATTY | 2 cut(s) | 696, 1059 |
| AfaI | GTAC | 8 cut(s) | 59, 98, 225, 305, 592, 793, 1027, 1049 |
| AgsI | TTSAA | 7 cut(s) | 26, 164, 175, 423, 524, 749, 833 |
| AhlI | ACTAGT | 2 cut(s) | 65, 137 |
| AjiI | CACGTC | 1 cut(s) | 743 |
| AjnI | CCWGG | 2 cut(s) | 306, 989 |
| AluBI | AGCT | 3 cut(s) | 47, 150, 974 |
| AluI | AGCT | 3 cut(s) | 47, 150, 974 |
| AlwI | GGATC | 4 cut(s) | 282, 371, 384, 887 |
| Aor13HI | TCCGGA | 1 cut(s) | 373 |
| AoxI | GGCC | 2 cut(s) | 798, 1037 |
| ApeKI | GCWGC | 1 cut(s) | 113 |
| ApoI | RAATTY | 2 cut(s) | 696, 1059 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 736 |
| AspS9I | GGNCC | 3 cut(s) | 371, 798, 926 |
| AsuII | TTCGAA | 1 cut(s) | 700 |
| AsuNHI | GCTAGC | 1 cut(s) | 43 |
| AvaII | GGWCC | 2 cut(s) | 371, 926 |
| BaeGI | GKGCMC | 1 cut(s) | 921 |
| BamHI | GGATCC | 1 cut(s) | 376 |
| BanII | GRGCYC | 1 cut(s) | 257 |
| BauI | CACGAG | 1 cut(s) | 381 |
| BbsI | GAAGAC | 1 cut(s) | 737 |
| BbvI | GCAGC | 1 cut(s) | 125 |
| BceAI | ACGGC | 1 cut(s) | 173 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 848, 882 |
| BciT130I | CCWGG | 2 cut(s) | 308, 991 |
| BclI | TGATCA | 2 cut(s) | 760, 817 |
| BcuI | ACTAGT | 2 cut(s) | 65, 137 |
| BfaI | CTAG | 4 cut(s) | 44, 66, 138, 368 |
| BfmI | CTRYAG | 2 cut(s) | 354, 1017 |
| BglII | AGATCT | 1 cut(s) | 689 |
| BisI | GCNGC | 1 cut(s) | 114 |
| BlsI | GCNGC | 1 cut(s) | 115 |
| Bme1390I | CCNGG | 2 cut(s) | 308, 991 |
| Bme18I | GGWCC | 2 cut(s) | 371, 926 |
| BmgBI | CACGTC | 1 cut(s) | 743 |
| BmgT120I | GGNCC | 3 cut(s) | 371, 798, 926 |
| BmiI | GGNNCC | 1 cut(s) | 378 |
| BmrFI | CCNGG | 2 cut(s) | 308, 991 |
| BmsI | GCATC | 3 cut(s) | 340, 489, 759 |
| BmtI | GCTAGC | 1 cut(s) | 47 |
| BpiI | GAAGAC | 1 cut(s) | 737 |
| Bpu14I | TTCGAA | 1 cut(s) | 700 |
| BpuEI | CTTGAG | 1 cut(s) | 471 |
| BsaJI | CCNNGG | 3 cut(s) | 583, 922, 990 |
| BsaWI | WCCGGW | 1 cut(s) | 373 |
| Bse1I | ACTGG | 1 cut(s) | 594 |
| BseAI | TCCGGA | 1 cut(s) | 373 |
| BseBI | CCWGG | 2 cut(s) | 308, 991 |
| BseDI | CCNNGG | 3 cut(s) | 583, 922, 990 |
| BseGI | GGATG | 2 cut(s) | 774, 820 |
| BseMII | CTCAG | 2 cut(s) | 270, 998 |
| BseNI | ACTGG | 1 cut(s) | 594 |
| BseSI | GKGCMC | 1 cut(s) | 921 |
| BseXI | GCAGC | 1 cut(s) | 125 |
| BshFI | GGCC | 2 cut(s) | 800, 1039 |
| BsiSI | CCGG | 1 cut(s) | 374 |
| BsmI | GAATGC | 2 cut(s) | 478, 604 |
| BsnI | GGCC | 2 cut(s) | 800, 1039 |
| Bsp119I | TTCGAA | 1 cut(s) | 700 |
| Bsp1286I | GDGCHC | 2 cut(s) | 257, 921 |
| Bsp13I | TCCGGA | 1 cut(s) | 373 |
| Bsp143I | GATC | 7 cut(s) | 274, 376, 560, 689, 760, 817, 892 |
| Bsp19I | CCATGG | 1 cut(s) | 583 |
| BspACI | CCGC | 1 cut(s) | 483 |
| BspANI | GGCC | 2 cut(s) | 800, 1039 |
| BspCNI | CTCAG | 2 cut(s) | 269, 999 |
| BspEI | TCCGGA | 1 cut(s) | 373 |
| BspHI | TCATGA | 1 cut(s) | 277 |
| BspLI | GGNNCC | 1 cut(s) | 378 |
| BspOI | GCTAGC | 1 cut(s) | 47 |
| BspPI | GGATC | 4 cut(s) | 282, 371, 384, 887 |
| BspT104I | TTCGAA | 1 cut(s) | 700 |
| BsrBI | CCGCTC | 1 cut(s) | 485 |
| BsrI | ACTGG | 1 cut(s) | 594 |
| BssECI | CCNNGG | 3 cut(s) | 583, 922, 990 |
| BssMI | GATC | 7 cut(s) | 274, 376, 560, 689, 760, 817, 892 |
| BssSI | CACGAG | 1 cut(s) | 381 |
| BssT1I | CCWWGG | 2 cut(s) | 583, 922 |
| Bst2BI | CACGAG | 1 cut(s) | 381 |
| Bst2UI | CCWGG | 2 cut(s) | 308, 991 |
| Bst4CI | ACNGT | 1 cut(s) | 303 |
| BstBI | TTCGAA | 1 cut(s) | 700 |
| BstC8I | GCNNGC | 3 cut(s) | 45, 111, 716 |
| BstDEI | CTNAG | 2 cut(s) | 256, 1007 |
| BstDSI | CCRYGG | 1 cut(s) | 583 |
| BstF5I | GGATG | 2 cut(s) | 774, 820 |
| BstKTI | GATC | 7 cut(s) | 277, 379, 563, 692, 763, 820, 895 |
| BstMBI | GATC | 7 cut(s) | 274, 376, 560, 689, 760, 817, 892 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 40, 482 |
| BstNI | CCWGG | 2 cut(s) | 308, 991 |
| BstNSI | RCATGY | 1 cut(s) | 230 |
| BstSCI | CCNGG | 2 cut(s) | 306, 989 |
| BstSFI | CTRYAG | 2 cut(s) | 354, 1017 |
| BstSLI | GKGCMC | 1 cut(s) | 921 |
| BstV1I | GCAGC | 1 cut(s) | 125 |
| BstV2I | GAAGAC | 1 cut(s) | 737 |
| BstX2I | RGATCY | 3 cut(s) | 376, 689, 892 |
| BstYI | RGATCY | 3 cut(s) | 376, 689, 892 |
| BsuRI | GGCC | 2 cut(s) | 800, 1039 |
| BtgI | CCRYGG | 1 cut(s) | 583 |
| BtrI | CACGTC | 1 cut(s) | 743 |
| BtsCI | GGATG | 2 cut(s) | 774, 820 |
| BtsIMutI | CAGTG | 2 cut(s) | 601, 1004 |
| Cac8I | GCNNGC | 3 cut(s) | 45, 111, 716 |
| CciI | TCATGA | 1 cut(s) | 277 |
| Cfr13I | GGNCC | 3 cut(s) | 371, 798, 926 |
| Csp6I | GTAC | 8 cut(s) | 58, 97, 224, 304, 591, 792, 1026, 1048 |
| CviAII | CATG | 8 cut(s) | 31, 35, 227, 278, 584, 764, 914, 1035 |
| CviQI | GTAC | 8 cut(s) | 58, 97, 224, 304, 591, 792, 1026, 1048 |
| DdeI | CTNAG | 2 cut(s) | 256, 1007 |
| DpnI | GATC | 7 cut(s) | 276, 378, 562, 691, 762, 819, 894 |
| DpnII | GATC | 7 cut(s) | 274, 376, 560, 689, 760, 817, 892 |
| DraI | TTTAAA | 1 cut(s) | 342 |
| Eco130I | CCWWGG | 2 cut(s) | 583, 922 |
| Eco24I | GRGCYC | 1 cut(s) | 257 |
| Eco47I | GGWCC | 2 cut(s) | 371, 926 |
| EcoRII | CCWGG | 2 cut(s) | 306, 989 |
| EcoT14I | CCWWGG | 2 cut(s) | 583, 922 |
| EcoT22I | ATGCAT | 1 cut(s) | 36 |
| EcoT38I | GRGCYC | 1 cut(s) | 257 |
| ErhI | CCWWGG | 2 cut(s) | 583, 922 |
| FaeI | CATG | 8 cut(s) | 34, 38, 230, 281, 587, 767, 917, 1038 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 486, 518, 525, 557, 721, 753 |
| FatI | CATG | 8 cut(s) | 30, 34, 226, 277, 583, 763, 913, 1034 |
| FbaI | TGATCA | 2 cut(s) | 760, 817 |
| Fnu4HI | GCNGC | 1 cut(s) | 114 |
| FokI | GGATG | 2 cut(s) | 781, 827 |
| FriOI | GRGCYC | 1 cut(s) | 257 |
| Fsp4HI | GCNGC | 1 cut(s) | 114 |
| FspBI | CTAG | 4 cut(s) | 44, 66, 138, 368 |
| GluI | GCNGC | 1 cut(s) | 114 |
| HaeIII | GGCC | 2 cut(s) | 800, 1039 |
| HapII | CCGG | 1 cut(s) | 374 |
| Hin1II | CATG | 8 cut(s) | 34, 38, 230, 281, 587, 767, 917, 1038 |
| HindIII | AAGCTT | 1 cut(s) | 148 |
| HinfI | GANTC | 3 cut(s) | 26, 643, 884 |
| HpaII | CCGG | 1 cut(s) | 374 |
| Hpy188I | TCNGA | 5 cut(s) | 259, 565, 687, 694, 1055 |
| Hpy188III | TCNNGA | 4 cut(s) | 194, 278, 374, 929 |
| HpyAV | CCTTC | 9 cut(s) | 169, 244, 333, 381, 429, 566, 625, 782, 854 |
| HpyCH4III | ACNGT | 1 cut(s) | 303 |
| HpyCH4IV | ACGT | 1 cut(s) | 742 |
| HpyCH4V | TGCA | 7 cut(s) | 34, 155, 623, 772, 869, 875, 1034 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 40, 482 |
| HpyF3I | CTNAG | 2 cut(s) | 256, 1007 |
| HpySE526I | ACGT | 1 cut(s) | 742 |
| Hsp92II | CATG | 8 cut(s) | 34, 38, 230, 281, 587, 767, 917, 1038 |
| Kpn2I | TCCGGA | 1 cut(s) | 373 |
| Ksp22I | TGATCA | 2 cut(s) | 760, 817 |
| Kzo9I | GATC | 7 cut(s) | 274, 376, 560, 689, 760, 817, 892 |
| LmnI | GCTCC | 1 cut(s) | 410 |
| LpnPI | CCDG | 9 cut(s) | 207, 293, 320, 387, 607, 942, 976, 1003, 1005 |
| Lsp1109I | GCAGC | 1 cut(s) | 125 |
| LweI | GCATC | 3 cut(s) | 340, 489, 759 |
| MaeI | CTAG | 4 cut(s) | 44, 66, 138, 368 |
| MaeII | ACGT | 1 cut(s) | 742 |
| MaeIII | GTNAC | 2 cut(s) | 652, 722 |
| MalI | GATC | 7 cut(s) | 276, 378, 562, 691, 762, 819, 894 |
| MbiI | CCGCTC | 1 cut(s) | 485 |
| MboI | GATC | 7 cut(s) | 274, 376, 560, 689, 760, 817, 892 |
| MboII | GAAGA | 6 cut(s) | 176, 179, 256, 259, 545, 737 |
| MfeI | CAATTG | 2 cut(s) | 870, 1029 |
| MflI | RGATCY | 3 cut(s) | 376, 689, 892 |
| MhlI | GDGCHC | 2 cut(s) | 257, 921 |
| MluCI | AATT | 8 cut(s) | 170, 464, 696, 828, 833, 870, 1029, 1059 |
| MmeI | TCCRAC | 2 cut(s) | 427, 917 |
| MnlI | CCTC | 5 cut(s) | 265, 377, 389, 567, 1006 |
| Mph1103I | ATGCAT | 1 cut(s) | 36 |
| MroI | TCCGGA | 1 cut(s) | 373 |
| MroXI | GAANNNNTTC | 1 cut(s) | 736 |
| MseI | TTAA | 4 cut(s) | 78, 341, 839, 1063 |
| MslI | CAYNNNNRTG | 1 cut(s) | 231 |
| MspI | CCGG | 1 cut(s) | 374 |
| MspR9I | CCNGG | 2 cut(s) | 308, 991 |
| MunI | CAATTG | 2 cut(s) | 870, 1029 |
| Mva1269I | GAATGC | 2 cut(s) | 478, 604 |
| MvaI | CCWGG | 2 cut(s) | 308, 991 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 40, 482 |
| NcoI | CCATGG | 1 cut(s) | 583 |
| NdeII | GATC | 7 cut(s) | 274, 376, 560, 689, 760, 817, 892 |
| NheI | GCTAGC | 1 cut(s) | 43 |
| NlaIII | CATG | 8 cut(s) | 34, 38, 230, 281, 587, 767, 917, 1038 |
| NlaIV | GGNNCC | 1 cut(s) | 378 |
| NsiI | ATGCAT | 1 cut(s) | 36 |
| NspI | RCATGY | 1 cut(s) | 230 |
| NspV | TTCGAA | 1 cut(s) | 700 |
| PagI | TCATGA | 1 cut(s) | 277 |
| PctI | GAATGC | 2 cut(s) | 478, 604 |
| PdmI | GAANNNNTTC | 1 cut(s) | 736 |
| PfeI | GAWTC | 3 cut(s) | 26, 643, 884 |
| PkrI | GCNGC | 1 cut(s) | 115 |
| Psp6I | CCWGG | 2 cut(s) | 306, 989 |
| PspGI | CCWGG | 2 cut(s) | 306, 989 |
| PspN4I | GGNNCC | 1 cut(s) | 378 |
| PspPI | GGNCC | 3 cut(s) | 371, 798, 926 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 348, 380 |
| PsuI | RGATCY | 3 cut(s) | 376, 689, 892 |
| RsaI | GTAC | 8 cut(s) | 59, 98, 225, 305, 592, 793, 1027, 1049 |
| RsaNI | GTAC | 8 cut(s) | 58, 97, 224, 304, 591, 792, 1026, 1048 |
| RseI | CAYNNNNRTG | 1 cut(s) | 231 |
| SaqAI | TTAA | 4 cut(s) | 78, 341, 839, 1063 |
| SatI | GCNGC | 1 cut(s) | 114 |
| Sau3AI | GATC | 7 cut(s) | 274, 376, 560, 689, 760, 817, 892 |
| Sau96I | GGNCC | 3 cut(s) | 371, 798, 926 |
| ScrFI | CCNGG | 2 cut(s) | 308, 991 |
| SduI | GDGCHC | 2 cut(s) | 257, 921 |
| SfaNI | GCATC | 3 cut(s) | 340, 489, 759 |
| SfcI | CTRYAG | 2 cut(s) | 354, 1017 |
| SfuI | TTCGAA | 1 cut(s) | 700 |
| SinI | GGWCC | 2 cut(s) | 371, 926 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 231 |
| SmlI | CTYRAG | 1 cut(s) | 486 |
| SmoI | CTYRAG | 1 cut(s) | 486 |
| SpeI | ACTAGT | 2 cut(s) | 65, 137 |
| Sse9I | AATT | 8 cut(s) | 170, 464, 696, 828, 833, 870, 1029, 1059 |
| SsiI | CCGC | 1 cut(s) | 483 |
| SspMI | CTAG | 4 cut(s) | 44, 66, 138, 368 |
| StyD4I | CCNGG | 2 cut(s) | 306, 989 |
| StyI | CCWWGG | 2 cut(s) | 583, 922 |
| TaaI | ACNGT | 1 cut(s) | 303 |
| TaiI | ACGT | 1 cut(s) | 745 |
| TaqI | TCGA | 3 cut(s) | 559, 700, 982 |
| TasI | AATT | 8 cut(s) | 170, 464, 696, 828, 833, 870, 1029, 1059 |
| TatI | WGTACW | 4 cut(s) | 57, 96, 1025, 1047 |
| TfiI | GAWTC | 3 cut(s) | 26, 643, 884 |
| Tru1I | TTAA | 4 cut(s) | 78, 341, 839, 1063 |
| Tru9I | TTAA | 4 cut(s) | 78, 341, 839, 1063 |
| TscAI | CASTG | 2 cut(s) | 601, 1011 |
| TseI | GCWGC | 1 cut(s) | 113 |
| TspDTI | ATGAA | 6 cut(s) | 51, 249, 276, 294, 330, 899 |
| TspRI | CASTG | 2 cut(s) | 601, 1011 |
| VpaK11BI | GGWCC | 2 cut(s) | 371, 926 |
| XapI | RAATTY | 2 cut(s) | 696, 1059 |
| XceI | RCATGY | 1 cut(s) | 230 |
| XmnI | GAANNNNTTC | 1 cut(s) | 736 |
| XspI | CTAG | 4 cut(s) | 44, 66, 138, 368 |
| Zsp2I | ATGCAT | 1 cut(s) | 36 |